LOCUS       NM_001135197            2700 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens interactor of HORMAD1 1 (IHO1), transcript variant 2,
            mRNA.
ACCESSION   NM_001135197
VERSION     NM_001135197.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2700)
  AUTHORS   Wang,W., Dong,J., Chen,B., Du,J., Kuang,Y., Sun,X., Fu,J., Li,B.,
            Mu,J., Zhang,Z., Zhou,Z., Lin,Z., Wu,L., Yan,Z., Mao,X., Li,Q.,
            He,L., Wang,L. and Sang,Q.
  TITLE     Homozygous mutations in REC114 cause female infertility
            characterised by multiple pronuclei formation and early embryonic
            arrest
  JOURNAL   J Med Genet 57 (3), 187-194 (2020)
   PUBMED   31704776
REFERENCE   4  (bases 1 to 2700)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2700)
  AUTHORS   Chen,S., Fragoza,R., Klei,L., Liu,Y., Wang,J., Roeder,K., Devlin,B.
            and Yu,H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   6  (bases 1 to 2700)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   7  (bases 1 to 2700)
  AUTHORS   Sahni,N., Yi,S., Taipale,M., Fuxman Bass,J.I.,
            Coulombe-Huntington,J., Yang,F., Peng,J., Weile,J., Karras,G.I.,
            Wang,Y., Kovacs,I.A., Kamburov,A., Krykbaeva,I., Lam,M.H.,
            Tucker,G., Khurana,V., Sharma,A., Liu,Y.Y., Yachie,N., Zhong,Q.,
            Shen,Y., Palagi,A., San-Miguel,A., Fan,C., Balcha,D., Dricot,A.,
            Jordan,D.M., Walsh,J.M., Shah,A.A., Yang,X., Stoyanova,A.K.,
            Leighton,A., Calderwood,M.A., Jacob,Y., Cusick,M.E.,
            Salehi-Ashtiani,K., Whitesell,L.J., Sunyaev,S., Berger,B.,
            Barabasi,A.L., Charloteaux,B., Hill,D.E., Hao,T., Roth,F.P.,
            Xia,Y., Walhout,A.J.M., Lindquist,S. and Vidal,M.
  TITLE     Widespread macromolecular interaction perturbations in human
            genetic disorders
  JOURNAL   Cell 161 (3), 647-660 (2015)
   PUBMED   25910212
REFERENCE   8  (bases 1 to 2700)
  AUTHORS   Corominas,R., Yang,X., Lin,G.N., Kang,S., Shen,Y., Ghamsari,L.,
            Broly,M., Rodriguez,M., Tam,S., Wanamaker,S.A., Fan,C., Yi,S.,
            Tasan,M., Lemmens,I., Kuang,X., Zhao,N., Malhotra,D.,
            Michaelson,J.J., Vacic,V., Calderwood,M.A., Roth,F.P.,
            Tavernier,J., Horvath,S., Salehi-Ashtiani,K., Korkin,D., Sebat,J.,
            Hill,D.E., Hao,T., Vidal,M. and Iakoucheva,L.M.
  TITLE     Protein interaction network of alternatively spliced isoforms from
            brain links genetic risk factors for autism
  JOURNAL   Nat Commun 5, 3650 (2014)
   PUBMED   24722188
  REMARK    Erratum:[Nat Commun. 2023 Feb 2;14(1):569. doi:
            10.1038/s41467-023-36264-y.. PMID: 36732511]
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 2700)
  AUTHORS   Orvedahl,A., Sumpter,R. Jr., Xiao,G., Ng,A., Zou,Z., Tang,Y.,
            Narimatsu,M., Gilpin,C., Sun,Q., Roth,M., Forst,C.V., Wrana,J.L.,
            Zhang,Y.E., Luby-Phelps,K., Xavier,R.J., Xie,Y. and Levine,B.
  TITLE     Image-based genome-wide siRNA screen identifies selective autophagy
            factors
  JOURNAL   Nature 480 (7375), 113-117 (2011)
   PUBMED   22020285
REFERENCE   10 (bases 1 to 2700)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC135506.3, AK303033.1, BC036202.1, AA858032.1 and AC121247.2.
            
            On Nov 23, 2018 this sequence version replaced NM_001135197.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK303033.1, SRR5189667.154219.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2142680
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000452691.7/ ENSP00000407837.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AC135506.3         129799-129800
            3-162               AK303033.1         1-160
            163-2317            BC036202.1         459-2613
            2318-2698           AA858032.1         1-381               c
            2699-2700           AC121247.2         122795-122796       c
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..2700
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /note="interactor of HORMAD1 1"
                     /db_xref="GeneID:339834"
                     /db_xref="HGNC:HGNC:27945"
                     /db_xref="MIM:619190"
     exon            1..76
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /inference="alignment:Splign:2.1.0"
     exon            77..147
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..1876
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /note="cancer/testis antigen 74; coiled-coil
                     domain-containing protein 36; coiled-coil domain
                     containing 36"
                     /codon_start=1
                     /product="interactor of HORMAD1 protein 1"
                     /protein_id="NP_001128669.1"
                     /db_xref="CCDS:CCDS33755.2"
                     /db_xref="GeneID:339834"
                     /db_xref="HGNC:HGNC:27945"
                     /db_xref="MIM:619190"
                     /translation="MNFNVWNIKEMLSIPSGSGNKKSSNWNNNQNDYSSLSDSQFLFG
                     SQFCPENSETLSAPLDFGAHLRHSKQSQQNYLEGEPSIFTKYQTKPQLFGGDIKDGGL
                     FPPPLSVGKSKGLLEQFEEKKKRAKDKCDSETLYNFVSNVRESILRLQTSVEKSEDHL
                     SSRSQSILDSLETVAKTLQETIQAQNDLVFEAVQDKGNMEQAILEMKKRFEARQGEFI
                     EMKSNLKHLEVLVAQQSQEFQQLCEQLGQLNVPSVLAELKRLISVPPVKDSASQTSPP
                     LAQSLNLTRQEKYTSEKPVLWQAQALPAAWNPGMGSLQPGEFDVWGEGAKNDDLQEEA
                     ALPAFGSHERNRHVKDKVVQTNCKNWAVTKTGAKNHGSSVPGHKIPSDRDLVSQGASQ
                     LTSLEINFSTSIKNACQKYQAQSMFLCDPREHLVIKQKDGTVEMRGKDKKQQPRKAHR
                     AHRGRLIASKQKQIPIQTCKFNSKYQSPQPAISVPQSPFLGQQEPRAQPLHLQCPRSP
                     RKPVCPILGGTVMPNKTVRAVQGRLLQLSRCSSQDNWLLSSSSQGDHQMSWFSDLNLG
                     CSETPLCKEAGKNLLYDLGFDSSDDDGF"
     exon            148..322
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /inference="alignment:Splign:2.1.0"
     exon            323..486
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /inference="alignment:Splign:2.1.0"
     exon            487..535
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /inference="alignment:Splign:2.1.0"
     exon            536..623
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /inference="alignment:Splign:2.1.0"
     exon            624..727
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /inference="alignment:Splign:2.1.0"
     exon            728..2700
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2422..2427
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /note="hexamer: AATAAA"
     polyA_site      2448
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /note="major polyA site"
     regulatory      2682..2687
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /note="hexamer: GATAAA"
     polyA_site      2700
                     /gene="IHO1"
                     /gene_synonym="CCDC36; CT74; LELA1"
                     /note="major polyA site"
ORIGIN      
        1 gcagaggcag cgggacgcgg ccacctcgaa gccacgtcag ggcagcccca ggtcgggcgc
       61 gtgccagcag ccagaggtat aactataaga aatgaatttt aatgtctgga atatcaaaga
      121 gatgctcagt attccttcag gctctgggaa caagaagtca tccaactgga ataataatca
      181 aaatgattat tccagtctca gtgattccca gttcctcttt ggatctcagt tctgtccaga
      241 aaattcagaa accctatcag cacccttgga ctttggtgcc cacttgagac attcaaaaca
      301 gtcacaacag aactatctgg agggtgaacc tagcattttc acaaagtacc agacaaagcc
      361 ccagctgttc ggaggagata taaaagatgg aggtttattt cctcctcctt tgtcagttgg
      421 aaaatcaaaa ggcctcttgg aacagtttga ggagaaaaag aaaagggcaa aagacaaatg
      481 tgacagtgag actctataca actttgtttc taatgttaga gaaagcattc tcaggttgca
      541 gacgtctgtg gaaaagtctg aggaccatct cagttcaaga agccaatcta ttttggattc
      601 tttggagact gtggccaaga cattacaaga gactatacag gcccagaatg acctggtgtt
      661 tgaggcagtc caggacaaag gcaacatgga gcaggccatc cttgagatga agaaaagatt
      721 tgaagctaga caaggagagt ttatagaaat gaagtccaac ctgaagcacc ttgaagtttt
      781 agttgctcag cagagtcagg aattccagca gctgtgtgag cagctaggcc agctgaatgt
      841 gcccagtgtc ctagcagagc tgaagagatt gatctcagtg cctccagtga aagacagtgc
      901 ttctcagacg tcgccacctt tggcccagag cctcaatctc accaggcagg aaaaatacac
      961 ctctgagaaa ccagttttat ggcaggccca ggccctccct gctgcatgga atcctggtat
     1021 gggctcccta cagcctggag aatttgatgt ctggggtgaa ggagcaaaga atgatgatct
     1081 ccaagaagag gctgcactgc cagcatttgg gtcccatgaa agaaataggc atgtaaagga
     1141 caaggtggtg cagactaact gcaagaactg ggctgttact aaaacaggtg ccaagaacca
     1201 tggttccagc gtcccaggcc ataagattcc cagtgacagg gacctggttt cccaaggagc
     1261 ctcacagctc acatcattgg agataaactt ttcaaccagc attaagaatg cctgccaaaa
     1321 atatcaagcc caaagtatgt ttttgtgtga cccacgtgaa catttggtga ttaaacagaa
     1381 agatgggact gtagaaatgc gggggaaaga caagaagcag cagcccagga aggcccacag
     1441 ggcccacaga ggcaggctca tagccagcaa gcaaaaacaa atcccaatcc agacctgtaa
     1501 attcaattcc aaatatcaga gtcctcagcc tgcaatttct gtccctcaaa gccccttcct
     1561 ggggcagcag gaaccccgtg ctcagcctct gcatctgcag tgtcccagga gccccagaaa
     1621 accagtctgc cctattctgg gaggaacagt catgcccaat aagacagtaa gggcagtgca
     1681 gggaagactc ttgcagctca gcaggtgctc ttcccaagac aactggctac tttccagcag
     1741 ttcccagggg gaccaccaga tgagctggtt cagtgacctc aatctcggat gttcagagac
     1801 ccctctatgc aaggaggcag gaaagaattt gctctatgac ctgggttttg atagcagtga
     1861 tgatgatggc ttctgaccag tccacagttg atttattggt ctcagctaga aagagaaatt
     1921 gcaggacatt tgggctggcc aacagcagaa agtccctgaa gcctgccaag tacccagggt
     1981 cagaggccct gctgaggtgg ggcaatgagc acgagggcag ggaaggtcca gcattctggg
     2041 taccaggtgc tgcccctgac aaggattaag tgcatcctta tttattggaa tgaaatgtga
     2101 aaatggtgct gatgaaaact gaggcagcag cctggttgtg gggcatctgg agcagggtgc
     2161 ctgtactttg gcgaaaggca ggcaggcctc cttatggaat actgtgtagc agaagggctc
     2221 tcataaaact gttgcggtgg cagcatgact ccaaacaaca tttttgggtc tttaaaatat
     2281 tccctacaca tgaccaatac attttcaggc ctcaggattt ttttctttct tatttttagc
     2341 cacttatgct accagaatgt ttgcattttt tgatcagtag acatattaca ggacacccta
     2401 aatcttcttg tgaacagaga aaataaaatg ataagcctgc cagtgaaaga ggtgacattc
     2461 actagcaaat gtccagagca tatcactgct cacaagccat ttttcttcct ggttgctttt
     2521 tcccttgtcc tgtttttcaa gcactttctt aaaactaata atattgtaga ccttgtattt
     2581 ctgaagagca gtgggcttat atgggacttt ggctttccat ttttatcttg ctgaagtttt
     2641 tgagtttttg caacacaagt attttttgtt atcagaaata agataaaaca tttaaaagca
//
