LOCUS       NM_001170962            4434 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens immunoglobulin superfamily member 1 (IGSF1),
            transcript variant 4, mRNA.
ACCESSION   NM_001170962
VERSION     NM_001170962.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Zhang,C., Chen,L., Chen,X., Xie,R., Wang,F., Chen,T., Wang,X.,
            Sun,H., Zhang,D. and Wu,H.
  TITLE     [Clinical characteristics and genetic analysis of four patients
            with central hypothyroidism due to IGSF1 gene variants]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 40 (3), 322-327 (2023)
   PUBMED   36854408
  REMARK    GeneRIF: [Clinical characteristics and genetic analysis of four
            patients with central hypothyroidism due to IGSF1 gene variants].
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Costas Eimil,J. and Sanchez-Sobrino,P.
  TITLE     IGSF1 mutation as a cause of isolated central hypothyroidism
  JOURNAL   Endocrinol Diabetes Nutr (Engl Ed) 69 (10), 913-914 (2022)
   PUBMED   36464600
  REMARK    GeneRIF: IGSF1 mutation as a cause of isolated central
            hypothyroidism.
REFERENCE   3  (bases 1 to 4434)
  AUTHORS   Fourneaux,R., Reynaud,R., Mougel,G., Castets,S., Bretones,P.,
            Dauriat,B., Edouard,T., Raverot,G., Barlier,A., Brue,T.,
            Castinetti,F. and Saveanu,A.
  TITLE     IGSF1 mutations are the most frequent genetic aetiology of
            thyrotropin deficiency
  JOURNAL   Eur J Endocrinol 187 (6), 787-795 (2022)
   PUBMED   36201163
  REMARK    GeneRIF: IGSF1 mutations are the most frequent genetic aetiology of
            thyrotropin deficiency.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4434)
  AUTHORS   Elizabeth,M.S.M., Hokken-Koelega,A., Visser,J.A., Joustra,S.D. and
            de Graaff,L.C.G.
  TITLE     Case Report: A Detailed Phenotypic Description of Patients and
            Relatives with Combined Central Hypothyroidism and Growth Hormone
            Deficiency Carrying IGSF1 Mutations
  JOURNAL   Genes (Basel) 13 (4), 623 (2022)
   PUBMED   35456429
  REMARK    GeneRIF: Case Report: A Detailed Phenotypic Description of Patients
            and Relatives with Combined Central Hypothyroidism and Growth
            Hormone Deficiency Carrying IGSF1 Mutations.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4434)
  AUTHORS   Fourneaux,R., Castets,S., Godefroy,A., Grelet,M., Abeillon-du
            Payrat,J., Saveanu,A., Castinetti,F. and Reynaud,R.
  TITLE     Congenital Central Hypothyroidism Caused by a Novel IGSF1 Variant
            Identified in a French Family
  JOURNAL   Horm Res Paediatr 95 (3), 296-303 (2022)
   PUBMED   35350016
  REMARK    GeneRIF: Congenital Central Hypothyroidism Caused by a Novel IGSF1
            Variant Identified in a French Family.
REFERENCE   6  (bases 1 to 4434)
  AUTHORS   Tanaka,S., Tatsumi,K., Okubo,K., Itoh,K., Kawamoto,S., Matsubara,K.
            and Amino,N.
  TITLE     Expression profile of active genes in the human pituitary gland
  JOURNAL   J Mol Endocrinol 28 (1), 33-44 (2002)
   PUBMED   11854097
REFERENCE   7  (bases 1 to 4434)
  AUTHORS   Gui,Y. and Murphy,L.J.
  TITLE     Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) binds
            to fibronectin (FN): demonstration of IGF-I/IGFBP-3/fn ternary
            complexes in human plasma
  JOURNAL   J Clin Endocrinol Metab 86 (5), 2104-2110 (2001)
   PUBMED   11344214
REFERENCE   8  (bases 1 to 4434)
  AUTHORS   Chapman,S.C. and Woodruff,T.K.
  TITLE     Modulation of activin signal transduction by inhibin B and
            inhibin-binding protein (INhBP)
  JOURNAL   Mol Endocrinol 15 (4), 668-679 (2001)
   PUBMED   11266516
REFERENCE   9  (bases 1 to 4434)
  AUTHORS   Frattini,A., Faranda,S., Redolfi,E., Allavena,P. and Vezzoni,P.
  TITLE     Identification and genomic organization of a gene coding for a new
            member of the cell adhesion molecule family mapping to Xq25
  JOURNAL   Gene 214 (1-2), 1-6 (1998)
   PUBMED   9729118
REFERENCE   10 (bases 1 to 4434)
  AUTHORS   Mazzarella,R., Pengue,G., Jones,J., Jones,C. and Schlessinger,D.
  TITLE     Cloning and expression of an immunoglobulin superfamily gene
            (IGSF1) in Xq25
  JOURNAL   Genomics 48 (2), 157-162 (1998)
   PUBMED   9521868
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL590806.6.
            
            On Jun 1, 2019 this sequence version replaced NM_001170962.1.
            
            Summary: This gene encodes a member of the immunoglobulin-like
            domain-containing superfamily. Proteins in this superfamily contain
            varying numbers of immunoglobulin-like domains and are thought to
            participate in the regulation of interactions between cells.
            Multiple transcript variants encoding different isoforms have been
            found for this gene.[provided by RefSeq, Jan 2010].
            
            Transcript Variant: This variant (4) lacks an alternate in-frame
            exon in the 5' coding region and uses an alternate in-frame splice
            site in a 3' exon compared to variant 3. The resulting protein
            (isoform 4) is shorter compared to isoform 3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF034198.1, SRR14372079.2001561.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-93                AL590806.6         46379-46471         c
            94-230              AL590806.6         43770-43906         c
            231-512             AL590806.6         42932-43213         c
            513-800             AL590806.6         42344-42631         c
            801-1085            AL590806.6         40145-40429         c
            1086-1379           AL590806.6         39609-39902         c
            1380-1658           AL590806.6         38831-39109         c
            1659-1779           AL590806.6         38383-38503         c
            1780-1850           AL590806.6         36436-36506         c
            1851-1883           AL590806.6         36308-36340         c
            1884-2174           AL590806.6         35626-35916         c
            2175-2453           AL590806.6         35021-35299         c
            2454-2741           AL590806.6         34104-34391         c
            2742-3029           AL590806.6         33126-33413         c
            3030-3317           AL590806.6         32643-32930         c
            3318-3605           AL590806.6         32164-32451         c
            3606-3884           AL590806.6         31764-32042         c
            3885-4008           AL590806.6         31248-31371         c
            4009-4434           AL590806.6         30671-31096         c
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq26.1"
     gene            1..4434
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /note="immunoglobulin superfamily member 1"
                     /db_xref="GeneID:3547"
                     /db_xref="HGNC:HGNC:5948"
                     /db_xref="MIM:300137"
     exon            1..93
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            94..230
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..4144
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /note="isoform 4 precursor is encoded by transcript
                     variant 4; inhibin-binding protein; pituitary
                     gland-specific factor 2; immunoglobulin-like
                     domain-containing protein 1"
                     /codon_start=1
                     /product="immunoglobulin superfamily member 1 isoform 4
                     precursor"
                     /protein_id="NP_001164433.1"
                     /db_xref="CCDS:CCDS55490.1"
                     /db_xref="GeneID:3547"
                     /db_xref="HGNC:HGNC:5948"
                     /db_xref="MIM:300137"
                     /translation="MTLDRPGEGATMLKTFTVLLFCILMDPQPELWIESNYPQAPWEN
                     ITLWCRSPSRISSKFLLLKDKTQMTWIRPSHKTFQVSFLIGALTESNAGLYRCCYWKE
                     TGWSKPSKVLELEAPGQLPKPIFWIQAETPALPGCNVNILCHGWLQDLVFMLFKEGYA
                     EPVDYQVPTGTMAIFSIDNLTPEDEGVYICRTHIQMLPTLWSEPSNPLKLVVAGLYPK
                     PTLTAHPGPIMAPGESLNLRCQGPIYGMTFALMRVEDLEKSFYHKKTIKNEANFFFQS
                     LKIQDTGHYLCFYYDASYRGSLLSDVLKIWVTDTFPKTWLLARPSAVVQMGQNVSLRC
                     RGPVDGVGLALYKKGEDKPLQFLDATSIDDNTSFFLNNVTYSDTGIYSCHYLLTWKTS
                     IRMPSHNTVELMVVDKPPKPSLSAWPSTVFKLGKAITLQCRVSHPVLEFSLEWEERET
                     FQKFSVNGDFIISNVDGKGTGTYSCSYRVETHPNIWSHRSEPLKLMGPAGYLTWNYVL
                     NEAIRLSLIMQLVALLLVVLWIRWKCRRLRIREAWLLGTAQGVTMLFIVTALLCCGLC
                     NGVLIEETEIVMPTPKPELWAETNFPLAPWKNLTLWCRSPSGSTKEFVLLKDGTGWIA
                     TRPASEQVRAAFPLGALTQSHTGSYHCHSWEEMAVSEPSEALELVGTDILPKPVISAS
                     PTIRGQELQLRCKGWLAGMGFALYKEGEQEPVQQLGAVGREAFFTIQRMEDKDEGNYS
                     CRTHTEKRPFKWSEPSEPLELVIKEMYPKPFFKTWASPVVTPGARVTFNCSTPHQHMS
                     FILYKDGSEIASSDRSWASPGASAAHFLIISVGIGDGGNYSCRYYDFSIWSEPSDPVE
                     LVVTEFYPKPTLLAQPGPVVFPGKSVILRCQGTFQGMRFALLQEGAHVPLQFRSVSGN
                     SADFLLHTVGAEDSGNYSCIYYETTMSNRGSYLSMPLMIWVTDTFPKPWLFAEPSSVV
                     PMGQNVTLWCRGPVHGVGYILHKEGEATSMQLWGSTSNDGAFPITNISGTSMGRYSCC
                     YHPDWTSSIKIQPSNTLELLVTGLLPKPSLLAQPGPMVAPGENMTLQCQGELPDSTFV
                     LLKEGAQEPLEQQRPSGYRADFWMPAVRGEDSGIYSCVYYLDSTPFAASNHSDSLEIW
                     VTDKPPKPSLSAWPSTMFKLGKDITLQCRGPLPGVEFVLEHDGEEAPQQFSEDGDFVI
                     NNVEGKGIGNYSCSYRLQAYPDIWSEPSDPLELVGAAGPVAQECTVGNIVRSSLIVVV
                     VVALGVVLAIEWKKWPRLRTRGSETDGRDQTIALEECNQEGEPGTPANSPSSTSQRIS
                     VELPVPI"
     exon            231..512
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            513..800
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            801..1085
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1379
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1658
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1779
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1850
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..1883
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..2174
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..2453
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2454..2741
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2742..3029
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3030..3317
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3318..3605
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3606..3884
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3885..4008
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     exon            4009..4434
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4401..4406
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /note="hexamer: AATAAA"
     polyA_site      4434
                     /gene="IGSF1"
                     /gene_synonym="CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2"
                     /note="major polyA site"
ORIGIN      
        1 gtccttagac agctcttctc atgccgggca gtttgctgca tctggaggag ctcactggag
       61 aatctccaac atcggagcgg gccttcaact accatcccac cacctgctga ggagaaaaat
      121 tcttcaagac tcagagcaca cagccagcac cagaggcccc atgaccctgg acagaccagg
      181 ggagggggcc accatgctga agacattcac tgttttgctc ttttgcattc tgatggaccc
      241 tcaaccagag ttgtggatag agtccaacta cccccaggcc ccttgggaga acatcacgct
      301 ttggtgccga agcccctctc ggatatcaag caagttcctg ctgctgaagg ataagacaca
      361 gatgacctgg atccgccctt cccacaagac cttccaagtt tcattcctta taggtgccct
      421 tactgagtcc aatgcaggtc tttaccggtg ctgctactgg aaggagacag gctggtcaaa
      481 gcccagtaaa gttctagagt tggaggcacc aggccaactg cccaagccca tcttctggat
      541 tcaggctgag acccccgctc ttcctgggtg taatgttaac atcctctgcc atggctggct
      601 gcaggatttg gtattcatgc tgtttaaaga gggatatgca gagcctgtgg attaccaagt
      661 cccaactggg acaatggcca tattctccat tgacaacctg acacctgagg atgaaggggt
      721 ttacatctgc cgcactcata tccagatgct ccccaccctg tggtcagagc ccagcaaccc
      781 cctgaagctg gttgtagcag gactctaccc caaaccaact ttgacagccc atcctgggcc
      841 catcatggca cctggagaaa gcctgaatct caggtgccaa gggccaatct atggaatgac
      901 ctttgctcta atgagggttg aagacttgga gaagtccttt taccacaaga agacaataaa
      961 aaatgaggca aatttcttct tccagtcttt gaagatccaa gatactggac attacctctg
     1021 tttttactat gacgcatcat atagaggttc actccttagt gatgtcctga aaatctgggt
     1081 gactgacact ttccccaaga cctggctact tgctcggccc agtgctgtgg tccaaatggg
     1141 tcagaatgtg agcctacggt gtcgaggacc agtggatgga gtgggtcttg cactctataa
     1201 gaaaggagaa gacaaaccac ttcaattttt ggatgccacc agcatcgatg acaacacatc
     1261 attcttcctc aacaatgtaa cctacagtga tactggcatc tatagctgcc actatcttct
     1321 cacctggaag acctccatta ggatgccatc acacaacact gtggagctta tggttgtaga
     1381 taagcccccc aaaccctccc tgtcagcttg gccaagcact gtgttcaagc taggaaaggc
     1441 catcaccctt cagtgccgag tatctcatcc agtactggaa ttttctctgg aatgggaaga
     1501 aagagaaaca ttccaaaaat tctcagtaaa cggagacttc atcatcagta atgttgacgg
     1561 gaaaggcaca gggacctaca gttgcagcta tcgcgtagag acacatccta acatctggtc
     1621 acatcgcagt gagcccctga agctgatggg gccagcaggc tatctcacct ggaattacgt
     1681 tctgaatgaa gctatcaggt tgtctctaat catgcagctt gttgccttgc tgttggtagt
     1741 gctgtggata aggtggaagt gtcggagact cagaatcaga gaagcctggt tgctgggaac
     1801 agctcaaggg gtcaccatgc tcttcatagt cacggccctt ctctgctgtg gactgtgcaa
     1861 tggggtattg atagaagaga ctgaaatagt catgccaacc cctaagcctg agctgtgggc
     1921 agagaccaac tttcctctgg ccccgtggaa gaacttaacc ctctggtgca gaagcccttc
     1981 tggctcaact aaggagtttg tgttgctgaa ggatgggacc gggtggatcg ccactcgccc
     2041 ggcctcagag caggtccggg ctgccttccc ccttggcgcc ctgacccaga gccacaccgg
     2101 gagctaccac tgccattcat gggaggagat ggctgtatcg gagcccagtg aggcacttga
     2161 gctggtgggg acagacatcc tccccaaacc tgtcatttct gcttccccca caatccgggg
     2221 ccaggaacta caactccggt gcaaaggatg gctggcaggc atggggtttg ctctgtataa
     2281 ggagggagag caagaacctg tccagcaact tggtgctgtt ggaagagaag ccttctttac
     2341 aatccagaga atggaggata aagacgaagg caattacagc tgccgcactc acactgaaaa
     2401 acgccccttc aagtggtctg agcccagtga gccgctggag cttgtcataa aagaaatgta
     2461 ccctaagccc ttcttcaaga catgggccag ccctgtggtc acccctggtg cccgagtgac
     2521 tttcaattgc tccacccccc accagcatat gagctttatt ctttacaaag atggaagtga
     2581 aatagcatcc agtgacaggt cctgggcaag tccgggggcc agtgcagctc actttctaat
     2641 catttcggtg ggcattggtg atggagggaa ttacagctgc cgatattatg acttttctat
     2701 ctggtctgag cccagcgacc ctgtggagct cgtggtgaca gaattctacc ccaaacccac
     2761 tctcctggca cagccaggtc ctgtggtgtt tcctgggaag agtgtgatcc tgcgctgcca
     2821 agggactttc cagggcatga ggttcgccct cttgcaggag ggagcccatg ttcccttaca
     2881 gtttcggagt gtctcaggga actcagctga cttccttctc cacactgttg gagcagagga
     2941 ctctgggaac tatagctgta tctactatga gacaaccatg tcaaacaggg ggtcatatct
     3001 cagtatgccc cttatgatct gggtgactga cacattccct aagccatggt tgtttgctga
     3061 gcccagttct gtggttccca tggggcagaa tgttactctc tggtgccgag ggccggtcca
     3121 tggagtagga tacattctgc acaaagaagg agaagccact tcaatgcagc tctggggatc
     3181 caccagtaat gacggggcat tccccatcac caatatatct ggtactagca tggggcgtta
     3241 cagctgctgc taccaccctg actggaccag ttctatcaag atacaaccta gcaacaccct
     3301 ggaactccta gtcacaggct tactccccaa acccagccta ttagcccagc ctggtcccat
     3361 ggtggcccct ggcgaaaata tgactcttca gtgtcaaggg gaactgccag actcaacatt
     3421 tgtcctgttg aaggaggggg ctcaggagcc tttagagcaa cagaggccaa gtgggtacag
     3481 ggctgacttc tggatgccag cagtgagagg tgaagactct gggatctata gctgtgttta
     3541 ttatttggac tctactccct ttgcagcttc aaatcacagt gactccctgg agatctgggt
     3601 gactgataag ccccctaaac cctctctgtc agcctggccc agcaccatgt tcaagttagg
     3661 gaaggacatc acccttcagt gccgaggacc cctgccaggt gttgaatttg tcctagaaca
     3721 tgatggagaa gaagcacctc agcagttttc agaggatgga gactttgtca tcaacaacgt
     3781 agaaggaaaa ggcattggaa actacagctg cagctaccgc ctccaggcct accctgatat
     3841 ctggtcagag cctagtgatc ccctggagct ggtgggggca gcagggcctg ttgctcagga
     3901 gtgcactgta gggaacattg tccgaagtag cctaatcgtg gtggttgttg tagccttggg
     3961 ggtagtgcta gccatagagt ggaagaagtg gcctcgactg cgaaccagag gctcagagac
     4021 agacggaaga gaccagacca ttgcccttga agagtgtaac caagaaggag aaccaggcac
     4081 ccctgccaat tctccttcat caacctctca gagaatctct gtggaactgc ccgttccaat
     4141 ataataatct cctcctttac aagagctttc ctctcctctc tcttgctctc agagacctat
     4201 aaatccaacc agttaccctg caagtcagcc ccatctgctg ttccttggtc tctaatcacc
     4261 tgagctgggt aaaggggatt ctgggagttg agagctctgc cagggtgaga tgtttcctga
     4321 agagaggttc cccacccctg taactcctca ctgtactgat ttactggcgc atgaaattct
     4381 attaaaaatg cattcttctg aataaaaaga gtattcacta tttaacttca aaaa
//
