LOCUS       NM_052868               2270 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens immunoglobulin superfamily member 8 (IGSF8),
            transcript variant 1, mRNA.
ACCESSION   NM_052868
VERSION     NM_052868.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Li,Y., Wu,X., Sheng,C., Liu,H., Liu,H., Tang,Y., Liu,C., Ding,Q.,
            Xie,B., Xiao,X., Zheng,R., Yu,Q., Guo,Z., Ma,J., Wang,J., Gao,J.,
            Tian,M., Wang,W., Zhou,J., Jiang,L., Gu,M., Shi,S., Paull,M.,
            Yang,G., Yang,W., Landau,S., Bao,X., Hu,X., Liu,X.S. and Xiao,T.
  TITLE     IGSF8 is an innate immune checkpoint and cancer immunotherapy
            target
  JOURNAL   Cell 187 (11), 2703-2716 (2024)
   PUBMED   38657602
  REMARK    GeneRIF: IGSF8 is an innate immune checkpoint and cancer
            immunotherapy target.
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Ding,Y., Chen,J., Li,S., Wren,J.D., Bajpai,A.K., Wang,J.,
            Tanaka,T., Rice,H.C., Hays,F.A., Lu,L. and Zhang,X.A.
  TITLE     EWI2 and its relatives in Tetraspanin-enriched membrane domains
            regulate malignancy
  JOURNAL   Oncogene 42 (12), 861-868 (2023)
   PUBMED   36788350
  REMARK    GeneRIF: EWI2 and its relatives in Tetraspanin-enriched membrane
            domains regulate malignancy.
            Review article
REFERENCE   3  (bases 1 to 2270)
  AUTHORS   Fu,C., Wang,J., Pallikkuth,S., Ding,Y., Chen,J., Wren,J.D.,
            Yang,Y., Wong,K.K., Kameyama,H., Jayaraman,M., Munshi,A.,
            Tanaka,T., Lidke,K.A. and Zhang,X.A.
  TITLE     EWI2 prevents EGFR from clustering and endocytosis to reduce tumor
            cell movement and proliferation
  JOURNAL   Cell Mol Life Sci 79 (7), 389 (2022)
   PUBMED   35773608
  REMARK    GeneRIF: EWI2 prevents EGFR from clustering and endocytosis to
            reduce tumor cell movement and proliferation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2270)
  AUTHORS   Wang,J., Wren,J.D., Ding,Y., Chen,J., Mittal,N., Xu,C., Li,X.,
            Zeng,C., Wang,M., Shi,J., Zhang,Y.H., Han,S.J. and Zhang,X.A.
  TITLE     EWI2 promotes endolysosome-mediated turnover of growth factor
            receptors and integrins to suppress lung cancer
  JOURNAL   Cancer Lett 536, 215641 (2022)
   PUBMED   35339615
  REMARK    GeneRIF: EWI2 promotes endolysosome-mediated turnover of growth
            factor receptors and integrins to suppress lung cancer.
REFERENCE   5  (bases 1 to 2270)
  AUTHORS   Kolesnikova,T.V., Stipp,C.S., Rao,R.M., Lane,W.S., Luscinskas,F.W.
            and Hemler,M.E.
  TITLE     EWI-2 modulates lymphocyte integrin alpha4beta1 functions
  JOURNAL   Blood 103 (8), 3013-3019 (2004)
   PUBMED   15070678
  REMARK    GeneRIF: EWI-2-dependent reorganization of alpha4beta1-CD81
            complexes on the cell surface is responsible for EWI-2 effects on
            integrin-dependent morphology and motility functions
REFERENCE   6  (bases 1 to 2270)
  AUTHORS   Stipp,C.S., Kolesnikova,T.V. and Hemler,M.E.
  TITLE     EWI-2 regulates alpha3beta1 integrin-dependent cell functions on
            laminin-5
  JOURNAL   J Cell Biol 163 (5), 1167-1177 (2003)
   PUBMED   14662754
REFERENCE   7  (bases 1 to 2270)
  AUTHORS   Charrin,S., Le Naour,F., Labas,V., Billard,M., Le Caer,J.P.,
            Emile,J.F., Petit,M.A., Boucheix,C. and Rubinstein,E.
  TITLE     EWI-2 is a new component of the tetraspanin web in hepatocytes and
            lymphoid cells
  JOURNAL   Biochem J 373 (Pt 2), 409-421 (2003)
   PUBMED   12708969
REFERENCE   8  (bases 1 to 2270)
  AUTHORS   Zhang,X.A., Lane,W.S., Charrin,S., Rubinstein,E. and Liu,L.
  TITLE     EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and
            inhibits the migration of prostate cancer cells
  JOURNAL   Cancer Res 63 (10), 2665-2674 (2003)
   PUBMED   12750295
  REMARK    GeneRIF: EWI2/PGRL associates with the metastasis suppressor
            KAI1/CD82 and inhibits the migration of prostate cancer cells.
REFERENCE   9  (bases 1 to 2270)
  AUTHORS   Stipp,C.S., Kolesnikova,T.V. and Hemler,M.E.
  TITLE     EWI-2 is a major CD9 and CD81 partner and member of a novel Ig
            protein subfamily
  JOURNAL   J Biol Chem 276 (44), 40545-40554 (2001)
   PUBMED   11504738
REFERENCE   10 (bases 1 to 2270)
  AUTHORS   Clark,K.L., Zeng,Z., Langford,A.L., Bowen,S.M. and Todd,S.C.
  TITLE     PGRL is a major CD81-associated protein on lymphocytes and
            distinguishes a new family of cell surface proteins
  JOURNAL   J Immunol 167 (9), 5115-5121 (2001)
   PUBMED   11673522
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC053881.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_052868.5.
            
            Summary: This gene encodes a member the EWI subfamily of the
            immunoglobulin protein superfamily. Members of this family contain
            a single transmembrane domain, an EWI (Glu-Trp-Ile)-motif and a
            variable number of immunoglobulin domains. This protein interacts
            with the tetraspanins CD81 and CD9 and may regulate their role in
            certain cellular functions including cell migration and viral
            infection. The encoded protein may also function as a tumor
            suppressor by inhibiting the proliferation of certain cancers.
            Alternate splicing results in multiple transcript variants that
            encode the same protein. [provided by RefSeq, Sep 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2 and 3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053881.1, AF407274.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000314485.12/ ENSP00000316664.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2270              BC053881.1         17-2286
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q23.2"
     gene            1..2270
                     /gene="IGSF8"
                     /gene_synonym="CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1;
                     PGRL"
                     /note="immunoglobulin superfamily member 8"
                     /db_xref="GeneID:93185"
                     /db_xref="HGNC:HGNC:17813"
                     /db_xref="MIM:606644"
     exon            1..211
                     /gene="IGSF8"
                     /gene_synonym="CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1;
                     PGRL"
                     /inference="alignment:Splign:2.1.0"
     CDS             148..1989
                     /gene="IGSF8"
                     /gene_synonym="CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1;
                     PGRL"
                     /note="CD81 partner 3; glu-Trp-Ile EWI motif-containing
                     protein 2; keratinocytes-associated transmembrane protein
                     4; prostaglandin regulatory-like protein"
                     /codon_start=1
                     /product="immunoglobulin superfamily member 8 precursor"
                     /protein_id="NP_443100.1"
                     /db_xref="CCDS:CCDS1195.1"
                     /db_xref="GeneID:93185"
                     /db_xref="HGNC:HGNC:17813"
                     /db_xref="MIM:606644"
                     /translation="MGALRPTLLPPSLPLLLLLMLGMGCWAREVLVPEGPLYRVAGTA
                     VSISCNVTGYEGPAQQNFEWFLYRPEAPDTALGIVSTKDTQFSYAVFKSRVVAGEVQV
                     QRLQGDAVVLKIARLQAQDAGIYECHTPSTDTRYLGSYSGKVELRVLPDVLQVSAAPP
                     GPRGRQAPTSPPRMTVHEGQELALGCLARTSTQKHTHLAVSFGRSVPEAPVGRSTLQE
                     VVGIRSDLAVEAGAPYAERLAAGELRLGKEGTDRYRMVVGGAQAGDAGTYHCTAAEWI
                     QDPDGSWAQIAEKRAVLAHVDVQTLSSQLAVTVGPGERRIGPGEPLELLCNVSGALPP
                     AGRHAAYSVGWEMAPAGAPGPGRLVAQLDTEGVGSLGPGYEGRHIAMEKVASRTYRLR
                     LEAARPGDAGTYRCLAKAYVRGSGTRLREAASARSRPLPVHVREEGVVLEAVAWLAGG
                     TVYRGETASLLCNISVRGGPPGLRLAASWWVERPEDGELSSVPAQLVGGVGQDGVAEL
                     GVRPGGGPVSVELVGPRSHRLRLHSLGPEDEGVYHCAPSAWVQHADYSWYQAGSARSG
                     PVTVYPYMHALDTLFVPLLVGTGVALVTGATVLGTITCCFMKRLRKR"
     exon            212..589
                     /gene="IGSF8"
                     /gene_synonym="CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1;
                     PGRL"
                     /inference="alignment:Splign:2.1.0"
     exon            590..1051
                     /gene="IGSF8"
                     /gene_synonym="CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1;
                     PGRL"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1459
                     /gene="IGSF8"
                     /gene_synonym="CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1;
                     PGRL"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1873
                     /gene="IGSF8"
                     /gene_synonym="CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1;
                     PGRL"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..2004
                     /gene="IGSF8"
                     /gene_synonym="CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1;
                     PGRL"
                     /inference="alignment:Splign:2.1.0"
     exon            2005..2270
                     /gene="IGSF8"
                     /gene_synonym="CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1;
                     PGRL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2250..2255
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IGSF8"
                     /gene_synonym="CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1;
                     PGRL"
                     /note="hexamer: AATAAA"
     polyA_site      2270
                     /gene="IGSF8"
                     /gene_synonym="CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1;
                     PGRL"
                     /note="major polyA site"
ORIGIN      
        1 attcggcagc tccagttcag ctcgctcggc gcacccacgc ctcgctgccc cgcttcctgc
       61 cctcaacctg ggcatgcgcc ccccaccctt ccggcccccc agaacccgcg ccatcccccg
      121 gagcctcccc agagctggcc gcgcaggatg ggcgccctca ggcccacgct gctgccgcct
      181 tcgctgccgc tgctgctgct gctaatgcta ggaatgggat gctgggcccg ggaggtgctg
      241 gtccccgagg ggcccttgta ccgcgtggct ggcacagctg tctccatctc ctgcaatgtg
      301 accggctatg agggccctgc ccagcagaac ttcgagtggt tcctgtatag gcccgaggcc
      361 ccagatactg cactgggcat tgtcagtacc aaggataccc agttctccta tgctgtcttc
      421 aagtcccgag tggtggcggg tgaggtgcag gtgcagcgcc tacaaggtga tgccgtggtg
      481 ctcaagattg cccgcctgca ggcccaggat gccggcattt atgagtgcca caccccctcc
      541 actgataccc gctacctggg cagctacagc ggcaaggtgg agctgagagt tcttccagat
      601 gtcctccagg tgtctgctgc ccccccaggg ccccgaggcc gccaggcccc aacctcaccc
      661 ccacgcatga cggtgcatga ggggcaggag ctggcactgg gctgcctggc gaggacaagc
      721 acacagaagc acacacacct ggcagtgtcc tttgggcgat ctgtgcccga ggcaccagtt
      781 gggcggtcaa ctctgcagga agtggtggga atccggtcag acttggccgt ggaggctgga
      841 gctccctatg ctgagcgatt ggctgcaggg gagcttcgtc tgggcaagga agggaccgat
      901 cggtaccgca tggtagtagg gggtgcccag gcaggggacg caggcaccta ccactgcact
      961 gccgctgagt ggattcagga tcctgatggc agctgggccc agattgcaga gaaaagggcc
     1021 gtcctggccc acgtggatgt gcagacgctg tccagccagc tggcagtgac agtggggcct
     1081 ggtgaacgtc ggatcggccc aggggagccc ttggaactgc tgtgcaatgt gtcaggggca
     1141 cttcccccag caggccgtca tgctgcatac tctgtaggtt gggagatggc acctgcgggg
     1201 gcacctgggc ccggccgcct ggtagcccag ctggacacag agggtgtggg cagcctgggc
     1261 cctggctatg agggccgaca cattgccatg gagaaggtgg catccagaac ataccggcta
     1321 cggctagagg ctgccaggcc tggtgatgcg ggcacctacc gctgcctcgc caaagcctat
     1381 gttcgagggt ctgggacccg gcttcgtgaa gcagccagtg cccgttcccg gcctctccct
     1441 gtacatgtgc gggaggaagg tgtggtgctg gaggctgtgg catggctagc aggaggcaca
     1501 gtgtaccgcg gggagactgc ctccctgctg tgcaacatct ctgtgcgggg tggcccccca
     1561 ggactgcggc tggccgccag ctggtgggtg gagcgaccag aggacggaga gctcagctct
     1621 gtccctgccc agctggtggg tggcgtaggc caggatggtg tggcagagct gggagtccgg
     1681 cctggaggag gccctgtcag cgtagagctg gtggggcccc gaagccatcg gctgagacta
     1741 cacagcttgg ggcccgagga tgaaggcgtg taccactgtg cccccagcgc ctgggtgcag
     1801 catgccgact acagctggta ccaggcgggc agtgcccgct cagggcctgt tacagtctac
     1861 ccctacatgc atgccctgga caccctattt gtgcctctgc tggtgggtac aggggtggcc
     1921 ctagtcactg gtgccactgt ccttggtacc atcacttgct gcttcatgaa gaggcttcga
     1981 aaacggtgat cccttactcc ccaggtcttg caggtgtcga ctgtcttccg gcccagctcc
     2041 aagccctcct ctggttgcct ggacaccctc tccctctgtc cactcttcct ttaatttatt
     2101 tgacctccca ctacccagaa tgggagacgt gcctcccctt ccccactcct tccctcccaa
     2161 gcccctccct ctggccttct gttcttgatc tcttagggat cctataggga ggccatttcc
     2221 tgtcctggaa ttagtttttc taaaatgtga ataaacttgt tttataaaaa
//
