LOCUS       NM_001387220            9562 bp    mRNA    linear   PRI 07-DEC-2024
DEFINITION  Homo sapiens IKAROS family zinc finger 2 (IKZF2), transcript
            variant 7, mRNA.
ACCESSION   NM_001387220
VERSION     NM_001387220.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Lukasik,M., Telec,M., Kazmierski,R., Wojtasz,I.,
            Andrzejewska-Gorczynska,N., Kociemba,W., Dworacki,G., Kozubski,W.P.
            and Frydrychowicz,M.
  TITLE     Temporal changes in regulatory T cell subsets defined by the
            transcription factor Helios in stroke and their potential role in
            stroke-associated infection: a prospective case-control study
  JOURNAL   J Neuroinflammation 20 (1), 275 (2023)
   PUBMED   37996909
  REMARK    GeneRIF: Temporal changes in regulatory T cell subsets defined by
            the transcription factor Helios in stroke and their potential role
            in stroke-associated infection: a prospective case-control study.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   Mohajeri,A., Vaseghi-Shanjani,M., Rosenfeld,J.A., Yang,G.X., Lu,H.,
            Sharma,M., Lin,S., Salman,A., Waqas,M., Sababi Azamian,M.,
            Worley,K.C., Del Bel,K.L., Kozak,F.K., Rahmanian,R., Biggs,C.M.,
            Hildebrand,K.J., Lalani,S.R., Nicholas,S.K., Scott,D.A.,
            Mostafavi,S., van Karnebeek,C., Henkelman,E., Halparin,J.,
            Yang,C.L., Armstrong,L., Turvey,S.E. and Lehman,A.
  CONSRTM   Undiagnosed Diseases Network; Care4Rare Canada Consortium
  TITLE     Dominant negative variants in IKZF2 cause ICHAD syndrome, a new
            disorder characterised by immunodysregulation, craniofacial
            anomalies, hearing impairment, athelia and developmental delay
  JOURNAL   J Med Genet 60 (11), 1092-1104 (2023)
   PUBMED   37316189
  REMARK    GeneRIF: Dominant negative variants in IKZF2 cause ICHAD syndrome,
            a new disorder characterised by immunodysregulation, craniofacial
            anomalies, hearing impairment, athelia and developmental delay.
REFERENCE   3  (bases 1 to 9562)
  AUTHORS   Tarighi,M., Shahbazi,M., Saadat,P., Daraei,A., Alizadeh Khatir,A.,
            Rahimifard,K. and Mohammadnia-Afrouzi,M.
  TITLE     Decreased frequency of regulatory T cells and level of helios gene
            expression in secondary progressive multiple sclerosis patients:
            Evidence about the development of multiple sclerosis
  JOURNAL   Int Immunopharmacol 116, 109797 (2023)
   PUBMED   36738680
  REMARK    GeneRIF: Decreased frequency of regulatory T cells and level of
            helios gene expression in secondary progressive multiple sclerosis
            patients: Evidence about the development of multiple sclerosis.
REFERENCE   4  (bases 1 to 9562)
  AUTHORS   Zhang,H., Jadhav,R.R., Cao,W., Goronzy,I.N., Zhao,T.V., Jin,J.,
            Ohtsuki,S., Hu,Z., Morales,J., Greenleaf,W.J., Weyand,C.M. and
            Goronzy,J.J.
  TITLE     Aging-associated HELIOS deficiency in naive CD4+ T cells alters
            chromatin remodeling and promotes effector cell responses
  JOURNAL   Nat Immunol 24 (1), 96-109 (2023)
   PUBMED   36510022
  REMARK    GeneRIF: Aging-associated HELIOS deficiency in naive CD4[+] T cells
            alters chromatin remodeling and promotes effector cell responses.
REFERENCE   5  (bases 1 to 9562)
  AUTHORS   Pieren,D.K.J., Smits,N.A.M., Postel,R.J., Kandiah,V., de Wit,J.,
            van Beek,J., van Baarle,D. and Guichelaar,T.
  TITLE     Co-Expression of TIGIT and Helios Marks Immunosenescent CD8+ T
            Cells During Aging
  JOURNAL   Front Immunol 13, 833531 (2022)
   PUBMED   35651622
  REMARK    GeneRIF: Co-Expression of TIGIT and Helios Marks Immunosenescent
            CD8(+) T Cells During Aging.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9562)
  AUTHORS   Nakase,K., Ishimaru,F., Fujii,K., Tabayashi,T., Kozuka,T.,
            Sezaki,N., Matsuo,Y. and Harada,M.
  TITLE     Overexpression of novel short isoforms of Helios in a patient with
            T-cell acute lymphoblastic leukemia
  JOURNAL   Exp Hematol 30 (4), 313-317 (2002)
   PUBMED   11937265
  REMARK    GeneRIF: Novel short isoforms of Helios are overexpressed in a
            patient with T-cell acute lymphoblastic leukemia and may contribute
            to the development of T-cell malignancies. Data for unaltered
            Helios indicates tumor suppressor function.
REFERENCE   7  (bases 1 to 9562)
  AUTHORS   Perdomo,J., Holmes,M., Chong,B. and Crossley,M.
  TITLE     Eos and pegasus, two members of the Ikaros family of proteins with
            distinct DNA binding activities
  JOURNAL   J Biol Chem 275 (49), 38347-38354 (2000)
   PUBMED   10978333
REFERENCE   8  (bases 1 to 9562)
  AUTHORS   Hosokawa,Y., Maeda,Y. and Seto,M.
  TITLE     Human Helios, an Ikaros-related zinc finger DNA binding protein:
            cDNA cloning and tissue expression pattern
  JOURNAL   Immunogenetics 50 (1-2), 106-108 (1999)
   PUBMED   10541817
REFERENCE   9  (bases 1 to 9562)
  AUTHORS   Kelley,C.M., Ikeda,T., Koipally,J., Avitahl,N., Wu,L.,
            Georgopoulos,K. and Morgan,B.A.
  TITLE     Helios, a novel dimerization partner of Ikaros expressed in the
            earliest hematopoietic progenitors
  JOURNAL   Curr Biol 8 (9), 508-515 (1998)
   PUBMED   9560339
REFERENCE   10 (bases 1 to 9562)
  AUTHORS   Hahm,K., Cobb,B.S., McCarty,A.S., Brown,K.E., Klug,C.A., Lee,R.,
            Akashi,K., Weissman,I.L., Fisher,A.G. and Smale,S.T.
  TITLE     Helios, a T cell-restricted Ikaros family member that
            quantitatively associates with Ikaros at centromeric
            heterochromatin
  JOURNAL   Genes Dev 12 (6), 782-796 (1998)
   PUBMED   9512513
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC079610.4, AC013736.9 and
            AC108066.3.
            
            Summary: This gene encodes a member of the Ikaros family of
            zinc-finger proteins. Three members of this protein family (Ikaros,
            Aiolos and Helios) are hematopoietic-specific transcription factors
            involved in the regulation of lymphocyte development. This protein
            forms homo- or hetero-dimers with other Ikaros family members, and
            is thought to function predominantly in early hematopoietic
            development. Multiple transcript variants encoding different
            isoforms have been found for this gene, but the biological validity
            of some variants has not been determined. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.3599081.1,
                                           SRR14038192.2525778.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000434687.6/ ENSP00000412869.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-200               AC079610.4         11230-11429         c
            201-304             AC079610.4         9961-10064          c
            305-353             AC079610.4         8413-8461           c
            354-458             AC079610.4         7525-7629           c
            459-725             AC013736.9         8228-8494           c
            726-893             AC013736.9         1108-1275           c
            894-1031            AC108066.3         129987-130124       c
            1032-1175           AC108066.3         121785-121928       c
            1176-9562           AC108066.3         107692-116078       c
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q34"
     gene            1..9562
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /note="IKAROS family zinc finger 2"
                     /db_xref="GeneID:22807"
                     /db_xref="HGNC:HGNC:13177"
                     /db_xref="MIM:606234"
     exon            1..200
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            201..304
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            305..353
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /inference="alignment:Splign:2.1.0"
     CDS             320..1900
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /note="isoform 1 is encoded by transcript variant 7; zinc
                     finger protein, subfamily 1A, 2 (Helios); zinc finger DNA
                     binding protein Helios; zinc finger protein Helios; ikaros
                     family zinc finger protein 2"
                     /codon_start=1
                     /product="zinc finger protein Helios isoform 1"
                     /protein_id="NP_001374149.1"
                     /db_xref="CCDS:CCDS2395.1"
                     /db_xref="GeneID:22807"
                     /db_xref="HGNC:HGNC:13177"
                     /db_xref="MIM:606234"
                     /translation="METEAIDGYITCDNELSPEREHSNMAIDLTSSTPNGQHASPSHM
                     TSTNSVKLEMQSDEECDRKPLSREDEIRGHDEGSSLEEPLIESSEVADNRKVQELQGE
                     GGIRLPNGKLKCDVCGMVCIGPNVLMVHKRSHTGERPFHCNQCGASFTQKGNLLRHIK
                     LHSGEKPFKCPFCSYACRRRDALTGHLRTHSVGKPHKCNYCGRSYKQRSSLEEHKERC
                     HNYLQNVSMEAAGQVMSHHVPPMEDCKEQEPIMDNNISLVPFERPAVIEKLTGNMGKR
                     KSSTPQKFVGEKLMRFSYPDIHFDMNLTYEKEAELMQSHMMDQAINNAITYLGAEALH
                     PLMQHPPSTIAEVAPVISSAYSQVYHPNRIERPISRETADSHENNMDGPISLIRPKSR
                     PQEREASPSNSCLDSTDSESSHDDHQSYQGHPALNPKRKQSPAYMKEDVKALDTTKAP
                     KGSLKDIYKVFNGEGEQIRAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRS
                     QDRYEFSSHIVRGEHTFH"
     exon            354..458
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            459..725
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            726..893
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1031
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1175
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..9562
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9542..9547
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /note="hexamer: AATAAA"
     polyA_site      9562
                     /gene="IKZF2"
                     /gene_synonym="ANF1A2; HELIOS; ZNF1A2; ZNFN1A2"
                     /note="major polyA site"
ORIGIN      
        1 gctaaccctg ctcctcgctg aagatggagg aagtaaaaac aggattaccc ttagctacag
       61 atccactgcc ttagtttcca ccaccaactg cagtgcacaa acacacgtta ggcacaggaa
      121 agaaagaaag acagaggaca cattaacagt aaacacaaac aaaagggtga tgggattatt
      181 ttactgcatg cactgctgag cccgacattg tcacctcctc tttgaggggt tagaagaagc
      241 tgagatctcc cgacagagct ggaaatggtg atgaatcttt tttaatcaaa ggacaatttc
      301 ttttcattgc actttgacta tggaaacaga ggctattgat ggctatataa cgtgtgacaa
      361 tgagctttca cccgaaaggg agcactccaa tatggcaatt gacctcacct caagcacacc
      421 caatggacag catgcctcac caagtcacat gacaagcaca aattcagtaa agctagaaat
      481 gcagagtgat gaagagtgtg acaggaaacc cctgagccgt gaagatgaga tcaggggcca
      541 tgatgagggt agcagcctag aagaacccct aattgagagc agcgaggtgg ctgacaacag
      601 gaaagtccag gagcttcaag gcgagggagg aatccggctt ccgaatggta aactgaaatg
      661 tgacgtctgt ggcatggttt gcattgggcc caatgtgctt atggtacata aaaggagtca
      721 cactggtgaa cgccccttcc actgtaacca gtgtggagct tcttttactc agaagggcaa
      781 ccttctgaga cacataaagt tacactctgg agagaagccg ttcaaatgtc ctttctgtag
      841 ctacgcctgt agaagaaggg acgccctcac aggacacctc aggacccatt ctgtgggtaa
      901 acctcacaag tgcaactact gtggacgaag ctacaagcag cgcagttcac tggaggagca
      961 caaggaacgc tgccacaact atctccagaa tgtcagcatg gaggctgctg ggcaggtcat
     1021 gagtcaccat gtacctccta tggaagattg taaggaacaa gagcctatta tggacaacaa
     1081 tatttctctg gtgccttttg agagacctgc tgtcatagag aagctcacgg ggaatatggg
     1141 aaaacgtaaa agctccactc cacaaaagtt tgtgggggaa aagctcatgc gattcagcta
     1201 cccagatatt cactttgata tgaacttaac atatgagaag gaggctgagc tgatgcagtc
     1261 tcatatgatg gaccaagcca tcaacaatgc aatcacctac cttggagctg aggcccttca
     1321 ccctctgatg cagcacccgc caagcacaat cgctgaagtg gccccagtta taagctcagc
     1381 ttattctcag gtctatcatc caaataggat agaaagaccc attagcaggg aaactgctga
     1441 tagtcatgaa aacaacatgg atggccccat ctctctcatc agaccaaaga gtcgacccca
     1501 ggaaagagag gcctctccca gcaatagctg cctggattcc actgactcag aaagcagcca
     1561 tgatgaccac cagtcctacc aaggacaccc tgccttaaat cccaagagga aacaaagccc
     1621 agcttacatg aaggaggatg tcaaagcttt ggatactacc aaggctccta agggctctct
     1681 gaaggacatc tacaaggtct tcaatggaga aggagaacag attagggcct tcaagtgtga
     1741 gcactgccga gtccttttcc tagaccatgt catgtacacc attcacatgg gttgccatgg
     1801 ctaccgggac ccactggaat gcaacatctg tggctacaga agccaggacc gttatgagtt
     1861 ttcatcacac attgttcgag gggagcacac attccactag gccttttcat tccaaagggg
     1921 acccctatga agtaaagaac tgcacatgaa gaaatactgc acttacaatc ccacctttcc
     1981 tcaaatgttg acataccttt tatttttttt aatattatta ctgttgataa ttcttatttt
     2041 gtggaggcag tgtcatttgc tctgcctaat tacgataagg aagaaacaga agagagaagg
     2101 ggcgggaata ttgtttcttt atcacctggc ttgtttattt tgtgggaatt taagagcagt
     2161 ccatttctac caaggcatat catgctttga aaaatcactt gattcataaa gattcaccta
     2221 agagattctg atttgccact gatattcaga attatgatgg aagacaggaa agttcagagt
     2281 tttctgggta ggactttggt ggtttaaaaa tggtataagt aactttattc ttgaaagaag
     2341 aatgtgtttc aaactgtaaa ccaatttttt gttcttcaga gatcatggaa cacaaacaca
     2401 ttgttatttt cagtgataac tcctaagagg agctgagttg ttgtgggttc tatgtttact
     2461 tcccctatgg aatttataat tcagtatgtt ttacactgta ccatatagca aaacttttaa
     2521 actacaggta gttaagggcc acctacaata catctgaggt cctgtgatct tatttttcta
     2581 aacgtaagca ctgtttttcc atagttttga tgactggcat tttatagaca ccctggcagc
     2641 cttactttta acacctttaa ggaatagtat ttttatgtag ttttcagaat aacatatggt
     2701 ctaagagtgg ataaaaggca gtcaataatt tctgggaggg acttctactt tcataaattt
     2761 gtttgagagg ttttctttta aagttgtaat gtgatggcag catagtatat gtatttgttt
     2821 ctaaaagtat gcttacgatt gtcactttat cagcatttaa tcagtgttaa ccagtcagca
     2881 gaaaaatata attatgctaa cagtaggggg agaaaaccca cttagaaatc ccttttctgg
     2941 tatttctctt ttcactagtt tttttcaaga tgtgacctcc cggtgttctg tccatagttc
     3001 attcatcctt tactcttcga gtagaaggtc ttaaaagtct tcctgtcggc tgtttctttc
     3061 aaaatctcct cagagcaatt gctaatttgg cctgaatctg gtaacttgaa ccctgtaagg
     3121 ttacagaact agggctattt attttagcat ttcttcagta gtatttacta ctcttgttgc
     3181 aaagaaaagg gaatgggact tctttgtaac ctgtaccttg gacaacagat aaaagaaaca
     3241 aaaaaataag aaagtttact tttacccttc ttggagtcta gaatgtgaca gaacccccaa
     3301 aggaaagtcc tgcacatttt tctgtttcca aaacatttaa ttgtgtaagt ccttgtcaga
     3361 aatgaatctc aatcccttag tatagaattc cccttacatg gtataggttg ccatatttca
     3421 tgtgcagatt ttaatttcat ttatgtgggc gctctgtttt ttctttgcag tccagccaca
     3481 ttagagggga ggaaccgagt gatattgatt caagtcattt tagggggaca tacttggaag
     3541 gcagaacttg ctgcttctgt ttggggagga cagacctgac tgtgactgga ttatctgata
     3601 accatttgtg aatactgaaa ttctgttagg cagtaactga taactgctct aaaggatcat
     3661 taaataggat gctgaaatta tgtatcttaa tacagtgtgg tatgagaatt accaagtcaa
     3721 gagaattgtg gacataagca agtttggccc caatactgct cttaactcat tttccagctt
     3781 actatttgct atttaaatgg taggcaccag ctaagcactt ctaagcacta acacagctag
     3841 aactaggcaa aaatggttag aactcagctc tcttctacta gtccctgtca taattatttt
     3901 tgggaaaatg tccaaactgc cccctttaaa tctaagggaa tgcaccaaaa cagagatata
     3961 tagaatgtca accatttcat tttttttttt ctgcatgcct tggtacatag tgaacataca
     4021 acctatttaa agataaagca tgtttttgag actcgctcac ccccccccac ccaaccactc
     4081 ccaaataata attgggatgc catttttttt ccttttggat gaggtaaata attttaaggt
     4141 tcacaatttt gtcttttact gcaatttaag gaaacatttg gatgtcagtc aatatgttca
     4201 taattttggc tgtgtgcgaa tttctgctgg cattatctat gaattttctt cctacttatt
     4261 tttttttcag tatatgaaca atcatgtatc tacctgcccc aggatgaaac taaatttagg
     4321 tggaccctaa accttatgaa gacagtgctg aggcactttc cttttctgat ttcatctttt
     4381 tgggaatctg ttttattgaa ggtagttagt agttgagagt gcatttgcta caagcatata
     4441 cttgtatctt cctagcttca tgaggaacag aaagaggtgg atatggctca gggtgtggca
     4501 gggacaattg aggacaaagt caattcaaat ttgtgggtca gaaagaattt ttgtggacgt
     4561 agtgtttttg gagaaactct ggatggttat atgtgcatgc cttttcttca aaaggaaata
     4621 cgcaaggttg tagcatctaa aaataaacat aagagtcaga caccaaataa atcaagtttt
     4681 acataacagt tgtatgccca gtttgtttag gtgagatttc acattacaga aagtatttga
     4741 ggagcatgaa aatgggttat cttctgtatt ttccagtttg gcaaaagttc agaatttcat
     4801 cacattgctt tgccctaatt ttgcccagaa ttttatctta gcctctctct gacagtgatg
     4861 aatcatgctc aaaagccatt ctaattggac ctttttaaga cagggaaagg gatcagtagg
     4921 cggattggaa gaaatttcaa gtcattgaaa tattccattg agatttccta aagggacaaa
     4981 attgggaaaa taagaaacta cgacttagat ttggctacgt agtagaaagt atctccccta
     5041 catacataca ggcaattgta tgtatgaatc atagggtata tgtgtgtgta tactacacac
     5101 acattctttt aaagagaatt catggaaaaa aaagcagttg gagtgatcag atgtattgca
     5161 aaaacataca gagaatttaa atgacagtta ataccaagaa attagttggg tttactttat
     5221 caggtcgtaa taggaatcac taaagaagtt actagtgtgt ctttaggacc agtggcaact
     5281 cttaaactaa aactttgggt ccttattatc tacttacaga acaaagtgaa acaaacaatg
     5341 attaagctga ttggatatac attcaaagat atttaatgta aagttttttg gaatacgaag
     5401 aaaattcaga aaataaatat tatcaacagt tacttattgg caaatagaga aagacaagaa
     5461 tagtttagtg agcccggtat tttgttttta tagtttttat ctcagttgta caactcacaa
     5521 aaccatgaag tctttggtat tttataaatg tttaacaaaa tttacatcag attaaggcat
     5581 ttagatgaaa attattatgt tctcactatc ttccaaattt tatttcatcc tatctccaaa
     5641 atgatttctt agggtacaaa aagagcagac ggggctgtaa aaatacaagc aaaaaactgt
     5701 gtgcccctag tttcaggcag aacttaaact gtcagaggta ctagctacat gatttgtttt
     5761 ttaactttgg attgttcacg tccaaaaatg gataaattac atttgtgttt atcatcagtt
     5821 gcattttatg tattatttta ataaatacta tctgaatgaa gactattcta aaccagaaaa
     5881 ttccccaaat ccaaaagaaa aaaaaagtgg gaagaggtga aattgaagtt tgtgtatatg
     5941 aaagttatct tagacatatt tttaattctc cagtttctgc aaaataatta aaatatacag
     6001 taactggtct cctaaatcct gaatttaatg tattaaatac ttatgttctt tatattggtg
     6061 cctttttaaa atgcattgag agtgttggtt agctgttgca gctgtacaac acttttaata
     6121 tgcattttta aaaatcactt aaaattgagt actatataat tcatctctgc atttttagtg
     6181 caaatcttta gagcaatttc taatagagaa attttcagct cagctgttaa aaggaaaagg
     6241 aaactttgaa actagacttt actacctttt tagtttcata gtatttctga atatgattac
     6301 aagattatgc aggtaaaata tagagtgaaa ctttacctgt gaattgaatt ataatttgtg
     6361 tttttgtttt gtttttaagg aagaataagt tctgtatcaa acaagaattt attagataat
     6421 tttttggtca ataaaataca gtattcattt ggattttcat ctccagacta gtattgttct
     6481 agtcttggaa tctgtatttt ctaatctgtt agaaaataga gattgaaaat tgatggaata
     6541 atgtgaaaaa gcaggtaatt aattctcctt gaacaaagca aaactgaaca gtcatatcac
     6601 attgctattc tccaaagcat aatctcaaat ggtttcatat catggttgtg tattacttgc
     6661 aatgggtgtg ttaggatatg acagcttttt aaaaaaatga gctgctggtt atacaaagca
     6721 aatggcatat gaccaagaag ctgtgatatg ctagtgtttc tttttatcat agtgtattac
     6781 taggccaaat aatgacacct tgaatatttt tacatttatt gcagaaacct taaactttgg
     6841 aatttccata aggtttttat gtaatattct atttctagct ttttagtttt atcttgctgt
     6901 actgtaagtt tgaggatatt tttcacctgc actcttagga ataagttcat aattctgttt
     6961 atggggcttt cctcccataa cactgcattt gtatattttc tgtataaaat atgtgttgtg
     7021 tattaacctt tatcccatac agagagtggt acatgaatga ctagttttct aagatgtcct
     7081 ttttattgtg aataaaatat aaaagttaaa ggccctctgc taagtcacat aaagtacagc
     7141 atataagttc atataggtac aaataaatga gtttgcagtg aattgggcct tcaaattacc
     7201 tcaagtgaca gatagtaaga aaagcttctt gagcaggtgg aggtcactga atcccctact
     7261 atgcacttac caagatttta cttactttaa tttactggaa attgattttt taaaaaatga
     7321 ctacactgta acaagggaag ggatctgggt ttttttgttg ttttattctt gtttttttta
     7381 agtagttcaa attctgaaac tgtgatttaa aaatttttta cagtcaagca ttctgatttt
     7441 gaacataact cccttccctt tctgtgtaac aaaggtctct ctgttatctc ttaaattttg
     7501 ttacatctcc ctcagcctct ttctttgtcc gtctcccttc tgtcattgtc tatggatgtt
     7561 tacctctctg ttctcctaaa agtttgaaga ttaggtcaac tcttatttct agttcattgg
     7621 taatttaatc ttaatttttt tttcgtgatt tttgttggtt gtataatctg ctgacgtatt
     7681 tttatactca agtgtagttt tctattaaaa agaaaagtgg ttggattaaa aatagtaagc
     7741 tatgtaaccc tcatgttact ttcactttca aatattgggt acctaaaaca ttacttcaga
     7801 gattatgtaa tcctattata gtatgtttgc tttcctttat tgttggattt tacattctga
     7861 tttggctttc ctccaaaaaa tgtatatcat gaaagactag acagttattt gcaagtgttt
     7921 agaaaggtgt taaaaatgta aagcaaagag tcttaacttt ctcctaattg ggagaaaaat
     7981 gctttaacat tactataata atattccagg tttggagggg gtctccaggc cccatatttg
     8041 ctgttaatag ttggaccttt tagaccatgt gttatttgca atcccagaat gattgcttct
     8101 gctattagtt aaaaagatac tattcttttc tttctgtaca agtgcaatac tccccttgaa
     8161 gtcttaaaaa ctatggtgat ttttttttct tttctgacct attcttcctt tagctaatga
     8221 caaaaagaaa ctcataaaag tcatagtatg ttaaaggaca caacaagcaa agagaaaaac
     8281 actccacaat caaaagatta cagaatgtgg aaaccactag tctgatctca tggtatcttt
     8341 atttaagcta aatttccatg gaaattagta atcttttgct tgaaaaatgt gtcctaaagt
     8401 tgaacttttt acagattgaa tcttcttaga ccctcgccca atgctctaaa ttaagaacct
     8461 aatacttaat atttttattt tacttctccc cttttagaaa taaactttta aataaaagca
     8521 aagcacttag ctgagtttta aacacttaca tatcacctat tggagaaatt ttttttaaaa
     8581 atatttggag cagtcctgtt ttcatacaaa tttaagtaag aggtattttt cttatacata
     8641 tttatatgta gtgtgctaat tttctttttt tatacctgtg tccctgtagt aaaactgctg
     8701 taatataaat acatgttttg ttaaaagata acatttcttt ggcatttctt ttaaaggcag
     8761 ttactgcatt tctgcatttg tacagtatgt gtcttggcca ttttagatat tctttcttta
     8821 acaataccaa aggtaattag actattttaa agactaattg cttgacagtt tctagggtat
     8881 tttgtgtttt agaagcaaaa aaagaaaaaa aaataggtca aaccagtaaa cctcattttt
     8941 tttcaaacta ataatttggg gaaataaaaa ctattgttta aaaaagaaat atatatatat
     9001 atatataaat atatatgtaa agttaaaatt ccataccttg tatgtcaggt ttgctaagtg
     9061 taatgtagtt tttttaaggc tcaaatacca tacctcagaa aatgaggttt actatggaaa
     9121 tactgaaaca gtctttgcag ctgtgtgaca agtcactcta ctacatactg atttggagac
     9181 ctccgctaaa tagttttatc actgcagact aaaatgtggg acttgtatct tctttgtttt
     9241 taatgcacac acatacatgt tctgtgcatg tatgtggtta ctgtgtatat gtgtatgagt
     9301 gttgtatatg catgtgtgag tgtgtgtctg tatgtgtgta caactaaaga agctgcagaa
     9361 actttgtaat actttgtgaa aaggattata ttataaaggt ttgtactgtc tgagtgcaca
     9421 gctactggaa taaatttagg gaatctcagg aacaagcata taatttgtcc aagatttatt
     9481 tcttctcaga agtgtaagtg cagtttttaa ttctgtatat tatttaatat tttaccaata
     9541 aaataaactt ctgacataaa aa
//
