LOCUS       NM_001204890            4898 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens ISY1-RAB43 readthrough (ISY1-RAB43), mRNA.
ACCESSION   NM_001204890
VERSION     NM_001204890.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Prakash,T., Sharma,V.K., Adati,N., Ozawa,R., Kumar,N., Nishida,Y.,
            Fujikake,T., Takeda,T. and Taylor,T.D.
  TITLE     Expression of conjoined genes: another mechanism for gene
            regulation in eukaryotes
  JOURNAL   PLoS One 5 (10), e13284 (2010)
   PUBMED   20967262
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC108673.11 and AB032986.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001204890.1.
            
            Summary: This locus represents naturally occurring read-through
            transcription between the neighboring ISY1 (ISY1 splicing factor
            homolog) and RAB43 (RAB43, member RAS oncogene family) gene on
            chromosome 3. The read-through transcript encodes a protein that
            shares sequence identity with the upstream gene product, but its
            C-terminus is distinct due to a frameshift relative to the
            downstream gene. [provided by RefSeq, Mar 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB032986.1, SRR14038194.981817.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 57461, 339122
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AC108673.11        122777-122803       c
            28-1652             AB032986.1         1-1625
            1653-4898           AC108673.11        49312-52557         c
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.3"
     gene            1..4898
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /note="ISY1-RAB43 readthrough"
                     /db_xref="GeneID:100534599"
                     /db_xref="HGNC:HGNC:42969"
     exon            1..91
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..1084
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /note="Pre-mRNA-splicing factor ISY1 homolog"
                     /codon_start=1
                     /product="ISY1-RAB43 protein"
                     /protein_id="NP_001191819.1"
                     /db_xref="GeneID:100534599"
                     /db_xref="HGNC:HGNC:42969"
                     /translation="MARNAEKAMTALARFRQAQLEEGKVKERRPFLASECTELPKAEK
                     WRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKV
                     GPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYY
                     GYLDEDDGVIVPLEQEYEKKLRAELVEKWKAEREARLARGEKEEEEEEEEEINIYAVT
                     EEESDEEGSQEKGGDDSQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQS
                     EEARRLLGCRSGTRPARSGSAPSPRATTAVPMGPSLPTTSPRGAPSCRCLTGLRM"
     exon            92..114
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     exon            115..166
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     exon            167..232
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     exon            233..275
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     exon            276..388
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     exon            389..506
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     exon            507..629
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     exon            630..751
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     exon            752..838
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     exon            839..939
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1123
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..4898
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4876..4881
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /note="hexamer: ATTAAA"
     polyA_site      4898
                     /gene="ISY1-RAB43"
                     /gene_synonym="ISY1"
                     /note="major polyA site"
ORIGIN      
        1 agttcctgtg agcgtcggcg tcttctgggc ctgtggagtt tcttggacag gggccgcggg
       61 gctccaggac ggcgccctta gcgacaccat ggcccgaaat gcagaaaagg ccatgacggc
      121 cttagcaaga tttcgccagg ctcagctgga agagggaaaa gtgaaggaac gaagaccctt
      181 tctggcctca gaatgtactg aactgcctaa agctgagaag tggagacgac agatcattgg
      241 agagatctct aaaaaagtgg ctcagattca gaatgctggt ttaggtgaat ttcgaattcg
      301 tgacctgaat gatgaaatta acaagctgct aagggagaaa ggacactggg aggtccggat
      361 aaaggagctg ggaggtcctg attatggaaa agttggccct aaaatgctgg atcatgaagg
      421 aaaagaagtc ccaggaaacc gaggttacaa gtactttgga gcagcaaaag atttgcctgg
      481 tgttagagag ctgtttgaaa aagaacctct tcctcctccc agaaagacac gtgctgagct
      541 catgaaggca atcgattttg agtactatgg ttacctagat gaagatgatg gtgttattgt
      601 gcctttggaa caggaatatg aaaagaaact cagagccgag ttagtggaaa agtggaaagc
      661 agagagagag gctcggctgg caagaggaga aaaggaagag gaggaggaag aggaggaaga
      721 gatcaacatc tatgcagtca ccgaggagga gtcggacgag gaaggcagcc aggagaaagg
      781 aggggacgac agccagcaga agttcattgc tcacgtccct gttccctcgc agcaagagat
      841 tgaggaggca ctggtgcgaa ggaagaaaat ggaactcctc cagaagtatg caagcgagac
      901 cctgcaggcc caaagtgaag aagccagaag gctcctgggc tgcagatctg ggacacggcc
      961 ggccaggagc ggttccgcac catcacccag agctactacc gcagtgccaa tggggccatc
     1021 cttgcctacg acatcaccaa gaggagctcc ttcctgtcgg tgcctcactg gattgaggat
     1081 gtgaggaagt atgcgggctc caacattgtg cagctgctga tcgggaacaa gtcagacctc
     1141 agcgagcttc gggaggtctc cttggctgag gcacagagcc tggctgagca ctatgacatc
     1201 ctgtgtgcca ttgagacgtc tgccaaggac tcgagcaacg tggaggaggc cttcctgagg
     1261 gtggccacgg agctcatcat gcggcacggg ggccccttgt tcagcgagaa gagccccgac
     1321 cacatccagc tgaacagcaa ggacatcgga gaaggctggg gctgcgggtg ctgaccaggg
     1381 gccgggccgg cagactgggg gttccccacc tccttgctct ccccagcctg ccaagcccag
     1441 ccctccagag ccagccctcc tgggtaccgg caactacagc agccgggtga agctctggag
     1501 ctctgcatcc tgtggcctgg ctgcgggatg gaggctctcc ttgaggaagg ggaagcagga
     1561 taccctggcg ggccaccctg ccagccagca gctggccctc caccatcttc acattccaag
     1621 actggcctga acccgccggt gtgggccaca gtaggagggg ccagcagtcc tccctgtccc
     1681 atgcctgctg gctctgaccg tttcctctca tgggtttgct ttacctgtgg cagcaggttg
     1741 ctgctttcca gggatgtgtc cagtgccctg ggttcttccc ggtcatcctg gggctctgca
     1801 cagagcagca gagctagagc catgagaccc acagacttgg tgcctgagcg cagctccagg
     1861 gaagccccag ccccttccag gcggggaacc tgcttcctgc cttcactgcc cccgcagcac
     1921 tcacagacat ctccctgggg tctggctcac tgccttatca ggacctctga gttttctgac
     1981 tttctgtgta gcttctcatt ttcatcagca cagcatgggg tcacggccca cagctaggtg
     2041 gggtcacggc ccacagctag gtggggactg tctgctggct cagggcccag tgttttgcca
     2101 ctcaggttgc aaaattagct aatccttcag tgccagcact cagaagtctg gcaggtgggg
     2161 gaatgagatg agaaagtgga catttaaggt ccaagtagaa gcttgcatcc ttgaggctca
     2221 gggatgagag ggcaccgcaa ggcacccagc cttaccccac aagcaggaat cctgccagcc
     2281 gagcgtgggt cccagtctat ggaatcgagt ctcccagaca aaaaaggaga ggccctggcg
     2341 gtccttggcc tggcccattt tattccctgg acccttaact gcctcaccct gagctgggca
     2401 ccaaggttag aaggcaaaag tggggtaggg ggatgtggga agcagcattg tcctctgggc
     2461 atgtggctca gaggtatcac agtgacaaaa gagccagggc tagggctgga ggggccaggt
     2521 gatcttcctt agccaatccc agtccgtctg tgtcagccca gctctgcctc gcagccccct
     2581 cagcatgggt gcccccagaa gtaagcagct cttggaccca cgagcagggc tgcgtcggtt
     2641 tgtggtctag ctcccagggg caggccaggc atggctcagt gcagtccctg ggccagtgag
     2701 aggcctcccc catgcctccc tgccctctgc actaagcact ttccaggcag gcctggccat
     2761 agggcaagag caggccttac agcagtgcca cggtgaccca agaggcaggg ttggggcctg
     2821 gacagggttt cctctgctga gactgagaca agcagggctt gatgctggtg gtggagccag
     2881 catctcacct gccaggcttc cttttgggca gggaggctac ctgtggaatg gctgagcctg
     2941 gttctgcagg ggaggaaggc tgccaggagc tgaggggcta gcacctgcat tgaggggagt
     3001 ctgggtgcat cagattgggg ggcgccagag gcgaggctgc ctggctccct ttctcagcag
     3061 ctttgcttgt ggcaggggtg agagtggagc tgagtggggt ctgggctctg cccctgcgcc
     3121 tccactgcag tgcctgggct ccccttgcag ggcagacagt gcctggcact gaggggtgcc
     3181 cccagacccc gctgggactg gatgtgccgt gggcagtgcc ttcctccagc ctcacaacct
     3241 ttctgctggg gctgaagttt ctggggtcac atggtcagaa ggggctgagc tggtctttca
     3301 ctgcaggccc actctgaaag agaaatccca tgaagcctgt ccttgcagag cccctctgtt
     3361 tttgggggaa cagggagcat tcacagggtc ttgggaacaa aggcctgggc ctccccgttt
     3421 gaatttcagc cccagttcca gggcaagtcc cagccaccca agcatactta ccagtactgc
     3481 ccaccaccac ccactgccca cctctaagaa gcccggctct cctactctgc ttccaggctg
     3541 gggcctcaca ggtggggctg ttaccttcct cagggcttag ggagggaggg aggacatgga
     3601 cagggccttc caccctggct ccttcacagg gatggtagct gaccggtgat gggttcattt
     3661 gtcaccttgg actgtgagcc acttccactt ctcgcccccc aggcaggagc accctcactc
     3721 caagggcttg gcctggaaag gcaggctcac ctgggactca acgttccttc ccccgccgcc
     3781 actgtgcccc ccgtccctcc accccagaag ccctgacctc atttgggcac cttgctgtcc
     3841 tctgggcttc tcctctgccc aggtcaccct gtggcaggcc tgggccctca ggatgtttca
     3901 aaggctccac agctccaccg ctccacctgg cttcactcca gcaccctgtc cctggagcac
     3961 ctctgtcctg tctgggccaa cctggtctcc ctgccccatg ccctcccctg gctcttagca
     4021 acctgccctt ttattcatgc catttggggt cacagatgcc tagctggaca cagctcccag
     4081 aactggctcc accctggggc cttgccccta gtaccccctg gcaggagcaa cccaccccat
     4141 ggtcgaggtt cccctacccc tggatcctgg actgaagcca tttcctacag agaagcgttc
     4201 ccaccccctc tcaggcacaa aagccggtgt ccagggactc aagacctgta cctggggttg
     4261 caggggctcc tgccctgctg tatcccacca ggagggggcc ccactgccct tccggccctt
     4321 aaaactgggc tgggagagaa gacggggaag tgtgtgagac aaaacaaagc cggcttgttt
     4381 cctccccttg gcaggctaag ctctcccggc agcccgggct ctggggcgga aggagaacag
     4441 tgtcatctct tcctttgttg ataaattgcc aaagagtggg aacaggtggg gtcaggggcc
     4501 agagtgcagg ggagggggca atggtggagg tggcgggggg aagcagaggg aggtgggctg
     4561 aggcctcggt ggggccagtg cgacgctggc ttaaggagct ggaggggttc ctaatacaca
     4621 tttaattcag tttctcttcc ctaagaggct gccggagttg gggcctcctc cagcagagac
     4681 cctcggaccc ctgcagggcc tggacttggg gtgaacaggg cttcagtcag cgcaagtatt
     4741 ccatttgcat ttggtaattt ttcatgccac ctatttatga atatataaat ctttatacca
     4801 aatctatttt ttaaaacatg gaaaagttgc ctttatggaa acttggcaga gccagagtgt
     4861 acacattcct aaaccattaa acagatttct ataacaag
//
