LOCUS       NM_001376923             818 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens interleukin 32 (IL32), transcript variant 19, mRNA.
ACCESSION   NM_001376923
VERSION     NM_001376923.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Li,P., Que,Y., Wong,C., Lin,Y., Qiu,J., Gao,B., Zhou,H., Hu,W.,
            Shi,H., Peng,Y., Huang,D., Gao,W., Qiu,X. and Liang,A.
  TITLE     IL-32 aggravates metabolic disturbance in human nucleus pulposus
            cells by activating FAT4-mediated Hippo/YAP signaling
  JOURNAL   Int Immunopharmacol 141, 112966 (2024)
   PUBMED   39178518
  REMARK    GeneRIF: IL-32 aggravates metabolic disturbance in human nucleus
            pulposus cells by activating FAT4-mediated Hippo/YAP signaling.
            Erratum:[Int Immunopharmacol. 2024 Dec 5;142(Pt A):113049. doi:
            10.1016/j.intimp.2024.113049.. PMID: 39294029]
REFERENCE   2  (bases 1 to 818)
  AUTHORS   Liu,J., Li,W., Wang,J., Bai,L., Xu,J., Chen,X., Wang,S., Li,L. and
            Xu,X.
  TITLE     IL-32 regulates trophoblast invasion through
            miR-205-NFkappaB-MMP2/9 axis contributing to the pregnancy-induced
            hypertensiondagger
  JOURNAL   Biol Reprod 111 (4), 780-799 (2024)
   PUBMED   39101465
  REMARK    GeneRIF: IL-32 regulates trophoblast invasion through
            miR-205-NFkappaB-MMP2/9 axis contributing to the pregnancy-induced
            hypertensiondagger.
REFERENCE   3  (bases 1 to 818)
  AUTHORS   Leng,K., Rooney,B., McCarthy,F., Xia,W., Rose,I.V.L., Bax,S.,
            Chin,M., Fathi,S., Herrington,K.A., Leonetti,M., Kao,A.,
            Fancy,S.P.J., Elias,J.E. and Kampmann,M.
  TITLE     mTOR activation induces endolysosomal remodeling and nonclassical
            secretion of IL-32 via exosomes in inflammatory reactive astrocytes
  JOURNAL   J Neuroinflammation 21 (1), 198 (2024)
   PUBMED   39118084
  REMARK    GeneRIF: mTOR activation induces endolysosomal remodeling and
            nonclassical secretion of IL-32 via exosomes in inflammatory
            reactive astrocytes.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 818)
  AUTHORS   Zhao,S., Li,L., Komohara,Y., Matsubara,E., Shinchi,Y., Adawy,A.,
            Yano,H., Pan,C., Fujiwara,Y., Ikeda,K., Suzu,S., Hibi,T. and
            Suzuki,M.
  TITLE     IL-32 production from lung adenocarcinoma cells is potentially
            involved in immunosuppressive microenvironment
  JOURNAL   Med Mol Morphol 57 (2), 91-100 (2024)
   PUBMED   38316697
  REMARK    GeneRIF: IL-32 production from lung adenocarcinoma cells is
            potentially involved in immunosuppressive microenvironment.
REFERENCE   5  (bases 1 to 818)
  AUTHORS   Sasidharan,K., Caddeo,A., Jamialahmadi,O., Noto,F.R., Tomasi,M.,
            Malvestiti,F., Ciociola,E., Tavaglione,F., Mancina,R.M.,
            Cherubini,A., Bianco,C., Mirarchi,A., Mannisto,V., Pihlajamaki,J.,
            Karja,V., Grimaudo,S., Luukkonen,P.K., Qadri,S., Yki-Jarvinen,H.,
            Petta,S., Manfrini,S., Vespasiani-Gentilucci,U., Bruni,V.,
            Valenti,L. and Romeo,S.
  TITLE     IL32 downregulation lowers triglycerides and type I collagen in
            di-lineage human primary liver organoids
  JOURNAL   Cell Rep Med 5 (1), 101352 (2024)
   PUBMED   38232700
  REMARK    GeneRIF: IL32 downregulation lowers triglycerides and type I
            collagen in di-lineage human primary liver organoids.
REFERENCE   6  (bases 1 to 818)
  AUTHORS   Goda,C., Kanaji,T., Kanaji,S., Tanaka,G., Arima,K., Ohno,S. and
            Izuhara,K.
  TITLE     Involvement of IL-32 in activation-induced cell death in T cells
  JOURNAL   Int Immunol 18 (2), 233-240 (2006)
   PUBMED   16410314
  REMARK    GeneRIF: results strongly indicate that IL-32 is involved in
            activation-induced cell death in T cells, probably via its
            intracellular actions
REFERENCE   7  (bases 1 to 818)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   8  (bases 1 to 818)
  AUTHORS   Kim,S.H., Han,S.Y., Azam,T., Yoon,D.Y. and Dinarello,C.A.
  TITLE     Interleukin-32: a cytokine and inducer of TNFalpha
  JOURNAL   Immunity 22 (1), 131-142 (2005)
   PUBMED   15664165
REFERENCE   9  (bases 1 to 818)
  AUTHORS   Bernot,A., Heilig,R., Clepet,C., Smaoui,N., Da Silva,C.,
            Petit,J.L., Devaud,C., Chiannilkulchai,N., Fizames,C., Samson,D.,
            Cruaud,C., Caloustian,C., Gyapay,G., Delpech,M. and Weissenbach,J.
  TITLE     A transcriptional Map of the FMF region
  JOURNAL   Genomics 50 (2), 147-160 (1998)
   PUBMED   9653642
REFERENCE   10 (bases 1 to 818)
  AUTHORS   Dahl,C.A., Schall,R.P., He,H.L. and Cairns,J.S.
  TITLE     Identification of a novel gene expressed in activated natural
            killer cells and T cells
  JOURNAL   J Immunol 148 (2), 597-603 (1992)
   PUBMED   1729377
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC108134.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: This gene encodes a member of the cytokine family. The
            protein contains a tyrosine sulfation site, 3 potential
            N-myristoylation sites, multiple putative phosphorylation sites,
            and an RGD cell-attachment sequence. Expression of this protein is
            increased after the activation of T-cells by mitogens or the
            activation of NK cells by IL-2. This protein induces the production
            of TNFalpha from macrophage cells. Alternate transcriptional splice
            variants, encoding different isoforms, have been characterized.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC105602.1, BP331861.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000525643.7/ ENSP00000432218.3
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AC108134.3         77164-77211
            49-91               AC108134.3         77308-77350
            92-130              AC108134.3         78901-78939
            131-190             AC108134.3         79078-79137
            191-217             AC108134.3         79508-79534
            218-277             AC108134.3         79704-79763
            278-818             AC108134.3         80514-81054
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..818
                     /gene="IL32"
                     /gene_synonym="IL-32alpha; IL-32beta; IL-32delta;
                     IL-32gamma; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd"
                     /note="interleukin 32"
                     /db_xref="GeneID:9235"
                     /db_xref="HGNC:HGNC:16830"
                     /db_xref="MIM:606001"
     exon            1..48
                     /gene="IL32"
                     /gene_synonym="IL-32alpha; IL-32beta; IL-32delta;
                     IL-32gamma; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd"
                     /inference="alignment:Splign:2.1.0"
     exon            49..91
                     /gene="IL32"
                     /gene_synonym="IL-32alpha; IL-32beta; IL-32delta;
                     IL-32gamma; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..643
                     /gene="IL32"
                     /gene_synonym="IL-32alpha; IL-32beta; IL-32delta;
                     IL-32gamma; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd"
                     /note="isoform B is encoded by transcript variant 19;
                     natural killer cell transcript 4; natural killer cells
                     protein 4; tumor necrosis factor alpha-inducing factor;
                     interleukin-32 theta; interleukin-32 small; interleukin-32
                     eta"
                     /codon_start=1
                     /product="interleukin-32 isoform B"
                     /protein_id="NP_001363852.1"
                     /db_xref="CCDS:CCDS32377.1"
                     /db_xref="GeneID:9235"
                     /db_xref="HGNC:HGNC:16830"
                     /db_xref="MIM:606001"
                     /translation="MCFPKVLSDDMKKLKARMHQAIERFYDKMQNAESGRGQVMSSLA
                     ELEDDFKEGYLETVAAYYEEQHPELTPLLEKERDGLRCRGNRSPVPDVEDPATEEPGE
                     SFCDKVMRWFQAMLQRLQTWWHGVLAWVKEKVVALVHAVQALWKQFQSFCCSLSELFM
                     SSFQSYGAPRGDKEELTPQKCSEPQSSK"
     exon            92..130
                     /gene="IL32"
                     /gene_synonym="IL-32alpha; IL-32beta; IL-32delta;
                     IL-32gamma; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd"
                     /inference="alignment:Splign:2.1.0"
     exon            131..190
                     /gene="IL32"
                     /gene_synonym="IL-32alpha; IL-32beta; IL-32delta;
                     IL-32gamma; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd"
                     /inference="alignment:Splign:2.1.0"
     exon            191..217
                     /gene="IL32"
                     /gene_synonym="IL-32alpha; IL-32beta; IL-32delta;
                     IL-32gamma; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd"
                     /inference="alignment:Splign:2.1.0"
     exon            218..277
                     /gene="IL32"
                     /gene_synonym="IL-32alpha; IL-32beta; IL-32delta;
                     IL-32gamma; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd"
                     /inference="alignment:Splign:2.1.0"
     exon            278..818
                     /gene="IL32"
                     /gene_synonym="IL-32alpha; IL-32beta; IL-32delta;
                     IL-32gamma; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd"
                     /inference="alignment:Splign:2.1.0"
     regulatory      797..802
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IL32"
                     /gene_synonym="IL-32alpha; IL-32beta; IL-32delta;
                     IL-32gamma; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd"
                     /note="hexamer: AATAAA"
     polyA_site      818
                     /gene="IL32"
                     /gene_synonym="IL-32alpha; IL-32beta; IL-32delta;
                     IL-32gamma; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd"
                     /note="major polyA site"
ORIGIN      
        1 agactgcgtg cagaaggtga ctgtctcagt ggagctgggt catctcaggc cttggctcct
       61 tgaacttttg gccgccatgt gcttcccgaa ggtcctctct gatgacatga agaagctgaa
      121 ggcccgaatg caccaggcca tagaaagatt ttatgataaa atgcaaaatg cagaatcagg
      181 acgtggacag gtgatgtcga gcctggcaga gctggaggac gacttcaaag agggctacct
      241 ggagacagtg gcggcttatt atgaggagca gcacccagag ctcactcctc tacttgaaaa
      301 agaaagagat ggattacggt gccgaggcaa cagatcccct gtcccggatg ttgaggatcc
      361 cgcaaccgag gagcctgggg agagcttttg tgacaaggtc atgagatggt tccaggccat
      421 gctgcagcgg ctgcagacct ggtggcacgg ggttctggcc tgggtgaagg agaaggtggt
      481 ggccctggtc catgcagtgc aggccctctg gaaacagttc cagagtttct gctgctctct
      541 gtcagagctc ttcatgtcct ctttccagtc ctacggagcc ccacgggggg acaaggagga
      601 gctgacaccc cagaagtgct ctgaacccca atcctcaaaa tgaagatact gacaccacct
      661 ttgccctccc cgtcaccgcg cacccaccct gacccctccc tcagctgtcc tgtgccccgc
      721 cctctcccgc acactcagtc cccctgcctg gcgttcctgc cgcagctctg acctggtgct
      781 gtcgccctgg catcttaata aaacctgctt atacttcc
//
