LOCUS       NM_032727               3251 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens internexin neuronal intermediate filament protein
            alpha (INA), mRNA.
ACCESSION   NM_032727 NM_004692
VERSION     NM_032727.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Hsu,Y.H., Pintacuda,G., Liu,R., Nacu,E., Kim,A., Tsafou,K.,
            Petrossian,N., Crotty,W., Suh,J.M., Riseman,J., Martin,J.M.,
            Biagini,J.C., Mena,D., Ching,J.K.T., Malolepsza,E., Li,T.,
            Singh,T., Ge,T., Egri,S.B., Tanenbaum,B., Stanclift,C.R.,
            Apffel,A.M., Carr,S.A., Schenone,M., Jaffe,J., Fornelos,N.,
            Huang,H., Eggan,K.C. and Lage,K.
  CONSRTM   Schizophrenia Working Group of the Psychiatric Genomics Consortium;
            Stanley Global Asia Initiatives
  TITLE     Using brain cell-type-specific protein interactomes to interpret
            neurodevelopmental genetic signals in schizophrenia
  JOURNAL   iScience 26 (5), 106701 (2023)
   PUBMED   37207277
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 3251)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3251)
  AUTHORS   Li,Y., Bai,L., Yu,H., Cai,D., Wang,X., Huang,B., Peng,S., Huang,M.,
            Cao,G., Kaz,A.M., Grady,W.M., Wang,J. and Luo,Y.
  TITLE     Epigenetic Inactivation of alpha-Internexin Accelerates Microtubule
            Polymerization in Colorectal Cancer
  JOURNAL   Cancer Res 80 (23), 5203-5215 (2020)
   PUBMED   33051252
  REMARK    GeneRIF: Epigenetic Inactivation of alpha-Internexin Accelerates
            Microtubule Polymerization in Colorectal Cancer.
REFERENCE   5  (bases 1 to 3251)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 3251)
  AUTHORS   Husi,H., Ward,M.A., Choudhary,J.S., Blackstock,W.P. and Grant,S.G.
  TITLE     Proteomic analysis of NMDA receptor-adhesion protein signaling
            complexes
  JOURNAL   Nat Neurosci 3 (7), 661-669 (2000)
   PUBMED   10862698
REFERENCE   7  (bases 1 to 3251)
  AUTHORS   Reddy,T.R., Li,X., Jones,Y., Ellisman,M.H., Ching,G.Y., Liem,R.K.
            and Wong-Staal,F.
  TITLE     Specific interaction of HTLV tax protein and a human type IV
            neuronal intermediate filament protein
  JOURNAL   Proc Natl Acad Sci U S A 95 (2), 702-707 (1998)
   PUBMED   9435256
REFERENCE   8  (bases 1 to 3251)
  AUTHORS   Chan,S.O. and Chiu,F.C.
  TITLE     Cloning and developmental expression of human 66 kd neurofilament
            protein
  JOURNAL   Brain Res Mol Brain Res 29 (1), 177-184 (1995)
   PUBMED   7769995
REFERENCE   9  (bases 1 to 3251)
  AUTHORS   Yachnis,A.T., Rorke,L.B., Lee,V.M. and Trojanowski,J.Q.
  TITLE     Expression of neuronal and glial polypeptides during histogenesis
            of the human cerebellar cortex including observations on the
            dentate nucleus
  JOURNAL   J Comp Neurol 334 (3), 356-369 (1993)
   PUBMED   7690783
REFERENCE   10 (bases 1 to 3251)
  AUTHORS   Kaplan,M.P., Chin,S.S., Fliegner,K.H. and Liem,R.K.
  TITLE     Alpha-internexin, a novel neuronal intermediate filament protein,
            precedes the low molecular weight neurofilament protein (NF-L) in
            the developing rat brain
  JOURNAL   J Neurosci 10 (8), 2735-2748 (1990)
   PUBMED   2201753
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL591408.19 and BC006359.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_032727.3.
            
            Summary: Neurofilaments are type IV intermediate filament
            heteropolymers composed of light, medium, and heavy chains.
            Neurofilaments comprise the axoskeleton and they functionally
            maintain the neuronal caliber. They may also play a role in
            intracellular transport to axons and dendrites. This gene is a
            member of the intermediate filament family and is involved in the
            morphogenesis of neurons. [provided by RefSeq, Jun 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006359.2, S78296.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369849.9/ ENSP00000358865.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AL591408.19        29067-29091
            26-2401             BC006359.2         1-2376
            2402-3251           BC006359.2         2378-3227
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.33"
     gene            1..3251
                     /gene="INA"
                     /gene_synonym="NEF5; NF-66; NF66; TXBP-1"
                     /note="internexin neuronal intermediate filament protein
                     alpha"
                     /db_xref="GeneID:9118"
                     /db_xref="HGNC:HGNC:6057"
                     /db_xref="MIM:605338"
     exon            1..1139
                     /gene="INA"
                     /gene_synonym="NEF5; NF-66; NF66; TXBP-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..1574
                     /gene="INA"
                     /gene_synonym="NEF5; NF-66; NF66; TXBP-1"
                     /note="neurofilament-66, tax-binding protein;
                     neurofilament 5 (66kD); alpha-Inx; 66 kDa neurofilament
                     protein"
                     /codon_start=1
                     /product="alpha-internexin"
                     /protein_id="NP_116116.1"
                     /db_xref="CCDS:CCDS7545.1"
                     /db_xref="GeneID:9118"
                     /db_xref="HGNC:HGNC:6057"
                     /db_xref="MIM:605338"
                     /translation="MSFGSEHYLCSSSSYRKVFGDGSRLSARLSGAGGAGGFRSQSLS
                     RSNVASSAACSSASSLGLGLAYRRPPASDGLDLSQAAARTNEYKIIRTNEKEQLQGLN
                     DRFAVFIEKVHQLETQNRALEAELAALRQRHAEPSRVGELFQRELRDLRAQLEEASSA
                     RSQALLERDGLAEEVQRLRARCEEESRGREGAERALKAQQRDVDGATLARLDLEKKVE
                     SLLDELAFVRQVHDEEVAELLATLQASSQAAAEVDVTVAKPDLTSALREIRAQYESLA
                     AKNLQSAEEWYKSKFANLNEQAARSTEAIRASREEIHEYRRQLQARTIEIEGLRGANE
                     SLERQILELEERHSAEVAGYQDSIGQLENDLRNTKSEMARHLREYQDLLNVKMALDIE
                     IAAYRKLLEGEETRFSTSGLSISGLNPLPNPSYLLPPRILSATTSKVSSTGLSLKKEE
                     EEEEASKVASKKTSQIGESFEEILEETVISTKKTEKSNIEETTISSQKI"
     exon            1140..1264
                     /gene="INA"
                     /gene_synonym="NEF5; NF-66; NF66; TXBP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..3251
                     /gene="INA"
                     /gene_synonym="NEF5; NF-66; NF66; TXBP-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3226..3231
                     /regulatory_class="polyA_signal_sequence"
                     /gene="INA"
                     /gene_synonym="NEF5; NF-66; NF66; TXBP-1"
                     /note="hexamer: ATTAAA"
     polyA_site      3251
                     /gene="INA"
                     /gene_synonym="NEF5; NF-66; NF66; TXBP-1"
                     /note="major polyA site"
ORIGIN      
        1 gccctgcctg ccgcacctct cctttcttct gtagctcgcg ttgaagccgc acgtccggcc
       61 ccgatcccgg caccatgagc ttcggctcgg agcactacct gtgctcctcc tcctcctacc
      121 gcaaggtgtt cggggatggc tctcgcctgt ccgcccgcct ctctggggcc ggcggcgcgg
      181 gcggcttccg ctcgcagtcg ctgtcccgca gcaatgtggc ctcctcggcc gcctgctcct
      241 cggcctcgtc gctcggcctc ggcctggcct atcgccggcc gccggcgtcc gacgggctgg
      301 acctgagcca ggcggcggcg cgcaccaacg agtacaagat catccgcacc aacgagaagg
      361 agcagctgca gggcctcaac gaccgcttcg ccgtgttcat cgagaaggtg catcagctgg
      421 agacgcagaa ccgcgcgttg gaggccgagc tggccgcgct gcgacagcgc cacgctgagc
      481 cgtcgcgcgt cggcgagctc ttccagcgcg agctgcgcga cctgcgcgcg cagctggagg
      541 aggccagctc ggctcgctcg caggccctgc tggagcgcga cgggctggcg gaggaggtgc
      601 agcggctgcg ggcgcgctgc gaggaggaga gccgcggacg cgaaggcgcc gagcgcgccc
      661 tgaaggcgca gcagcgcgac gtggacggcg ccacgctggc ccgcctggac ctggagaaga
      721 aggtggagtc gctgctggac gagctggcct tcgtacgcca ggtgcacgac gaggaggtag
      781 ccgagctgct ggccacgctg caggcgtcgt cgcaggccgc ggccgaggtg gacgtgactg
      841 tggctaaacc agacctgacc tcggctctga gggagatccg cgcccagtat gagtccctgg
      901 ccgctaagaa cctgcagtcc gcggaagaat ggtacaagtc caagtttgcc aacctgaacg
      961 agcaggcggc gcgcagcacc gaggccatcc gggccagccg cgaggagatc cacgagtatc
     1021 ggcgccagct gcaggcgcgc accatcgaga tcgagggcct gcgcggggcc aacgagtcct
     1081 tggagaggca gatcctggag ctggaggagc ggcacagtgc cgaggtagct ggctaccagg
     1141 atagcattgg gcagctggag aatgatctga ggaacaccaa gagtgagatg gcacgccacc
     1201 ttcgggaata ccaggacttg ctcaatgtca aaatggctct tgacattgag atagcagctt
     1261 acaggaaact gctggaaggc gaggagacac gttttagcac cagtgggtta agcatttcgg
     1321 ggctgaatcc acttcccaat ccaagttacc tgctcccacc tagaatcctc agtgctacaa
     1381 cctccaaagt ctcatccact gggctatcac ttaagaaaga ggaggaggag gaggaggcat
     1441 ctaaggtagc ctctaagaaa acctcccaga taggggaaag ttttgaagaa atattagagg
     1501 agacagtaat atctactaag aaaaccgaga aatcaaatat agaagaaacc accatttcaa
     1561 gccaaaaaat ataattccat tgctttgaaa aagttaatgc ttaagaggga atgatatgca
     1621 tttgacttgt taaacagcct attcctgaac tataacacct gccaccacta taaaatgtct
     1681 tcaaggcttc agtctcatat ttagtattga atacttacat tctctaataa gaaaaccacc
     1741 ccttagattg aagtaaactg cagtcctgga gcaatcacag atgagttatc taagaataca
     1801 gctttctgac cttcagctca cgcttcacag tgatcgatga ttcaggtgca gaggaagtac
     1861 aaactaaggt gctaaatctg cgatcatcgt catttgctgt gaactgaaat taaaactatt
     1921 catgctaccc agccattacc cagctaaata atcttactct agatacctaa aacataagat
     1981 cactgccaga gataaactaa tggtccacac ccaattccac tctgatagaa ttatttcaca
     2041 aataataggt gtttgtttaa tggacacttt tcacctcctt caattccata tatccttttc
     2101 ttctatgtag gaaaaaaata gtctagtgta gtattcttcc cttttaaaca cattttggtt
     2161 cttctcaaaa gaacttaccc ccattcgcgc tctgctcagt gggtaaaatt agatgcatgt
     2221 tgaccatgtc tatgattcgt gtaattactt atccctgccc atttcatatt ctttcaattc
     2281 tgtaggttaa aaaaatggca gtgatgaatt ttaagggttt cccccaacat ataaaataat
     2341 gcaaagaatc cctagacttt aggctttcag cttacacaaa tctatttaat taggaaaaaa
     2401 actattaagg atgtagcttt tcacctttac tttagaagca cagtaaatat cccaaactgt
     2461 gatgaagccg accttagatt tagtagtgta agctagaaga agtgagtgtt tctatgagtg
     2521 gaaaaagcca aggtgtcatt tatgtgtcag ttcatttgtg gtgatataga attagctttt
     2581 tcatcccctt acccttagaa tccacgtatt ccccttccca ctagcctagg tcataaagag
     2641 gcgttgcttt cactctccta tgccttttac gagtgctaag tgtaggatat tttgtcacca
     2701 gaacacaatg ctaccgcccc aaagtaaaag aaaccacttc tggtcaatca acactaccag
     2761 cgtatatata agaaagacat ctttctcttt tctaaaagac ttccctaaca cttaccccat
     2821 ggctgcacag ttggtggggt cctgccggag aggaagagac actcagacca gagaaggggt
     2881 gtgcatgcgc ctactcgctt gctagaagta gattctggac agtcagctct tcatctgccc
     2941 aactgtgtag catctgcatt gcccagtctt tcatgtgtgc caaggctgat gcaggatttg
     3001 ttctctgtcc agcagtcact tcggccagag ctgaagagtt gccccgtctc tgttccatgt
     3061 ctcctttaag agctctggtg ataaggacat gatgctttta ctgaactttc ttatcctagc
     3121 acatgcttca atagttcaag gaacttgaaa aatacttttc cttaccttta ccccatccct
     3181 gtctttactc ctcacactta ctgtaagaca ttagtaacat aataaattaa aagtcacacg
     3241 aatctcacca a
//
