LOCUS       NM_016133               3562 bp    mRNA    linear   PRI 17-MAR-2024
DEFINITION  Homo sapiens insulin induced gene 2 (INSIG2), transcript variant 1,
            mRNA.
ACCESSION   NM_016133
VERSION     NM_016133.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Fukuoka,M., Kodama,T., Murai,K., Hikita,H., Sometani,E., Sung,J.,
            Shimoda,A., Shigeno,S., Motooka,D., Nishio,A., Furuta,K.,
            Tatsumi,T., Yusa,K. and Takehara,T.
  TITLE     Genome-wide loss-of-function genetic screen identifies INSIG2 as
            the vulnerability of hepatitis B virus-integrated hepatoma cells
  JOURNAL   Cancer Sci 115 (3), 859-870 (2024)
   PUBMED   38287498
  REMARK    GeneRIF: Genome-wide loss-of-function genetic screen identifies
            INSIG2 as the vulnerability of hepatitis B virus-integrated
            hepatoma cells.
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Qian,Y., Li,X., Qiu,J. and Zhang,R.
  TITLE     Insulin-induced gene 2 expression is associated with cervical
            adenocarcinoma malignant behavior
  JOURNAL   Mol Biol Rep 50 (2), 1553-1563 (2023)
   PUBMED   36515824
  REMARK    GeneRIF: Insulin-induced gene 2 expression is associated with
            cervical adenocarcinoma malignant behavior.
REFERENCE   3  (bases 1 to 3562)
  AUTHORS   Watanabe,Y., Sasaki,T., Miyoshi,S., Shimizu,M., Yamauchi,Y. and
            Sato,R.
  TITLE     Insulin-induced genes INSIG1 and INSIG2 mediate oxysterol-dependent
            activation of the PERK-eIF2alpha-ATF4 axis
  JOURNAL   J Biol Chem 297 (2), 100989 (2021)
   PUBMED   34298014
  REMARK    GeneRIF: Insulin-induced genes INSIG1 and INSIG2 mediate
            oxysterol-dependent activation of the PERK-eIF2alpha-ATF4 axis.
REFERENCE   4  (bases 1 to 3562)
  AUTHORS   Jimeno,N., Velasco-Gonzalez,V., Fierro,I., Duran,M. and Carvajal,A.
  TITLE     Association of CNR1 and INSIG2 polymorphisms with
            antipsychotics-induced weight gain: a prospective nested
            case-control study
  JOURNAL   Sci Rep 11 (1), 15304 (2021)
   PUBMED   34315947
  REMARK    GeneRIF: Association of CNR1 and INSIG2 polymorphisms with
            antipsychotics-induced weight gain: a prospective nested
            case-control study.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3562)
  AUTHORS   Yan,R., Cao,P., Song,W., Li,Y., Wang,T., Qian,H., Yan,C. and Yan,N.
  TITLE     Structural basis for sterol sensing by Scap and Insig
  JOURNAL   Cell Rep 35 (13), 109299 (2021)
   PUBMED   34192549
  REMARK    GeneRIF: Structural basis for sterol sensing by Scap and Insig.
REFERENCE   6  (bases 1 to 3562)
  AUTHORS   Sun,L.P., Li,L., Goldstein,J.L. and Brown,M.S.
  TITLE     Insig required for sterol-mediated inhibition of Scap/SREBP binding
            to COPII proteins in vitro
  JOURNAL   J Biol Chem 280 (28), 26483-26490 (2005)
   PUBMED   15899885
REFERENCE   7  (bases 1 to 3562)
  AUTHORS   Dobrosotskaya,I.Y., Goldstein,J.L., Brown,M.S. and Rawson,R.B.
  TITLE     Reconstitution of sterol-regulated endoplasmic reticulum-to-Golgi
            transport of SREBP-2 in insect cells by co-expression of mammalian
            SCAP and Insigs
  JOURNAL   J Biol Chem 278 (37), 35837-35843 (2003)
   PUBMED   12842885
  REMARK    GeneRIF: Required for sterol-regulated transport of SREBPs from ER
            to Golgi.
REFERENCE   8  (bases 1 to 3562)
  AUTHORS   Yabe,D., Komuro,R., Liang,G., Goldstein,J.L. and Brown,M.S.
  TITLE     Liver-specific mRNA for Insig-2 down-regulated by insulin:
            implications for fatty acid synthesis
  JOURNAL   Proc Natl Acad Sci U S A 100 (6), 3155-3160 (2003)
   PUBMED   12624180
REFERENCE   9  (bases 1 to 3562)
  AUTHORS   Yabe,D., Xia,Z.P., Adams,C.M. and Rawson,R.B.
  TITLE     Three mutations in sterol-sensing domain of SCAP block interaction
            with insig and render SREBP cleavage insensitive to sterols
  JOURNAL   Proc Natl Acad Sci U S A 99 (26), 16672-16677 (2002)
   PUBMED   12482938
REFERENCE   10 (bases 1 to 3562)
  AUTHORS   Yabe,D., Brown,M.S. and Goldstein,J.L.
  TITLE     Insig-2, a second endoplasmic reticulum protein that binds SCAP and
            blocks export of sterol regulatory element-binding proteins
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 12753-12758 (2002)
   PUBMED   12242332
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY053967.1, AF527632.1,
            AC009303.3 and AW264638.1.
            
            On Nov 23, 2018 this sequence version replaced NM_016133.3.
            
            Summary: The protein encoded by this gene is highly similar to the
            protein product encoded by gene INSIG1. Both INSIG1 protein and
            this protein are endoplasmic reticulum proteins that block the
            processing of sterol regulatory element binding proteins (SREBPs)
            by binding to SREBP cleavage-activating protein (SCAP), and thus
            prevent SCAP from escorting SREBPs to the Golgi. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) and variant 2 both encode the
            same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.75794.1,
                                           SRR1660803.48295.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000245787.9/ ENSP00000245787.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 HY053967.1         56-58
            4-1500              AF527632.1         6-1502
            1501-1501           AC009303.3         178626-178626
            1502-1951           AF527632.1         1504-1953
            1952-1952           AC009303.3         179077-179077
            1953-2432           AF527632.1         1955-2434
            2433-2451           AC009303.3         179558-179576
            2452-2583           AF527632.1         2454-2585
            2584-2596           AW264638.1         18-30               c
            2597-3562           AC009303.3         179722-180687
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q14.1-q14.2"
     gene            1..3562
                     /gene="INSIG2"
                     /gene_synonym="INSIG-2"
                     /note="insulin induced gene 2"
                     /db_xref="GeneID:51141"
                     /db_xref="HGNC:HGNC:20452"
                     /db_xref="MIM:608660"
     exon            1..71
                     /gene="INSIG2"
                     /gene_synonym="INSIG-2"
                     /inference="alignment:Splign:2.1.0"
     exon            72..453
                     /gene="INSIG2"
                     /gene_synonym="INSIG-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..887
                     /gene="INSIG2"
                     /gene_synonym="INSIG-2"
                     /note="isoform a is encoded by transcript variant 1;
                     insulin induced protein 2; INSIG2 membrane protein;
                     insulin-induced gene 2 protein"
                     /codon_start=1
                     /product="insulin-induced gene 2 protein isoform a"
                     /protein_id="NP_057217.2"
                     /db_xref="CCDS:CCDS2122.1"
                     /db_xref="GeneID:51141"
                     /db_xref="HGNC:HGNC:20452"
                     /db_xref="MIM:608660"
                     /translation="MAEGETESPGPKKCGPYISSVTSQSVNLMIRGVVLFFIGVFLAL
                     VLNLLQIQRNVTLFPPDVIASIFSSAWWVPPCCGTASAVIGLLYPCIDRHLGEPHKFK
                     REWSSVMRCVAVFVGINHASAKVDFDNNIQLSLTLAALSIGLWWTFDRSRSGFGLGVG
                     IAFLATVVTQLLVYNGVYQYTSPDFLYVRSWLPCIFFAGGITMGNIGRQLAMYECKVI
                     AEKSHQE"
     exon            454..578
                     /gene="INSIG2"
                     /gene_synonym="INSIG-2"
                     /inference="alignment:Splign:2.1.0"
     exon            579..745
                     /gene="INSIG2"
                     /gene_synonym="INSIG-2"
                     /inference="alignment:Splign:2.1.0"
     exon            746..845
                     /gene="INSIG2"
                     /gene_synonym="INSIG-2"
                     /inference="alignment:Splign:2.1.0"
     exon            846..3562
                     /gene="INSIG2"
                     /gene_synonym="INSIG-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2568..2573
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INSIG2"
                     /gene_synonym="INSIG-2"
                     /note="hexamer: ATTAAA"
     polyA_site      2591
                     /gene="INSIG2"
                     /gene_synonym="INSIG-2"
                     /note="major polyA site"
     regulatory      3541..3546
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INSIG2"
                     /gene_synonym="INSIG-2"
                     /note="hexamer: AATAAA"
     polyA_site      3562
                     /gene="INSIG2"
                     /gene_synonym="INSIG-2"
                     /note="major polyA site"
ORIGIN      
        1 gttgtgggag tggaggagga agaggcggta gggggtacgg gggctggtcc cagaagatgg
       61 cggaggcggg ggatttctgg taggtcctac tttaggacaa gatgtggtac cgttgaagcg
      121 tcagtctttg attcacagac agttgagctt ttcagctggg aagcctttcc attttttttt
      181 ttttaacggc tttctgaacc tatgaaacca tggcagaagg agagacagag tcacctgggc
      241 ccaaaaagtg tggcccatat atttcatctg tcactagcca gagtgtgaac ttgatgattc
      301 gaggagtagt gctatttttt attggagtat ttcttgcatt agtgttaaat ttacttcaga
      361 ttcagagaaa tgtgacgctc tttccacctg atgtgattgc aagcatcttt tcttctgcat
      421 ggtgggtacc cccatgctgt ggcacggctt cagctgtgat tgggttatta tacccctgca
      481 ttgacagaca tctaggagaa ccacataaat ttaaaagaga gtggtccagt gtaatgcggt
      541 gtgtagcagt ctttgttggt ataaatcatg ccagtgctaa agtggatttc gataacaaca
      601 tacagttgtc tctcacactg gctgcactat ccattggact gtggtggact tttgatagat
      661 ctagaagtgg ttttggcctt ggagtaggaa ttgccttctt ggcaactgtg gtcactcaac
      721 tgctagtata taatggtgtt taccaatata catctccaga tttcctctat gttcgttctt
      781 ggttaccatg tatatttttt gctggaggca taacaatggg aaacattggt cgacaactgg
      841 caatgtacga atgtaaagtt atcgcagaaa aatctcatca ggaatgaaga aggcaaaaaa
      901 tatcttttgt acagaaaagc aagatgaaaa ggatgtgaaa tggtagatat accaacaaaa
      961 cttcagactg taaaattgcc aggatgcagt tttccccttg attggcgtgt gtgtatatat
     1021 ggataaatat atatatacac acacacatat tactgcaatc tgtgattgct tcatctgtaa
     1081 atcagttgta aacctttaca tatttgactt aaataactgt aagatatata tgtactacat
     1141 taaaaagtgt tgattaatag atgaaatttt taaattaatt ttttaaaaca tgccatacat
     1201 tgtatcacaa tgttaatgtg ccaagatatt gttcctgtca tgcagagtat aagaatgctt
     1261 tgaacaattt gtagacttag tgaaataaaa taagaggaaa gccaaaaaca aacaaacaaa
     1321 aagcatatgg ggagctggta ttttctcttt agcttactgt tgtgcctttt tatttttcta
     1381 atcacagcag tatgagttat gagtgcccta atttgtggtt agtttctaat ttaatgttgt
     1441 ttcatagagt ttggagtgtt ttgatacagg gtgaaaatga acttctggtt tcaaacctgc
     1501 gttactggag acagcccaaa gagtaatttt ctgttttgac aggttttact ggaagtatat
     1561 gtgatgagca gaagaggtta tcagcattaa attgttttgg ttctaaattt ggaacagtat
     1621 atataattaa aagtaaggaa cattagagga tttaattaga ataaatacat gttttggaaa
     1681 tacagtgacc tcttgcagtg tcacaaaagt gcaaagtgat attagctgtc atctgcaata
     1741 cagaatctca ttgcttttgc acatggagca tataggaaac tccaaacaga tcacaatgag
     1801 gtttctaaat ctgttgggtt ctgtcttcta ttgggttctg tgaagcaaac cactgtagct
     1861 ttagctgggt tcagtcatat gactcgttgg tggaatgcct aggtttttca tcttacatgc
     1921 agtcttgggg gtggatgaat acataatttc tcatgtattc gtgtatccat tagtgaatag
     1981 ttcaagtctg tttaagagtg tattgagatg gcattctctg catgttaaag atcttaatgg
     2041 caaccagcac ctcttaagta tggtttaaac atattcttag ctaatttttt ccattagttt
     2101 ttgaaattgg tggcagttgt ctgatccaca agggcaagat cttctgagta ctctggggtg
     2161 tgagtatgtg tgcacacgtg tgtgttggag tgagtgagag aatgtgtctg tgcatgtggc
     2221 catgctttcc tagaatgtca agtagatatt tttacacttt gagttttaaa gcaattacta
     2281 tcagactgag atcttgtatg ccaaacttta atctgctttt atgttttcag gctgaaggtg
     2341 tgaaaatcct aagaggattt catattgaat atgtgtacac aatcttaact atcgtggtgg
     2401 aaaacatact actataattt attattatat cttccagata atgttattca tttagaacaa
     2461 ataaggtata ttttttagaa tcaactttgt aagcactata aaatctttaa taagttataa
     2521 ggtctatgat gtgtttactt taaaaattgc tgttaaaagc aacacgtatt aaatatgtaa
     2581 ttatcatctg ggttaagagt ctgtttttct tctttgtggt aagtcttaga atatggtact
     2641 gtggattaat ctaatgaaat taacatatgt ggttgaagtt accaagaaac gatgaaaaga
     2701 aactaaatat agttgaccct tgaacaacag gagttagggg caccactccc caacatagtt
     2761 gaaaatccat gtataacttt tgactcctcc aaaacttaac tactaatagc ctactcttga
     2821 tgggaagcct taccaataag aaacagttga tgaacacata ttgtgtatgg tatatgtatt
     2881 atatactgtt ttcttacaat agtgtaagct aaggaaaaga aaatgtgttt actcttcatt
     2941 cagtggaagt ggaccatcct aaaagtcttc actcatgcta tcttcatgct gagtagtagg
     3001 ctgaggagga ggaggaggag gggttggtct tgctgttgcg ggggtggaag aagatgtgca
     3061 ttattagtgg gcccacacag ctgaaaacca tgctgttcaa gggtcaactg tgtgtggtat
     3121 cggtgagact gaatgattat cgttggtgtt cttaaattat tttcaggtcc tctagaaaat
     3181 aagataatta tagcaaaaaa taaaattgtt ttgaaatttt gataaatata taaatgtata
     3241 gaacctattt ttttcaatga aagaaaatgg gaaacatttt ttaacattgc tagattggca
     3301 gtgctttctt ttattatttc aagttatgga aatttgcaca gttgaaaact ggggataaat
     3361 tctacaaaag tgcaagaaaa caattcaaat tagccagagc cctgctaaaa cttttaatgt
     3421 aaaatttatt tatttgcact gagctgtttt accttcttta aggccattgg gacctctcaa
     3481 tgatgcatat gtttcataca tcttgtattt tccaggtaga agtcagactt ggttaatacc
     3541 aataaatact tctgtaatcc ta
//
