LOCUS       NM_001270975            4561 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens IST1 factor associated with ESCRT-III (IST1),
            transcript variant 1, mRNA.
ACCESSION   NM_001270975
VERSION     NM_001270975.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Chen,H., Cai,Z., Dong,X., Chen,W., Cao,C., Zheng,S., Wu,J.,
            Zhong,D., Cheng,S., Gao,Y. and Shou,J.
  TITLE     Cigarette smoking-related OLC1 overexpression associated with poor
            prognosis in bladder urothelial carcinoma
  JOURNAL   Life Sci 351, 122821 (2024)
   PUBMED   38880167
  REMARK    GeneRIF: Cigarette smoking-related OLC1 overexpression associated
            with poor prognosis in bladder urothelial carcinoma.
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Cada,A.K., Pavlin,M.R., Castillo,J.P., Tong,A.B., Larsen,K.P.,
            Ren,X., Yokom,A.L., Tsai,F.C., Shiah,J.V., Bassereau,P.M.,
            Bustamante,C.J. and Hurley,J.H.
  TITLE     Friction-driven membrane scission by the human ESCRT-III proteins
            CHMP1B and IST1
  JOURNAL   Proc Natl Acad Sci U S A 119 (29), e2204536119 (2022)
   PUBMED   35858336
  REMARK    GeneRIF: Friction-driven membrane scission by the human ESCRT-III
            proteins CHMP1B and IST1.
REFERENCE   3  (bases 1 to 4561)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 4561)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4561)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   6  (bases 1 to 4561)
  AUTHORS   Agromayor,M., Carlton,J.G., Phelan,J.P., Matthews,D.R.,
            Carlin,L.M., Ameer-Beg,S., Bowers,K. and Martin-Serrano,J.
  TITLE     Essential role of hIST1 in cytokinesis
  JOURNAL   Mol Biol Cell 20 (5), 1374-1387 (2009)
   PUBMED   19129480
  REMARK    GeneRIF: hIST1 is essential for cytokinesis in mammalian cells.
REFERENCE   7  (bases 1 to 4561)
  AUTHORS   Bajorek,M., Morita,E., Skalicky,J.J., Morham,S.G., Babst,M. and
            Sundquist,W.I.
  TITLE     Biochemical analyses of human IST1 and its function in cytokinesis
  JOURNAL   Mol Biol Cell 20 (5), 1360-1373 (2009)
   PUBMED   19129479
  REMARK    GeneRIF: IST1 and CHMP1 act together to recruit and modulate
            specific VPS4 activities required during the final stages of cell
            division.
REFERENCE   8  (bases 1 to 4561)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   9  (bases 1 to 4561)
  AUTHORS   Yuan,J., Ma,J., Zheng,H., Shi,T., Sun,W., Zhang,Q., Lin,D.,
            Zhang,K., He,J., Mao,Y., Gao,X., Gao,P., Han,N., Fu,G., Xiao,T.,
            Gao,Y., Ma,D. and Cheng,S.
  TITLE     Overexpression of OLC1, cigarette smoke, and human lung
            tumorigenesis
  JOURNAL   J Natl Cancer Inst 100 (22), 1592-1605 (2008)
   PUBMED   19001599
  REMARK    GeneRIF: OLC1 (KIAA0174) is a candidate oncogene in lung cancer
            whose expression may be regulated by exposure to cigarette smoke.
REFERENCE   10 (bases 1 to 4561)
  AUTHORS   Kaye,F.J.
  TITLE     Defining a candidate lung cancer gene
  JOURNAL   J Natl Cancer Inst 100 (22), 1564-1565 (2008)
   PUBMED   19001597
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC103745.1 and AC009127.10.
            
            On May 17, 2019 this sequence version replaced NM_001270975.1.
            
            Summary: This gene encodes a protein with MIT-interacting motifs
            that interacts with components of endosomal sorting complexes
            required for transport (ESCRT). ESCRT functions in vesicle budding,
            such as that which occurs during membrane abscission in
            cytokinesis. There is a pseudogene for this gene on chromosome 19.
            Alternatively spliced transcript variants encoding multiple
            isoforms have been observed for this gene. [provided by RefSeq, Aug
            2012].
            
            Transcript Variant: This variant (1) encodes isoform a (also known
            as hIST1c).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.123498.1,
                                           SRR1660803.201406.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378799.11/ ENSP00000368076.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2365              BC103745.1         17-2381
            2366-4561           AC009127.10        81185-83380         c
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.2"
     gene            1..4561
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /note="IST1 factor associated with ESCRT-III"
                     /db_xref="GeneID:9798"
                     /db_xref="HGNC:HGNC:28977"
                     /db_xref="MIM:616434"
     exon            1..59
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            60..162
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..1175
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /note="isoform a is encoded by transcript variant 1; IST1
                     homolog; charged multivesicular body protein 8; putative
                     MAPK-activating protein PM28; overexpressed in lung cancer
                     1; increased sodium tolerance 1 homolog; IST1, ESCRT-III
                     associated factor; IST1, endosomal sorting complex
                     required for transport-III component"
                     /codon_start=1
                     /product="IST1 homolog isoform a"
                     /protein_id="NP_001257904.1"
                     /db_xref="CCDS:CCDS59272.1"
                     /db_xref="GeneID:9798"
                     /db_xref="HGNC:HGNC:28977"
                     /db_xref="MIM:616434"
                     /translation="MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYL
                     AAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVST
                     LIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKI
                     LVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFT
                     APVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIP
                     ATPPSYESVDDINADKNISSAQIVGPGPKPEASAKLPSRPADNYDNFVLPELPSVPDT
                     LPTASAGASTSASEDIDFDDLSRRFEELKKKT"
     exon            163..343
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            344..431
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            432..515
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            516..626
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            627..833
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            834..926
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            927..975
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            976..4561
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2347..2352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /note="hexamer: AATAAA"
     polyA_site      2367
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /note="major polyA site"
     regulatory      3972..3977
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /note="hexamer: AATAAA"
     polyA_site      4001
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
     regulatory      4504..4509
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
                     /note="hexamer: ATTACA"
     polyA_site      4544
                     /gene="IST1"
                     /gene_synonym="CHMP8; OLC1"
ORIGIN      
        1 attttggatg gtgaaccctg aagtcggtgt ctgctgcgtt cacggcagga ttcggttagg
       61 aggaacagca cagcatgctg ggctctggat ttaaagctga gcgcttaaga gtgaatttga
      121 gattagtcat aaatcgcctt aaactattgg agaaaaagaa aacggaactg gcccagaaag
      181 caaggaagga gattgctgac tatctggctg ctgggaaaga tgaacgagct cggatccgtg
      241 tggagcacat tatccgggaa gactacctcg tggaggccat ggagatcctg gagctgtact
      301 gtgacctgct gctggctcgg tttggcctta tccagtctat gaaggaacta gattctggtc
      361 tggctgaatc tgtgtctaca ttgatctggg ctgctcctcg actccagtca gaagtggctg
      421 agttgaaaat agttgctgat cagctctgtg ccaagtatag caaggaatat ggcaagctat
      481 gtaggaccaa ccagattgga actgtgaatg acaggctaat gcacaagctg agtgtggaag
      541 ccccacccaa aatcctggtg gagagatacc tgattgaaat tgcaaagaat tacaacgtac
      601 cctatgaacc tgactctgtg gtcatggcag aagctcctcc tggggtagag acagatctta
      661 ttgatgttgg attcacagat gatgtgaaga aaggaggccc tggaagagga gggagtggtg
      721 gcttcacagc accagttggt ggacctgatg gaacggtgcc aatgcccatg cccatgccca
      781 tgcctatgcc atctgcaaat acgcctttct catatccact gccaaaggga ccatcagatt
      841 tcaatggact gccaatgggg acttatcagg cctttcccaa tattcatcca cctcagatac
      901 cagcaactcc cccatcgtat gaatctgtag atgacattaa tgctgataag aatatctctt
      961 ctgcacagat tgttggtcct ggacccaagc cagaagcctc tgcaaagctt ccttccagac
     1021 ctgcagataa ctatgacaac tttgtcctac cagagttgcc atctgtgcca gacacactac
     1081 caactgcatc tgctggtgcc agcacctcag catctgaaga cattgacttt gatgatcttt
     1141 cccggaggtt tgaagagctg aaaaagaaaa cataggtctc ttaaaccagg caactttcac
     1201 gttttgggag ttgagactga gcaatttctc cttgtaacaa agaatctcca tgaaattctg
     1261 tttcatctgt taaccgtcac tcagcacaac actccctctg ggctctcttc ctgctcctcc
     1321 agattctgct gctttccagt tctctgttga tcctgagact aacaattgga gactgaggcc
     1381 agagcaactg gctcctggca gctgtgcttg tccgtttcct gtcagagtga tcccaggttt
     1441 cctcctggcc cgtcccatgg tccctccaca ggagtgtgag aggatggggg aagcactgtg
     1501 ggaagaccac caaagatggc tggacagtgg gagagagcac gttgtgaagc atcccagcct
     1561 cgtgttgagg ttccagactt agaaacagac ccctctgtac agggggattg tggtgagtga
     1621 gaatcaaggc caccttgtgt gttttctcac tctcgaatgc aagtgggaga gggaaaatga
     1681 ctcgggacgc cattgtaacg gttcctggaa gctgggccct ctcattggca tatacagtac
     1741 tcctcgctgc agggcactgt cccaccggga tccagttgca aagtttgtct tgacagttga
     1801 aggcctcgct tagttgtact ggattctcag ggagccctct gtggcctttt gctttgcgtg
     1861 ctgtttccct tgtaccagag ggcggcaccg tggaaattct gttttccctg tagcatattg
     1921 tgttggattg cattactggc agagaaagga caaggtgcca ttcaagtcct agggtgggct
     1981 tccagctgcc ttaatagaag tactcaagtc ttttgggtag tgagctggaa agcctacagg
     2041 aaaagagggg tacctgtttt catttgaaaa ctttgattca tggaaccttt aaaactaatc
     2101 tcagaaaaat ttttggtgcc catgcagctg tagttgttca ctgctttcct ggatggatgg
     2161 gactcttatg tcataacttc tgttactcct ttggcccata gctaaggtca tccttcccca
     2221 caggggtggc tttgggattg gatgatacag cttttgcttc tgtgtagtat acctgtacat
     2281 acttgtttca ggcagccttt ctttaatgtt ttcagttggt ttgtattctg tagctcagta
     2341 gctgctaata aagttaaaga tcctgtgtcc tgctttctct actgtttggt cagatgatga
     2401 agtataaatc tggattttag tggagaatat tttaaccaag tgatccatgt aaaccagccc
     2461 ttagtttcag gattcctgga gcagtacaga gctcattttg atctctgtat ttagatagcc
     2521 cagatagcat ctgtgaattt ccttggtctt cctcagtggc agggctccgc atctgccatg
     2581 cctcatcctt ggatgtttat ttttagtaag ttgcagtgag ctcacttctg actgcttcat
     2641 ttcccgcagc catcctgggc catgggtggt gagttctgtt acttgctgct tggcagcaga
     2701 gctagtttgt ctcgtagtat tcttgcattg ttaatcctat cttgacagtg ccaagatcca
     2761 taagaacttg ggaccaaggg gattttgatt cctaacttac agaattaaaa acaaagtata
     2821 ttataatttt aaagctatgc catgcaaaga taagtagtaa tacgctaata aatactaagc
     2881 caagtaggag ataacaggat taaggtagtt catctaaaac ttcagtgtca ctgcccactg
     2941 ctgtcgtggc tgcttgctca gatgaggttt tttggggcca actgattcct aacaaatttg
     3001 agagcttctg tagcagtgta tctattagtg cagcttcttt ctgagtattg aagacaaaaa
     3061 gagaaaagtg agaaaattga aattactgct aatagtggag taaaaaaagt accaaagatt
     3121 tttaaaaaat tagtaaatgt aaagatacta tttctttaat aatttgcagt caggcattgg
     3181 agtgtttcca ctaatggttg cgagccaact atttatagga caatggctat agaatattat
     3241 gtagtcttat aaattgggtt tcctaggaag atagctggca gagcttttga aactaataaa
     3301 gggaatatga tactgtgcat tataaattat gtcagcccgt catcttaggg gcagttacag
     3361 tggagctttc ccagtgatat aacagcatgc tagtttatct tttagttacc taccttaagc
     3421 gactttcttt cttttccaaa ggccatgaga atggccgaaa cgaaaagatt aattaccaca
     3481 agtaccatca agatgacact ggtgaggatc agaaaggtgc ccaggactgg gtctaaagcg
     3541 aaaacctggg aaaacaataa gaacacttgc agtgactgac aatttagttt atactttaca
     3601 aacataagct atatgctagt gtttgggatc ttatcagaag aaaagcttat ccgaaggaaa
     3661 cttagggaga gagtcaaaag ataataagaa ggaaaatact tttaaatgga cagaagagca
     3721 ggaatgactc attttaagga ggttaagata attgtgactg cagggctttc acaagaaaac
     3781 aggtgagttg cagtggaatt ggaaatgatt caaatgtcac atgagttttg gcaaaaaata
     3841 catctaatca tggaagctaa aagtgggagg ggggtaagca aacactgaaa gattaaatgc
     3901 ataccagaaa tgagaatgca cactgtctct gccatatcaa gaaactacaa ccctaaacac
     3961 aggagcattc caataaaagt taacaggttg gccacagaaa actggctttt tagtattttt
     4021 ttttttccat tgtcattttt aaaggtacaa gcattgagtt ctgccattct ttacttttct
     4081 gcatacataa atatcaagtt ctgggcttat tggaatggtt cccataacaa aaactgttgt
     4141 ttaaaattta gatgttcttt ttcaacaaat ggtatttatt ttcccttggt attggcccat
     4201 tcattgttgc cactagggca ctgtttaaaa atatcccaac agaggggttg attacagtca
     4261 atgccaccct gatggaaatc tatatggcaa aatattttca agcaccaaac tgttttacct
     4321 acttaaaaat gattattata acaatttcag gtcaaccaga taatgtgggc aatctctgag
     4381 tttgtgatac aagacttatt tccaataagt ttatttttat gtacaatttt actgtttatt
     4441 ttttgtttgc tctatttgtt ttttaatgtt ttttacttta tgtttactgt atttttatgt
     4501 acaattacat ccaaagcaga aaagttcctt ttttatcccc aaaaggaggt gtcagcaaaa
     4561 a
//
