LOCUS       NM_033547               3146 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens integrator complex subunit 4 (INTS4), mRNA.
ACCESSION   NM_033547 XM_495889
VERSION     NM_033547.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Lin,M.H., Jensen,M.K., Elrod,N.D., Chu,H.F., Haseley,M., Beam,A.C.,
            Huang,K.L., Chiang,W., Russell,W.K., Williams,K., Proschel,C.,
            Wagner,E.J. and Tong,L.
  TITLE     Cytoplasmic binding partners of the Integrator endonuclease INTS11
            and its paralog CPSF73 are required for their nuclear function
  JOURNAL   Mol Cell 84 (15), 2900-2917 (2024)
   PUBMED   39032490
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3146)
  AUTHORS   Pfleiderer,M.M. and Galej,W.P.
  TITLE     Structure of the catalytic core of the Integrator complex
  JOURNAL   Mol Cell 81 (6), 1246-1259 (2021)
   PUBMED   33548203
REFERENCE   4  (bases 1 to 3146)
  AUTHORS   Beckedorff,F., Blumenthal,E., daSilva,L.F., Aoi,Y., Cingaram,P.R.,
            Yue,J., Zhang,A., Dokaneheifard,S., Valencia,M.G., Gaidosh,G.,
            Shilatifard,A. and Shiekhattar,R.
  TITLE     The Human Integrator Complex Facilitates Transcriptional Elongation
            by Endonucleolytic Cleavage of Nascent Transcripts
  JOURNAL   Cell Rep 32 (3), 107917 (2020)
   PUBMED   32697989
REFERENCE   5  (bases 1 to 3146)
  AUTHORS   Sabath,K., Staubli,M.L., Marti,S., Leitner,A., Moes,M. and Jonas,S.
  TITLE     INTS10-INTS13-INTS14 form a functional module of Integrator that
            binds nucleic acids and the cleavage module
  JOURNAL   Nat Commun 11 (1), 3422 (2020)
   PUBMED   32647223
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3146)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   7  (bases 1 to 3146)
  AUTHORS   Jodoin,J.N., Sitaram,P., Albrecht,T.R., May,S.B., Shboul,M.,
            Lee,E., Reversade,B., Wagner,E.J. and Lee,L.A.
  TITLE     Nuclear-localized Asunder regulates cytoplasmic dynein localization
            via its role in the integrator complex
  JOURNAL   Mol Biol Cell 24 (18), 2954-2965 (2013)
   PUBMED   23904267
REFERENCE   8  (bases 1 to 3146)
  AUTHORS   Takata,H., Nishijima,H., Maeshima,K. and Shibahara,K.
  TITLE     The integrator complex is required for integrity of Cajal bodies
  JOURNAL   J Cell Sci 125 (Pt 1), 166-175 (2012)
   PUBMED   22250197
  REMARK    GeneRIF: findings demonstrate that the integrator complex is
            essential for snRNA maturation and Cajal body homeostasis
REFERENCE   9  (bases 1 to 3146)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   10 (bases 1 to 3146)
  AUTHORS   Baillat,D., Hakimi,M.A., Naar,A.M., Shilatifard,A., Cooch,N. and
            Shiekhattar,R.
  TITLE     Integrator, a multiprotein mediator of small nuclear RNA
            processing, associates with the C-terminal repeat of RNA polymerase
            II
  JOURNAL   Cell 123 (2), 265-276 (2005)
   PUBMED   16239144
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC015664.2, BC009995.2 and BC009859.2.
            
            On Nov 22, 2018 this sequence version replaced NM_033547.3.
            
            Summary: INTS4 is a subunit of the Integrator complex, which
            associates with the C-terminal domain of RNA polymerase II large
            subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of
            small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690)
            (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BK005723.1, SRR1803611.49607.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000534064.6/ ENSP00000434466.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-389               BC015664.2         4-392
            390-1396            BC009995.2         388-1394
            1397-3146           BC009859.2         59-1808
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q14.1"
     gene            1..3146
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /note="integrator complex subunit 4"
                     /db_xref="GeneID:92105"
                     /db_xref="HGNC:HGNC:25048"
                     /db_xref="MIM:611348"
     exon            1..79
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..2917
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /note="MSTP093"
                     /codon_start=1
                     /product="integrator complex subunit 4"
                     /protein_id="NP_291025.3"
                     /db_xref="CCDS:CCDS31644.1"
                     /db_xref="GeneID:92105"
                     /db_xref="HGNC:HGNC:25048"
                     /db_xref="MIM:611348"
                     /translation="MAAHLKKRVYEEFTKVVQPQEEIATKKLRLTKPSKSAALHIDLC
                     KATSPADALQYLLQFARKPVEAESVEGVVRILLEHYYKENDPSVRLKIASLLGLLSKT
                     AGFSPDCIMDDAINILQNEKSHQVLAQLLDTLLAIGTKLPENQAIQMRLVDVACKHLT
                     DTSHGVRNKCLQLLGNLGSLEKSVTKDAEGLAARDVQKIIGDYFSDQDPRVRTAAIKA
                     MLQLHERGLKLHQTIYNQACKLLSDDYEQVRSAAVQLIWVVSQLYPESIVPIPSSNEE
                     IRLVDDAFGKICHMVSDGSWVVRVQAAKLLGSMEQVSSHFLEQTLDKKLMSDLRRKRT
                     AHERAKELYSSGEFSSGRKWGDDAPKEEVDTGAVNLIESGACGAFVHGLEDEMYEVRI
                     AAVEALCMLAQSSPSFAEKCLDFLVDMFNDEIEEVRLQSIHTMRKISNNITLREDQLD
                     TVLAVLEDSSRDIREALHELLCCTNVSTKEGIHLALVELLKNLTKYPTDRDSIWKCLK
                     FLGSRHPTLVLPLVPELLSTHPFFDTAEPDMDDPAYIAVLVLIFNAAKTCPTMPALFS
                     DHTFRHYAYLRDSLSHLVPALRLPGRKLVSSAVSPSIIPQEDPSQQFLQQSLERVYSL
                     QHLDPQGAQELLEFTIRDLQRLGELQSELAGVADFSATYLRCQLLLIKALQEKLWNVA
                     APLYLKQSDLASAAAKQIMEETYKMEFMYSGVENKQVVIIHHMRLQAKALQLIVTART
                     TRGLDPLFGMCEKFLQEVDFFQRYFIADLPHLQDSFVDKLLDLMPRLMTSKPAEVVKI
                     LQTMLRQSAFLHLPLPEQIHKASATIIEPAGESDNPLRFTSGLVVALDVDATLEHVQD
                     PQNTVKVQVLYPDGQAQMIHPKPADFRNPGPGRHRLITQVYLSHTAWTEACQVEVRLL
                     LAYNSSARIPKCPWMEGGEMSPQVETSIEGTIPFSKPVKVYIMPKPARR"
     exon            80..271
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            272..389
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            390..496
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            497..682
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            683..733
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            734..822
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            823..943
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1015
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1190
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1396
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1539
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1655
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1789
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1947
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2041
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2122
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2253
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..2313
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            2314..2473
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            2474..2617
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            2618..2738
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     exon            2739..3146
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3125..3130
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /note="hexamer: AATAAA"
     polyA_site      3146
                     /gene="INTS4"
                     /gene_synonym="INT4; MST093"
                     /note="major polyA site"
ORIGIN      
        1 aagctgagag ggcccgcggg taggcatggc ggcgcacctt aagaagcggg tttatgagga
       61 attcacgaaa gtggttcagc cacaggagga aattgctact aagaaactcc gactaacaaa
      121 accaagtaaa tctgcagcac tccacataga tctgtgtaaa gctacctccc cagcagatgc
      181 tttgcaatac ttgctccagt ttgccaggaa gcctgtcgag gcggaaagcg tagagggagt
      241 agtcaggatt ctcttggaac attattacaa ggagaatgat ccatctgtga gactgaaaat
      301 tgcatcattg ttgggtttat tatcaaagac agcaggattt tcaccagact gcattatgga
      361 tgatgccatc aacatcctgc agaatgaaaa gtctcatcaa gtcctagctc aactgctgga
      421 tactttgctt gcaattggca ctaagctacc agagaatcaa gctatccaaa tgcgattagt
      481 tgatgtggcc tgcaagcatc tgacagatac gtctcatggt gtaagaaata agtgcctgca
      541 gttacttggc aatcttggct ctttggagaa aagtgtcaca aaagatgcag aaggcctagc
      601 tgccagagat gtccagaaga ttatagggga ttacttcagt gaccaagacc cacgtgtcag
      661 aacagcagct ataaaagcca tgttgcagct ccatgaaaga ggactgaaat tacaccaaac
      721 aatttataat caggcctgta aattactctc tgatgactat gaacaagtgc gcagtgctgc
      781 agtccagctt atctgggtcg tcagtcagct ctatcctgaa agcattgtcc caattccttc
      841 ttctaatgaa gaaatacgct tagttgatga tgcgtttggc aaaatttgtc acatggtcag
      901 tgatggctct tgggtggttc gtgttcaggc agcaaaactg ttgggctcta tggagcaagt
      961 cagttctcat ttcttggagc agacccttga caagaagctg atgtcagatc tgaggaggaa
     1021 acgtactgca catgagcgtg ccaaggaact ttacagttcg ggggagtttt ccagtggcag
     1081 aaagtgggga gatgatgctc ccaaggaaga agtagatacc ggggctgtga acttgattga
     1141 gtcaggagct tgtggagctt ttgttcatgg gttggaagat gagatgtatg aggttcgtat
     1201 tgctgctgtg gaggccctct gcatgttggc ccagtcttca ccctcttttg ctgagaagtg
     1261 ccttgatttc ctagttgaca tgttcaacga tgaaattgag gaagtacgtc tgcagtctat
     1321 acataccatg agaaaaatct ctaacaacat caccctccga gaagatcagc ttgacactgt
     1381 cctggctgtg ctagaggatt catccagaga tattcgagag gctcttcatg aactcttatg
     1441 ctgtactaat gtttcaacca aagaagggat tcatcttgca ttggtggagc tgctgaaaaa
     1501 tttaaccaag taccctactg atagggactc catatggaag tgcttgaagt ttctgggaag
     1561 tcggcatcca accctggtgc ttcccttggt gccagagctt ctgagcaccc acccattttt
     1621 tgacacagct gaaccagaca tggatgatcc agcttatatt gcagttttgg tacttatttt
     1681 caatgctgct aaaacctgtc caacaatgcc agcattgttc tcagatcaca ccttcaggca
     1741 ctatgcctac ctccgagaca gtctttctca tcttgttcct gccttgaggt taccaggtag
     1801 aaaactggtg tcatcagctg tttctcccag catcatacct caagaggatc cttcccagca
     1861 gttcctgcag cagagccttg aaagagtgta tagtcttcag cacttggacc ctcagggagc
     1921 ccaggagctg ctggaattca ccatcaggga tctgcaaaga cttggagaac ttcaatctga
     1981 attggcagga gtagctgatt tctctgccac ctatcttcgc tgtcaactac ttctcatcaa
     2041 ggccttgcag gaaaagttgt ggaatgtagc tgcccctttg tatttgaagc agagtgattt
     2101 ggcctcagca gcagcgaaac agattatgga agagacctac aaaatggaat tcatgtacag
     2161 tggtgtggag aataagcagg tggtgattat acatcacatg aggctgcagg ccaaagcttt
     2221 gcaacttata gtaacagcac gaactacacg aggacttgac cccttatttg ggatgtgtga
     2281 aaaattttta caggaagtag acttttttca gaggtatttc atcgctgatt tgccccactt
     2341 gcaggacagc tttgtggaca aactccttga ccttatgccc cgactcatga catccaaacc
     2401 tgcagaagtg gtcaaaattc tacagaccat gctgcgacag agtgcctttc tgcatctccc
     2461 gcttccagag cagatccaca aagcctcagc caccatcatc gagccagcgg gcgagtcaga
     2521 caaccctttg cggtttacct ctgggttggt ggttgccctg gatgttgatg caaccctgga
     2581 gcatgtgcag gatcctcaga acactgttaa ggtccaggtc ttatatccag atggccaggc
     2641 tcagatgatt caccccaagc ctgcagactt ccggaatcct ggcccagggc ggcaccggct
     2701 catcactcag gtttatctct cccacaccgc ttggacagag gcatgccagg tggaagtgag
     2761 gctgctgctg gcctacaact ccagtgctcg cattccaaaa tgcccctgga tggagggtgg
     2821 tgagatgtca ccacaggtgg aaaccagcat cgagggcacc attcccttca gcaagcctgt
     2881 aaaagtttat ataatgccca aacctgcacg gcgctaaggc aaaaacagtc ttcccaaccg
     2941 tggcctagag ggcccttctt aggtgtcaga atgagccaag cctgaagcac ttcacctgga
     3001 attgatgtgt aggcttaagg agtatgtgac ccttacagtc tcatctggta tcaaacacag
     3061 gataaattgt ttcttcatta aaaaataaaa aaccttcaag tctacttacc cttctcctgt
     3121 ccacaataaa gttgagaaaa caccaa
//
