LOCUS       NM_001397992            1444 bp    mRNA    linear   PRI 12-AUG-2024
DEFINITION  Homo sapiens interleukin 12A (IL12A), transcript variant 1, mRNA.
ACCESSION   NM_001397992
VERSION     NM_001397992.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Gao,L., Xiong,Y.J., Liang,Y.X., Huang,P.F., Liu,S., Xiao,Y.,
            Huang,Q., Wang,H. and Wu,H.M.
  TITLE     The effects of IL-27 and IL-35 gene variation and expression levels
            on the susceptibility and clinical manifestations of pulmonary
            tuberculosis
  JOURNAL   Front Immunol 15, 1267624 (2024)
   PUBMED   38690286
  REMARK    GeneRIF: The effects of IL-27 and IL-35 gene variation and
            expression levels on the susceptibility and clinical manifestations
            of pulmonary tuberculosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Sun,H., Ge,Y., Liu,J., Li,Z., Li,H., Zhao,T., Wang,X., Feng,Y.,
            Wang,H., Gao,S., Shi,L., Yang,S., Sun,P., Chang,A., Hao,J. and
            Huang,C.
  TITLE     Tumor-derived interleukin 35 mediates the dissemination of
            gemcitabine resistance in pancreatic adenocarcinoma
  JOURNAL   Oncogene 43 (11), 776-788 (2024)
   PUBMED   38243080
  REMARK    GeneRIF: Tumor-derived interleukin 35 mediates the dissemination of
            gemcitabine resistance in pancreatic adenocarcinoma.
REFERENCE   3  (bases 1 to 1444)
  AUTHORS   Hourani,T., Eivazitork,M., Balendran,T., Mc Lee,K., Hamilton,J.A.,
            Zhu,H.J., Iaria,J., Morokoff,A.P., Luwor,R.B. and Achuthan,A.A.
  TITLE     Signaling pathways underlying TGF-beta mediated suppression of
            IL-12A gene expression in monocytes
  JOURNAL   Mol Immunol 166, 101-109 (2024)
   PUBMED   38278031
  REMARK    GeneRIF: Signaling pathways underlying TGF-beta mediated
            suppression of IL-12A gene expression in monocytes.
REFERENCE   4  (bases 1 to 1444)
  AUTHORS   Zysk,W., Sitko,K., Tukaj,S., Zaryczanska,A. and Trzeciak,M.
  TITLE     Altered Gene Expression of IL-35 and IL-36alpha in the Skin of
            Patients with Atopic Dermatitis
  JOURNAL   Int J Mol Sci 25 (1), 404 (2023)
   PUBMED   38203575
  REMARK    GeneRIF: Altered Gene Expression of IL-35 and IL-36alpha in the
            Skin of Patients with Atopic Dermatitis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1444)
  AUTHORS   Hildenbrand,K., Bohnacker,S., Menon,P.R., Kerle,A.,
            Prodjinotho,U.F., Hartung,F., Strasser,P.C., Catici,D.A.M.,
            Ruhrnossl,F., Haslbeck,M., Schumann,K., Muller,S.I., da Costa,C.P.,
            Esser-von Bieren,J. and Feige,M.J.
  TITLE     Human interleukin-12alpha and EBI3 are cytokines with
            anti-inflammatory functions
  JOURNAL   Sci Adv 9 (43), eadg6874 (2023)
   PUBMED   37878703
  REMARK    GeneRIF: Human interleukin-12alpha and EBI3 are cytokines with
            anti-inflammatory functions.
REFERENCE   6  (bases 1 to 1444)
  AUTHORS   D'Andrea,A., Rengaraju,M., Valiante,N.M., Chehimi,J., Kubin,M.,
            Aste,M., Chan,S.H., Kobayashi,M., Young,D., Nickbarg,E. et al.
  TITLE     Production of natural killer cell stimulatory factor (interleukin
            12) by peripheral blood mononuclear cells
  JOURNAL   J Exp Med 176 (5), 1387-1398 (1992)
   PUBMED   1357073
REFERENCE   7  (bases 1 to 1444)
  AUTHORS   Sieburth,D., Jabs,E.W., Warrington,J.A., Li,X., Lasota,J.,
            LaForgia,S., Kelleher,K., Huebner,K., Wasmuth,J.J. and Wolf,S.F.
  TITLE     Assignment of genes encoding a unique cytokine (IL12) composed of
            two unrelated subunits to chromosomes 3 and 5
  JOURNAL   Genomics 14 (1), 59-62 (1992)
   PUBMED   1358798
REFERENCE   8  (bases 1 to 1444)
  AUTHORS   Schoenhaut,D.S., Chua,A.O., Wolitzky,A.G., Quinn,P.M., Dwyer,C.M.,
            McComas,W., Familletti,P.C., Gately,M.K. and Gubler,U.
  TITLE     Cloning and expression of murine IL-12
  JOURNAL   J Immunol 148 (11), 3433-3440 (1992)
   PUBMED   1350290
REFERENCE   9  (bases 1 to 1444)
  AUTHORS   Wolf,S.F., Temple,P.A., Kobayashi,M., Young,D., Dicig,M., Lowe,L.,
            Dzialo,R., Fitz,L., Ferenz,C., Hewick,R.M. et al.
  TITLE     Cloning of cDNA for natural killer cell stimulatory factor, a
            heterodimeric cytokine with multiple biologic effects on T and
            natural killer cells
  JOURNAL   J Immunol 146 (9), 3074-3081 (1991)
   PUBMED   1673147
REFERENCE   10 (bases 1 to 1444)
  AUTHORS   Stern,A.S., Podlaski,F.J., Hulmes,J.D., Pan,Y.C., Quinn,P.M.,
            Wolitzky,A.G., Familletti,P.C., Stremlo,D.L., Truitt,T.,
            Chizzonite,R. et al.
  TITLE     Purification to homogeneity and partial characterization of
            cytotoxic lymphocyte maturation factor from human B-lymphoblastoid
            cells
  JOURNAL   Proc Natl Acad Sci U S A 87 (17), 6808-6812 (1990)
   PUBMED   2204066
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010370.5 and M65291.1.
            
            Summary: This gene encodes a subunit of a cytokine that acts on T
            and natural killer cells, and has a broad array of biological
            activities. The cytokine is a disulfide-linked heterodimer composed
            of the 35-kD subunit encoded by this gene, and a 40-kD subunit that
            is a member of the cytokine receptor family. This cytokine is
            required for the T-cell-independent induction of interferon
            (IFN)-gamma, and is important for the differentiation of both Th1
            and Th2 cells. The responses of lymphocytes to this cytokine are
            mediated by the activator of transcription protein STAT4. Nitric
            oxide synthase 2A (NOS2A/NOS2) is found to be required for the
            signaling process of this cytokine in innate immunity. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. The encoded isoform (4) uses a downstream in-frame
            start codon and is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M65291.1, BC104984.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000699704.1/
                                                ENSP00000514529.1
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-122               AC010370.5         107708-107829
            123-353             M65291.1           2-232
            354-360             AC010370.5         114886-114892
            361-1443            M65291.1           234-1316
            1444-1450           AC010370.5         114886-114892
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q25.33"
     gene            1..1444
                     /gene="IL12A"
                     /gene_synonym="CLMF; IL-12A; NFSK; NKSF1; P35"
                     /note="interleukin 12A"
                     /db_xref="GeneID:3592"
                     /db_xref="HGNC:HGNC:5969"
                     /db_xref="MIM:161560"
     exon            1..340
                     /gene="IL12A"
                     /gene_synonym="CLMF; IL-12A; NFSK; NKSF1; P35"
                     /inference="alignment:Splign:2.1.0"
     CDS             325..984
                     /gene="IL12A"
                     /gene_synonym="CLMF; IL-12A; NFSK; NKSF1; P35"
                     /note="isoform 4 precursor is encoded by transcript
                     variant 1; IL35 subunit; cytotoxic lymphocyte maturation
                     factor 1, p35; interleukin 12, p35; IL-12, subunit p35; NF
                     cell stimulatory factor chain 1; interleukin-12 alpha
                     chain; interleukin-12 subunit alpha; CLMF p35; NK cell
                     stimulatory factor chain 1; cytotoxic lymphocyte
                     maturation factor 35 kDa subunit; interleukin 12A (natural
                     killer cell stimulatory factor 1, cytotoxic lymphocyte
                     maturation factor 1, p35)"
                     /codon_start=1
                     /product="interleukin-12 subunit alpha isoform 4
                     precursor"
                     /protein_id="NP_001384921.1"
                     /db_xref="CCDS:CCDS93420.1"
                     /db_xref="GeneID:3592"
                     /db_xref="HGNC:HGNC:5969"
                     /db_xref="MIM:161560"
                     /translation="MCPARSLLLVATLVLLDHLSLARNLPVATPDPGMFPCLHHSQNL
                     LRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRET
                     SFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNML
                     AVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNA
                     S"
     exon            341..486
                     /gene="IL12A"
                     /gene_synonym="CLMF; IL-12A; NFSK; NKSF1; P35"
                     /inference="alignment:Splign:2.1.0"
     exon            487..600
                     /gene="IL12A"
                     /gene_synonym="CLMF; IL-12A; NFSK; NKSF1; P35"
                     /inference="alignment:Splign:2.1.0"
     exon            601..642
                     /gene="IL12A"
                     /gene_synonym="CLMF; IL-12A; NFSK; NKSF1; P35"
                     /inference="alignment:Splign:2.1.0"
     exon            643..684
                     /gene="IL12A"
                     /gene_synonym="CLMF; IL-12A; NFSK; NKSF1; P35"
                     /inference="alignment:Splign:2.1.0"
     exon            685..828
                     /gene="IL12A"
                     /gene_synonym="CLMF; IL-12A; NFSK; NKSF1; P35"
                     /inference="alignment:Splign:2.1.0"
     exon            829..1444
                     /gene="IL12A"
                     /gene_synonym="CLMF; IL-12A; NFSK; NKSF1; P35"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1421..1426
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IL12A"
                     /gene_synonym="CLMF; IL-12A; NFSK; NKSF1; P35"
                     /note="hexamer: AATAAA"
     polyA_site      1444
                     /gene="IL12A"
                     /gene_synonym="CLMF; IL-12A; NFSK; NKSF1; P35"
                     /note="major polyA site"
ORIGIN      
        1 atttcgcttt cattttgggc cgagctggag gcggcggggc cgtcccggaa cggctgcggc
       61 cgggcacccc gggagttaat ccgaaagcgc cgcaagcccc gcgggccggc cgcaccgcac
      121 gtgtcaccga gaagctgatg tagagagaga cacagaagga gacagaaagc aagagaccag
      181 agtcccggga aagtcctgcc gcgcctcggg acaattataa aaatgtggcc ccctgggtca
      241 gcctcccagc caccgccctc acctgccgcg gccacaggtc tgcatccagc ggctcgccct
      301 gtgtccctgc agtgccggct cagcatgtgt ccagcgcgca gcctcctcct tgtggctacc
      361 ctggtcctcc tggaccacct cagtttggcc agaaacctcc ccgtggccac tccagaccca
      421 ggaatgttcc catgccttca ccactcccaa aacctgctga gggccgtcag caacatgctc
      481 cagaaggcca gacaaactct agaattttac ccttgcactt ctgaagagat tgatcatgaa
      541 gatatcacaa aagataaaac cagcacagtg gaggcctgtt taccattgga attaaccaag
      601 aatgagagtt gcctaaattc cagagagacc tctttcataa ctaatgggag ttgcctggcc
      661 tccagaaaga cctcttttat gatggccctg tgccttagta gtatttatga agacttgaag
      721 atgtaccagg tggagttcaa gaccatgaat gcaaagcttc tgatggatcc taagaggcag
      781 atctttctag atcaaaacat gctggcagtt attgatgagc tgatgcaggc cctgaatttc
      841 aacagtgaga ctgtgccaca aaaatcctcc cttgaagaac cggattttta taaaactaaa
      901 atcaagctct gcatacttct tcatgctttc agaattcggg cagtgactat tgatagagtg
      961 atgagctatc tgaatgcttc ctaaaaagcg aggtccctcc aaaccgttgt catttttata
     1021 aaactttgaa atgaggaaac tttgatagga tgtggattaa gaactaggga gggggaaaga
     1081 aggatgggac tattacatcc acatgatacc tctgatcaag tatttttgac atttactgtg
     1141 gataaattgt ttttaagttt tcatgaatga attgctaaga agggaaaata tccatcctga
     1201 aggtgttttt cattcacttt aatagaaggg caaatattta taagctattt ctgtaccaaa
     1261 gtgtttgtgg aaacaaacat gtaagcataa cttattttaa aatatttatt tatataactt
     1321 ggtaatcatg aaagcatctg agctaactta tatttattta tgttatattt attaaattat
     1381 ttatcaagtg tatttgaaaa atatttttaa gtgttctaaa aataaaagta ttgaattaaa
     1441 gtga
//
