LOCUS       NM_001080453            6981 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens integrator complex subunit 1 (INTS1), mRNA.
ACCESSION   NM_001080453 NM_015674 XM_291222
VERSION     NM_001080453.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Lin,M.H., Jensen,M.K., Elrod,N.D., Chu,H.F., Haseley,M., Beam,A.C.,
            Huang,K.L., Chiang,W., Russell,W.K., Williams,K., Proschel,C.,
            Wagner,E.J. and Tong,L.
  TITLE     Cytoplasmic binding partners of the Integrator endonuclease INTS11
            and its paralog CPSF73 are required for their nuclear function
  JOURNAL   Mol Cell 84 (15), 2900-2917 (2024)
   PUBMED   39032490
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Confino,S., Wexler,Y., Medvetzky,A., Elazary,Y., Ben-Moshe,Z.,
            Reiter,J., Dor,T., Edvardson,S., Prag,G., Harel,T. and Gothilf,Y.
  TITLE     A deleterious variant of INTS1 leads to disrupted sleep-wake cycles
  JOURNAL   Dis Model Mech 17 (8) (2024)
   PUBMED   39189071
  REMARK    GeneRIF: A deleterious variant of INTS1 leads to disrupted
            sleep-wake cycles.
REFERENCE   3  (bases 1 to 6981)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 6981)
  AUTHORS   Pfleiderer,M.M. and Galej,W.P.
  TITLE     Structure of the catalytic core of the Integrator complex
  JOURNAL   Mol Cell 81 (6), 1246-1259 (2021)
   PUBMED   33548203
REFERENCE   5  (bases 1 to 6981)
  AUTHORS   Beckedorff,F., Blumenthal,E., daSilva,L.F., Aoi,Y., Cingaram,P.R.,
            Yue,J., Zhang,A., Dokaneheifard,S., Valencia,M.G., Gaidosh,G.,
            Shilatifard,A. and Shiekhattar,R.
  TITLE     The Human Integrator Complex Facilitates Transcriptional Elongation
            by Endonucleolytic Cleavage of Nascent Transcripts
  JOURNAL   Cell Rep 32 (3), 107917 (2020)
   PUBMED   32697989
REFERENCE   6  (bases 1 to 6981)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   7  (bases 1 to 6981)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   8  (bases 1 to 6981)
  AUTHORS   Nusbaum,C., Mikkelsen,T.S., Zody,M.C., Asakawa,S., Taudien,S.,
            Garber,M., Kodira,C.D., Schueler,M.G., Shimizu,A., Whittaker,C.A.,
            Chang,J.L., Cuomo,C.A., Dewar,K., FitzGerald,M.G., Yang,X.,
            Allen,N.R., Anderson,S., Asakawa,T., Blechschmidt,K., Bloom,T.,
            Borowsky,M.L., Butler,J., Cook,A., Corum,B., DeArellano,K.,
            DeCaprio,D., Dooley,K.T., Dorris,L. 3rd, Engels,R., Glockner,G.,
            Hafez,N., Hagopian,D.S., Hall,J.L., Ishikawa,S.K., Jaffe,D.B.,
            Kamat,A., Kudoh,J., Lehmann,R., Lokitsang,T., Macdonald,P.,
            Major,J.E., Matthews,C.D., Mauceli,E., Menzel,U., Mihalev,A.H.,
            Minoshima,S., Murayama,Y., Naylor,J.W., Nicol,R., Nguyen,C.,
            O'Leary,S.B., O'Neill,K., Parker,S.C., Polley,A., Raymond,C.K.,
            Reichwald,K., Rodriguez,J., Sasaki,T., Schilhabel,M., Siddiqui,R.,
            Smith,C.L., Sneddon,T.P., Talamas,J.A., Tenzin,P., Topham,K.,
            Venkataraman,V., Wen,G., Yamazaki,S., Young,S.K., Zeng,Q.,
            Zimmer,A.R., Rosenthal,A., Birren,B.W., Platzer,M., Shimizu,N. and
            Lander,E.S.
  TITLE     DNA sequence and analysis of human chromosome 8
  JOURNAL   Nature 439 (7074), 331-335 (2006)
   PUBMED   16421571
REFERENCE   9  (bases 1 to 6981)
  AUTHORS   Baillat,D., Hakimi,M.A., Naar,A.M., Shilatifard,A., Cooch,N. and
            Shiekhattar,R.
  TITLE     Integrator, a multiprotein mediator of small nuclear RNA
            processing, associates with the C-terminal repeat of RNA polymerase
            II
  JOURNAL   Cell 123 (2), 265-276 (2005)
   PUBMED   16239144
REFERENCE   10 (bases 1 to 6981)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC093734.3, DB113520.1, AI935093.1, BM553299.1, BM547069.1,
            BF346658.1, BX324321.2, AB037861.1 and BC069262.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001080453.2.
            
            Summary: INTS1 is a subunit of the Integrator complex, which
            associates with the C-terminal domain of RNA polymerase II large
            subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of
            small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690)
            (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000404767.8/ ENSP00000385722.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 AC093734.3         10803-10809         c
            8-569               DB113520.1         1-562
            570-844             AI935093.1         284-558
            845-1363            BM553299.1         267-785
            1364-1784           BM547069.1         367-787
            1785-2173           BF346658.1         313-701
            2174-2555           BX324321.2         648-1029
            2556-5692           AB037861.1         9-3145
            5693-6981           BC069262.1         1716-3004
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.3"
     gene            1..6981
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /note="integrator complex subunit 1"
                     /db_xref="GeneID:26173"
                     /db_xref="HGNC:HGNC:24555"
                     /db_xref="MIM:611345"
     exon            1..67
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            68..166
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..6681
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /codon_start=1
                     /product="integrator complex subunit 1"
                     /protein_id="NP_001073922.2"
                     /db_xref="CCDS:CCDS47526.1"
                     /db_xref="GeneID:26173"
                     /db_xref="HGNC:HGNC:24555"
                     /db_xref="MIM:611345"
                     /translation="MNRAKPTTVRRPSAAAKPSGHPPPGDFIALGSKGQANESKTAST
                     LLKPAPSGLPSERKRDAAAALSSASALTGLTKRPKLSSTPPLSALGRLAEAAVAEKRA
                     ISPSIKEPSVVPIEVLPTVLLDEIEAAELEGNDDRIEGVLCGAVKQLKVTRAKPDSTL
                     YLSLMYLAKIKPNIFATEGVIEALCSLLRRDASINFKAKGNSLVSVLACNLLMAAYEE
                     DENWPEIFVKVYIEDSLGERIWVDSPHCKTFVDNIQTAFNTRMPPRSVLLQGEAGRVA
                     GDLGAGSSPHPSLTEEEDSQTELLIAEEKLSPEQEGQLMPRYEELAESVEEYVLDMLR
                     DQLNRRQPIDNVSRNLLRLLTSTCGYKEVRLLAVQKLEMWLQNPKLTRPAQDLLMSVC
                     MNCNTHGSEDMDVISHLIKIRLKPKVLLNHFMLCIRELLSAHKDNLGTTIKLVIFNEL
                     SSARNPNNMQVLYTALQHSSELAPKFLAMVFQDLLTNKDDYLRASRALLREIIKQTKH
                     EINFQAFCLGLMQERKEPQYLEMEFKERFVVHITDVLAVSMMLGITAQVKEAGIAWDK
                     GEKRNLEVLRSFQNQIAAIQRDAVWWLHTVVPSISKLAPKDYVHCLHKVLFTEQPETY
                     YKWDNWPPESDRNFFLRLCSEVPILEDTLMRILVIGLSRELPLGPADAMELADHLVKR
                     AAAVQADDVEVLKVGRTQLIDAVLNLCTYHHPENIQLPPGYQPPNLAISTLYWKAWPL
                     LLVVAAFNPENIGLAAWEEYPTLKMLMEMVMTNNYSYPPCTLTDEETRTEMLNRELQT
                     AQREKQEILAFEGHLAAASTKQTITESSSLLLSQLTSLDPQGPPRRPPPHILDQVKSL
                     NQSLRLGHLLCRSRNPDFLLHIIQRQASSQSMPWLADLVQSSEGSLDVLPVQCLCEFL
                     LHDAVDDAASGEEDDEGESKEQKAKKRQRQQKQRQLLGRLQDLLLGPKADEQTTCEVL
                     DYFLRRLGSSQVASRVLAMKGLSLVLSEGSLRDGEEKEPPMEEDVGDTDVLQGYQWLL
                     RDLPRLPLFDSVRSTTALALQQAIHMETDPQTISAYLIYLSQHTPVEEQAQHSDLALD
                     VARLVVERSTIMSHLFSKLSPSAASDAVLSALLSIFSRYVRRMRQSKEGEEVYSWSES
                     QDQVFLRWSSGETATMHILVVHAMVILLTLGPPRADDSEFQALLDIWFPEEKPLPTAF
                     LVDTSEEALLLPDWLKLRMIRSEVLRLVDAALQDLEPQQLLLFVQSFGIPVSSMSKLL
                     QFLDQAVAHDPQTLEQNIMDKNYMAHLVEVQHERGASGGQTFHSLLTASLPPRRDSTE
                     APKPKSSPEQPIGQGRIRVGTQLRVLGPEDDLAGMFLQIFPLSPDPRWQSSSPRPVAL
                     ALQQALGQELARVVQGSPEVPGITVRVLQALATLLSSPHGGALVMSMHRSHFLACPLL
                     RQLCQYQRCVPQDTGFSSLFLKVLLQMLQWLDSPGVEGGPLRAQLRMLASQASAGRRL
                     SDVRGGLLRLAEALAFRQDLEVVSSTVRAVIATLRSGEQCSVEPDLISKVLQGLIEVR
                     SPHLEELLTAFFSATADAASPFPACKPVVVVSSLLLQEEEPLAGGKPGADGGSLEAVR
                     LGPSSGLLVDWLEMLDPEVVSSCPDLQLRLLFSRRKGKGQAQVPSFRPYLLTLFTHQS
                     SWPTLHQCIRVLLGKSREQRFDPSASLDFLWACIHVPRIWQGRDQRTPQKRREELVLR
                     VQGPELISLVELILAEAETRSQDGDTAACSLIQARLPLLLSCCCGDDESVRKVTEHLS
                     GCIQQWGDSVLGRRCRDLLLQLYLQRPELRVPVPEVLLHSEGAASSSVCKLDGLIHRF
                     ITLLADTSDSRALENRGADASMACRKLAVAHPLLLLRHLPMIAALLHGRTHLNFQEFR
                     QQNHLSCFLHVLGLLELLQPHVFRSEHQGALWDCLLSFIRLLLNYRKSSRHLAAFINK
                     FVQFIHKYITYNAPAAISFLQKHADPLHDLSFDNSDLVMLKSLLAGLSLPSRDDRTDR
                     GLDEEGEEESSAGSLPLVSVSLFTPLTAAEMAPYMKRLSRGQTVEDLLEVLSDIDEMS
                     RRRPEILSFFSTNLQRLMSSAEECCRNLAFSLALRSMQNSPSIAAAFLPTFMYCLGSQ
                     DFEVVQTALRNLPEYALLCQEHAAVLLHRAFLVGMYGQMDPSAQISEALRILHMEAVM
                     "
     exon            167..457
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            458..654
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            655..792
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            793..952
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1058
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1245
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1391
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1533
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            1534..1710
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1819
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..1940
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            1941..2018
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2176
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2273
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            2274..2365
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            2366..2426
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            2427..2624
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            2625..2754
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            2755..2934
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            2935..3084
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            3085..3264
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            3265..3369
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            3370..3537
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            3538..3649
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            3650..3811
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            3812..3958
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            3959..4057
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            4058..4182
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            4183..4437
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            4438..4597
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            4598..4738
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            4739..4922
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            4923..5046
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            5047..5171
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            5172..5259
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            5260..5486
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            5487..5610
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            5611..5744
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            5745..5937
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            5938..6065
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            6066..6178
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            6179..6292
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            6293..6363
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            6364..6455
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            6456..6565
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     exon            6566..6981
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6961..6966
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /note="hexamer: AATGAA"
     polyA_site      6981
                     /gene="INTS1"
                     /gene_synonym="INT1; NDCAGF; NET28"
                     /note="major polyA site"
ORIGIN      
        1 gagatcggtt tccgattccg gggtggccgg gtggcggcgg cggcgctccg gtcgcgatgg
       61 gccagaggtg acgccgcagc cgccgggagc cgcgagggac ggggcaggat gaaccgggcc
      121 aagcccacca cggtgcgccg gcccagcgcc gcggccaaac cctcagggca ccctccccca
      181 ggagacttca ttgctctggg ctcaaagggt caggccaatg aatcgaaaac ggcgtccacc
      241 ctgctgaagc cagccccttc cggcctgcct tctgagcgca agcgggatgc ggcggccgcg
      301 ttgtccagtg cctcggccct caccggtctc accaaacgcc ccaaactctc ctccacaccc
      361 cctctgagtg ccctggggcg cctggctgag gctgcagtgg cagaaaaacg agccatttct
      421 ccgtcgatta aagagccatc tgtggtgcca attgaagtgc tgcccacggt gctgctggat
      481 gagatcgagg cggccgagct ggagggcaac gatgacagga tcgagggcgt gctgtgcggg
      541 gccgtgaagc agctgaaggt cacccgcgcc aagcctgaca gcaccctcta cctgagcctc
      601 atgtacctgg ccaagatcaa gcccaacatc ttcgccactg agggcgtcat tgaggctctg
      661 tgtagcctcc tgcggcggga cgcctccatc aacttcaagg ccaaggggaa cagcctggtg
      721 tctgtgctgg cctgtaacct cctcatggcc gcctacgagg aggacgagaa ctggcccgag
      781 atctttgtca aggtgtacat cgaggactcc ctgggggagc ggatctgggt ggacagccct
      841 cactgtaaga cgtttgtgga caacatccag acggccttca acaccagaat gccccccagg
      901 agcgtgctgc tgcaggggga ggcgggccgc gttgcgggcg acctgggtgc agggagcagc
      961 ccacacccct ccctcacgga ggaggaggac agccagacgg agttgctgat cgcggaggag
     1021 aagctgagcc ccgagcagga gggccagctc atgcccaggt acgaagagct cgcggagagc
     1081 gtggaggagt atgtcctgga catgctgcgg gaccagctga accggcgcca gcccatcgac
     1141 aacgtctcca ggaacctcct gcggctcctc acctccacct gcggctataa ggaggtgcgg
     1201 ctgctggcgg tgcagaagct ggagatgtgg ctgcagaacc ccaagctgac ccggccggcc
     1261 caggacctgc tgatgtccgt ctgcatgaac tgcaacacgc acggttccga agacatggac
     1321 gtcatctcac acctgatcaa gatccgcctc aagcccaagg tcctcctcaa ccacttcatg
     1381 ctgtgcatca gggagctgct gagcgcgcac aaggacaacc tgggcaccac catcaagttg
     1441 gtgatcttca atgagctctc cagcgcccgg aacccgaaca acatgcaggt cctctatacc
     1501 gcactgcagc acagctcaga gctggcgccc aagttcctgg ccatggtgtt ccaggacctg
     1561 ctgaccaaca aggacgacta cctgcgggcc tcgcgggccc tgctgcggga gatcatcaag
     1621 cagaccaagc acgagatcaa cttccaggcc ttctgcctgg ggctcatgca ggagcgcaag
     1681 gagccgcagt acctggagat ggagttcaag gagcgcttcg tggtgcacat caccgacgtc
     1741 ctggccgtgt ccatgatgct gggcatcaca gcgcaggtga aggaggccgg catcgcctgg
     1801 gacaaaggag aaaagaggaa cctggaagtg ctccgctcat tccagaacca gattgccgcc
     1861 atccagcggg atgccgtctg gtggctccac actgtggtcc cctccatcag caagctcgcc
     1921 cctaaggact acgtgcactg cctgcacaag gtgctgttca cagagcagcc ggagacctac
     1981 tacaagtggg acaactggcc acccgagagt gaccgcaact tcttcctgcg tctgtgctcc
     2041 gaggtgccca ttttggagga cacgctgatg cgcatcctgg tcatcgggct gtcccgggag
     2101 ctcccgctcg ggcctgcgga cgccatggag cttgctgacc acctggtgaa gcgggcggct
     2161 gccgtgcagg cggatgatgt ggaggtgctg aaggtgggga ggacccagct gatcgacgcc
     2221 gttctgaatc tgtgcaccta ccatcaccct gaaaacatcc agctcccacc ggggtaccag
     2281 cctccgaacc tcgccatctc taccctctac tggaaggcct ggcccctcct gctggtcgtc
     2341 gccgcattca acccagagaa catcggcctg gctgcgtggg aggagtaccc gaccctgaag
     2401 atgctcatgg agatggtgat gaccaacaac tactcctacc caccgtgcac cctgacggat
     2461 gaggagaccc ggacggagat gctgaaccgt gagctgcaga ccgcccagcg ggagaagcag
     2521 gagatcctgg ccttcgaggg gcacctggcg gccgcgtcca ccaagcagac catcactgag
     2581 agcagcagcc tcctcctgtc gcagctcacc agcctggacc cccaggggcc cccccggagg
     2641 cctccccctc acatcctgga tcaagtgaaa agcctcaacc agtccctccg cctcgggcac
     2701 ctcttgtgcc gcagccgaaa ccctgacttt ctcctccaca tcatccagcg gcaggcctcc
     2761 tcgcagtcca tgccctggct ggcggacctg gtacagtcca gcgagggctc cctggacgtg
     2821 ctgcccgtgc agtgtctgtg cgagttcctg ctgcacgatg ctgtggacga tgctgcttcc
     2881 ggggaggagg acgacgaggg cgagagcaag gagcagaagg ccaagaagcg gcagaggcaa
     2941 cagaagcagc ggcagctgct gggccgcctg caggacctgc tactgggccc gaaggctgat
     3001 gagcagacca cgtgtgaggt gctggactac ttcttgcggc gcctcggctc ctcccaggtg
     3061 gcctcccgcg tgctggccat gaagggtttg tcgctggtgc tttcggaggg cagcctgcgg
     3121 gacggggagg agaaggagcc ccccatggag gaggatgtgg gggacacaga tgtgctgcag
     3181 ggctatcagt ggctgctgcg ggacctgcct cgcctgcctc tgttcgacag cgtcaggagc
     3241 accacagccc tggccctgca gcaggcaatc cacatggaga ctgatcccca gaccatcagc
     3301 gcctacctga tctacttgtc ccagcacacg cctgtggagg agcaggccca gcacagcgac
     3361 ctggccctgg acgtggcccg gctggtcgtg gagcgctcca ccatcatgtc ccacctcttc
     3421 tcgaagctct ccccgagtgc cgcgtcggac gccgtgctga gcgctctgtt gtccatcttc
     3481 tcacgctacg tgaggcgcat gcggcagagc aaggagggcg aggaggtcta cagctggtcg
     3541 gagtctcagg accaggtctt cctacgctgg agcagcgggg agacagccac catgcacatc
     3601 ctcgtggtcc atgccatggt gatcctgctg acgctgggcc cgcctcgagc cgacgacagc
     3661 gagttccagg cgctgctgga catctggttt ccggaggaga agccactgcc caccgccttc
     3721 ctggtggaca catcggagga ggcgctgctg cttcctgact ggctgaagct gcgcatgatc
     3781 cgttctgagg tgctccgcct ggtggacgcc gccctgcagg acctggagcc gcagcagctg
     3841 ctgctgttcg tgcagtcgtt tggcatcccc gtgtccagca tgagcaaact cctccagttc
     3901 ctggaccagg cagtggccca cgacccccag actctggagc agaacatcat ggacaagaat
     3961 tacatggccc acctggtgga ggtccagcat gagcgcggcg cctccggagg ccagactttc
     4021 cactccttgc tcacagcctc cctgccgccc cgccgagaca gcacagaggc acccaaacca
     4081 aagagcagcc cagagcagcc cataggccag ggccggattc gggtggggac ccagctccgg
     4141 gtgctgggcc ctgaggacga cctggctggc atgttcctcc agattttccc gctcagcccg
     4201 gaccctcggt ggcagagctc cagtccccgc cccgtggccc tcgccctgca gcaggccctg
     4261 ggccaggagc tggcccgcgt cgtccagggc agccccgagg tgccgggcat cacggtgcgt
     4321 gtcctgcagg ccctcgccac cctgctcagc tccccacacg gcggtgccct ggtgatgtcc
     4381 atgcaccgta gccacttcct ggcctgcccg ctgctgcgcc agctctgcca gtaccagcgc
     4441 tgtgtgccac aggacaccgg cttctcctcg ctcttcctga aggtgctcct gcagatgctg
     4501 cagtggctgg acagccctgg cgtggagggc gggcccctgc gggcacagct caggatgctt
     4561 gccagccagg cctcagccgg gcgcaggctc agtgatgtgc gaggggggct cctgcgcctg
     4621 gccgaggccc tggccttccg tcaggacctg gaggtggtca gctccaccgt ccgtgccgtc
     4681 atcgccaccc tgaggtctgg ggagcagtgc agcgtggagc cggacctgat cagcaaagtc
     4741 ctccaggggc tgatcgaggt gaggtccccc cacctggagg agctgctgac tgcattcttc
     4801 tctgccactg cggatgctgc ctccccgttt ccagcctgta agcccgttgt ggtggtgagc
     4861 tccctgctgc tgcaggagga ggagcccctg gctgggggga agccgggtgc ggacggtggc
     4921 agcctggagg ccgtgcggct ggggccctcg tcaggcctcc tagtggactg gctggaaatg
     4981 ctggaccccg aggtggtcag cagctgcccc gacctgcagc tcaggctgct cttctcccgg
     5041 aggaagggca aaggtcaggc ccaggtgccc tcgttccgtc cctacctcct gaccctcttc
     5101 acgcatcagt ccagctggcc cacactgcac cagtgcatcc gagtcctgct gggcaagagc
     5161 cgggaacaga ggttcgaccc ctctgcctct ctggacttcc tctgggcctg catccatgtt
     5221 cctcgcatct ggcaggggcg ggaccagcgc accccgcaga agcggcggga ggagctggtg
     5281 ctgcgggtcc agggcccgga gctcatcagc ctggtggagc tgatcctggc cgaggcggag
     5341 acgcggagcc aggacgggga cacagccgcc tgcagcctca tccaggcccg gctgcccctg
     5401 ctgctcagct gctgctgtgg ggacgatgag agtgtcagga aggtgacgga gcacctgtca
     5461 ggctgcatcc agcagtgggg agacagcgtg ctgggcaggc gctgccgaga ccttctcctg
     5521 cagctctacc tacagcggcc ggagctgcgg gtgcccgtgc ctgaggtcct actgcacagc
     5581 gaaggggctg ccagcagcag cgtctgcaag ctggacggac tcatccaccg cttcatcacg
     5641 ctccttgcgg acaccagcga ctcccgggcg ttggagaacc gaggggcgga tgccagcatg
     5701 gcctgccgga agctggcggt ggcgcacccg ctgctgctgc tcaggcacct gcccatgatc
     5761 gcggcgctcc tgcacggccg cacccacctc aacttccagg agttccggca gcagaaccac
     5821 ctgagctgct tcctgcacgt gctgggcctg ctggagctgc tgcagccgca cgtgttccgc
     5881 agcgagcacc agggggcgct gtgggactgc cttctgtcct tcatccgcct gctgctgaat
     5941 tacaggaagt cctcccgcca tctggctgcc ttcatcaaca agtttgtgca gttcatccat
     6001 aagtacatta cctacaatgc cccagcagcc atctccttcc tgcagaagca cgccgacccg
     6061 ctccacgacc tgtccttcga caacagtgac ctggtgatgc tgaaatccct ccttgcaggg
     6121 ctcagcctgc ccagcaggga cgacaggacc gaccgaggcc tggacgaaga gggcgaggag
     6181 gagagctcag ccggctcctt gcccctggtc agcgtctccc tgttcacccc tctgaccgcg
     6241 gccgagatgg ccccctacat gaaacggctt tcccggggcc aaacggtgga ggatctgctg
     6301 gaggttctga gtgacataga cgagatgtcc cggcggagac ccgagatcct gagcttcttc
     6361 tcgaccaacc tgcagcggct gatgagctcg gccgaggagt gttgccgcaa cctcgccttc
     6421 agcctggccc tgcgctccat gcagaacagc cccagcattg cagccgcttt cctgcccacg
     6481 ttcatgtact gcctgggcag ccaggacttt gaggtggtgc agacggccct ccggaacctg
     6541 cctgagtacg ctctcctgtg ccaagagcac gcggctgtgc tgctccaccg ggccttcctg
     6601 gtgggcatgt acggccagat ggaccccagc gcgcagatct ccgaggccct gaggatcctg
     6661 catatggagg ccgtgatgtg agcctgtggc agccgacccc cctccaagcc ccggcccgtc
     6721 ccgtccccgg ggatcctcga ggcaaagccc aggaagcgtg ggcgttgctg gtctgtccga
     6781 ggaggtgagg gcgccgagcc ctgaggccag gcaggcccag gagcaatact ccgagccctg
     6841 gggtggctcc gggccggccg ctggcatcag gggccgtcca gcaagccctc attcaccttc
     6901 tgggccacag ccctgccgcg gagcggcgga tccccccggg catggcctgg gctggttttg
     6961 aatgaaacga cctgaactgt c
//
