LOCUS       NM_006060               6255 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens IKAROS family zinc finger 1 (IKZF1), transcript
            variant 1, mRNA.
ACCESSION   NM_006060 NM_018563
VERSION     NM_006060.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Ichiyama,K., Long,J., Kobayashi,Y., Horita,Y., Kinoshita,T.,
            Nakamura,Y., Kominami,C., Georgopoulos,K. and Sakaguchi,S.
  TITLE     Transcription factor Ikzf1 associates with Foxp3 to repress gene
            expression in Treg cells and limit autoimmunity and anti-tumor
            immunity
  JOURNAL   Immunity 57 (9), 2043-2060 (2024)
   PUBMED   39111316
  REMARK    GeneRIF: Transcription factor Ikzf1 associates with Foxp3 to
            repress gene expression in Treg cells and limit autoimmunity and
            anti-tumor immunity.
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6255)
  AUTHORS   Song,Y., Zhang,Y., Wang,Z., Lin,Y., Cao,X., Han,X., Li,G., Hou,A.
            and Han,S.
  TITLE     CCL2 mediated IKZF1 expression promotes M2 polarization of
            glioma-associated macrophages through CD84-SHP2 pathway
  JOURNAL   Oncogene 43 (36), 2737-2749 (2024)
   PUBMED   39112517
  REMARK    GeneRIF: CCL2 mediated IKZF1 expression promotes M2 polarization of
            glioma-associated macrophages through CD84-SHP2 pathway.
REFERENCE   4  (bases 1 to 6255)
  AUTHORS   Ezzat,S., Yu,S. and Asa,S.L.
  TITLE     Ikaros isoforms in human pituitary tumors: distinct localization,
            histone acetylation, and activation of the 5' fibroblast growth
            factor receptor-4 promoter
  JOURNAL   Am J Pathol 163 (3), 1177-1184 (2003)
   PUBMED   12937159
REFERENCE   5  (bases 1 to 6255)
  AUTHORS   Payne,K.J., Nicolas,J.H., Zhu,J.Y., Barsky,L.W. and Crooks,G.M.
  TITLE     Cutting edge: predominant expression of a novel Ikaros isoform in
            normal human hemopoiesis
  JOURNAL   J Immunol 167 (4), 1867-1870 (2001)
   PUBMED   11489963
  REMARK    GeneRIF: A novel Ikaros isoform 'Ikx' is the predominant Ikaros
            protein present in normal cord blood and bone marrow cells, but not
            in leukemia cell lines.
REFERENCE   6  (bases 1 to 6255)
  AUTHORS   Nakayama,H., Ishimaru,F., Avitahl,N., Sezaki,N., Fujii,N.,
            Nakase,K., Ninomiya,Y., Harashima,A., Minowada,J., Tsuchiyama,J.,
            Imajoh,K., Tsubota,T., Fukuda,S., Sezaki,T., Kojima,K., Hara,M.,
            Takimoto,H., Yorimitsu,S., Takahashi,I., Miyata,A., Taniguchi,S.,
            Tokunaga,Y., Gondo,H., Niho,Y., Harada,M. et al.
  TITLE     Decreases in Ikaros activity correlate with blast crisis in
            patients with chronic myelogenous leukemia
  JOURNAL   Cancer Res 59 (16), 3931-3934 (1999)
   PUBMED   10463586
REFERENCE   7  (bases 1 to 6255)
  AUTHORS   Sun,L., Heerema,N., Crotty,L., Wu,X., Navara,C., Vassilev,A.,
            Sensel,M., Reaman,G.H. and Uckun,F.M.
  TITLE     Expression of dominant-negative and mutant isoforms of the
            antileukemic transcription factor Ikaros in infant acute
            lymphoblastic leukemia
  JOURNAL   Proc Natl Acad Sci U S A 96 (2), 680-685 (1999)
   PUBMED   9892693
REFERENCE   8  (bases 1 to 6255)
  AUTHORS   Molnar,A., Wu,P., Largespada,D.A., Vortkamp,A., Scherer,S.,
            Copeland,N.G., Jenkins,N.A., Bruns,G. and Georgopoulos,K.
  TITLE     The Ikaros gene encodes a family of lymphocyte-restricted zinc
            finger DNA binding proteins, highly conserved in human and mouse
  JOURNAL   J Immunol 156 (2), 585-592 (1996)
   PUBMED   8543809
REFERENCE   9  (bases 1 to 6255)
  AUTHORS   Hahm,K., Ernst,P., Lo,K., Kim,G.S., Turck,C. and Smale,S.T.
  TITLE     The lymphoid transcription factor LyF-1 is encoded by specific,
            alternatively spliced mRNAs derived from the Ikaros gene
  JOURNAL   Mol Cell Biol 14 (11), 7111-7123 (1994)
   PUBMED   7935426
REFERENCE   10 (bases 1 to 6255)
  AUTHORS   Georgopoulos,K., Moore,D.D. and Derfler,B.
  TITLE     Ikaros, an early lymphoid-specific transcription factor and a
            putative mediator for T cell commitment
  JOURNAL   Science 258 (5083), 808-812 (1992)
   PUBMED   1439790
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM148203.1, U40462.1 and
            JX459579.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 10, 2018 this sequence version replaced NM_006060.5.
            
            Summary: This gene encodes a transcription factor that belongs to
            the family of zinc-finger DNA-binding proteins associated with
            chromatin remodeling. The expression of this protein is restricted
            to the fetal and adult hemo-lymphopoietic system, and it functions
            as a regulator of lymphocyte differentiation. Several alternatively
            spliced transcript variants encoding different isoforms have been
            described for this gene. Most isoforms share a common C-terminal
            domain, which contains two zinc finger motifs that are required for
            hetero- or homo-dimerization, and for interactions with other
            proteins. The isoforms, however, differ in the number of N-terminal
            zinc finger motifs that bind DNA and in nuclear localization signal
            presence, resulting in members with and without DNA-binding
            properties. Only a few isoforms contain the requisite three or more
            N-terminal zinc motifs that confer high affinity binding to a
            specific core DNA sequence element in the promoters of target
            genes. The non-DNA-binding isoforms are largely found in the
            cytoplasm, and are thought to function as dominant-negative
            factors. Overexpression of some dominant-negative isoforms have
            been associated with B-cell malignancies, such as acute
            lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014].
            
            Transcript Variant: This variant (1) encodes isoform 1 (also known
            as Ik-1 as described in PMID:12937159). This isoform contains four
            N-terminal zinc finger motifs, binds DNA, and is localized to the
            nucleus.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.403115.1,
                                           SRR14038196.2982020.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000331340.8/ ENSP00000331614.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-163               BM148203.1         40-202
            164-1402            U40462.1           111-1349
            1403-6255           JX459579.1         597-5449
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p12.2"
     gene            1..6255
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /note="IKAROS family zinc finger 1"
                     /db_xref="GeneID:10320"
                     /db_xref="HGNC:HGNC:13176"
                     /db_xref="MIM:603023"
     exon            1..207
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /inference="alignment:Splign:2.1.0"
     exon            208..261
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /inference="alignment:Splign:2.1.0"
     CDS             222..1781
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /note="isoform 1 is encoded by transcript variant 1; zinc
                     finger protein, subfamily 1A, 1 (Ikaros); DNA-binding
                     protein Ikaros; CLL-associated antigen KW-6; ikaros family
                     zinc finger protein 1; protein phosphatase 1, regulatory
                     subunit 92; lymphoid transcription factor LyF-1"
                     /codon_start=1
                     /product="DNA-binding protein Ikaros isoform 1"
                     /protein_id="NP_006051.1"
                     /db_xref="CCDS:CCDS75596.1"
                     /db_xref="GeneID:10320"
                     /db_xref="HGNC:HGNC:13176"
                     /db_xref="MIM:603023"
                     /translation="MDADEGQDMSQVSGKESPPVSDTPDEGDEPMPIPEDLSTTSGGQ
                     QSSKSDRVVASNVKVETQSDEENGRACEMNGEECAEDLRMLDASGEKMNGSHRDQGSS
                     ALSGVGGIRLPNGKLKCDICGIICIGPNVLMVHKRSHTGERPFQCNQCGASFTQKGNL
                     LRHIKLHSGEKPFKCHLCNYACRRRDALTGHLRTHSVGKPHKCGYCGRSYKQRSSLEE
                     HKERCHNYLESMGLPGTLYPVIKEETNHSEMAEDLCKIGSERSLVLDRLASNVAKRKS
                     SMPQKFLGDKGLSDTPYDSSASYEKENEMMKSHVMDQAINNAINYLGAESLRPLVQTP
                     PGGSEVVPVISPMYQLHKPLAEGTPRSNHSAQDSAVENLLLLSKAKLVPSEREASPSN
                     SCQDSTDTESNNEEQRSGLIYLTNHIAPHARNGLSLKEEHRAYDLLRAASENSQDALR
                     VVSTSGEQMKVYKCEHCRVLFLDHVMYTIHMGCHGFRDPFECNMCGYHSQDRYEFSSH
                     ITRGEHRFHMS"
     exon            262..381
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /inference="alignment:Splign:2.1.0"
     exon            382..642
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /inference="alignment:Splign:2.1.0"
     exon            643..810
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /inference="alignment:Splign:2.1.0"
     exon            811..936
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1071
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..6255
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5572..5577
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /note="hexamer: AATAAA"
     polyA_site      5591
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /note="major polyA site"
     regulatory      6219..6224
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
                     /note="hexamer: AATAAA"
     polyA_site      6255
                     /gene="IKZF1"
                     /gene_synonym="CVID13; Hs.54452; IK1; IKAROS; LyF-1; LYF1;
                     PPP1R92; PRO0758; ZNFN1A1"
ORIGIN      
        1 actctaacaa gtgactgcgc ggcccgcgcc cggggcggtg actgcggcaa gccccctggg
       61 tccccgcgcg gcgcatccca gcctgggcgg gacgctcggc cgcggcgagg cgggcaagcc
      121 tggcagggca gagggagccc cggctccgag gttgctcttc gcacccgagg atcagtcttg
      181 gccccaaagc gcgacgcaca aatccacata acctgaggac catggatgct gatgagggtc
      241 aagacatgtc ccaagtttca gggaaggaaa gcccccctgt aagcgatact ccagatgagg
      301 gcgatgagcc catgccgatc cccgaggacc tctccaccac ctcgggagga cagcaaagct
      361 ccaagagtga cagagtcgtg gccagtaatg ttaaagtaga gactcagagt gatgaagaga
      421 atgggcgtgc ctgtgaaatg aatggggaag aatgtgcgga ggatttacga atgcttgatg
      481 cctcgggaga gaaaatgaat ggctcccaca gggaccaagg cagctcggct ttgtcgggag
      541 ttggaggcat tcgacttcct aacggaaaac taaagtgtga tatctgtggg atcatttgca
      601 tcgggcccaa tgtgctcatg gttcacaaaa gaagccacac tggagaacgg cccttccagt
      661 gcaatcagtg cggggcctca ttcacccaga agggcaacct gctccggcac atcaagctgc
      721 attccgggga gaagcccttc aaatgccacc tctgcaacta cgcctgccgc cggagggacg
      781 ccctcactgg ccacctgagg acgcactccg ttggtaaacc tcacaaatgt ggatattgtg
      841 gccgaagcta taaacagcga agctctttag aggaacataa agagcgctgc cacaactact
      901 tggaaagcat gggccttccg ggcacactgt acccagtcat taaagaagaa actaatcaca
      961 gtgaaatggc agaagacctg tgcaagatag gatcagagag atctctcgtg ctggacagac
     1021 tagcaagtaa cgtcgccaaa cgtaagagct ctatgcctca gaaatttctt ggggacaagg
     1081 gcctgtccga cacgccctac gacagcagcg ccagctacga gaaggagaac gaaatgatga
     1141 agtcccacgt gatggaccaa gccatcaaca acgccatcaa ctacctgggg gccgagtccc
     1201 tgcgcccgct ggtgcagacg cccccgggcg gttccgaggt ggtcccggtc atcagcccga
     1261 tgtaccagct gcacaagccg ctcgcggagg gcaccccgcg ctccaaccac tcggcccagg
     1321 acagcgccgt ggagaacctg ctgctgctct ccaaggccaa gttggtgccc tcggagcgcg
     1381 aggcgtcccc gagcaacagc tgccaagact ccacggacac cgagagcaac aacgaggagc
     1441 agcgcagcgg tctcatctac ctgaccaacc acatcgcccc gcacgcgcgc aacgggctgt
     1501 cgctcaagga ggagcaccgc gcctacgacc tgctgcgcgc cgcctccgag aactcgcagg
     1561 acgcgctccg cgtggtcagc accagcgggg agcagatgaa ggtgtacaag tgcgaacact
     1621 gccgggtgct cttcctggat cacgtcatgt acaccatcca catgggctgc cacggcttcc
     1681 gtgatccttt tgagtgcaac atgtgcggct accacagcca ggaccggtac gagttctcgt
     1741 cgcacataac gcgaggggag caccgcttcc acatgagcta aagccctccc gcgcccccac
     1801 cccagacccc gagccacccc aggaaaagca caaggactgc cgccttctcg ctcccgccag
     1861 cagcatagac tggactggac cagacaatgt tgtgtttgga tttgtaactg ttttttgttt
     1921 tttgtttgag ttggttgatt ggggtttgat ttgcttttga aaagattttt atttttagag
     1981 gcagggctgc attgggagca tccagaactg ctaccttcct agatgtttcc ccagaccgct
     2041 ggctgagatt ccctcacctg tcgcttccta gaatcccctt ctccaaacga ttagtctaaa
     2101 ttttcagaga gaaatagata aaacacgcca cagcctggga aggagcgtgc tctaccctgt
     2161 gctaagcacg gggttcgcgc accaggtgtc tttttccagt ccccagaagc agagagcaca
     2221 gcccctgctg tgtgggtctg caggtgagca gacaggacag gtgtgccgcc acccaagtgc
     2281 caagacacag cagggccaac aacctgtgcc caggccagct tcgagctaca tgcatctagg
     2341 gcggagaggc tgcacttgtg agagaaaata ctatttcaag tcatattctg cgtaggaaaa
     2401 tgaattggtt ggggaaagtc gtgtctgtca gactgccctg ggtggaggga gacgccgggc
     2461 tagagccttt gggatcgtcc tggattcact ggctttgcgg aggctgctca gatggcctga
     2521 gcctcccgag gcttgctgcc ccgtaggagg agactgtctt cccgtgggca tatctgggga
     2581 gccctgttcc ccgctttttc actcccatac ctttaatggc ccccaaaatc tgtcactaca
     2641 atttaaacac cagtcccgaa atttggatct tctttctttt tgaatctctc aaacggcaac
     2701 attcctcaga aaccaaagct ttatttcaaa tctcttcctt ccctggctgg ttccatctag
     2761 taccagaggc ctcttttcct gaagaaatcc aatcctagcc ctcattttaa ttatgtacat
     2821 ctgtttgtag ccacaagcct gaatttctca gtgttggtaa gtttctttac ctaccctcac
     2881 tatatattat tctcgtttta aaacccataa aggagtgatt tagaacagtc attaattttc
     2941 aactcaatga aatatgtgaa gcccagcatc tctgttgcta acacacagag ctcacctgtt
     3001 tgaaaccaag ctttcaaaca tgttgaagct ctttactgta aaggcaagcc agcatgtgtg
     3061 tccacacata cataggatgg ctggctctgc acctgtagga tattggaatg cacagggcaa
     3121 ttgagggact gagccagacc ttcggagagt aatgccacca gatcccctag gaaagaggag
     3181 gcaaatggca ctgcaggtga gaaccccgcc catccgtgct atgacatgga ggcactgaag
     3241 cccgaggaag gtgtgtggag attctaatcc caacaagcaa gggtctcctt caagattaat
     3301 gctatcaatc attaaggtca ttactctcaa ccacctaggc aatgaagaat ataccatttc
     3361 aaatatttac agtacttgtc ttcaccaaca ctgtcccaag gtgaaatgaa gcaacagaga
     3421 ggaaattgta cataagtacc tcagcattta atccaaacag gggttcttag tctcagcact
     3481 atgacatttt gggctgacta cttatttgtt aggcgggagc tctcctgtgc attgtaggat
     3541 aattagcagt atccctggtg gctacccaat agacgccagt agcaccccga attgacaacc
     3601 caaactctcc agacatcacc aactgtcccc tgcgaggaga aatcactcct gggggagaac
     3661 cactgaccca aatgaattct aaaccaatca aatgtctggg aagccctcca agaaaaaaaa
     3721 tagaaaagca cttgaagaat attcccaata ttcccggtca gcagtatcaa ggctgacttg
     3781 tgttcatgtg gagtcattat aaattctata aatcaattat tccccttcgg tcttaaaaat
     3841 atatttcctc ataaacattt gagttttgtt gaaaagatgg agtttacaaa gataccattc
     3901 ttgagtcatg gatttctctg ctcacagaag ggtgtggcat ttggaaacgg gaataaacaa
     3961 aattgctgca ccaatgcact gagtgaagga agagagacag aggatcaagg gctttagaca
     4021 gcactccttc aatatgcaat cacagagaaa gatgcgcctt atccaagtta atatctctaa
     4081 ggtgagagcc ttcttagagt cagtttgttg caaatttcac ctactctgtt cttttccatc
     4141 catccccctg agtcagttgg ttgaagggag ttattttttc aagtggaatt caaacaaagc
     4201 tcaaaccaga actgtaaata gtgattgcag gaattctttt ctaaactgct ttgccctttc
     4261 ctctcactgc cttttatagc caatataaat gtctctttgc acaccttttg ttgtggtttt
     4321 atattgtaac accatttttc tttgaaacta ttgtatttaa agtaaggttt catattatgt
     4381 cagcaagtaa ttaacttatg tttaaaaggt ggccatatca tgtaccaaaa gttgctgaag
     4441 tttctcttct agctggtaaa gtaggagttt gcatgacttc acactttttt tgcgtagttt
     4501 cttctgttgt atgatggcgt gagtgtgtgt cttgggtacc gctgtgtact actgtgtgcc
     4561 tagattccat gcactctcgt tgtgtttgaa gtaaatattg gagaccggag ggtaacaggt
     4621 tggcctgttg attacagcta gtaatcgctg tgtcttgttc cgccccctcc ctgacacccc
     4681 agcttcccag gatgtggaaa gcctggatct cagctccttg ccccatatcc cttctgtaat
     4741 ttgtacctaa agagtgtgat tatcctaatt caagagtcac taaaactcat cacattatca
     4801 ttgcatatca gcaaagggta aagtcctagc accaattgct tcacatacca gcatgttcca
     4861 tttccaattt agaattagcc acataataaa atcttagaat cttccttgag aaagagctgc
     4921 ctgagatgta gttttgttat atggttcccc accgaccatt tttgtgcttt tttcttgttt
     4981 tgttttgttt tgactgcact gtgagttttg tagtgtcctc ttcttgccaa aacaaacgcg
     5041 agatgaactg gacttatgta gacaaatcgt gatgccagtg tatccttcct ttcttcagtt
     5101 ccagcaataa tgaatggtca acttttttaa aatctagatc tctctcattc atttcaatgt
     5161 atttttactt taagatgaac caaaattatt agacttattt aagatgtaca ggcatcagaa
     5221 aaaagaagca cataatgctt ttggtgcgat ggcactcact gtgaacatgt gtaaccacat
     5281 attaatatgc aatattgttt ccaatacttt ctaatacagt tttttataat gttgtgtgtg
     5341 gtgattgttc aggtcgaatc tgttgtatcc agtacagctt taggtcttca gctgcccttc
     5401 tggcgagtac atgcacagga ttgtaaatga gaaatgcagt catatttcca gtctgcctct
     5461 atgatgatgt taaattattg ctgtttagct gtgaacaagg gatgtaccac tggaggaata
     5521 gagtatcctt ttgtacacat tttgaaatgc ttcttctgta gtgatagaac aaataaatgc
     5581 aacgaatact ctgtctgccc tatcccgtga agtccacact ggcgtaagag aaggcccagc
     5641 agagcaggaa tctgcctaga ctttctccca atgagatccc aatatgagag ggagaagaga
     5701 tgggcctcag gacagctgca ataccacttg ggaacacatg tggtgtcttg atgtggccag
     5761 cgcagcagtt cagcacaacg tacctcccat ctacaacagt gctggacgtg ggaattctaa
     5821 gtcccagtct tgagggtggg tggagatgga gggcaacaag agatacattt ccagttctcc
     5881 actgcagcat gcttcagtca ttctgtgagt ggccgggccc agggccctca caatttcact
     5941 accttgtctt ttacatagtc ataagaatta tcctcaacat agccttttga cgctgtaaat
     6001 cttgagtatt catttaccct tttctgatct cctggaaaca gctgcctgcc tgcattgcac
     6061 ttctcttccc gaggagtggg gtaaatttaa aagtcaagtt atagtttgga tgttagtata
     6121 gaattttgaa attgggaatt aaaaatcagg actggggact gggagaccaa aaatttctga
     6181 tcccatttct gatggatgtg tcacaccttt tctgtcaaaa taaaatgtct tggaggttat
     6241 gactccttgg tgaaa
//
