LOCUS       NM_004517               1759 bp    mRNA    linear   PRI 08-DEC-2024
DEFINITION  Homo sapiens integrin linked kinase (ILK), transcript variant 1,
            mRNA.
ACCESSION   NM_004517
VERSION     NM_004517.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Bildyug,N.
  TITLE     Inhibition of Integrin-Associated Kinases FAK and ILK on the In
            Vitro Model of Skin Wound Healing
  JOURNAL   Appl Biochem Biotechnol 196 (8), 5604-5615 (2024)
   PUBMED   38165590
  REMARK    GeneRIF: Inhibition of Integrin-Associated Kinases FAK and ILK on
            the In Vitro Model of Skin Wound Healing.
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Campillo,S., Gutierrez-Calabres,E., Garcia-Miranda,S., Griera,M.,
            Fernandez Rodriguez,L., de Frutos,S., Rodriguez-Puyol,D. and
            Calleros,L.
  TITLE     Integrin-linked kinase mRNA expression in circulating mononuclear
            cells as a biomarker of kidney and vascular damage in experimental
            chronic kidney disease
  JOURNAL   Cell Commun Signal 22 (1), 264 (2024)
   PUBMED   38734696
  REMARK    GeneRIF: Integrin-linked kinase mRNA expression in circulating
            mononuclear cells as a biomarker of kidney and vascular damage in
            experimental chronic kidney disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1759)
  AUTHORS   Ahmed,A.U., Almasabi,S., Firestein,R. and Williams,B.R.G.
  TITLE     Integrin-linked kinase expression in myeloid cells promotes colon
            tumorigenesis
  JOURNAL   Front Immunol 14, 1270194 (2023)
   PUBMED   38077324
  REMARK    GeneRIF: Integrin-linked kinase expression in myeloid cells
            promotes colon tumorigenesis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1759)
  AUTHORS   Huang,Q., Sun,Y., Peng,L., Sun,J., Sha,Z., Lin,H., Li,Y., Li,C.,
            Li,H., Shang,H., Chen,Y., Dou,X., Hu,Z., Ye,Z. and Peng,H.
  TITLE     Extracellular vesicle-packaged ILK from mesothelial cells promotes
            fibroblast activation in peritoneal fibrosis
  JOURNAL   J Extracell Vesicles 12 (7), e12334 (2023)
   PUBMED   37357686
  REMARK    GeneRIF: Extracellular vesicle-packaged ILK from mesothelial cells
            promotes fibroblast activation in peritoneal fibrosis.
REFERENCE   5  (bases 1 to 1759)
  AUTHORS   Hannigan,G.E., McDonald,P.C., Walsh,M.P. and Dedhar,S.
  TITLE     Integrin-linked kinase: not so 'pseudo' after all
  JOURNAL   Oncogene 30 (43), 4375-4385 (2011)
   PUBMED   21602880
  REMARK    Review article
REFERENCE   6  (bases 1 to 1759)
  AUTHORS   Melchior,C., Kreis,S., Janji,B. and Kieffer,N.
  TITLE     Promoter characterization and genomic organization of the gene
            encoding integrin-linked kinase 1
  JOURNAL   Biochim Biophys Acta 1575 (1-3), 117-122 (2002)
   PUBMED   12020826
REFERENCE   7  (bases 1 to 1759)
  AUTHORS   Delcommenne,M., Tan,C., Gray,V., Rue,L., Woodgett,J. and Dedhar,S.
  TITLE     Phosphoinositide-3-OH kinase-dependent regulation of glycogen
            synthase kinase 3 and protein kinase B/AKT by the integrin-linked
            kinase
  JOURNAL   Proc Natl Acad Sci U S A 95 (19), 11211-11216 (1998)
   PUBMED   9736715
REFERENCE   8  (bases 1 to 1759)
  AUTHORS   Li,F., Liu,J., Mayne,R. and Wu,C.
  TITLE     Identification and characterization of a mouse protein kinase that
            is highly homologous to human integrin-linked kinase
  JOURNAL   Biochim Biophys Acta 1358 (3), 215-220 (1997)
   PUBMED   9366252
REFERENCE   9  (bases 1 to 1759)
  AUTHORS   Hannigan,G.E., Bayani,J., Weksberg,R., Beatty,B., Pandita,A.,
            Dedhar,S. and Squire,J.
  TITLE     Mapping of the gene encoding the integrin-linked kinase, ILK, to
            human chromosome 11p15.5-p15.4
  JOURNAL   Genomics 42 (1), 177-179 (1997)
   PUBMED   9177792
REFERENCE   10 (bases 1 to 1759)
  AUTHORS   Hannigan,G.E., Leung-Hagesteijn,C., Fitz-Gibbon,L., Coppolino,M.G.,
            Radeva,G., Filmus,J., Bell,J.C. and Dedhar,S.
  TITLE     Regulation of cell adhesion and anchorage-dependent growth by a new
            beta 1-integrin-linked protein kinase
  JOURNAL   Nature 379 (6560), 91-96 (1996)
   PUBMED   8538749
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC380243.1, U40282.1 and
            HY257434.1.
            
            On Jun 2, 2019 this sequence version replaced NM_004517.3.
            
            Summary: This gene encodes a protein with a kinase-like domain and
            four ankyrin-like repeats. The encoded protein associates at the
            cell membrane with the cytoplasmic domain of beta integrins, where
            it regulates integrin-mediated signal transduction. Activity of
            this protein is important in the epithelial to mesenchymal
            transition, and over-expression of this gene is implicated in tumor
            growth and metastasis. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Jun 2013].
            
            Transcript Variant: This variant (1) encodes isoform 1. Variants 1,
            2, and 3 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.37999.1,
                                           SRR1803611.127614.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299421.9/ ENSP00000299421.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 DC380243.1         67-74
            9-1620              U40282.1           24-1635
            1621-1759           HY257434.1         35-173              c
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.4"
     gene            1..1759
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /note="integrin linked kinase"
                     /db_xref="GeneID:3611"
                     /db_xref="HGNC:HGNC:6040"
                     /db_xref="MIM:602366"
     exon            1..49
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            50..230
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     CDS             142..1500
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     integrin-linked kinase-2; 59 kDa serine/threonine-protein
                     kinase; integrin-linked protein kinase; epididymis
                     secretory protein Li 28; beta-integrin-linked kinase;
                     inactive integrin-linked kinase; scaffold protein ILK"
                     /codon_start=1
                     /product="scaffold protein ILK isoform 1"
                     /protein_id="NP_004508.1"
                     /db_xref="CCDS:CCDS7768.1"
                     /db_xref="GeneID:3611"
                     /db_xref="HGNC:HGNC:6040"
                     /db_xref="MIM:602366"
                     /translation="MDDIFTQCREGNAVAVRLWLDNTENDLNQGDDHGFSPLHWACRE
                     GRSAVVEMLIMRGARINVMNRGDDTPLHLAASHGHRDIVQKLLQYKADINAVNEHGNV
                     PLHYACFWGQDQVAEDLVANGALVSICNKYGEMPVDKAKAPLRELLRERAEKMGQNLN
                     RIPYKDTFWKGTTRTRPRNGTLNKHSGIDFKQLNFLTKLNENHSGELWKGRWQGNDIV
                     VKVLKVRDWSTRKSRDFNEECPRLRIFSHPNVLPVLGACQSPPAPHPTLITHWMPYGS
                     LYNVLHEGTNFVVDQSQAVKFALDMARGMAFLHTLEPLIPRHALNSRSVMIDEDMTAR
                     ISMADVKFSFQCPGRMYAPAWVAPEALQKKPEDTNRRSADMWSFAVLLWELVTREVPF
                     ADLSNMEIGMKVALEGLRPTIPPGISPHVCKLMKICMNEDPAKRPKFDMIVPILEKMQ
                     DK"
     exon            231..396
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            397..492
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            493..589
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            590..673
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            674..759
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            760..869
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            870..997
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1119
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1219
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1350
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1759
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1740..1745
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /note="hexamer: AATAAA"
     polyA_site      1759
                     /gene="ILK"
                     /gene_synonym="HEL-S-28; ILK-1; ILK-2; P59; p59ILK"
                     /note="major polyA site"
ORIGIN      
        1 gcggccggac gggagttccc cggagaagga tcctgcagcc cgagtcccga ggataaagct
       61 tggggttcat cctccttccc tggatcactc cacagtcctc aggcttcccc aatccagggg
      121 actcggcgcc gggacgctgc tatggacgac attttcactc agtgccggga gggcaacgca
      181 gtcgccgttc gcctgtggct ggacaacacg gagaacgacc tcaaccaggg ggacgatcat
      241 ggcttctccc ccttgcactg ggcctgccga gagggccgct ctgctgtggt tgagatgttg
      301 atcatgcggg gggcacggat caatgtaatg aaccgtgggg atgacacccc cctgcatctg
      361 gcagccagtc atggacaccg tgatattgta cagaagctat tgcagtacaa ggcagacatc
      421 aatgcagtga atgaacacgg gaatgtgccc ctgcactatg cctgtttttg gggccaagat
      481 caagtggcag aggacctggt ggcaaatggg gcccttgtca gcatctgtaa caagtatgga
      541 gagatgcctg tggacaaagc caaggcaccc ctgagagagc ttctccgaga gcgggcagag
      601 aagatgggcc agaatctcaa ccgtattcca tacaaggaca cattctggaa ggggaccacc
      661 cgcactcggc cccgaaatgg aaccctgaac aaacactctg gcattgactt caaacagctt
      721 aacttcctga cgaagctcaa cgagaatcac tctggagagc tatggaaggg ccgctggcag
      781 ggcaatgaca ttgtcgtgaa ggtgctgaag gttcgagact ggagtacaag gaagagcagg
      841 gacttcaatg aagagtgtcc ccggctcagg attttctcgc atccaaatgt gctcccagtg
      901 ctaggtgcct gccagtctcc acctgctcct catcctactc tcatcacaca ctggatgccg
      961 tatggatccc tctacaatgt actacatgaa ggcaccaatt tcgtcgtgga ccagagccag
     1021 gctgtgaagt ttgctttgga catggcaagg ggcatggcct tcctacacac actagagccc
     1081 ctcatcccac gacatgcact caatagccgt agtgtaatga ttgatgagga catgactgcc
     1141 cgaattagca tggctgatgt caagttctct ttccaatgtc ctggtcgcat gtatgcacct
     1201 gcctgggtag cccccgaagc tctgcagaag aagcctgaag acacaaacag acgctcagca
     1261 gacatgtgga gttttgcagt gcttctgtgg gaactggtga cacgggaggt accctttgct
     1321 gacctctcca atatggagat tggaatgaag gtggcattgg aaggccttcg gcctaccatc
     1381 ccaccaggta tttcccctca tgtgtgtaag ctcatgaaga tctgcatgaa tgaagaccct
     1441 gcaaagcgac ccaaatttga catgattgtg cctatccttg agaagatgca ggacaagtag
     1501 gactggaagg tccttgcctg aactccagag gtgtcgggac atggttgggg gaatgcacct
     1561 ccccaaagca gcaggcctct ggttgcctcc cccgcctcca gtcatggtac taccccagcc
     1621 atggggtcca tccccttccc ccatccctac cactgtggcc ccaagagggg cgggctcaga
     1681 gctttgtcac ttgccacatg gtgtctccca acatgggagg gatcagcccc gcctgtcaca
     1741 ataaagttta ttatgaaaa
//
