LOCUS       NM_005544               9771 bp    mRNA    linear   PRI 27-FEB-2025
DEFINITION  Homo sapiens insulin receptor substrate 1 (IRS1), mRNA.
ACCESSION   NM_005544
VERSION     NM_005544.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Asadi,A., Rostami,M., Shafiee,R., Ardalani,A., Dehghanitafti,A.,
            Golshadi,Z., Kohansal,K., Ghasemi,F., Najafi,M., Mahmoudi,T.,
            Rezamand,G., Dabiri,R., Nobakht,H., Farahani,H. and Tabaeian,S.P.
  TITLE     Association of IRS1 gene Pro512Ala polymorphism with nonalcoholic
            fatty liver disease
  JOURNAL   Arch Endocrinol Metab 68, e230216 (2024)
   PUBMED   39420901
  REMARK    GeneRIF: Association of IRS1 gene Pro512Ala polymorphism with
            nonalcoholic fatty liver disease.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  AUTHORS   Arner,P., Viguerie,N., Massier,L., Ryden,M., Astrup,A., Blaak,E.,
            Langin,D. and Andersson,D.P.
  TITLE     Sex differences in adipose insulin resistance are linked to
            obesity, lipolysis and insulin receptor substrate 1
  JOURNAL   Int J Obes (Lond) 48 (7), 934-940 (2024)
   PUBMED   38491191
  REMARK    GeneRIF: Sex differences in adipose insulin resistance are linked
            to obesity, lipolysis and insulin receptor substrate 1.
REFERENCE   3  (bases 1 to 9771)
  AUTHORS   Yu,F., Huang,D., Kuang,Y., Dong,J., Han,Q., Zhou,J. and Teng,X.
  TITLE     IRS1 promotes thyroid cancer metastasis through EMT and PI3K/AKT
            pathways
  JOURNAL   Clin Endocrinol (Oxf) 100 (3), 284-293 (2024)
   PUBMED   38172081
  REMARK    GeneRIF: IRS1 promotes thyroid cancer metastasis through EMT and
            PI3K/AKT pathways.
REFERENCE   4  (bases 1 to 9771)
  AUTHORS   Qu,X., Li,X.Y., Feng,Y., Wang,X., Li,L., Wang,Y.P. and Chu,Y.L.
  TITLE     sh- Ambra1 inhibits IRS-1/PI3K/Akt signalling pathway to reduce
            autophagy in gestational diabetes
  JOURNAL   Endokrynol Pol 75 (1), 61-70 (2024)
   PUBMED   38497391
  REMARK    GeneRIF: sh- Ambra1 inhibits IRS-1/PI3K/Akt signalling pathway to
            reduce autophagy in gestational diabetes.
REFERENCE   5  (bases 1 to 9771)
  AUTHORS   Zhou,H.L., Grimmett,Z.W., Venetos,N.M., Stomberski,C.T., Qian,Z.,
            McLaughlin,P.J., Bansal,P.K., Zhang,R., Reynolds,J.D., Premont,R.T.
            and Stamler,J.S.
  TITLE     An enzyme that selectively S-nitrosylates proteins to regulate
            insulin signaling
  JOURNAL   Cell 186 (26), 5812-5825 (2023)
   PUBMED   38056462
REFERENCE   6  (bases 1 to 9771)
  AUTHORS   Kharitonenkov,A., Schnekenburger,J., Chen,Z., Knyazev,P., Ali,S.,
            Zwick,E., White,M. and Ullrich,A.
  TITLE     Adapter function of protein-tyrosine phosphatase 1D in insulin
            receptor/insulin receptor substrate-1 interaction
  JOURNAL   J Biol Chem 270 (49), 29189-29193 (1995)
   PUBMED   7493946
REFERENCE   7  (bases 1 to 9771)
  AUTHORS   Wang,L., Hayashi,H., Mitani,Y., Ishii,K., Ohnishi,T., Niwa,Y.,
            Kido,H. and Ebina,Y.
  TITLE     Cloning of a cDNA encoding a 190-kDa insulin receptor
            substrate-1-like protein of simian COS cells
  JOURNAL   Biochem Biophys Res Commun 216 (1), 321-328 (1995)
   PUBMED   7488107
REFERENCE   8  (bases 1 to 9771)
  AUTHORS   Hadari,Y.R., Tzahar,E., Nadiv,O., Rothenberg,P., Roberts,C.T. Jr.,
            LeRoith,D., Yarden,Y. and Zick,Y.
  TITLE     Insulin and insulinomimetic agents induce activation of
            phosphatidylinositol 3'-kinase upon its association with pp185
            (IRS-1) in intact rat livers
  JOURNAL   J Biol Chem 267 (25), 17483-17486 (1992)
   PUBMED   1381348
  REMARK    Erratum:[J Biol Chem 1993 Apr 25;268(12):9156]
REFERENCE   9  (bases 1 to 9771)
  AUTHORS   Nishiyama,M. and Wands,J.R.
  TITLE     Cloning and increased expression of an insulin receptor
            substrate-1-like gene in human hepatocellular carcinoma
  JOURNAL   Biochem Biophys Res Commun 183 (1), 280-285 (1992)
   PUBMED   1311924
REFERENCE   10 (bases 1 to 9771)
  AUTHORS   Sun,X.J., Rothenberg,P., Kahn,C.R., Backer,J.M., Araki,E.,
            Wilden,P.A., Cahill,D.A., Goldstein,B.J. and White,M.F.
  TITLE     Structure of the insulin receptor substrate IRS-1 defines a unique
            signal transduction protein
  JOURNAL   Nature 352 (6330), 73-77 (1991)
   PUBMED   1648180
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010735.11 and BC053895.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 8, 2020 this sequence version replaced NM_005544.2.
            
            Summary: This gene encodes a protein which is phosphorylated by
            insulin receptor tyrosine kinase. Mutations in this gene are
            associated with type II diabetes and susceptibility to insulin
            resistance. [provided by RefSeq, Nov 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: S62539.1, BC053895.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2159912 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000305123.6/ ENSP00000304895.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1030              AC010735.11        78372-79401         c
            1031-6035           BC053895.1         1-5005
            6036-9771           AC010735.11        10893-14628         c
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q36.3"
     gene            1..9771
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
                     /note="insulin receptor substrate 1"
                     /db_xref="GeneID:3667"
                     /db_xref="HGNC:HGNC:6125"
                     /db_xref="MIM:147545"
     exon            1..4832
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             1083..4811
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
                     /codon_start=1
                     /product="insulin receptor substrate 1"
                     /protein_id="NP_005535.1"
                     /db_xref="CCDS:CCDS2463.1"
                     /db_xref="GeneID:3667"
                     /db_xref="HGNC:HGNC:6125"
                     /db_xref="MIM:147545"
                     /translation="MASPPESDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARL
                     EYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAE
                     QDSWYQALLQLHNRAKGHHDGAAALGAGGGGGSCSGSSGLGEAGEDLSYGDVPPGPAF
                     KEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSE
                     NFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRSKSQSSSNC
                     SNPISVPLRRHHLNNPPPSQVGLTRRSRTESITATSPASMVGGKPGSFRVRASSDGEG
                     TMSRPASVDGSPVSPSTNRTHAHRHRGSARLHPPLNHSRSIPMPASRCSPSATSPVSL
                     SSSSTSGHGSTSDCLFPRRSSASVSGSPSDGGFISSDEYGSSPCDFRSSFRSVTPDSL
                     GHTPPARGEEELSNYICMGGKGPSTLTAPNGHYILSRGGNGHRCTPGTGLGTSPALAG
                     DEAASAADLDNRFRKRTHSAGTSPTITHQKTPSQSSVASIEEYTEMMPAYPPGGGSGG
                     RLPGHRHSAFVPTRSYPEEGLEMHPLERRGGHHRPDSSTLHTDDGYMPMSPGVAPVPS
                     GRKGSGDYMPMSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGGCSPDIGGGPSSS
                     SSSSNAVPSGTSYGKLWTNGVGGHHSHVLPHPKPPVESSGGKLLPCTGDYMNMSPVGD
                     SNTSSPSDCYYGPEDPQHKPVLSYYSLPRSFKHTQRPGEPEEGARHQHLRLSTSSGRL
                     LYAATADDSSSSTSSDSLGGGYCGARLEPSLPHPHHQVLQPHLPRKVDTAAQTNSRLA
                     RPTRLSLGDPKASTLPRAREQQQQQQPLLHPPEPKSPGEYVNIEFGSDQSGYLSGPVA
                     FHSSPSVRCPSQLQPAPREEETGTEEYMKMDLGPGRRAAWQESTGVEMGRLGPAPPGA
                     ASICRPTRAVPSSRGDYMTMQMSCPRQSYVDTSPAAPVSYADMRTGIAAEEVSLPRAT
                     MAAASSSSAASASPTGPQGAAELAAHSSLLGGPQGPGGMSAFTRVNLSPNRNQSAKVI
                     RADPQGCRRRHSSETFSSTPSATRVGNTVPFGAGAAVGGGGGSSSSSEDVKRHSSASF
                     ENVWLRPGELGGAPKEPAKLCGAAGGLENGLNYIDLDLVKDFKQCPQECTPEPQPPPP
                     PPPHQPLGSGESSSTRRSSEDLSAYASISFQKQPEDRQ"
     exon            4833..9771
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5621..5626
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
                     /note="hexamer: ATTAAA"
     polyA_site      5639
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
                     /note="major polyA site"
     regulatory      5836..5841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
                     /note="hexamer: AATAAA"
     polyA_site      5861
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
                     /note="major polyA site"
     regulatory      9664..9669
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
                     /note="hexamer: AAGAAA"
     regulatory      9678..9683
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
                     /note="hexamer: AATGAA"
     polyA_site      9683
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
                     /note="major polyA site"
     polyA_site      9697
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
     regulatory      9748..9753
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
                     /note="hexamer: ATTAAA"
     polyA_site      9771
                     /gene="IRS1"
                     /gene_synonym="HIRS-1"
ORIGIN      
        1 ggcggctgcc cggctgccga gcgcggaggc tccgtcacgt gtttttctcc tccgagtgag
       61 acggcggcgc ggtcggaggg ggccggcgcg cagagccaga cgccgccgct tgttttggtt
      121 ggggctctcg gcaactctcc gaggaggagg aggaggaggg aggaggggag aagtaactgc
      181 agcggcagcg cctcccgagg aacaggcgtc ttccccgaac ccttcccaaa cctcccccat
      241 cccctctcgc ccttgtcccc tcccctcctc cccagccgcc tggagcgagg ggcagggatg
      301 agtctgtccc tccggccggt ccccagctgc agtggctgcc cggtatcgtt tcgcatggaa
      361 aagccacttt ctccacccgc cgagatgggc ccggatgggg gctgcagagg acgcgcccgc
      421 gggcggcggc agcagcagca gcagcagcag cagcaacagc aacagccgca gcgccgcggt
      481 ctctgcgact gagctggtat ttgggcggct ggtggcggct gggacggttg gggggtggga
      541 ggaggcgaag gaggagggag aaccccgtgc aacgttggga cttggcaacc cgcctccccc
      601 tgcccaagga tatttaattt gcctcgggaa tcgctgcttc cagaggggaa ctcaggaggg
      661 aaggcgcgcg cgcgcgcgcg ctcctggagg ggcaccgcag ggacccccga ctgtcgcctc
      721 cctgtgccgg actccagccg gggcgacgag agatgcatct tcgctccttc ctggtggcgg
      781 cggcggctga gaggagactt ggctctcgga ggatcggggc tgccctcacc ccggacgcac
      841 tgcctccccg ccgggcgtga agcgcccgaa aactccggtc gggctctctc ctgggctcag
      901 cagctgcgtc ctccttcagc tgcccctccc cggcgcgggg ggcggcgtgg atttcagagt
      961 cggggtttct gctgcctcca gccctgtttg catgtgccgg gccgcggcga ggagcctccg
     1021 ccccccaccc ggttgttttt cggagcctcc ctctgctcag cgttggtggt ggcggtggca
     1081 gcatggcgag ccctccggag agcgatggct tctcggacgt gcgcaaggtg ggctacctgc
     1141 gcaaacccaa gagcatgcac aaacgcttct tcgtactgcg cgcggccagc gaggctgggg
     1201 gcccggcgcg cctcgagtac tacgagaacg agaagaagtg gcggcacaag tcgagcgccc
     1261 ccaaacgctc gatccccctt gagagctgct tcaacatcaa caagcgggct gactccaaga
     1321 acaagcacct ggtggctctc tacacccggg acgagcactt tgccatcgcg gcggacagcg
     1381 aggccgagca agacagctgg taccaggctc tcctacagct gcacaaccgt gctaagggcc
     1441 accacgacgg agctgcggcc ctcggggcgg gaggtggtgg gggcagctgc agcggcagct
     1501 ccggccttgg tgaggctggg gaggacttga gctacggtga cgtgccccca ggacccgcat
     1561 tcaaagaggt ctggcaagtg atcctgaagc ccaagggcct gggtcagaca aagaacctga
     1621 ttggtatcta ccgcctttgc ctgaccagca agaccatcag cttcgtgaag ctgaactcgg
     1681 aggcagcggc cgtggtgctg cagctgatga acatcaggcg ctgtggccac tcggaaaact
     1741 tcttcttcat cgaggtgggc cgttctgccg tgacggggcc cggggagttc tggatgcagg
     1801 tggatgactc tgtggtggcc cagaacatgc acgagaccat cctggaggcc atgcgggcca
     1861 tgagtgatga gttccgccct cgcagcaaga gccagtcctc gtccaactgc tctaacccca
     1921 tcagcgtccc cctgcgccgg caccatctca acaatccccc gcccagccag gtggggctga
     1981 cccgccgatc acgcactgag agcatcaccg ccacctcccc ggccagcatg gtgggcggga
     2041 agccaggctc cttccgtgtc cgcgcctcca gtgacggcga aggcaccatg tcccgcccag
     2101 cctcggtgga cggcagccct gtgagtccca gcaccaacag aacccacgcc caccggcatc
     2161 ggggcagcgc ccggctgcac cccccgctca accacagccg ctccatcccc atgccggctt
     2221 cccgctgctc gccttcggcc accagcccgg tcagtctgtc gtccagtagc accagtggcc
     2281 atggctccac ctcggattgt ctcttcccac ggcgatctag tgcttcggtg tctggttccc
     2341 ccagcgatgg cggtttcatc tcctcggatg agtatggctc cagtccctgc gatttccgga
     2401 gttccttccg cagtgtcact ccggattccc tgggccacac cccaccagcc cgcggtgagg
     2461 aggagctaag caactatatc tgcatgggtg gcaaggggcc ctccaccctg accgccccca
     2521 acggtcacta cattttgtct cggggtggca atggccaccg ctgcacccca ggaacaggct
     2581 tgggcacgag tccagccttg gctggggatg aagcagccag tgctgcagat ctggataatc
     2641 ggttccgaaa gagaactcac tcggcaggca catcccctac cattacccac cagaagaccc
     2701 cgtcccagtc ctcagtggct tccattgagg agtacacaga gatgatgcct gcctacccac
     2761 caggaggtgg cagtggaggc cgactgccgg gacacaggca ctccgccttc gtgcccaccc
     2821 gctcctaccc agaggagggt ctggaaatgc accccttgga gcgtcggggg gggcaccacc
     2881 gcccagacag ctccaccctc cacacggatg atggctacat gcccatgtcc ccaggggtgg
     2941 ccccagtgcc cagtggccga aagggcagtg gagactatat gcccatgagc cccaagagcg
     3001 tatctgcccc acagcagatc atcaatccca tcagacgcca tccccagaga gtggacccca
     3061 atggctacat gatgatgtcc cccagcggtg gctgctctcc tgacattgga ggtggcccca
     3121 gcagcagcag cagcagcagc aacgccgtcc cttccgggac cagctatgga aagctgtgga
     3181 caaacggggt agggggccac cactctcatg tcttgcctca ccccaaaccc ccagtggaga
     3241 gcagcggtgg taagctctta ccttgcacag gtgactacat gaacatgtca ccagtggggg
     3301 actccaacac cagcagcccc tccgactgct actacggccc tgaggacccc cagcacaagc
     3361 cagtcctctc ctactactca ttgccaagat cctttaagca cacccagcgc cccggggagc
     3421 cggaggaggg tgcccggcat cagcacctcc gcctttccac tagctctggt cgccttctct
     3481 atgctgcaac agcagatgat tcttcctctt ccaccagcag cgacagcctg ggtgggggat
     3541 actgcggggc taggctggag cccagccttc cacatcccca ccatcaggtt ctgcagcccc
     3601 atctgcctcg aaaggtggac acagctgctc agaccaatag ccgcctggcc cggcccacga
     3661 ggctgtccct gggggatccc aaggccagca ccttacctcg ggcccgagag cagcagcagc
     3721 agcagcagcc cttgctgcac cctccagagc ccaagagccc gggggaatat gtcaatattg
     3781 aatttgggag tgatcagtct ggctacttgt ctggcccggt ggctttccac agctcacctt
     3841 ctgtcaggtg tccatcccag ctccagccag ctcccagaga ggaagagact ggcactgagg
     3901 agtacatgaa gatggacctg gggccgggcc ggagggcagc ctggcaggag agcactgggg
     3961 tcgagatggg cagactgggc cctgcacctc ccggggctgc tagcatttgc aggcctaccc
     4021 gggcagtgcc cagcagccgg ggtgactaca tgaccatgca gatgagttgt ccccgtcaga
     4081 gctacgtgga cacctcgcca gctgcccctg taagctatgc tgacatgcga acaggcattg
     4141 ctgcagagga ggtgagcctg cccagggcca ccatggctgc tgcctcctca tcctcagcag
     4201 cctctgcttc cccgactggg cctcaagggg cagcagagct ggctgcccac tcgtccctgc
     4261 tggggggccc acaaggacct gggggcatga gcgccttcac ccgggtgaac ctcagtccta
     4321 accgcaacca gagtgccaaa gtgatccgtg cagacccaca agggtgccgg cggaggcata
     4381 gctccgagac tttctcctca acacccagtg ccacccgggt gggcaacaca gtgccctttg
     4441 gagcgggggc agcagtaggg ggcggtggcg gtagcagcag cagcagcgag gatgtgaaac
     4501 gccacagctc tgcttccttt gagaatgtgt ggctgaggcc tggggagctt gggggagccc
     4561 ccaaggagcc agccaaactg tgtggggctg ctgggggttt ggagaatggt cttaactaca
     4621 tagacctgga tttggtcaag gacttcaaac agtgccctca ggagtgcacc cctgaaccgc
     4681 agcctccccc acccccaccc cctcatcaac ccctgggcag cggtgagagc agctccaccc
     4741 gccgctcaag tgaggattta agcgcctatg ccagcatcag tttccagaag cagccagagg
     4801 accgtcagta gctcaactgg acatcacagc agaatgaaga cctaaatgac ctcagcaaat
     4861 cctcttctaa ctcatgggta cccagactct aaatatttca tgattcacaa ctaggacctc
     4921 atatcttcct catcagtaga tggtacgatg catccatttc agtttgttta ctttatccaa
     4981 tcctcaggat ttcattgact gaactgcacg ttctatattg tgccaagcga aaaaaaaaaa
     5041 tgcactgtga caccagaata atgagtctgc ataaacttca tcttcaacct taaggactta
     5101 gctggccaca gtgagctgat gtgcccacca ccgtgtcatg agagaatggg tttactctca
     5161 atgcattttc aagatacatt tcatctgctg ctgaaactgt gtacgacaaa gcatcattgt
     5221 aaattatttc atacaaaact gttcacgttg ggtggagaga gtattaaata tttaacatag
     5281 gttttgattt atatgtgtaa ttttttaaat gaaaatgtaa cttttcttac agcacatctt
     5341 ttttttggat gtgggatgga ggtatacaat gttctgttgt aaagagtgga gcaaatgctt
     5401 aaaacaaggc ttaaaagagt agaatagggt atgatccttg ttttaagatt gtaattcaga
     5461 aaacataata taagaatcat agtgccatag atggttctca attgtatagt tatatttgct
     5521 gatactatct cttgtcatat aaacctgatg ttgagctgag ttccttataa gaattaatct
     5581 taattttgta ttttttcctg taagacaata ggccatgtta attaaactga agaaggatat
     5641 atttggctgg gtgttttcaa atgtcagctt aaaattggta attgaatgga agcaaaatta
     5701 taagaagagg aaattaaagt cttccattgc atgtattgta aacagaagga gatgggtgat
     5761 tccttcaatt caaaagctct ctttggaatg aacaatgtgg gcgtttgtaa attctggaaa
     5821 tgtctttcta ttcataataa actagatact gttgatcttt tcttctgtcc cctcccccca
     5881 ccacttctgt aagtttcctg ctctattccc accatttttt tctgtgcaca cattatgata
     5941 tatttcattt cctgcattgt cttgagaaag atggtaaggc aagtgagctg ttgctaacca
     6001 gaaattaaaa ttccagtaag tgtttttcat tatgaccagg gctatgtgtc accttcccta
     6061 agactcttac cttatctcat attttttgag aacttccagt gttacattat ttaactgaat
     6121 gtaattggcc catttgcctt ggtgggtgct ggcctattag tgattagtta acaaaacaca
     6181 gcgtacagag agcacagaaa agcttaatga cctgctactg aaacacctag ccagcagtga
     6241 aaatgttaat tcttttcttg tttggaaagt atacacgtct tggaattttt tccacgtgaa
     6301 aaacaaatgg caatgaatgc atttaaagat attgccgaca gatttttaaa tctttttacc
     6361 aggaaacttc ctaaaggtta aatgaattaa tgcaaatatc aggctccctc tgagtctgtg
     6421 ggagcctcta tctctctatc aggaattcgc atccctacta ttgggaggag caacatttta
     6481 tttctctgaa cgcctaagct ccctgggtgg gagtggggac tacaaggtag gggccagggt
     6541 tggagggcat tgtagtggct gctgcctcct gatgaactgt ttggggaccc cagctctact
     6601 caaaagggag cggagataaa tggaacccct cacactgctg aggcccgtgt tactgttcat
     6661 tcagccaggt ggcatgtacc tcacagactg ttgtgcagtg tccgttattg cagattttaa
     6721 tcatttgcat gctcatcaat ttctaagata aatagggtct agagtcataa gaatccattg
     6781 ttttcaagga acttgcagaa ttacatcatt tcctattagt agagagcact acccattttg
     6841 aaaatctgat atgaaagttg ttttttactc ttgtaaaaaa agactttctt agtcaaacta
     6901 acttttcata ttttcaagca ttctgattca tactcttgct agtggaagaa gagagcaagc
     6961 tgccctgctc ttttcctttg aggactgaaa tagttaaaga gaaatcaatg aacaaagtca
     7021 ctcccaacca ttttcctgta aagctggtta ttatttctca aggaacctac actttgaata
     7081 tgtgttaccg agatacctct acatgtggaa ttatcaacat gttttgaatg agagcagaaa
     7141 tgaacagact ggaaaaatct atctcttggt ttctatttct ctgacttttt gagtcgaaaa
     7201 gcataaaggt agaaattctt atttaagctg cttctagtgg tgcctgagct ggttttgatg
     7261 gtggcatcaa actaccgatt taaaactgga agttgctggt actcaaacca aaagttcata
     7321 ctctggcgac acgaagggtt tcctttgagc aacgtcagct gctgagtcct tgtgttcagt
     7381 tcccattgag gagagttggc ttttatccat ttcaaagcat ttgtaggcca gccaagggct
     7441 ttcattattg aggcttctag tggcctctgg ttaacctaga agttagtggg tttttcttga
     7501 tgacaccaac ctctcacagc gtttttcctt agagacttaa gcagagtttt aaaatcctct
     7561 tttgcgaaaa gaacaaatat gttttatgac tttgatgata tcttcattct gggcaaaaga
     7621 atggccctag aaccagctag aagtgaagag aatccattaa tgatcaacca cctgagtcaa
     7681 taatgagaaa tcagtactga tgttacattt gtggctattt cttgctgact ttcaaaggtc
     7741 aaggactctt gactaatcca gtgactgcaa aaatggatct actaaaagtc atctagccag
     7801 aagtagagat ttttaacctt tctttccctg gcttttgtct tctagctatc atttaaattt
     7861 gagacatttg aagtattaag aaacaatttt tctgtatggt aagaaacagt attttacaat
     7921 actgaagccc tgttttattc aatcttgcat cttgaataca atataccaca aagtcggaaa
     7981 ctttatattt atttactgca ggtggttaaa aaaagggggg aaagggtttc accatccact
     8041 gacaacgaga gccatgacaa atagtatcca tgtgcagtct tccaactgct ggtgacaatg
     8101 accccatatt tggtctcatg ctgcttttgc agagcactct gtaggttagt ccatcacaca
     8161 aggaggccct gaatccagac actgtgaatt aagaccttgg cggggagaga tgtgaccctt
     8221 ttggtaggaa tgggaagaag aatgggtgga agccaatatg aaatttcttc tttgcaatga
     8281 cttgacaggg gagttaatgt tcctaggatg ccatgaatga tgaatgttag ttggaggtaa
     8341 tgctgtatat gtgtgtgtgt gtgtgtgtgt gtgtttgtgt gtgtgtatat atatgtgtgt
     8401 gtgtatatat atgtgacatg tgtgtgtctt tgtgtgtgtg tatatatatg tgacgtgtgt
     8461 gtgggatgtg tgtgtgtata tatagatgaa tatatacaaa tatatagata tatacacaca
     8521 tatatagata tatatacaca tatatagata tatatacaca catatataga tacacatatg
     8581 tgtgtgtgtg tatatatata tatatatata tatagatgta taggcttgtg agaaacttga
     8641 gaggaagaag catgctcttc taggaatgtg aggaaatatg accttgccaa gactaaaaga
     8701 cctctagact gtgagctcag ttatggagaa caaaaacagc ttcatagtga gtagaacacc
     8761 gaggataaac actggggcca tgggtccttt ctgaggcagc gccacagaag atctttgtgg
     8821 tccttccgta gttctgtaag tctgtctcct aagtatgggt agagaatatg tagcctgttg
     8881 tgtgtctccc actacttgta aacagagcat cacattaggg gcagggagga ggtggaatga
     8941 tattggaggt gcttaaccct actcgaggaa ttaattatga ataaagagct tataattagc
     9001 taacatgact agaaaacaca tgacttaggt ggagagttag ctttcttttc tagtttgtgt
     9061 atgacttgcc atttgtgacg tatacaccaa aagatctggt gttttagact tctgccattc
     9121 acttggcatt taaatctctc tttgcttatg ctgttaacga gtatgccata ggataggaca
     9181 aattcagtaa acaggaaaac ttgtccatat ttgcatagac atttgtaggg tttttttttt
     9241 ctttttcttt ttagaacttc accattggct taagaatgta gttcccaaaa caattttttc
     9301 ttgcaaagta ctttccttac acctcttggc tacagggtgg gccaaattaa acatatatgt
     9361 attttcattt aatgtatgtg cagtttggtt tatcatctta agatggtggt gctgccccgg
     9421 tgctacttca tctgtgtaca caaagaccaa tgcatggtct gtattgctac caaaacattt
     9481 actgtatata tgtttataac atgtattatg tatatatgta atgggtgcca ggccaggtat
     9541 atatttttta tttagaagtg tttcactttt ccaagttttc tttatagtgt tatgcttatt
     9601 ttcaattttt tttttcctga ttctgtctgg tacttagaat tgtagtgtct tcatcatcaa
     9661 ttaaagaaaa ctgtctaaat gaattcatgg atgtaaatat tagtggtcct taatgtcttt
     9721 gattgctgga catgaaacaa actgccaatt aaattttgcg gagacatttg a
//
