LOCUS       NM_002187               2364 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens interleukin 12B (IL12B), mRNA.
ACCESSION   NM_002187
VERSION     NM_002187.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Tishe,Z.H., Shawkat,S., Popy,M.N., Ahmed,A., Mumu,S.B., Apu,M.N.H.
            and Mostaid,M.S.
  TITLE     Interleukin gene polymorphisms and alopecia areata: A systematic
            review and meta-analysis
  JOURNAL   Medicine (Baltimore) 103 (8), e37300 (2024)
   PUBMED   38394507
  REMARK    GeneRIF: Interleukin gene polymorphisms and alopecia areata: A
            systematic review and meta-analysis.
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Landoni,E., Woodcock,M.G., Barragan,G., Casirati,G., Cinella,V.,
            Stucchi,S., Flick,L.M., Withers,T.A., Hudson,H., Casorati,G.,
            Dellabona,P., Genovese,P., Savoldo,B., Metelitsa,L.S. and Dotti,G.
  TITLE     IL-12 reprograms CAR-expressing natural killer T cells to
            long-lived Th1-polarized cells with potent antitumor activity
  JOURNAL   Nat Commun 15 (1), 89 (2024)
   PUBMED   38167707
  REMARK    GeneRIF: IL-12 reprograms CAR-expressing natural killer T cells to
            long-lived Th1-polarized cells with potent antitumor activity.
            Erratum:[Nat Commun. 2024 Jun 18;15(1):5182. doi:
            10.1038/s41467-024-49582-6.. PMID: 38890345]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2364)
  AUTHORS   Nyangiri,O.A., Mulindwa,J., Namulondo,J., Kitibwa,A., Nassuuna,J.,
            Elliott,A., Kimuda,M.P., Boobo,A., Nerima,B., Adriko,M.,
            Dunton,N.J., Madhan,G.K., Kristiansen,M., Casacuberta-Partal,M.,
            Noyes,H. and Matovu,E.
  CONSRTM   TrypanoGEN+ Research group of the H3Africa consortium
  TITLE     Variants of IL6, IL10, FCN2, RNASE3, IL12B and IL17B loci are
            associated with Schistosoma mansoni worm burden in the Albert Nile
            region of Uganda
  JOURNAL   PLoS Negl Trop Dis 17 (11), e0011796 (2023)
   PUBMED   38033168
  REMARK    GeneRIF: Variants of IL6, IL10, FCN2, RNASE3, IL12B and IL17B loci
            are associated with Schistosoma mansoni worm burden in the Albert
            Nile region of Uganda.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2364)
  AUTHORS   Zhang,M., Peng,L., Li,W., Duan,Y., Liu,X., Chen,S., Deng,J. and
            Liu,X.
  TITLE     IL12B and IL17 genes polymorphisms associated with differential
            susceptibility to juvenile idiopathic arthritis and juvenile-onset
            systemic lupus erythematosus in Chinese children
  JOURNAL   Medicine (Baltimore) 102 (31), e34477 (2023)
   PUBMED   37543802
  REMARK    GeneRIF: IL12B and IL17 genes polymorphisms associated with
            differential susceptibility to juvenile idiopathic arthritis and
            juvenile-onset systemic lupus erythematosus in Chinese children.
REFERENCE   5  (bases 1 to 2364)
  AUTHORS   Wang,P. and Liao,J.
  TITLE     Association between interleukin-12 +1188A/C polymorphism and the
            risk of tuberculosis: A meta-analysis
  JOURNAL   Afr Health Sci 23 (2), 133-138 (2023)
   PUBMED   38223634
  REMARK    GeneRIF: Association between interleukin-12 +1188A/C polymorphism
            and the risk of tuberculosis: A meta-analysis.
REFERENCE   6  (bases 1 to 2364)
  AUTHORS   Sieburth,D., Jabs,E.W., Warrington,J.A., Li,X., Lasota,J.,
            LaForgia,S., Kelleher,K., Huebner,K., Wasmuth,J.J. and Wolf,S.F.
  TITLE     Assignment of genes encoding a unique cytokine (IL12) composed of
            two unrelated subunits to chromosomes 3 and 5
  JOURNAL   Genomics 14 (1), 59-62 (1992)
   PUBMED   1358798
REFERENCE   7  (bases 1 to 2364)
  AUTHORS   Gearing,D.P. and Cosman,D.
  TITLE     Homology of the p40 subunit of natural killer cell stimulatory
            factor (NKSF) with the extracellular domain of the interleukin-6
            receptor
  JOURNAL   Cell 66 (1), 9-10 (1991)
   PUBMED   2070420
REFERENCE   8  (bases 1 to 2364)
  AUTHORS   Gubler,U., Chua,A.O., Schoenhaut,D.S., Dwyer,C.M., McComas,W.,
            Motyka,R., Nabavi,N., Wolitzky,A.G., Quinn,P.M., Familletti,P.C. et
            al.
  TITLE     Coexpression of two distinct genes is required to generate secreted
            bioactive cytotoxic lymphocyte maturation factor
  JOURNAL   Proc Natl Acad Sci U S A 88 (10), 4143-4147 (1991)
   PUBMED   1674604
REFERENCE   9  (bases 1 to 2364)
  AUTHORS   Wolf,S.F., Temple,P.A., Kobayashi,M., Young,D., Dicig,M., Lowe,L.,
            Dzialo,R., Fitz,L., Ferenz,C., Hewick,R.M. et al.
  TITLE     Cloning of cDNA for natural killer cell stimulatory factor, a
            heterodimeric cytokine with multiple biologic effects on T and
            natural killer cells
  JOURNAL   J Immunol 146 (9), 3074-3081 (1991)
   PUBMED   1673147
REFERENCE   10 (bases 1 to 2364)
  AUTHORS   Stern,A.S., Podlaski,F.J., Hulmes,J.D., Pan,Y.C., Quinn,P.M.,
            Wolitzky,A.G., Familletti,P.C., Stremlo,D.L., Truitt,T.,
            Chizzonite,R. et al.
  TITLE     Purification to homogeneity and partial characterization of
            cytotoxic lymphocyte maturation factor from human B-lymphoblastoid
            cells
  JOURNAL   Proc Natl Acad Sci U S A 87 (17), 6808-6812 (1990)
   PUBMED   2204066
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011418.5, M65272.1 and
            M65290.1.
            
            On Aug 31, 2019 this sequence version replaced NM_002187.2.
            
            Summary: This gene encodes a subunit of interleukin 12, a cytokine
            that acts on T and natural killer cells, and has a broad array of
            biological activities. Interleukin 12 is a disulfide-linked
            heterodimer composed of the 40 kD cytokine receptor like subunit
            encoded by this gene, and a 35 kD subunit encoded by IL12A. This
            cytokine is expressed by activated macrophages that serve as an
            essential inducer of Th1 cells development. This cytokine has been
            found to be important for sustaining a sufficient number of
            memory/effector Th1 cells to mediate long-term protection to an
            intracellular pathogen. Overexpression of this gene was observed in
            the central nervous system of patients with multiple sclerosis
            (MS), suggesting a role of this cytokine in the pathogenesis of the
            disease. The promoter polymorphism of this gene has been reported
            to be associated with the severity of atopic and non-atopic asthma
            in children. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M65290.1, M65272.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2152719 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000231228.3/ ENSP00000231228.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AC011418.5         13132-13145
            15-1413             M65272.1           1-1399
            1414-2137           M65290.1           1371-2094
            2138-2364           AC011418.5         28613-28839
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q33.3"
     gene            1..2364
                     /gene="IL12B"
                     /gene_synonym="CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF;
                     NKSF2"
                     /note="interleukin 12B"
                     /db_xref="GeneID:3593"
                     /db_xref="HGNC:HGNC:5970"
                     /db_xref="MIM:161561"
     exon            1..56
                     /gene="IL12B"
                     /gene_synonym="CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF;
                     NKSF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             57..1043
                     /gene="IL12B"
                     /gene_synonym="CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF;
                     NKSF2"
                     /note="interleukin 12, p40; natural killer cell
                     stimulatory factor, 40 kD subunit; interleukin-12 beta
                     chain; IL12, subunit p40; interleukin 12B (natural killer
                     cell stimulatory factor 2, cytotoxic lymphocyte maturation
                     factor 2, p40); interleukin-12 subunit beta; CLMF p40;
                     IL-12 subunit p40; NK cell stimulatory factor chain 2;
                     cytotoxic lymphocyte maturation factor 40 kDa subunit"
                     /codon_start=1
                     /product="interleukin-12 subunit beta precursor"
                     /protein_id="NP_002178.2"
                     /db_xref="CCDS:CCDS4346.1"
                     /db_xref="GeneID:3593"
                     /db_xref="HGNC:HGNC:5970"
                     /db_xref="MIM:161561"
                     /translation="MCHQQLVISWFSLVFLASPLVAIWELKKDVYVVELDWYPDAPGE
                     MVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLL
                     LLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSR
                     GSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKL
                     KYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQ
                     VQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS"
     exon            57..144
                     /gene="IL12B"
                     /gene_synonym="CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF;
                     NKSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            145..420
                     /gene="IL12B"
                     /gene_synonym="CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF;
                     NKSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            421..538
                     /gene="IL12B"
                     /gene_synonym="CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF;
                     NKSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            539..753
                     /gene="IL12B"
                     /gene_synonym="CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF;
                     NKSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            754..911
                     /gene="IL12B"
                     /gene_synonym="CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF;
                     NKSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1043
                     /gene="IL12B"
                     /gene_synonym="CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF;
                     NKSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..2364
                     /gene="IL12B"
                     /gene_synonym="CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF;
                     NKSF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2339..2344
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IL12B"
                     /gene_synonym="CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF;
                     NKSF2"
                     /note="hexamer: AATAAA"
     polyA_site      2364
                     /gene="IL12B"
                     /gene_synonym="CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF;
                     NKSF2"
                     /note="major polyA site"
ORIGIN      
        1 agaagaaaca acatctgttt cagggccatt ggactctccg tcctgcccag agcaagatgt
       61 gtcaccagca gttggtcatc tcttggtttt ccctggtttt tctggcatct cccctcgtgg
      121 ccatatggga actgaagaaa gatgtttatg tcgtagaatt ggattggtat ccggatgccc
      181 ctggagaaat ggtggtcctc acctgtgaca cccctgaaga agatggtatc acctggacct
      241 tggaccagag cagtgaggtc ttaggctctg gcaaaaccct gaccatccaa gtcaaagagt
      301 ttggagatgc tggccagtac acctgtcaca aaggaggcga ggttctaagc cattcgctcc
      361 tgctgcttca caaaaaggaa gatggaattt ggtccactga tattttaaag gaccagaaag
      421 aacccaaaaa taagaccttt ctaagatgcg aggccaagaa ttattctgga cgtttcacct
      481 gctggtggct gacgacaatc agtactgatt tgacattcag tgtcaaaagc agcagaggct
      541 cttctgaccc ccaaggggtg acgtgcggag ctgctacact ctctgcagag agagtcagag
      601 gggacaacaa ggagtatgag tactcagtgg agtgccagga ggacagtgcc tgcccagctg
      661 ctgaggagag tctgcccatt gaggtcatgg tggatgccgt tcacaagctc aagtatgaaa
      721 actacaccag cagcttcttc atcagggaca tcatcaaacc tgacccaccc aagaacttgc
      781 agctgaagcc attaaagaat tctcggcagg tggaggtcag ctgggagtac cctgacacct
      841 ggagtactcc acattcctac ttctccctga cattctgcgt tcaggtccag ggcaagagca
      901 agagagaaaa gaaagataga gtcttcacgg acaagacctc agccacggtc atctgccgca
      961 aaaatgccag cattagcgtg cgggcccagg accgctacta tagctcatct tggagcgaat
     1021 gggcatctgt gccctgcagt taggttctga tccaggatga aaatttggag gaaaagtgga
     1081 agatattaag caaaatgttt aaagacacaa cggaatagac ccaaaaagat aatttctatc
     1141 tgatttgctt taaaacgttt ttttaggatc acaatgatat ctttgctgta tttgtatagt
     1201 tagatgctaa atgctcattg aaacaatcag ctaatttatg tatagatttt ccagctctca
     1261 agttgccatg ggccttcatg ctatttaaat atttaagtaa tttatgtatt tattagtata
     1321 ttactgttat ttaacgtttg tctgccagga tgtatggaat gtttcatact cttatgacct
     1381 gatccatcag gatcagtccc tattatgcaa aatgtgaatt taattttatt tgtactgaca
     1441 acttttcaag caaggctgca agtacatcag ttttatgaca atcaggaaga atgcagtgtt
     1501 ctgataccag tgccatcata cacttgtgat ggatgggaac gcaagagata cttacatgga
     1561 aacctgacaa tgcaaacctg ttgagaagat ccaggagaac aagatgctag ttcccatgtc
     1621 tgtgaagact tcctggagat ggtgttgata aagcaattta gggccactta cacttctaag
     1681 caagtttaat ctttggatgc ctgaatttta aaagggctag aaaaaaatga ttgaccagcc
     1741 tgggaaacat aacaagaccc cgtctctaca aaaaaaattt aaaattagcc aggcgtggtg
     1801 gctcatgctt gtggtcccag ctgttcagga ggatgaggca ggaggatctc ttgagcccag
     1861 gaggtcaagg ctatggtgag ccgtgattgt gccactgcat accagcctag gtgacagaat
     1921 gagaccctgt ctcaaaaaaa aaaatgattg aaattaaaat tcagctttag cttccatggc
     1981 agtcctcacc cccacctctc taaaagacac aggaggatga cacagaaaca ccgtaagtgt
     2041 ctggaaggca aaaagatctt aagattcaag agagaggaca agtagttatg gctaaggaca
     2101 tgaaattgtc agaatggcag gtggcttctt aacagccctg tgagaagcag acagatgcaa
     2161 agaaaatctg gaatcccttt ctcattagca tgaatgaacc tgatacacaa ttatgaccag
     2221 aaaatatggc tccatgaagg tgctactttt aagtaatgta tgtgcgctct gtaaagtgat
     2281 tacatttgtt tcctgtttgt ttatttattt atttattttt gcattctgag gctgaactaa
     2341 taaaaactct tctttgtaat cata
//
