LOCUS       NM_001134382            7700 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens IQ motif and Sec7 domain ArfGEF 1 (IQSEC1), transcript
            variant 1, mRNA.
ACCESSION   NM_001134382
VERSION     NM_001134382.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Zheng,X., Jing,J., Yuan,M., Liu,N. and Song,Y.
  TITLE     Contribution of gene polymorphisms on 3p25 to salivary gland
            carcinoma, ameloblastoma, and odontogenic keratocyst in the Chinese
            Han population
  JOURNAL   Oral Surg Oral Med Oral Pathol Oral Radiol 136 (2), 220-230 (2023)
   PUBMED   37495273
  REMARK    GeneRIF: Contribution of gene polymorphisms on 3p25 to salivary
            gland carcinoma, ameloblastoma, and odontogenic keratocyst in the
            Chinese Han population.
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Fan,L., Yang,K., Yu,R., Hui,H. and Wu,W.
  TITLE     circ-Iqsec1 induces bone marrow-derived mesenchymal stem cell
            (BMSC) osteogenic differentiation through the miR-187-3p/Satb2
            signaling pathway
  JOURNAL   Arthritis Res Ther 24 (1), 273 (2022)
   PUBMED   36517907
  REMARK    GeneRIF: circ-Iqsec1 induces bone marrow-derived mesenchymal stem
            cell (BMSC) osteogenic differentiation through the miR-187-3p/Satb2
            signaling pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7700)
  AUTHORS   Nacke,M., Sandilands,E., Nikolatou,K., Roman-Fernandez,A.,
            Mason,S., Patel,R., Lilla,S., Yelland,T., Galbraith,L.C.A.,
            Freckmann,E.C., McGarry,L., Morton,J.P., Shanks,E., Leung,H.Y.,
            Markert,E., Ismail,S., Zanivan,S., Blyth,K. and Bryant,D.M.
  TITLE     An ARF GTPase module promoting invasion and metastasis through
            regulating phosphoinositide metabolism
  JOURNAL   Nat Commun 12 (1), 1623 (2021)
   PUBMED   33712589
  REMARK    GeneRIF: An ARF GTPase module promoting invasion and metastasis
            through regulating phosphoinositide metabolism.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7700)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 7700)
  AUTHORS   D'Souza,R.S., Lim,J.Y., Turgut,A., Servage,K., Zhang,J., Orth,K.,
            Sosale,N.G., Lazzara,M.J., Allegood,J. and Casanova,J.E.
  TITLE     Calcium-stimulated disassembly of focal adhesions mediated by an
            ORP3/IQSec1 complex
  JOURNAL   Elife 9, e54113 (2020)
   PUBMED   32234213
  REMARK    GeneRIF: Calcium-stimulated disassembly of focal adhesions mediated
            by an ORP3/IQSec1 complex.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7700)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 7700)
  AUTHORS   Morishige,M., Hashimoto,S., Ogawa,E., Toda,Y., Kotani,H.,
            Hirose,M., Wei,S., Hashimoto,A., Yamada,A., Yano,H., Mazaki,Y.,
            Kodama,H., Nio,Y., Manabe,T., Wada,H., Kobayashi,H. and Sabe,H.
  TITLE     GEP100 links epidermal growth factor receptor signalling to Arf6
            activation to induce breast cancer invasion
  JOURNAL   Nat Cell Biol 10 (1), 85-92 (2008)
   PUBMED   18084281
  REMARK    GeneRIF: Results indicate that GEP100 links EGFR signalling to Arf6
            activation to induce invasive activities of some breast cancer
            cells, and hence may contribute to their metastasis and malignancy.
REFERENCE   8  (bases 1 to 7700)
  AUTHORS   Hiroi,T., Someya,A., Thompson,W., Moss,J. and Vaughan,M.
  TITLE     GEP100/BRAG2: activator of ADP-ribosylation factor 6 for regulation
            of cell adhesion and actin cytoskeleton via E-cadherin and
            alpha-catenin
  JOURNAL   Proc Natl Acad Sci U S A 103 (28), 10672-10677 (2006)
   PUBMED   16807291
  REMARK    GeneRIF: Guanine nucleotide-exchange protein (GEP) 100/BRAG2 has an
            important role in the activation of ADP-ribosylation factor (ARF)6
            for its functions in both E-cadherin recycling and actin
            remodeling.
REFERENCE   9  (bases 1 to 7700)
  AUTHORS   Dunphy,J.L., Moravec,R., Ly,K., Lasell,T.K., Melancon,P. and
            Casanova,J.E.
  TITLE     The Arf6 GEF GEP100/BRAG2 regulates cell adhesion by controlling
            endocytosis of beta1 integrins
  JOURNAL   Curr Biol 16 (3), 315-320 (2006)
   PUBMED   16461286
  REMARK    GeneRIF: Arf6 regulates both endocytosis and recycling of beta1
            integrins and BRAG2 (IQSEC1) functions selectively to activate Arf6
            during integrin internalization
REFERENCE   10 (bases 1 to 7700)
  AUTHORS   Someya,A., Sata,M., Takeda,K., Pacheco-Rodriguez,G., Ferrans,V.J.,
            Moss,J. and Vaughan,M.
  TITLE     ARF-GEP(100), a guanine nucleotide-exchange protein for
            ADP-ribosylation factor 6
  JOURNAL   Proc Natl Acad Sci U S A 98 (5), 2413-2418 (2001)
   PUBMED   11226253
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC069271.5, AK294525.1, AY653734.1 and AC018836.5.
            
            On Nov 22, 2018 this sequence version replaced NM_001134382.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK294525.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000613206.2/ ENSP00000480301.1
            RefSeq Select criteria :: based on computational evidence
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-312               AC069271.5         84649-84960
            313-1691            AK294525.1         1-1379
            1692-2309           AY653734.1         1328-1945
            2310-3894           AK294525.1         1998-3582
            3895-7700           AC018836.5         59146-62951
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.2-p25.1"
     gene            1..7700
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /note="IQ motif and Sec7 domain ArfGEF 1"
                     /db_xref="GeneID:9922"
                     /db_xref="HGNC:HGNC:29112"
                     /db_xref="MIM:610166"
     exon            1..438
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     CDS             416..3760
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /note="isoform a is encoded by transcript variant 1; IQ
                     motif and SEC7 domain-containing protein 1;
                     brefeldin-resistant Arf-GEF 2 protein; ADP-ribosylation
                     factors guanine nucleotide-exchange protein 2;
                     ADP-ribosylation factors guanine nucleotide-exchange
                     protein 100; brefeldin A-resistant ARF-GEF2; epididymis
                     secretory sperm binding protein; IQ motif and Sec7 domain
                     1"
                     /codon_start=1
                     /product="IQ motif and SEC7 domain-containing protein 1
                     isoform a"
                     /protein_id="NP_001127854.1"
                     /db_xref="CCDS:CCDS74902.1"
                     /db_xref="GeneID:9922"
                     /db_xref="HGNC:HGNC:29112"
                     /db_xref="MIM:610166"
                     /translation="MACRRRYFVEGEAPSSETGTSLDSPSAYPQGPLVPGSSLSPDHY
                     EHTSVGAYGLYSGPPGQQQRTRRPKLQHSTSILRKQAEEEAIKRSRSLSESYELSSDL
                     QDKQVEMLERKYGGRLVTRHAARTIQTAFRQYQMNKNFERLRSSMSENRMSRRIVLSN
                     MRMQFSFEGPEKVHSSYFEGKQVSVTNDGSQLGALVSPECGDLSEPTTLKSPAPSSDF
                     ADAITELEDAFSRQVKSLAESIDDALNCRSLHTEEAPALDAARARDTEPQTALHGMDH
                     RKLDEMTASYSDVTLYIDEEELSPPLPLSQAGDRPSSTESDLRLRAGGAAPDYWALAH
                     KEDKADTDTSCRSTPSLERQEQRLRVEHLPLLTIEPPSDSSVDLSDRSERGSLKRQSA
                     YERSLGGQQGSPKHGPHSGAPKSLPREEPELRPRPPRPLDSHLAINGSANRQSKSESD
                     YSDGDNDSINSTSNSNDTINCSSESSSRDSLREQTLSKQTYHKEARNSWDSPAFSNDV
                     IRKRHYRIGLNLFNKKPEKGVQYLIERGFVPDTPVGVAHFLLQRKGLSRQMIGEFLGN
                     RQKQFNRDVLDCVVDEMDFSTMELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCIC
                     NPGVVRQFRNPDTIFILAFAIILLNTDMYSPNVKPERKMKLEDFIKNLRGVDDGEDIP
                     REMLMGIYERIRKRELKTNEDHVSQVQKVEKLIVGKKPIGSLHPGLGCVLSLPHRRLV
                     CYCRLFEVPDPNKPQKLGLHQREIFLFNDLLVVTKIFQKKKNSVTYSFRQSFSLYGMQ
                     VLLFENQYYPNGIRLTSSVPGADIKVLINFNAPNPQDRKKFTDDLRESIAEVQEMEKH
                     RIESELEKQKGVVRPSMSQCSSLKKESGNGTLSRACLDDSYASGEGLKRSALSSSLRD
                     LSEAGKRGRRSSAGSLESNVEGSIISSPHMRRRATSTRECPSRPHQTMPNSSSLLGSL
                     FGSKRGKPPPQAHLPSAPALPPPHPPVVLPHLQHSVAGHHLGPPEGLPQAAMHGHHTQ
                     YCHMQNPPPYHHHHHHHPPQHIQHAHQYHHGPHGGHPAYGAHAHGHPPLPSAHVGHTV
                     HHHGQPPAPPPPTSSKAKPSGISTIV"
     exon            439..733
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            734..1983
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2145
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2146..2268
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..2435
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2436..2575
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2576..2605
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2606..2731
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2732..2831
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2832..2993
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            2994..3170
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            3171..3220
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     exon            3221..7700
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6842..6847
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /note="hexamer: AATAAA"
     polyA_site      6869
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /note="major polyA site"
     regulatory      7504..7509
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /note="hexamer: AATAAA"
     polyA_site      7522
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /note="major polyA site"
     regulatory      7677..7682
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
                     /note="hexamer: AATAAA"
     polyA_site      7700
                     /gene="IQSEC1"
                     /gene_synonym="ARF-GEP100; ARFGEP100; BRAG2; GEP100;
                     IDDSSBA"
ORIGIN      
        1 ggatgctgcg agagcaaacc cggggcagcc ttctctcgac cctcccctcc tcatccccgc
       61 gccccgcacc ccggatggac gggagggaaa aaccccaagg tgggcccagg tagcagcgag
      121 gtggacgccc ccgcccagcc tggcgaccac ggttactcga cgtcctcgct cgctcctggg
      181 cacggtgtga gcgcgcgcta cgtgccaggg ccctccacag cccgctgctg gcgccgcccc
      241 ccgctcccgc cccagcctcc ctggaggagc ccgccacccc cgggaatgtg actcgccccg
      301 cgatttaatt ttattcccct ccacttcttg cctgagccgc ctgctcctct tggaaacacg
      361 ttgagcctcc ccgctggaga gggagccaga acagggaaga acggattcac acaggatggc
      421 ttgcagaaga cgctatttcg tcgagggcga ggcccccagc agtgagactg gcacatccct
      481 ggacagcccc tcagcctacc cccagggccc cttggtgccc ggttccagcc tgagcccgga
      541 tcactacgag cacacgtcag tgggagccta tgggctgtac tcggggccgc cggggcaaca
      601 gcagcgcacg cggaggccca agctgcagca ctcgacctcc atcctgcgca agcaggctga
      661 ggaggaggcc atcaagcgct cacgctcact ctccgagagc tatgagctct cctcggacct
      721 gcaggacaag caggtggaga tgctagaacg aaagtatggg gggcgcctgg taacccgcca
      781 tgcggcccgc accatccaga cggcgtttcg ccagtaccag atgaacaaga acttcgagcg
      841 cttgcgcagc tccatgtcag agaaccgcat gtcacgccgg attgtgctgt ccaacatgag
      901 gatgcagttc tcctttgagg ggcctgagaa agtgcacagc tcctacttcg aggggaagca
      961 ggtctcagtg actaacgacg gctcccagct gggagccctg gtgtcccctg agtgtggtga
     1021 cctcagcgag cccaccaccc tcaagtctcc ggccccctcc agtgactttg cggacgccat
     1081 caccgagctg gaggacgcct tctctaggca agtgaaatca ctggccgagt ccatcgacga
     1141 tgccctcaac tgccgcagcc tgcacactga ggaggcaccg gccctggatg cggcgcgggc
     1201 ccgggacacc gaaccccaga cagccctgca cggcatggac caccgcaaac tggacgagat
     1261 gacggcctcg tacagtgatg tcaccctgta catcgatgag gaggagctgt cgccccctct
     1321 gcccctctcg caggcagggg accggccgtc cagcaccgag tcggacctgc ggctacgggc
     1381 tgggggcgca gccccagact actgggccct ggcccacaaa gaggacaagg ctgacacgga
     1441 cacgagctgc cggagcacgc cgtcgctgga gcggcaggag cagcggctgc gggtggagca
     1501 tctgccgctg ctcaccatcg agccacccag cgacagctct gtggacctta gtgaccgctc
     1561 ggagcggggg tcactcaaga ggcagagtgc ttacgagcgc agccttggcg ggcagcaggg
     1621 cagtcccaag catggtcccc acagcggcgc ccccaagagc ctcccccggg aggagcctga
     1681 gttgcggccc cggcccccca ggcccctgga cagccacttg gccatcaatg gctcagccaa
     1741 ccggcagagc aagtctgagt cggactactc agacggtgac aatgacagca tcaacagcac
     1801 gtccaactcc aacgatacca tcaactgcag ctccgagtca tcgtcccgtg acagcctgcg
     1861 ggagcagacg ctcagcaagc agacctacca caaggaggcc cgcaacagct gggactcgcc
     1921 tgcctttagc aacgatgtca tccgcaagag gcactaccgc atcggcctga acctcttcaa
     1981 caagaagcct gagaagggag tccagtacct catcgagcgt ggctttgtgc ccgacacgcc
     2041 cgtcggggtg gcccacttcc tgctgcagcg caagggcctc agccggcaga tgatcggcga
     2101 gttcctgggc aaccggcaga agcagttcaa ccgtgacgtg ctcgactgcg tcgtggacga
     2161 gatggacttc tctaccatgg agctggatga ggccctcagg aaattccagg cgcacatccg
     2221 tgtccaaggg gaggctcaga aagtggagcg gctcatagag gcgttcagcc agcgctactg
     2281 catctgcaac cctggggtgg tgcggcaatt ccggaaccca gacaccattt tcatcctggc
     2341 cttcgccatc atcctgctga acaccgacat gtacagcccc aatgtcaagc ccgagcggaa
     2401 aatgaagcta gaggacttca tcaagaacct ccgaggtgtg gacgatggtg aggacattcc
     2461 ccgtgagatg ctgatgggga tctatgaacg gatccgtaag cgagagctaa agaccaatga
     2521 ggaccatgtg tcccaggtgc agaaggtgga gaagctcatt gtggggaaaa agccgatcgg
     2581 atccctgcat cccgggctcg gctgtgtgct ctctctgccc caccgtcggt tggtctgcta
     2641 ctgccggctc tttgaggttc cagacccaaa caagccccag aaactcggac tacaccagcg
     2701 agaaatcttc ctgttcaacg acctcctggt ggtcaccaag atcttccaga agaagaagaa
     2761 ctcggtgacg tacagcttcc gacagtcctt ctccttgtac ggcatgcagg tcctgctctt
     2821 cgagaaccag tactacccca atggcatccg gctcacctcg tctgtccccg gagcagatat
     2881 caaagtgtta ataaacttca acgcccccaa ccctcaagac cggaagaaat tcaccgatga
     2941 cctgcgggag tccattgcgg aagtccaaga gatggagaag cacaggatag agtcggagct
     3001 cgagaagcag aaaggcgtcg tgcggcccag catgtcccag tgctctagcc tcaaaaagga
     3061 gtcgggcaac ggaacactga gccgggcctg cctggacgac agctatgcca gcggtgaggg
     3121 cctcaagcgc agcgccctca gcagctccct gcgggacctc tcggaagccg ggaagcgagg
     3181 gcgtcgcagc agtgcgggat cgctagagag caatgtggaa gggtccatca ttagcagtcc
     3241 tcacatgcgc cggagagcta catcaacacg agagtgtcca tctcgcccac accagactat
     3301 gcccaactca tcttccctcc tgggctcctt attcgggagc aagagaggga agccccctcc
     3361 ccaggcccac ctgccctcag ccccagccct gccacccccc cacccaccgg tggtcctgcc
     3421 tcacttgcag cactctgtgg ctggccacca cctggggccc ccagaggggc tgccgcaggc
     3481 cgccatgcac gggcatcaca cccagtactg ccacatgcag aaccctcccc cgtaccacca
     3541 tcaccaccac caccacccac cccagcacat ccagcacgca caccagtacc accacggccc
     3601 ccatgggggc cacccagcct acggggccca tgcccacggc cacccgccgc tgccctcggc
     3661 ccacgtgggg cacacagtgc accaccatgg gcagccccct gccccgccgc cccccaccag
     3721 cagcaaggcc aaacccagcg gcatcagcac aattgtgtag acagcctggg taggggtccc
     3781 aggctccctg aaacacctgc acaccacaca gggcacgccc gggggtcgcc agccgcacac
     3841 caaacccggg gcacttctgt tgccatctct cccctctgcc cctcacggcc caaccggagc
     3901 cccaggagcc cacagggctg gtgttgtgtg gaacaaaggc ccagatttca tttcttgttg
     3961 gcaccctggg ctctgctcac ctcagtctga gggatgggtg ggcctcagac accatcagcc
     4021 ttgaaacggt gagccagcca agtagtgttg aactgcctcc cccactccag ctctcagctc
     4081 cctgtgccct aatgtacatg catatgaaaa cccaacctag aaaacgaaga aatgagatac
     4141 aaaaacagac aaaacaaacc ccaaaacttg ctgcattatt gctcttttat tgacaatggg
     4201 caaaaaaata agtagacctg atatggttga tgaaaatacg taagtaaact ttatataaat
     4261 atataaatat ataaatatat atatatatat actgtatagg tagtacttgt gtgtgaaagg
     4321 caggcgtttc agtccacatt agcaataccc accttacaag gagctccact tacctaatag
     4381 gaagacagta ccttagctgg gtgtgtgaga caatagacca aaccctaaat gctaggaaca
     4441 aattcagata cttcatattt tcatacaaag aagtccctct aggactggct aaaatcttta
     4501 cacaatcatt actaactgtg ccaagtaaca tagcatctaa ctgtttaaaa agtccagtat
     4561 tgctttgtat aaatccttat tttattaaca gaatactatc ataaatagta ttataatgct
     4621 gttatttcag gtaagcaaat agctaaactg cagtacactc tacagtagca actcaggaca
     4681 gctggttaca agctggttgt cttaggacat tggttacacg gattcttaga cactttaatg
     4741 gctgcgataa ctgtgactct ccatgatcca tgtttctttt atgcgcatat gatttgacgc
     4801 acactcattc agagtcctcc gagaggggca cccatacacg gcagaagtgt tcatctccaa
     4861 catgaaagtg accagctctc atcctcgtct ccccaacacc ataacgtcct catcccgcct
     4921 ccaacccaca ccaggccgaa gccctcagag agtgttttca tcaggaacca ctctcgaacc
     4981 tgaaggttga ctttagcgtt tagcaaccca gggcggtgtg tgtgtttccc gttttgtttt
     5041 ctgagtggta gcagtgatca ccgtaattcc atgtagccat gtgctagcag aacccctgtg
     5101 tcctcaccgt ggcccgtgtg accccagccg acgagtgccc ggcggagccc ccgctgcctt
     5161 cccatggtcc agtgagctgc cagggcatca catgactctc agctgtgctc ttgtcgcttc
     5221 tgtgttgtgg tgacaccatg cgctccccag ggccagacct gcacgcggca ggtctgtgcc
     5281 cgagtcaccc acgggccata ctttgtagtt tcagcctttc gagccactgc agccgtcagt
     5341 gctgtgctcc taagccatgg gacccgagga ctgccccccg gcgcctgccc aggcggaggc
     5401 cctttcagaa agggcgaagc tcacgcctga ctctgcgggc cgcggggccg cgttcccagt
     5461 ggacagcgtg gtgagccgtg gccggacggc aggaggagag gggagccccc tctggctgtg
     5521 tgtcaccttg gctggctggc tggccagggt tttgccgatt tccctcctca catccctccc
     5581 accctcggtc atatcattga gatcaccgta ccagcaaatc tgcaaaccaa gcactttgtt
     5641 aatctccaga gagagcaaat acagcaatct agagtttagc ctttttaaaa atgtgacttt
     5701 aacccgccca cctgtgtcct cccattggcg tggcatttct ttggtgtctg ctcagaaaga
     5761 aacagcatgg gaccgtgtga ttgggtccag gctgggctgt cagggaagcg ccctggtgtc
     5821 tccgactcat caattctcgg gtctttttta aaagccaggc tccccaaagc actctgtctc
     5881 gcaattttga ggaggctgtt ccgacctgga agcgttaaac tgctgcatgt catctggcgt
     5941 cagctgtgag gctgcgctct gcgtgttgtg cgtttgcaaa atgtattggc gtgactgtaa
     6001 acacatcgtt gaagtaaagc gattacatat tgagtatgtc agcttttctc accctcttct
     6061 gtgctgggag gagagtctaa ctgtaacctt taggttgttc cagaaaagat tcaggccgtt
     6121 tggcacagaa ttgctctttc tgaagggtag cagtaatgaa taggaacaga cagaaaaccc
     6181 caggaggggc tttcaggaaa ctctccctct ccatcgccat cctgcaagct ccacagcctc
     6241 gggctgccag cctgtggcac tgtcctcggt gtgtcctgga gctgtgctgg gcagcagggc
     6301 ctcgcgtaag gaactgctgg aacccactga gcccttcccg gggtttcccg ccgcactcag
     6361 tgtgttcctt caggctagtg aggtttcatt ctgttttatt tgcaagctta tcaaaagttt
     6421 gtggaagata cattgttggg ttccagaggc tggagtcatg ctgcccacat cttgtttggg
     6481 aaagcaaaag ctcccagtga gctggtgtcc ccgtgtgtct ttcatgggac tgtccccttt
     6541 agagatccca cctgtcagag ccacagcttc ggggtcctgt catcacatcc cctgggaggg
     6601 gaggcaggag gaggccgagg gccccaggat gtctgcctgg tcccttctca ctgtgaggac
     6661 gcccttggca cacctttctc aagtcacaca aattactgca gttacataca gaattgctga
     6721 caggagcagg agacgctgag ggaaacagct ggcaatgtga caaaagtctt ttctgggaca
     6781 acaatcaaat catgtatttg tattttttta agtttaccaa tgaattgtac aacaatgtaa
     6841 aaataaagtt catcctaata tgctgtgcac atcttgctta aaaatgtctt cagcacccag
     6901 tgcagtgatc tttccctcac acactgcatg cagcaggagc ctcttgtttt tcttaatgag
     6961 ctgaaaagac agcagatgtt cacagctgat gctgggtgtt gactcagcgc cacgggaagc
     7021 tctgtgcccg caggtaagca cgcacagtct tacagttctc cttgtaaaat atcatcaccc
     7081 ttcctgcctg agaaacgagt acatttgtat tttatgtaac aacaatagtc aaggcagcat
     7141 tgcaacactg agcagggtga ggtgggggtg ctgagcacag gacaagaggg aggaggcctg
     7201 ccctccattc agatgcatct gcatcgccct tctgtgtgca gtcacatcga gactgcacac
     7261 agcatgcatc tcctcttgga aaatatcttt tgcctccgag ttgttttaag caattagaat
     7321 caggtcctga atccccagac tgttcagaga cttcaaggga cacctccctc acaagaagcc
     7381 tggcaccccc actgtgtacc tttgagagga aaggagtgcg tttgttttca tctgcattct
     7441 gtttgtcagt ggcaacagac tctatcaatg taaaaccatg gttgtgatca tgtggggaaa
     7501 tcaaataaat cctttgaaac tctcggattc tcactttttc tgaaaagtcc agaagttgcc
     7561 ctgttggcca gacccagatc tccagctgat gagtttcccc attacttgtt gtgactctgg
     7621 gcactcactt tccttgaaga cattggactg actgaggaca ctgatacaaa atttaaaata
     7681 aagggacaca tcacaaccaa
//
