LOCUS       NP_443100                613 aa            linear   PRI 08-JUN-2024
DEFINITION  immunoglobulin superfamily member 8 precursor [Homo sapiens].
ACCESSION   NP_443100
VERSION     NP_443100.1
DBSOURCE    REFSEQ: accession NM_052868.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 613)
  AUTHORS   Li,Y., Wu,X., Sheng,C., Liu,H., Liu,H., Tang,Y., Liu,C., Ding,Q.,
            Xie,B., Xiao,X., Zheng,R., Yu,Q., Guo,Z., Ma,J., Wang,J., Gao,J.,
            Tian,M., Wang,W., Zhou,J., Jiang,L., Gu,M., Shi,S., Paull,M.,
            Yang,G., Yang,W., Landau,S., Bao,X., Hu,X., Liu,X.S. and Xiao,T.
  TITLE     IGSF8 is an innate immune checkpoint and cancer immunotherapy
            target
  JOURNAL   Cell 187 (11), 2703-2716 (2024)
   PUBMED   38657602
  REMARK    GeneRIF: IGSF8 is an innate immune checkpoint and cancer
            immunotherapy target.
REFERENCE   2  (residues 1 to 613)
  AUTHORS   Ding,Y., Chen,J., Li,S., Wren,J.D., Bajpai,A.K., Wang,J.,
            Tanaka,T., Rice,H.C., Hays,F.A., Lu,L. and Zhang,X.A.
  TITLE     EWI2 and its relatives in Tetraspanin-enriched membrane domains
            regulate malignancy
  JOURNAL   Oncogene 42 (12), 861-868 (2023)
   PUBMED   36788350
  REMARK    GeneRIF: EWI2 and its relatives in Tetraspanin-enriched membrane
            domains regulate malignancy.
            Review article
REFERENCE   3  (residues 1 to 613)
  AUTHORS   Fu,C., Wang,J., Pallikkuth,S., Ding,Y., Chen,J., Wren,J.D.,
            Yang,Y., Wong,K.K., Kameyama,H., Jayaraman,M., Munshi,A.,
            Tanaka,T., Lidke,K.A. and Zhang,X.A.
  TITLE     EWI2 prevents EGFR from clustering and endocytosis to reduce tumor
            cell movement and proliferation
  JOURNAL   Cell Mol Life Sci 79 (7), 389 (2022)
   PUBMED   35773608
  REMARK    GeneRIF: EWI2 prevents EGFR from clustering and endocytosis to
            reduce tumor cell movement and proliferation.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 613)
  AUTHORS   Wang,J., Wren,J.D., Ding,Y., Chen,J., Mittal,N., Xu,C., Li,X.,
            Zeng,C., Wang,M., Shi,J., Zhang,Y.H., Han,S.J. and Zhang,X.A.
  TITLE     EWI2 promotes endolysosome-mediated turnover of growth factor
            receptors and integrins to suppress lung cancer
  JOURNAL   Cancer Lett 536, 215641 (2022)
   PUBMED   35339615
  REMARK    GeneRIF: EWI2 promotes endolysosome-mediated turnover of growth
            factor receptors and integrins to suppress lung cancer.
REFERENCE   5  (residues 1 to 613)
  AUTHORS   Kolesnikova,T.V., Stipp,C.S., Rao,R.M., Lane,W.S., Luscinskas,F.W.
            and Hemler,M.E.
  TITLE     EWI-2 modulates lymphocyte integrin alpha4beta1 functions
  JOURNAL   Blood 103 (8), 3013-3019 (2004)
   PUBMED   15070678
  REMARK    GeneRIF: EWI-2-dependent reorganization of alpha4beta1-CD81
            complexes on the cell surface is responsible for EWI-2 effects on
            integrin-dependent morphology and motility functions
REFERENCE   6  (residues 1 to 613)
  AUTHORS   Stipp,C.S., Kolesnikova,T.V. and Hemler,M.E.
  TITLE     EWI-2 regulates alpha3beta1 integrin-dependent cell functions on
            laminin-5
  JOURNAL   J Cell Biol 163 (5), 1167-1177 (2003)
   PUBMED   14662754
REFERENCE   7  (residues 1 to 613)
  AUTHORS   Charrin,S., Le Naour,F., Labas,V., Billard,M., Le Caer,J.P.,
            Emile,J.F., Petit,M.A., Boucheix,C. and Rubinstein,E.
  TITLE     EWI-2 is a new component of the tetraspanin web in hepatocytes and
            lymphoid cells
  JOURNAL   Biochem J 373 (Pt 2), 409-421 (2003)
   PUBMED   12708969
REFERENCE   8  (residues 1 to 613)
  AUTHORS   Zhang,X.A., Lane,W.S., Charrin,S., Rubinstein,E. and Liu,L.
  TITLE     EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and
            inhibits the migration of prostate cancer cells
  JOURNAL   Cancer Res 63 (10), 2665-2674 (2003)
   PUBMED   12750295
  REMARK    GeneRIF: EWI2/PGRL associates with the metastasis suppressor
            KAI1/CD82 and inhibits the migration of prostate cancer cells.
REFERENCE   9  (residues 1 to 613)
  AUTHORS   Stipp,C.S., Kolesnikova,T.V. and Hemler,M.E.
  TITLE     EWI-2 is a major CD9 and CD81 partner and member of a novel Ig
            protein subfamily
  JOURNAL   J Biol Chem 276 (44), 40545-40554 (2001)
   PUBMED   11504738
REFERENCE   10 (residues 1 to 613)
  AUTHORS   Clark,K.L., Zeng,Z., Langford,A.L., Bowen,S.M. and Todd,S.C.
  TITLE     PGRL is a major CD81-associated protein on lymphocytes and
            distinguishes a new family of cell surface proteins
  JOURNAL   J Immunol 167 (9), 5115-5121 (2001)
   PUBMED   11673522
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC053881.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: This gene encodes a member the EWI subfamily of the
            immunoglobulin protein superfamily. Members of this family contain
            a single transmembrane domain, an EWI (Glu-Trp-Ile)-motif and a
            variable number of immunoglobulin domains. This protein interacts
            with the tetraspanins CD81 and CD9 and may regulate their role in
            certain cellular functions including cell migration and viral
            infection. The encoded protein may also function as a tumor
            suppressor by inhibiting the proliferation of certain cancers.
            Alternate splicing results in multiple transcript variants that
            encode the same protein. [provided by RefSeq, Sep 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2 and 3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053881.1, AF407274.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000314485.12/ ENSP00000316664.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q23.2"
     Protein         1..613
                     /product="immunoglobulin superfamily member 8 precursor"
                     /note="CD81 partner 3; glu-Trp-Ile EWI motif-containing
                     protein 2; keratinocytes-associated transmembrane protein
                     4; prostaglandin regulatory-like protein"
                     /calculated_mol_wt=62174
     sig_peptide     1..27
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2879
     mat_peptide     28..613
                     /product="Immunoglobulin superfamily member 8.
                     /id=PRO_0000226247"
                     /note="propagated from UniProtKB/Swiss-Prot (Q969P0.1)"
                     /calculated_mol_wt=62174
     Region          28..149
                     /region_name="V-set"
                     /note="Immunoglobulin V-set domain; pfam07686"
                     /db_xref="CDD:462230"
     Site            50
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q969P0.1)"
     Region          155..174
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q969P0.1)"
     Region          <158..600
                     /region_name="PksD"
                     /note="Acyl transferase domain in polyketide synthase
                     (PKS) enzymes [Secondary metabolites biosynthesis,
                     transport and catabolism]; COG3321"
                     /db_xref="CDD:442550"
     Region          274..276
                     /region_name="EWI motif"
                     /note="propagated from UniProtKB/Swiss-Prot (Q969P0.1)"
     Region          318..419
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          322..326
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409355"
     Site            327
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:19159218; propagated from
                     UniProtKB/Swiss-Prot (Q969P0.1)"
     Region          342..345
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409355"
     Region          389..393
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409355"
     Region          403..408
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409355"
     Region          414..417
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409355"
     Region          451..545
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          458..462
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409355"
     Site            463
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:19159218; propagated from
                     UniProtKB/Swiss-Prot (Q969P0.1)"
     Region          472..480
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409355"
     Site            518
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:24275569; propagated from
                     UniProtKB/Swiss-Prot (Q969P0.1)"
     Region          527..531
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409355"
     Region          541..545
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409355"
     Site            580..600
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q969P0.1)"
     CDS             1..613
                     /gene="IGSF8"
                     /gene_synonym="CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1;
                     PGRL"
                     /coded_by="NM_052868.6:148..1989"
                     /db_xref="CCDS:CCDS1195.1"
                     /db_xref="GeneID:93185"
                     /db_xref="HGNC:HGNC:17813"
                     /db_xref="MIM:606644"
ORIGIN      
        1 mgalrptllp pslplllllm lgmgcwarev lvpegplyrv agtavsiscn vtgyegpaqq
       61 nfewflyrpe apdtalgivs tkdtqfsyav fksrvvagev qvqrlqgdav vlkiarlqaq
      121 dagiyechtp stdtrylgsy sgkvelrvlp dvlqvsaapp gprgrqapts pprmtvhegq
      181 elalgclart stqkhthlav sfgrsvpeap vgrstlqevv girsdlavea gapyaerlaa
      241 gelrlgkegt dryrmvvgga qagdagtyhc taaewiqdpd gswaqiaekr avlahvdvqt
      301 lssqlavtvg pgerrigpge plellcnvsg alppagrhaa ysvgwemapa gapgpgrlva
      361 qldtegvgsl gpgyegrhia mekvasrtyr lrleaarpgd agtyrclaka yvrgsgtrlr
      421 eaasarsrpl pvhvreegvv leavawlagg tvyrgetasl lcnisvrggp pglrlaasww
      481 verpedgels svpaqlvggv gqdgvaelgv rpgggpvsve lvgprshrlr lhslgpedeg
      541 vyhcapsawv qhadyswyqa gsarsgpvtv ypymhaldtl fvpllvgtgv alvtgatvlg
      601 titccfmkrl rkr
//
