LOCUS       NM_030768               1455 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens ILK associated serine/threonine phosphatase (ILKAP),
            mRNA.
ACCESSION   NM_030768
VERSION     NM_030768.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Zhang,R., Yuan,J., Liu,S., Torraca,V., Liao,Z., Wu,Y., Tan,H.,
            Yao,X., Hou,X., Lyu,H., Xiao,S., Guo,D., Ali,D.W., Michalak,M.,
            Chen,X.Z., Zhou,C. and Tang,J.
  TITLE     ILKAP Promotes the Metastasis of Hepatocellular Carcinoma Cells by
            Inhibiting beta-Catenin Degradation and Enhancing the WNT Signaling
            Pathway
  JOURNAL   Adv Biol (Weinh) 8 (5), e2300117 (2024)
   PUBMED   38379270
  REMARK    GeneRIF: ILKAP Promotes the Metastasis of Hepatocellular Carcinoma
            Cells by Inhibiting beta-Catenin Degradation and Enhancing the WNT
            Signaling Pathway.
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 1455)
  AUTHORS   Liu,T., Liu,Y., Cao,J., Gao,X., Wang,J., Chen,G., Wang,Y., Liu,G.,
            Bai,G., Hu,Y., Yang,J., Dong,L., Quan,X. and Zou,W.
  TITLE     ILKAP Binding to and Dephosphorylating HIF-1alpha is Essential for
            Apoptosis Induced by Severe Hypoxia
  JOURNAL   Cell Physiol Biochem 46 (6), 2500-2507 (2018)
   PUBMED   29742494
  REMARK    GeneRIF: ILKAP physically interacted with HIF-1A and induced its
            dephosphorylation. Both the HIF-1A-p53 interaction and apoptosis
            relied on ILKAP.
REFERENCE   4  (bases 1 to 1455)
  AUTHORS   Hausmann,C., Temme,A., Cordes,N. and Eke,I.
  TITLE     ILKAP, ILK and PINCH1 control cell survival of p53-wildtype
            glioblastoma cells after irradiation
  JOURNAL   Oncotarget 6 (33), 34592-34605 (2015)
   PUBMED   26460618
  REMARK    GeneRIF: our data suggest an essential role of PINCH1, ILK and
            ILKAP for the radioresistance of p53-wildtype glioblastoma
            multiforme cells
REFERENCE   5  (bases 1 to 1455)
  AUTHORS   Shao,G., Zheng,Q., Xu,X., Zhao,J. and Liu,P.
  TITLE     Endometrial ILKAP expression among patients with endometriosis and
            its association with clinical characteristics
  JOURNAL   Int J Gynaecol Obstet 130 (1), 23-26 (2015)
   PUBMED   25872452
  REMARK    GeneRIF: The expression of ILKAP was considerably lower in eutopic
            and ectopic endometrial samples from women with endometriosis than
            in control endometrium. Expression varied depending on the phase of
            the menstrual cycle.
REFERENCE   6  (bases 1 to 1455)
  AUTHORS   Zhou,W., Cui,S., Han,S., Cheng,B., Zheng,Y. and Zhang,Y.
  TITLE     Palladin is a novel binding partner of ILKAP in eukaryotic cells
  JOURNAL   Biochem Biophys Res Commun 411 (4), 768-773 (2011)
   PUBMED   21782789
  REMARK    GeneRIF: these results suggested that palladin played a specific
            role in modulating the subcellular localization of the cytoplasmic
            ILKAP and promoting the ILKAP-induced apoptosis.
REFERENCE   7  (bases 1 to 1455)
  AUTHORS   Nakrieko,K.A., Vespa,A., Mason,D., Irvine,T.S., D'Souza,S.J. and
            Dagnino,L.
  TITLE     Modulation of integrin-linked kinase nucleo-cytoplasmic shuttling
            by ILKAP and CRM1
  JOURNAL   Cell Cycle 7 (14), 2157-2166 (2008)
   PUBMED   18635968
REFERENCE   8  (bases 1 to 1455)
  AUTHORS   Kumar,A.S., Naruszewicz,I., Wang,P., Leung-Hagesteijn,C. and
            Hannigan,G.E.
  TITLE     ILKAP regulates ILK signaling and inhibits anchorage-independent
            growth
  JOURNAL   Oncogene 23 (19), 3454-3461 (2004)
   PUBMED   14990992
REFERENCE   9  (bases 1 to 1455)
  AUTHORS   Leung-Hagesteijn,C., Mahendra,A., Naruszewicz,I. and Hannigan,G.E.
  TITLE     Modulation of integrin signal transduction by ILKAP, a protein
            phosphatase 2C associating with the integrin-linked kinase, ILK1
  JOURNAL   EMBO J 20 (9), 2160-2170 (2001)
   PUBMED   11331582
REFERENCE   10 (bases 1 to 1455)
  AUTHORS   Tong,Y., Quirion,R. and Shen,S.H.
  TITLE     Cloning and characterization of a novel mammalian PP2C isozyme
  JOURNAL   J Biol Chem 273 (52), 35282-35290 (1998)
   PUBMED   9857069
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC016757.10.
            
            On Nov 26, 2018 this sequence version replaced NM_030768.2.
            
            Summary: The protein encoded by this gene is a protein
            serine/threonine phosphatase of the PP2C family. This protein can
            interact with integrin-linked kinase (ILK/ILK1), a regulator of
            integrin mediated signaling, and regulate the kinase activity of
            ILK. Through the interaction with ILK, this protein may selectively
            affect the signaling process of ILK-mediated glycogen synthase
            kinase 3 beta (GSK3beta), and thus participate in Wnt signaling
            pathway. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK001043.1, BC006576.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000254654.8/ ENSP00000254654.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-197               AC016757.10        117718-117914       c
            198-263             AC016757.10        109024-109089       c
            264-320             AC016757.10        108494-108550       c
            321-440             AC016757.10        104072-104191       c
            441-567             AC016757.10        102350-102476       c
            568-674             AC016757.10        99400-99506         c
            675-768             AC016757.10        98239-98332         c
            769-856             AC016757.10        97872-97959         c
            857-978             AC016757.10        96284-96405         c
            979-1098            AC016757.10        87753-87872         c
            1099-1180           AC016757.10        85162-85243         c
            1181-1455           AC016757.10        84621-84895         c
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.3"
     gene            1..1455
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /note="ILK associated serine/threonine phosphatase"
                     /db_xref="GeneID:80895"
                     /db_xref="HGNC:HGNC:15566"
                     /db_xref="MIM:618909"
     exon            1..197
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..1321
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /EC_number="3.1.3.16"
                     /note="protein phosphatase 2c, delta isozyme;
                     integrin-linked kinase associated phosphatase; protein
                     phosphatase, Mg2+/Mn2+ dependent 1O"
                     /codon_start=1
                     /product="integrin-linked kinase-associated
                     serine/threonine phosphatase 2C"
                     /protein_id="NP_110395.1"
                     /db_xref="CCDS:CCDS2526.1"
                     /db_xref="GeneID:80895"
                     /db_xref="HGNC:HGNC:15566"
                     /db_xref="MIM:618909"
                     /translation="MDLFGDLPEPERSPRPAAGKEAQKGPLLFDDLPPASSTDSGSGG
                     PLLFDDLPPASSGDSGSLATSISQMVKTEGKGAKRKTSEEEKNGSEELVEKKVCKASS
                     VIFGLKGYVAERKGEREEMQDAHVILNDITEECRPPSSLITRVSYFAVFDGHGGIRAS
                     KFAAQNLHQNLIRKFPKGDVISVEKTVKRCLLDTFKHTDEEFLKQASSQKPAWKDGST
                     ATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGG
                     NVRDGRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTP
                     EEAVNFILSCLEDEKIQTREGKSAADARYEAACNRLANKAVQRGSADNVTVMVVRIGH
                     "
     exon            198..263
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /inference="alignment:Splign:2.1.0"
     exon            264..320
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /inference="alignment:Splign:2.1.0"
     exon            321..440
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /inference="alignment:Splign:2.1.0"
     exon            441..567
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /inference="alignment:Splign:2.1.0"
     exon            568..674
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /inference="alignment:Splign:2.1.0"
     exon            675..768
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /inference="alignment:Splign:2.1.0"
     exon            769..856
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /inference="alignment:Splign:2.1.0"
     exon            857..978
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1098
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1180
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1455
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1429..1434
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /note="hexamer: AATAAA"
     polyA_site      1455
                     /gene="ILKAP"
                     /gene_synonym="ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O"
                     /note="major polyA site"
ORIGIN      
        1 actctccctg caggtgactg acggcgccgg ccgcccctgc ccgtcgcccg cccgctgctg
       61 ccgcccgccc ggggtgtgga gcccggccgc tgctcgcggg ctgagtgtct gtcgctgctg
      121 ccgcctccac ccagcctccg ccatggacct cttcggggac ctgccggagc ccgagcgctc
      181 gccgcgcccg gctgccggga aagaagctca gaaaggaccc ctgctctttg atgacctccc
      241 tccggccagc agtactgact caggatcagg gggacctttg ctttttgatg atctcccacc
      301 cgctagcagt ggcgattcag gttctcttgc cacatcaata tcccagatgg taaagactga
      361 agggaaagga gcaaagagaa aaacctccga ggaagagaag aatggcagtg aagagcttgt
      421 ggaaaagaaa gtttgtaaag cctcttcggt gatctttggt ctgaagggct atgtggctga
      481 gcggaagggt gagagggagg agatgcagga tgcccacgtc atcctgaacg acatcaccga
      541 ggagtgtagg cccccatcgt ccctcattac tcgggtttca tattttgctg tttttgatgg
      601 acatggagga attcgagcct caaaatttgc tgcacagaat ttgcatcaaa acttaatcag
      661 aaaatttcct aaaggagatg taatcagtgt agagaaaacc gtgaagagat gccttttgga
      721 cactttcaag catactgatg aagagttcct taaacaagct tccagccaga agcctgcctg
      781 gaaagatggg tccactgcca cgtgtgttct ggctgtagac aacattcttt atattgccaa
      841 cctcggagat agtcgggcaa tcttgtgtcg ttataatgag gagagtcaaa aacatgcagc
      901 cttaagcctc agcaaagagc ataatccaac tcagtatgaa gagcggatga ggatacagaa
      961 ggctggagga aacgtcaggg atgggcgtgt tttgggcgtg ctagaggtgt cacgctccat
     1021 tggggacggg cagtacaagc gctgcggtgt cacctctgtg cccgacatca gacgctgcca
     1081 gctgaccccc aatgacaggt tcattttgtt ggcctgtgat gggctcttca aggtctttac
     1141 cccagaagaa gccgtgaact tcatcttgtc ctgtctcgag gatgaaaaga tccagacccg
     1201 ggaagggaag tccgcagccg acgcccgcta cgaagcagcc tgcaacaggc tggccaacaa
     1261 ggcggtgcag cggggctcgg ccgacaacgt cactgtgatg gtggtgcgga tagggcactg
     1321 aggggtggcg cgcggccagg agcacgcatg gtattgactt aaaaggttca ttttgtgtgt
     1381 gtgcacattg tgtgttttgt gtactcctgt gggactccca tggttgtaaa taaaggtttc
     1441 tctttttttt tccta
//
