LOCUS       NM_022489               7623 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens inverted formin 2 (INF2), transcript variant 1, mRNA.
ACCESSION   NM_022489
VERSION     NM_022489.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Palmer,N.J., Barrie,K.R. and Dominguez,R.
  TITLE     Mechanisms of actin filament severing and elongation by formins
  JOURNAL   Nature 632 (8024), 437-442 (2024)
   PUBMED   38843827
  REMARK    GeneRIF: Mechanisms of actin filament severing and elongation by
            formins.
REFERENCE   2  (bases 1 to 7623)
  AUTHORS   Labat-de-Hoz,L., Fernandez-Martin,L., Correas,I. and Alonso,M.A.
  TITLE     INF2 formin variants linked to human inherited kidney disease
            reprogram the transcriptome, causing mitotic chaos and cell death
  JOURNAL   Cell Mol Life Sci 81 (1), 279 (2024)
   PUBMED   38916773
  REMARK    GeneRIF: INF2 formin variants linked to human inherited kidney
            disease reprogram the transcriptome, causing mitotic chaos and cell
            death.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7623)
  AUTHORS   Wei,J., Wen,Q., Zhan,S., Cao,J., Jiang,Y., Lian,J., Mai,Y., Qiu,M.,
            Liu,Y., Chen,P., Lin,Q., Wei,X., Wei,Y., Huang,Q., Zhang,R., He,S.,
            Yuan,G., Wei,Q., Zhou,Z. and Yu,H.
  TITLE     Functional genetic variants of the disulfidptosis-related INF2 gene
            predict survival of hepatitis B virus-related hepatocellular
            carcinoma
  JOURNAL   Carcinogenesis 45 (4), 199-209 (2024)
   PUBMED   38270181
  REMARK    GeneRIF: Functional genetic variants of the disulfidptosis-related
            INF2 gene predict survival of hepatitis B virus-related
            hepatocellular carcinoma.
REFERENCE   4  (bases 1 to 7623)
  AUTHORS   Ding,Y., Lv,Z., Cao,W., Shi,W., He,Q. and Gao,K.
  TITLE     Phosphorylation of INF2 by AMPK promotes mitochondrial fission and
            oncogenic function in endometrial cancer
  JOURNAL   Cell Death Dis 15 (1), 65 (2024)
   PUBMED   38233384
  REMARK    GeneRIF: Phosphorylation of INF2 by AMPK promotes mitochondrial
            fission and oncogenic function in endometrial cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7623)
  AUTHORS   Zhang,H., Zhao,Y., Wang,J., Li,J., Xia,J., Lin,Y., Zhong,Y.,
            Cao,X., Jin,J., Li,X., Yang,W., Ye,M. and Jin,X.
  TITLE     FBXO7, a tumor suppressor in endometrial carcinoma, suppresses
            INF2-associated mitochondrial division
  JOURNAL   Cell Death Dis 14 (6), 368 (2023)
   PUBMED   37344480
  REMARK    GeneRIF: FBXO7, a tumor suppressor in endometrial carcinoma,
            suppresses INF2-associated mitochondrial division.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7623)
  AUTHORS   Boyer,O., Benoit,G., Gribouval,O., Nevo,F., Tete,M.J., Dantal,J.,
            Gilbert-Dussardier,B., Touchard,G., Karras,A., Presne,C.,
            Grunfeld,J.P., Legendre,C., Joly,D., Rieu,P., Mohsin,N.,
            Hannedouche,T., Moal,V., Gubler,M.C., Broutin,I., Mollet,G. and
            Antignac,C.
  TITLE     Mutations in INF2 are a major cause of autosomal dominant focal
            segmental glomerulosclerosis
  JOURNAL   J Am Soc Nephrol 22 (2), 239-245 (2011)
   PUBMED   21258034
  REMARK    GeneRIF: Six of the seven distinct altered residues localized to an
            INF2 region that corresponded to a subdomain of the mDia1
            diaphanous inhibitory domain reported to co-immunoprecipitate with
            IQ motif-containing GTPase-activating protein 1
REFERENCE   7  (bases 1 to 7623)
  AUTHORS   Lee,H.K., Han,K.H., Jung,Y.H., Kang,H.G., Moon,K.C., Ha,I.S.,
            Choi,Y. and Cheong,H.I.
  TITLE     Variable renal phenotype in a family with an INF2 mutation
  JOURNAL   Pediatr Nephrol 26 (1), 73-76 (2011)
   PUBMED   20803156
  REMARK    GeneRIF: In conclusion, we described an additional familial case of
            the autosomal dominant form of focalsegmental glomerulosclerosis
            associated with INF2 mutations.
REFERENCE   8  (bases 1 to 7623)
  AUTHORS   Brown,E.J., Schlondorff,J.S., Becker,D.J., Tsukaguchi,H.,
            Tonna,S.J., Uscinski,A.L., Higgs,H.N., Henderson,J.M. and
            Pollak,M.R.
  TITLE     Mutations in the formin gene INF2 cause focal segmental
            glomerulosclerosis
  JOURNAL   Nat Genet 42 (1), 72-76 (2010)
   PUBMED   20023659
  REMARK    GeneRIF: Study identified nine independent nonconservative missense
            mutations in INF2, which encodes a member of the formin family of
            actin-regulating proteins.
            Erratum:[Nat Genet. 2010 Apr;42(4):361. Tonna, Stephen J [added]]
REFERENCE   9  (bases 1 to 7623)
  AUTHORS   Chhabra,E.S. and Higgs,H.N.
  TITLE     INF2 Is a WASP homology 2 motif-containing formin that severs actin
            filaments and accelerates both polymerization and depolymerization
  JOURNAL   J Biol Chem 281 (36), 26754-26767 (2006)
   PUBMED   16818491
REFERENCE   10 (bases 1 to 7623)
  AUTHORS   Bindschadler,M. and McGrath,J.L.
  TITLE     Formin' new ideas about actin filament generation
  JOURNAL   Proc Natl Acad Sci U S A 101 (41), 14685-14686 (2004)
   PUBMED   15466701
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI489810.1, BX248757.1,
            BQ961756.1, AL583722.6, DQ395340.1, DA500952.1, BU538960.1,
            CD631414.1, DQ395339.1 and BG820892.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_022489.3.
            
            Summary: This gene represents a member of the formin family of
            proteins. It is considered a diaphanous formin due to the presence
            of a diaphanous inhibitory domain located at the N-terminus of the
            encoded protein. Studies of a similar mouse protein indicate that
            the protein encoded by this locus may function in polymerization
            and depolymerization of actin filaments. Mutations at this locus
            have been associated with focal segmental glomerulosclerosis
            5.[provided by RefSeq, Aug 2010].
            
            Transcript Variant: This variant (1) encodes isoform 1. Variants 1,
            5, and 6 all encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.2105929.1,
                                           SRR7346977.1714063.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392634.9/ ENSP00000376410.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                BI489810.1         13-31
            20-970              BX248757.1         1-951
            971-1367            BQ961756.1         269-665
            1368-1727           AL583722.6         114454-114813
            1728-2770           DQ395340.1         1-1043
            2771-2879           DA500952.1         444-552
            2880-3196           DQ395340.1         1153-1469
            3197-3235           BU538960.1         685-723
            3236-3337           DQ395340.1         1509-1610
            3338-3342           CD631414.1         346-350
            3343-4693           DQ395339.1         1616-2966
            4694-4698           BG820892.1         305-309
            4699-7623           AL583722.6         126561-129485
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.33"
     gene            1..7623
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /note="inverted formin 2"
                     /db_xref="GeneID:64423"
                     /db_xref="HGNC:HGNC:23791"
                     /db_xref="MIM:610982"
     exon            1..122
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            123..522
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..3881
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /note="isoform 1 is encoded by transcript variant 1;
                     HBEAG-binding protein 2 binding protein C; HBEBP2-binding
                     protein C; inverted formin, FH2 and WH2 domain containing"
                     /codon_start=1
                     /product="inverted formin-2 isoform 1"
                     /protein_id="NP_071934.3"
                     /db_xref="CCDS:CCDS9989.2"
                     /db_xref="GeneID:64423"
                     /db_xref="HGNC:HGNC:23791"
                     /db_xref="MIM:610982"
                     /translation="MSVKEGAQRKWAALKEKLGPQDSDPTEANLESADPELCIRLLQM
                     PSVVNYSGLRKRLEGSDGGWMVQFLEQSGLDLLLEALARLSGRGVARISDALLQLTCV
                     SCVRAVMNSRQGIEYILSNQGYVRQLSQALDTSNVMVKKQVFELLAALCIYSPEGHVL
                     TLDALDHYKTVCSQQYRFSIVMNELSGSDNVPYVVTLLSVINAVILGPEDLRARTQLR
                     NEFIGLQLLDVLARLRDLEDADLLIQLEAFEEAKAEDEEELLRVSGGVDMSSHQEVFA
                     SLFHKVSCSPVSAQLLSVLQGLLHLEPTLRSSQLLWEALESLVNRAVLLASDAQECTL
                     EEVVERLLSVKGRPRPSPLVKAHKSVQANLDQSQRGSSPQNTTTPKPSVEGQQPAAAA
                     ACEPVDHAQSESILKVSQPRALEQQASTPPPPPPPPLLPGSSAEPPPPPPPPPLPSVG
                     AKALPTAPPPPPLPGLGAMAPPAPPLPPPLPGSCEFLPPPPPPLPGLGCPPPPPPLLP
                     GMGWGPPPPPPPLLPCTCSPPVAGGMEEVIVAQVDHGLGSAWVPSHRRVNPPTLRMKK
                     LNWQKLPSNVAREHNSMWASLSSPDAEAVEPDFSSIERLFSFPAAKPKEPTMVAPRAR
                     KEPKEITFLDAKKSLNLNIFLKQFKCSNEEVAAMIRAGDTTKFDVEVLKQLLKLLPEK
                     HEIENLRAFTEERAKLASADHFYLLLLAIPCYQLRIECMLLCEGAAAVLDMVRPKAQL
                     VLAACESLLTSRQLPIFCQLILRIGNFLNYGSHTGDADGFKISTLLKLTETKSQQNRV
                     TLLHHVLEEAEKSHPDLLQLPRDLEQPSQAAGINLEIIRSEASSNLKKLLETERKVSA
                     SVAEVQEQYTERLQASISAFRALDELFEAIEQKQRELADYLCEDAQQLSLEDTFSTMK
                     AFRDLFLRALKENKDRKEQAAKAERRKQQLAEEEARRPRGEDGKPVRKGPGKQEEVCV
                     IDALLADIRKGFQLRKTARGRGDTDGGSKAASMDPPRATEPVATSNPAGDPVGSTRCP
                     ASEPGLDATTASESRGWDLVDAVTPGPQPTLEQLEEGGPRPLERRSSWYVDASDVLTT
                     EDPQCPQPLEGAWPVTLGDAQALKPLKFSSNQPPAAGSSRQDAKDPTSLLGVLQAEAD
                     STSEGLEDAVHSRGARPPAAGPGGDEDEDEEDTAPESALDTSLDKSFSEDAVTDSSGS
                     GTLPRARGRASKGTGKRRKKRPSRSQEEVPPDSDDNKTKKLCVIQ"
     exon            523..638
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            639..798
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            799..832
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            833..974
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1116
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1866
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            1867..2018
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2080
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2183
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            2184..2269
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            2270..2370
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            2371..2441
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2549
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            2550..2620
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..2741
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            2742..2906
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            2907..3009
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            3010..3171
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            3172..3825
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            3826..3882
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     exon            3883..7623
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4676..4681
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /note="hexamer: AATAAA"
     polyA_site      4693
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /note="major polyA site"
     regulatory      7604..7609
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
                     /note="hexamer: AACAAA"
     polyA_site      7623
                     /gene="INF2"
                     /gene_synonym="C14orf151; C14orf173; CMTDIE; FSGS5;
                     pp9484"
ORIGIN      
        1 gccaggagcc accgtccgag ccttgcggag cgcggcagtg ggcgccggct gcccgcagcc
       61 cctgacccgg ccccggacgg agcgccggcc gcaccaccgc cctctggccg ttgcctcacc
      121 ggctcggcaa gatgtcggtg aaggagggcg cacagcgcaa gtgggcagcg ctgaaggaga
      181 agctggggcc acaggattcg gaccccacgg aggccaacct ggagagcgcg gaccccgagc
      241 tgtgcatccg gctgctccag atgccctctg tggtcaacta ctccggcctg cgcaagcgcc
      301 tggagggcag cgacggcggc tggatggtgc agttcctgga gcagagcggc ctggacctgc
      361 tgctggaggc gctggcgcgg ctgtcgggcc gcggcgttgc acgtatctcc gacgccctgc
      421 tgcagctcac ctgcgtcagc tgcgtgcgcg ccgtcatgaa ctcgcggcag ggcatcgagt
      481 acatcctcag caaccagggc tacgtgcgcc agctctccca ggccctggac acatccaacg
      541 tgatggtgaa gaagcaggtg tttgagctac tggctgccct gtgcatctac tctcccgagg
      601 gccacgtgct gaccctggac gccctggacc actacaagac ggtgtgcagc cagcagtacc
      661 gcttcagcat tgtcatgaac gagctctccg gcagcgacaa cgtgccctac gtggtcaccc
      721 tgcttagcgt gatcaacgcc gtcatcttgg gccccgagga cctgcgcgcg cgcacccagc
      781 tgcggaacga gtttatcggg ctgcagctgc tggacgtcct ggctcgcctg cgagacctgg
      841 aggatgccga cctgctgatc cagctggagg ctttcgagga ggctaaggcc gaggacgagg
      901 aggagctgct gcgagtctct ggcggggtcg acatgagcag ccaccaggag gtctttgcct
      961 ccctgttcca caaggtgagc tgctccccgg tgtctgccca gctcctgtcg gtgctgcagg
     1021 gcctcctgca cctggagccc accctccgct ccagccagct gctctgggag gccctggaga
     1081 gcctcgtgaa ccgggccgtg ctcctggcca gcgatgccca ggaatgcacc ctggaggaag
     1141 tggttgagcg gctcctgtct gtcaaggggc gacccagacc gagccccctg gtcaaggccc
     1201 ataaaagcgt ccaggccaac ctagaccaga gccagagggg cagctccccg caaaacacta
     1261 caacccccaa gcccagcgtg gagggccagc agccagcagc agctgctgcc tgcgagcccg
     1321 tggaccacgc ccagagtgag agcatcctga aagtttcgca gcccagagcc ctggagcagc
     1381 aggcgtccac cccaccccca cccccacccc cacccctgct ccctggttcc agtgccgagc
     1441 cccctccccc tcccccacca ccccccctgc ccagtgtggg ggctaaggcc ctcccaacag
     1501 cacccccgcc cccacccctg ccaggcctgg gggccatggc ccccccagca cctcctctac
     1561 caccacccct gccaggctcc tgtgagttcc tgcccccacc acctccacca ctcccgggct
     1621 tgggatgccc gcccccaccc ccacccctgc tgcctggtat gggctggggc cctcctccac
     1681 ccccacctcc actactgccc tgcacctgca gcccccccgt ggcgggaggc atggaggagg
     1741 tcatcgtggc ccaggtggac catggcttgg gctcagcatg ggtccccagc catcggcggg
     1801 tgaacccacc cacactgcgc atgaagaagc tgaactggca gaagctgcca tccaacgtgg
     1861 cacgtgagca caactctatg tgggcgtccc tgagcagccc cgacgccgag gctgtggagc
     1921 ccgacttctc cagcatcgag cgactattct ccttccctgc agccaagccc aaggagccca
     1981 ccatggtggc cccccgggcc aggaaggagc ccaaggagat cactttcctc gatgccaaga
     2041 agagcctgaa cctcaacatc ttcctgaagc aatttaagtg ctccaacgag gaggtcgctg
     2101 ctatgatccg ggctggagat accaccaagt ttgatgtgga ggttctcaaa caactcctta
     2161 agctccttcc cgagaagcac gagattgaaa acctgcgggc attcacagag gagcgagcca
     2221 agctggccag cgccgaccac ttctacctcc tcctgctggc cattccctgc taccagctgc
     2281 gaatcgagtg catgctgctg tgtgagggcg cggccgccgt gctggacatg gtgcggccca
     2341 aggcccagct ggtgctggct gcctgcgaaa gcctgctcac cagccgccag ctgcccatct
     2401 tctgccagct gatcctgaga attgggaact tcctcaacta cggcagccac accggtgacg
     2461 ccgacggctt caagatcagc acattgctga agctcacgga gaccaagtcc cagcagaacc
     2521 gcgtgacgct gctgcaccac gtgctggagg aagcggaaaa gagccacccc gacctcctgc
     2581 agctgccccg ggacctggaa cagccctcgc aagcagcagg gatcaacctg gagatcatcc
     2641 gctcagaggc cagctccaac ctgaagaagc ttctggagac cgagcggaag gtgtctgcct
     2701 ccgtggccga ggtccaggag cagtacaccg agcgcctcca ggccagcatc tcggccttcc
     2761 gggcactgga tgagctgttt gaggccatcg agcagaagca acgggagctg gccgactacc
     2821 tgtgtgagga cgcccagcag ctgtccctgg aggacacgtt cagcaccatg aaggctttcc
     2881 gggacctttt cctccgcgcc ctgaaggaga acaaggaccg gaaggagcag gcggcgaagg
     2941 cagagaggag gaagcagcag ctggcggagg aggaggcgcg gcggcctcgg ggagaggacg
     3001 ggaagcctgt caggaagggg cccgggaagc aggaggaggt gtgtgtcatc gatgccctgc
     3061 tggctgacat caggaagggc ttccagctgc ggaagacagc ccggggccgc ggggacaccg
     3121 acgggggcag caaggcagcc tccatggatc ccccaagagc cacagagcct gtggccacca
     3181 gtaaccctgc aggagatccc gtgggcagca cgcgctgtcc cgcctctgag cccggccttg
     3241 atgctacaac agccagcgag tcccggggct gggaccttgt agacgccgtg acccccggcc
     3301 ctcagcccac cctggagcag ttggaggagg gtggtccacg gcccctggag aggcgttctt
     3361 cctggtatgt ggatgccagc gatgtcctaa ccactgagga tccccagtgc ccccagccct
     3421 tggagggggc ctggccggtg actctgggag atgctcaggc cctgaagccc ctcaagttct
     3481 ccagcaacca gccccctgca gccggaagtt caaggcaaga tgccaaggat cccacgtcct
     3541 tgctgggcgt cctccaggcc gaggccgaca gcacaagtga ggggctggag gacgctgtcc
     3601 acagccgtgg tgccagaccc cctgcagcag gcccaggtgg ggatgaggac gaggacgagg
     3661 aggacacggc cccagagtcc gcactggaca catccctgga caagtccttc tccgaggatg
     3721 cggtgaccga ctcctcgggg tcgggcacac tccccagggc ccggggccgg gcctcaaagg
     3781 ggaccgggaa gcgaaggaag aagcgtccct ccaggagcca ggaagaggtt ccccctgatt
     3841 ctgatgataa taaaacaaag aaactgtgtg tgatccagta aggcctcagg cccaggccca
     3901 aggccaagtg agagagccca ggccacagga catgctgcca ttctgccaag agaggctctt
     3961 ctgggggcca ggctgggact gggccccgga aaccaaaact ccgtgcctta cccagccggg
     4021 gccctcctgg agccttcttg gggtgttgtg gctgggaacc cgacaggcac cagtgccctg
     4081 ccaggcctgg tgccctcctg gaccgcctgc acgtgccagc ctcccacctg cttcctaaag
     4141 gcaaccctgg cccacacccg catgcgcccg gtgcagcctg ccaagggcca gtcggggggt
     4201 gctgcgtcct gccagtgtcc accacagctc tgcctgccct tcagcccagc aaggtttaat
     4261 caaaatgcaa tgctttgcaa gtctttactg cttggaggtg gctgagttgg gggccctggg
     4321 caggggtaag ctggcaggca gtgccatggc aggccagggt cccctcccat ggggtctggc
     4381 ccccgttcca gcatgtccag cccctgaagt tggagtgggg ggcggtctgc ctttgctgcc
     4441 actgccaggc ctctgccctg cagctgaaac ttggccatca catcaacaga aaacccctcc
     4501 cagtgccagc tgcccagcgt gggcaggccc tggggacaat acaggtccac ctgaggggct
     4561 gcagggtgac acccagcagc cgctgccccc tcactgccca cccagcgagg gcagcctacc
     4621 cgagcctgcc ccctgccagg tgtgtgccct gaggctggcg gctggatgcg tggccaataa
     4681 aaagcagacc tagcccggtg tgcgactgtc gtgttcagag gctgcgggag tgggcggtgg
     4741 acgtgggctc ccacaaacat ggtgagcgct ggacaggccc cgtgtgaaca ctcactgttg
     4801 aggggccccc aggccccgtg tgaacactca ctgttgaggg gtccctgggc ccccaggcac
     4861 cgtgtgagca ttcactgttg agggtccccc agtcccccag gccaggggct gtccctggag
     4921 ccctcaggag gcagtaagaa acatctagaa gagggcagac ccagtggtga accgcatcca
     4981 acagtggtga gatgggtgca gctctacagg ggcgtaattt acaaaccacc agactccctg
     5041 gttatgtgtg tgctgcggtg gtgtttgctc tccagcagat caagtttgtg caatgatcca
     5101 ttttgagaat ctctccgtca ccccaagaag tcctctcgca cccatcagtc cgaccccttc
     5161 cccgtgctcc tccccagccc tggcagccac tcacctgcac tccagacgct ccagatgtca
     5221 tgcatgtctc ctggcttcct tcggctgttt tcacgcgcca tcctcacggc aggctgtatc
     5281 aggacttcat tccatttcat ggctgaagca cattccattg catgcaggga ccacattttg
     5341 tttgtctgct cttctgtcca tggacatctg ggttgcttcc agtttggggc ttctgtgaat
     5401 cctgctgctg tggacatttg cgtagtcctc gtgtggatgc tgctgtggac gtctgcgtcg
     5461 tcctcgtgtg gatgctgctg tggacgtctg cgtcgtcctc gtgtggatgc tgctgtggac
     5521 gtctgcgtag tctcgtgtgg atgctgctgt ggacgtctgc gtcgtcctcg tgtggatgct
     5581 gctgtggaca tctgcgtagt ctcgtgtgga tgctgctgtg aacgtctgcg tagtctcgtg
     5641 tggatgctgc tgtggacgtc tgcgtagtct cgtgtggatg ctgctgtgca cgtctgcgtc
     5701 gtcctcgtgt ggatgctgct gtggacgtct gcgtcgtcct cgtgtggatg ctgctgtgga
     5761 cgtctgcgtc gtcctcgtgt ggatgctgct gtggacgtct gcgtcgtctc gtgtggatgc
     5821 tgctgtggac gtctgcgtcg tctcgtgtgg atgctgctgt ggacgtctgc gtcgtctcgt
     5881 gtggatgctg ctgtggacgt ctgcgtcgtc ctcgtgtgga tgctgctgtg gacgtctgcg
     5941 tcgtctcgtg tggatgctgc tgtggacgtc tgcgtcgtcc tcgtgtggat gctgctgtgg
     6001 acgtctgcgt cgtcctcgtg tggatgctgc tgtggacgtc tgcgtcgtcc tcgtgtggat
     6061 gctgctgtgg acgtctgcgt cgtcctcctg tggatgctgc tgtggacgtc tgcgtcgtcc
     6121 tcgtgtggat gctgctgtgg acgtctgcgt agtctcgtgt ggatgctgct gtggacgtct
     6181 gcgtagtctc gtgtggatgc tgctgtggac gtctgcgtcg tcctcgatgc tgctgtggac
     6241 gtctgcgtcg tcctcgtgtg gatgctgcta tggacgtctg cgtagtctcg tgtggatgct
     6301 gctgtggacg tctgcgtcgt cctcgtgtgg atgctgctgt ggacgtctgc gtagtctcgt
     6361 gtggatgctg ctgtggacgt ctgcgtagtc tcgtgtggat gctgctgtgg acgtctgcgt
     6421 cgtcctcgtg tggatgctgc tgtggacgtc tgcgtagtct cgtgtggatg ctgctgtgga
     6481 cgtctgcgtc gtcctcgtgt ggatgctgct gtggacgtct gtgtagtctc atgtggatgc
     6541 tgcgagtttt cgttgctctg gggtagattc gtagcggagg aacaactggg cgacacgcta
     6601 agtttatgtt taacttttta agtaactgcc taactgtttt ccaaagcagc tgcaccattt
     6661 ttcattccca tccatgctat tgtttttcag ttaaatagct ccactcctcc ccctccccac
     6721 cctccacaga actgccagta aaactggact atggagtatt gggtggggtc acctacaagg
     6781 tcatctgcag caagtgagac catcgcccct cctgcctgga gaggtgggac tggggaccct
     6841 gtgacaggag gagctgccct tcctggagcc tggtttttct ttttcctctg tgctccatga
     6901 gaagtcagtt tagtgggcta cagccagcaa aggaaccaaa accaacagga gctctggggg
     6961 tgccttgcat ggcgaggctc cgtggctgca cgcgtgtatg caggtggctg tgggtgtctg
     7021 tgtgtcctgg gccactgtgt gcgatggtcc cactgggagg tgtggccccc aaagagtgga
     7081 acccacaggt ggacacaccc atgcaccctc gttcttgccc aaggtcacac atcgagacag
     7141 cagggcccag atggatggcc ccaagctcgg tggctcccgt gctgcccaca cctggggaca
     7201 ggcccactgt ggggggtaag gggtggaggt gttctaattc gggctgagcc gtgtgactgg
     7261 caggggcccc agctgttggg cctgggcagg actgttttta gaaaagcccc aactccctgc
     7321 ccctacccgg gcccgtggct atttatagtc cgggcctgga gaagtcagtg agtcactagc
     7381 gttcgcctct gcagggtcag tccaagcccc cttagtgagg tggtctagca tgtcccccag
     7441 tcccccaagc ctctatctga catctgctag cccagagccc caggggtccc caggaagggc
     7501 tcagagctgg tggccccccc caggccttgg cagcacctcc ttgtctgatg ggactttgtg
     7561 gttcctggtg gacatttaac attgtcggtt tctctcatct ggaaacaaac ctccatgacg
     7621 ctc
//
