LOCUS       NM_001379150            6563 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens insulin receptor substrate 4 (IRS4), transcript
            variant 2, mRNA.
ACCESSION   NM_001379150 XM_005262220
VERSION     NM_001379150.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Lauffer,P., Naafs,J.C., Bikker,H., Garrelfs,M.R., Mooij,C.F.,
            Boelen,A., Zwaveling-Soonawala,N. and van Trotsenburg,A.S.P.
  TITLE     Further Delineation of Central Congenital Hypothyroidism due to
            Variants in TBL1X and IRS4
  JOURNAL   Thyroid 33 (7), 880-884 (2023)
   PUBMED   36860195
  REMARK    GeneRIF: Further Delineation of Central Congenital Hypothyroidism
            due to Variants in TBL1X and IRS4.
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 6563)
  AUTHORS   Ortega,M.A., Fraile-Martinez,O., Garcia-Montero,C.,
            Borja-Vergel,S., Torres-Carranza,D., Pekarek,L., Arribas,C.B., De
            Leon-Luis,J.A., Sanchez-Rojo,C., Alvarez-Mon,M.A.,
            Garcia-Honduvilla,N., Bujan,J., Coca,S., Alvarez-Mon,M., Saez,M.A.
            and Guijarro,L.G.
  TITLE     Patients with Invasive Lobular Carcinoma Show a Significant
            Increase in IRS-4 Expression Compared to Infiltrative Ductal
            Carcinoma-A Histopathological Study
  JOURNAL   Medicina (Kaunas) 58 (6), 722 (2022)
   PUBMED   35743985
  REMARK    GeneRIF: Patients with Invasive Lobular Carcinoma Show a
            Significant Increase in IRS-4 Expression Compared to Infiltrative
            Ductal Carcinoma-A Histopathological Study.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6563)
  AUTHORS   Zhang,Y., Xiong,X., Zhu,Q., Zhang,J., Chen,S., Wang,Y., Cao,J.,
            Chen,L., Hou,L., Zhao,X., Hao,P., Chen,J., Zhuang,M., Li,D. and
            Fan,G.
  TITLE     FER-mediated phosphorylation and PIK3R2 recruitment on IRS4
            promotes AKT activation and tumorigenesis in ovarian cancer cells
  JOURNAL   Elife 11, e76183 (2022)
   PUBMED   35550247
  REMARK    GeneRIF: FER-mediated phosphorylation and PIK3R2 recruitment on
            IRS4 promotes AKT activation and tumorigenesis in ovarian cancer
            cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6563)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   6  (bases 1 to 6563)
  AUTHORS   Uchida,T., Myers,M.G. Jr. and White,M.F.
  TITLE     IRS-4 mediates protein kinase B signaling during insulin
            stimulation without promoting antiapoptosis
  JOURNAL   Mol Cell Biol 20 (1), 126-138 (2000)
   PUBMED   10594015
REFERENCE   7  (bases 1 to 6563)
  AUTHORS   Koval,A.P., Karas,M., Zick,Y. and LeRoith,D.
  TITLE     Interplay of the proto-oncogene proteins CrkL and CrkII in
            insulin-like growth factor-I receptor-mediated signal transduction
  JOURNAL   J Biol Chem 273 (24), 14780-14787 (1998)
   PUBMED   9614078
REFERENCE   8  (bases 1 to 6563)
  AUTHORS   White,M.F.
  TITLE     The IRS-signalling system: a network of docking proteins that
            mediate insulin action
  JOURNAL   Mol Cell Biochem 182 (1-2), 3-11 (1998)
   PUBMED   9609109
  REMARK    Review article
REFERENCE   9  (bases 1 to 6563)
  AUTHORS   Fantin,V.R., Sparling,J.D., Slot,J.W., Keller,S.R., Lienhard,G.E.
            and Lavan,B.E.
  TITLE     Characterization of insulin receptor substrate 4 in human embryonic
            kidney 293 cells
  JOURNAL   J Biol Chem 273 (17), 10726-10732 (1998)
   PUBMED   9553137
REFERENCE   10 (bases 1 to 6563)
  AUTHORS   Lavan,B.E., Fantin,V.R., Chang,E.T., Lane,W.S., Keller,S.R. and
            Lienhard,G.E.
  TITLE     A novel 160-kDa phosphotyrosine protein in insulin-treated
            embryonic kidney cells is a new member of the insulin receptor
            substrate family
  JOURNAL   J Biol Chem 272 (34), 21403-21407 (1997)
   PUBMED   9261155
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL035425.13.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Mar 24, 2020 this sequence version replaced XM_005262220.2.
            
            Summary: IRS4 encodes the insulin receptor substrate 4, a
            cytoplasmic protein that contains many potential tyrosine and
            serine/threonine phosphorylation sites. Tyrosine-phosphorylated
            IRS4 protein has been shown to associate with cytoplasmic
            signalling molecules that contain SH2 domains. The IRS4 protein is
            phosphorylated by the insulin receptor tyrosine kinase upon
            receptor stimulation.. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.2509762.1,
                                           SRR18074969.593920.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142586, SAMEA2144120
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372129.4/ ENSP00000361202.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3985              AL035425.13        117862-121846       c
            3986-6563           AL035425.13        105229-107806       c
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq22.3"
     gene            1..6563
                     /gene="IRS4"
                     /gene_synonym="CHNG9; IRS-4; PY160"
                     /note="insulin receptor substrate 4"
                     /db_xref="GeneID:8471"
                     /db_xref="HGNC:HGNC:6128"
                     /db_xref="MIM:300904"
     exon            1..3985
                     /gene="IRS4"
                     /gene_synonym="CHNG9; IRS-4; PY160"
                     /inference="alignment:Splign:2.1.0"
     CDS             220..3990
                     /gene="IRS4"
                     /gene_synonym="CHNG9; IRS-4; PY160"
                     /note="isoform 2 is encoded by transcript variant 2;
                     pp160; phosphoprotein of 160 kDa; 160 kDa phosphotyrosine
                     protein"
                     /codon_start=1
                     /product="insulin receptor substrate 4 isoform 2"
                     /protein_id="NP_001366079.1"
                     /db_xref="CCDS:CCDS94648.1"
                     /db_xref="GeneID:8471"
                     /db_xref="HGNC:HGNC:6128"
                     /db_xref="MIM:300904"
                     /translation="MASCSFTRDQATRRLRGAAAAAAAALAAVVTTPLLSSGTPTALI
                     GTGSSCPGAMWLSTATGSRSDSESEEEDLPVGEEVCKRGYLRKQKHGHRRYFVLKLET
                     ADAPARLEYYENARKFRHSVRAAAAAAAAAASGAAIPPLIPPRRVITLYQCFSVSQRA
                     DARYRHLIALFTQDEYFAMVAENESEQESWYLLLSRLILESKRRRCGTLGAQPDGEPA
                     ALAAAAAAEPPFYKDVWQVIVKPRGLGHRKELSGVFRLCLTDEEVVFVRLNTEVASVV
                     VQLLSIRRCGHSEQYFFLEVGRSTVIGPGELWMQVDDCVVAQNMHELFLEKMRALCAD
                     EYRARCRSYSISIGAHLLTLLSARRHLGLVPLEPGGWLRRSRFEQFCHLRAIGDGEDE
                     MLFTRRFVTPSEPVAHSRRGRLHLPRGRRSRRAVSVPASFFRRLAPSPARPRHPAEAP
                     NNGARLSSEVSGSGSGNFGEEGNPQGKEDQEGSGGDYMPMNNWGSGNGRGSGGGQGSN
                     GQGSSSHSSGGNQCSGEGQGSRGGQGSNGQGSGGNQCSRDGQGTAGGHGSGGGQRPGG
                     GHGSGGGQGPGDGHGSGGGKNSGGGKGSGSGKGSDGDGERGKSLKKRSYFGKLTQSKQ
                     QQMPPPPPPPPPPPPAGGTGGKGKSGGRFRLYFCVDRGATKECKEAKEVKDAEIPEGA
                     ARGPHRARAFDEDEDDPYVPMRPGVATPLVSSSDYMPMAPQNVSASKKRHSRSPFEDS
                     RGYMMMFPRVSPPPAPSPPKAPDTNKEDDSKDNDSESDYMFMAPGAGAIPKNPRNPQG
                     GSSSKSWSSYFSLPNPFRSSPLGQNDNSEYVPMLPGKFLGRGLDKEVSYNWDPKDAAS
                     KPSGEGSFSKPGDGGSPSKPSDHEPPKNKAKRPNRLSFITKGYKIKPKPQKPTHEQRE
                     ADSSSDYVNMDFTKRESNTPAPSTQGLPDSWGIIAEPRQSAFSNYVNVEFGVPFPNPA
                     NDLSDLLRAIPRANPLSLDSARWPLPPLPLSATGSNAIEEEGDYIEVIFNSAMTPAMA
                     LADSAIRYDAETGRIYVVDPFSECCMDISLSPSRCSEPPPVARLLQEEEQERRRPQSR
                     SQSFFAAARAAVSAFPTDSLERDLSPSSAPAVASAAEPTLALSQVVAAASALAAAPGI
                     GAAAAAAGFDSASARWFQPVANAADAEAVRGAQDVAGGSNPGAHNPSANLARGDNQAG
                     GAAAAAAAPEPPPRSRRVPRPPEREDSDNDDDTHVRMDFARRDNQFDSPKRE"
     exon            3986..6563
                     /gene="IRS4"
                     /gene_synonym="CHNG9; IRS-4; PY160"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4225
                     /gene="IRS4"
                     /gene_synonym="CHNG9; IRS-4; PY160"
                     /note="major polyA site"
     regulatory      6541..6546
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IRS4"
                     /gene_synonym="CHNG9; IRS-4; PY160"
                     /note="hexamer: AATAAA"
     polyA_site      6563
                     /gene="IRS4"
                     /gene_synonym="CHNG9; IRS-4; PY160"
                     /note="major polyA site"
ORIGIN      
        1 attgcgccgg tagcaggatc cgcagcggcc gccgcgtgag gctgtggtca cgtgttgttt
       61 tgcccgctgt cgtgcgctca gagtggagtg ggcgggggcg cgggccaagg caggaggggg
      121 ctggggcggg cagacgaggg tagacagcct cttcccccaa ccctcccttt cctgaattcc
      181 ccctcctcct cgctcacctt aaaaccatcg tgcatcacca tggcgagttg ctccttcact
      241 cgcgaccaag cgacaagaag actaagaggt gcagcagcgg cggcagcggc agctctagca
      301 gcagtggtga ccaccccgct tctttcctcg ggaaccccga ccgcactcat tgggaccggg
      361 tcgtcttgtc cgggagccat gtggctctcc acggccactg gctcccggtc agactccgag
      421 tccgaagagg aggacctgcc cgtcggggag gaagtctgca aacgcggcta cctgcggaaa
      481 cagaagcatg ggcacaggcg ctacttcgtg ctcaaactcg agactgctga cgccccagct
      541 cggctggaat actacgaaaa tgccaggaag ttccggcaca gtgtccgcgc cgcggcggct
      601 gcagcagcgg cggccgcctc tggcgccgcg atccccccgc tcattccacc gcggcgcgtg
      661 atcaccctat accagtgctt ttccgtgagc cagcgagcag atgcaaggta ccgacacctc
      721 attgctcttt tcacccaaga cgaatacttc gcgatggtgg ccgagaacga gtcggagcag
      781 gaaagctggt acttgctgct cagccgcctc atcctcgaga gcaagcgccg ccgctgcggc
      841 acgctcggcg cgcagccgga cggagagccg gccgcgctgg cggcggcagc ggcggcggag
      901 ccacccttct ataaagatgt gtggcaggta atagtcaaac ccagggggct ggggcacaga
      961 aaagagctga gcggcgtgtt ccggctgtgt ctaaccgacg aggaggtcgt gtttgtgagg
     1021 ctgaacaccg aagtggccag cgtggtcgtc cagctcctga gcatccgtcg ctgtggacac
     1081 tcggagcagt atttcttctt ggaagtaggc aggtccactg tcatcggtcc gggagagctc
     1141 tggatgcagg tcgatgactg tgtggttgcc caaaacatgc atgagctgtt tttggagaag
     1201 atgagagcct tgtgtgcaga cgaatacaga gcccgctgcc gcagctacag catcagcatc
     1261 ggcgcccacc tgttaaccct gctgtccgct aggaggcacc tgggcttggt gccgctcgag
     1321 ccgggaggct ggctcagaag gtcccgcttt gagcagtttt gccacctcag ggccatcggc
     1381 gacggggaag acgagatgct tttcaccagg cgcttcgtaa cacccagcga gcctgtggcc
     1441 cactccaggc gaggaagact gcacctgccc agagggcgca ggtcaaggag agcggtttca
     1501 gtgccggcca gcttttttcg ccgcttagca cccagcccag cacgtccccg gcaccctgca
     1561 gaagccccga acaatggagc tcgcctgtct tctgaagtgt ctggttctgg ctctggcaac
     1621 tttggggagg aaggcaatcc ccagggcaaa gaagatcagg aaggaagcgg aggtgactac
     1681 atgcctatga acaattgggg ctcaggaaat ggccggggct caggaggtgg ccagggctca
     1741 aatggccaag gctccagtag ccatagctcg ggaggaaacc agtgttcagg cgagggacag
     1801 ggatcccgag gtggtcaggg ctcaaatggc cagggctcag gaggaaacca gtgctctaga
     1861 gatggccagg gcaccgcagg tgggcacggt tcaggtggtg gccagagacc tggaggtggg
     1921 catggctcag gtggtggcca gggacctgga gatggccatg gctcaggtgg tggcaagaac
     1981 tctggggggg gcaaaggctc aggaagtggg aaaggatccg atggtgatgg tgaacgtgga
     2041 aaatctctga agaaaagatc ctattttggc aaattaactc aaagcaagca acagcaaatg
     2101 ccaccacctc caccacctcc tcctccaccc ccaccagctg gaggaactgg tggaaaaggg
     2161 aagtctgggg gaagattcag actttatttt tgtgttgaca gaggagccac gaaagaatgc
     2221 aaagaagcca aagaagtgaa agatgcagag atcccagaag gtgcagctcg aggtccccac
     2281 agagccagag cttttgatga agatgaggat gacccatacg tgccaatgag gccaggggtg
     2341 gccacccctc ttgtaagctc cagtgattat atgccaatgg ctcctcaaaa tgtctctgct
     2401 tcaaaaaagc gccactctcg atcccctttt gaagattcaa gagggtacat gatgatgttt
     2461 cccagagtga gcccaccacc tgctccgagt cctccaaaag cacctgatac taataaagag
     2521 gatgactcaa aggacaatga cagtgagagt gactacatgt ttatggctcc tggagccggt
     2581 gcaattccaa aaaaccccag aaatcctcag ggtggctctt cctccaaaag ttggagctcc
     2641 tacttctctc taccaaaccc ttttcggagc tcacctttgg gacagaatga caacagtgag
     2701 tatgtgccaa tgttacctgg aaagttcctg gggaggggcc tagacaaaga agtctcctat
     2761 aactgggacc ccaaagatgc agcttcaaag ccttcaggtg agggatcatt ctcaaagcct
     2821 ggagatgggg gatcaccttc aaagccttca gatcatgagc ccccaaagaa taaagctaag
     2881 agacctaacc gactttcttt tattacaaaa ggatataaaa tcaagccaaa accacaaaag
     2941 cccacacatg agcagagaga agctgacagc tctagtgact acgtcaacat ggacttcact
     3001 aaaagagaga gcaatacacc agctccctct actcaaggac taccagattc gtggggcata
     3061 attgctgaac ccagacagtc agccttttct aattatgtga atgttgagtt tggagtgcca
     3121 tttccaaatc cagcaaacga cctctcagat cttttaagag ctataccacg tgccaacccc
     3181 ttatctctgg acagtgctag gtggccactt cctccccttc ccctcagtgc tacaggtagc
     3241 aatgctattg aggaagaggg tgactacatt gaagtaattt tcaactcagc aatgacacca
     3301 gccatggctc ttgctgacag tgccattcgc tatgatgctg aaacaggtcg aatctatgtg
     3361 gtcgacccat tttctgagtg ctgtatggat atttctctct cccccagccg atgttctgaa
     3421 ccaccacctg tagctaggct gctgcaggaa gaagagcagg agagaagacg cccacaaagc
     3481 cgttctcaaa gtttctttgc agcagccaga gccgctgtct ctgcttttcc aacagacagc
     3541 ctcgagagag acctttcccc atcctcagcc ccggctgtcg cttcggctgc agagccgact
     3601 ttagccctca gccaagttgt agctgcggcc tccgcgctcg ccgcagcccc gggcatcggc
     3661 gcagcagccg cagctgctgg atttgactcc gcctctgccc gctggtttca acctgttgct
     3721 aatgctgctg atgccgaagc agtaagggga gcccaagacg ttgccggtgg ctcgaaccct
     3781 ggagcccaca acccatctgc aaaccttgcc agaggtgata accaggctgg cggggctgcc
     3841 gctgcagctg ccgctccgga accaccacct cgcagtcgcc gggtgccaag acccccggag
     3901 agagaagatt ctgacaacga cgacgacact cacgtgagaa tggattttgc cagacgtgat
     3961 aatcagttcg actctcccaa aagagagtga ttatattgcc acaaaaacac attttagaag
     4021 aaaagtgatg cttatggaat tttatcttcg tctactccag atctgcctaa aggaaagaaa
     4081 actcagaggc ttctgagtag aaagcaaccc aaatttctac aagcctacaa gtggactctg
     4141 gcaattgaat gatcctattc atgcagatca ttcatactgc tgcttatgga tccagtgatt
     4201 tctaattaac aatatttaca ctgtgctttg ttttttgttt tctggttttc ctccccagtc
     4261 tgtccctttg aaaggaaaca gatactatac aagtgagcat ctcataactc tttccctctt
     4321 taaaaaaaag taacttagaa tacatataaa tttggtcaat gccaatttta ataatgtaaa
     4381 atatttagat tcaattttga agttgaaaaa ctttaaacca gggcacataa agaaaaaaca
     4441 gctgtcataa ttggaaggaa ggtatgatct ggaatctttt ttccgagtat tacacacaca
     4501 aaacatttct tgatcaatga attaaatgtc aactgaactg atatcttgat ggggcattac
     4561 cagtatttta aaggcaggac taaagggcag attatttcaa gttttgctca ttagcaaaac
     4621 tgcaacattc tgctcatgtt atatgtcaat gaaactaata tcttgattga gcatttccaa
     4681 cattttaaag gcagaactga gaaacagcta tttgttcaca tacaacactg tgatgttctg
     4741 cctgaaaatc gattgagtat ttccaaacct attatttttc atttattgtg aaacctacat
     4801 ttacctccta aaagaaacaa agctcttcaa aagtctcttc ccaaggcccc tccctgcttc
     4861 tctgggcctc tccccacttt caacctctat tctacttttt cttattcttt ctccattttc
     4921 cccaatgcac actcttcctc ttatgcccat ggctactata aagagatcaa tttcccatat
     4981 tcaagaaatt agtgtttact tatcaatcaa tattctgaca aaaacttaag ccaaaaagaa
     5041 cctcttataa ggccttgttt ccttctggat attgagccaa tataagatgt accaaattaa
     5101 aaaaaaaaat cagagcaagc tcccccccgt tctatttttg cttgcctctg atgtaaactg
     5161 aattaagatt ttaggtacct ggacctttga gaacgtgaca ctagcaaggt ggtgaccaca
     5221 gagaataggt acaaaggtct tgaagcttgc ttagagcttt gttccgtggt ggcacacaaa
     5281 tcttggcttg ctgtaccatc attggcaaac ctcatcatat cacagttggg tgtgtttgta
     5341 tgtgtgtgag cactagtgtg tgtgtgtaca tattcgtgtg tatgcacact ttgtgtacct
     5401 gtgtacatgt gattgtagat gtacctgttg tgtgtaaact ctgttaaaat gaaatttgct
     5461 tcgcatttaa tgtgcaactt tgaagtagtg taatatttat ctaaaggttt tcactacttg
     5521 gttttaatgt ctttgaacgt gtacttctat cttcaagcaa gcttatagta cgtgttgcta
     5581 aactaacttt tccaaagaac cgagttgact tctatttttc cttgcctacc aaccaaaggg
     5641 atggtgggct cttttttccc tcttgtaaat tgtgctgagg agaggacgtg gctatgagat
     5701 cagtcattgt tccagttact ggtcactgct agggcatcaa ctttatcagt tccttcagac
     5761 tcttctcttt ttccaaccta atcaaataac gcagtgtggc ttttgttcat tataacaacg
     5821 cgctctttag gcaacttttt ttcaagcctt ctgtcaccat tttatttttt caagtaagtt
     5881 aagtaataat tgttcagaat tgtgggaaaa aagattactt gggggaaaat actgtatcac
     5941 tttatttgca tattaggttc agaagctgcc agcagacagg gtacagaggg tacagcacag
     6001 atgtaacagt tgctgaataa atgaatgaga aacctcatac acagtagtac gtattataca
     6061 aatagtaaca ggtacacata gtcacaggta ctgcataaca aatgcaatct tgttctcatt
     6121 atttattagt ttacagacaa ttaatttatt cagtattgtg ctggaagaat ctttgtggaa
     6181 tatgtttacc aacttatcac ttaccttcca gctatcagtt catgctgcta agaaaaaaca
     6241 atgatggaat atttttgtgt gtgtgacctc tacatttaat ttgatatcag gcatagtcac
     6301 ttggtcgtta aaaactgaaa ctttacagtt ccaccagcta attacatctg ctatttctgc
     6361 ttcacagata tgctttgagt tgctttggct ttgtcttcct ttgtaaatac tcttgtactt
     6421 tcttttcctt gtaaacatct cgtttgcagt tttgggagac gactttgcaa ctctgtatag
     6481 tgtttatata tatatgtata tctatatata tctgacttgc ctttccatac agctactcac
     6541 aataaatgtg ttcatgtaaa tta
//
