LOCUS       NM_001039614            7150 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens inhibitory synaptic factor 1 (INSYN1), transcript
            variant 1, mRNA.
ACCESSION   NM_001039614 XM_005254369 XM_370873 XM_941047
VERSION     NM_001039614.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 7150)
  AUTHORS   Uezu,A., Kanak,D.J., Bradshaw,T.W., Soderblom,E.J., Catavero,C.M.,
            Burette,A.C., Weinberg,R.J. and Soderling,S.H.
  TITLE     Identification of an elaborate complex mediating postsynaptic
            inhibition
  JOURNAL   Science 353 (6304), 1123-1129 (2016)
   PUBMED   27609886
REFERENCE   4  (bases 1 to 7150)
  AUTHORS   Delgado,A.P., Brandao,P., Chapado,M.J., Hamid,S. and Narayanan,R.
  TITLE     Open reading frames associated with cancer in the dark matter of
            the human genome
  JOURNAL   Cancer Genomics Proteomics 11 (4), 201-213 (2014)
   PUBMED   25048349
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC022188.13.
            
            On or before Jun 16, 2020 this sequence version replaced
            XM_005254369.4, NM_001039614.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR279843.9025.1,
                                           SRR13170464.40521.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000569673.3/ ENSP00000457205.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1242              AC022188.13        66842-68083         c
            1243-1965           AC022188.13        65707-66429         c
            1966-7150           AC022188.13        50190-55374         c
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q24.1"
     gene            1..7150
                     /gene="INSYN1"
                     /gene_synonym="C15orf59"
                     /note="inhibitory synaptic factor 1"
                     /db_xref="GeneID:388135"
                     /db_xref="HGNC:HGNC:33753"
                     /db_xref="MIM:617128"
     exon            1..1242
                     /gene="INSYN1"
                     /gene_synonym="C15orf59"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1965
                     /gene="INSYN1"
                     /gene_synonym="C15orf59"
                     /inference="alignment:Splign:2.1.0"
     CDS             1810..2691
                     /gene="INSYN1"
                     /gene_synonym="C15orf59"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UPF0583 protein C15orf59; inhibitory synaptic protein 1"
                     /codon_start=1
                     /product="inhibitory synaptic factor 1 isoform 1"
                     /protein_id="NP_001034703.1"
                     /db_xref="CCDS:CCDS32289.1"
                     /db_xref="GeneID:388135"
                     /db_xref="HGNC:HGNC:33753"
                     /db_xref="MIM:617128"
                     /translation="MNIRGAPDLGQPSDDPSSGGERERIRQRMKMVIGQLEGILRELK
                     EVAKELREVVSQIDKLTSDFDFELEPDDWTTATVSSTSSSDKAGMGGPFDLGHLDFMT
                     ADILSDSWEFCSFLDVSTPSDSVDGPESTRPGAGPDYRLMNGGTPIPNGPRVETPDSS
                     SEEAFGAGPTVKSQLPQRTPGTRERVRFSDKVLYHALCCDDEEGDGEQEVEEEEVGLP
                     PEPAHTEAHAGPHKPSPAPYKSRRSPLTSRHSGSTLAPEQTRRVTRNSSTQTVSDKST
                     QTVLPYTATRQKARGKN"
     exon            1966..7150
                     /gene="INSYN1"
                     /gene_synonym="C15orf59"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2788..2793
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INSYN1"
                     /gene_synonym="C15orf59"
                     /note="hexamer: AATAAA"
     polyA_site      2811
                     /gene="INSYN1"
                     /gene_synonym="C15orf59"
                     /note="major polyA site"
     regulatory      7130..7135
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INSYN1"
                     /gene_synonym="C15orf59"
                     /note="hexamer: AATAAA"
     polyA_site      7150
                     /gene="INSYN1"
                     /gene_synonym="C15orf59"
                     /note="major polyA site"
ORIGIN      
        1 ggccccttcc ctgacagcgc cgaggcaagc ggagccaagc gagccgggcg gcctttaagg
       61 gagccagcgc ccgcccgggc ggccagtccg gcggggggcg gcggggcggg ccgggggcgg
      121 ccggggcgcg ggcggcgcgg cgccgcggcg cttggggagc gggcccaggc cccgggagcc
      181 cggcggggca gggcagggcg aggcggtgaa gggaggccca gccccgaccc ggcgcctgtt
      241 tgccccaccg gcggcgaccc ggcgcccggg ggctgctgag gcggggagcc gagagcgaac
      301 ggacgggagg tagcggagcc gagcggaggc tgcgagccta gtgcgccccg gcccggcccg
      361 gcccgccgct cgccccctcc cctcccttcc cctcgccgcg cctcctcccc tcgcctcttc
      421 tcgcctcctc tcctctcctc cgcccggcgc tgctcccgcc tgcgcccggg ccgccgaacc
      481 cagcccggcc ggggcccccg gcttcgcgcg ctctcggctg ggcccggcgg ggaccgggtc
      541 cccgcggctg acatcgcccc tcctcgggga cccggtggat tccggacctt tcggaacgtg
      601 aactgagccg tgggagcggt ctcgcccagg gccgaccgcg gggctgggct gagcagaccc
      661 tcgcggccgc tccggacgcc gcgggtgccg gggcacggac gttctgggtc cgggctgagc
      721 gcgtcccggg cgcccggcgg tgccagctca ctccgggcgg ggtagggcag ggcagacccc
      781 agcggaacac ggacccggcc gatctggact ggacagagcg aatcccggac ggtgcagagc
      841 ccggggaatg ccgggcgtgg gctgagggga tcccggcgcc ctcccagccc tccgactttg
      901 gctcacgcgc tgggacctgc ccggcgtctc ctcggcctcc ccgcgccccg gtcacctgtg
      961 gcctcggagc cccaaatctg ccggaccctc gctcattccc gaccccgagc ctggagaggc
     1021 ttgagaaaga tttttcctgc ccccttccct ggagcgcgga ggccccgccc cctcccaccg
     1081 cccagatgtc tccggtcacg gctcggagga cctccctgag gcccggggcc gaagggtcca
     1141 gagagagaag cccgccctag ggagccttgg gggcccccca actccgtctt ggggcttggc
     1201 ccccgacgct gcttggaggg aaggccggtg cccccaccct cgggggctgc cctcttccaa
     1261 tccagctccc tccccagagc tgcccatatc cggagcccct tccacatctg gaagaccctg
     1321 gccaccatcc ctctgcaggt gtcagttgca tccagagacc tctgcctttg caactgccta
     1381 ggagttgtga ggggatccct gctgccttcc cccatcccaa gggcatctct tacccttagt
     1441 ccctgcagag tcatccccct gcctctgtgt tcaagctcag gcagagatca gctctgcaca
     1501 cccacaggca cttcaagacc tggaggccac tcctgggacc caaggtccct ccccctcctc
     1561 tcctgtgtcc tcaccttgac cccctctgct agactctgtc tccctctggg tgtcccactc
     1621 tccacccaga ggcagaggag gctgggtggc agggggcagg ggctggttga gggccgccca
     1681 gggccagggt gggctggggg gccgttaaaa tgtggagctc tgcccagccg aggggggctc
     1741 cgtggaggca gacgcagtgt gtgcccagct ggggggagta tgggcaggcc tgcggccacg
     1801 tggtaaacga tgaacattcg gggcgccccg gacctcgggc agcccagtga cgaccccagc
     1861 agtggtggtg agcgggagcg gattcgacag cgcatgaaga tggtcatcgg gcagcttgag
     1921 ggcatccttc gcgagctcaa ggaggtggcc aaggagctga gggaggtggt gagccagatc
     1981 gataagctaa cctcggactt cgactttgaa ctggagccgg acgactggac cacggccact
     2041 gtgagcagca cctctagcag tgacaaggcg ggcatgggtg gcccctttga cctgggccac
     2101 ctggacttca tgacagccga tatcctctca gacagctggg agttctgctc cttcctggac
     2161 gtctctaccc cctcggactc cgtggatggt cccgagtcga ctcggccagg ggctggccct
     2221 gactaccgcc tcatgaatgg tggcacgccc atccccaatg ggccacgagt ggagacccca
     2281 gactcctcca gtgaggaggc ctttggtgct ggccccacgg tgaagagcca gctgccccag
     2341 cggaccccag ggacacggga gagggtgcgg ttcagtgaca aagtgctcta ccatgctctg
     2401 tgctgtgacg atgaggaggg ggacggtgag caggaggtgg aggaggaaga agtgggcttg
     2461 ccccctgagc ctgcccatac agaggcccat gcaggccccc acaagccctc cccagccccc
     2521 tacaagtcac ggcgctctcc actgaccagc cgccactcag gctctacctt ggcccctgaa
     2581 cagactcgaa gggtcacgag gaacagcagc acccagacag tgtcagacaa gagcacgcag
     2641 acggttctgc cctacacagc cactagacag aaagccaggg ggaaaaacta ggggccgggg
     2701 agggggccgg gggcggtggg cacatagagc tataaataaa tatatatata tatatatata
     2761 tatatatatt taaacaaatg aagtcataat aaaatttaaa aatgctaagg atctggcccc
     2821 acaggcccca aagctttcac agagctcctc ttagacatga agatgcccat tggcctccta
     2881 ggtcccagga ggtgtgggca ggactgccct tcctccgttc tcattgcggg gctcctgaag
     2941 ggggtatctg aaagtatgta aaactgatgg gaggtctgat cctccttttg ctagcccctg
     3001 aactctgtgg tggagtctgg ctgatggcca gggccatgtc ctagagggga ctcccctgca
     3061 ggaggcgggc tctaaaggga gtggtgtccc tttaggccag ggttcacagt cggggtggtc
     3121 tggagactgc aggactcagg gcctgggggt ggcatcagtc tggccaggcc ctgcgtcact
     3181 tgcacccacc atccttctac tccaaggcag acttactcgg ctatacacag tcctgcctca
     3241 cccccttcca tcttagggtt gcctgggaca taggggaatg gcaagctgct cccatggcgc
     3301 aggcctccgt gctgggatgc tgggcatcca cagagcctga aggatcgcct gggctgggca
     3361 ggggtttgat tggagggtct ggaccacccc caatcctttc agctcccatt ctcagcctga
     3421 ttctgagacc aagaccaaaa cctcagggca aagcctcagc cagtcctggc gtggccctaa
     3481 gcagacctga gatctgtgcg aggaagtagg cagccacttc catggggcta aaaattaggg
     3541 ccgtagacag gctcctggcc accttcacca ggtgggcagc cttggaaagg agcccagtgg
     3601 tccccactcc gtcccgatgt ctccacaccc agcccctgag cccatctcag caggtcctga
     3661 acaaagatga ggttacagcc tagaggtacc agggcttcat ctggggcatg gccagcacag
     3721 ggacagcagc tgaatatcct tgggataata atgacaatta tattgctcac atttattgag
     3781 caggtctcta tgctacttct atgccgaata ctttttatag cagcatctca tttaatcacc
     3841 acaataacct gatgagacag gaagtattat taccccattt ttctttttct ttctttcttt
     3901 ttttattttt atttttttta tttttgagat ggagtctcgc tctgtcaccc aggctggagt
     3961 gcagtggggc gatcttggct cactgcaacc tccacctgcc aggttcaagc aattctccca
     4021 cttcagcctc cagagcaact gggactacag gcatgcgtca ccacgcctag ctaatttttg
     4081 tatttttagt agagacgggg tttcaccatg ttgggcaggc tggtctgaac tcctgacctc
     4141 aagtgattcg tcagcctcag cctcccaaag tcccgggatt acaggcgtga gccactgtgc
     4201 ccccacctat ccccattttt caaatgagat gactgaggtt caaagtagtt cagtacctgc
     4261 cccaaagcca caaagcctgt gctactctgc ccccaagaat tagtgatttc caggctgtgc
     4321 ctggctctgc tagctagcag ctgtgtgacc ttggcaagtc cgtttgcctc tctgagccta
     4381 tctacctcct ctgtataatg ggtctggtag tcttcaccta cctcaccagc tgctgtggag
     4441 ctccgacgag atggtgactg tgaaagcact tgacaaactg aaggcactgg acatgccttg
     4501 tggtcagtgt ggcctcaacc cagatgtcca gtgatttcca gggcacaggg gctttgagtg
     4561 ggatggccaa agaagacacc tccatccaac tgggagccac ccctgggtca caaacactcc
     4621 attgcttggt ccctcgcagt agctcgaggt cagaagttga ctgcagcttg attcacagcc
     4681 cctcgctcag agcagggagg tgggactggc agcacagcaa agacatcgct ccttgggggc
     4741 ctccctgttg catatttcaa ttaaggcagg tttacagccc cccagcgtct gtccggaggg
     4801 ggcctgagca gcaggcctgg cagcacccac tgctcctgcc tgaagaggtg ctatccagcc
     4861 ccggctgtgg acatacagtg agattgtaca ggccggtctg gagcagctgc aggggatatg
     4921 atctggataa gactgagcag acctaggagg ctctgcaatg tagagcttat tcctcaagct
     4981 catggtgaga ttggagccct aaagagttag ccagggtgca gccaccagtg tggaaatcca
     5041 gggatggcgc aggccatggc cagctcttcc cagctcacgt ctgagaacca agggtctgag
     5101 cccctctatc agccccagga aatcctcagc catttggtgc tgatcagcga tagggctctg
     5161 gttcacagga tgagtgccag gggctcctct ggggagaggg gccagctgca ttccgcccca
     5221 ccctgagagt gagaaggggg cagtccccga ccaggaatgg gcctacctgg tgctaagaat
     5281 ggacataaca gttctccctc tgaggcttgc atttcatctg tcatggcaag agcactttca
     5341 ggctcacagt ttctcatctt ttcaacagtc agggaaaaga aacctatgga atagttcgca
     5401 tttcacagag gaagaaactg agggccagag ctaggggtct ctgacacagc catgggatcc
     5461 tgcaccccac actggctcta tgtgactctg tagtagtggc taatgtccat cagtcgcccc
     5521 ctgctcccct gcctggccat ttgcccccaa atagggcagt ggtgggagta tatcctggag
     5581 gagggggaag tgggatatag gtagtacttg gctgacttca caattttgct acacccagtc
     5641 tggacctcct gacagtggag tgggatccct gtggcttcct ttttcttgtt tttgttttgt
     5701 tttgtttttt tttagatgga gtctccctct atcatccagg ctggagtgca atggtgcgat
     5761 ttcagctcac tgcaacttcc acctcccagg ttcaaacaat tctcctgtct cagccacctg
     5821 agtagcttgg actacaggca cccgccacca ccccagctac taagttttgt atttttagta
     5881 gagatggggt ttcaccacat ggccaggatg gtcttgatct cttgactttg tgatctgccc
     5941 accttggcct cgcaaagcac tgggattaca ggcgtgggcc actacgcctg gccaattttt
     6001 ttttgttttg tttttttgag atggagtctt gctccatctc ccaggctgga gagcaatggc
     6061 gtgatctcag ctcactgcaa cctctgcctc ctgggttcaa gcgattctcc tgcctcagcc
     6121 tcccaagtag ctgggattac aggcacccac catcatgccc gggttaattt ttgtattttc
     6181 ttagagatgg agtttcacta tgttggccag cctcatcttt gaactcctga cctccgatga
     6241 tccacctgcc ttggcctccc aaagtgatgg gattacaggc gtgagccacc gcacctggcc
     6301 ccagtggctt cttcagactt gaaacacaaa atgtggccag ctagggatag agagaattct
     6361 gactttcaac actgctgagt catggcatgg gctgcttctg ggtagtgagc tccctgtcct
     6421 tggggtagac acagccatcc cttgggtcct tcctccagac cttccaggta cagccctttg
     6481 ctgctcttcc ctgcctccaa acctttgcca gtttttgagt ttctttaccc aggatgttcc
     6541 atcagatctc tctgcttccg ggaagtccta ttccactgac cacctactgt gtgcacaggc
     6601 ttgtgctgag tggtgctgtg gaggggcaga agggagctgg aacctggtga tggagagaag
     6661 tcatagcatg atgaataact catgtccact gggcacgtgc taggcactgg gcattgttct
     6721 aagtgtatgt cttatctcat ttactcctca cagcacgtat gagataagca atcttactta
     6781 tgtctaagta gcttccagat gaggaaactg aggctctggg aagtgaagta acttgcctaa
     6841 ggaaacacag gatgggctat ggaaccagga ttcaaccaca aacacagtga cttagcatca
     6901 tctactgcaa acatccactg cagttaaaat gcctggagtg ggtggcctgg tcactggagg
     6961 tgagggtcgg ggctgggtcg tctgagagcc aggcgggtct tcggggtgag gggagtgttg
     7021 gggtgaggaa acatgtgaac atgcctcagt ctttggagaa cttagttact gttaacctga
     7081 atgtcaccac ccctgactcg gagactggca ccaaatggat tatgggttca ataaatgttt
     7141 gttgaatgaa
//
