LOCUS       NM_004515               1852 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens interleukin enhancer binding factor 2 (ILF2),
            transcript variant 1, mRNA.
ACCESSION   NM_004515
VERSION     NM_004515.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Liu,S.S., Wan,Q.S., Lv,C., Wang,J.K., Jiang,S., Cai,D., Liu,M.S.,
            Wang,T. and Zhang,K.H.
  TITLE     Integrating trans-omics, cellular experiments and clinical
            validation to identify ILF2 as a diagnostic serum biomarker and
            therapeutic target in gastric cancer
  JOURNAL   BMC Cancer 24 (1), 465 (2024)
   PUBMED   38622522
  REMARK    GeneRIF: Integrating trans-omics, cellular experiments and clinical
            validation to identify ILF2 as a diagnostic serum biomarker and
            therapeutic target in gastric cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Chiu,C.L., Li,C.G., Verschueren,E., Wen,R.M., Zhang,D.,
            Gordon,C.A., Zhao,H., Giaccia,A.J. and Brooks,J.D.
  TITLE     NUSAP1 Binds ILF2 to Modulate R-Loop Accumulation and DNA Damage in
            Prostate Cancer
  JOURNAL   Int J Mol Sci 24 (7), 6258 (2023)
   PUBMED   37047232
  REMARK    GeneRIF: NUSAP1 Binds ILF2 to Modulate R-Loop Accumulation and DNA
            Damage in Prostate Cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1852)
  AUTHORS   Zhang,H., Che,Y., Xuan,B., Wu,X. and Li,H.
  TITLE     Serine hydroxymethyltransferase 2 (SHMT2) potentiates the
            aggressive process of oral squamous cell carcinoma by binding to
            interleukin enhancer-binding factor 2 (ILF2)
  JOURNAL   Bioengineered 13 (4), 8785-8797 (2022)
   PUBMED   35333683
  REMARK    GeneRIF: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the
            aggressive process of oral squamous cell carcinoma by binding to
            interleukin enhancer-binding factor 2 (ILF2).
REFERENCE   4  (bases 1 to 1852)
  AUTHORS   Kazuma,Y., Shirakawa,K., Tashiro,Y., Yamazaki,H., Nomura,R.,
            Horisawa,Y., Takeuchi,S., Stanford,E., Konishi,Y., Matsui,H.,
            Matsumoto,T., Tanabe,F., Morishita,R., Ito,S. and Takaori-Kondo,A.
  TITLE     ILF2 enhances the DNA cytosine deaminase activity of tumor mutator
            APOBEC3B in multiple myeloma cells
  JOURNAL   Sci Rep 12 (1), 2278 (2022)
   PUBMED   35145187
  REMARK    GeneRIF: ILF2 enhances the DNA cytosine deaminase activity of tumor
            mutator APOBEC3B in multiple myeloma cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1852)
  AUTHORS   Shiu,T.Y., Lin,H.H., Shih,Y.L., Feng,A.C., Huang,H.H., Huang,T.Y.,
            Hsieh,C.B., Chang,W.K. and Hsieh,T.Y.
  TITLE     CRNDE-h transcript/miR-136-5p axis regulates interleukin enhancer
            binding factor 2 expression to promote hepatocellular carcinoma
            cell proliferation
  JOURNAL   Life Sci 284, 119708 (2021)
   PUBMED   34153299
  REMARK    GeneRIF: CRNDE-h transcript/miR-136-5p axis regulates interleukin
            enhancer binding factor 2 expression to promote hepatocellular
            carcinoma cell proliferation.
REFERENCE   6  (bases 1 to 1852)
  AUTHORS   Satoh,M., Shaheen,V.M., Kao,P.N., Okano,T., Shaw,M., Yoshida,H.,
            Richards,H.B. and Reeves,W.H.
  TITLE     Autoantibodies define a family of proteins with conserved
            double-stranded RNA-binding domains as well as DNA binding activity
  JOURNAL   J Biol Chem 274 (49), 34598-34604 (1999)
   PUBMED   10574923
REFERENCE   7  (bases 1 to 1852)
  AUTHORS   Langland,J.O., Kao,P.N. and Jacobs,B.L.
  TITLE     Nuclear factor-90 of activated T-cells: A double-stranded
            RNA-binding protein and substrate for the double-stranded
            RNA-dependent protein kinase, PKR
  JOURNAL   Biochemistry 38 (19), 6361-6368 (1999)
   PUBMED   10320367
REFERENCE   8  (bases 1 to 1852)
  AUTHORS   Ting,N.S., Kao,P.N., Chan,D.W., Lintott,L.G. and Lees-Miller,S.P.
  TITLE     DNA-dependent protein kinase interacts with antigen receptor
            response element binding proteins NF90 and NF45
  JOURNAL   J Biol Chem 273 (4), 2136-2145 (1998)
   PUBMED   9442054
REFERENCE   9  (bases 1 to 1852)
  AUTHORS   Marcoulatos,P., Koussidis,G., Mamuris,Z., Velissariou,V. and
            Vamvakopoulos,N.C.
  TITLE     Mapping interleukin enhancer binding factor 2 gene (ILF2) to human
            chromosome 1 (1q11-qter and 1p11-p12) by polymerase chain reaction
            amplification of human-rodent somatic cell hybrid DNA templates
  JOURNAL   J Interferon Cytokine Res 16 (12), 1035-1038 (1996)
   PUBMED   8974006
REFERENCE   10 (bases 1 to 1852)
  AUTHORS   Kao,P.N., Chen,L., Brock,G., Ng,J., Kenny,J., Smith,A.J. and
            Corthesy,B.
  TITLE     Cloning and expression of cyclosporin A- and FK506-sensitive
            nuclear factor of activated T-cells: NF45 and NF90
  JOURNAL   J Biol Chem 269 (32), 20691-20699 (1994)
   PUBMED   7519613
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC412988.1, AK223326.1,
            BC000382.2 and BX450311.2.
            
            On Nov 23, 2018 this sequence version replaced NM_004515.3.
            
            Summary: The protein encoded by this gene is a transcription factor
            required for T-cell expression of the interleukin 2 gene. It also
            binds RNA and is an essential component for encapsidation and
            protein priming of hepatitis B viral polymerase. The encoded 45 kDa
            protein (NF45, ILF2) forms a complex with the 90 kDa interleukin
            enhancer-binding factor 3 (NF90, ILF3), and this complex has been
            shown to affect the redistribution of nuclear mRNA to the
            cytoplasm, to repair DNA breaks by nonhomologous end joining, and
            to negatively regulate the microRNA processing pathway. Knockdown
            of NF45 or NF90 protein retards cell growth, possibly by inhibition
            of mRNA stabilization. Alternative splicing results in multiple
            transcript variants. Related pseudogenes have been found on
            chromosomes 3 and 14. [provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853558.1761.1, BC000382.2
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361891.9/ ENSP00000355011.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                DC412988.1         36-80
            46-323              AK223326.1         1-278
            324-930             BC000382.2         284-890
            931-1607            AK223326.1         886-1562
            1608-1852           BX450311.2         88-332
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q21.3"
     gene            1..1852
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /note="interleukin enhancer binding factor 2"
                     /db_xref="GeneID:3608"
                     /db_xref="HGNC:HGNC:6037"
                     /db_xref="MIM:603181"
     exon            1..76
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..1244
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /note="isoform 1 is encoded by transcript variant 1;
                     nuclear factor of activated T-cells, 45-kDa; interleukin
                     enhancer binding factor 2, 45kDa"
                     /codon_start=1
                     /product="interleukin enhancer-binding factor 2 isoform 1"
                     /protein_id="NP_004506.2"
                     /db_xref="CCDS:CCDS1050.1"
                     /db_xref="GeneID:3608"
                     /db_xref="HGNC:HGNC:6037"
                     /db_xref="MIM:603181"
                     /translation="MRGDRGRGRGGRFGSRGGPGGGFRPFVPHIPFDFYLCEMAFPRV
                     KPAPDETSFSEALLKRNQDLAPNSAEQASILSLVTKINNVIDNLIVAPGTFEVQIEEV
                     RQVGSYKKGTMTTGHNVADLVVILKILPTLEAVAALGNKVVESLRAQDPSEVLTMLTN
                     ETGFEISSSDATVKILITTVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENASQS
                     TVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILA
                     AGLFLPGSVGITDPCESGNFRVHTVMTLEQQDMVCYTAQTLVRILSHGGFRKILGQEG
                     DASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEEEENTEEPPQGEEEESMETQE"
     exon            77..136
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     exon            137..179
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     exon            180..284
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     exon            285..362
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     exon            363..465
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     exon            466..531
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     exon            532..648
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     exon            649..727
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     exon            728..815
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     exon            816..877
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     exon            878..992
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1083
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1852
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1584..1589
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /note="hexamer: ATTAAA"
     polyA_site      1606
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /note="major polyA site"
     regulatory      1721..1726
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /note="hexamer: TATAAA"
     polyA_site      1740
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
     regulatory      1796..1801
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /note="hexamer: AGTAAA"
     regulatory      1829..1834
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
                     /note="hexamer: AATAAA"
     polyA_site      1838
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
     polyA_site      1852
                     /gene="ILF2"
                     /gene_synonym="NF45; PRO3063"
ORIGIN      
        1 ctcttcagtt gtctgctact cagaggaagg ggcggttggt gcggcctcca ttgttcgtgt
       61 tttaaggcgc catgaggggt gacagaggcc gtggtcgtgg tgggcgcttt ggttccagag
      121 gaggcccagg aggagggttc aggccctttg taccacatat cccatttgac ttctatttgt
      181 gtgaaatggc ctttccccgg gtcaagccag cacctgatga aacttccttc agtgaggcct
      241 tgctgaagag gaatcaggac ctggctccca attctgctga acaggcatct atcctttctc
      301 tggtgacaaa aataaacaat gtgattgata atctgattgt ggctccaggg acatttgaag
      361 tgcaaattga agaagttcga caggtgggat cctataaaaa ggggacaatg actacaggac
      421 acaatgtggc tgacctggtg gtgatactca agattctgcc aacgttggaa gctgttgctg
      481 ccctggggaa caaagtcgtg gaaagcctaa gagcacagga tccttctgaa gttttaacca
      541 tgctgaccaa cgaaactggc tttgaaatca gttcttctga tgctacagtg aagattctca
      601 ttacaacagt gccacccaat cttcgaaaac tggatccaga actccatttg gatatcaaag
      661 tattgcagag tgccttagca gccatccgac atgcccgctg gttcgaggaa aatgcttctc
      721 agtccacagt taaagttctc atcagactac tgaaggactt gaggattcgt tttcctggct
      781 ttgagcccct cacaccctgg atccttgacc tactaggcca ttatgctgtg atgaacaacc
      841 ccaccagaca gcctttggcc ctaaacgttg catacaggcg ctgcttgcag attctggctg
      901 caggactgtt cctgccaggt tcagtgggta tcactgaccc ctgtgagagt ggcaacttta
      961 gagtacacac agtcatgacc ctagaacagc aggacatggt ctgctataca gctcagactc
     1021 tcgtccgaat cctctcacat ggtggcttta ggaagatcct tggccaggag ggtgatgcca
     1081 gctatcttgc ttctgaaata tctacctggg atggagtgat agtaacacct tcagaaaagg
     1141 cttatgagaa gccaccagag aagaaggaag gagaggaaga agaggagaat acagaagaac
     1201 cacctcaagg agaggaagaa gaaagcatgg aaactcagga gtgacattcc cttcactcct
     1261 tttcctaccc aagggggaag actggagcct aagctgcctg ctactgggct ttacatggtg
     1321 acagacattt ccgtgggata gggaagatag caggaagaaa agtaaactcc atagaagtgt
     1381 cattccactg ggttttgata ttggcttagc tgccagtctc ccatttgtga cctatgccat
     1441 ccatctataa tggaggatac caacatttct tcctaatatt ctataatctc caactcctga
     1501 aaacccctct ctcaactaat actttgctgt tgaaatgttg tgaaatgtta agtgtctgga
     1561 aatttttttt tctaagaaaa actattaaag tacttcctag tagggctctt ggtctttctg
     1621 gttggtgttt tttgtttgtt tgtttttttg catgtggcaa ccttttttgc ttttggcttt
     1681 gtgtactctt ttgttgaaaa tgataattga gtcatttaat tataaatact cagatcacaa
     1741 tttgaagctg tctttgaatt aaaggacaaa ctcctggaac ctttccatat accagagtaa
     1801 actagaatat cattttaagt atttattgaa taaaatgatt ctatcaacac tg
//
