LOCUS       NM_001303508            3073 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens intestine specific homeobox (ISX), mRNA.
ACCESSION   NM_001303508
VERSION     NM_001303508.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Bui,A.Q., Gunathilake,M., Lee,J., Oh,J.H., Chang,H.J., Sohn,D.K.,
            Shin,A. and Kim,J.
  TITLE     Interaction between retinol intake and ISX rs5755368 polymorphism
            in colorectal cancer risk: a case-control study in a Korean
            population
  JOURNAL   Sci Rep 13 (1), 10187 (2023)
   PUBMED   37349365
  REMARK    GeneRIF: Interaction between retinol intake and ISX rs5755368
            polymorphism in colorectal cancer risk: a case-control study in a
            Korean population.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Wang,L.T., Liu,K.Y., Chiou,S.S., Huang,S.K., Hsu,S.H. and Wang,S.N.
  TITLE     Phosphorylation of intestine-specific homeobox by ERK1 modulates
            oncogenic activity and sorafenib resistance
  JOURNAL   Cancer Lett 520, 160-171 (2021)
   PUBMED   34265398
  REMARK    GeneRIF: Phosphorylation of intestine-specific homeobox by ERK1
            modulates oncogenic activity and sorafenib resistance.
REFERENCE   3  (bases 1 to 3073)
  AUTHORS   Chuang,K.T., Wang,S.N., Hsu,S.H. and Wang,L.T.
  TITLE     Impact of bromodomain-containing protein 4 (BRD4) and
            intestine-specific homeobox (ISX) expression on the prognosis of
            patients with hepatocellular carcinoma' for better clarity
  JOURNAL   Cancer Med 10 (16), 5545-5556 (2021)
   PUBMED   34173348
  REMARK    GeneRIF: Impact of bromodomain-containing protein 4 (BRD4) and
            intestine-specific homeobox (ISX) expression on the prognosis of
            patients with hepatocellular carcinoma' for better clarity.
REFERENCE   4  (bases 1 to 3073)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3073)
  AUTHORS   Wang,L.T., Chiou,S.S., Chai,C.Y., Hsi,E., Yokoyama,K.K., Wang,S.N.,
            Huang,S.K. and Hsu,S.H.
  TITLE     Intestine-Specific Homeobox Gene ISX Integrates IL6 Signaling,
            Tryptophan Catabolism, and Immune Suppression
  JOURNAL   Cancer Res 77 (15), 4065-4077 (2017)
   PUBMED   28625979
  REMARK    GeneRIF: Overall, our results identified a feed-forward mechanism
            of immune suppression in hepatocellular carcinoma organized by ISX,
            which involves kynurenine-AHR signaling and PD-L1, offering
            insights into immune escape by hepatocellular carcinoma
REFERENCE   6  (bases 1 to 3073)
  AUTHORS   Sue,S., Shibata,W., Kameta,E., Sato,T., Ishii,Y., Kaneko,H.,
            Miwa,H., Sasaki,T., Tamura,T., Kondo,M. and Maeda,S.
  TITLE     Intestine-specific homeobox (ISX) induces intestinal metaplasia and
            cell proliferation to contribute to gastric carcinogenesis
  JOURNAL   J Gastroenterol 51 (10), 949-960 (2016)
   PUBMED   26872890
  REMARK    GeneRIF: ISX expression induced by H. pylori infection may lead to
            intestinal metaplasia and hyperproliferation of gastric mucosa
            through CDX1/2 and cyclinD1 expression, contributing to gastric
            carcinogenesis.
REFERENCE   7  (bases 1 to 3073)
  AUTHORS   Lobo,G.P., Amengual,J., Baus,D., Shivdasani,R.A., Taylor,D. and von
            Lintig,J.
  TITLE     Genetics and diet regulate vitamin A production via the homeobox
            transcription factor ISX
  JOURNAL   J Biol Chem 288 (13), 9017-9027 (2013)
   PUBMED   23393141
  REMARK    GeneRIF: Genetics and diet regulate vitamin A production via the
            homeobox transcription factor ISX
REFERENCE   8  (bases 1 to 3073)
  AUTHORS   Hsu,S.H., Wang,L.T., Lee,K.T., Chen,Y.L., Liu,K.Y., Suen,J.L.,
            Chai,C.Y. and Wang,S.N.
  TITLE     Proinflammatory homeobox gene, ISX, regulates tumor growth and
            survival in hepatocellular carcinoma
  JOURNAL   Cancer Res 73 (2), 508-518 (2013)
   PUBMED   23221382
  REMARK    GeneRIF: our results highlight ISX as an important regulator in
            hepatoma progression with significant potential as a prognostic and
            therapeutic target in HCCs.
REFERENCE   9  (bases 1 to 3073)
  AUTHORS   Vaquerizas,J.M., Kummerfeld,S.K., Teichmann,S.A. and Luscombe,N.M.
  TITLE     A census of human transcription factors: function, expression and
            evolution
  JOURNAL   Nat Rev Genet 10 (4), 252-263 (2009)
   PUBMED   19274049
  REMARK    Review article
REFERENCE   10 (bases 1 to 3073)
  AUTHORS   Seino,Y., Miki,T., Kiyonari,H., Abe,T., Fujimoto,W., Kimura,K.,
            Takeuchi,A., Takahashi,Y., Oiso,Y., Iwanaga,T. and Seino,S.
  TITLE     Isx participates in the maintenance of vitamin A metabolism by
            regulation of beta-carotene 15,15'-monooxygenase (Bcmo1) expression
  JOURNAL   J Biol Chem 283 (8), 4905-4911 (2008)
   PUBMED   18093975
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CR749372.1, Z83853.2, AL024494.1 and AK025181.1.
            
            On May 17, 2019 this sequence version replaced NM_001303508.1.
            
            Summary: Homeobox genes encode DNA-binding proteins, many of which
            are thought to be involved in early embryonic development. Homeobox
            genes encode a DNA-binding domain of 60 to 63 amino acids referred
            to as the homeodomain. This gene is a member of the RAXLX homeobox
            gene family. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR749372.1, SRR11853560.19573.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000404699.7/ ENSP00000386037.1
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-546               CR749372.1         24-569
            547-547             Z83853.2           5836-5836           c
            548-1468            CR749372.1         571-1491
            1469-1469           AL024494.1         13055-13055
            1470-3073           AK025181.1         1781-3384
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.3"
     gene            1..3073
                     /gene="ISX"
                     /gene_synonym="Pix-1; RAXLX"
                     /note="intestine specific homeobox"
                     /db_xref="GeneID:91464"
                     /db_xref="HGNC:HGNC:28084"
                     /db_xref="MIM:612019"
     exon            1..305
                     /gene="ISX"
                     /gene_synonym="Pix-1; RAXLX"
                     /inference="alignment:Splign:2.1.0"
     exon            306..870
                     /gene="ISX"
                     /gene_synonym="Pix-1; RAXLX"
                     /inference="alignment:Splign:2.1.0"
     CDS             642..1379
                     /gene="ISX"
                     /gene_synonym="Pix-1; RAXLX"
                     /note="pancreas-intestine homeodomain transcription
                     factor; RAX-like homeobox"
                     /codon_start=1
                     /product="intestine-specific homeobox"
                     /protein_id="NP_001290437.1"
                     /db_xref="CCDS:CCDS33640.1"
                     /db_xref="GeneID:91464"
                     /db_xref="HGNC:HGNC:28084"
                     /db_xref="MIM:612019"
                     /translation="MCAEVGPALCRGMERNSLGCCEAPKKLSLSFSIEAILKRPARRS
                     DMDRPEGPGEEGPGEAAASGSGLEKPPKDQPQEGRKSKRRVRTTFTTEQLHELEKIFH
                     FTHYPDVHIRSQLAARINLPEARVQIWFQNQRAKWRKQEKIGNLGAPQQLSEASVALP
                     TNLDVAGPTWTSTALRRLAPPTSCCPSAQDQLASAWFPAWITLLPAHPWETQPVPGLP
                     IHQTCIPVLCILPPPHPKWGSICATST"
     exon            871..1022
                     /gene="ISX"
                     /gene_synonym="Pix-1; RAXLX"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1139
                     /gene="ISX"
                     /gene_synonym="Pix-1; RAXLX"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..3073
                     /gene="ISX"
                     /gene_synonym="Pix-1; RAXLX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3047..3052
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ISX"
                     /gene_synonym="Pix-1; RAXLX"
                     /note="hexamer: AATAAA"
     polyA_site      3073
                     /gene="ISX"
                     /gene_synonym="Pix-1; RAXLX"
                     /note="major polyA site"
ORIGIN      
        1 agtcagagag aaagggcctc ttcagtctgt ctcaggagac tgggagaaac agcataaagg
       61 accccacaag gaagggagag gtaccctggg tcaggcgctt gtggagagag ggcttcgcat
      121 gtaaagtgac gtcagggaaa atagaacaga aaaaaagcca gggccagccc agaggcacct
      181 gagaagaatc agacccacag ctcagcccag ccctggcaca gagaagagac aggcctggca
      241 gcacccaggg accccctttc ctcagcctcc acctgcagga cagcaggagc actgatgcgc
      301 tgaagggatc ctggcctaac ctggtgattg tgcaggcaac tgtgtccgag aagacccttc
      361 tctggaagat tgaaccccaa ttcagccatg gtgactcctt tgatgtcaaa ctggtaaggg
      421 ctgagccgtg ggcacaggat accactcctt ccagctcttc tgctgtgacc tgcccatgga
      481 agtccctgtg gacacgaaat cctgtttgga tcatctaact ggaggctctc tgttcttcac
      541 ctccacgcgc cctcttgacc ccaggaggtt caggggagga agtacgccac tctccactgg
      601 caccctcctt ggcctacaca gagtcacccc tgagcccctc aatgtgtgct gaggtgggcc
      661 ctgctctctg caggggtatg gagagaaata gcttggggtg ctgtgaggcc ccgaagaagc
      721 tgagcctgtc cttctccatt gaggcgatcc taaagaggcc tgccaggagg agtgatatgg
      781 acagaccaga agggccaggt gaagagggcc ccggagaagc tgcggcctca ggctctgggc
      841 tagaaaagcc tccaaaggac cagccccagg aaggaaggaa gagcaagcgg agggttcgta
      901 ccaccttcac cactgagcag ctgcatgagc tggagaagat cttccacttt acccactacc
      961 cagacgttca catccgcagc cagctggcag ccaggatcaa cctcccagaa gctcgggtgc
     1021 agatctggtt ccagaatcag cgagccaagt ggcggaagca ggagaagatt ggcaacctgg
     1081 gggctccaca gcagctgagt gaagccagtg tggccctgcc cacaaatctg gatgtggctg
     1141 ggcccacgtg gacatccact gctctgcgca ggctggctcc tcccacgagc tgttgtccat
     1201 cggctcaaga tcagctggcc tctgcctggt tccctgcctg gatcaccctc ctcccagcgc
     1261 acccatggga aacacagcct gtcccaggtc ttcccatcca tcaaacttgc atccctgtgc
     1321 tatgcatcct tccacctcca caccccaaat ggggcagcat ctgtgctact tcaacataga
     1381 gattggacat gctctcccca aatgagccac tttcctctcc aggtgaaggc aggtagcaga
     1441 tgtgccctgg gcctctgggg aaatcgattt cacaatccaa aaatggccca cagcccagga
     1501 agctaccctg aacatgccag ttggaaggct gcaccagact caaaagcaaa ctaaacaata
     1561 aaggacagct ctcttctctc ctggctaaag ctgctctcct ggttcagaag acaggctgga
     1621 tgagatctca ggccgagctc tgaaataggg aggtaatcct ccagcacctg tgtttcctct
     1681 aacttgctgt gtgacctcca gccggtcact caccctctct ggacctcatc tgtaagagga
     1741 gccagctgga taagatgatt tctgaagacg cttccatggt gggcactgag gcacagagga
     1801 ggccaaggag aggttgtttg ttcatgcatg cattcatccg tgacacatga gtacctactg
     1861 aggactccat aaacagaacg ggatacagag ataaacaatt tgggttctgt ccacgtttgt
     1921 caaaaggtgg tgctggccca cctctgaaag cagaacactt gctcaacaac cttgctgttg
     1981 gcccaagtct aacacattct ttatgactgt gagcatctca gagtgagaga aaaatgtaga
     2041 aagtttttta aattctaaac aggatttagt gtctttagtt atcttgctgg atgggaaagg
     2101 gatgttgtca tttctggcac aaatgaaaag taggacggaa agctcctttc attcagttta
     2161 tctttccagg atatatgaaa agggaccagc tggaagacta gcctcactct gtcctcgaaa
     2221 gcctgagctt tcattcaact ccctatttcc atgcaaagac gctgggcaaa ccacatgttc
     2281 tgtctgagcc tcagttttcc tatccataaa atgaaggtag ccaggcctgc ctcaaagagc
     2341 attcaggagg ctctgagagg acatgagagt attttgcaaa gtgagggcaa ggcccagtgt
     2401 ggagtgatat tgttattcca agattccact gcaaaagtgg ctgctttgga tgccagccca
     2461 ggatgagtag ttcctgttct cagggaggtc atccgctgag catcccttct gcacagatgt
     2521 ctctgattct tgtccttgca ggtggaggac agggcctgct cccctaagct gggaagcctg
     2581 gaatgacctc ttgcacaagc ctaaattcca ggaatcttcc ccaaatccca gatcctctgc
     2641 aatctacctg cacccctgac ccacccagga gttggaccgg gagttgggaa gcctaggtct
     2701 tagtcctaca ctccttctaa tttgctgtgt aaccttacca ttaatctctc tgggtctcag
     2761 ttttctcatc tgtattggag gtagcagtgc tagctctgcc ttcaggcatg caatatgcca
     2821 gaactacaga caacagccca caggatgcaa aagtgctttg ccatcttaaa aatgccagat
     2881 cactcagagc ctatgaatgt ggatatcaac accaggtctc tagcaccgct ggatgaaagg
     2941 agaaggctag aggctgaggg aggaaagagc agttaacaaa caaaggcagt agctcatcac
     3001 ttgggtagca ggtacccatt ttaggaccct acactcaaat gtgcaaaata aaatttctat
     3061 cattttgcta taa
//
