LOCUS       NM_022755               4268 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens inositol-pentakisphosphate 2-kinase (IPPK), mRNA.
ACCESSION   NM_022755
VERSION     NM_022755.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Looker,H.C., Merchant,M.L., Rane,M.J., Nelson,R.G., Kimmel,P.L.,
            Rovin,B.H., Klein,J.B. and Mauer,M.
  CONSRTM   CKD Biomarkers Consortium
  TITLE     Urine inositol pentakisphosphate 2-kinase and changes in kidney
            structure in early diabetic kidney disease in type 1 diabetes
  JOURNAL   Am J Physiol Renal Physiol 315 (5), F1484-F1492 (2018)
   PUBMED   30132343
  REMARK    GeneRIF: Higher urine IPP2K/Cr is associated with less severe
            kidney lesions at baseline and with preservation of kidney
            structure over 5 yr in individuals with type 1 diabetes and no
            clinical evidence of DKD at baseline.
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Okamura,M., Yamanaka,Y., Shigemoto,M., Kitadani,Y., Kobayashi,Y.,
            Kambe,T., Nagao,M., Kobayashi,I., Okumura,K. and Masuda,S.
  TITLE     Depletion of mRNA export regulator DBP5/DDX19, GLE1 or IPPK that is
            a key enzyme for the production of IP6, resulting in differentially
            altered cytoplasmic mRNA expression and specific cell defect
  JOURNAL   PLoS One 13 (5), e0197165 (2018)
   PUBMED   29746542
  REMARK    GeneRIF: These results imply that DBP5, GLE1 and IP6 have a
            conserved and individual function in the cytoplasmic mRNA
            expression. Variations in phenotype are due to the difference in
            each function of DBP5, GLE1 and IPPK in intracellular mRNA
            metabolism.
            Erratum:[PLoS One. 2019 Jul 25;14(7):e0220511. doi:
            10.1371/journal.pone.0220511.. PMID: 31344122]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4268)
  AUTHORS   Scherer,P.C., Ding,Y., Liu,Z., Xu,J., Mao,H., Barrow,J.C., Wei,N.,
            Zheng,N., Snyder,S.H. and Rao,F.
  TITLE     Inositol hexakisphosphate (IP6) generated by IP5K mediates
            cullin-COP9 signalosome interactions and CRL function
  JOURNAL   Proc Natl Acad Sci U S A 113 (13), 3503-3508 (2016)
   PUBMED   26976604
  REMARK    GeneRIF: IP6 synthase, Ins(1,3,4,5,6)P5 2-kinase (IPPK/IP5K) binds
            to cullins. Depleting IP5K increases the percentage of neddylated,
            active Cul1 and Cul4A, and decreases levels of the Cul1/4A
            substrates p27 and p21.
REFERENCE   4  (bases 1 to 4268)
  AUTHORS   Gosein,V. and Miller,G.J.
  TITLE     Conformational stability of inositol 1,3,4,5,6-pentakisphosphate
            2-kinase (IPK1) dictates its substrate selectivity
  JOURNAL   J Biol Chem 288 (52), 36788-36795 (2013)
   PUBMED   24165122
  REMARK    GeneRIF: IPK1 substrate selection is linked to the ability of each
            potential substrate to stabilize IPK1.
REFERENCE   5  (bases 1 to 4268)
  AUTHORS   Brehm,M.A., Wundenberg,T., Williams,J., Mayr,G.W. and Shears,S.B.
  TITLE     A non-catalytic role for inositol 1,3,4,5,6-pentakisphosphate
            2-kinase in the synthesis of ribosomal RNA
  JOURNAL   J Cell Sci 126 (Pt 2), 437-444 (2013)
   PUBMED   23203802
REFERENCE   6  (bases 1 to 4268)
  AUTHORS   Lango Allen,H., Estrada,K., Lettre,G., Berndt,S.I., Weedon,M.N.,
            Rivadeneira,F., Willer,C.J., Jackson,A.U., Vedantam,S.,
            Raychaudhuri,S., Ferreira,T., Wood,A.R., Weyant,R.J., Segre,A.V.,
            Speliotes,E.K., Wheeler,E., Soranzo,N., Park,J.H., Yang,J.,
            Gudbjartsson,D., Heard-Costa,N.L., Randall,J.C., Qi,L., Vernon
            Smith,A., Magi,R., Pastinen,T., Liang,L., Heid,I.M., Luan,J.,
            Thorleifsson,G., Winkler,T.W., Goddard,M.E., Sin Lo,K., Palmer,C.,
            Workalemahu,T., Aulchenko,Y.S., Johansson,A., Zillikens,M.C.,
            Feitosa,M.F., Esko,T., Johnson,T., Ketkar,S., Kraft,P., Mangino,M.,
            Prokopenko,I., Absher,D., Albrecht,E., Ernst,F., Glazer,N.L.,
            Hayward,C., Hottenga,J.J., Jacobs,K.B., Knowles,J.W., Kutalik,Z.,
            Monda,K.L., Polasek,O., Preuss,M., Rayner,N.W., Robertson,N.R.,
            Steinthorsdottir,V., Tyrer,J.P., Voight,B.F., Wiklund,F., Xu,J.,
            Zhao,J.H., Nyholt,D.R., Pellikka,N., Perola,M., Perry,J.R.,
            Surakka,I., Tammesoo,M.L., Altmaier,E.L., Amin,N., Aspelund,T.,
            Bhangale,T., Boucher,G., Chasman,D.I., Chen,C., Coin,L.,
            Cooper,M.N., Dixon,A.L., Gibson,Q., Grundberg,E., Hao,K., Juhani
            Junttila,M., Kaplan,L.M., Kettunen,J., Konig,I.R., Kwan,T.,
            Lawrence,R.W., Levinson,D.F., Lorentzon,M., McKnight,B.,
            Morris,A.P., Muller,M., Suh Ngwa,J., Purcell,S., Rafelt,S.,
            Salem,R.M., Salvi,E., Sanna,S., Shi,J., Sovio,U., Thompson,J.R.,
            Turchin,M.C., Vandenput,L., Verlaan,D.J., Vitart,V., White,C.C.,
            Ziegler,A., Almgren,P., Balmforth,A.J., Campbell,H., Citterio,L.,
            De Grandi,A., Dominiczak,A., Duan,J., Elliott,P., Elosua,R.,
            Eriksson,J.G., Freimer,N.B., Geus,E.J., Glorioso,N., Haiqing,S.,
            Hartikainen,A.L., Havulinna,A.S., Hicks,A.A., Hui,J., Igl,W.,
            Illig,T., Jula,A., Kajantie,E., Kilpelainen,T.O., Koiranen,M.,
            Kolcic,I., Koskinen,S., Kovacs,P., Laitinen,J., Liu,J., Lokki,M.L.,
            Marusic,A., Maschio,A., Meitinger,T., Mulas,A., Pare,G.,
            Parker,A.N., Peden,J.F., Petersmann,A., Pichler,I.,
            Pietilainen,K.H., Pouta,A., Ridderstrale,M., Rotter,J.I.,
            Sambrook,J.G., Sanders,A.R., Schmidt,C.O., Sinisalo,J., Smit,J.H.,
            Stringham,H.M., Bragi Walters,G., Widen,E., Wild,S.H.,
            Willemsen,G., Zagato,L., Zgaga,L., Zitting,P., Alavere,H.,
            Farrall,M., McArdle,W.L., Nelis,M., Peters,M.J., Ripatti,S., van
            Meurs,J.B., Aben,K.K., Ardlie,K.G., Beckmann,J.S., Beilby,J.P.,
            Bergman,R.N., Bergmann,S., Collins,F.S., Cusi,D., den Heijer,M.,
            Eiriksdottir,G., Gejman,P.V., Hall,A.S., Hamsten,A., Huikuri,H.V.,
            Iribarren,C., Kahonen,M., Kaprio,J., Kathiresan,S., Kiemeney,L.,
            Kocher,T., Launer,L.J., Lehtimaki,T., Melander,O., Mosley,T.H. Jr.,
            Musk,A.W., Nieminen,M.S., O'Donnell,C.J., Ohlsson,C., Oostra,B.,
            Palmer,L.J., Raitakari,O., Ridker,P.M., Rioux,J.D., Rissanen,A.,
            Rivolta,C., Schunkert,H., Shuldiner,A.R., Siscovick,D.S.,
            Stumvoll,M., Tonjes,A., Tuomilehto,J., van Ommen,G.J., Viikari,J.,
            Heath,A.C., Martin,N.G., Montgomery,G.W., Province,M.A., Kayser,M.,
            Arnold,A.M., Atwood,L.D., Boerwinkle,E., Chanock,S.J., Deloukas,P.,
            Gieger,C., Gronberg,H., Hall,P., Hattersley,A.T., Hengstenberg,C.,
            Hoffman,W., Lathrop,G.M., Salomaa,V., Schreiber,S., Uda,M.,
            Waterworth,D., Wright,A.F., Assimes,T.L., Barroso,I., Hofman,A.,
            Mohlke,K.L., Boomsma,D.I., Caulfield,M.J., Cupples,L.A.,
            Erdmann,J., Fox,C.S., Gudnason,V., Gyllensten,U., Harris,T.B.,
            Hayes,R.B., Jarvelin,M.R., Mooser,V., Munroe,P.B., Ouwehand,W.H.,
            Penninx,B.W., Pramstaller,P.P., Quertermous,T., Rudan,I.,
            Samani,N.J., Spector,T.D., Volzke,H., Watkins,H., Wilson,J.F.,
            Groop,L.C., Haritunians,T., Hu,F.B., Kaplan,R.C., Metspalu,A.,
            North,K.E., Schlessinger,D., Wareham,N.J., Hunter,D.J.,
            O'Connell,J.R., Strachan,D.P., Wichmann,H.E., Borecki,I.B., van
            Duijn,C.M., Schadt,E.E., Thorsteinsdottir,U., Peltonen,L.,
            Uitterlinden,A.G., Visscher,P.M., Chatterjee,N., Loos,R.J.,
            Boehnke,M., McCarthy,M.I., Ingelsson,E., Lindgren,C.M.,
            Abecasis,G.R., Stefansson,K., Frayling,T.M. and Hirschhorn,J.N.
  TITLE     Hundreds of variants clustered in genomic loci and biological
            pathways affect human height
  JOURNAL   Nature 467 (7317), 832-838 (2010)
   PUBMED   20881960
REFERENCE   7  (bases 1 to 4268)
  AUTHORS   Deleu,S., Choi,K., Pesesse,X., Cho,J., Sulis,M.L., Parsons,R. and
            Shears,S.B.
  TITLE     Physiological levels of PTEN control the size of the cellular
            Ins(1,3,4,5,6)P(5) pool
  JOURNAL   Cell Signal 18 (4), 488-498 (2006)
   PUBMED   15979280
REFERENCE   8  (bases 1 to 4268)
  AUTHORS   Verbsky,J. and Majerus,P.W.
  TITLE     Increased levels of inositol hexakisphosphate (InsP6) protect
            HEK293 cells from tumor necrosis factor (alpha)- and Fas-induced
            apoptosis
  JOURNAL   J Biol Chem 280 (32), 29263-29268 (2005)
   PUBMED   15967797
REFERENCE   9  (bases 1 to 4268)
  AUTHORS   Humphray,S.J., Oliver,K., Hunt,A.R., Plumb,R.W., Loveland,J.E.,
            Howe,K.L., Andrews,T.D., Searle,S., Hunt,S.E., Scott,C.E.,
            Jones,M.C., Ainscough,R., Almeida,J.P., Ambrose,K.D., Ashwell,R.I.,
            Babbage,A.K., Babbage,S., Bagguley,C.L., Bailey,J., Banerjee,R.,
            Barker,D.J., Barlow,K.F., Bates,K., Beasley,H., Beasley,O.,
            Bird,C.P., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.,
            Burrill,W., Burton,J., Carder,C., Carter,N.P., Chapman,J.C.,
            Chen,Y., Clarke,G., Clark,S.Y., Clee,C.M., Clegg,S., Collier,R.E.,
            Corby,N., Crosier,M., Cummings,A.T., Davies,J., Dhami,P., Dunn,M.,
            Dutta,I., Dyer,L.W., Earthrowl,M.E., Faulkner,L., Fleming,C.J.,
            Frankish,A., Frankland,J.A., French,L., Fricker,D.G., Garner,P.,
            Garnett,J., Ghori,J., Gilbert,J.G., Glison,C., Grafham,D.V.,
            Gribble,S., Griffiths,C., Griffiths-Jones,S., Grocock,R., Guy,J.,
            Hall,R.E., Hammond,S., Harley,J.L., Harrison,E.S., Hart,E.A.,
            Heath,P.D., Henderson,C.D., Hopkins,B.L., Howard,P.J., Howden,P.J.,
            Huckle,E., Johnson,C., Johnson,D., Joy,A.A., Kay,M., Keenan,S.,
            Kershaw,J.K., Kimberley,A.M., King,A., Knights,A., Laird,G.K.,
            Langford,C., Lawlor,S., Leongamornlert,D.A., Leversha,M., Lloyd,C.,
            Lloyd,D.M., Lovell,J., Martin,S., Mashreghi-Mohammadi,M.,
            Matthews,L., McLaren,S., McLay,K.E., McMurray,A., Milne,S.,
            Nickerson,T., Nisbett,J., Nordsiek,G., Pearce,A.V., Peck,A.I.,
            Porter,K.M., Pandian,R., Pelan,S., Phillimore,B., Povey,S.,
            Ramsey,Y., Rand,V., Scharfe,M., Sehra,H.K., Shownkeen,R.,
            Sims,S.K., Skuce,C.D., Smith,M., Steward,C.A., Swarbreck,D.,
            Sycamore,N., Tester,J., Thorpe,A., Tracey,A., Tromans,A.,
            Thomas,D.W., Wall,M., Wallis,J.M., West,A.P., Whitehead,S.L.,
            Willey,D.L., Williams,S.A., Wilming,L., Wray,P.W., Young,L.,
            Ashurst,J.L., Coulson,A., Blocker,H., Durbin,R., Sulston,J.E.,
            Hubbard,T., Jackson,M.J., Bentley,D.R., Beck,S., Rogers,J. and
            Dunham,I.
  TITLE     DNA sequence and analysis of human chromosome 9
  JOURNAL   Nature 429 (6990), 369-374 (2004)
   PUBMED   15164053
REFERENCE   10 (bases 1 to 4268)
  AUTHORS   Verbsky,J.W., Wilson,M.P., Kisseleva,M.V., Majerus,P.W. and
            Wente,S.R.
  TITLE     The synthesis of inositol hexakisphosphate. Characterization of
            human inositol 1,3,4,5,6-pentakisphosphate 2-kinase
  JOURNAL   J Biol Chem 277 (35), 31857-31862 (2002)
   PUBMED   12084730
  REMARK    GeneRIF: identified a genomic DNA sequence on chromosome 9 that
            encodes inositol 1,3,4,5,6-pentakisphosphate 2-kinase;
            characterization of the enzyme
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC026154.2 and AL157827.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_022755.5.
            
            Summary: The protein encoded by this gene is a kinase that
            phosphorylates position 2 of inositol-1,3,4,5,6-pentakisphosphate
            to form inositol-1,2,3,4,5,6-hexakisphosphate (InsP6). InsP6 has a
            variety of functions, including stimulation of DNA repair,
            endocytosis, and mRNA export. [provided by RefSeq, Nov 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF351202.1, BC026154.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000287996.8/ ENSP00000287996.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2890              BC026154.2         137-3026
            2891-4268           AL157827.17        133394-134771       c
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q22.31"
     gene            1..4268
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /note="inositol-pentakisphosphate 2-kinase"
                     /db_xref="GeneID:64768"
                     /db_xref="HGNC:HGNC:14645"
                     /db_xref="MIM:619043"
     exon            1..224
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..1619
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /EC_number="2.7.1.158"
                     /note="inositol 1,3,4,5,6-pentakisphosphate 2-kinase; IPK1
                     homolog; insP5 2-kinase; ins(1,3,4,5,6)P5 2-kinase"
                     /codon_start=1
                     /product="inositol-pentakisphosphate 2-kinase"
                     /protein_id="NP_073592.1"
                     /db_xref="CCDS:CCDS6699.1"
                     /db_xref="GeneID:64768"
                     /db_xref="HGNC:HGNC:14645"
                     /db_xref="MIM:619043"
                     /translation="MEEGKMDENEWGYHGEGNKSLVVAHAQRCVVLRFLKFPPNRKKT
                     SEEIFQHLQNIVDFGKNVMKEFLGENYVHYGEVVQLPLEFVKQLCLKIQSERPESRCD
                     KDLDTLSGYAMCLPNLTRLQTYRFAEHRPILCVEIKPKCGFIPFSSDVTHEMKHKVCR
                     YCMHQHLKVATGKWKQISKYCPLDLYSGNKQRMHFALKSLLQEAQNNLKIFKNGELIY
                     GCKDARSPVADWSELAHHLKPFFFPSNGLASGPHCTRAVIRELVHVITRVLLSGSDKG
                     RAGTLSPGLGPQGPRVCEASPFSRSLRCQGKNTPERSGLPKGCLLYKTLQVQMLDLLD
                     IEGLYPLYNRVERYLEEFPEERKTLQIDGPYDEAFYQKLLDLSTEDDGTVAFALTKVQ
                     QYRVAMTAKDCSIMIALSPCLQDASSDQRPVVPSSRSRFAFSVSVLDLDLKPYESIPH
                     QYKLDGKIVNYYSKTVRAKDNAVMSTRFKESEDCTLVLHKV"
     exon            225..272
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            273..368
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            369..435
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            436..557
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            558..647
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            648..706
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            707..779
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            780..1059
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1210
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1313
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1393
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..4268
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2871..2876
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /note="hexamer: ATTATA"
     polyA_site      2895
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /note="major polyA site"
     polyA_site      2980
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
     regulatory      4243..4248
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
                     /note="hexamer: AATAAA"
     polyA_site      4268
                     /gene="IPPK"
                     /gene_synonym="bA476B13.1; C9orf12; INSP5K2; IP5K; IPK1"
ORIGIN      
        1 agtcgccgcg gcggggtcga gcgggggcgg cagctgacag gcgctcctcc cccgaccgca
       61 gcggctggtt cccagcgagg cgggcggccc ccagctcgcg tccccgagtc ctagcccgcg
      121 aggcgccagg gctgcgcctg ggcatggaag aggggaagat ggacgagaat gaatgggggt
      181 accacggaga gggcaataag agcctggtgg tggcccacgc gcagcgctgc gtcgtgctgc
      241 ggtttctgaa gtttcctcca aataggaaga agacctcgga agagatattt caacacctgc
      301 agaacatagt ggactttggg aaaaatgtca tgaaggagtt tttgggggag aactatgttc
      361 attatgggga ggtcgttcag ctacctttag agtttgtgaa acagctttgt ttaaagatac
      421 aatctgaaag accagagtct cgctgtgaca aggacctgga tactctcagt ggttacgcta
      481 tgtgccttcc taatttaacc agactccaaa cctaccgctt tgcagagcac cggccgattc
      541 tgtgtgtaga gattaagcca aaatgtgggt ttattccttt ctcgagtgat gtcacgcatg
      601 agatgaagca taaggtctgt cgatactgca tgcaccagca cctcaaggta gcaactggga
      661 agtggaagca gatcagcaaa tactgtcccc ttgatctcta ctcaggaaac aaacagagaa
      721 tgcactttgc cttgaagagt ttgctgcagg aggcacagaa caacttgaag atatttaaga
      781 atggtgagct gatttacggc tgcaaagatg cccggagccc cgtggctgac tggagcgagc
      841 ttgcacacca cctgaagccg ttcttcttcc cttccaacgg cctggccagt gggccccact
      901 gcacaagggc tgtgatcagg gagctggtgc acgtgatcac acgggtgctg ctgagtggct
      961 cggacaaggg ccgggcaggc accctgagtc cggggctcgg gcctcagggc ccgcgagtct
     1021 gcgaagccag ccctttcagt aggagccttc gctgccaagg aaaaaacacc ccagagcgct
     1081 cggggttacc gaagggctgt cttctgtaca aaaccctcca ggtgcagatg ttggacctgc
     1141 tggacatcga aggcctctac cctctgtaca accgggttga gcgatacctg gaagagtttc
     1201 ccgaggagag aaaaacctta caaatagatg ggccttatga tgaagcattt taccagaagc
     1261 tgcttgacct ttccactgag gatgacggga cagtggcctt cgcgctaacg aaggtgcagc
     1321 agtaccgcgt cgccatgact gccaaggact gctccatcat gattgcactg tctccctgtc
     1381 tgcaggatgc cagctctgat caaaggcctg tcgtcccttc atcgaggtcc aggtttgcct
     1441 tttccgtgtc tgtgctggac cttgacctca agccctacga gagcattccc catcagtata
     1501 aactggacgg caagatcgtc aactattatt caaagactgt acgtgccaaa gacaacgccg
     1561 tgatgtcgac tcggttcaag gaaagcgaag attgcacatt agttctccac aaggtctaac
     1621 tctttccctg cagtgtcttt gaaacttgaa cataatgtga aggctgaatg atagagatat
     1681 tttctgttgt gttgggtgac ctttggttgt gaatgttttt gcttttaacc ccttttgagg
     1741 tgggattgcc tcttggagac atggaattga agagcactag aaacaacttc ctggacaagg
     1801 aatgtaggaa gtgagtgctg tgtcccagga agctgctcac actcttaaaa tggaagtgtc
     1861 cgttaagccc tgggaagacg ttctggatag ttcttctttc ccaaccaggg ctcatgtctg
     1921 attctctaat gcgaaaagcc ttattctaag acccaaggtt tggatctgct accaccagac
     1981 tcctaacata gaaaacttga attgtcacat acattttaca gtttggactt ttaagaaaac
     2041 atggatacta ctggaacttc ccccagctga gttacatggt cactttttca gtgcaagcca
     2101 catatcaaca caggttttta ggtggttccc tgctgcacag cgtgacctcg tggtcgcgca
     2161 gatgccgatt ctgagcagtg tagactcagg gtccctgtgg aggtgctgaa gcccacagtt
     2221 ccacacaggg cagcagggag cgcaaggctc aggtcaggat ggacaccact catgcgagca
     2281 ttgacctttt tttttttgct ttacaattta atctttcaca tgagggggag atgagctgtt
     2341 ctttcccact aaaaattgct gctgtagaag ttggaggtgg agctgtgact ggctgagctg
     2401 ctgtggccgg gctgggcagt gtgccccaac agctcagtgc tttcctgaca ctccagtgtc
     2461 tggggtggtt gaggagcgag ttctctcttc ctcccagacc aagttcctcc ctcgggtttg
     2521 ccttgagacg tgttgcgttt ttgggccccg tggcctctcc ctgttaggct gccacaggcc
     2581 ctgcttctgg aaggtgaaca gctcctggct gctgccgaga gggttctcgt tggggtcacc
     2641 aaagtgtgcc cggctgctat gaaaaacgtt gggaatcttg gtttcagttt tttattctat
     2701 gctaggttgt acagacttat ttatatcatc gttttgaggg actaatggag gcttattgta
     2761 acatataata ttagtgaaac catggaatta tatgaaaatg atacatgaga aataaggaaa
     2821 ctattttgct gattgtaaat ttttgtggga aattttgtga taacttgaga attatacttg
     2881 tttgaatcaa ggcaactctt ctagaattta ttgttcaatt ctgtcatatt tacttctaat
     2941 tctactctca gaggtgcaaa aagatactta tctaactgga cacttttact gttttcttct
     3001 cacttccaat gacaccgtct catacacacg ggtcctgtgt gctgggatgg gggctttggt
     3061 gaagtgcacg gagctcacgg acaagccgca ggctctggga agcgtggagg tagtggccgt
     3121 tgagtactgt cttgacccaa gggagttcac tgttgctggg ctggggctgg ggcagcctgg
     3181 tttgggatga ttttgcccat tgtcacggtg tgtggaggat gctgggtcct gggttcaagt
     3241 gcacatgtga gggaacccca gtccgtggga aggtgggctg ggctccctag gtccttcagg
     3301 gctgcaagag ttattgccta gcacagggcc cagacagggc tggactagac acgatgaaaa
     3361 cagcctgtat ttgagggaga agagaaaaca gatggagacc cccactctgt ctggtcttgg
     3421 ctcccaccag cccgggctgt gaggagcccc tgagccgcgg gtgccactgc tgtggatgcg
     3481 tttctggccc gtcttggaaa ctgctgctgc tgcgtagtca tgacggccct gactggtttg
     3541 agacaacacg cgttcacaga tcttaacaat gctaaatggg cgactgatga ggtcacggtt
     3601 cttaaggcac atccgggacg atcgatatag cacttaggct atgcaggggg gtctttcttt
     3661 cataaaggac ctcattcctt gcgtcccagc ccaggaacag tgctgtcttt gctgtcttag
     3721 gccattcttg ttattaagct gacagcttca ctttcttgtg gaagaccaag aacagcatga
     3781 ggacttgctg agactgcctc acggtgccac catgcggacc ggggggaggc agggagtgag
     3841 ggtgtggctc ccctgggcta ctaaggacag tttaaagatg aggcgccata ggcatccgtc
     3901 tccagagccc ccaggaggca ggaaccagga taagggtggc ttctcccagc tgagtaatgt
     3961 ggccaccatg tccaaggtgg actcggtgtg aggctgcagt gggtggggga tggatcctcc
     4021 ccacacatct gaggacacac aaccacttgg tacattttct cagatgttaa gcttattttt
     4081 ataaaccaca ttgtacgtgt cacttttatt tggcatttta tacttaacta gttgtattta
     4141 tttttcatgt catgagagtt tccatataat gtgccataac tgcatagaca gcacgtcttc
     4201 agggtgtggt ttctaagcag aaaatagcaa atgtttatat tgaataaaat tgcatgttcc
     4261 tccacgca
//
