LOCUS       NM_001351426            9910 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens inositol polyphosphate-4-phosphatase type I A
            (INPP4A), transcript variant g, mRNA.
ACCESSION   NM_001351426 XM_017003997
VERSION     NM_001351426.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Xu,Y., Tong,X., Liu,P., Huang,J., Chen,S., Liu,D., Gu,T., Xie,Y.,
            Guo,D. and Xu,Y.
  TITLE     Deficiency of INPP4A promotes M2 macrophage polarization in
            eosinophilic chronic rhinosinusitis with nasal polyps
  JOURNAL   Inflamm Res 73 (4), 581-595 (2024)
   PUBMED   38363325
  REMARK    GeneRIF: Deficiency of INPP4A promotes M2 macrophage polarization
            in eosinophilic chronic rhinosinusitis with nasal polyps.
REFERENCE   2  (bases 1 to 9910)
  AUTHORS   Maruyama,N., Umikawa,M., Matsumoto,H., Maruyama,T., Nishihara,K.,
            Nakasone,T., Matayoshi,A., Goto,T., Hirano,F., Arasaki,A.,
            Nakamura,H., Matsuzaki,G. and Takaesu,G.
  TITLE     miR-935 Inhibits Oral Squamous Cell Carcinoma and Targets Inositol
            Polyphosphate-4-phosphatase Type IA (INPP4A)
  JOURNAL   Anticancer Res 40 (11), 6101-6113 (2020)
   PUBMED   33109548
  REMARK    GeneRIF: miR-935 Inhibits Oral Squamous Cell Carcinoma and Targets
            Inositol Polyphosphate-4-phosphatase Type IA (INPP4A).
REFERENCE   3  (bases 1 to 9910)
  AUTHORS   Banihashemi,S., Tahmasebi-Birgani,M., Mohammadiasl,J. and
            Hajjari,M.
  TITLE     Whole exome sequencing identified a novel nonsense INPP4A mutation
            in a family with intellectual disability
  JOURNAL   Eur J Med Genet 63 (4), 103846 (2020)
   PUBMED   31978615
  REMARK    GeneRIF: Whole exome sequencing identified a novel nonsense INPP4A
            mutation in a family with intellectual disability.
REFERENCE   4  (bases 1 to 9910)
  AUTHORS   Khanna,K., Chaudhuri,R., Aich,J., Pattnaik,B., Panda,L.,
            Prakash,Y.S., Mabalirajan,U., Ghosh,B. and Agrawal,A.
  TITLE     Secretory Inositol Polyphosphate 4-Phosphatase Protects against
            Airway Inflammation and Remodeling
  JOURNAL   Am J Respir Cell Mol Biol 60 (4), 399-412 (2019)
   PUBMED   30335467
  REMARK    GeneRIF: INPP4A is secreted by airway epithelial cells and
            extracellular INPP4A critically inhibits airway inflammation and
            remodeling.
REFERENCE   5  (bases 1 to 9910)
  AUTHORS   Huang,Y., Xiao,W., Jiang,X. and Li,H.
  TITLE     MicroRNA-935 acts as a prognostic marker and promotes cell
            proliferation, migration, and invasion in colorectal cancer
  JOURNAL   Cancer Biomark 26 (2), 229-237 (2019)
   PUBMED   31524145
  REMARK    GeneRIF: Low INPP4A expression is associated with cell
            proliferation, migration, and invasion in colorectal cancer.
REFERENCE   6  (bases 1 to 9910)
  AUTHORS   Munday,A.D., Norris,F.A., Caldwell,K.K., Brown,S., Majerus,P.W. and
            Mitchell,C.A.
  TITLE     The inositol polyphosphate 4-phosphatase forms a complex with
            phosphatidylinositol 3-kinase in human platelet cytosol
  JOURNAL   Proc Natl Acad Sci U S A 96 (7), 3640-3645 (1999)
   PUBMED   10097090
REFERENCE   7  (bases 1 to 9910)
  AUTHORS   Zhang,X. and Majerus,P.W.
  TITLE     Phosphatidylinositol signalling reactions
  JOURNAL   Semin Cell Dev Biol 9 (2), 153-160 (1998)
   PUBMED   9599410
  REMARK    Review article
REFERENCE   8  (bases 1 to 9910)
  AUTHORS   Norris,F.A., Atkins,R.C. and Majerus,P.W.
  TITLE     The cDNA cloning and characterization of inositol polyphosphate
            4-phosphatase type II. Evidence for conserved alternative splicing
            in the 4-phosphatase family
  JOURNAL   J Biol Chem 272 (38), 23859-23864 (1997)
   PUBMED   9295334
REFERENCE   9  (bases 1 to 9910)
  AUTHORS   Norris,F.A., Atkins,R.C. and Majerus,P.W.
  TITLE     Inositol polyphosphate 4-phosphatase is inactivated by
            calpain-mediated proteolysis in stimulated human platelets
  JOURNAL   J Biol Chem 272 (17), 10987-10989 (1997)
   PUBMED   9110986
REFERENCE   10 (bases 1 to 9910)
  AUTHORS   Norris,F.A., Auethavekiat,V. and Majerus,P.W.
  TITLE     The isolation and characterization of cDNA encoding human and rat
            brain inositol polyphosphate 4-phosphatase
  JOURNAL   J Biol Chem 270 (27), 16128-16133 (1995)
   PUBMED   7608176
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010134.5.
            
            On Aug 13, 2020 this sequence version replaced NM_001351426.1.
            
            Summary: This gene encodes an Mg++ independent enzyme that
            hydrolyzes the 4-position phosphate from the inositol ring of
            phosphatidylinositol 3,4-bisphosphate, inositol
            1,3,4-trisphosphate, and inositol 3,4-bisphosphate. Multiple
            transcript variants encoding distinct isoforms have been described.
            [provided by RefSeq, Aug 2008].
            
            Transcript Variant: This variant (g), as well as variant f, encodes
            isoform f.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN03465402,
                              SAMN03465403 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               AC010134.5         32783-32923
            142-203             AC010134.5         105737-105798
            204-412             AC010134.5         106719-106927
            413-457             AC010134.5         107460-107504
            458-576             AC010134.5         120150-120268
            577-693             AC010134.5         122502-122618
            694-773             AC010134.5         122902-122981
            774-885             AC010134.5         124636-124747
            886-976             AC010134.5         125664-125754
            977-1124            AC010134.5         126301-126448
            1125-1255           AC010134.5         130650-130780
            1256-1360           AC010134.5         132742-132846
            1361-1469           AC010134.5         133359-133467
            1470-1487           AC010134.5         135725-135742
            1488-1671           AC010134.5         139559-139742
            1672-1890           AC010134.5         141044-141262
            1891-2029           AC010134.5         142326-142464
            2030-2062           AC010134.5         146236-146268
            2063-2235           AC010134.5         150238-150410
            2236-2359           AC010134.5         151413-151536
            2360-2486           AC010134.5         152413-152539
            2487-2627           AC010134.5         152802-152942
            2628-2725           AC010134.5         155344-155441
            2726-2838           AC010134.5         159588-159700
            2839-2993           AC010134.5         163762-163916
            2994-9910           AC010134.5         174249-181165
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q11.2"
     gene            1..9910
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /note="inositol polyphosphate-4-phosphatase type I A"
                     /db_xref="GeneID:3631"
                     /db_xref="HGNC:HGNC:6074"
                     /db_xref="MIM:600916"
     exon            1..141
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            142..203
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            204..412
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             307..3126
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /EC_number="3.1.3.66"
                     /note="isoform f is encoded by transcript variant g; type
                     I inositol 3,4-bisphosphate 4-phosphatase; inositol
                     polyphosphate-4-phosphatase, type I, 107kD; inositol
                     polyphosphate-4-phosphatase, type I, 107kDa"
                     /codon_start=1
                     /product="inositol polyphosphate-4-phosphatase type I A
                     isoform f"
                     /protein_id="NP_001338355.1"
                     /db_xref="GeneID:3631"
                     /db_xref="HGNC:HGNC:6074"
                     /db_xref="MIM:600916"
                     /translation="MTAREHSPRHGARARAMQRASTIDVAADMLGLSLAGNIQDPDEP
                     ILEFSLACSELHTPSLDRKPNSFVAVSVTTPPQAFWTKHAQTEIIEGTNNPIFLSSIA
                     FFQDSLINQMTQVKLSVYDVKDRSQGTMYLLGSGTFIVKDLLQDRHHRLHLTLRSAES
                     DRVGNITVIGWQMEEKSDQRPPVTRSVDTVNGRMVLPVDESLTEALGIRSKYASLRKD
                     TLLKSVFGGAICRMYRFPTTDGNHLRILEQMAESVLSLHVPRQFVKLLLEEDAARVCE
                     LEELGELSPCWESLRRQIVTQYQTIILTYQENLTDLHQYRGPSFKASSLKADKKLEFV
                     PTNLHIQRMRVQDDGGSDQNYDIVTIGAPAAHCQGFKSGGLRKKLHKFEETKKHSFEE
                     CCTSSGCQSIIYIPQDVVRAKEIIAQINTLKTQVSYYAERLSRAAKDRSATGLERTLA
                     ILADKTRQLVTVCDCKLLANSIHGLNAARPDYIASKASPTSTEEEQVMLRNDQDTLMA
                     RWTGRNSRSSLQVDWHEEEWEKVWLNVDKSLECIIQRVDKLLQKERLHGEGCEDVFPC
                     AGSCTSKKDCSPPPEESSPGEWSEALYPLLTTLTDCVAMMSDKAKKAMVFLLMQDSAP
                     TIATYLSLQYRRDVVFCQTLTALICGFIIKLRNCLHDDGFLRQLYTIGLLAQFESLLS
                     TYGEELAMLEDMSLGIMDLRNVTFKVTQATSSASADMLPVITGNRDGFNVRVPLPGPL
                     FDALPREIQSGMLLRVQPVLFNVGINEQQTLAERFGDTSLQEVINVESLVRLNSYFEQ
                     FKEVLPEDCLPRSRSQTCLPELLRFLGQNVHARKNKNVDILWQAAEICRRLNGVRFTS
                     CKSAKDRTAMSVTLEQCLILQHEHGMAPQVFTQALECMRSEGCRRENTMKNVGSRKYA
                     FNSLQLKAFPKHYRPPEGTYGKVET"
     exon            413..457
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            458..576
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            577..693
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            694..773
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            774..885
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            886..976
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1124
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1255
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1360
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1469
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1487
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1488..1671
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1890
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..2029
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2030..2062
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2235
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2236..2359
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..2486
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2487..2627
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2628..2725
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2726..2838
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2839..2993
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2994..9910
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5650..5655
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /note="hexamer: AATAAA"
     polyA_site      5674
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /note="major polyA site"
     regulatory      6280..6285
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /note="hexamer: ATTAAA"
     polyA_site      6304
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /note="major polyA site"
     polyA_site      6847
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
     regulatory      9566..9571
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /note="hexamer: AATAAA"
     polyA_site      9583
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
     regulatory      9892..9897
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
                     /note="hexamer: ATTAAA"
     polyA_site      9910
                     /gene="INPP4A"
                     /gene_synonym="INPP4; TVAS1"
ORIGIN      
        1 agagaaattc tgactggtcc atctgttcac agtgggttgt gtggaccatg gggaagccag
       61 tggggttgag gacagagtgg gttgacttcg gtgtgaatca tggttctgcc gcttgctgac
      121 tctggttctt atctctaaaa gatgatggat ttggacatgc ttctatgaag aaatgccaca
      181 tggtccgaga gcaaacatgt ccaggctact gccactttag tggactaggg ctcggtgcca
      241 gcacttcccg ggtaatcagg cgtggtctga ccgaggatca agaagcacat catcaccaat
      301 gacatcatga cagcaagaga gcacagccct cgccatggtg ccagggcccg tgcaatgcag
      361 cgggcttcca ccatcgacgt ggcggccgac atgctgggcc tctctctggc aggaaatata
      421 caagacccag atgagcccat tttagaattt agcttagctt gcagtgagct gcatactcca
      481 tcgctagatc gaaagccaaa tagttttgtt gcggtgagtg tcaccacccc tcctcaggca
      541 ttctggacga agcatgcaca gacggagatc attgagggaa ccaacaatcc tatatttcta
      601 agcagtattg ccttctttca agactctctt atcaatcaga tgacacaagt caaactctcc
      661 gtgtatgatg tcaaagatag atctcaggga acaatgtatt tactgggctc tggaacgttc
      721 attgtcaaag atctgctcca ggacaggcat cataggttgc atttaacact aaggtctgca
      781 gagagtgacc gtgtaggtaa catcaccgtg attggctggc agatggagga gaagtcagac
      841 caacggcccc ctgtgacccg gtctgtggac actgtcaatg ggaggatggt tcttcctgtc
      901 gatgagagct tgacggaggc gttaggaatc cgatccaaat acgcttcatt gcgaaaggac
      961 actttgctga aatcggtgtt cggtggtgcc atctgccgca tgtaccggtt tccaaccact
     1021 gatggtaacc atttgcggat cctggagcag atggcagaga gcgtgctctc cctgcacgtg
     1081 ccccggcagt tcgtgaagct cctactagag gaagatgcag ccagagtgtg tgagctggag
     1141 gagctgggag agctgtcccc ttgctgggag agcctccggc gccaaattgt cacccagtac
     1201 cagaccatca tcctcacata ccaggagaac ctgaccgacc tccatcagta cagagggccc
     1261 tcgtttaaag caagcagttt gaaagcagat aaaaagttag aatttgttcc cacaaacttg
     1321 cacatacaaa ggatgagagt tcaagacgat ggaggatcag atcagaacta cgacatcgtc
     1381 accattgggg cgccagcagc acactgccaa ggttttaagt caggaggtct ccgcaaaaag
     1441 ctgcacaaat ttgaagagac caagaaacac agttttgagg agtgttgtac atcatctggc
     1501 tgccagtcca taatctacat accccaggat gttgtcagag ccaaggagat catcgcccag
     1561 atcaacaccc tgaaaaccca agtgagttac tacgcagagc ggctgtcaag ggcagccaag
     1621 gacaggtctg ccactggcct tgagaggaca ctcgccatct tggcagacaa gacacggcag
     1681 ctggtcacgg tctgcgactg caagctcctg gccaactcca tccatgggct gaacgctgca
     1741 cggcctgact acattgcctc caaggcctct cccacttcga ctgaggagga gcaggtgatg
     1801 cttagaaatg accaggacac cctcatggcc cggtggacag ggagaaacag ccgatcttcc
     1861 ctgcaggtgg actggcacga ggaggagtgg gagaaagtgt ggctgaacgt ggacaagagc
     1921 ctagagtgca tcattcagcg tgtggacaag ctgctgcaga aggagcggct gcatggcgag
     1981 ggctgtgagg atgtcttccc ctgtgcaggc agctgcacca gcaagaaaga ttgcagtccc
     2041 cctcctgaag agtccagccc aggtgaatgg agtgaggccc tttacccgct gctgaccact
     2101 ctcaccgact gcgtggccat gatgagtgac aaggccaaga aggccatggt attcctgctc
     2161 atgcaggaca gcgcgcccac catagccacc tacctgagcc tgcagtaccg ccgtgacgtg
     2221 gtcttctgcc agacgctgac cgccctcatc tgcggcttca tcattaagct gaggaactgc
     2281 ctgcatgacg acggcttcct gcgccagctc tacaccatcg ggctgctggc ccagttcgag
     2341 agcctgctga gcacctacgg ggaggagctg gcaatgctgg aggacatgag ccttgggatc
     2401 atggacttga ggaacgtgac cttcaaagtc actcaggcca cttccagcgc ctccgcagac
     2461 atgctgcccg tcatcacagg aaatcgcgac gggtttaacg tgcgggtccc tctgccgggc
     2521 ccgctgtttg acgccttgcc ccgggagatc cagagtggca tgctgctgcg agtgcagccc
     2581 gtcctcttca acgtgggcat caatgagcag cagacactgg ccgagaggtt tggcgatacg
     2641 tctttacaag aagtcatcaa cgtggagagt ttggtgcggt taaattccta ctttgagcag
     2701 tttaaggaag ttttgcctga ggattgcctg cctcggtctc gcagtcagac gtgcctgcca
     2761 gagctgctgc ggtttctggg tcagaacgtg catgcccgga agaataagaa cgtcgacatt
     2821 ctctggcaag ctgctgagat ctgccgccgc cttaatgggg tccggttcac cagctgcaag
     2881 agcgctaagg accgtacagc catgtcggtg acactggagc agtgcctgat cctgcaacac
     2941 gagcatggca tggccccgca ggtcttcacc caggccctgg agtgcatgcg cagtgagggt
     3001 tgtcgaagag aaaatacaat gaagaatgtt ggaagtcgca aatatgcatt taattccctg
     3061 cagctgaagg ctttccccaa gcattacagg cctcccgaag ggacttacgg aaaagttgaa
     3121 acgtgaacac acggtttcct ctaattagct gttacataat aaatgtgggt accctctagt
     3181 gtcatatatg aattcttcaa gaagacctga aggattggtt tttatttttt gtggtttttt
     3241 taaaaaaaac atttcactaa agagtctctg gagcatgttt tttgtttttt gggttttttt
     3301 ccccattgga atcaatagga ggtaatgttt ggctcaatag tgtggatagt aacaactgcc
     3361 tatttaaatt aaatagcctt tggctgtaac tacacagatc tcatcaatag ttacctacat
     3421 gaatcaagct aggtttgtct gaaatgctag aagacttttt caaatgcctg cttgctcact
     3481 tacttttcta cagagtaact caaaagtaac cattgaatta caatccactt tgtaaaaatc
     3541 tgaatgtaaa acagtaaata ataaattaaa taattggatc ctaggcatac caataaatgt
     3601 tatttgggga aagaagctag cactatctca tgcagtgaga ataacgggtt ctagtgaatt
     3661 atcctatcta cactaccacc tgaagaagtt gaaagaaaga ttgataaatc aacgggagat
     3721 aagactttat tagaaggtaa cacctgcaag gtaccccaca tagttggggc cactcttttt
     3781 tctgtccctt atgagttaca gaacagggag tctgctggag acacaaagcc agcagtaagg
     3841 tccctttagc ctgtctcctg ttctctgaag ctccctgccg aggctccctc acgcccagag
     3901 gacacatctc cccttcttct ctagatgtta atgtagggcc tcagatgtgg catgtcaaca
     3961 ctcccatggg gaatttatga ccatagagat agtagaaacc taaatatttt cagaaaaaaa
     4021 attttttttg caggatttcc aatttatctt tgaaaagaca gtacaatcat gtttgaatgt
     4081 ctgatgaaag tgtcttattt ttaggtttgc aataattgtt actttcttat tctcattctt
     4141 atgtaagatg actattgaga aacagttgaa atttattctc ctcaaatgag attttgaaag
     4201 ggatttatgg gccataaaac ttaggaattt catagaaaat tttgtctggt catcttttat
     4261 aagatgatga tgagtcttat ctgcacatag cagagtttgt ttcttaggag tttatatgtt
     4321 ttattgattc tgtttacgat gtttacatgt tcttgaaaag gttgtaaaag aaatacatat
     4381 ggcacatatt aatgatgctg atcctaatga tttgtgaacc tcttagaaat tcttttgtct
     4441 aaaagcctag agattcttct ggttccttta gaaacatgta acaaacttgg gccaggggtg
     4501 ctgaggcatg gggaggactg cttattggca ggaacacaga gggggtgggc atcaggtagg
     4561 gtggaactgg atgaccttcc agaccccctc ctactcagaa gtcagcatgc aagcagcgcc
     4621 tgctgtctcc cgatgcctgc agggaggaaa atgtctgtga aggacacttt aatgaaggac
     4681 gaagctctct gctgctccct caaagctgag ttcactgtgg caggcaggaa gcagagaagc
     4741 tcctgcccgc caaacccact gcacaggtgg ctgctgctgg tatcagattc tcctttttaa
     4801 gtgtcaattt ctgttcacag acagtttgtt ttcaatatgg ctgatttacc ctttcacctc
     4861 tccaccatct catttaattt gtaaattttt agttctgtct tttctcattt taacttttaa
     4921 aaaatctcct tgctttttaa aaataaatat tacccacata gttttcagat cttttttgtc
     4981 tggagagcat taaatatctg cactcatcaa agggaatata gcaaatgttg atcatctgtc
     5041 agcagttttg catgtgacac ctttatatga attattttcc caatttttgc tctatgtaat
     5101 gggtcaccat aaaactgtaa gacttggcaa atgctataga aaaggttcag gtttcagtat
     5161 tctctctgaa ggcatcattc ctgggcttcc ggcagcatca cacgcttcca gtgcctgcag
     5221 ggtgagggtg tgtggcacag aggcaagcag ctcctctctt ctgtaggagc tttgggtttt
     5281 cagaggtgca gggaaggcag ttttacaggt taccgtaaaa cagaggttcc gtccagtgtt
     5341 ccttatgatg cctccccatt taaaaagaaa agagatctat ggaaaactgg gaatgtaatg
     5401 tggattctgt cagagctcct acagagcaca gttgccttta gtttccttta aagatgtaaa
     5461 aatattgtat aatacagttt tgtccctaca caattgtatt tgccaagctt agtgcattat
     5521 gataccttta tttatttgtt ttgggcagta ttactatata tatataaaca tacagttact
     5581 gttttatata ttcttaggtc attcaaagcc atgtatgctg taaatgtgct agtctttaga
     5641 atgacacata ataaataact gacaagatat taaatgtggt cttgcctgat tcatcctatt
     5701 aggaggacta gttctatgtg accagcctgt gactggacca ttgttcttgg gcattgcatt
     5761 gagtgctcgg gtgctgaata catgtttgta aacatgggac catgggagac ttccatttta
     5821 taagcttgtt tctgcctaat ttgaagcaga ggggcaacag cgttgaggtt aagagcacgg
     5881 actttgggag cacacagacc aggtcccaat cctggttctg cccctgactg gctggtgact
     5941 ctgagcaagt tgcttgacct ctccaagcct ccgtttcctc atgtgcaaag tgtggacaac
     6001 aacagtacct tcctcataag gaacgtgcga cgcgcctcag aagtacgtgt tcataaatgg
     6061 tagccattgt tgttaccttc ccgtctgtga acatggatca catcatctct gtgggtaacc
     6121 cagtcctcgt tgtatgactt gtcaaaatgc agttccactt gtattaatat tgaccctgtt
     6181 catcgtcacc aggactgcat cttgcaccac tgtgccgtct tctaggcaca cacgacccgt
     6241 tatctccctt ggaaaaattc ctctcactgg aaatgtacaa ttaaaggatg attgaaatgt
     6301 ttaactcttg gagtttttag tattaccaaa ctcctagaaa tttaagctac caccaaaaat
     6361 aacatgtgcc ttttcccttt attctcattg atggtagcag tgcacctcgt ttcagtgtcc
     6421 tgattccttg attactgtga acactgaaca tcttttatac ctttatgata tttctatgtt
     6481 tgtgacttca ttggaatgtt gatctatttc atatagattg tttttaatat acaatgtatt
     6541 agcccctgtc atatgttgca cactttttaa cctatttgtc agctgtcttt tggtctgtgg
     6601 taggagttat acagtatttt aaatggttta tgtagtcaca tatacctgtc tttcattttg
     6661 atgcttgttc tgaaatggag tcctggcttc accatggaag gtgggacata tgggacagcc
     6721 atgtccttgt gggcatggtg gtgtggctgc gtgcccacga cgaggggctg gactaaagtg
     6781 ctcactggca ccttgcttgg tgcatgggcc atgctcagtt taatattatt aacagtttct
     6841 accttcactc tcatgtctca ggattatttt ttcagcattt acctgttctt atttctagga
     6901 tgtttatggt ttcagtccta catttagatc aatccatctg gaatcatctt ggtgtaagtc
     6961 ctgtgttgtg cttacagtat gtaaagtcag agggaatccc tcaaggtcag gcctaatttt
     7021 gcttctcttt aaagttaagc caagaagaag acatgtcagg acgcccagac atcagctacc
     7081 gtgagcttct tcctgccctc cctccgcatg ccattctggt gctgcgcttc agtttcctgt
     7141 tgagtctctg cttagtagat gacttggcac aatctggctg cccagatcta ggggagttgt
     7201 cccagggagg ctttgcgctg gaggaaggta agaggagctg gaggttggag cttcccgatt
     7261 tgtgattttt ccaagaacag aaatgattag ccatgtgggg aggttacttc attgtacatt
     7321 gttctactgc ataacctccc tctattcttt accagcatca cacagcaggt tggcaccatt
     7381 tttgaataga ggtttctcac agtatcctca gcagcattga gtgttcctaa tgttgtagta
     7441 atgagcagta gatgtctctc agtgggcagt gtgacctctt tggtcactct aaagggctgc
     7501 cagcatttct cttaagctgg ggggagaaga gctgttctgt gtataggggt attgggaaca
     7561 catggacaga aggaactggc ctcacatgcc atcgtgttcc tgggatggct cttccaagac
     7621 cagcagttcc tcctggcagg caggccctca cagaactctg catttggaga tggacaaact
     7681 ctcttcttct tatcactttc tccccaaaat gtcctcttct accagcccct cttaagagcc
     7741 gattttctgt aagttatagt gagggcactg ctctgctgtg cacagaagga accccagccc
     7801 agaagcacac gctccctgtg gaacctgcag gggtggcagt gatgacagaa ccaagggggt
     7861 caaggaagat tggagaggag tgggcagcag gaaacagctc aggctgcctg acctggcccc
     7921 cggctgccca tgagggcctc cagagagggg cagagctggc ttatcgggat ccatgatggt
     7981 ttttacactt ttaaaagtgc cctcttctca gagaccctgg ttatttccaa aagtaggaag
     8041 taatcaaatt tacagtggat aaaatccagt atttgaaatc aggaagacag aaaaaaaagc
     8101 aaaatagcat ccttttctga gaggtatatg ctgaccaaaa agatctttca ttcctgtgaa
     8161 caccaagcag cgcggcctct ggcagagaga gcgctgggtt gtacagatgg aaacttgtgt
     8221 ttagcaggct gtctgctccc aatagtggcc ctggctgcaa ctgacctcct gatgccctgc
     8281 tgacgctaac tgtgggtacc aaccagcacc gtggacatcg gccccaggat cagtctacga
     8341 tttgtctggt ggccaaatga tgacttttga gtgggaaaag ctggtggggg ctacaccgtg
     8401 ctctgcagtg tagtgggatc ctgtgagctg gcctgcacag gcactccctc ccctgccagg
     8461 ggccccaggg aggcttctcc agcctgaggc tgcaccctcc atacgattcc accccccatc
     8521 ctgtgccaac agtaggcaaa cggagagtag cttggtcttg tttttattag ctgttgccct
     8581 ccaggctacg acagggttcc cagggtgtag cctgaaaaca cccatactct agattccctc
     8641 cactggaacc ctgtctttgc catgatttct tcctgcatca ggcccctgtg ggccttgcag
     8701 gccagtccag gcaggtcttt cacactgttg tcccacataa cagaaaaagc tgagcagaca
     8761 gggtaggaaa cacacttgca tctccatggc tccaaagagg atgcctgtgt tagatggatg
     8821 tagacagaca cagcacttca ctgcttcctc ctcctcgcca cccagccctc cctcggtgtc
     8881 tgtggcctga ctctctcctg gtggtcctac gtctctgacc atccctcctc actctcaaat
     8941 tttatgttgc ccaccacctg ccactaatgg cgtggtgttc tcctgggttc cctttctcac
     9001 ttaagatgat cttcctgaaa aatcttacac attcctgttg aacaaatggt cacttatatg
     9061 gtagtgactc ctaaatttct aggtccatcc cagactctcc cccaaggtcc agattaccta
     9121 tacatctaag caccatgcct gtcttgcagg catctcaaat acagcatgct catgcctctc
     9181 ctcctccaag ctggctgagt ctccctctaa tagacgacct tgggatcatt ttctcctcca
     9241 catcccatca atcagccact gaacctgtat ctcttgagaa ccatcccata atttccatcc
     9301 atgcacccca aggcctggtc tgtatcagtc ccctcggatc tggacctctc ctgagcatcc
     9361 tcacccctcc tgcaaggcta gtctcaggtc tgccttcctc tatggtgatt tctccccaca
     9421 aatgaagttg gccctttcaa aaaaacttcc tctccttcat tgttgactat gaagattctt
     9481 tcatgcatgt cctgtccact gtcagtcccc tctgtattca caagagcaag tgtaatgccc
     9541 gctgtgaagg agctaaagtt tgttgaataa acacatgaac aaatggtggt tttgtggtgt
     9601 tcaactgaaa tatttcctat ttcagcactg gaccacgctg tttttctcac tacataatcg
     9661 gttacgctaa atgcatcacg agccaaattc aaaaggtcaa aggagaaggc aaatggcaga
     9721 gtgtggtaaa ggggcgagtg agagggtctc tgaaaaaagt caggctaaaa ttacaggggg
     9781 ttcaggcaat gttatcttga tctggatggc cagttacatg acagattact ttgttcttaa
     9841 catacgaaca tcctattcac tttttggtat actggttgtt taacagtaaa aattaaatgt
     9901 ttaaaaccca
//
