LOCUS       NM_020701               3612 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ISY1 splicing factor homolog (ISY1), transcript
            variant 2, mRNA.
ACCESSION   NM_020701 XM_939909
VERSION     NM_020701.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 3612)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3612)
  AUTHORS   Jaiswal,A.S., Williamson,E.A., Srinivasan,G., Kong,K.,
            Lomelino,C.L., McKenna,R., Walter,C., Sung,P., Narayan,S. and
            Hromas,R.
  TITLE     The splicing component ISY1 regulates APE1 in base excision repair
  JOURNAL   DNA Repair (Amst) 86, 102769 (2020)
   PUBMED   31887540
  REMARK    GeneRIF: The splicing component ISY1 regulates APE1 in base
            excision repair.
REFERENCE   5  (bases 1 to 3612)
  AUTHORS   Zhan,X., Yan,C., Zhang,X., Lei,J. and Shi,Y.
  TITLE     Structure of a human catalytic step I spliceosome
  JOURNAL   Science 359 (6375), 537-545 (2018)
   PUBMED   29301961
REFERENCE   6  (bases 1 to 3612)
  AUTHORS   De,I., Bessonov,S., Hofele,R., dos Santos,K., Will,C.L., Urlaub,H.,
            Luhrmann,R. and Pena,V.
  TITLE     The RNA helicase Aquarius exhibits structural adaptations mediating
            its recruitment to spliceosomes
  JOURNAL   Nat Struct Mol Biol 22 (2), 138-144 (2015)
   PUBMED   25599396
REFERENCE   7  (bases 1 to 3612)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   8  (bases 1 to 3612)
  AUTHORS   Hegele,A., Kamburov,A., Grossmann,A., Sourlis,C., Wowro,S.,
            Weimann,M., Will,C.L., Pena,V., Luhrmann,R. and Stelzl,U.
  TITLE     Dynamic protein-protein interaction wiring of the human spliceosome
  JOURNAL   Mol Cell 45 (4), 567-580 (2012)
   PUBMED   22365833
REFERENCE   9  (bases 1 to 3612)
  AUTHORS   Villa,T. and Guthrie,C.
  TITLE     The Isy1p component of the NineTeen complex interacts with the
            ATPase Prp16p to regulate the fidelity of pre-mRNA splicing
  JOURNAL   Genes Dev 19 (16), 1894-1904 (2005)
   PUBMED   16103217
  REMARK    GeneRIF: Describes the function of yeast Isy1p in pre-mRNA
            splicing.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC019849.1, AC108673.11 and AK024035.1.
            
            On Jun 2, 2019 this sequence version replaced NM_020701.3.
            
            Transcript Variant: This variant (2) lacks an exon in the coding
            region but maintains the reading frame, compared to variant 1. This
            results in a shorter protein (isoform 2), compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.3295726.1, BC019849.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000393295.8/ ENSP00000376973.4
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1635              BC019849.1         124-1758
            1636-1651           AC108673.11        91116-91131         c
            1652-3611           AK024035.1         1-1960
            3612-3612           AC108673.11        89155-89155         c
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.3"
     gene            1..3612
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /note="ISY1 splicing factor homolog"
                     /db_xref="GeneID:57461"
                     /db_xref="HGNC:HGNC:29201"
                     /db_xref="MIM:612764"
     exon            1..91
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..946
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /note="isoform 2 is encoded by transcript variant 2;
                     functional spliceosome-associated protein 33;
                     pre-mRNA-splicing factor ISY1 homolog"
                     /codon_start=1
                     /product="pre-mRNA-splicing factor ISY1 homolog isoform 2"
                     /protein_id="NP_065752.1"
                     /db_xref="CCDS:CCDS43149.1"
                     /db_xref="GeneID:57461"
                     /db_xref="HGNC:HGNC:29201"
                     /db_xref="MIM:612764"
                     /translation="MARNAEKAMTALARFRQAQLEEGKVKERRPFLASECTELPKAEK
                     WRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKV
                     GPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYY
                     GYLDEDDGVIVPLEQEYEKKLRAELVEKWKAEREARLARGEKEEEEEEEEEINIYAVT
                     EEESDEEGSQEKGGDDSQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQS
                     EEARRLLGY"
     exon            92..114
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /inference="alignment:Splign:2.1.0"
     exon            115..166
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /inference="alignment:Splign:2.1.0"
     exon            167..232
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /inference="alignment:Splign:2.1.0"
     exon            233..275
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /inference="alignment:Splign:2.1.0"
     exon            276..388
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /inference="alignment:Splign:2.1.0"
     exon            389..506
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /inference="alignment:Splign:2.1.0"
     exon            507..629
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /inference="alignment:Splign:2.1.0"
     exon            630..751
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /inference="alignment:Splign:2.1.0"
     exon            752..838
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /inference="alignment:Splign:2.1.0"
     exon            839..3612
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1198
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /note="major polyA site"
     regulatory      1818..1823
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /note="hexamer: ATTAAA"
     polyA_site      1844
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /note="major polyA site"
     regulatory      3585..3590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
                     /note="hexamer: AATAAA"
     polyA_site      3612
                     /gene="ISY1"
                     /gene_synonym="FSAP33"
ORIGIN      
        1 agttcctgtg agcgtcggcg tcttctgggc ctgtggagtt tcttggacag gggccgcggg
       61 gctccaggac ggcgccctta gcgacaccat ggcccgaaat gcagaaaagg ccatgacggc
      121 cttagcaaga tttcgccagg ctcagctgga agagggaaaa gtgaaggaac gaagaccctt
      181 tctggcctca gaatgtactg aactgcctaa agctgagaag tggagacgac agatcattgg
      241 agagatctct aaaaaagtgg ctcagattca gaatgctggt ttaggtgaat ttcgaattcg
      301 tgacctgaat gatgaaatta acaagctgct aagggagaaa ggacactggg aggtccggat
      361 aaaggagctg ggaggtcctg attatggaaa agttggccct aaaatgctgg atcatgaagg
      421 aaaagaagtc ccaggaaacc gaggttacaa gtactttgga gcagcaaaag atttgcctgg
      481 tgttagagag ctgtttgaaa aagaacctct tcctcctccc agaaagacac gtgctgagct
      541 catgaaggca atcgattttg agtactatgg ttacctagat gaagatgatg gtgttattgt
      601 gcctttggaa caggaatatg aaaagaaact cagagccgag ttagtggaaa agtggaaagc
      661 agagagagag gctcggctgg caagaggaga aaaggaagag gaggaggaag aggaggaaga
      721 gatcaacatc tatgcagtca ccgaggagga gtcggacgag gaaggcagcc aggagaaagg
      781 aggggacgac agccagcaga agttcattgc tcacgtccct gttccctcgc agcaagagat
      841 tgaggaggca ctggtgcgaa ggaagaaaat ggaactcctc cagaagtatg caagcgagac
      901 cctgcaggcc caaagtgaag aagccagaag gctcctgggg tattaggacc cagctggggc
      961 tctccttgga gttcttccat cccccagtgg tacctcagga cccagggctg cagacacagg
     1021 ctggtgctgc aagggctcct gccccattct cagccttcct tccctctcct tgtctcatgt
     1081 tgaccggagg gtaggggtct gtccctggtc ttcctggtag gttttgtaca catattttgc
     1141 tactgtgtgg atccatttat ttttattgtg gagtgtatac aacaggttgc gaactggctg
     1201 cctgtgtctt attttgactt gcactgccat tttgagggga gaagaatcaa ttagtggcaa
     1261 acatttaaaa atgcaatttt ttgcagacca aagtataatt ttaaaaaatg caaattttct
     1321 aaaagacaca tctcttgaaa aatgagatga tgtggccagg cgcagtggct cacgcctgta
     1381 accccagcac tttgggaggc cgaggcgggc gggtcacgag gtcaagagat ggagaccatc
     1441 ctggccaaca tggtgaaacc ccatgtctac taaaaataca aaaaaattag ctgggcgtac
     1501 tggcatgcac ctgtagtccc agctgcttgg gaggctgagg caggagaatc acttgaaccc
     1561 gagaggtgga ggttgaagtg agccaagatc gtgccattgc actccagcct ggcgacagag
     1621 tgagactctg tcccaaaaaa aaaaaaaaaa aaagatgcac gcaacccaca agtcaatgac
     1681 caggtccctc tgtgttggtt gctgctctgg gctctccgtt agcccccctc cacctgctcc
     1741 actcgctcct gcctgggtgg caggaatttg agtttgctat tcctgtttta taacctgttc
     1801 tgcagaaacc tatttttatt aaagattttt ataagaaagg gaaacctcca tgtcttcagc
     1861 ttgttccctg ggccaacggc tctgaggtga cagtggcatt aggatgtgac cagtcactaa
     1921 ggggcactgc cttcctgcag gggagtccct gaagccccag ccccaagtgt ggcaaagaca
     1981 ggaccagatc ccaggagtct aggtgggagt gagccgaccc accatggcgt ccgtggccac
     2041 agctgtagac agggctcctt gcagtgggct gcaccgtggc ctgctgaaag cacacagtgg
     2101 acacgagctg agtccagagc ttcctctgaa ctggggctta gctctccagt ggggaaccat
     2161 ggtcagcggc tgtagcccct gggcaagatg gtgcctggct tcctgccctc tgcagggtca
     2221 tgggagtgtc tgggaactca gggtcagggc cagatggctg ttcctggggc ctggggctcc
     2281 agctcaggct gtttgggaat aggaggaact cttggcttgg agaagcccat cacacagcgg
     2341 gaggcctggg atggttggtc tgcggggagc agctcagact gggggtggca gagccgtgtg
     2401 tggctgtgtg tggccaagtc gggagagtgg ccagtgctgg gccctctcta aggagcccga
     2461 gggccacaag gactgtggaa aaggtctgag tgagggagtc gggagctagt ggctaaggga
     2521 cttgcttgag gacgctccgt gcggccacaa ggagaggccc aggcaggacc aggtcaggag
     2581 gaggctgggg cagaggccct ggcaggaggt gggtgggctt ggtggggatg ggagtagcca
     2641 gggtgtaggt gaggatgggg aaggggcccc cctgcctcct ggcttccctg agggctgggc
     2701 tggccaatcc cagccctcag tcaagcccgg aggcaaatgt gtgcagggag gagaggtgtc
     2761 caggttgaag ctttgggagc ccttggaggc agcaaggcag actcgcctct gggacccagc
     2821 acccagcttg ggcctgcagg tggggactca ggagtgaata tcgtgggggc tttgagcaag
     2881 accctctggg cccagcctca ccttccatac tttgaagtca aggagtggac ctgtgctgtc
     2941 tcagtttgct ctggggtctg ggcagtcctg tggtttgggc agaggcctca gagggcccta
     3001 gggacagact gacccaggtc tgagcctctt gggagcgtgc ggatggccgg cctcagacag
     3061 gcctggttgt gggtttgtct tccaaggggc acacgcgcac tctggtgctt cctggcattc
     3121 agaagagaat ggggaatcca gacatcaggc cttccttctc cctgggaagg atggcaggtg
     3181 cttggagcca ggcctgtctt gagagccagg gctctgggtc ttcctcctcc agtgccctgt
     3241 tccacgcctt cagtgcacaa agcactcccc tcctgggatc tcttcaggga tccccgggat
     3301 gcaaactggc cagggattag aattccacat ttgagcaaac agactgagag tggagaagca
     3361 cccagctgga catcctctag gtgttcctgg cagaggccct tgctacttgt ctgtgcccaa
     3421 aggccctaga gggtcctaga tgtgggacaa ggtctcagca gggctccagt cccaggcctg
     3481 gaaacagctg ggggtggggg aagctggctg ggtgggtgtg gttatctttt cccagcttgt
     3541 ggctccgagg tgacagaacc aatctcagga ggctttgttt ggttaataaa atctactgaa
     3601 tggtttattt ta
//
