LOCUS       NM_001164586           11811 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens immunoglobulin like and fibronectin type III domain
            containing 1 (IGFN1), transcript variant 1, mRNA.
ACCESSION   NM_001164586
VERSION     NM_001164586.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 11811)
  AUTHORS   Verma,S.P. and Das,P.
  TITLE     Novel splicing in IGFN1 intron 15 and role of stable G-quadruplex
            in the regulation of splicing in renal cell carcinoma
  JOURNAL   PLoS One 13 (10), e0205660 (2018)
   PUBMED   30335789
  REMARK    GeneRIF: Study results in UOK146 renal cell carcinoma cell line
            provide evidence of novel splicing in intron 15 of IGFN1 leading to
            the formation of two more alternative spliced isoforms. Potential
            G-quadruplex forming sequence (PQS) in this intron forms a stable
            G-quadruplex which is involved in the alternative splicing and
            regulates splicing efficiency.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11811)
  AUTHORS   Wen,X., Liu,Y., Yan,Q., Liang,M., Tang,M., Liu,R., Pan,J., Liu,Q.,
            Chen,T., Guo,S., Liang,J., Lu,L., Ding,X., Chen,W. and Wei,L.
  TITLE     Association of IGFN1 variant with polypoidal choroidal vasculopathy
  JOURNAL   J Gene Med 20 (2-3), e3007 (2018)
   PUBMED   29323771
  REMARK    GeneRIF: A novel variant, c.6196A>G in the IGFN1 gene, was
            significantly associated with only Polypoidal choroidal
            vasculopathy (combined p = 7.1 x 10(-11) , odds ratio = 9.44), but
            not with neovascular age-related macular degeneration(combined p =
            0.683, odds ratio = 1.30).
REFERENCE   4  (bases 1 to 11811)
  AUTHORS   Mansilla,F., Dominguez,C.A., Yeadon,J.E., Corydon,T.J., Burden,S.J.
            and Knudsen,C.R.
  TITLE     Translation elongation factor eEF1A binds to a novel myosin binding
            protein-C-like protein
  JOURNAL   J Cell Biochem 105 (3), 847-858 (2008)
   PUBMED   18756455
  REMARK    GeneRIF: a model in which this increased expression of IGFN1 serves
            to down-regulate protein synthesis via interaction with eEF1A
            during denervation.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC103925.2.
            
            On Aug 5, 2019 this sequence version replaced NM_001164586.1.
            
            Sequence Note: The structure of the RefSeq transcript and protein
            are inferred from partial human and mouse transcripts, conservation
            of predicted coding sequence between human, mouse, and rat, and
            Northern analyses by Mansilla et al (2008, PMID: 18756455)
            detecting a single 10+ kb transcript in human skeletal muscle
            tissue.
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: PMID: 18756455
            MANE Ensembl match        :: ENST00000335211.9/ ENSP00000334714.4
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-84                AC103925.2         156713-156796       c
            85-138              AC103925.2         154320-154373       c
            139-258             AC103925.2         153347-153466       c
            259-398             AC103925.2         151642-151781       c
            399-498             AC103925.2         150303-150402       c
            499-543             AC103925.2         148242-148286       c
            544-589             AC103925.2         147966-148011       c
            590-764             AC103925.2         147209-147383       c
            765-878             AC103925.2         145788-145901       c
            879-1047            AC103925.2         143714-143882       c
            1048-1320           AC103925.2         142266-142538       c
            1321-8859           AC103925.2         133999-141537       c
            8860-8984           AC103925.2         133319-133443       c
            8985-9126           AC103925.2         132466-132607       c
            9127-9426           AC103925.2         131782-132081       c
            9427-9726           AC103925.2         130867-131166       c
            9727-9900           AC103925.2         130160-130333       c
            9901-10029          AC103925.2         128962-129090       c
            10030-10332         AC103925.2         125874-126176       c
            10333-10421         AC103925.2         124793-124881       c
            10422-10617         AC103925.2         122746-122941       c
            10618-10917         AC103925.2         121497-121796       c
            10918-11244         AC103925.2         120412-120738       c
            11245-11811         AC103925.2         118668-119234       c
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..11811
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /note="immunoglobulin like and fibronectin type III domain
                     containing 1"
                     /db_xref="GeneID:91156"
                     /db_xref="HGNC:HGNC:24607"
                     /db_xref="MIM:617309"
     exon            1..84
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            85..138
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..11258
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     immunoglobulin-like and fibronectin type III
                     domain-containing protein 1; eEF1A2 binding protein;
                     EEF1A2-binding protein 1; KY-interacting protein 1"
                     /codon_start=1
                     /product="immunoglobulin-like and fibronectin type III
                     domain-containing protein 1 isoform 1"
                     /protein_id="NP_001158058.1"
                     /db_xref="CCDS:CCDS53455.1"
                     /db_xref="GeneID:91156"
                     /db_xref="HGNC:HGNC:24607"
                     /db_xref="MIM:617309"
                     /translation="MAGKLRKSHIPGVSIWQLVEEIPEGCSTPDFEQKPVTSALPEGK
                     NAVFRAVVCGEPRPEVRWQNSKGDLSDSSKYKISSSPGSKEHVLQINKLTGEDTDLYR
                     CTAVNAYGEAACSVRLTVIEVGFRKNRKRHREPQEDLRKELMDFRKLLKKRAPPAPKK
                     KMDLEQIWQLLMTADRKDYEKICLKYGIVDYRGMLRRLQEMKKEQEDKMAQYINTISS
                     LRHIRVTKDGNAKFDLELDLKDSQSKIYLYKDGEMIPYGFNNQTKHCLRRLGKRYEFQ
                     IQDLRPEDSGIYQVKVEDAVVFSTELEASAIPPRVVVPLAETHCEEQGDAVFECTLSS
                     PCPSAAWHFRHRLLHPSDKYEVYVSPDGLTHRLVVRGARFSDMGPYSLGTGLYTSSAW
                     LVVEAGKDKDLQSTSADHKLQRQGAQASGAEESGSIESQGEKSREQGPRGGSLEGAGP
                     ASGLQHIASPDRDGLGRHGYSLMGDKGTADSAWGPGQEGEGFPVAEGSRATLPRENQS
                     HREGGWARSLAERPHLQGESSESGLGLPEKQQQDRGRDSNSDECWRKAGGWEAGSSRL
                     QAGGLGSSREGKEHRGDSGRQLDRHAPEQLWDARLGPGRGKSDLQGCQSDPVGSWPRG
                     KQIEISQDDSLAEMDRGDAPSRERGRGIVVWGGGTGLGEAGDSNGAGGPGTLELTGGR
                     GSGSKVGMAPESWGSQGGRDADYGEARGYWGSGELLEQIPGGKDFQEPSISGGRKFLL
                     GDGSPEIKAEDSLQEADGICRGESVVTGSAYKTGPGGPGDPRGCEGVLQELRGRDGQE
                     TAWASGEVEYDPRSFQSSQGWTAGHRAAGGIGRIESKGTSPWDDTPSSLRKTGAHHGP
                     GVLGPSGGQEGMGGIWVAGLTESGQGVDARSHWLSRAPGLGAQGSGGTLGDKKGLRGP
                     GSIGSEPDFWNGSGSSRVKGPRGETGYKDGLEGPGRMESRYEGGLGYSREISSKSGAG
                     YSYGSGVPGEMGSGHGAGCRVSPRAPAGVESEEGGGYRHGSGAPGGVWSGNEDSGPAG
                     GGSGRVASLKNGSGGPDGAPMNDTRNWASACQAGMDPRGGHHSDGGLGSPGVTGSAGR
                     GGLKAPGVVETVGMGCVEAEPESSGRIRPWGQTGNYGGFRASEALGAFGEGGYEDGSG
                     GPGAMGPGSLRAGSKVGEGDGTRCPGAKASGAGAGYRDDTRHPESLAPHNGAASGSQW
                     AYGAGNVLGYEDGSELPGPQGTGVRTAYGERSRGLGPRSTGPGGEAGFRDGSGGLQGM
                     GSADGPGCRKGIGSSGEMGSVDKEGYKKDLGAPENMGSGSKADYRDGVGGSGAMGSMD
                     EAGYRKDLGAPEGISSGSKADYRGGLQDSREAGSGSKADYSGGLKGSREIGSMDETDN
                     RKDLGVPEGMGAGYRAGLRGPGEMGSLDESGHRNGIGGYGEMGSGYREDLGAPEGMGT
                     GSKAGYRDGLRGSGEMRSMDEAGYRKNLGAPERMDSGSKAGYRGGLRGSGEMGLIEAG
                     YRKDLGVSEGGGSGSKAGYRGGLGSGEMGSVDKAGYRKDLGASEAIGSGSKAGFTDGL
                     GGSEEMGSVNKAGYRKDLGAPKGMGSGSKASFRDGLGGSGEMGSVNEAGYRKDLGVPE
                     GIGSGSKAGFRDGLGGSEEMGSVNKAGYRKDLGAPKGIGSGSKAGFRDGLGSSGEMGS
                     MDEAGYRKNLGAPEGIGSGSKAGFRDGLGSSVEMGSVNEAGYRKDLGAPEGMGSGSKA
                     GFRDGLGGSGEMGSVNEAGYRKDLGAPKGIGSGSKADFRDALGSSGEMGSMDEAGYRK
                     DLWAPEGIGSGSKAGFRDGLGSSVEMGSVNEAGYRKDLGAPEGMGSGSKAGFRDGLGG
                     SGEMGSVNEAGYRKDLGAPKGIGSGSKADFRDALGSSGEMGSMDEAGYRKDLWAPEGI
                     GSGSKAGFRDGLGSSVEMGSVNEAGYRKDLGAPEGMGSGSKEGFRDGLGGSEEMGSVN
                     KAGYRKDLGAPKGMGSGSKEGFRDGLGGSEEMGSMDEAGYRKDLGAPEGIGSGSKAGF
                     RDGLGGSEEMRSMDEAGYRKDLGAPERIGSGSKAGFRDGLGSSVEMGSVNEAGYRKDL
                     GAPKGMGSGSKTGFRDGLGGSEEMESMDEAGYRKDLGAPEGIGSGSKAGFRDGLGSST
                     EMGSVNEAGYRKDLGAPKGMGSESKAGFRDGLGSSGEMGSMDEAGYRKDLGAPEGMGS
                     GSKAGFRDGLGGSEEMGSVNKAGYRKDLGAPKGMGSGSKAGFRDGLGSSGEMGSMDEA
                     DYRKDLGAPEEMGSGSYTDYRNGLGSSGKISSGDEAGYKNVLGGSGRNPLGSEAGSRG
                     SLEDSGYILSWNEAGSRQGFGGTSGMGSGSEVSYRGGSGGSGETGPEGKMGYGDGSGR
                     LGVPGSLAGIGHEAGPRGHKAMGHRSGYWVASEGDTNSKDGPERARETRLVDGAGPGV
                     EPGMAGMPGTAGGMAHRDSLRGTGVLGSQGGRQTLSDERGSTKDLGGYGTSGIPEASE
                     AAGAKGKPDVKEWQDSSGTPGSSRDRGAPRVKDRSPDQAGIMGASGFLDGKGAVEGET
                     WAGMAALGSGYERDIWKAGPGMTDRGRVAGQGGLASQGGGDSLLGGRRVGSGSSVGTG
                     QDLDSGSMPGGRGKSTSGPADRQGTSNAWAPDWENQGFSQGSIDAGKQPAGSRASGSL
                     QEKDAAFGGTHEGPGGFKGGEGAPGQEAAGGCRSPWSLDSKGSSPGRGSSVDAEDSGI
                     LGKGNSTEWGNALTPKPGESGPQGAWNGLDGPFGRKASRDRSGGTQDLSSQRGKGQRG
                     GKRSLGEQGSLEAENGEVQGPGALKEDEGQGVEEAGRSGRRPGSLRSRSQAQSGAEVG
                     GGKRRGADEAGSMGWQPMGENWGCLEEMLNEDQSREPPGHLGSRRSGKDGRLDIYGER
                     RDATRSSTSRYKPGTGSFSKDAQGPMGHFSQGLADMEVQPGEAATLSCTLTSDLGPGT
                     WFKDGVKLTTQDGVIFKQDGLVHSLFITHVQGTQAGRYTFVAGDQQSEATLTVQDSPT
                     IAPDVTEKLREPLVVKAGKPVIVKIPFQSHLPIQAAWRKDGAEVVGSSDREAQVDLGD
                     GYTRLCLPSAGRKDCGQYSVTLRSEGGSVQAELTLQVIDKPDPPQGPMEVQDCHRAGV
                     CLRWRPPRDNGGRTVECYVVERRQAGRSTWLKVGEAPADSTTFTDAHVEPGRKYTFRV
                     RAVTSEGAGEALESEEILVAPEALPKAPSAPAILSASSQGITLTWTAPRGPGSAHILG
                     YLIERRKKGSNTWTAVNDQPVPERRWTVADVRQGCQYEFRVTAVAPSGPGEPGPPSDA
                     VFARDPMRPPGLVRNLQVTDRSNTSITLSWAGPDTQEGDEAQGYVVELCSSDSLQWLP
                     CHVGTVPVTTYTAKGLRPGEGYFVRVTAVNEGGQSQPSALDTLVQAMPVTVCPKFLVD
                     SSTKDLLTVKVGDTVRVPVSFEAMPMPEVTWLKDGLPLPKRSVTVTKDGLTQLLIPVA
                     GLSDSGLYTVVLRTLQGKEVAHSFRIRVAACPQAPGPIHLQENVPGTVTAEWEPSPDE
                     AQDVPLHYAVFTRSSAHGPWHEAADRIHTNRFTLLGILPGHEYHFRVVAKNELGASKP
                     SDTSQPWCIPRQRDRFTVKAPCYREPDLSQKPRFLVGLRSHLLPQGCECCMSCAVQGS
                     PRPHVTWFKNDRSLEGNPAVYSTDLLGVCSLTIPSVSPKDSGEYKAVAENTLGQAVST
                     ATLIVIEPST"
     exon            139..258
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            259..398
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            399..498
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            499..543
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            544..589
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            590..764
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            765..878
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1047
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1320
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1321..8859
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            8860..8984
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            8985..9126
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            9127..9426
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            9427..9726
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            9727..9900
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            9901..10029
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            10030..10332
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            10333..10421
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            10422..10617
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            10618..10917
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            10918..11244
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     exon            11245..11811
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11786..11791
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /note="hexamer: ATTAAA"
     polyA_site      11811
                     /gene="IGFN1"
                     /gene_synonym="EEF1A2BP1"
                     /note="major polyA site"
ORIGIN      
        1 agtcaatcca gagcctcagc aagcagcagc tggaaacggg gaagagggag cagacaggaa
       61 gcgggaggtc agctgtacac ccagggtaat agaacttcta ccctcagagg agtcaaagag
      121 gaggcagaac tatggcagga aagctccgga agtcccacat ccctggagtg agcatctggc
      181 agctggtgga ggagatccct gaaggctgca gcacgccgga ctttgagcag aagcccgtca
      241 cctcggctct gccagagggg aaaaatgctg tctttcgggc tgtggtctgt ggggagccca
      301 ggcccgaggt gcgttggcag aactccaaag gtgacctcag tgattccagc aagtacaaga
      361 tctcctccag ccctggcagc aaggagcacg tgctgcagat caacaagctg acaggcgagg
      421 acacggatct gtaccgctgc acagcagtaa atgcgtacgg agaggccgct tgctcagtga
      481 gactcactgt catcgaagtt ggctttcgga agaatcggaa gaggcacagg gaaccgcagg
      541 aagacctcag gaaggagctg atggacttcc ggaagttgct gaaaaagagg gccccaccag
      601 cccccaagaa aaagatggac cttgagcaga tatggcagct gctgatgaca gcagacagga
      661 aggactacga gaagatctgc ttgaagtatg gcatcgtcga ctaccgtggc atgttgcgca
      721 ggctgcagga gatgaagaag gaacaggagg acaagatggc acagtacatc aacaccatct
      781 ccagcttaag acacatcagg gtcaccaagg atgggaatgc aaagtttgac ttggagctgg
      841 atctcaagga ttctcagagc aagatttacc tgtataagga tggtgagatg atcccctatg
      901 gcttcaacaa ccaaaccaag cactgtctgc gccggctggg gaagcgctat gagttccaga
      961 ttcaagacct gaggcctgag gactctggca tttaccaggt caaggtggag gatgctgtgg
     1021 tcttctccac agaactggag gccagtgcca tccccccaag agtggtggtc ccactggcgg
     1081 agacccactg tgaggagcag ggtgacgcag tctttgaatg taccctctcc agcccctgcc
     1141 ctagtgcagc ctggcatttc cggcaccggc tactccaccc cagtgacaaa tatgaagtgt
     1201 atgtgtcccc tgacgggctg acccaccggc tggtggtgag gggggcacgt ttctcagaca
     1261 tgggccccta ttcgctgggc accgggctct acacttccag cgcctggctg gtggttgaag
     1321 ctgggaagga taaagacctt cagtccacaa gtgctgacca caaactgcag aggcaaggag
     1381 cccaggcatc aggagcagaa gagtctggga gcatcgagag ccagggagag aaatccagag
     1441 agcagggccc cagggggggc tcccttgaag gggctgggcc ggcttctggg ctccagcaca
     1501 tagccagccc agacagggat ggccttggca gacatggcta ctccttgatg ggggacaaag
     1561 ggacagctga ctcagcctgg ggccctggac aggagggcga gggctttcca gtagcagagg
     1621 gaagcagagc cactcttccc agggaaaatc aatcccacag agagggaggc tgggccagaa
     1681 gccttgcaga gaggccccat ctacagggag agagctcaga atcagggttg ggcctcccag
     1741 aaaaacaaca gcaagatcgt ggcagagaca gcaacagtga tgaatgctgg aggaaagcag
     1801 gaggctggga ggctgggtcc agtcggcttc aggctggagg actggggagc agcagggaag
     1861 gaaaggagca cagaggggac agtggaagac aactggacag gcatgcccca gagcaactgt
     1921 gggatgctcg actgggacct gggagaggaa agagcgacct gcagggatgc cagtctgatc
     1981 ctgtagggtc ctggccaaga ggaaagcaga tagagatttc acaggatgac agcctggctg
     2041 agatggacag aggggatgct ccaagtaggg aaagggggag aggaatagta gtgtggggtg
     2101 gtgggactgg cctgggagaa gctggagaca gcaatggggc aggaggtcct ggcaccctgg
     2161 agcttactgg aggaagaggt tctggctcca aggtgggcat ggcccctgaa tcctggggtt
     2221 ctcagggagg tagagatgct gactatgggg aagccagggg ctactggggg tcaggagagt
     2281 tgctagaaca gatacctgga ggcaaggact tccaggaacc atcaatatca ggtggtagaa
     2341 aattccttct gggagatggg agtcctgaga tcaaagctga agactcactg caggaggcag
     2401 atggtatatg ccggggggag tctgtagtta caggaagtgc ctacaaaact ggccctggag
     2461 gcccaggaga ccccagaggc tgcgaaggtg tcctacagga gctcagggga agggatggcc
     2521 aggaaacagc ttgggcctcg ggtgaggtag agtatgaccc cagaagcttc cagtcttccc
     2581 agggctggac agcaggtcac agagcagcag ggggtattgg cagaatagaa tctaagggca
     2641 caagtccttg ggatgacaca ccatctagcc tcagaaaaac tggggcccac catgggcctg
     2701 gagtgctggg gcccagtgga ggacaagagg gtatgggtgg tatctgggtg gctggactga
     2761 cggagtctgg tcagggggtg gatgccagaa gccactggct aagtagggct ccaggcctgg
     2821 gtgctcaggg atctgggggg acactaggag ataagaaagg attaagaggt cctgggtcaa
     2881 tagggtctga accagatttc tggaatgggt cagggagctc cagagtaaaa ggacccagag
     2941 gtgagacagg ctataaggat ggcttggaag gtcccgggag aatggaatct aggtacgagg
     3001 gtggcttagg atattctagg gaaataagct ctaaaagcgg ggctggttat agctatggct
     3061 caggggttcc aggagaaatg gggtccggcc atggtgctgg ttgtagagtt tcccctaggg
     3121 cacctgcggg agtggagtct gaggaagggg gtgggtacag gcatggctcc ggagcgcctg
     3181 ggggagtgtg gtctggaaat gaagattctg gccctgcagg aggagggtct gggagagttg
     3241 ccagtcttaa aaatggctca ggtggtcctg atggagcacc catgaatgac accaggaatt
     3301 gggcctctgc atgccaggca ggcatggacc ctaggggagg gcaccattca gatggtggcc
     3361 tagggagtcc tggggtgaca gggtctgcgg gtagaggtgg tctcaaggcc cctggagtag
     3421 tggagactgt tgggatggga tgtgtggaag cagaaccaga gagctctgga agaataaggc
     3481 cttggggtca gactggaaat tatgggggct tcagagcctc agaggccctg ggggcctttg
     3541 gagaaggagg ctatgaagat ggctctgggg gtccaggagc catgggacca gggtctctga
     3601 gggcaggaag caaagtgggt gagggggatg ggacaagatg ccctggtgct aaggcctctg
     3661 gagctggagc tggttatagg gatgatacca ggcaccctga gtcactcgca cctcacaatg
     3721 gggccgcttc tgggagccag tgggcttatg gggctggcaa tgtgctgggt tatgaggatg
     3781 gatcagaact tccagggcct cagggaactg gggtcagaac agcctatgga gaaaggtcaa
     3841 ggggccttgg gcctaggagt acagggccag ggggtgaggc aggctttaga gatggttcag
     3901 gaggcctcca aggaatggga tcagcagatg ggccaggttg taggaagggt attgggagtt
     3961 ctggggaaat ggggtcagtg gataaggaag gttataagaa agatttgggg gctcctgaga
     4021 atatgggttc ggggagcaag gcagattata gggatggtgt agggggttct ggggcaatgg
     4081 ggtcaatgga tgaagcaggt tataggaaag atttaggggc tcctgaggga ataagttcag
     4141 ggagcaaggc agattatagg ggtggtttac aggattccag ggaagcgggt tcagggagca
     4201 aggcagatta tagcggtggt ttaaagggtt ccagggaaat cgggtcaatg gatgaaacag
     4261 ataataggaa agatttgggg gttcctgagg gaatgggtgc aggttacagg gctggtttaa
     4321 ggggtcctgg ggagatgggg tcactggatg agtcaggtca taggaatggg attggaggtt
     4381 atggggaaat ggggtcaggt tatagggagg atttgggggc tcctgaggga atgggcacag
     4441 ggagcaaggc aggttatagg gatggcttaa ggggttctgg agaaatgagg tcaatggatg
     4501 aggcaggtta taggaaaaat ttgggagctc ctgagagaat ggattcaggg agcaaggcag
     4561 gttacagggg tggtttaagg ggttctgggg aaatggggtt aattgaggca ggctatagga
     4621 aagatttggg ggtttctgag ggagggggtt cagggagcaa agcaggttat aggggtggct
     4681 taggttctgg ggaaatgggg tctgtggata aggcaggcta taggaaggat ttgggggctt
     4741 ctgaggcaat aggttcaggg agtaaggcag gttttacgga tggtttagga ggttctgaag
     4801 aaatggggtc agtgaataag gcaggttata ggaaggattt gggggctcct aagggaatgg
     4861 gttcagggag taaggcaagt tttagggatg gtttaggagg ttctggagaa atggggtcag
     4921 tgaatgaagc aggttatagg aaggacttgg gggttcctga gggaataggt tcaggaagta
     4981 aggcaggttt tagggatggt ttagggggtt ctgaagaaat ggggtcagtg aataaggcag
     5041 gttataggaa ggatttgggg gctcctaagg gaataggttc agggagcaag gcaggtttta
     5101 gggatggttt agggagttct ggggaaatgg ggtcaatgga tgaggcaggt tataggaaga
     5161 atttgggggc tcctgaggga ataggttcag ggagtaaggc aggttttagg gatggtttag
     5221 ggagttctgt agaaatgggg tcagtgaatg aggcaggtta taggaaggat ttgggggctc
     5281 ctgagggaat gggttcaggg agtaaggcag gtttcaggga tggtttaggg ggttctggag
     5341 aaatggggtc agtgaatgaa gcaggttata ggaaggattt gggtgctcct aagggaatag
     5401 gttcagggag caaggcagat tttagggatg ctttagggag ttctggggaa atggggtcaa
     5461 tggatgaggc aggttatagg aaggatttgt gggctcctga gggaataggt tcaggaagta
     5521 aggcaggttt tagggatggt ttagggagtt ctgtagaaat ggggtcagtg aatgaggcag
     5581 gttataggaa ggatttgggg gctcctgagg gaatgggttc agggagtaag gcaggtttca
     5641 gggatggttt agggggttct ggagaaatgg ggtcagtgaa tgaagcaggt tataggaagg
     5701 atttgggtgc tcctaaggga ataggttcag ggagcaaggc agattttagg gatgctttag
     5761 ggagttctgg ggaaatgggg tcaatggatg aggcaggtta taggaaggat ttgtgggctc
     5821 ctgagggaat aggttcagga agtaaggcag gttttaggga tggtttaggg agttctgtag
     5881 aaatggggtc agtgaatgag gcaggttata ggaaggattt gggggctcct gagggaatgg
     5941 gttcagggag taaggaaggt ttcagggatg gtttaggggg ttctgaagaa atggggtcag
     6001 tgaataaggc aggttatagg aaggatttgg gggctcctaa gggaatgggt tcagggagta
     6061 aggaaggttt cagggatggt ttagggggtt ctgaggaaat ggggtcaatg gatgaggcag
     6121 gttataggaa ggatttgggg gctcctgagg gaataggttc agggagtaag gcaggtttca
     6181 gggatggttt agggggttct gaagaaatgc ggtcaatgga tgaggcaggt tataggaagg
     6241 atttgggggc tcctgagaga ataggttcag gaagtaaggc aggttttagg gatggtttag
     6301 ggagttctgt agaaatgggg tcagtgaatg aggcaggtta taggaaggat ttaggggctc
     6361 ctaagggaat gggttcaggg agtaagacag gtttcaggga tggtttaggg ggttctgaag
     6421 aaatggagtc aatggatgag gcaggttata ggaaggattt gggggctcct gagggaatag
     6481 gttcaggaag taaggcaggt tttagggatg gtttagggag ttctacagaa atggggtcag
     6541 tgaatgaggc aggttatagg aaggatttgg gggctcctaa gggaatgggt tcagagagta
     6601 aggcaggttt tagggatggt ttagggagtt ctggggaaat ggggtcaatg gatgaggcag
     6661 gttataggaa ggatttggga gctcctgagg gaatgggttc agggagtaag gcaggtttca
     6721 gggatggttt agggggttct gaagaaatgg ggtcagtgaa taaggcaggt tataggaagg
     6781 atttgggggc tcctaaggga atgggttcag ggagtaaggc aggtttcagg gatggtttag
     6841 ggagttctgg ggaaatgggg tcaatggatg aggcagatta taggaaggat ttgggagctc
     6901 ctgaggaaat gggttcaggc agttacacag attacaggaa tggtttaggc agttctggaa
     6961 aaatcagttc aggggatgag gcaggttata agaatgtttt agggggttct gggaggaatc
     7021 cattagggag cgaggcaggt tctaggggta gtttggagga ttctgggtac attttgtcat
     7081 ggaatgaggc aggttctagg caaggctttg ggggaactag tggcatgggg tcagggagtg
     7141 aggtcagtta tagaggaggc tcaggaggat ctggggaaac gggaccagag ggtaagatgg
     7201 gttatggaga tggttcaggg aggcttggag taccaggctc actggctgga ataggacatg
     7261 aggctggacc cagaggccat aaagccatgg gtcacaggtc aggatattgg gtagcatcag
     7321 agggtgacac gaactccaag gatggtccag agcgagccag ggaaaccagg cttgtggatg
     7381 gggcaggacc tggggtggaa cctgggatgg ctggaatgcc aggcactgca ggtggcatgg
     7441 cacacagaga cagcctcagg ggcacagggg tgctggggtc tcagggaggg cgacagactc
     7501 tttcagatga gcgaggctcc accaaagatc ttgggggcta tggaacttca gggatccctg
     7561 aggcctcgga ggctgctggt gccaagggaa aaccagatgt caaagaatgg caagacagtt
     7621 ctgggactcc agggtcttct agagacagag gggctcccag ggtgaaggat aggtctccag
     7681 accaagcagg gataatgggg gcttctgggt ttcttgatgg caagggggca gtggaaggtg
     7741 agacctgggc aggaatggct gctctagggt ctggatatga acgggacatc tggaaagcag
     7801 gcccaggaat gacagacagg ggtagagttg ctggccaggg ggggttggca tctcagggag
     7861 gtggggactc acttttggga ggcagaaggg taggctcagg gagttcagtg gggacaggtc
     7921 aggatctgga cagcggctct atgcctgggg gaaggggcaa gtcaacatca gggcctgctg
     7981 atagacaagg gacgagcaat gcttgggctc ctgattggga aaaccagggg tttagccaag
     8041 gcagcataga tgctgggaag cagcccgcag gctccagagc ttccggttct ctgcaggaga
     8101 aagatgccgc ttttggtggg acccatgaag ggccaggggg ctttaagggt ggggagggtg
     8161 caccaggcca agaggcggct ggtggatgcc gaagcccatg gtccctggat agcaaaggtt
     8221 caagtcctgg aaggggcagt tctgttgatg cagaggactc aggtatcctg ggcaagggga
     8281 attctactga gtgggggaat gccctcaccc caaaacctgg ggagtccgga cctcagggag
     8341 cctggaatgg cttagatggt ccctttggca gaaaagcctc tagagatagg tcaggaggga
     8401 cccaggacct gagctctcag cgaggcaagg gacagagagg aggaaagagg tccctcgggg
     8461 agcaggggtc cctggaggct gagaatggtg aggtccaggg tcctggggcc ctaaaggagg
     8521 atgaagggca gggagtggaa gaggctggga ggtcaggcag gaggcctggc tcactcagga
     8581 gcaggtctca ggcacagtca ggggctgagg ttggaggagg aaagagaagg ggagcagacg
     8641 aggctggaag catggggtgg cagcctatgg gagagaactg ggggtgcctg gaggagatgc
     8701 tgaatgaaga tcagagccgg gagccccctg gtcaccttgg tagcaggaga agtggcaaag
     8761 acggcaggtt ggacatctat ggagagagga gagatgctac ccggagttcc acatccagat
     8821 acaagcctgg cactggcagt ttctccaagg atgcccaagg ccccatgggc cacttctccc
     8881 agggcctggc tgacatggaa gtgcagccgg gggaggccgc cacactctcc tgtaccctca
     8941 ccagtgacct gggacctggc acctggttta aggatggcgt caagctcacc acccaggatg
     9001 gagtcatctt taagcaagac ggtctcgtgc acagcctctt catcacgcat gtgcagggga
     9061 cccaagctgg gaggtacacc tttgtagctg gtgaccagca gagcgaggcc accctgaccg
     9121 tccaggattc ccctaccatt gctccagatg tgacagagaa actgagagag ccactggtgg
     9181 tcaaggctgg gaagccggtg atagtgaaga tccccttcca gagccacctc cccattcagg
     9241 ctgcctggag gaaggacggg gctgaggtgg tgggcagcag tgacagggag gcccaggtgg
     9301 acctggggga tggctacacg cggctgtgcc tccccagcgc aggcaggaag gactgtggcc
     9361 agtacagcgt gacactgagg agtgagggag gctctgtgca ggccgagctc actctgcaag
     9421 tcatagacaa gcctgatccc ccacaaggcc ccatggaggt tcaggattgc catagggctg
     9481 gcgtctgcct ccgctggcgg cccccaaggg acaatggggg ccggactgta gagtgctacg
     9541 tggtggagag acggcaggct ggcaggagca cttggctgaa ggtgggcgag gcccccgctg
     9601 acagcaccac cttcacggat gcccatgtgg agccaggcag gaagtatacc ttccgagtgc
     9661 gggctgtgac ctcagagggg gctggcgagg ccctggagtc tgaggagata ttggtggctc
     9721 ctgaggctct ccccaaggcc ccttccgcgc cagccatcct gtcggcctcc agccagggca
     9781 tcacactgac atggacagca cctcggggcc ccggcagcgc ccacatcctg ggctacctga
     9841 tcgagaggcg taagaagggg agcaacacct ggacggcagt gaacgaccag ccggtgcctg
     9901 agaggaggtg gacggtggcg gacgtgcggc agggctgtca gtatgagttc cgggtcacag
     9961 ctgtggctcc ctcaggtccc ggagagcctg gacctccatc ggatgctgtc tttgctcggg
    10021 accccatgag accccctggg ctggtgagga atctccaagt cacagacaga tcgaacacca
    10081 gcatcactct gagctgggct gggccagaca cccaggaagg ggatgaagcc caggggtatg
    10141 tggtggagct gtgcagctca gacagtctcc agtggctccc gtgccatgtg ggcaccgtgc
    10201 cagtcaccac ctacacggcc aaggggcttc ggcctggaga gggctacttc gtgcgggtga
    10261 cagcagttaa tgaaggaggc cagagccagc ccagtgccct ggacacatta gtgcaagcca
    10321 tgcctgttac tgtctgtccc aagttcctcg tggactccag caccaaggac ttgctgacag
    10381 tcaaggtcgg ggacacagtt cgtgtgcccg tctcctttga agccatgccc atgcctgagg
    10441 tgacctggct gaaggatggc ttgcccttgc ccaaaagaag tgtgactgtc actaaggatg
    10501 gcctcaccca gcttctgatc cctgtggctg gactctcaga cagtggtctc tacactgtgg
    10561 tgctgaggac cctgcagggg aaggaggttg cccacagctt ccgtatcagg gtggcagcat
    10621 gcccgcaggc ccctgggccc atccacctgc aggagaacgt gcctgggacg gtgacggccg
    10681 agtgggaacc ctctcctgac gaggcccagg atgtcccgct gcactacgcg gtgttcacac
    10741 gctcctcagc gcacggtccc tggcacgagg cagccgaccg catccacacc aaccgcttca
    10801 ccctcctggg catcctcccc ggccacgaat accacttcag ggtggtggcc aagaatgagc
    10861 tgggggccag caaaccctcg gacaccagcc agccctggtg catcccccgg cagcgcgaca
    10921 ggttcacagt gaaggctccg tgctaccggg agcccgacct gagccagaag ccccggttcc
    10981 tggtgggcct gcggtcccac ctgctgcccc agggctgcga gtgctgcatg agctgtgccg
    11041 tgcagggctc gccccggccc cacgtcacct ggttcaagaa tgaccgcagc ctggaaggaa
    11101 accccgcggt gtacagcact gacctgctgg gcgtgtgctc cctcaccatc cccagcgtat
    11161 ccccgaagga cagcggggag tacaaggctg tggctgagaa cacgctgggc caggcagtca
    11221 gcactgccac cctcattgtc atagaaccca gcacctagcc tcacctcacc ctgggatggt
    11281 cctggaccct tgaagcttca cttccgacac ctgcactggc ccgggaagcc aatcccaagg
    11341 atggaggctg tgcccagagc cccaggaaaa tgggagtgag aagatgcctg gcgaggtttt
    11401 ggccaggagg acgtgaagtc cttggggaag aaaaacaagg gaggagggca ttccacctcc
    11461 tggctccaag ctaagtcact caacagaatg acagcgaacc tcctggaccc tcaccatatg
    11521 ggtttctttc ttcttcgctt caggagatcc agagggcacc tgcctgcagg atgggccggc
    11581 tccttatttt cctgggctga gccgtttgga gggagggtgg gcacagctgg gcctgctgtg
    11641 accactggga gggaagagga gaggaggtca gaggacctgg gtgaggacta gggcatcaag
    11701 gtcgtgtgtg tgggtggggg cagcctccac ctcctggatt caagagattc caggggtgca
    11761 gcaagtgctt tctatgcgtt tcttcattaa aacattaggc taatacaggc a
//
