LOCUS       NM_000885               6781 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens integrin subunit alpha 4 (ITGA4), transcript variant
            1, mRNA.
ACCESSION   NM_000885
VERSION     NM_000885.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Parte,S., Kaur,A.B., Nimmakayala,R.K., Ogunleye,A.O.,
            Chirravuri,R., Vengoji,R., Leon,F., Nallasamy,P., Rauth,S.,
            Alsafwani,Z.W., Lele,S., Cox,J.L., Bhat,I., Singh,S., Batra,S.K.
            and Ponnusamy,M.P.
  TITLE     Cancer-Associated Fibroblast Induces Acinar-to-Ductal Cell
            Transdifferentiation and Pancreatic Cancer Initiation Via
            LAMA5/ITGA4 Axis
  JOURNAL   Gastroenterology 166 (5), 842-858 (2024)
   PUBMED   38154529
  REMARK    GeneRIF: Cancer-Associated Fibroblast Induces Acinar-to-Ductal Cell
            Transdifferentiation and Pancreatic Cancer Initiation Via
            LAMA5/ITGA4 Axis.
REFERENCE   2  (bases 1 to 6781)
  AUTHORS   Zhang,D., Wu,H., Shao,X., Xiao,H. and Jiang,Y.
  TITLE     [Association of ITGA4 and ICAM-1 gene polymorphisms with the risk
            and clinicopathological characteristics of Crohn's disease]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 41 (2), 157-166 (2024)
   PUBMED   38311553
  REMARK    GeneRIF: [Association of ITGA4 and ICAM-1 gene polymorphisms with
            the risk and clinicopathological characteristics of Crohn's
            disease].
REFERENCE   3  (bases 1 to 6781)
  AUTHORS   Huang,M., Lu,L., Lin,C., Zheng,Y., Pan,X., Wang,S., Chen,S.,
            Zhang,Y., Liu,C., Ge,G., Zeng,Y.A. and Chen,J.
  TITLE     LRP12 is an endogenous transmembrane inactivator of alpha4
            integrins
  JOURNAL   Cell Rep 42 (6), 112667 (2023)
   PUBMED   37330909
  REMARK    GeneRIF: LRP12 is an endogenous transmembrane inactivator of alpha4
            integrins.
REFERENCE   4  (bases 1 to 6781)
  AUTHORS   Takada,Y.K., Simon,S.I. and Takada,Y.
  TITLE     The C-type lectin domain of CD62P (P-selectin) functions as an
            integrin ligand
  JOURNAL   Life Sci Alliance 6 (7), e202201747 (2023)
   PUBMED   37184585
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6781)
  AUTHORS   Shishido,S., Bonig,H. and Kim,Y.M.
  TITLE     Role of integrin alpha4 in drug resistance of leukemia
  JOURNAL   Front Oncol 4, 99 (2014)
   PUBMED   24904821
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6781)
  AUTHORS   Rubio,M., Nueda,A., Vara,A. and Corbi Lopez,A.L.
  TITLE     A single mRNA encodes the alpha 150 and alpha 80/70 forms of the
            alpha subunit of VLA4
  JOURNAL   Eur J Immunol 22 (4), 1099-1102 (1992)
   PUBMED   1551405
REFERENCE   7  (bases 1 to 6781)
  AUTHORS   Parker,C.M., Cepek,K.L., Russell,G.J., Shaw,S.K., Posnett,D.N.,
            Schwarting,R. and Brenner,M.B.
  TITLE     A family of beta 7 integrins on human mucosal lymphocytes
  JOURNAL   Proc Natl Acad Sci U S A 89 (5), 1924-1928 (1992)
   PUBMED   1542691
REFERENCE   8  (bases 1 to 6781)
  AUTHORS   Fernandez-Ruiz,E., Pardo-Manuel de Villena,F., Rubio,M.A.,
            Corbi,A.L., Rodriguez de Cordoba,S. and Sanchez-Madrid,F.
  TITLE     Mapping of the human VLA-alpha 4 gene to chromosome 2q31-q32
  JOURNAL   Eur J Immunol 22 (2), 587-590 (1992)
   PUBMED   1537388
REFERENCE   9  (bases 1 to 6781)
  AUTHORS   Ryan,D.H., Nuccie,B.L., Abboud,C.N. and Winslow,J.M.
  TITLE     Vascular cell adhesion molecule-1 and the integrin VLA-4 mediate
            adhesion of human B cell precursors to cultured bone marrow
            adherent cells
  JOURNAL   J Clin Invest 88 (3), 995-1004 (1991)
   PUBMED   1715889
REFERENCE   10 (bases 1 to 6781)
  AUTHORS   Takada,Y., Elices,M.J., Crouse,C. and Hemler,M.E.
  TITLE     The primary structure of the alpha 4 subunit of VLA-4: homology to
            other integrins and a possible cell-cell adhesion function
  JOURNAL   EMBO J 8 (5), 1361-1368 (1989)
   PUBMED   2788572
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC020595.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000885.5.
            
            Summary: The gene encodes a member of the integrin alpha chain
            family of proteins. Integrins are heterodimeric integral membrane
            proteins composed of an alpha chain and a beta chain that function
            in cell surface adhesion and signaling. The encoded preproprotein
            is proteolytically processed to generate light and heavy chains
            that comprise the alpha 4 subunit. This subunit associates with a
            beta 1 or beta 7 subunit to form an integrin that may play a role
            in cell motility and migration. This integrin is a therapeutic
            target for the treatment of multiple sclerosis, Crohn's disease and
            inflammatory bowel disease. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X16983.1, L12002.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397033.7/ ENSP00000380227.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-466               AC020595.7         146066-146531       c
            467-588             AC020595.7         145600-145721       c
            589-695             AC020595.7         128851-128957       c
            696-825             AC020595.7         128629-128758       c
            826-893             AC020595.7         125093-125160       c
            894-1023            AC020595.7         123651-123780       c
            1024-1109           AC020595.7         122234-122319       c
            1110-1172           AC020595.7         121495-121557       c
            1173-1310           AC020595.7         121266-121403       c
            1311-1422           AC020595.7         117925-118036       c
            1423-1517           AC020595.7         110498-110592       c
            1518-1608           AC020595.7         109105-109195       c
            1609-1654           AC020595.7         108501-108546       c
            1655-1809           AC020595.7         107980-108134       c
            1810-1964           AC020595.7         105140-105294       c
            1965-2114           AC020595.7         94110-94259         c
            2115-2191           AC020595.7         92142-92218         c
            2192-2342           AC020595.7         81576-81726         c
            2343-2438           AC020595.7         80385-80480         c
            2439-2518           AC020595.7         79667-79746         c
            2519-2608           AC020595.7         78626-78715         c
            2609-2699           AC020595.7         76530-76620         c
            2700-2807           AC020595.7         74269-74376         c
            2808-2933           AC020595.7         73268-73393         c
            2934-3053           AC020595.7         72141-72260         c
            3054-3152           AC020595.7         69547-69645         c
            3153-3272           AC020595.7         68982-69101         c
            3273-6781           AC020595.7         64977-68485         c
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.3"
     gene            1..6781
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /note="integrin subunit alpha 4"
                     /db_xref="GeneID:3676"
                     /db_xref="HGNC:HGNC:6140"
                     /db_xref="MIM:192975"
     exon            1..466
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     CDS             270..3368
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; antigen CD49D, alpha-4 subunit of VLA-4
                     receptor; very late activation protein 4 receptor, alpha 4
                     subunit; 269C wild type; integrin alpha-IV; VLA-4 subunit
                     alpha; CD49 antigen-like family member D; alpha 4 subunit
                     of VLA-4 receptor"
                     /codon_start=1
                     /product="integrin alpha-4 isoform 1 preproprotein"
                     /protein_id="NP_000876.3"
                     /db_xref="CCDS:CCDS42788.1"
                     /db_xref="GeneID:3676"
                     /db_xref="HGNC:HGNC:6140"
                     /db_xref="MIM:192975"
                     /translation="MAWEARREPGPRRAAVRETVMLLLCLGVPTGRPYNVDTESALLY
                     QGPHNTLFGYSVVLHSHGANRWLLVGAPTANWLANASVINPGAIYRCRIGKNPGQTCE
                     QLQLGSPNGEPCGKTCLEERDNQWLGVTLSRQPGENGSIVTCGHRWKNIFYIKNENKL
                     PTGGCYGVPPDLRTELSKRIAPCYQDYVKKFGENFASCQAGISSFYTKDLIVMGAPGS
                     SYWTGSLFVYNITTNKYKAFLDKQNQVKFGSYLGYSVGAGHFRSQHTTEVVGGAPQHE
                     QIGKAYIFSIDEKELNILHEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTIR
                     EEGRVFVYINSGSGAVMNAMETNLVGSDKYAARFGESIVNLGDIDNDGFEDVAIGAPQ
                     EDDLQGAIYIYNGRADGISSTFSQRIEGLQISKSLSMFGQSISGQIDADNNGYVDVAV
                     GAFRSDSAVLLRTRPVVIVDASLSHPESVNRTKFDCVENGWPSVCIDLTLCFSYKGKE
                     VPGYIVLFYNMSLDVNRKAESPPRFYFSSNGTSDVITGSIQVSSREANCRTHQAFMRK
                     DVRDILTPIQIEAAYHLGPHVISKRSTEEFPPLQPILQQKKEKDIMKKTINFARFCAH
                     ENCSADLQVSAKIGFLKPHENKTYLAVGSMKTLMLNVSLFNAGDDAYETTLHVKLPVG
                     LYFIKILELEEKQINCEVTDNSGVVQLDCSIGYIYVDHLSRIDISFLLDVSSLSRAEE
                     DLSITVHATCENEEEMDNLKHSRVTVAIPLKYEVKLTVHGFVNPTSFVYGSNDENEPE
                     TCMVEKMNLTFHVINTGNSMAPNVSVEIMVPNSFSPQTDKLFNILDVQTTTGECHFEN
                     YQRVCALEQQKSAMQTLKGIVRFLSKTDKRLLYCIKADPHCLNFLCNFGKMESGKEAS
                     VHIQLEGRPSILEMDETSALKFEIRATGFPEPNPRVIELNKDENVAHVLLEGLHHQRP
                     KRYFTIVIISSSLLLGLIVLLLISYVMWKAGFFKRQYKSILQEENRRDSWSYINSKSN
                     DD"
     exon            467..588
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            589..695
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            696..825
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            826..893
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1023
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1109
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1172
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1310
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1422
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1517
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1608
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1654
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1809
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1810..1964
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1965..2114
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2191
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2192..2342
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2343..2438
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2439..2518
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2519..2608
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2609..2699
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2700..2807
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..2933
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2934..3053
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            3054..3152
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            3153..3272
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     exon            3273..6781
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3417..3422
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /note="hexamer: AAGAAA"
     polyA_site      3450
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /note="major polyA site"
     regulatory      6499..6504
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /note="hexamer: AATAAA"
     polyA_site      6523
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /note="major polyA site"
     regulatory      6749..6754
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
                     /note="hexamer: AATAAA"
     polyA_site      6781
                     /gene="ITGA4"
                     /gene_synonym="CD49D; IA4"
ORIGIN      
        1 gaagcagcgc gagcacccga agctcccggc tggcggcaga aaccgggagt ggggccgggc
       61 gagtgcgcgg catcccaggc cggcccgaac gctccgcccg cggtgggccg acttcccctc
      121 ctcttccctc tctccttcct ttagcccgct ggcgccggac acgctgcgcc tcatctcttg
      181 gggcgttctt ccccgttggc caaccgtcgc atcccgtgca actttggggt agtggccgtt
      241 tagtgttgaa tgttccccac cgagagcgca tggcttggga agcgaggcgc gaacccggcc
      301 cccgaagggc cgccgtccgg gagacggtga tgctgttgct gtgcctgggg gtcccgaccg
      361 gccgccccta caacgtggac actgagagcg cgctgcttta ccagggcccc cacaacacgc
      421 tgttcggcta ctcggtcgtg ctgcacagcc acggggcgaa ccgatggctc ctagtgggtg
      481 cgcccactgc caactggctc gccaacgctt cagtgatcaa tcccggggcg atttacagat
      541 gcaggatcgg aaagaatccc ggccagacgt gcgaacagct ccagctgggt agccctaatg
      601 gagaaccttg tggaaagact tgtttggaag agagagacaa tcagtggttg ggggtcacac
      661 tttccagaca gccaggagaa aatggatcca tcgtgacttg tgggcataga tggaaaaata
      721 tattttacat aaagaatgaa aataagctcc ccactggtgg ttgctatgga gtgccccctg
      781 atttacgaac agaactgagt aaaagaatag ctccgtgtta tcaagattat gtgaaaaaat
      841 ttggagaaaa ttttgcatca tgtcaagctg gaatatccag tttttacaca aaggatttaa
      901 ttgtgatggg ggccccagga tcatcttact ggactggctc tctttttgtc tacaatataa
      961 ctacaaataa atacaaggct tttttagaca aacaaaatca agtaaaattt ggaagttatt
     1021 taggatattc agtcggagct ggtcattttc ggagccagca tactaccgaa gtagtcggag
     1081 gagctcctca acatgagcag attggtaagg catatatatt cagcattgat gaaaaagaac
     1141 taaatatctt acatgaaatg aaaggtaaaa agcttggatc gtactttgga gcttctgtct
     1201 gtgctgtgga cctcaatgca gatggcttct cagatctgct cgtgggagca cccatgcaga
     1261 gcaccatcag agaggaagga agagtgtttg tgtacatcaa ctctggctcg ggagcagtaa
     1321 tgaatgcaat ggaaacaaac ctcgttggaa gtgacaaata tgctgcaaga tttggggaat
     1381 ctatagttaa tcttggcgac attgacaatg atggctttga agatgttgct atcggagctc
     1441 cacaagaaga tgacttgcaa ggtgctattt atatttacaa tggccgtgca gatgggatct
     1501 cgtcaacctt ctcacagaga attgaaggac ttcagatcag caaatcgtta agtatgtttg
     1561 gacagtctat atcaggacaa attgatgcag ataataatgg ctatgtagat gtagcagttg
     1621 gtgcttttcg gtctgattct gctgtcttgc taaggacaag acctgtagta attgttgacg
     1681 cttctttaag ccaccctgag tcagtaaata gaacgaaatt tgactgtgtt gaaaatggat
     1741 ggccttctgt gtgcatagat ctaacacttt gtttctcata taagggcaag gaagttccag
     1801 gttacattgt tttgttttat aacatgagtt tggatgtgaa cagaaaggca gagtctccac
     1861 caagattcta tttctcttct aatggaactt ctgacgtgat tacaggaagc atacaggtgt
     1921 ccagcagaga agctaactgt agaacacatc aagcatttat gcggaaagat gtgcgggaca
     1981 tcctcacccc aattcagatt gaagctgctt accaccttgg tcctcatgtc atcagtaaac
     2041 gaagtacaga ggaattccca ccacttcagc caattcttca gcagaagaaa gaaaaagaca
     2101 taatgaaaaa aacaataaac tttgcaaggt tttgtgccca tgaaaattgt tctgctgatt
     2161 tacaggtttc tgcaaagatt gggtttttga agccccatga aaataaaaca tatcttgctg
     2221 ttgggagtat gaagacattg atgttgaatg tgtccttgtt taatgctgga gatgatgcat
     2281 atgaaacgac tctacatgtc aaactacccg tgggtcttta tttcattaag attttagagc
     2341 tggaagagaa gcaaataaac tgtgaagtca cagataactc tggcgtggta caacttgact
     2401 gcagtattgg ctatatatat gtagatcatc tctcaaggat agatattagc tttctcctgg
     2461 atgtgagctc actcagcaga gcggaagagg acctcagtat cacagtgcat gctacctgtg
     2521 aaaatgaaga ggaaatggac aatctaaagc acagcagagt gactgtagca atacctttaa
     2581 aatatgaggt taagctgact gttcatgggt ttgtaaaccc aacttcattt gtgtatggat
     2641 caaatgatga aaatgagcct gaaacgtgca tggtggagaa aatgaactta actttccatg
     2701 ttatcaacac tggcaatagt atggctccca atgttagtgt ggaaataatg gtaccaaatt
     2761 cttttagccc ccaaactgat aagctgttca acattttgga tgtccagact actactggag
     2821 aatgccactt tgaaaattat caaagagtgt gtgcattaga gcagcaaaag agtgcaatgc
     2881 agaccttgaa aggcatagtc cggttcttgt ccaagactga taagaggcta ttgtactgca
     2941 taaaagctga tccacattgt ttaaatttct tgtgtaattt tgggaaaatg gaaagtggaa
     3001 aagaagccag tgttcatatc caactggaag gccggccatc cattttagaa atggatgaga
     3061 cttcagcact caagtttgaa ataagagcaa caggttttcc agagccaaat ccaagagtaa
     3121 ttgaactaaa caaggatgag aatgttgcgc atgttctact ggaaggacta catcatcaaa
     3181 gacccaaacg ttatttcacc atagtgatta tttcaagtag cttgctactt ggacttattg
     3241 tacttctatt gatctcatat gttatgtgga aggctggctt ctttaaaaga caatacaaat
     3301 ctatcctaca agaagaaaac agaagagaca gttggagtta tatcaacagt aaaagcaatg
     3361 atgattaagg acttctttca aattgagaga atggaaaaca gactcaggtt gtagtaaaga
     3421 aatttaaaag acactgttta caagaaaaaa tgaattttgt ttggacttct tttactcatg
     3481 atcttgtgac atattatgtc ttcatgcaag gggaaaatct cagcaatgat tactctttga
     3541 gatagaagaa ctgcaaaggt aataatacag ccaaagataa tctctcagct tttaaatggg
     3601 tagagaaaca ctaaagcatt caatttattc aagaaaagta agcccttgaa gatatcttga
     3661 aatgaaagta taactgagtt aaattatact ggagaagtct tagacttgaa atactactta
     3721 ccatatgtgc ttgcctcagt aaaatgaacc ccactgggtg ggcagaggtt catttcaaat
     3781 acatctttga tacttgttca aaatatgttc tttaaaaata taatttttta gagagctgtt
     3841 cccaaatttt ctaacgagtg gaccattatc actttaaagc cctttattta taatacattt
     3901 cctacgggct gtgttccaac aaccattttt tttcagcaga ctatgaatat tatagtatta
     3961 taggccaaac tggcaaactt cagactgaac atgtacactg gtttgagctt agtgaaatta
     4021 cttctggata attatttttt tataattatg gatttcacca tctttctttc tgtatatata
     4081 catgtgtttt tatgtaggta tatatttacc attcttccta tctattcttc ctataacaca
     4141 cctttatcaa gcatacccag gagtaatctt caaatctttt gttatattct gaaacaaaag
     4201 attgtgagtg ttgcacttta cctgatacac gctgatttag aaaatacaga aaccatacct
     4261 cactaataac tttaaaatca aagctgtgca aagactaggg ggcctatact tcatatgtat
     4321 tatgtactat gtaaaatatt gactatcaca caactatttc cttggatgta attctttgtt
     4381 accctttaca agtataagtg ttaccttaca tggaaacgaa gaaacaaaat tcataaattt
     4441 aaattcataa atttagctga aagatactga ttcaatttgt atacagtgaa tataaatgag
     4501 acgacagcaa aattttcatg aaatgtaaaa tatttttata gtttgttcat actatatgag
     4561 gttctatttt aaatgacttt ctggatttta aaaaatttct ttaaatacaa tcatttttgt
     4621 aatatttatt ttatgcttat gatctagata attgcagaat atcattttat ctgactctgc
     4681 cttcataaga gagctgtggc cgaattttga acatctgtta tagggagtga tcaaattaga
     4741 aggcaatgtg gaaaaacaat tctgggaaag atttctttat atgaagtccc tgccactagc
     4801 cagccatcct aattgatgaa agttatctgt tcacaggcct gcagtgatgg tgaggaatgt
     4861 tctgagattt gcgaaggcat ttgagtagtg aaatgtaagc acaaaacctc ctgaacccag
     4921 agtgtgtata cacaggaata aactttatga catttatgta tttttaaaaa actttgtatc
     4981 gttataaaaa ggctagtcat tctttcagga gaacatctag gatcatagat gaaaaatcaa
     5041 gccccgattt agaactgtct tctccaggat ggtctctaag gaaatttaca tttggttctt
     5101 tcctactcag aactactcag aaacaactat atatttcagg ttatctgagc acagtgaaag
     5161 cagagtacta tggttgtcca acacaggcct ctcagataca aggggaacac aattacatat
     5221 tgggctagat tttgcccagt tcaaaatagt atttgttatc aacttacttt gttacttgta
     5281 tcatgaattt taaaacccta ccactttaag aagacaggga tgggttattc ttttttggca
     5341 ggtaggctat ataactatgt gattttgaaa tttaactgct ctggattagg gagcagtgaa
     5401 tcaaggcaga cttatgaaat ctgtattata tttgtaacag aatataggaa atttaacata
     5461 attgatgagc tcaaatcctg aaaaatgaaa gaatccaaat tatttcagaa ttatctaggt
     5521 taaatattga tgtattatga tggttgcaaa gtttttttgt gtgtccaata aacacattgt
     5581 aaaaaaaaga atttgaattg atatctaaaa acagaatttg aattgatatt tcatcttgac
     5641 ttttaaagcc ctagaggcta attgttagta acatcaattt ctattaggat atccgtttgg
     5701 ccacacagca ggaggttaga gcaatggagc attactgagt tcctccccct gtcagatcag
     5761 cagcagcatt agattctcat agaagtgcga accatatggt gaactggtat gtgagggatc
     5821 tagagtgcca tgttcctcaa gagaatctaa tgcctgatga tctgaggtgg aacagttcat
     5881 cctgaaacca ttcccccatc cacggaaaaa ttgtcttcca tgaaactggt cccaaaaagg
     5941 gtggggacca caggtttaaa gcatggccac atttctttat attaaaattc tagtttgtac
     6001 atttctttta gaaacaatta catgttactt tggaatcatt tcttccatgc ttcctccata
     6061 aagactgata agtcttggat gcaatctgta aagaaaatac attatttcat caacttattt
     6121 tgttgttttt cacatacacc taataagtat ggtacacaat gccaatgcca aatacaaatt
     6181 gataacaaac acagcattcc caacagagct gtaatctaga aaactgagaa ggtctgattg
     6241 ataaatcatc aacaacaata attgctctaa aacctcctta actgacttcc ttgattgtcc
     6301 aatgctctcc attacctctg taaaacagtc agttatgcct ctagaacacc catgtctagt
     6361 gggcacccct gcatgcttct tctaaccact gagtgtcaca atgcctacca agaatgcgtt
     6421 tgcaggttcc taaacctgtt tataccagtt gctatgtaaa attgttccca agggaagttg
     6481 aatgctctgt aaaggcctaa taaaagcaaa ttactgaaca aaacatgtta cagtaattat
     6541 gagtgagagg aaactaagat ggaaggataa aaatctaaca ctttactatt cagatggctc
     6601 cactaaaaga tttaagatct tgatccattt ttaaaaatcc aaaatggaag ttgtagacat
     6661 tatctgtagt ttatgcacaa caataaatta gaaagccaat gtagacacgc ataaccaaag
     6721 aaaatgcctt gggtctacat aacagttgaa taaatgtaaa gttgcttttt atttattgaa
     6781 a
//
