LOCUS       NM_016247               8352 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens interphotoreceptor matrix proteoglycan 2 (IMPG2),
            mRNA.
ACCESSION   NM_016247
VERSION     NM_016247.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Birtel,J., Caswell,R., De Silva,S.R., Herrmann,P., Rehman,S.,
            Lotery,A.J., Mahroo,O.A., Michaelides,M., Webster,A.R.,
            MacLaren,R.E. and Charbel Issa,P.
  TITLE     IMPG2-Related Maculopathy
  JOURNAL   Am J Ophthalmol 258, 32-42 (2024)
   PUBMED   37806544
  REMARK    GeneRIF: IMPG2-Related Maculopathy.
REFERENCE   2  (bases 1 to 8352)
  AUTHORS   Vazquez-Dominguez,I., Li,C.H.Z., Fadaie,Z., Haer-Wigman,L.,
            Cremers,F.P.M., Garanto,A., Hoyng,C.B. and Roosing,S.
  TITLE     Identification of a Complex Allele in IMPG2 as a Cause of
            Adult-Onset Vitelliform Macular Dystrophy
  JOURNAL   Invest Ophthalmol Vis Sci 63 (5), 27 (2022)
   PUBMED   35608844
  REMARK    GeneRIF: Identification of a Complex Allele in IMPG2 as a Cause of
            Adult-Onset Vitelliform Macular Dystrophy.
REFERENCE   3  (bases 1 to 8352)
  AUTHORS   Chatterjee,S., Gupta,S., Chaudhry,V.N., Chaudhry,P., Mukherjee,A.
            and Mutsuddi,M.
  TITLE     Whole exome sequencing identifies a novel splice-site mutation in
            IMPG2 gene causing Stargardt-like juvenile macular dystrophy in a
            north Indian family
  JOURNAL   Gene 816, 146158 (2022)
   PUBMED   34990796
  REMARK    GeneRIF: Whole exome sequencing identifies a novel splice-site
            mutation in IMPG2 gene causing Stargardt-like juvenile macular
            dystrophy in a north Indian family.
REFERENCE   4  (bases 1 to 8352)
  AUTHORS   Datta,P., Hendrickson,B., Brendalen,S., Ruffcorn,A. and Seo,S.
  TITLE     The myosin-tail homology domain of centrosomal protein 290 is
            essential for protein confinement between the inner and outer
            segments in photoreceptors
  JOURNAL   J Biol Chem 294 (50), 19119-19136 (2019)
   PUBMED   31694913
  REMARK    GeneRIF: Upon disruption of the myosin-tail homology domain, inner
            segment plasma membrane proteins, including syntaxin 3 (STX3),
            synaptosome-associated protein 25 (SNAP25), and interphotoreceptor
            matrix proteoglycan 2 (IMPG2), rapidly accumulated in the outer
            segment.
REFERENCE   5  (bases 1 to 8352)
  AUTHORS   Khan,A.O. and Al Teneiji,A.M.
  TITLE     Homozygous and heterozygous retinal phenotypes in families
            harbouring IMPG2 mutations
  JOURNAL   Ophthalmic Genet 40 (3), 247-251 (2019)
   PUBMED   31264916
  REMARK    GeneRIF: IMPG2 mutation is associated with retinal dystrophy.
REFERENCE   6  (bases 1 to 8352)
  AUTHORS   Roni,V., Carpio,R. and Wissinger,B.
  TITLE     Mapping of transcription start sites of human retina expressed
            genes
  JOURNAL   BMC Genomics 8, 42 (2007)
   PUBMED   17286855
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 8352)
  AUTHORS   Kuehn,M.H., Stone,E.M. and Hageman,G.S.
  TITLE     Organization of the human IMPG2 gene and its evaluation as a
            candidate gene in age-related macular degeneration and other
            retinal degenerative disorders
  JOURNAL   Invest Ophthalmol Vis Sci 42 (13), 3123-3129 (2001)
   PUBMED   11726612
REFERENCE   8  (bases 1 to 8352)
  AUTHORS   Acharya,S., Foletta,V.C., Lee,J.W., Rayborn,M.E., Rodriguez,I.R.,
            Young,W.S. 3rd and Hollyfield,J.G.
  TITLE     SPACRCAN, a novel human interphotoreceptor matrix
            hyaluronan-binding proteoglycan synthesized by photoreceptors and
            pinealocytes
  JOURNAL   J Biol Chem 275 (10), 6945-6955 (2000)
   PUBMED   10702256
REFERENCE   9  (bases 1 to 8352)
  AUTHORS   Kuehn,M.H. and Hageman,G.S.
  TITLE     Molecular characterization and genomic mapping of human IPM 200, a
            second member of a novel family of proteoglycans
  JOURNAL   Mol Cell Biol Res Commun 2 (2), 103-110 (1999)
   PUBMED   10542133
  REMARK    GeneRIF: IPM 200 represents the second member of a novel family of
            retinal proteoglycans suspected to be involved in retinal adhesion
            and photoreceptor cell survival
REFERENCE   10 (bases 1 to 8352)
  AUTHORS   Fahim,A.T., Daiger,S.P. and Weleber,R.G.
  TITLE     Nonsyndromic Retinitis Pigmentosa Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301590
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX953286.1, AB593127.1,
            AC093008.10 and BM671068.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_016247.3.
            
            Summary: The protein encoded by this gene binds chondroitin sulfate
            and hyaluronan and is a proteoglycan. The encoded protein plays a
            role in the organization of the interphotoreceptor matrix and may
            promote the growth and maintenance of the light-sensitive
            photoreceptor outer segment. Defects in this gene are a cause of
            retinitis pigmentosa type 56 and maculopathy,
            IMPG2-related.[provided by RefSeq, Mar 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB593127.1, AF173155.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000193391.8/ ENSP00000193391.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 BX953286.1         2-10
            10-4448             AB593127.1         1-4439
            4449-8334           AC093008.10        133520-137405       c
            8335-8352           BM671068.1         14-31               c
FEATURES             Location/Qualifiers
     source          1..8352
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q12.3"
     gene            1..8352
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /note="interphotoreceptor matrix proteoglycan 2"
                     /db_xref="GeneID:50939"
                     /db_xref="HGNC:HGNC:18362"
                     /db_xref="MIM:607056"
     exon            1..288
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     CDS             204..3929
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /note="IPM 200; interphotoreceptor matrix proteoglycan of
                     200 kDa; sialoprotein associated with cones and rods
                     proteoglycan; interphotoreceptor matrix proteoglycan IPM
                     200"
                     /codon_start=1
                     /product="interphotoreceptor matrix proteoglycan 2
                     precursor"
                     /protein_id="NP_057331.2"
                     /db_xref="CCDS:CCDS2940.1"
                     /db_xref="GeneID:50939"
                     /db_xref="HGNC:HGNC:18362"
                     /db_xref="MIM:607056"
                     /translation="MIMFPLFGKISLGILIFVLIEGDFPSLTAQTYLSIEEIQEPKSA
                     VSFLLPEESTDLSLATKKKQPLDRRETERQWLIRRRRSILFPNGVKICPDESVAEAVA
                     NHVKYFKVRVCQEAVWEAFRTFWDRLPGREEYHYWMNLCEDGVTSIFEMGTNFSESVE
                     HRSLIMKKLTYAKETVSSSELSSPVPVGDTSTLGDTTLSVPHPEVDAYEGASESSLER
                     PEESISNEIENVIEEATKPAGEQIAEFSIHLLGKQYREELQDSSSFHHQHLEEEFISE
                     VENAFTGLPGYKEIRVLEFRSPKENDSGVDVYYAVTFNGEAISNTTWDLISLHSNKVE
                     NHGLVELDDKPTVVYTISNFRDYIAETLQQNFLLGNSSLNPDPDSLQLINVRGVLRHQ
                     TEDLVWNTQSSSLQATPSSILDNTFQAAWPSADESITSSIPPLDFSSGPPSATGRELW
                     SESPLGDLVSTHKLAFPSKMGLSSSPEVLEVSSLTLHSVTPAVLQTGLPVASEERTSG
                     SHLVEDGLANVEESEDFLSIDSLPSSSFTQPVPKETIPSMEDSDVSLTSSPYLTSSIP
                     FGLDSLTSKVKDQLKVSPFLPDASMEKELIFDGGLGSGSGQKVDLITWPWSETSSEKS
                     AEPLSKPWLEDDDSLLPAEIEDKKLVLVDKMDSTDQISKHSKYEHDDRSTHFPEEEPL
                     SGPAVPIFADTAAESASLTLPKHISEVPGVDDYSVTKAPLILTSVAISASTDKSDQAD
                     AILREDMEQITESSNYEWFDSEVSMVKPDMQTLWTILPESERVWTRTSSLEKLSRDIL
                     ASTPQSADRLWLSVTQSTKLPPTTISTLLEDEVIMGVQDISLELDRIGTDYYQPEQVQ
                     EQNGKVGSYVEMSTSVHSTEMVSVAWPTEGGDDLSYTQTSGALVVFFSLRVTNMMFSE
                     DLFNKNSLEYKALEQRFLELLVPYLQSNLTGFQNLEILNFRNGSIVVNSRMKFANSVP
                     PNVNNAVYMILEDFCTTAYNTMNLAIDKYSLDVESGDEANPCKFQACNEFSECLVNPW
                     SGEAKCRCFPGYLSVEERPCQSLCDLQPDFCLNDGKCDIMPGHGAICRCRVGENWWYR
                     GKHCEEFVSEPVIIGITIASVVGLLVIFSAIIYFFIRTLQAHHDRSERESPFSGSSRQ
                     PDSLSSIENAVKYNPVYESHRAGCEKYEGPYPQHPFYSSASGDVIGGLSREEIRQMYE
                     SSELSREEIQERMRVLELYANDPEFAAFVREQQVEEV"
     exon            289..537
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            538..704
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            705..736
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            737..786
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            787..869
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            870..1031
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1090
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1111
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1356
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1442
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1443..1746
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..3005
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3006..3225
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3226..3436
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3437..3625
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3626..3836
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3837..3916
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3917..8352
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8332..8337
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /note="hexamer: AATAAA"
     polyA_site      8352
                     /gene="IMPG2"
                     /gene_synonym="IPM200; RP56; SPACRCAN; VMD5"
                     /note="major polyA site"
ORIGIN      
        1 agaaaagaag tgagagctac tttgaaagga caaccatttt tctttccgct aatttataat
       61 ggttttgaag tggttgttca ttctcaaaca tagactttta aatgttaggt ctttcctata
      121 actctttgtt attggaagtt tcaaggattt ggacactcaa ttaaggattc tgtcctctcc
      181 tcattccttt ggttttggcc caaatgatta tgtttcctct ttttgggaag atttctctgg
      241 gtattttgat atttgtcctg atagaaggag actttccatc attaacagca caaacctact
      301 tatctataga ggagatccaa gaacccaaga gtgcagtttc ttttctcctg cctgaagaat
      361 caacagacct ttctctagct accaaaaaga aacagcctct ggaccgcaga gaaactgaaa
      421 gacagtggtt aatcagaagg cggagatcta ttctgtttcc taatggagtg aaaatctgcc
      481 cagatgaaag tgttgcagag gctgtggcaa atcatgtgaa gtattttaaa gtccgagtgt
      541 gtcaggaagc tgtctgggaa gccttcagga ctttttggga tcgacttcct gggcgtgagg
      601 aatatcatta ctggatgaat ttgtgtgagg atggagtcac aagtatattt gaaatgggca
      661 caaattttag tgaatctgtg gaacatagaa gcttaatcat gaagaaactg acttatgcaa
      721 aggaaactgt aagcagctct gaactgtctt ctccagttcc tgttggtgat acttcaacat
      781 tgggagacac tactctcagt gttccacatc cagaggtgga cgcctatgaa ggtgcctcag
      841 agagcagctt ggaaaggcca gaggagagta ttagcaatga aattgagaat gtgatagaag
      901 aagccacaaa accagcaggt gaacagattg cagaattcag tatccacctt ttggggaagc
      961 agtacaggga agaactacag gattcctcca gctttcacca ccagcacctt gaagaagaat
     1021 ttatttcaga ggttgaaaat gcatttactg ggttaccagg ctacaaggaa attcgtgtac
     1081 ttgaatttag gtcccccaag gaaaatgaca gtggcgtaga tgtttactat gcagttacct
     1141 tcaatggtga ggccatcagc aataccacct gggacctcat tagccttcac tccaacaagg
     1201 tggaaaacca tggccttgtg gaactggatg ataaacccac tgttgtttat acaatcagta
     1261 acttcagaga ttatattgct gagacattgc agcagaattt tttgctgggg aactcttcct
     1321 tgaatccaga tcctgattcc ctgcagctta tcaatgtgag aggagttttg cgtcaccaaa
     1381 ctgaagatct agtttggaac acccaaagtt caagtcttca ggcaacgccg tcatctattc
     1441 tggataatac ctttcaagct gcatggccct cagcagatga atccatcacc agcagtattc
     1501 caccacttga tttcagctct ggtcctccct cagccactgg cagggaactc tggtcagaaa
     1561 gtcctttggg tgatttagtg tctacacaca aattagcctt tccctcgaag atgggcctca
     1621 gctcttcccc agaggtttta gaggttagca gcttgactct tcattctgtc accccggcag
     1681 tgcttcagac tggcttgcct gtggcttctg aggaaaggac ttctggatct cacttggtag
     1741 aagatggatt agccaatgtt gaagagtcag aagattttct ttctattgat tcattgcctt
     1801 caagttcatt cactcaacct gtgccaaaag aaacaatacc atccatggaa gactctgatg
     1861 tgtccttaac atcttcacca tatctgacct cttctatacc ttttggcttg gactccttga
     1921 cctccaaagt caaagaccaa ttaaaagtga gccctttcct gccagatgca tccatggaaa
     1981 aagagttaat atttgacggt ggtttaggtt cagggtctgg gcaaaaggta gatctgatta
     2041 cttggccatg gagtgagact tcatcagaga agagcgctga accactgtcc aagccgtggc
     2101 ttgaagatga tgattcactt ttgccagctg agattgaaga caagaaacta gttttagttg
     2161 acaaaatgga ttccacagac caaattagta agcactcaaa atatgaacat gatgacagat
     2221 ccacacactt tccagaggaa gagcctctta gtgggcctgc tgtgcccatc ttcgcagata
     2281 ctgcagctga atctgcgtct ctaaccctcc ccaagcacat atcagaagta cctggtgttg
     2341 atgattactc agttaccaaa gcacctctta tactgacatc tgtagcaatc tctgcctcta
     2401 ctgataaatc agatcaggca gatgccatcc taagggagga tatggaacaa attactgagt
     2461 catccaacta tgaatggttt gacagtgagg tttcaatggt aaagccagat atgcaaactt
     2521 tgtggactat attgccagaa tcagagagag tttggacaag aacttcttcc ctagagaaat
     2581 tgtccagaga catattggca agtacaccac agagtgctga caggctctgg ttatctgtga
     2641 cacagtctac caaattgcct ccaaccacaa tctccaccct gctagaggat gaagtaatta
     2701 tgggtgtaca ggatatttcg ttagaactgg accggatagg cacagattac tatcagcctg
     2761 agcaagtcca agagcaaaat ggcaaggttg gtagttatgt ggaaatgtca acaagtgttc
     2821 actccacaga gatggttagt gtggcttggc ccacagaagg aggagatgac ttgagttata
     2881 cccagacttc aggagctttg gtggttttct tcagcctccg agtgactaac atgatgtttt
     2941 cagaagatct gtttaataaa aactccttgg agtataaagc cctggagcaa agattcttag
     3001 aattgctggt tccctatctc cagtcaaatc tcacggggtt ccagaactta gaaatcctca
     3061 acttcagaaa tggcagcatt gtggtgaaca gtcgaatgaa gtttgccaat tctgtccctc
     3121 ctaacgtcaa caatgcggtg tacatgattc tggaagactt ttgtaccact gcctacaata
     3181 ccatgaactt ggctattgat aaatactctc ttgatgtgga atcaggtgat gaagccaacc
     3241 cttgcaagtt tcaggcctgt aatgaatttt cagagtgtct ggtcaacccc tggagtggag
     3301 aagcaaagtg cagatgcttc cctggatacc tgagtgtgga agaacggccc tgtcagagtc
     3361 tctgtgacct acagcctgac ttctgcttga atgatggaaa gtgtgacatt atgcctgggc
     3421 acggggccat ttgtaggtgc cgggtgggtg agaactggtg gtaccgaggc aagcactgtg
     3481 aggaatttgt gtctgagccc gtgatcatag gcatcactat tgcctccgtg gttggacttc
     3541 ttgtcatctt ttctgctatc atctacttct tcatcaggac tctccaagca caccatgaca
     3601 ggagtgaaag agagagtccc ttcagtggct ccagcaggca gcctgacagc ctctcatcta
     3661 ttgagaatgc tgtgaagtac aaccccgtgt atgaaagtca cagggctgga tgtgagaagt
     3721 atgagggacc ctatcctcag catcccttct acagctctgc tagcggagac gtgattggtg
     3781 ggctgagcag agaagaaatc agacagatgt atgagagcag tgagctttcc agagaggaaa
     3841 ttcaagagag aatgagagtt ttggaactgt atgccaatga tcctgagttt gcagcttttg
     3901 tgagagagca acaagtggaa gaggtttaac caaaactcct gttctgaaac tgattagaag
     3961 cctggagaag atggagatta cttgttactt atgtcatata attaacctgg attttaaaca
     4021 ctgttggaag aagagttttc tatgaaaaaa ttaaatatag ggcacactgt ttttttttca
     4081 gcttaagttt tcagaatgta gtaagagatg ttaccatttt tatttctata aagactgaat
     4141 gctgtgttta aataaattga aaactacgta aactctggaa agtattctat aagaaaaagc
     4201 tggacctagg atttagggct gcagttgctg tctgcttctg aatcactggg ggtgtctcct
     4261 aagaactgtg cctcgggttt ttcaatagga aaataaggtg agactcttcc atggagaagt
     4321 ctggcttagt aagggaatat tttggagcat atttgttatt gttgggtaga aggggaaacc
     4381 tttgaaatag tcttaatgga tagtttataa tcaggcattc atctaaataa aattctttct
     4441 tttttttgat caaactgcta aagaaaaagc aaactttttt gctgcaggat agaattcggg
     4501 ttgtattttt atgcttctat cagtgtcttg catttcctga gcatattaat caagaccatg
     4561 ttattgttat gttaactatg ttaatatcct ttttttctta gttttcagga tgaatgcata
     4621 tatatatata tatatatata tatatatata tatatatata tatatatata taaagtttag
     4681 aagattccca ttaatctaaa gacaaattta atcacatggg cagggaggga gtgagtgtcg
     4741 ggtcaagacc ccagtgctga gaatatattt tgtttcttga ttgctggact gaggttccct
     4801 gattgcttct gaaatctaaa gatgtcttgg gcaaatcctg tctctcctct agttcctggt
     4861 gtattaattt ggaaaacagc acttaactac ctcacataat atagatgagc tcattttcat
     4921 tgcttctcct ttgtgtaata cctcctgtct aaattaactc ctgcttatac aaaacagggc
     4981 aaagctttta ttacaattaa aatattttaa aaactgaaca gataggaagt ccatacctta
     5041 tataaatttt gacagaaaat ggaaattttc ctctttcccc ctagtgtttt ttttgaatgg
     5101 gttgctttgg agggggagag gaggaggcca aatcatcatc atcatgatat taaattgcat
     5161 atgcaatagg ttattattaa tgcagatact ttatgctaga tgtcttcatg cttgccaaaa
     5221 ccaattctga acattttgat ttatttctga ccagatataa ataaccagta taaactgacc
     5281 ataagataaa atacttggtt cacttttttt tttctcaact tgatgactgc caattctgct
     5341 cattatgagg acaccggaaa ctaaatatct gggctcagtg catgccacag taaagctagt
     5401 gaaagccttg aagatggcca ggtattttgc aaagtatgta tggataacag gttgtgtgtg
     5461 tgtatgtgtg aatgtgtgtg tgcgtgtatg tgtgtgtgcg tgtgcgcgct aatgcgcatg
     5521 tgcgcccgcg ccaaggtggc tcccttatcc tagcatttcc tttcattccc tactcacata
     5581 atggatcctc taacagttca gtataggtac actttccact attttattga catgttctag
     5641 tcttaatatt ggataattag acactttcca ctgggcccat ttgaaaagta attgataatt
     5701 acctttaaga cattttaagt gttaatgata tttgtcacta ctaagtgctt agttttattt
     5761 ataaaacttt caaagttttc tgtttaagac tgtaaactgt tttcctatac tacgtgttag
     5821 atgaaagtgt attttatcac aaattaacct tagaatgcaa ctctttgaat gtgtgcttgc
     5881 tggaagaatt ccagatggac acaaatccct ctagggcagg tcaccatgca gaacacttag
     5941 gagaagcacg aactgagcca gaggagggtt gttgttttcc acaaactaag cagtgaagtg
     6001 ggaatttggg aattctgaca cacgtcacca tctctgattt ccttttgcgt gaccttttca
     6061 tgcaagagca aaacacttag ctcttcatga atgtctgctc cacaccaatg aaatggaaga
     6121 gtaaaggagc ccctcaacta tcagggaaag aacaggaggc ctcagttgag gaatacaact
     6181 tttgtcctgc ctttgctata gctgaaccac tgttgaaagg ttgaagaaca cagtttgccc
     6241 agcaaagatc catccactca tgtacaaagc aaagcactaa aggagctatt agtttgatgg
     6301 aaaaaactac ccctaaagag cttctttcta aaagaccttc ctcgaaggtg atttaatggc
     6361 atgtcatcat ttaaaagtct taggctgaac tgtcatttac actgcacaat tgaggaatga
     6421 aattttctga gtattagaac gttcttctta acctagcaat ataatattca acaagactat
     6481 caccttatgt gaaaatacta aaatgcagtt agcaataaaa tgcatggtaa ataaccattt
     6541 taaaaatgtt attataaaag gttgtaagat actgtggtgt tttacagatg tagcctttat
     6601 aattgaatta tatacactat ccttttttat gggttatgga taaaggggaa tttatttgat
     6661 aaatagggct ccattttcac cattgcaatg gtttaagggt gtttgttcac gtttatgtgg
     6721 cctgaagtcc catttttatc actattatta tttgagatgg agtctcgctc tgtcacccag
     6781 gctggagtgc aatggggcga tctcagctcc tggcaacctc tgcctcctgg gttcaagcga
     6841 ttctcgtgct tcagcttcct gagtagctgg ggttacagac acccaccacc atgcccagct
     6901 aatttttata tttttagtag agatggggtt tcaccgtcac catgttggcc agcctggtct
     6961 caaactcctg acctcaggtg atccacctgc cttggcctcc caaagtgctg ggattacagg
     7021 catgagccac cgcacctggc cccatttaat ttatataata aaacaagtgc aggctttacc
     7081 tgattttatt ctcaccttct gttttcttct tctgcattat ctgtcaccat gggttggctc
     7141 ctccccactt tctctttttt attcttttct ctcttttttg ccacactaaa ttgtaatgtg
     7201 tttgaacaac tctgacttaa tattacctct aacaaaaagc tggaaactgc acaaatattc
     7261 tttgtaggct ctaaaagtag gttggctgaa gtatgatacc tgaactaatt attcatttgt
     7321 atgcttgttt tataagtttg acttccattt catgattttt ttggtagctt tatgttattt
     7381 ctgttttcag caatgggtac tttgacatct ttctgtctct gaattgaatt ttcattttgc
     7441 aacggaagag aagacaacat atttattcca taatgaaaga gctaatgaag tagaataact
     7501 attaaattga gatgttttag caaaaaaaca aaaacacaaa ccccaaacgg gttgttttgt
     7561 tttatttttt tctaaaacat ttctaacaat tcccagtagg aatgggtggg gccagccttc
     7621 ctcatttaca atccagctgc agtgagtcaa gatcctagct tattctccca attgtgtaat
     7681 aaatgcagaa aaatgatgtg acggtcacta ttctcaaatc cattgcattg tattccacaa
     7741 aggatatcct ggcggaaggt ggaaatatgg gagggtacca tgatgtaaag aattgcctgg
     7801 aaatttcctt gagtcatgca ttttgtaaaa ccttacatta ctgctatgag ttaataagag
     7861 acaggttaaa ccactcccct caggatagta agactggtta aaaacactgc tctcagccca
     7921 cacagttata gctacagcct aggttaaatg attcctcatc ctggtgtttt tattctcatg
     7981 tttaattact tctttaaaga ttaagattat atatagaaaa aataattgtg gtatcagaga
     8041 atacaggtat aatttttcat atatcaggac tatgtgatga tacttaagca atagtgtaca
     8101 tacagtaata tatgtttaat atcgagtatg gataatgaag gacttttctt tttcaccttg
     8161 tttataatga cattttatcc agggtttgaa tcaaataaat tcatgttaac tgtactattt
     8221 tattgaaatg ttctagtctt attattggat aattagaaat aaacatttta aagtctttat
     8281 gaataaataa ggatgttttc ctatgtatac aactgtacta ttttcattag taataaacat
     8341 cactttcaag aa
//
