LOCUS       NM_007199               8328 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens interleukin 1 receptor associated kinase 3 (IRAK3),
            transcript variant 1, mRNA.
ACCESSION   NM_007199
VERSION     NM_007199.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Watany,M.M., Elhosary,M.M., El-Horany,H.E. and El-Horany,M.E.
  TITLE     Methylation of Interleukin-1 receptor-associated kinase-3 and the
            risk of multiple sclerosis relapse/activity
  JOURNAL   Clin Immunol 266, 110327 (2024)
   PUBMED   39053866
  REMARK    GeneRIF: Methylation of Interleukin-1 receptor-associated kinase-3
            and the risk of multiple sclerosis relapse/activity.
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Liu,J., Copland,D.A., Clare,A.J., Gorski,M., Richards,B.T.,
            Scott,L., Theodoropoulou,S., Greferath,U., Cox,K., Shi,G.,
            Bell,O.H., Ou,K., Powell,J.L.B., Wu,J., Robles,L.M., Li,Y.,
            Nicholson,L.B., Coffey,P.J., Fletcher,E.L., Guymer,R., Radeke,M.J.,
            Heid,I.M., Hageman,G.S., Chan,Y.K. and Dick,A.D.
  TITLE     Replenishing IRAK-M expression in retinal pigment epithelium
            attenuates outer retinal degeneration
  JOURNAL   Sci Transl Med 16 (750), eadi4125 (2024)
   PUBMED   38838135
  REMARK    GeneRIF: Replenishing IRAK-M expression in retinal pigment
            epithelium attenuates outer retinal degeneration.
REFERENCE   3  (bases 1 to 8328)
  AUTHORS   Yang,K., Yang,L., Chen,X., Li,J., Zheng,B., Hu,J., Wang,H., Yu,Q.
            and Song,G.
  TITLE     Importance of serum IRAK3 as a biochemical marker in relation to
            severity and neurological outcome of human severe traumatic brain
            injury: A prospective longitudinal cohort study
  JOURNAL   Clin Chim Acta 553, 117754 (2024)
   PUBMED   38169195
  REMARK    GeneRIF: Importance of serum IRAK3 as a biochemical marker in
            relation to severity and neurological outcome of human severe
            traumatic brain injury: A prospective longitudinal cohort study.
REFERENCE   4  (bases 1 to 8328)
  AUTHORS   Turek,I., Nguyen,T.H., Galea,C., Abad,I., Freihat,L.,
            Manallack,D.T., Velkov,T. and Irving,H.
  TITLE     Mutations in the Vicinity of the IRAK3 Guanylate Cyclase Center
            Impact Its Subcellular Localization and Ability to Modulate
            Inflammatory Signaling in Immortalized Cell Lines
  JOURNAL   Int J Mol Sci 24 (10), 8572 (2023)
   PUBMED   37239919
  REMARK    GeneRIF: Mutations in the Vicinity of the IRAK3 Guanylate Cyclase
            Center Impact Its Subcellular Localization and Ability to Modulate
            Inflammatory Signaling in Immortalized Cell Lines.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8328)
  AUTHORS   Nakashima,K., Hirota,T., Obara,K., Shimizu,M., Jodo,A., Kameda,M.,
            Doi,S., Fujita,K., Shirakawa,T., Enomoto,T., Kishi,F.,
            Yoshihara,S., Matsumoto,K., Saito,H., Suzuki,Y., Nakamura,Y. and
            Tamari,M.
  TITLE     An association study of asthma and related phenotypes with
            polymorphisms in negative regulator molecules of the TLR signaling
            pathway
  JOURNAL   J Hum Genet 51 (4), 284-291 (2006)
   PUBMED   16432636
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 8328)
  AUTHORS   del Fresno,C., Otero,K., Gomez-Garcia,L., Gonzalez-Leon,M.C.,
            Soler-Ranger,L., Fuentes-Prior,P., Escoll,P., Baos,R., Caveda,L.,
            Garcia,F., Arnalich,F. and Lopez-Collazo,E.
  TITLE     Tumor cells deactivate human monocytes by up-regulating IL-1
            receptor associated kinase-M expression via CD44 and TLR4
  JOURNAL   J Immunol 174 (5), 3032-3040 (2005)
   PUBMED   15728517
  REMARK    GeneRIF: IRAK-M is rapidly upregulated in human monocytes
            pre-exposed to tumor cells and could be involved in deactivation of
            tumor-infiltrating monocytes mediated by tumor cells.
REFERENCE   7  (bases 1 to 8328)
  AUTHORS   Escoll,P., del Fresno,C., Garcia,L., Valles,G., Lendinez,M.J.,
            Arnalich,F. and Lopez-Collazo,E.
  TITLE     Rapid up-regulation of IRAK-M expression following a second
            endotoxin challenge in human monocytes and in monocytes isolated
            from septic patients
  JOURNAL   Biochem Biophys Res Commun 311 (2), 465-472 (2003)
   PUBMED   14592437
  REMARK    GeneRIF: Thus, our data indicate that IRAK-M could play a pivotal
            role in the process of endotoxin tolerance in human monocytes and
            provide evidence that PI3K is involved in regulating its
            expression.
REFERENCE   8  (bases 1 to 8328)
  AUTHORS   Bin,L.H., Xu,L.G. and Shu,H.B.
  TITLE     TIRP, a novel Toll/interleukin-1 receptor (TIR) domain-containing
            adapter protein involved in TIR signaling
  JOURNAL   J Biol Chem 278 (27), 24526-24532 (2003)
   PUBMED   12721283
REFERENCE   9  (bases 1 to 8328)
  AUTHORS   Kobayashi,K., Hernandez,L.D., Galan,J.E., Janeway,C.A. Jr.,
            Medzhitov,R. and Flavell,R.A.
  TITLE     IRAK-M is a negative regulator of Toll-like receptor signaling
  JOURNAL   Cell 110 (2), 191-202 (2002)
   PUBMED   12150927
REFERENCE   10 (bases 1 to 8328)
  AUTHORS   Wesche,H., Gao,X., Li,X., Kirschning,C.J., Stark,G.R. and Cao,Z.
  TITLE     IRAK-M is a novel member of the Pelle/interleukin-1
            receptor-associated kinase (IRAK) family
  JOURNAL   J Biol Chem 274 (27), 19403-19410 (1999)
   PUBMED   10383454
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA594800.1, BC057800.1,
            BP292474.1, AF113136.1, AC078889.20 and AL049435.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_007199.2.
            
            Summary: This gene encodes a member of the interleukin-1
            receptor-associated kinase protein family. Members of this family
            are essential components of the Toll/IL-R immune signal
            transduction pathways. This protein is primarily expressed in
            monocytes and macrophages and functions as a negative regulator of
            Toll-like receptor signaling. Mutations in this gene are associated
            with a susceptibility to asthma. Alternate splicing results in
            multiple transcript variants. [provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.25922.1,
                                           SRR1803613.87331.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261233.9/ ENSP00000261233.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 DA594800.1         337-345
            10-524              BC057800.1         1-515
            525-635             BP292474.1         472-582
            636-2036            BC057800.1         627-2027
            2037-2311           AF113136.1         2014-2288
            2312-6378           AC078889.20        118139-122205       c
            6379-8328           AL049435.1         746-2695
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q14.3"
     gene            1..8328
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /note="interleukin 1 receptor associated kinase 3"
                     /db_xref="GeneID:11213"
                     /db_xref="HGNC:HGNC:17020"
                     /db_xref="MIM:604459"
     exon            1..219
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..1877
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /EC_number="2.7.11.1"
                     /note="isoform a is encoded by transcript variant 1; IL-1
                     receptor-associated kinase M; inactive IL-1
                     receptor-associated kinase 3"
                     /codon_start=1
                     /product="interleukin-1 receptor-associated kinase 3
                     isoform a"
                     /protein_id="NP_009130.2"
                     /db_xref="CCDS:CCDS8975.1"
                     /db_xref="GeneID:11213"
                     /db_xref="HGNC:HGNC:17020"
                     /db_xref="MIM:604459"
                     /translation="MAGNCGARGALSAHTLLFDLPPALLGELCAVLDSCDGALGWRGL
                     AERLSSSWLDVRHIEKYVDQGKSGTRELLWSWAQKNKTIGDLLQVLQEMGHRRAIHLI
                     TNYGAVLSPSEKSYQEGGFPNILFKETANVTVDNVLIPEHNEKGILLKSSISFQNIIE
                     GTRNFHKDFLIGEGEIFEVYRVEIQNLTYAVKLFKQEKKMQCKKHWKRFLSELEVLLL
                     FHHPNILELAAYFTETEKFCLIYPYMRNGTLFDRLQCVGDTAPLPWHIRIGILIGISK
                     AIHYLHNVQPCSVICGSISSANILLDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSS
                     SKHLWYMPEEYIRQGKLSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRELME
                     KRGLDSCLSFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEVLNTLESTQASL
                     YFAEDPPTSLKSFRCPSPLFLENVPSIPVEDDESQNNNLLPSDEGLRIDRMTQKTPFE
                     CSQSEVMFLSLDKKPESKRNEEACNMPSSSCEESWFPKYIVPSQDLRPYKVNIDPSSE
                     APGHSCRSRPVESSCSSKFSWDEYEQYKKE"
     exon            220..402
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /inference="alignment:Splign:2.1.0"
     exon            403..467
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /inference="alignment:Splign:2.1.0"
     exon            468..522
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /inference="alignment:Splign:2.1.0"
     exon            523..674
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /inference="alignment:Splign:2.1.0"
     exon            675..739
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /inference="alignment:Splign:2.1.0"
     exon            740..854
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /inference="alignment:Splign:2.1.0"
     exon            855..973
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1172
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1235
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1400
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..8328
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2329..2334
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /note="hexamer: AATAGA"
     polyA_site      2349
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /note="major polyA site"
     regulatory      6954..6959
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /note="hexamer: AATAAA"
     polyA_site      6981
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
     regulatory      7102..7107
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /note="hexamer: AATAAA"
     polyA_site      7129
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
     regulatory      7903..7908
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
                     /note="hexamer: AATAAA"
     polyA_site      7932
                     /gene="IRAK3"
                     /gene_synonym="ASRT5; IRAKM"
ORIGIN      
        1 aggatttccg cggttgtgta acggcctgtc gcaggcgtgc agggacctgg actccgcctc
       61 gtccccgggg ctcgggcagc cgagccatgg cggggaactg tggggcccgc ggcgcgctgt
      121 cggcgcacac gctgctgttc gacctgccgc ccgcgctgct cggagagctc tgcgctgttc
      181 tggacagctg cgacggcgcg ctgggctggc gcggcctggc agagagactt tcaagcagct
      241 ggctggatgt tcgtcatatt gaaaagtatg tagaccaagg taaaagtgga acaagagaat
      301 tactttggtc ctgggcacag aaaaacaaga ccatcggtga ccttttacag gtcctccagg
      361 agatgggaca tcgtcgagct attcatttaa ttacaaacta tggagcagtg ttgagtcctt
      421 cagagaagag ttatcaggaa ggtggatttc caaatatatt attcaaggaa acagccaatg
      481 tcaccgtgga taatgttctt attcctgaac ataatgaaaa aggaatactg cttaaatctt
      541 ccatcagctt tcaaaatatc atagaaggaa ctagaaattt ccacaaagac ttcctaattg
      601 gagaaggaga gatttttgag gtatacagag tggagattca aaacctaaca tatgctgtca
      661 aattatttaa acaggagaaa aaaatgcagt gtaagaagca ttggaagagg tttttatctg
      721 agcttgaagt tttactactg tttcatcacc caaacatact agagttggct gcatatttta
      781 cagagactga gaagttctgt ctgatttatc catacatgag aaatggaaca ctttttgaca
      841 gattgcagtg tgtaggtgac acggccccac tcccttggca cattcgaatc ggtatattaa
      901 taggaatatc caaagccatt cactacctgc acaacgttca accatgctcg gtcatctgtg
      961 gcagtatatc aagtgcaaac atccttttgg atgatcagtt tcaacccaaa ctaactgatt
     1021 ttgccatggc acacttccgg tcccacctag aacatcagag ttgtaccata aatatgacca
     1081 gcagcagcag taaacatctg tggtacatgc cagaagagta catcagacag gggaaacttt
     1141 ccattaaaac agatgtctac agctttggaa ttgtaataat ggaagttcta acaggatgta
     1201 gagtagtgtt agatgatcca aaacatatcc agctgcggga tctccttaga gaattgatgg
     1261 agaagagagg cctggattca tgtctctcat ttctagataa gaaagtgcct ccctgccctc
     1321 ggaatttctc tgccaagctc ttctgtttgg caggccggtg tgctgcaacg cgggcaaagt
     1381 taagaccatc aatggatgaa gttttaaata ctcttgaaag tactcaagcc agcttgtatt
     1441 ttgctgaaga tcctcccaca tcactaaagt ccttcaggtg tccttctcct ctattcctgg
     1501 agaatgtacc aagtattcca gtggaagatg atgaaagcca gaataacaat ttactacctt
     1561 ctgatgaagg cctgaggata gacagaatga ctcagaaaac tccttttgaa tgcagccagt
     1621 ctgaggttat gtttctgagc ttggacaaaa agccagagag caagagaaat gaggaagctt
     1681 gcaacatgcc cagttcttct tgtgaagaaa gttggttccc aaagtatata gttccatccc
     1741 aggacttaag gccctataag gtaaatatag atccttcttc agaagctcca gggcattctt
     1801 gcaggagcag gccagtggag agcagctgtt cctccaaatt ttcctgggat gaatatgaac
     1861 agtacaaaaa agaataaatt ctaccagaag ataaagaaaa aagcaagtat tgcataggca
     1921 cctgagcata ggtatgacct tgggaagaca ttggctccat aagcaatgcc aagagaatga
     1981 tcaatagtga gtttgggtga tgcagataaa caatctggat aattccattt cttttttccc
     2041 aaaccctcaa acagagtgcc ttaaaaaatt gttttatcag gataattgtc tcatgaccaa
     2101 atccacgctc aattagagcc attcaaaatt ccttaagatc atgggttctg acttcagcca
     2161 aacaaaacaa tcaaaaccta ccaaaaaggg actggattgt aatgtcctct ccatcatcct
     2221 cagtgtgagt cctcagagcc tccatctgcc aagaacattc agttggattc catcgtttgg
     2281 tttagcttgc ttgcacgggt tgtaggaaat gtctaatttg taaatgttaa tagatacctt
     2341 tggaaagaat caccttgtta ttctgattga cccctcttgt tttttggtta atccctgact
     2401 agcctgcttg tctgaaaatg agccaggttc acccttagct acggcatgct ctgggttgaa
     2461 aggggatttc ttcccaagat ctatcctaaa ctcttaggac agtttatcct gtattgacta
     2521 ttattacagc tttttaaaaa cagacttagt gacctattat atcttaagga gactatgtga
     2581 aatgtccatc aagtaatttt tattcctgag aaaatggctg gtcaagggcc taggtcagct
     2641 ttttgtttaa tctacacctt tcaactctgg gccttcattc ctgctgtttc ttggatctcc
     2701 ctcttttgaa atcctaccaa tcatcagagc tctacaaaac agccacctcc tggaagcttc
     2761 cctaatctct acaaccagaa gtactccctt tatcagtatt tccacagcaa tttctttgta
     2821 tttctatttt atatttatca ctttctgaat tatatcaaag ctgcttgtct tatctctttt
     2881 tttttttgag acggagtctc gctctgtcat ccaggctgga gtgcagtggc acaatctcgg
     2941 ctcaccgcaa ggtctgcctc ccgggttcac gccattctcc tgcctcagcc tcccgagtaa
     3001 aggtgggact acaggcgccc gccactacgc ccggctaatt ttttgtattt ttagtagaga
     3061 cggggtttca ccatgttagg caggatggtc tcgatctcct gacctcatga tccgcctgcc
     3121 tggcctccca aagtgctggg attacaggcg tgagccaccg cgcccagcct tcatctcttt
     3181 tactaggttg taaactccct atattagttt tctattgttt ttgtaacaca ttatcacaaa
     3241 tttagtgact taaagtaaca taaattaatt attttgcagt tctggaggtc agaattcctt
     3301 agcattcttt ctggaggctg ttggggagaa ctcactgcct tgccttttcc agcttctaga
     3361 ggccacctgc cttctttggc tcatggcctc ttcctccgtc ttcaaagcca gcaacgtgac
     3421 atttcagatc tccctctctc tgtctgtctc accgcatctc ttcactaccc tcttcctctc
     3481 attctggccc ttctgcatgt cttttataaa gacctttgtg atttcactgg gcccactcat
     3541 ataagccagg aaaatctctg tgtctcagat ccttaattta attacacctg caaagtccgt
     3601 tttctcgtgt aaagtaacat attcacaggt ttctggaatt gggacgtgga catcttcggg
     3661 ggaccattac tctgtctgtc atactccctg agtgtgtaag ggccatctct tcagagccta
     3721 atgtgtcatc ctacacagag caggtgtttc cttataaatt aagttgaatt aaattacccc
     3781 tatgaagcca cataatcaat aattcagcta taataaacat tgccgaataa attaacaaag
     3841 ggaggataat gactccttat gatgatggat agaaacactg aagaaaacca ttttcagagg
     3901 atttactgaa ttttcctctc caaggagctt gtaatagaag ggtaaacctc tagtttcttg
     3961 tccagcagag gaataggctc atgtcttttg ttttatttta tttttttggt gagtcagtat
     4021 ggttgggtta atcatattca cattagattc catgttgaag aacaactaat caaagaagaa
     4081 aatcatcagg aatcagggtg agattaacag accaaggtca gaaacatgga gttacaattt
     4141 tccaaaaaag cacgaagtga gattaaaacc acagtaagtc ttagtctgac tgacaggctt
     4201 tatacaggcc ctcggtaagc agttgggaga acccatgccc accactgctc tgtacggtat
     4261 ctggctgctc agagctctct gattcacaaa agagatcatc aagaattgac tctgtgttaa
     4321 actgtttttc acttagaata tggaccccca ccaatacatt caatgaaggg tcattaagga
     4381 agctctgatt aacaccaggc ccatgcagtt actccgcaca tataatctgt gggtattatg
     4441 ttgatttgaa cttgcttagc tgacttaaag cagggtttct caaactgtag tatgcatcag
     4501 aatgacttgg aaagctggtt aaaaacgcag attgctgggt ccacccagct tctgatgcaa
     4561 taggtctggg gttagaaatt ttgctttcta atgagttccc aggtgatgct gatgccaatg
     4621 atgtgaagcc cgctcttcac atccgtgaaa atcgctgcct taaggtgctc ttaaattgtt
     4681 atagattggt agatcctacc tttccatcac tggtatgttt tttgagaaaa ggatcctttc
     4741 ttttcattct gttatattca tcctattaca taggatgttg gttagcacag agtgggagct
     4801 ctagaaagat tgttgaccaa tcatcttatt gactagacca tctttctaga gtataactat
     4861 tttggacacc tcaaagatga agtcagataa gctggcagtg acctgattaa cgttgtggta
     4921 agcagatgcc actgtggctg ggaagtacca aatcttctgt tgagtgattc agtcttcctg
     4981 actagatttg ccttctagtg ggtgtattaa taagcgttag ttgatatgaa ttaaatgttg
     5041 caactttcta tgggtaataa gaggatacct ttatagtttc atgatgtgga gaccaagata
     5101 cataagaaag caagaatgag tctccgggtg ttccctctga tcagcaaaca tgaattcata
     5161 atttcattta ctctcatttt tgtaatgcac tctgagcaat cctccaggca gatcctattt
     5221 actaagcttc aggcttttgt cccattttat aatgcccaaa cacatggaga aaacattttg
     5281 ttgtttttaa aaacaggtat gggatttctg atataatcaa ttttattgac ttcttgggag
     5341 ttgtaatgat ctgtgactca ctgcataata ttgtagtttt atagggtggc acatcaccct
     5401 gtgtaaaaca actttaatga cctgaggact gatgagtaca ggaagtgagg tttatcatga
     5461 gtagcattta ttaagcatct gctagaatca aagcactgca ttagctacta aggtacaaag
     5521 taagacacag tttaataagg aagacagggc atctacccaa gtcaacagtt cagtgacatc
     5581 tgttatttga accacacacc aatgcaaccg aagtaaatgc tgaaagggtg agaagaagga
     5641 atagattact atgcattttt gagaggatga agaaggcttc ttggagaagg tttctgaaaa
     5701 gagatacgga attaagagaa atagaaaaga gcactgacga agcactcagg atggctattc
     5761 aaggaattgg ggatccacag cagctgtgca caaccatccc taatgccaga aaaatggggt
     5821 aagggtgaag ggaactgggt ctagagcttt gcatgttttt ttctggttaa cccttagagt
     5881 caccctgaag gtgtggatcc catcttataa taaggtgagg acactgagtc aggaaactaa
     5941 cttgctaaaa tcaagtcctc ccagtcctcc catcaggtcc ttccagatct ggaggagcca
     6001 ggatttgaac tcacatttgt ctgacttcaa aatccatgct ttttcttgtg ttccaaattg
     6061 cacaatggag tcactgccag ttcaagctcc tatgaaaagg gatggatgtg aaaagtcatt
     6121 tgttgattcc acagattttt agtaggtacc atgtctcctg cctttatcac tatcctactc
     6181 ctctgctaca acttctctgc tacaggcacc cactgcagtt acttgatatg agttaaatga
     6241 atccatccat ccaaacttcc attcattgaa taaatactta gcaatggctt cctttgtatc
     6301 aggcactatt ctaggagctg gggctacagt gcagatggaa accagcaaag ctcactgccc
     6361 tcatggagct tatatgtaag tgaataaatc tcagggtggt tctcctgtca taggtcctgg
     6421 ctcctctgcc agctcttcca aatgggttca gagaggtttt ctccttgtcc tgttgtgtaa
     6481 ctgataatcc catggtggac ctcactttgc tattcatatg cactaagtag tttatggtgt
     6541 gctttgtggc tggcaattgt gacagattct attcttctat tggtatgtgg tttctttggg
     6601 gcatttgttt ggatactgat ggcatcttcc gggggtctga ggcacctaca caaacctcag
     6661 gtcactatca cgtgatactg gagttgcctg ttaaattata tttcttcttc cacaaaactg
     6721 caggttccct gagggaaagg tctatgtcct gctcattttt ggatccctaa tggctggcac
     6781 agtacctggt gcataatatg gttcaacaaa tatttgtaaa atgaatgaat caaatagtcc
     6841 ttcaaagcat cttcagaatc aagattgtca tctagattgc acccgaagtt tatctttcta
     6901 gtattcagaa tataatcaat cattgcttaa attattccat ttcatcattc ttgaataaaa
     6961 gctactaatt ttaaatacta atatctgaga tatcttcgaa aagaagatta gtcgttgtat
     7021 gtgttgctta taaattttct ctatggtgga aaatattccc aaaccatgtc taagcaatga
     7081 aaaaccagtg ttctggcagg aaataaactg atgtttgttg tgttttgtat ctgtcttccc
     7141 gttccaaatc atttatatat acttgatggt ctgtgggctt gacttgcaag tattttcctg
     7201 ggagacattt ttattcaaaa gacctgtatt gcctgccaaa ccttcattta agagactcat
     7261 tatggatgtc atctgcatca ttgcaaactt ttctggtcgt ctctataaat agcaagataa
     7321 tatttacaga gttttacatt ggctcttgaa ttttaaaata ttcttataga caaacattaa
     7381 ttggaatggg gggtaaggag gtaggaatgg cttacttgcg gaaatgcagg ctcattgttg
     7441 tgtctagtag gtgctaggtg cattcacaca tctcttaagc tttaaacaac ctattgagcc
     7501 ggtttccatt tccttgatct attagattta aaaaaatgaa gcataaagga gttggctctt
     7561 gccctaagtc atactactga tggaatgaga atacaaacca aaaacacttt attcaagtcc
     7621 aggaaatatc tgccttgaaa aagaataact taaaaattaa aagtgtcctc tatttgaaca
     7681 tgaaaacagc taataaagat gtgttatttt attttattat tattaatttt gtcctcccat
     7741 attggcaaag ataaagcaat tgacaaaaat ggaattcttg ttcaatactg gcaggagtga
     7801 aaattggtag aacctttcta gaaggcaatt tggcaacatg tatgaaaacc taaatgttga
     7861 tacaccttta cccagcagtt tgtttaggaa tttatcctaa tgaataaaag ttgtccaagt
     7921 cttcaaacat gagcccaaag gtatatttca tgatgtttat gatattaaaa cattggaaac
     7981 aactgaaaca tccttcagta aaagatggat taaataaatt ccatgcagtt gtcatttaaa
     8041 aatatttaga tatatgttta ttgctatgga tatatgttcc caaaatatta ttgaatcaaa
     8101 aagtagacta caggatatat gttgaatatg agctcattta taacattgaa tattttaaga
     8161 taatgtatgt ttcatagaga gatcttcacc aaatgttaag gatttttttt tctgggctgt
     8221 ggtatttggg tgatctttac attcttcaga ctcatgtgtg tttgaaactt tttataatga
     8281 acatatatca tttttattag aaaagaataa agtttttgaa aaaaaaaa
//
