LOCUS       NM_002184               9027 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens interleukin 6 cytokine family signal transducer
            (IL6ST), transcript variant 1, mRNA.
ACCESSION   NM_002184
VERSION     NM_002184.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Osman,E.E.A. and Neamati,N.
  TITLE     Ironing Out the Mechanism of gp130 Signaling
  JOURNAL   Pharmacol Rev 76 (6), 1399-1443 (2024)
   PUBMED   39414364
  REMARK    GeneRIF: Ironing Out the Mechanism of gp130 Signaling.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9027)
  AUTHORS   Basu,S., Goel,S., Rawat,A., Vignesh,P. and Saikia,B.
  TITLE     An Indian Family with Autosomal Dominant Hyper-IgE Syndrome Due to
            IL6ST Defect
  JOURNAL   J Clin Immunol 44 (4), 90 (2024)
   PUBMED   38578568
  REMARK    GeneRIF: An Indian Family with Autosomal Dominant Hyper-IgE
            Syndrome Due to IL6ST Defect.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9027)
  AUTHORS   Shehata,R.R., Atta,S.A., Fatma,A.S., Aml,R.A. and Gomaa,A.S.
  TITLE     Association of serum IL-30 and soluble GP130 with the risk of
            psoriasis vulgaris
  JOURNAL   Egypt J Immunol 31 (2), 61-70 (2024)
   PUBMED   38615236
  REMARK    GeneRIF: Association of serum IL-30 and soluble GP130 with the risk
            of psoriasis vulgaris.
REFERENCE   4  (bases 1 to 9027)
  AUTHORS   Richards,P.J., Nowell,M.A., Horiuchi,S., McLoughlin,R.M.,
            Fielding,C.A., Grau,S., Yamamoto,N., Ehrmann,M., Rose-John,S.,
            Williams,A.S., Topley,N. and Jones,S.A.
  TITLE     Functional characterization of a soluble gp130 isoform and its
            therapeutic capacity in an experimental model of inflammatory
            arthritis
  JOURNAL   Arthritis Rheum 54 (5), 1662-1672 (2006)
   PUBMED   16646038
REFERENCE   5  (bases 1 to 9027)
  AUTHORS   Moritz,R.L., Hall,N.E., Connolly,L.M. and Simpson,R.J.
  TITLE     Determination of the disulfide structure and N-glycosylation sites
            of the extracellular domain of the human signal transducer gp130
  JOURNAL   J Biol Chem 276 (11), 8244-8253 (2001)
   PUBMED   11098061
REFERENCE   6  (bases 1 to 9027)
  AUTHORS   Tanaka,M., Kishimura,M., Ozaki,S., Osakada,F., Hashimoto,H.,
            Okubo,M., Murakami,M. and Nakao,K.
  TITLE     Cloning of novel soluble gp130 and detection of its neutralizing
            autoantibodies in rheumatoid arthritis
  JOURNAL   J Clin Invest 106 (1), 137-144 (2000)
   PUBMED   10880057
REFERENCE   7  (bases 1 to 9027)
  AUTHORS   Rodriguez,C., Grosgeorge,J., Nguyen,V.C., Gaudray,P. and
            Theillet,C.
  TITLE     Human gp130 transducer chain gene (IL6ST) is localized to
            chromosome band 5q11 and possesses a pseudogene on chromosome band
            17p11
  JOURNAL   Cytogenet Cell Genet 70 (1-2), 64-67 (1995)
   PUBMED   7736792
REFERENCE   8  (bases 1 to 9027)
  AUTHORS   Ip,N.Y., Nye,S.H., Boulton,T.G., Davis,S., Taga,T., Li,Y.,
            Birren,S.J., Yasukawa,K., Kishimoto,T., Anderson,D.J. et al.
  TITLE     CNTF and LIF act on neuronal cells via shared signaling pathways
            that involve the IL-6 signal transducing receptor component gp130
  JOURNAL   Cell 69 (7), 1121-1132 (1992)
   PUBMED   1617725
REFERENCE   9  (bases 1 to 9027)
  AUTHORS   Hibi,M., Murakami,M., Saito,M., Hirano,T., Taga,T. and Kishimoto,T.
  TITLE     Molecular cloning and expression of an IL-6 signal transducer,
            gp130
  JOURNAL   Cell 63 (6), 1149-1157 (1990)
   PUBMED   2261637
REFERENCE   10 (bases 1 to 9027)
  AUTHORS   Taga,T., Hibi,M., Hirata,Y., Yamasaki,K., Yasukawa,K., Matsuda,T.,
            Hirano,T. and Kishimoto,T.
  TITLE     Interleukin-6 triggers the association of its receptor with a
            possible signal transducer, gp130
  JOURNAL   Cell 58 (3), 573-581 (1989)
   PUBMED   2788034
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA863210.1, M57230.1,
            BC117402.1, AC016596.6, AC008914.7 and BQ013034.1.
            
            On Nov 22, 2018 this sequence version replaced NM_002184.3.
            
            Summary: The protein encoded by this gene is a signal transducer
            shared by many cytokines, including interleukin 6 (IL6), ciliary
            neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and
            oncostatin M (OSM). This protein functions as a part of the
            cytokine receptor complex. The activation of this protein is
            dependent upon the binding of cytokines to their receptors. vIL6, a
            protein related to IL6 and encoded by the Kaposi sarcoma-associated
            herpesvirus, can bypass the interleukin 6 receptor (IL6R) and
            directly activate this protein. Knockout studies in mice suggest
            that this gene plays a critical role in regulating myocyte
            apoptosis. Alternatively spliced transcript variants have been
            described. A related pseudogene has been identified on chromosome
            17. [provided by RefSeq, May 2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.3928977.1, BC071555.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in immune response or antiviral
                                      activity
            MANE Ensembl match     :: ENST00000381298.7/ ENSP00000370698.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                DA863210.1         31-58
            29-304              M57230.1           1-276
            305-724             BC117402.1         134-553
            725-3113            M57230.1           697-3085
            3114-3309           AC016596.6         69662-69857
            3310-9021           AC008914.7         133966-139677       c
            9022-9027           BQ013034.1         15-20               c
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q11.2"
     gene            1..9027
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /note="interleukin 6 cytokine family signal transducer"
                     /db_xref="GeneID:3572"
                     /db_xref="HGNC:HGNC:6021"
                     /db_xref="MIM:600694"
     exon            1..180
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            181..268
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            269..347
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     CDS             284..3040
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; membrane glycoprotein gp130; CD130 antigen;
                     interleukin receptor beta chain; gp130 of the rheumatoid
                     arthritis antigenic peptide-bearing soluble form; IL-6R
                     subunit beta; membrane glycoprotein 130; IL-6 receptor
                     subunit beta; oncostatin-M receptor subunit alpha;
                     interleukin-6 signal transducer; gp130, oncostatin M
                     receptor"
                     /codon_start=1
                     /product="interleukin-6 receptor subunit beta isoform 1
                     precursor"
                     /protein_id="NP_002175.2"
                     /db_xref="CCDS:CCDS3971.1"
                     /db_xref="GeneID:3572"
                     /db_xref="HGNC:HGNC:6021"
                     /db_xref="MIM:600694"
                     /translation="MLTLQTWLVQALFIFLTTESTGELLDPCGYISPESPVVQLHSNF
                     TAVCVLKEKCMDYFHVNANYIVWKTNHFTIPKEQYTIINRTASSVTFTDIASLNIQLT
                     CNILTFGQLEQNVYGITIISGLPPEKPKNLSCIVNEGKKMRCEWDGGRETHLETNFTL
                     KSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAENALGKVTSDHINFDPVY
                     KVKPNPPHNLSVINSEELSSILKLTWTNPSIKSVIILKYNIQYRTKDASTWSQIPPED
                     TASTRSSFTVQDLKPFTEYVFRIRCMKEDGKGYWSDWSEEASGITYEDRPSKAPSFWY
                     KIDPSHTQGYRTVQLVWKTLPPFEANGKILDYEVTLTRWKSHLQNYTVNATKLTVNLT
                     NDRYLATLTVRNLVGKSDAAVLTIPACDFQATHPVMDLKAFPKDNMLWVEWTTPRESV
                     KKYILEWCVLSDKAPCITDWQQEDGTVHRTYLRGNLAESKCYLITVTPVYADGPGSPE
                     SIKAYLKQAPPSKGPTVRTKKVGKNEAVLEWDQLPVDVQNGFIRNYTIFYRTIIGNET
                     AVNVDSSHTEYTLSSLTSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEIEAIVVP
                     VCLAFLLTTLLGVLFCFNKRDLIKKHIWPNVPDPSKSHIAQWSPHTPPRHNFNSKDQM
                     YSDGNFTDVSVVEIEANDKKPFPEDLKSLDLFKKEKINTEGHSSGIGGSSCMSSSRPS
                     ISSSDENESSQNTSSTVQYSTVVHSGYRHQVPSVQVFSRSESTQPLLDSEERPEDLQL
                     VDHVDGGDGILPRQQYFKQNCSQHESSPDISHFERSKQVSSVNEEDFVRLKQQISDHI
                     SQSCGSGQMKMFQEVSAADAFGPGTEGQVERFETVGMEAATDEGMPKSYLPQTVRQGG
                     YMPQ"
     exon            348..653
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            654..774
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            775..941
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1096
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1256
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1339
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1550
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1733
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1835
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1982
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2123
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2124..2220
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2221..2302
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2303..9027
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7455..7460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /note="hexamer: AATAAA"
     polyA_site      7476
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /note="major polyA site"
     regulatory      9003..9008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
                     /note="hexamer: AATAAA"
     polyA_site      9027
                     /gene="IL6ST"
                     /gene_synonym="CD130; CDW130; GP130; HIES4; HIES4A;
                     HIES4B; IL-6RB; IMD94; sGP130; STWS2"
ORIGIN      
        1 ggatctgaca gtgttccgga gccggggcga gcagccaaaa ggcccgcgga gtcgcgctgg
       61 gccgccccgg cgcagctgaa ccgggggccg cgcctgccag gccgacgggt ctggcccagc
      121 ctggcgccaa ggggttcgtg cgctgtggag acgcggaggg tcgaggcggc gcggcctgag
      181 tgaaacccaa tggaaaaagc atgacattta gaagtagaag acttagcttc aaatccctac
      241 tccttcactt actaattttg tgatttggaa atatccgcgc aagatgttga cgttgcagac
      301 ttggctagtg caagccttgt ttattttcct caccactgaa tctacaggtg aacttctaga
      361 tccatgtggt tatatcagtc ctgaatctcc agttgtacaa cttcattcta atttcactgc
      421 agtttgtgtg ctaaaggaaa aatgtatgga ttattttcat gtaaatgcta attacattgt
      481 ctggaaaaca aaccatttta ctattcctaa ggagcaatat actatcataa acagaacagc
      541 atccagtgtc acctttacag atatagcttc attaaatatt cagctcactt gcaacattct
      601 tacattcgga cagcttgaac agaatgttta tggaatcaca ataatttcag gcttgcctcc
      661 agaaaaacct aaaaatttga gttgcattgt gaacgagggg aagaaaatga ggtgtgagtg
      721 ggatggtgga agggaaacac acttggagac aaacttcact ttaaaatctg aatgggcaac
      781 acacaagttt gctgattgca aagcaaaacg tgacaccccc acctcatgca ctgttgatta
      841 ttctactgtg tattttgtca acattgaagt ctgggtagaa gcagagaatg cccttgggaa
      901 ggttacatca gatcatatca attttgatcc tgtatataaa gtgaagccca atccgccaca
      961 taatttatca gtgatcaact cagaggaact gtctagtatc ttaaaattga catggaccaa
     1021 cccaagtatt aagagtgtta taatactaaa atataacatt caatatagga ccaaagatgc
     1081 ctcaacttgg agccagattc ctcctgaaga cacagcatcc acccgatctt cattcactgt
     1141 ccaagacctt aaacctttta cagaatatgt gtttaggatt cgctgtatga aggaagatgg
     1201 taagggatac tggagtgact ggagtgaaga agcaagtggg atcacctatg aagatagacc
     1261 atctaaagca ccaagtttct ggtataaaat agatccatcc catactcaag gctacagaac
     1321 tgtacaactc gtgtggaaga cattgcctcc ttttgaagcc aatggaaaaa tcttggatta
     1381 tgaagtgact ctcacaagat ggaaatcaca tttacaaaat tacacagtta atgccacaaa
     1441 actgacagta aatctcacaa atgatcgcta tctagcaacc ctaacagtaa gaaatcttgt
     1501 tggcaaatca gatgcagctg ttttaactat ccctgcctgt gactttcaag ctactcaccc
     1561 tgtaatggat cttaaagcat tccccaaaga taacatgctt tgggtggaat ggactactcc
     1621 aagggaatct gtaaagaaat atatacttga gtggtgtgtg ttatcagata aagcaccctg
     1681 tatcacagac tggcaacaag aagatggtac cgtgcatcgc acctatttaa gagggaactt
     1741 agcagagagc aaatgctatt tgataacagt tactccagta tatgctgatg gaccaggaag
     1801 ccctgaatcc ataaaggcat accttaaaca agctccacct tccaaaggac ctactgttcg
     1861 gacaaaaaaa gtagggaaaa acgaagctgt cttagagtgg gaccaacttc ctgttgatgt
     1921 tcagaatgga tttatcagaa attatactat attttataga accatcattg gaaatgaaac
     1981 tgctgtgaat gtggattctt cccacacaga atatacattg tcctctttga ctagtgacac
     2041 attgtacatg gtacgaatgg cagcatacac agatgaaggt gggaaggatg gtccagaatt
     2101 cacttttact accccaaagt ttgctcaagg agaaattgaa gccatagtcg tgcctgtttg
     2161 cttagcattc ctattgacaa ctcttctggg agtgctgttc tgctttaata agcgagacct
     2221 aattaaaaaa cacatctggc ctaatgttcc agatccttca aagagtcata ttgcccagtg
     2281 gtcacctcac actcctccaa ggcacaattt taattcaaaa gatcaaatgt attcagatgg
     2341 caatttcact gatgtaagtg ttgtggaaat agaagcaaat gacaaaaagc cttttccaga
     2401 agatctgaaa tcattggacc tgttcaaaaa ggaaaaaatt aatactgaag gacacagcag
     2461 tggtattggg gggtcttcat gcatgtcatc ttctaggcca agcatttcta gcagtgatga
     2521 aaatgaatct tcacaaaaca cttcgagcac tgtccagtat tctaccgtgg tacacagtgg
     2581 ctacagacac caagttccgt cagtccaagt cttctcaaga tccgagtcta cccagccctt
     2641 gttagattca gaggagcggc cagaagatct acaattagta gatcatgtag atggcggtga
     2701 tggtattttg cccaggcaac agtacttcaa acagaactgc agtcagcatg aatccagtcc
     2761 agatatttca cattttgaaa ggtcaaagca agtttcatca gtcaatgagg aagattttgt
     2821 tagacttaaa cagcagattt cagatcatat ttcacaatcc tgtggatctg ggcaaatgaa
     2881 aatgtttcag gaagtttctg cagcagatgc ttttggtcca ggtactgagg gacaagtaga
     2941 aagatttgaa acagttggca tggaggctgc gactgatgaa ggcatgccta aaagttactt
     3001 accacagact gtacggcaag gcggctacat gcctcagtga aggactagta gttcctgcta
     3061 caacttcagc agtacctata aagtaaagct aaaatgattt tatctgtgaa ttcagatttt
     3121 aaaaagtctt cactctctga agatgatcat ttgcccttaa ggacaaaaat gaactgaagt
     3181 ttcacatgag ctatttccat tccagaatat ctgggattct actttaagca ctacataaac
     3241 tgactttatc ctcagactag ctgaatgatt ttgtgctgtt tcaggatgtt tgcactgaag
     3301 aaaaacagaa agcttatctg aaatttataa aactttttgt tttgctacat agaaaacaga
     3361 aggtatttga ataataagca gtgatatgct tagtgagcac agctatactg attttgatta
     3421 gaatagtcat cagagtggct tagggacagt taatataaaa gaggagcaag gtgtagacca
     3481 tcatctactt ctgctaaaat aacttaaaaa gaggtccata ggccataact acatgagccc
     3541 agcttttgta atctgacaaa aaaatgagga gcagcttcgt gtatatcagt gtacacggta
     3601 ttccttaggt cccttccatt ggtagtgatg ctgcgagtta ttactggaga aaaggaattc
     3661 tagagcttta acttggcaga ttaaaagtac tcatttttta ttcatcaata attagtaatc
     3721 tcactagttt tcaaaaattt gcatattatt gacaacctct ttgaagatgc atttcacaaa
     3781 ctcaacagag tgccatgata agagctaggg atcccccaaa ctatctcaag catctaaaaa
     3841 attgccattt ttaaaggctt aaattgtagt agtaaagggg aaaacaggaa gtagtagtaa
     3901 aggggaaaaa aaaccaataa agcatctaaa aaattggcat gttaaaaggc ttaaattgct
     3961 aatgtgtgta tatatatata tatatataca cacacatatc attgactttt cttaagactt
     4021 cagagtactg ggtagatgaa cactttatac agtatatatc ttcagcttaa atttgttttg
     4081 agtatttttt ttatttttaa ataagtaggc aaagatttaa atttttttat ttttagtaaa
     4141 tgtttgaggc acactaagac aacttgggca atatttgcca aaacaaaaca gaaccccaaa
     4201 aaatgtacat cttgttctta gcaaatatca ttattgtaga gacacttaat aaagagatgg
     4261 tattttaatg tctgcagttc tgaggtaggg tggaacttag ttctacattg tgatttagga
     4321 atttttaaaa ccttttttct tcaagggaga agtgacccag gcctcgagtt tagtgctaaa
     4381 gccgctagtg tacttatgct gtcccctaac caccacgtgc gatatggaag cagatgctaa
     4441 atataggggt tttcttagaa agtaagagga aattagcaag cgttattagt gattgactac
     4501 tgctatcaag tgaattcaaa ggaaacaggt ttttatgcca tatttaagtt acagaaacca
     4561 ggcatgctta gaatagtttc tagaggttat tggagaatag aaagctaaga aaacttggta
     4621 tacatttaca atggaaatat aattacactt tttactctca gaatattgtt cacattagac
     4681 ttcctgttta tcttttatat tcttgcattt atataatgcc tcatcctttc aaagttcttt
     4741 cacatattat atgatcttct ttatgaaaaa aatagatgtt tcattctgat atattcagtt
     4801 tcccacttta ggcaaaagta gattaataga atgacgaatt caaagtagat gaggaaaatc
     4861 aggcacagag aagtaaaggt agggatagac ccaaatttac acaacaagat aatgacatct
     4921 ccagctttta agttgatcat caaaggctgg gctggatttg tcttgctgta tgtgtcagga
     4981 aatttatacc tattacattt tccattttct caaaatttaa gtcacatgac taatatttag
     5041 ctgcaacttt cctcataaca aatagtgtca tgaagaatgt tgtagtgtga agtttgtaca
     5101 tttcagggtc agatatacaa tatgaactct taatctacag gaatgagaat ggaggatcat
     5161 tgaaggccat gatataaaca aatttgcatg ttgaagcctg tataaaacat ggtacagtga
     5221 gtgaatatac ccccatcccc aagaacactt tatacatatt aaatggatat atgattactg
     5281 tgcaaaaatt cattctggaa atgaacatat atttgagcac taatatgtaa tgtacacctg
     5341 ccctaaggag aaaataaatt ataaaacttt ttacattcaa aattactttc ccaagcatgt
     5401 cttagaataa tctatgtgtt gatgcatgta aattgtactt taggtaggca aagaaatctg
     5461 gttatttatg taaaaactag tctaataaag ttagttagtg gctttatcac tttaaatctt
     5521 tagtgtccaa aagtggtgtt taaagtaata gcacatcaga aaaccttgtc tggacaaaac
     5581 tagttcactc actgcttctg cacctgcagt tgctcccttt agggttataa aataatgacc
     5641 caaatgttac atgtgttgat attataactt gtcagttact gatgtctgtg gtatcctacc
     5701 ctcatctctg aaagggataa tactgaataa ttattagaaa actataaaac ttcacacttt
     5761 gtaccattaa aacctaaaat tttaatcttg tcctttttta ctatggatca gtcggcactc
     5821 gggaacagca gcaaggaaaa aaagcaaatt tcattcacat gttctgtgtt catacctctt
     5881 ctctacctaa ttgttcattt aaatttcagc cttattcctt gataagggat tttaccacat
     5941 gaagtcatcc agtgacccta gctcttattg tgaagttagt ggagtatact tagaaatgtt
     6001 acaactttaa aatgttacaa aacattcatt aaagctcata tttaaagtag agcatctagt
     6061 ttgagaaata gaaatcaatt attaaagatg tcttttttct acccatttaa ctagttaaaa
     6121 ccatgacatg taaatgtaga agtagaataa tcatagaatt ccctaaaata tttctgttta
     6181 ctaacatata ttgaccaagt acatcaagca ggagagatct tccttcattc tgttatagtc
     6241 cacatcattc taattttgct cagttgttat taagagcata ttcctaaacc atacactttt
     6301 gtttcaataa agttttattt tgttgagatg aataaaataa caaagttata agctgcataa
     6361 gacaaaagtt caattgttca aaaaaaattt actgggatag ctttctatta caggtattgt
     6421 tagattatat tgtgctgata agattacttt ctaaaaaatt tgtacttttc tgtaaattaa
     6481 aagaatatgg agtcataaaa tggcaagtgt tttaggatta gcctaaaatt ggacattgtc
     6541 attgatttca aagaaggtat gaactagcag tcttacagcc taattcttct ttggactggt
     6601 ccttggcagc agttcctttt cagactcgat aaacagaatt cagatgatgt aagtcaaaac
     6661 aaaactttac aaagccaagc gtattatctt ttgcattaac ctattttttt ccatcataca
     6721 tgctactagt atgtgcatta gcatgatatt ctcatataca ttgcattaaa aattaaaagg
     6781 tggcagctca gggtgagctc ttctgttgct catttgttcc taaattttta agggcttttt
     6841 ctcagtcaat agtttgtaca aactggttag tttaacttca ttacccattt cattaaagtt
     6901 gatgggtcgt gtgatgagat gcatttaagg ccgatagtga tagatgtttt ttttatttct
     6961 tgaacacagg ctttgtctga atgatgttct tttatctctt gaacacaagc tttgaatgat
     7021 aactacaggt tttaagtgct gttacattaa taccataatg tgatgtgtta gaaacaaagg
     7081 gatatttcaa aggtagatat ttgaaaattc tctagtctca atatgtatgt gtattgaata
     7141 tactctaaaa ataaatgtgc aatttgctag taggacaatg cagtgactga ctagcattag
     7201 gtatgtttct tttatatcct agctatgtcc cactttcttc taagtgcaat cctttcatgt
     7261 tcacttgctg ttttacccca tctactctaa cttcatttgg aaggcttgtc tagagtatag
     7321 catgtatttt tacctttgca gtgaattgca tgtgctaatt gtaaccacag ctatttttat
     7381 gttgacataa ctccaaatgt tatattaaat gttctattat atattagctc taatccctta
     7441 agtaaatttt aagaaataaa tacttgttca aatttttttt ctgtatgtgg ttactatcat
     7501 ctgactatgc atatttgtaa cagcatttat cattagtggt gttagctaaa taagcatctt
     7561 agtgtaaatg agatgcttcg tgtgggtttt gtgacatttt aaatgacata atggaatgtg
     7621 atttaaaaga aaaccagtac actatcttgg tcttaataac atagaatgga gatggcaaat
     7681 ttatccacta gttttccaga tttactattt aatagctgag gtctgaaatc gtagcatcct
     7741 ccctcctagt ggacattaaa aaaaaaaaaa aaaaaaaaaa cctacttggt tgtcaagagc
     7801 ccaagtatgg aggtgctgcg ccatcttgtg gcctgtctgt gcccaccctg cactctgctg
     7861 gagtctccat ccttgttgca gtgagacttg aagttcaaga ttgatacatg gcatcctcct
     7921 gctacttctt gaggttacta agtagtatat gaaactaatc agtcagcaag tccacctgga
     7981 aggaaaagaa aatctcaact attaatgtgc cttcacattg tgattttgtc taaaaaaatg
     8041 tagtgagtca aaaaacccac aagccagcca acagtaactc cttcacatat ataccagagt
     8101 ttatagaaat aacatgtcag ctttgggcta tgtgctcctt tgtttaaaat cttctatttg
     8161 gttatggctt gtataggctc aagcctgatt tctttaaggt gtggtggctc atcttatcct
     8221 aatgtgtatg atagatacag tccatcctgc tttggaaaag attatgtaac tccttgagag
     8281 catactcttt ctctagccca aaggcagtga gagagttttc ttgttcagga ttgcttaact
     8341 ttccatttaa gctttttctt tttaaattaa tacaaacttc tacactttca aaatacgaaa
     8401 tatattacaa ctgcgtatag gctcttccat acttaagtcc agtgcttggg caagttaatg
     8461 gagtgaaaga ctacaagcaa agaggaactg aggtagaaaa agaagaatgt gtgaaagcag
     8521 caggaagctc agccaactcg aaagcagggt gaacagcttg agtcctgttg ctgctgatcg
     8581 gggttggctc ttggacaact tagtaagatc atggaaaggc tgcttgggtt ctccatagaa
     8641 aagttctgtc tccatcaagg gaggaaaatg tacctttcaa ctcaaaattc aatatttgtt
     8701 tttaaatata gctattttcc ccaaccgcta aagattttca acagatacga agccagagct
     8761 tagttttaga aacctgtgga cattcaaacc tgattcttta ttccctgtga ctatggttat
     8821 gtcattttac atgtcaaaaa agtgtatcta gaattgtcat ttcttatttt tgagcttttt
     8881 ttagtgagaa ttatcccctc acttaaatgg ctttttattt aaacatctgt gcattctgta
     8941 tgaaattgta gtctttctgg gataacatgg tgagctatat ggtggtaatc cacacacaca
     9001 aaaataaaag ccaaaaaaaa accaaaa
//
