LOCUS       NM_003749               8156 bp    mRNA    linear   PRI 04-DEC-2024
DEFINITION  Homo sapiens insulin receptor substrate 2 (IRS2), mRNA.
ACCESSION   NM_003749
VERSION     NM_003749.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Nouri,S., Mahmoudi,T., Hojjati,F., Najafabadi,Z.N., Shafiee,R.,
            Sayedsalehi,S., Dehghanitafti,A., Ardalani,A., Kohansal,K.,
            Rezamand,G., Asadi,A., Nobakht,H., Dabiri,R., Farahani,H.,
            Tabaeian,S.P. and Zali,M.R.
  TITLE     Insulin receptor substrate 2 gene Gly1057Asp polymorphism is a risk
            factor for nonalcoholic fatty liver disease
  JOURNAL   Lab Med 55 (2), 215-219 (2024)
   PUBMED   37481466
  REMARK    GeneRIF: Insulin receptor substrate 2 gene Gly1057Asp polymorphism
            is a risk factor for nonalcoholic fatty liver disease.
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Fajardo,C.M., Cerda,A., Bortolin,R.H., de Oliveira,R.,
            Stefani,T.I.M., Dos Santos,M.A., Braga,A.A., Dorea,E.L.,
            Bernik,M.M.S., Bastos,G.M., Sampaio,M.F., Damasceno,N.R.T.,
            Verlengia,R., de Oliveira,M.R.M., Hirata,M.H. and Hirata,R.D.C.
  TITLE     Influence of polymorphisms in IRS1, IRS2, MC3R, and MC4R on
            metabolic and inflammatory status and food intake in Brazilian
            adults: An exploratory pilot study
  JOURNAL   Nutr Res 119, 21-32 (2023)
   PUBMED   37716291
  REMARK    GeneRIF: Influence of polymorphisms in IRS1, IRS2, MC3R, and MC4R
            on metabolic and inflammatory status and food intake in Brazilian
            adults: An exploratory pilot study.
REFERENCE   3  (bases 1 to 8156)
  AUTHORS   Furuta,H., Sheng,Y., Takahashi,A., Nagano,R., Kataoka,N.,
            Perks,C.M., Barker,R., Hakuno,F. and Takahashi,S.I.
  TITLE     The IGF-Independent Role of IRS-2 in the Secretion of MMP-9
            Enhances the Growth of Prostate Carcinoma Cell Line PC3
  JOURNAL   Int J Mol Sci 24 (20), 15065 (2023)
   PUBMED   37894751
  REMARK    GeneRIF: The IGF-Independent Role of IRS-2 in the Secretion of
            MMP-9 Enhances the Growth of Prostate Carcinoma Cell Line PC3.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8156)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   5  (bases 1 to 8156)
  AUTHORS   Luo,Q., Ling,Z., Huang,X. and Zuo,Y.
  TITLE     Association of IRS-1 and IRS-2 polymorphisms with predisposition to
            type-2 diabetes (T2D): a meta-analysis and trial sequential
            analysis
  JOURNAL   Nucleosides Nucleotides Nucleic Acids 42 (11), 837-851 (2023)
   PUBMED   37173295
  REMARK    GeneRIF: Association of IRS-1 and IRS-2 polymorphisms with
            predisposition to type-2 diabetes (T2D): a meta-analysis and trial
            sequential analysis.
REFERENCE   6  (bases 1 to 8156)
  AUTHORS   Platanias,L.C., Uddin,S., Yetter,A., Sun,X.J. and White,M.F.
  TITLE     The type I interferon receptor mediates tyrosine phosphorylation of
            insulin receptor substrate 2
  JOURNAL   J Biol Chem 271 (1), 278-282 (1996)
   PUBMED   8550573
REFERENCE   7  (bases 1 to 8156)
  AUTHORS   Johnston,J.A., Wang,L.M., Hanson,E.P., Sun,X.J., White,M.F.,
            Oakes,S.A., Pierce,J.H. and O'Shea,J.J.
  TITLE     Interleukins 2, 4, 7, and 15 stimulate tyrosine phosphorylation of
            insulin receptor substrates 1 and 2 in T cells. Potential role of
            JAK kinases
  JOURNAL   J Biol Chem 270 (48), 28527-28530 (1995)
   PUBMED   7499365
REFERENCE   8  (bases 1 to 8156)
  AUTHORS   Patti,M.E., Sun,X.J., Bruening,J.C., Araki,E., Lipes,M.A.,
            White,M.F. and Kahn,C.R.
  TITLE     4PS/insulin receptor substrate (IRS)-2 is the alternative substrate
            of the insulin receptor in IRS-1-deficient mice
  JOURNAL   J Biol Chem 270 (42), 24670-24673 (1995)
   PUBMED   7559579
REFERENCE   9  (bases 1 to 8156)
  AUTHORS   Sun,X.J., Wang,L.M., Zhang,Y., Yenush,L., Myers,M.G. Jr.,
            Glasheen,E., Lane,W.S., Pierce,J.H. and White,M.F.
  TITLE     Role of IRS-2 in insulin and cytokine signalling
  JOURNAL   Nature 377 (6545), 173-177 (1995)
   PUBMED   7675087
REFERENCE   10 (bases 1 to 8156)
  AUTHORS   Ridderstrale,M., Degerman,E. and Tornqvist,H.
  TITLE     Growth hormone stimulates the tyrosine phosphorylation of the
            insulin receptor substrate-1 and its association with
            phosphatidylinositol 3-kinase in primary adipocytes
  JOURNAL   J Biol Chem 270 (8), 3471-3474 (1995)
   PUBMED   7876077
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL162497.20, AF073310.1,
            AB050954.1, AF385933.1, CA952668.1, BC026044.1, AF161416.1 and
            CA416129.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003749.2.
            
            Summary: This gene encodes the insulin receptor substrate 2, a
            cytoplasmic signaling molecule that mediates effects of insulin,
            insulin-like growth factor 1, and other cytokines by acting as a
            molecular adaptor between diverse receptor tyrosine kinases and
            downstream effectors. The product of this gene is phosphorylated by
            the insulin receptor tyrosine kinase upon receptor stimulation, as
            well as by an interleukin 4 receptor-associated kinase in response
            to IL4 treatment. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF073310.1, SRR1660803.92050.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000375856.5/ ENSP00000365016.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AL162497.20        126402-126417       c
            17-97               AF073310.1         2-82
            98-747              AB050954.1         1967-2616
            748-1638            AF073310.1         737-1627
            1639-2697           AF385933.1         1250-2308
            2698-3142           AF073310.1         2687-3131
            3143-4534           AF385933.1         2754-4145
            4535-4542           AF073310.1         4521-4528
            4543-4930           CA952668.1         30-417
            4931-5606           BC026044.1         431-1106
            5607-5617           AF073310.1         5587-5597
            5618-5636           AF073310.1         5599-5617
            5637-5661           AF073310.1         5619-5643
            5662-5732           AF161416.1         81-151
            5733-6328           AF073310.1         5714-6309
            6329-6429           AF161416.1         748-848
            6430-6695           AF161416.1         850-1115
            6696-7009           AF161416.1         1117-1430
            7010-7014           CA416129.1         17-21               c
            7015-8156           AL162497.20        92529-93670         c
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q34"
     gene            1..8156
                     /gene="IRS2"
                     /gene_synonym="IRS-2"
                     /note="insulin receptor substrate 2"
                     /db_xref="GeneID:8660"
                     /db_xref="HGNC:HGNC:6126"
                     /db_xref="MIM:600797"
     exon            1..4542
                     /gene="IRS2"
                     /gene_synonym="IRS-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             531..4547
                     /gene="IRS2"
                     /gene_synonym="IRS-2"
                     /codon_start=1
                     /product="insulin receptor substrate 2"
                     /protein_id="NP_003740.2"
                     /db_xref="CCDS:CCDS9510.1"
                     /db_xref="GeneID:8660"
                     /db_xref="HGNC:HGNC:6126"
                     /db_xref="MIM:600797"
                     /translation="MASPPRHGPPGPASGDGPNLNNNNNNNNHSVRKCGYLRKQKHGH
                     KRFFVLRGPGAGGDEATAGGGSAPQPPRLEYYESEKKWRSKAGAPKRVIALDCCLNIN
                     KRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLVSEGRAAAGDAPPAAAPA
                     ASCSASLPGALGGSAGAAGAEDSYGLVAPATAAYREVWQVNLKPKGLGQSKNLTGVYR
                     LCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADD
                     SVVAQNIHETILEAMKALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPS
                     QTGLVRRSRTDSLAATPPAAKCSSCRVRTASEGDGGAAAGAAAAGARPVSVAGSPLSP
                     GPVRAPLSRSHTLSGGCGGRGSKVALLPAGGALQHSRSMSMPVAHSPPAATSPGSLSS
                     SSGHGSGSYPPPPGPHPPLPHPLHHGPGQRPSSGSASASGSPSDPGFMSLDEYGSSPG
                     DLRAFCSHRSNTPESIAETPPARDGGGGGEFYGYMTMDRPLSHCGRSYRRVSGDAAQD
                     LDRGLRKRTYSLTTPARQRPVPQPSSASLDEYTLMRATFSGSAGRLCPSCPASSPKVA
                     YHPYPEDYGDIEIGSHRSSSSNLGADDGYMPMTPGAALAGSGSGSCRSDDYMPMSPAS
                     VSAPKQILQPRAAAAAAAAVPSAGPAGPAPTSAAGRTFPASGGGYKASSPAESSPEDS
                     GYMRMWCGSKLSMEHADGKLLPNGDYLNVSPSDAVTTGTPPDFFSAALHPGGEPLRGV
                     PGCCYSSLPRSYKAPYTCGGDSDQYVLMSSPVGRILEEERLEPQATPGPSQAASAFGA
                     GPTQPPHPVVPSPVRPSGGRPEGFLGQRGRAVRPTRLSLEGLPSLPSMHEYPLPPEPK
                     SPGEYINIDFGEPGARLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTSSDSRQR
                     SPLSDYMNLDFSSPKSPKPGAPSGHPVGSLDGLLSPEASSPYPPLPPRPSASPSSSLQ
                     PPPPPPAPGELYRLPPASAVATAQGPGAASSLSSDTGDNGDYTEMAFGVAATPPQPIA
                     APPKPEAARVASPTSGVKRLSLMEQVSGVEAFLQASQPPDPHRGAKVIRADPQGGRRR
                     HSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSSEGGVGVGPGGGDEPPTSP
                     RQLQPAPPLAPQGRPWTPGQPGGLVGCPGSGGSPMRRETSAGFQNGLNYIAIDVREEP
                     GLPPQPQPPPPPLPQPGDKSSWGRTRSLGGLISAVGVGSTGGGCGGPGPGALPPANTY
                     ASIDFLSHHLKEATIVKE"
     exon            4543..8156
                     /gene="IRS2"
                     /gene_synonym="IRS-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6988..6993
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IRS2"
                     /gene_synonym="IRS-2"
                     /note="hexamer: AATAAA"
     polyA_site      7014
                     /gene="IRS2"
                     /gene_synonym="IRS-2"
                     /note="major polyA site"
     regulatory      8134..8139
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IRS2"
                     /gene_synonym="IRS-2"
                     /note="hexamer: AATAAA"
     polyA_site      8156
                     /gene="IRS2"
                     /gene_synonym="IRS-2"
ORIGIN      
        1 gttactcact gtgcggcggg gaccgcgacg agcccgggtc gccgttggca gcagcagcag
       61 caacaccagc agcagcagca gccccggcgg cggcgcggac cccgagcgcc cgggcgcacc
      121 ccggcttccc ggagcgcgac gcggcggcag cagccccggt gcggccgcgc gcgccttagg
      181 ctcggccccg cggctcgggg accccgactc ccggcccagc gagcgcgtcc cccggcgccg
      241 cccgagagcc cgaggaggca gcggccgcag gcagccgggg aggggggcgg ccaccgcccg
      301 cgccgggcat cctcaggagc cccagagcgc ggagggcgcg gcgccgccga gcggtgctgg
      361 cccccgcggg cctccccgga ccttccccac cgcctgggcc cgagggacgc gtgatcgggc
      421 gggcggccgg gcgcaagggt gggagggagc cgcccccgcc cgcgccccct ccgcccctcg
      481 ccccaacccc tgggcgccgg gcccgggccg cgcggcctga agcgcccgcg atggcgagcc
      541 cgccgcggca cgggccgccc gggccggcga gcggagacgg ccccaacctc aacaacaaca
      601 acaacaacaa caaccacagc gtgcgcaagt gcggctacct gcgcaagcag aagcatggcc
      661 acaagcgctt cttcgtgctg cgcggacccg gcgcgggcgg cgacgaggcg acggcgggcg
      721 gggggtcggc gccgcaaccg ccgcggctcg agtactacga gagcgagaaa aagtggcgga
      781 gcaaggcagg cgcgccgaaa cgggtgatcg ctctcgactg ctgcctgaac atcaacaagc
      841 gcgccgacgc caagcacaag tacctgatcg ccctctacac caaggacgag tacttcgccg
      901 tggccgccga gaacgagcag gagcaggagg gctggtaccg cgcgctcacc gacctggtca
      961 gcgagggccg cgcggccgcc ggagacgcgc cccccgccgc cgcgcccgcc gcgtcctgca
     1021 gcgcctccct gcccggcgcc ctgggcggct ctgccggcgc cgccggggcc gaggacagct
     1081 acgggctggt ggctcccgcc acggccgcct accgtgaggt gtggcaggtg aacctgaagc
     1141 ccaagggtct gggccagagc aagaacctga cgggggtgta ccgtctgtgc ctgtctgcgc
     1201 gcaccatcgg cttcgtgaag ctcaactgcg agcagccgtc ggtgacgctg cagctcatga
     1261 acatccgccg ctgcggccac tcggacagct tcttcttcat cgaggtgggc cgctcggccg
     1321 tcacaggccc cggcgagctg tggatgcagg cggacgactc ggtggtggcg cagaacatcc
     1381 acgagaccat cctggaggcc atgaaggcgc tcaaggagct cttcgagttc cggccgcgca
     1441 gtaagagcca atcgtcgggg tcgtcggcca cgcaccccat cagcgtcccc ggcgcgcgcc
     1501 gccaccacca cctggtcaac ctgcccccca gccagacggg cctggtgcgc cgctcgcgca
     1561 ccgacagcct ggccgccacc ccgccggcgg ccaagtgcag ctcgtgccgg gtgcgcaccg
     1621 ccagcgaggg cgacggcggc gcggcggcgg gagcggcggc cgcgggcgcc aggccggtgt
     1681 cggtggctgg gagccccctg agccccgggc cggtgcgcgc gcccctgagc cgctcgcaca
     1741 ccctgagcgg cggctgcggc ggccgcggga gcaaggtggc gctgctgccg gcagggggcg
     1801 cgctgcaaca cagccgctcc atgtccatgc ccgtggcgca ctcgccgccc gccgccacca
     1861 gccccggctc cctgtcgtcc agcagcggcc acggctcggg ctcctacccg ccgccgcccg
     1921 gcccgcaccc gcctctgccg catccgctgc accacggccc cggccagcgg ccctccagcg
     1981 gcagcgcctc cgcctcgggc tcccccagcg accccggctt catgtccctg gacgagtacg
     2041 gctccagccc aggcgacctg cgcgccttct gcagccaccg aagcaacacg cccgagtcca
     2101 tcgcggagac gcccccggcc cgagacggcg gcggcggcgg tgagttctac gggtacatga
     2161 ccatggacag gcccctgagc cactgtggcc gctcctaccg ccgggtctcg ggggacgcgg
     2221 cccaggacct ggaccgaggg ctgcgcaaga ggacctactc cctgaccacg ccagcccggc
     2281 agcggccggt gccccagccc tcctctgcct cgctggatga atacaccctg atgcgggcca
     2341 ccttctcggg cagcgcgggc cgcctctgcc cgtcctgccc cgcgtcctct cccaaggtgg
     2401 cctaccaccc ctacccagag gactacggag acatcgagat cggctcccac aggagctcca
     2461 gcagcaacct gggggcagac gacggctaca tgcccatgac gcccggcgcg gccctcgcgg
     2521 gcagtgggag cggcagctgc aggagcgacg actacatgcc catgagcccc gccagcgtgt
     2581 ccgcccccaa gcagatcttg cagcccaggg ccgccgccgc cgccgccgcc gccgtgcctt
     2641 ctgcggggcc tgcggggcca gcacccacct ctgcggcggg caggacattc ccggcgagcg
     2701 ggggcggcta caaggccagc tcgcccgccg agagctcccc cgaggacagt gggtacatgc
     2761 gcatgtggtg cggttccaag ctgtccatgg agcatgcaga tggcaagctg ctgcccaacg
     2821 gggactacct caacgtgtcc cccagcgacg cggtcaccac gggcaccccg cccgacttct
     2881 tctccgcagc cctgcacccc ggcggggagc cgctcagggg cgttcccggc tgctgctaca
     2941 gctccttgcc ccgctcctac aaggccccct acacctgtgg cggggacagc gaccagtacg
     3001 tgctcatgag ctcccccgtg gggcgcatcc tggaggagga gcgtctggag cctcaggcca
     3061 cgccagggcc cagccaggcg gccagcgcct tcggggccgg ccccacgcag ccccctcacc
     3121 ctgtagtgcc ttcgcccgtg cggcctagcg gcggccgccc ggagggcttc ttgggccagc
     3181 gcggccgggc ggtgaggccc acgcgcctgt ccctggaggg gctgcccagc ctgcccagca
     3241 tgcacgagta cccactgcca ccggagccca agagccccgg cgagtacatc aacatcgact
     3301 ttggcgagcc cggggcccgc ctgtcgccgc ccgcgcctcc cctgctggcg tcggcggcct
     3361 cgtcctcctc gctcttgtcc gccagcagcc cggcctcgtc gctgggctca ggcaccccgg
     3421 gcaccagcag cgacagccgg cagcggtctc cgctctccga ctacatgaac ctcgacttca
     3481 gctcccccaa gtctcctaag ccgggcgccc cgagcggcca ccccgtgggc tccttggacg
     3541 gcctcctgtc ccccgaggcc tcctccccgt atccgccgtt gcccccgcgt ccgtccgcgt
     3601 ccccgtcgtc gtctctgcag ccgccgccac cgccgccggc cccgggggag ctgtaccgcc
     3661 tgccccccgc ctcggccgtt gccaccgccc agggcccggg cgccgcctca tcgttgtcct
     3721 cggacaccgg ggacaatggt gactacaccg agatggcttt tggtgtggcc gccaccccgc
     3781 cgcaacctat cgcggccccc ccgaagccag aagctgcccg cgtggccagc ccgacgtcgg
     3841 gcgtgaagag gctgagcctc atggagcagg tgtcgggagt cgaggccttc ctgcaggcca
     3901 gccagccccc ggacccccac cgcggcgcca aggtcatccg cgcagacccg caggggggcc
     3961 gccgccgcca cagttccgag accttctcct ccaccacgac ggtcaccccc gtgtccccgt
     4021 ccttcgccca caaccccaag cgccacaact cggcctccgt ggaaaatgtc tctctcagga
     4081 aaagcagcga gggcggcgtg ggtgtcggcc ctggaggggg cgacgagccg cccacctccc
     4141 cacgacagtt gcagccggcg ccccctttgg caccgcaggg ccggccgtgg accccgggtc
     4201 agcccggggg cttggtcggt tgtcctggga gcggtggatc gcccatgcgc agagagacct
     4261 ctgccggctt ccagaatggt ctcaactaca tcgccatcga cgtgagggag gagcccgggc
     4321 tgccacccca gccgcagccg ccgccgccgc cgcttcctca gccgggagac aagagctcct
     4381 ggggccggac ccgaagcctc gggggtctca tcagcgctgt gggcgtcggc agcaccggcg
     4441 gcgggtgcgg ggggccgggt cccggtgccc tgccccctgc caacacctac gccagcattg
     4501 acttcttgtc ccaccacttg aaggaggcca ccatcgtgaa agagtgaaga tctgtctggc
     4561 tttatcacca ggatgtcaca tgtcagagag tatcattaaa agaagacgct cagcactgtt
     4621 tcagcccgaa gctgcttgca gttttctttt ggatctgagc aatgactgtg tttggaaaca
     4681 tctgtggact ctgttagatg aggcaccaac aaggcaaggt cacctgcctc tttcccttgt
     4741 tcccggatgg ggcattcatc attgtgctgt ttgcgttttg ttttgttttg ttttaacaaa
     4801 attagctgaa gaagttattc tcaagaaaat tggatgtttt cattggcctt cttaaattgt
     4861 ggccagtgtc ttttaatttc ttcttctttt ccttttggca aagcagatat aaccctcagc
     4921 atgctaggag agtgcacccg tacctatgga agtggtaaaa tctggtattt actggcttac
     4981 actcaaaacg accacagtcc tacctcagtt caaggtaaag ccggatttcc gtggcggggg
     5041 tcccacagga cctcctgtag tagcccctgc gctgtgtgtc tggagcgcgg tcctcggcct
     5101 tattgaaatg gtccaagtag acagctgctt gttggattcc agtgcaggta cctgcgatgt
     5161 ttacgtccac accgagccca gtgtgggact gacatttctc aatggaagtg aaatttggga
     5221 ttggactttg aagacggatt actaaataat aattattata tgtaactgaa gcaacctact
     5281 tttgaaaatc aactgtattg ggtagtggga ggtgggaggg aagggctttg ggaaggggat
     5341 gaatatctct ttttaccttt aacagacttg tttaatcttc tcgatgtaga tgtttatgta
     5401 ggtacttcac attgcaaacg ccttttattc tatttacaag ctcagatgtc tctgctctcc
     5461 tgaatcttgg gcatgccttt ctgtaaccaa aaatccctgt aggcgtgcta gcaattccag
     5521 ggtggtccgg gtttggcaga tttgattttt aaaaaacgta ttatctttaa taaaatgtta
     5581 ttatgtcaac cagtgaggct gccctgaaca aaaaaaacaa aaagaaaaaa aaaaaaggaa
     5641 agaaagaaac tgataaaaag aggcattcca gcccctatgt tattgatgga aaaagaaaaa
     5701 gaagaaaagc aatctcgcag tacatgttac ttgtcgaaaa aattccggac aagactaccc
     5761 ttgttttatg ttttcagtat tctgaaaata ccagtgtgtg gcagttctcg cagatgttac
     5821 ctaaaactgc tgaacttgac cggcagaatg ttctgccgtt ttctgctccc tcgacacttg
     5881 attggagggc tgtcgacctc tcctcccgtg ggggcttccc cagtgcctat cttctctgat
     5941 agtcatggag aggttacact aattcattgg agatgtaagt tgttggtttt gttttgtttt
     6001 gtttttagaa aaatatatat aaatatataa tagatatcta tcgctataga ataatgcatt
     6061 aataaaatga ggctttttta gaggaagacc aaaaaattca atgtcttaaa aatatattta
     6121 atggcaatgc aaaagtcttc ctgcttccgt gctgaacttt agaacagagg attgtattgc
     6181 aagacaaagt tgaatgtaaa gtgatctccc tgaacatttt taaggtttta cttttctgaa
     6241 attatacatc acagcagtgc ataggccata taatgttagc tggaaggtca atttcagtgt
     6301 atgatatact ttattaagat gtataaaaat cctgaagttt ttatttagtt ttgggaatag
     6361 gcatcaatgg gtggtatttg ctttgtaact ccccccaggt acgataggga ctgaatatgg
     6421 accctgctga aagcagtgta ttgacgcata tttaactcgc cctctatccg tagagtagtc
     6481 atgacactat acagatggtt cgtgttcata ctgcagctta aaacaagcaa aatacacaga
     6541 tgataatatg ctaaattttc ctctatcctg tacatttcac aaaaaggcat atgcaatatt
     6601 tacattttta atttagttta cagaatggaa ccaaaatgta taaatgttat gtttgctaaa
     6661 acttcacaat gtatattggg tctttgtaca ttttgcctga cttaccttaa atttaaaata
     6721 ttttttgcta tataaacttt aacagttatt aaacagtgtt ttctttttgg gtacgtattg
     6781 tttctggata tcaagatgtt aaatatattt cttgctattg tgatatgaca agagacttaa
     6841 cttatcttgc tctgtcttcc actgtacacg ctgtatatag gggtcaatgt gatgctgctg
     6901 gagacgagaa taaactggac tagaatagtg cattgtattt agtctgtatt gatcatggat
     6961 gccctcctta atagccatat gcaataaaat aaagtacatt atttatgaaa tgaatacagt
     7021 cctgaagatt ttttctgtac gaattctctg tttaacataa agccaatcta cttttagagt
     7081 ttgggagtaa gtgaggaaat actaggctcc taaaaatact tggatacccc ttttaatttt
     7141 aaaactttta ttctggaagc caaaaaatac ctgccagata acaacattta tgaaaagtaa
     7201 atgtgttata taacaaaaca gaacattcga tgtactctac aaaacagagt ccttatgacg
     7261 tgctggatgc cattctaaat acataacaaa tattaacttc ttgatagctc atgacaatct
     7321 taggaggtaa gaattgtcat cctcattcta cagaggagga gcccgaggta caaaaatgct
     7381 aagagacttg tgtgagagca cacagtccat gagtggtaaa accaggaccg ctccccagct
     7441 ctgcagtcca gaatctgctt atgcacctct gcttagtgct cccttcctga tgtgcacaca
     7501 gaagatacac caacgtgtac atcaagaatg ctccctccat gacatgcaca cagaagatgt
     7561 accaatgcat acattaagaa tgcttcctcc ggccgggcgt ggtggctcac acctgtaatc
     7621 ccagcacttt gggaggctga ggcaggcaga tcacctgagg tcaggagttc gagacaagcc
     7681 tgaccaacat ggagaaaccc cgtctctact aaaaatacaa aattagccag gcgtggtggc
     7741 gcatgcctgt aatcccagct actcgggagg ctgaggcagg agaatggctt gaacccggga
     7801 ggcagaggtt gccgtgagcc aagataatgc cattgcactc cagcctgggc aacaagagcg
     7861 aaactccgtc tcaaaaaaaa aaaaaaaaaa gaagaagaat gctgcctcca tgacacaaac
     7921 acagaagaag acataccgat gcatacatca agaatacaaa gaagaaaatc atcagatcca
     7981 ggaaaatgca attcaggaag cagaaaatag aaaattgatg ctgtgaattt ttatccagga
     8041 gagttttacc tccttgcttg aaacatttgt ccgtgcatcc actttgccct aaaatgatag
     8101 tcatgtaata atgtgtgtgt tatcttttga acaaataaaa tcagtgcaat ttggaa
//
