LOCUS       NM_001270976            4154 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens IST1 factor associated with ESCRT-III (IST1),
            transcript variant 3, mRNA.
ACCESSION   NM_001270976
VERSION     NM_001270976.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Chen,H., Cai,Z., Dong,X., Chen,W., Cao,C., Zheng,S., Wu,J.,
            Zhong,D., Cheng,S., Gao,Y. and Shou,J.
  TITLE     Cigarette smoking-related OLC1 overexpression associated with poor
            prognosis in bladder urothelial carcinoma
  JOURNAL   Life Sci 351, 122821 (2024)
   PUBMED   38880167
  REMARK    GeneRIF: Cigarette smoking-related OLC1 overexpression associated
            with poor prognosis in bladder urothelial carcinoma.
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Cada,A.K., Pavlin,M.R., Castillo,J.P., Tong,A.B., Larsen,K.P.,
            Ren,X., Yokom,A.L., Tsai,F.C., Shiah,J.V., Bassereau,P.M.,
            Bustamante,C.J. and Hurley,J.H.
  TITLE     Friction-driven membrane scission by the human ESCRT-III proteins
            CHMP1B and IST1
  JOURNAL   Proc Natl Acad Sci U S A 119 (29), e2204536119 (2022)
   PUBMED   35858336
  REMARK    GeneRIF: Friction-driven membrane scission by the human ESCRT-III
            proteins CHMP1B and IST1.
REFERENCE   3  (bases 1 to 4154)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 4154)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4154)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   6  (bases 1 to 4154)
  AUTHORS   Agromayor,M., Carlton,J.G., Phelan,J.P., Matthews,D.R.,
            Carlin,L.M., Ameer-Beg,S., Bowers,K. and Martin-Serrano,J.
  TITLE     Essential role of hIST1 in cytokinesis
  JOURNAL   Mol Biol Cell 20 (5), 1374-1387 (2009)
   PUBMED   19129480
  REMARK    GeneRIF: hIST1 is essential for cytokinesis in mammalian cells.
REFERENCE   7  (bases 1 to 4154)
  AUTHORS   Bajorek,M., Morita,E., Skalicky,J.J., Morham,S.G., Babst,M. and
            Sundquist,W.I.
  TITLE     Biochemical analyses of human IST1 and its function in cytokinesis
  JOURNAL   Mol Biol Cell 20 (5), 1360-1373 (2009)
   PUBMED   19129479
  REMARK    GeneRIF: IST1 and CHMP1 act together to recruit and modulate
            specific VPS4 activities required during the final stages of cell
            division.
REFERENCE   8  (bases 1 to 4154)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   9  (bases 1 to 4154)
  AUTHORS   Yuan,J., Ma,J., Zheng,H., Shi,T., Sun,W., Zhang,Q., Lin,D.,
            Zhang,K., He,J., Mao,Y., Gao,X., Gao,P., Han,N., Fu,G., Xiao,T.,
            Gao,Y., Ma,D. and Cheng,S.
  TITLE     Overexpression of OLC1, cigarette smoke, and human lung
            tumorigenesis
  JOURNAL   J Natl Cancer Inst 100 (22), 1592-1605 (2008)
   PUBMED   19001599
  REMARK    GeneRIF: OLC1 (KIAA0174) is a candidate oncogene in lung cancer
            whose expression may be regulated by exposure to cigarette smoke.
REFERENCE   10 (bases 1 to 4154)
  AUTHORS   Kaye,F.J.
  TITLE     Defining a candidate lung cancer gene
  JOURNAL   J Natl Cancer Inst 100 (22), 1564-1565 (2008)
   PUBMED   19001597
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA332434.1, AK293040.1 and
            AC009127.10.
            
            Summary: This gene encodes a protein with MIT-interacting motifs
            that interacts with components of endosomal sorting complexes
            required for transport (ESCRT). ESCRT functions in vesicle budding,
            such as that which occurs during membrane abscission in
            cytokinesis. There is a pseudogene for this gene on chromosome 19.
            Alternatively spliced transcript variants encoding multiple
            isoforms have been observed for this gene. [provided by RefSeq, Aug
            2012].
            
            Transcript Variant: This variant (3) differs in the 5' UTR and
            initiates translation at an alternate in-frame start codon,
            compared to variant 1. The encoded isoform (c) has a longer
            N-terminus, compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK293040.1 [ECO:0000332]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                DA332434.1         1-12
            13-1177             AK293040.1         1-1165
            1178-4154           AC009127.10        81745-84721         c
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.2"
     gene            1..4154
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /note="IST1 factor associated with ESCRT-III"
                     /db_xref="GeneID:9798"
                     /db_xref="HGNC:HGNC:28977"
                     /db_xref="MIM:616434"
     exon            1..74
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            75..212
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             189..1328
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /note="isoform c is encoded by transcript variant 3; IST1
                     homolog; charged multivesicular body protein 8; putative
                     MAPK-activating protein PM28; overexpressed in lung cancer
                     1; increased sodium tolerance 1 homolog; IST1, ESCRT-III
                     associated factor; IST1, endosomal sorting complex
                     required for transport-III component"
                     /codon_start=1
                     /product="IST1 homolog isoform c"
                     /protein_id="NP_001257905.1"
                     /db_xref="CCDS:CCDS59271.1"
                     /db_xref="GeneID:9798"
                     /db_xref="HGNC:HGNC:28977"
                     /db_xref="MIM:616434"
                     /translation="MVFKLKTKEEQHSMLGSGFKAERLRVNLRLVINRLKLLEKKKTE
                     LAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSM
                     KELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDR
                     LMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVK
                     KGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGT
                     YQAFPNIHPPQIPATPPSYESVDDINADKNISSAQIVGPGPKPEASAKLPSRPADNYD
                     NFVLPELPSVPDTLPTASAGASTSASEDIDFDDLSRRFEELKKKT"
     exon            213..315
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            316..496
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            497..584
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            585..668
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            669..779
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            780..986
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1079
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1128
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..4154
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2500..2505
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /note="hexamer: AATAAA"
     polyA_site      2520
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /note="major polyA site"
     regulatory      4125..4130
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /note="hexamer: AATAAA"
     polyA_site      4154
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
ORIGIN      
        1 atgcgtaatt tcctctttag tagagacggg gttcctctgt gttggccagg ctggtctcga
       61 attaccgact tcagcgatgc ggtttcacta tggtgtccag gctggtctgc aactcccggg
      121 ctcaagcgat cctcccgccc cgcctctcaa agtgctggga aggtcccttc caggcttaag
      181 cagcgataat ggttttcaag ttaaaaacaa aggaggaaca gcacagcatg ctgggctctg
      241 gatttaaagc tgagcgctta agagtgaatt tgagattagt cataaatcgc cttaaactat
      301 tggagaaaaa gaaaacggaa ctggcccaga aagcaaggaa ggagattgct gactatctgg
      361 ctgctgggaa agatgaacga gctcggatcc gtgtggagca cattatccgg gaagactacc
      421 tcgtggaggc catggagatc ctggagctgt actgtgacct gctgctggct cggtttggcc
      481 ttatccagtc tatgaaggaa ctagattctg gtctggctga atctgtgtct acattgatct
      541 gggctgctcc tcgactccag tcagaagtgg ctgagttgaa aatagttgct gatcagctct
      601 gtgccaagta tagcaaggaa tatggcaagc tatgtaggac caaccagatt ggaactgtga
      661 atgacaggct aatgcacaag ctgagtgtgg aagccccacc caaaatcctg gtggagagat
      721 acctgattga aattgcaaag aattacaacg taccctatga acctgactct gtggtcatgg
      781 cagaagctcc tcctggggta gagacagatc ttattgatgt tggattcaca gatgatgtga
      841 agaaaggagg ccctggaaga ggagggagtg gtggcttcac agcaccagtt ggtggacctg
      901 atggaacggt gccaatgccc atgcccatgc ccatgcctat gccatctgca aatacgcctt
      961 tctcatatcc actgccaaag ggaccatcag atttcaatgg actgccaatg gggacttatc
     1021 aggcctttcc caatattcat ccacctcaga taccagcaac tcccccatcg tatgaatctg
     1081 tagatgacat taatgctgat aagaatatct cttctgcaca gattgttggt cctggaccca
     1141 agccagaagc ctctgcaaag cttccttcca gacctgcaga taactatgac aactttgtcc
     1201 taccagagtt gccatctgtg ccagacacac taccaactgc atctgctggt gccagcacct
     1261 cagcatctga agacattgac tttgatgatc tttcccggag gtttgaagag ctgaaaaaga
     1321 aaacataggt ctcttaaacc aggcaacttt cacgttttgg gagttgagac tgagcaattt
     1381 ctccttgtaa caaagaatct ccatgaaatt ctgtttcatc tgttaaccgt cactcagcac
     1441 aacactccct ctgggctctc ttcctgctcc tccagattct gctgctttcc agttctctgt
     1501 tgatcctgag actaacaatt ggagactgag gccagagcaa ctggctcctg gcagctgtgc
     1561 ttgtccgttt cctgtcagag tgatcccagg tttcctcctg gcccgtccca tggtccctcc
     1621 acaggagtgt gagaggatgg gggaagcact gtgggaagac caccaaagat ggctggacag
     1681 tgggagagag cacgttgtga agcatcccag cctcgtgttg aggttccaga cttagaaaca
     1741 gacccctctg tacaggggga ttgtggtgag tgagaatcaa ggccaccttg tgtgttttct
     1801 cactctcgaa tgcaagtggg agagggaaaa tgactcggga cgccattgta acggttcctg
     1861 gaagctgggc cctctcattg gcatatacag tactcctcgc tgcagggcac tgtcccaccg
     1921 ggatccagtt gcaaagtttg tcttgacagt tgaaggcctc gcttagttgt actggattct
     1981 cagggagccc tctgtggcct tttgctttgc gtgctgtttc ccttgtacca gagggcggca
     2041 ccgtggaaat tctgttttcc ctgtagcata ttgtgttgga ttgcattact ggcagagaaa
     2101 ggacaaggtg ccattcaagt cctagggtgg gcttccagct gccttaatag aagtactcaa
     2161 gtcttttggg tagtgagctg gaaagcctac aggaaaagag gggtacctgt tttcatttga
     2221 aaactttgat tcatggaacc tttaaaacta atctcagaaa aatttttggt gcccatgcag
     2281 ctgtagttgt tcactgcttt cctggatgga tgggactctt atgtcataac ttctgttact
     2341 cctttggccc atagctaagg tcatccttcc ccacaggggt ggctttggga ttggatgata
     2401 cagcttttgc ttctgtgtag tatacctgta catacttgtt tcaggcagcc tttctttaat
     2461 gttttcagtt ggtttgtatt ctgtagctca gtagctgcta ataaagttaa agatcctgtg
     2521 tcctgctttc tctactgttt ggtcagatga tgaagtataa atctggattt tagtggagaa
     2581 tattttaacc aagtgatcca tgtaaaccag cccttagttt caggattcct ggagcagtac
     2641 agagctcatt ttgatctctg tatttagata gcccagatag catctgtgaa tttccttggt
     2701 cttcctcagt ggcagggctc cgcatctgcc atgcctcatc cttggatgtt tatttttagt
     2761 aagttgcagt gagctcactt ctgactgctt catttcccgc agccatcctg ggccatgggt
     2821 ggtgagttct gttacttgct gcttggcagc agagctagtt tgtctcgtag tattcttgca
     2881 ttgttaatcc tatcttgaca gtgccaagat ccataagaac ttgggaccaa ggggattttg
     2941 attcctaact tacagaatta aaaacaaagt atattataat tttaaagcta tgccatgcaa
     3001 agataagtag taatacgcta ataaatacta agccaagtag gagataacag gattaaggta
     3061 gttcatctaa aacttcagtg tcactgccca ctgctgtcgt ggctgcttgc tcagatgagg
     3121 ttttttgggg ccaactgatt cctaacaaat ttgagagctt ctgtagcagt gtatctatta
     3181 gtgcagcttc tttctgagta ttgaagacaa aaagagaaaa gtgagaaaat tgaaattact
     3241 gctaatagtg gagtaaaaaa agtaccaaag atttttaaaa aattagtaaa tgtaaagata
     3301 ctatttcttt aataatttgc agtcaggcat tggagtgttt ccactaatgg ttgcgagcca
     3361 actatttata ggacaatggc tatagaatat tatgtagtct tataaattgg gtttcctagg
     3421 aagatagctg gcagagcttt tgaaactaat aaagggaata tgatactgtg cattataaat
     3481 tatgtcagcc cgtcatctta ggggcagtta cagtggagct ttcccagtga tataacagca
     3541 tgctagttta tcttttagtt acctacctta agcgactttc tttcttttcc aaaggccatg
     3601 agaatggccg aaacgaaaag attaattacc acaagtacca tcaagatgac actggtgagg
     3661 atcagaaagg tgcccaggac tgggtctaaa gcgaaaacct gggaaaacaa taagaacact
     3721 tgcagtgact gacaatttag tttatacttt acaaacataa gctatatgct agtgtttggg
     3781 atcttatcag aagaaaagct tatccgaagg aaacttaggg agagagtcaa aagataataa
     3841 gaaggaaaat acttttaaat ggacagaaga gcaggaatga ctcattttaa ggaggttaag
     3901 ataattgtga ctgcagggct ttcacaagaa aacaggtgag ttgcagtgga attggaaatg
     3961 attcaaatgt cacatgagtt ttggcaaaaa atacatctaa tcatggaagc taaaagtggg
     4021 aggggggtaa gcaaacactg aaagattaaa tgcataccag aaatgagaat gcacactgtc
     4081 tctgccatat caagaaacta caaccctaaa cacaggagca ttccaataaa agttaacagg
     4141 ttggccacag aaaa
//
