LOCUS       NM_003638               6547 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens integrin subunit alpha 8 (ITGA8), transcript variant
            1, mRNA.
ACCESSION   NM_003638 XM_167711
VERSION     NM_003638.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Pavlovic,N., Kelam,N., Racetin,A., Filipovic,N., Pogorelic,Z.,
            Prusac,I.K. and Vukojevic,K.
  TITLE     Expression Profiles of ITGA8 and VANGL2 Are Altered in Congenital
            Anomalies of the Kidney and Urinary Tract (CAKUT)
  JOURNAL   Molecules 29 (14), 3294 (2024)
   PUBMED   39064873
  REMARK    GeneRIF: Expression Profiles of ITGA8 and VANGL2 Are Altered in
            Congenital Anomalies of the Kidney and Urinary Tract (CAKUT).
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Ran,X.M., Yang,J., Wang,Z.Y., Xiao,L.Z., Deng,Y.P. and Zhang,K.Q.
  TITLE     M2 macrophage-derived exosomal circTMCO3 acts through miR-515-5p
            and ITGA8 to enhance malignancy in ovarian cancer
  JOURNAL   Commun Biol 7 (1), 583 (2024)
   PUBMED   38755265
  REMARK    GeneRIF: M2 macrophage-derived exosomal circTMCO3 acts through
            miR-515-5p and ITGA8 to enhance malignancy in ovarian cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6547)
  AUTHORS   Ma,X., Zhang,L., Liu,L., Ruan,D. and Wang,C.
  TITLE     Hypermethylated ITGA8 Facilitate Bladder Cancer Cell Proliferation
            and Metastasis
  JOURNAL   Appl Biochem Biotechnol 196 (1), 245-260 (2024)
   PUBMED   37119505
  REMARK    GeneRIF: Hypermethylated ITGA8 Facilitate Bladder Cancer Cell
            Proliferation and Metastasis.
REFERENCE   4  (bases 1 to 6547)
  AUTHORS   Li,X., Zhu,G., Li,Y., Huang,H., Chen,C., Wu,D., Cao,P., Shi,R.,
            Su,L., Zhang,R., Liu,H. and Chen,J.
  TITLE     LINC01798/miR-17-5p axis regulates ITGA8 and causes changes in
            tumor microenvironment and stemness in lung adenocarcinoma
  JOURNAL   Front Immunol 14, 1096818 (2023)
   PUBMED   36911684
  REMARK    GeneRIF: LINC01798/miR-17-5p axis regulates ITGA8 and causes
            changes in tumor microenvironment and stemness in lung
            adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6547)
  AUTHORS   Gomez-Conde,S., Dunand,O., Hummel,A., Moriniere,V., Gauthier,M.,
            Mesnard,L. and Heidet,L.
  TITLE     Bi-allelic pathogenic variants in ITGA8 cause slowly progressive
            renal disease of unknown etiology
  JOURNAL   Clin Genet 103 (1), 114-118 (2023)
   PUBMED   36089563
  REMARK    GeneRIF: Bi-allelic pathogenic variants in ITGA8 cause slowly
            progressive renal disease of unknown etiology.
REFERENCE   6  (bases 1 to 6547)
  AUTHORS   Hartner,A., Schocklmann,H., Prols,F., Muller,U. and Sterzel,R.B.
  TITLE     Alpha8 integrin in glomerular mesangial cells and in experimental
            glomerulonephritis
  JOURNAL   Kidney Int 56 (4), 1468-1480 (1999)
   PUBMED   10504498
REFERENCE   7  (bases 1 to 6547)
  AUTHORS   Denda,S., Reichardt,L.F. and Muller,U.
  TITLE     Identification of osteopontin as a novel ligand for the integrin
            alpha8 beta1 and potential roles for this integrin-ligand
            interaction in kidney morphogenesis
  JOURNAL   Mol Biol Cell 9 (6), 1425-1435 (1998)
   PUBMED   9614184
REFERENCE   8  (bases 1 to 6547)
  AUTHORS   Schnapp,L.M., Hatch,N., Ramos,D.M., Klimanskaya,I.V., Sheppard,D.
            and Pytela,R.
  TITLE     The human integrin alpha 8 beta 1 functions as a receptor for
            tenascin, fibronectin, and vitronectin
  JOURNAL   J Biol Chem 270 (39), 23196-23202 (1995)
   PUBMED   7559467
REFERENCE   9  (bases 1 to 6547)
  AUTHORS   Varnum-Finney,B., Venstrom,K., Muller,U., Kypta,R., Backus,C.,
            Chiquet,M. and Reichardt,L.F.
  TITLE     The integrin receptor alpha 8 beta 1 mediates interactions of
            embryonic chick motor and sensory neurons with tenascin-C
  JOURNAL   Neuron 14 (6), 1213-1222 (1995)
   PUBMED   7541634
REFERENCE   10 (bases 1 to 6547)
  AUTHORS   Schnapp,L.M., Breuss,J.M., Ramos,D.M., Sheppard,D. and Pytela,R.
  TITLE     Sequence and tissue distribution of the human integrin alpha 8
            subunit: a beta 1-associated alpha subunit expressed in smooth
            muscle cells
  JOURNAL   J Cell Sci 108 (Pt 2), 537-544 (1995)
   PUBMED   7768999
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB237365.1, DC425586.1,
            AK297740.1, L36531.1, AL590636.12 and BU735798.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003638.2.
            
            Summary: Integrins are heterodimeric transmembrane receptor
            proteins that mediate numerous cellular processes including cell
            adhesion, cytoskeletal rearrangement, and activation of cell
            signaling pathways. Integrins are composed of alpha and beta
            subunits. This gene encodes the alpha 8 subunit of the
            heterodimeric integrin alpha8beta1 protein. The encoded protein is
            a single-pass type 1 membrane protein that contains multiple FG-GAP
            repeats. This repeat is predicted to fold into a beta propeller
            structure. This gene regulates the recruitment of mesenchymal cells
            into epithelial structures, mediates cell-cell interactions, and
            regulates neurite outgrowth of sensory and motor neurons. The
            integrin alpha8beta1 protein thus plays an important role in
            wound-healing and organogenesis. Mutations in this gene have been
            associated with renal hypodysplasia/aplasia-1 (RHDA1) and with
            several animal models of chronic kidney disease. Alternate splicing
            results in multiple transcript variants encoding distinct isoforms.
            [provided by RefSeq, Apr 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.95988.1,
                                           SRR1660803.194784.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378076.4/ ENSP00000367316.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-102               DB237365.1         420-521
            103-150             DC425586.1         33-80
            151-715             AK297740.1         76-640
            716-1059            L36531.1           451-794
            1060-3131           AK297740.1         940-3011
            3132-3132           AL590636.12        74066-74066         c
            3133-3342           AK297740.1         3013-3222
            3343-6297           AL590636.12        57219-60173         c
            6298-6547           BU735798.1         23-272              c
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p13"
     gene            1..6547
                     /gene="ITGA8"
                     /note="integrin subunit alpha 8"
                     /db_xref="GeneID:8516"
                     /db_xref="HGNC:HGNC:6144"
                     /db_xref="MIM:604063"
     exon            1..360
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     CDS             152..3343
                     /gene="ITGA8"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; integrin, alpha 8"
                     /codon_start=1
                     /product="integrin alpha-8 isoform 1 preproprotein"
                     /protein_id="NP_003629.2"
                     /db_xref="CCDS:CCDS31155.1"
                     /db_xref="GeneID:8516"
                     /db_xref="HGNC:HGNC:6144"
                     /db_xref="MIM:604063"
                     /translation="MSPGASRGPRGSQAPLIAPLCCAAAALGMLLWSPACQAFNLDVE
                     KLTVYSGPKGSYFGYAVDFHIPDARTASVLVGAPKANTSQPDIVEGGAVYYCPWPAEG
                     SAQCRQIPFDTTNNRKIRVNGTKEPIEFKSNQWFGATVKAHKGKVVACAPLYHWRTLK
                     PTPEKDPVGTCYVAIQNFSAYAEFSPCRNSNADPEGQGYCQAGFSLDFYKNGDLIVGG
                     PGSFYWQGQVITASVADIIANYSFKDILRKLAGEKQTEVAPASYDDSYLGYSVAAGEF
                     TGDSQQELVAGIPRGAQNFGYVSIINSTDMTFIQNFTGEQMASYFGYTVVVSDVNSDG
                     LDDVLVGAPLFMEREFESNPREVGQIYLYLQVSSLLFRDPQILTGTETFGRFGSAMAH
                     LGDLNQDGYNDIAIGVPFAGKDQRGKVLIYNGNKDGLNTKPSQVLQGVWASHAVPSGF
                     GFTLRGDSDIDKNDYPDLIVGAFGTGKVAVYRARPVVTVDAQLLLHPMIINLENKTCQ
                     VPDSMTSAACFSLRVCASVTGQSIANTIVLMAEVQLDSLKQKGAIKRTLFLDNHQAHR
                     VFPLVIKRQKSHQCQDFIVYLRDETEFRDKLSPINISLNYSLDESTFKEGLEVKPILN
                     YYRENIVSEQAHILVDCGEDNLCVPDLKLSARPDKHQVIIGDENHLMLIINARNEGEG
                     AYEAELFVMIPEEADYVGIERNNKGFRPLSCEYKMENVTRMVVCDLGNPMVSGTNYSL
                     GLRFAVPRLEKTNMSINFDLQIRSSNKDNPDSNFVSLQINITAVAQVEIRGVSHPPQI
                     VLPIHNWEPEEEPHKEEEVGPLVEHIYELHNIGPSTISDTILEVGWPFSARDEFLLYI
                     FHIQTLGPLQCQPNPNINPQDIKPAASPEDTPELSAFLRNSTIPHLVRKRDVHVVEFH
                     RQSPAKILNCTNIECLQISCAVGRLEGGESAVLKVRSRLWAHTFLQRKNDPYALASLV
                     SFEVKKMPYTDQPAKLPEGSIVIKTSVIWATPNVSFSIPLWVIILAILLGLLVLAILT
                     LALWKCGFFDRARPPQEDMTDREQLTNDKTPEA"
     exon            361..494
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            495..595
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            596..719
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            720..781
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            782..827
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            828..953
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            954..998
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1042
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1099
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1152
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1358
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1550
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1596
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1704
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1760
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            1761..1915
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2053
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            2054..2121
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            2122..2269
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            2270..2362
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            2363..2442
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            2443..2523
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            2524..2629
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            2630..2788
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            2789..2917
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            2918..3031
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            3032..3133
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            3134..3256
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     exon            3257..6547
                     /gene="ITGA8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6527..6532
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ITGA8"
                     /note="hexamer: AATAAA"
     polyA_site      6547
                     /gene="ITGA8"
                     /note="major polyA site"
ORIGIN      
        1 agacgccggg cactgtcacc acgggtgcgc cgagcgcacc gacccgggac acgggcagct
       61 ggggaccgcc agattccacc agcccccctt gccccgcagg ggtcctcggc tcgcgctcct
      121 gggtagcagc cacccaccgg ggcggaggga gatgtcgccc ggggccagcc gcggtccccg
      181 gggaagccag gcgccgctga tcgcgcccct ctgctgcgcc gcggccgcgc tggggatgtt
      241 gctgtggtcc cccgcctgtc aggcgttcaa cctggacgtg gaaaagctca cagtgtacag
      301 cggccccaag ggcagctact tcggctacgc cgtggacttc cacatacccg acgcccgcac
      361 agcgagtgtc ttggtggggg cgcccaaagc caacaccagc cagcccgata tcgtggaagg
      421 gggagccgtc tattactgtc cttggcccgc ggaggggtct gcgcagtgca ggcagatacc
      481 gtttgacacc accaacaaca gaaagatcag agttaatgga accaaagaac ctatcgagtt
      541 caaatccaat cagtggtttg gagcaacagt gaaagctcac aaaggaaaag ttgtggcctg
      601 tgctccttta tatcactgga gaactcttaa accgacacca gaaaaggacc cagttggcac
      661 ctgctatgta gcaattcaga acttcagcgc ctatgccgag ttctctcctt gccggaacag
      721 caatgctgat ccggaaggcc agggttactg ccaagcagga tttagtctgg atttttataa
      781 gaatggagac cttattgtgg gaggacctgg gagtttctac tggcaaggac aagtgatcac
      841 tgccagtgtt gcagatatca ttgcaaatta ctcattcaag gatatcctca ggaaactggc
      901 aggagaaaag cagacggaag tggctccagc ttcctatgat gacagttacc ttggatactc
      961 agttgctgct ggggagttta ctggggattc tcagcaagaa ttggttgctg gaattccaag
     1021 aggagcacag aattttggat atgtttccat cattaactct acggatatga cgtttattca
     1081 gaatttcacg ggagaacaga tggcatctta ttttggatat accgttgtcg tatcagatgt
     1141 taacagtgat ggactggatg atgtcctggt tggggcacct ctctttatgg aacgtgaatt
     1201 tgagagcaac cccagagaag tagggcaaat ctacctgtat ttgcaagtga gctctctcct
     1261 cttcagagac ccccagatcc tcactggcac cgagacgttt gggagattcg gtagtgctat
     1321 ggcacactta ggagacctga accaagatgg atacaatgac attgccatcg gagtgccttt
     1381 tgcaggcaag gatcaaagag gcaaagtgct catttataat gggaacaaag atggcttaaa
     1441 caccaagcct tcccaagttc tgcaaggagt gtgggcctca catgctgtcc cttccggatt
     1501 tggctttact ttaagaggag attcagacat agacaagaat gattacccag atttgattgt
     1561 gggtgcattt ggaacaggaa aagtcgctgt ttacagagca agaccggttg tgactgtaga
     1621 tgcccagctt ctgctgcacc caatgattat caatcttgaa aataaaactt gccaggttcc
     1681 agactctatg acatctgctg cctgcttttc tttaagagta tgtgcatctg tcacaggcca
     1741 gagcattgca aacacaatag tcttgatggc agaggtgcaa ttagattccc tgaaacagaa
     1801 aggagctatt aaacggacgc tcttccttga taaccatcag gctcatcgcg tcttccctct
     1861 tgtgataaaa aggcagaaat cccaccagtg ccaggatttc atcgtttacc ttcgagatga
     1921 aactgaattc cgagataaat tatctccaat caacattagt ttgaattaca gtttggacga
     1981 atccaccttt aaagaaggcc tggaagtgaa accaatattg aactactaca gagaaaacat
     2041 tgttagtgaa caggctcaca ttctggtgga ctgtggagaa gacaatctgt gtgttcctga
     2101 cttgaagctg tcggctagac cagataagca tcaggtaatc attggagatg aaaatcacct
     2161 tatgctcata ataaatgcaa gaaatgaagg ggaaggagca tatgaagctg aactctttgt
     2221 aatgatacca gaagaggcag attatgttgg aatcgaacgc aacaacaagg gatttcgacc
     2281 actgagctgt gagtacaaga tggaaaatgt aaccaggatg gtggtgtgtg accttgggaa
     2341 ccctatggtg tctggaacaa attattccct gggcctccga tttgcagttc cacgtcttga
     2401 gaaaacaaac atgagcatta acttcgatct ccaaatcaga agttccaaca aggacaatcc
     2461 agacagcaat tttgtgagcc tgcaaatcaa catcactgct gtagcgcagg tggaaataag
     2521 aggagtgtca caccctccgc agattgttct gcccattcat aactgggaac cagaagagga
     2581 gccccacaaa gaggaggagg ttggaccatt ggtggaacat atttatgagc tgcacaatat
     2641 tggaccaagt accatcagtg acaccatcct ggaggtgggc tggcctttct ctgcccggga
     2701 tgaatttctt ctctatattt tccatattca aactctggga cctctgcagt gccaaccaaa
     2761 tcctaatatc aatccacagg atataaagcc tgctgcctcc ccagaggaca cccctgagct
     2821 cagcgccttt ttgcgaaact ctactattcc tcatcttgtc aggaagaggg atgtacatgt
     2881 ggtcgaattc cacagacaga gccctgcaaa aatactgaat tgtacaaata tcgagtgttt
     2941 acaaatctcc tgtgcagtgg gacgactcga aggaggagaa agcgcagtcc tgaaagtcag
     3001 gtcacgatta tgggcccaca ccttcctcca gagaaaaaat gatccctatg ctcttgcatc
     3061 cctggtgtcc tttgaagtta agaagatgcc ttatacagat cagccagcaa aactcccaga
     3121 aggaagcata gtaattaaga catcagttat ttgggcaact ccgaatgttt ccttctcaat
     3181 cccattatgg gtaataatac tagcaatact tcttggattg ttggttctcg ccattttaac
     3241 cttagcttta tggaagtgtg gattctttga cagagccaga cctcctcagg aggacatgac
     3301 cgacagggaa cagctgacaa atgacaagac ccctgaggca tgacaagaaa aaaaaagaag
     3361 accaaagacc tcaaacactg gtcctgttca aagaaaaaga aagaacatga gggttaaaaa
     3421 tcaaagcttt ctgatacctg acagtgaccc aggaaatgga ggggaccctg gaaacatcac
     3481 ctcatctaca ccgcactttg gagaaattgt cctgggtgcc cagtgagccc tgttggaaaa
     3541 ggaaacacca aagctggaga agccatcagc aattgtggaa gattttcttt tgctttaatc
     3601 gttctgtact tggcagacac tttgaaatgc gtatgggaac gtaagttgca gtccagatca
     3661 atccagcata agttgcagag atgaaatgtc tgaatcacta tagaatttac aaggtgaact
     3721 aaggtgaaat gactgattta ctatagaact tagaactaag accacaccat ccaacactac
     3781 tgtatccaat ggaacatttg acacctccct atggaaaaga aacatttcta aggacattat
     3841 ggctcaggga gacaagcaat atgttgttgg tactgtgaaa gtacttttga aaggacaaaa
     3901 ataatcttgt gagtgagggt gagagtttat ttatttattt tccatgtctc taggttgaga
     3961 atctgtcatc tagagaattt ggaaattaag atttggttag ctagttttca aaatcactca
     4021 ctcagtttcc tcctaagaac cactgtgtgc agataactca tttagcagag cttcttcaga
     4081 atttagacag tgctaaggaa aggaagattg gcattaggaa tcagttctat ttaatacctt
     4141 gctgccttcc atctgaaatt cctttcactc acattatcca gggactcaca tctgcttaat
     4201 ttagctttgc aagatgttgc atttatattt ccttgtatat ctttccattg acttagttaa
     4261 tacatgaatt tcgaggtact ctctaccttt ggagtgttta aaagtcatgg taaaatgtct
     4321 agagttcatt aattaaaaag caatttaaca ttatgggtaa acattatttt taatatctat
     4381 aagaagcaac acttcttata gatatttagc ttgggattaa atacccgttt ttcctgctat
     4441 gccttgtact tgtatacaca gagaaaacat gttgaatata acttagacaa aaacataaaa
     4501 ttatacaggc atgagccaaa ctgggaaaac tgattcctaa agatttttat ttaaaattgc
     4561 ttattccaac tttattactt atttttactg ctaatcttta taatatgtaa ctttggagaa
     4621 taatctttta tttaagctat tttcccatca tcttgaattt aaccatcaca aattttttat
     4681 gtttaaaact cttgtaaata attcataatc ttgtaatatt atcatttttg aagtaaaatt
     4741 gaccagccaa atttataggt agtctgcaca attttgtatc cttttttaat aatgaaaaat
     4801 tactatgaag aaatactgaa caaattttta tgtgcaatat tttatagacc tatgtatctg
     4861 aagcatgttt acactggcgt tttttttttt aattaatttc ctaaatgtta agtatgatag
     4921 aacaagctga cccaaatcct taagtttaca aagctgttgg aaaactttgt gtcctgattt
     4981 caacaatcac gctttgtttg aaagatgagc caagctcaca gacactaaat tttatgtcat
     5041 gccataagct ggagaggagc catttggcta cagctgcgga acttcattga ggagcaaatg
     5101 aaaggcacat ggacgagcac gctggtgcag ttcatgttct tcctgcctgt gaattgaata
     5161 ctgtcctggt agcagtttcg ggtcggtcag gagctcaagg ctggtttgtg tggctgacta
     5221 cggatgagca ctgaagttgc ctcaaagaat taaggggtgt ccacaccagc ctctgggggt
     5281 ctttggtgtt agtcttccag gtagagctgg ttttacaagt aggtggccat ctacaggatg
     5341 tgatgtgagc gatgccagac agctctctct gaccccaggt aatgccctga atctggtgat
     5401 cctggctgat ctgtgaccaa tagagattag ctccttggga tttggggtcc taaaaggtcc
     5461 ctgaaaaaat gcaccccttg tctttaagcc aacattggtg aaggaactga gaactcttag
     5521 ggttacataa aaaaagaccc ctgttgagat agtttatgca gatacctgga aggaactaga
     5581 gatgccagga ggaagactga gacccagaat gaagcaaggc atgagggctc caaagtgaac
     5641 tgcaccatcc cagagtctca gccaagatgg ccctttccta gagggacggg caccttccac
     5701 ctgcccacag gcactgctac ctatgggaat tgcagaaggc cgttgtacac acgcgcatgc
     5761 acgcagtggc cctctgccct ttagaatatg gaagcaaagt ctgggtgcag tgactcacac
     5821 ttgtaatccc aacactttgg gaggccgaga cgggcagatc acctgagttc aggagttcaa
     5881 gaccagcctg gccaacgtgg caaaacccca tctctactaa aaatacaaaa attagctggg
     5941 cgtggtggca gtctcctgta atcccagcta cctaggaggc tgaggcagag agagaatcgc
     6001 ttgaacccag gaggcggagg ttgcagtgag ctgagatcgc gcgactgcac tccagcctgg
     6061 gcgacagagg gaaactctgt ctcaacaaaa aaataaataa aataaaaaat aaaaaaagaa
     6121 tatggaagca aatggagaaa gggaggaaac tgaggtcagg caaaccttcc agcaatccgt
     6181 tctgcccaga gaacatactg acgacagaga acacactgag cgttatctgc ttctagttag
     6241 agcattgccg attgccttca taattcataa tgtgctgtgc tcctctgagg gtctatctgt
     6301 atttctcttc aaagaattcc ctttttaaat tcttgcagct gggttttaag acacaataat
     6361 ggggcaaatt aagttttata aacttggaga tttttataca gtaaataaaa aaataatatg
     6421 tactagtcac ccctgaaact gcattcttag aactcgactc ttgttttatt taaatacgtg
     6481 cgtagatatc tgtgaagatt ttcatgtacc tatttacctt gtcactaata aaattaaaac
     6541 tgaaaga
//
