LOCUS       NM_014714               5232 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens intraflagellar transport 140 (IFT140), mRNA.
ACCESSION   NM_014714 XM_005255724
VERSION     NM_014714.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Salhi,S., Doreille,A., Dancer,M.S., Boueilh,A., Filipozzi,P., El
            Karoui,K., Ponce,F., Lebre,A.S., Raymond,L. and Mesnard,L.
  TITLE     Monoallelic Loss-of-Function IFT140 Pathogenic Variants Cause
            Autosomal Dominant Polycystic Kidney Disease: A Confirmatory Study
            With Suspicion of an Additional Cardiac Phenotype
  JOURNAL   Am J Kidney Dis 83 (5), 688-691 (2024)
   PUBMED   37844724
  REMARK    GeneRIF: Monoallelic Loss-of-Function IFT140 Pathogenic Variants
            Cause Autosomal Dominant Polycystic Kidney Disease: A Confirmatory
            Study With Suspicion of an Additional Cardiac Phenotype.
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Senum,S.R., Li,Y.S.M., Benson,K.A., Joli,G., Olinger,E., Lavu,S.,
            Madsen,C.D., Gregory,A.V., Neatu,R., Kline,T.L., Audrezet,M.P.,
            Outeda,P., Nau,C.B., Meijer,E., Ali,H., Steinman,T.I., Mrug,M.,
            Phelan,P.J., Watnick,T.J., Peters,D.J.M., Ong,A.C.M., Conlon,P.J.,
            Perrone,R.D., Cornec-Le Gall,E., Hogan,M.C., Torres,V.E.,
            Sayer,J.A. and Harris,P.C.
  CONSRTM   Genomics England Research Consortium, the HALT PKD, CRISP, DIPAK,
            ADPKD Modifier, and TAME PKD studies
  TITLE     Monoallelic IFT140 pathogenic variants are an important cause of
            the autosomal dominant polycystic kidney-spectrum phenotype
  JOURNAL   Am J Hum Genet 109 (1), 136-156 (2022)
   PUBMED   34890546
  REMARK    GeneRIF: Monoallelic IFT140 pathogenic variants are an important
            cause of the autosomal dominant polycystic kidney-spectrum
            phenotype.
REFERENCE   3  (bases 1 to 5232)
  AUTHORS   Wang,X., Sha,Y.W., Wang,W.T., Cui,Y.Q., Chen,J., Yan,W., Hou,X.T.,
            Mei,L.B., Yu,C.C. and Wang,J.
  TITLE     Novel IFT140 variants cause spermatogenic dysfunction in humans
  JOURNAL   Mol Genet Genomic Med 7 (9), e920 (2019)
   PUBMED   31397098
  REMARK    GeneRIF: This study for the first time reported IFT140 variants
            that cause infertility in humans.
REFERENCE   4  (bases 1 to 5232)
  AUTHORS   Mukhopadhyay,S., Wen,X., Chih,B., Nelson,C.D., Lane,W.S.,
            Scales,S.J. and Jackson,P.K.
  TITLE     TULP3 bridges the IFT-A complex and membrane phosphoinositides to
            promote trafficking of G protein-coupled receptors into primary
            cilia
  JOURNAL   Genes Dev 24 (19), 2180-2193 (2010)
   PUBMED   20889716
REFERENCE   5  (bases 1 to 5232)
  AUTHORS   Nakayama,M., Kikuno,R. and Ohara,O.
  TITLE     Protein-protein interactions between large proteins: two-hybrid
            screening using a functionally classified library composed of long
            cDNAs
  JOURNAL   Genome Res 12 (11), 1773-1784 (2002)
   PUBMED   12421765
REFERENCE   6  (bases 1 to 5232)
  AUTHORS   Daniels,R.J., Peden,J.F., Lloyd,C., Horsley,S.W., Clark,K.,
            Tufarelli,C., Kearney,L., Buckle,V.J., Doggett,N.A., Flint,J. and
            Higgs,D.R.
  TITLE     Sequence, structure and pathology of the fully annotated terminal 2
            Mb of the short arm of human chromosome 16
  JOURNAL   Hum Mol Genet 10 (4), 339-352 (2001)
   PUBMED   11157797
REFERENCE   7  (bases 1 to 5232)
  AUTHORS   Kumaran,N., Pennesi,M.E., Yang,P., Trzupek,K.M., Schlechter,C.,
            Moore,A.T., Weleber,R.G. and Michaelides,M.
  TITLE     Leber Congenital Amaurosis / Early-Onset Severe Retinal Dystrophy
            Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   30285347
REFERENCE   8  (bases 1 to 5232)
  AUTHORS   Tan,W., Lin,A. and Keppler-Noreuil,K.
  TITLE     Cranioectodermal Dysplasia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24027799
REFERENCE   9  (bases 1 to 5232)
  AUTHORS   Fahim,A.T., Daiger,S.P. and Weleber,R.G.
  TITLE     Nonsyndromic Retinitis Pigmentosa Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301590
REFERENCE   10 (bases 1 to 5232)
  AUTHORS   Harris,P.C. and Torres,V.E.
  TITLE     Polycystic Kidney Disease, Autosomal Dominant
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301424
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC401677.1, BC035577.1 and
            AW245075.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_014714.3.
            
            Summary: This gene encodes one of the subunits of the
            intraflagellar transport (IFT) complex A. Intraflagellar transport
            is involved in the genesis, resorption and signaling of primary
            cilia. The primary cilium is a microtubule-based sensory organelle
            at the surface of most quiescent mammalian cells, that receives
            signals from its environment, such as the flow of fluid, light or
            odors, and transduces those signals to the nucleus. Loss of the
            corresponding protein in mouse results in renal cystic disease.
            [provided by RefSeq, Jun 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.1630287.1,
                                           SRR14038196.2506225.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000426508.7/ ENSP00000406012.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-95                DC401677.1         44-138
            96-295              DC401677.1         140-339
            296-5230            BC035577.1         17-4951
            5231-5232           AW245075.1         10-11               c
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..5232
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /note="intraflagellar transport 140"
                     /db_xref="GeneID:9742"
                     /db_xref="HGNC:HGNC:29077"
                     /db_xref="MIM:614620"
     exon            1..105
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            106..295
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            296..473
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             327..4715
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /note="WD and tetratricopeptide repeats protein 2;
                     intraflagellar transport 140 homolog"
                     /codon_start=1
                     /product="intraflagellar transport protein 140 homolog"
                     /protein_id="NP_055529.2"
                     /db_xref="CCDS:CCDS10439.1"
                     /db_xref="GeneID:9742"
                     /db_xref="HGNC:HGNC:29077"
                     /db_xref="MIM:614620"
                     /translation="MALYYDHQIEAPDAAGSPSFISWHPVHPFLAVAYISTTSTGSVD
                     IYLEQGECVPDTHVERPFRVASLCWHPTRLVLAVGWETGEVTVFNKQDKEQHTMPLTH
                     TADITVLRWSPSGNCLLSGDRLGVLLLWRLDQRGRVQGTPLLKHEYGKHLTHCIFRLP
                     PPGEDLVQLAKAAVSGDEKALDMFNWKKSSSGSLLKMGSHEGLLFFVSLMDGTVHYVD
                     EKGKTTQVVSADSTIQMLFYMEKREALVVVTENLRLSLYTVPPEGKAEEVMKVKLSGK
                     TGRRADIALIEGSLLVMAVGEAALRFWDIERGENYILSPDEKFGFEKGENMNCVCYCK
                     VKGLLAAGTDRGRVAMWRKVPDFLGSPGAEGKDRWALQTPTELQGNITQIQWGSRKNL
                     LAVNSVISVAILSERAMSSHFHQQVAAMQVSPSLLNVCFLSTGVAHSLRTDMHISGVF
                     ATKDAVAVWNGRQVAIFELSGAAIRSAGTFLCETPVLAMHEENVYTVESNRVQVRTWQ
                     GTVKQLLLFSETEGNPCFLDICGNFLVVGTDLAHFKSFDLSRREAKAHCSCRSLAELV
                     PGVGGIASLRCSSSGSTISILPSKADNSPDSKICFYDVEMDTVTVFDFKTGQIDRRET
                     LSFNEQETNKSHLFVDEGLKNYVPVNHFWDQSEPRLFVCEAVQETPRSQPQSANGQPQ
                     DGRAGPAADVLILSFFISEEHGFLLHESFPRPATSHSLLGMEVPYYYFTRKPEEADRE
                     DEVEPGCHHIPQMVSRRPLRDFVGLEDCDKATRDAMLHFSFFVTIGDMDEAFKSIKLI
                     KSEAVWENMARMCVKTQRLDVAKVCLGNMGHARGARALREAEQEPELEARVAVLATQL
                     GMLEDAEQLYRKCKRHDLLNKFYQAAGRWQEALQVAEHHDRVHLRSTYHRYAGHLEAS
                     ADCSRALSYYEKSDTHRFEVPRMLSEDLPSLELYVNKMKDKTLWRWWAQYLESQGEMD
                     AALHYYELARDHFSLVRIHCFQGNVQKAAQIANETGNLAASYHLARQYESQEEVGQAV
                     HFYTRAQAFKNAIRLCKENGLDDQLMNLALLSSPEDMIEAARYYEEKGVQMDRAVMLY
                     HKAGHFSKALELAFATQQFVALQLIAEDLDETSDPALLARCSDFFIEHSQYERAVELL
                     LAARKYQEALQLCLGQNMSITEEMAEKMTVAKDSSDLPEESRRELLEQIADCCMRQGS
                     YHLATKKYTQAGNKLKAMRALLKSGDTEKITFFASVSRQKEIYIMAANYLQSLDWRKE
                     PEIMKNIIGFYTKGRALDLLAGFYDACAQVEIDEYQNYDKAHGALTEAYKCLAKAKAK
                     SPLDQETRLAQLQSRMALVKRFIQARRTYTEDPKESIKQCELLLEEPDLDSTIRIGDV
                     YGFLVEHYVRKEEYQTAYRFLEEMRRRLPLANMSYYVSPQAVDAVHRGLGLPLPRTVP
                     EQVRHNSMEDARELDEEVVEEADDDP"
     exon            474..695
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            696..817
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            818..960
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1136
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1228
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1335
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1481
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1685
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..1758
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1850
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..1978
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..2096
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2097..2227
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2228..2393
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..2525
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2526..2725
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2726..2903
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2904..3094
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3095..3190
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3191..3323
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3324..3467
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3468..3596
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3597..3779
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3780..3986
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3987..4199
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            4200..4366
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            4367..4508
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     exon            4509..5232
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5209..5214
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /note="hexamer: AATAAA"
     polyA_site      5232
                     /gene="IFT140"
                     /gene_synonym="c305C8.4; c380F5.1; gs114; MZSDS; RP80;
                     SRTD9; WDTC2"
                     /note="major polyA site"
ORIGIN      
        1 gcagcagtgt tgacggatac gtgagaagaa aacggcgtgg tagctcggag tgtggaatcg
       61 ggcgcgaacc tgagttctga gaagtgtgga agcacgtctg aggcaagcgg aataacctgg
      121 cccccgacgc tcgggcctcg gagctgcacg tgcgtgcccg atcgccttcg gctctttccg
      181 gccgctcggg gcgcttcggg agtcctcggg gccgcctcgc tcaccttcgg ctatttccgc
      241 gttcctggcg cgccgttgtc ccggcaacgc gcggcggctc agcggcctgg cagaggtttc
      301 agcgctgagg aggcctgagt tccgtcatgg ccctctatta tgaccaccag atagaagccc
      361 cggatgcagc agggtcaccc tcatttatca gctggcaccc tgtccatcca ttcttggcag
      421 ttgcttacat cagcacaacc tcaacaggca gcgtggatat ttacctggag caaggggagt
      481 gcgtgccaga tacacacgtc gagaggccgt tccgggttgc ttccctgtgc tggcacccga
      541 cgcggctggt gctggctgtg ggctgggaga ctggagaagt gacggtgttt aacaagcagg
      601 acaaggagca gcacacgatg cccctgacac acacagccga catcaccgtg ctccgttgga
      661 gccccagtgg aaactgcctg ctgtctgggg acaggcttgg tgtcttgctc ttgtggaggt
      721 tggaccaaag gggccgagtg caagggacgc ctctgctgaa acacgagtat gggaaacacc
      781 tcacgcactg catcttccgg ctcccccctc ctggcgagga cctggttcag ttggcaaagg
      841 cagctgtgag cggtgatgag aaagccctgg acatgtttaa ctggaagaag agcagttctg
      901 gaagtttgct gaagatgggg tctcacgagg ggctgttgtt ctttgtcagt ctgatggacg
      961 ggacagtgca ctatgtggat gagaagggca agaccactca ggtggtgtcc gcagacagca
     1021 cgattcagat gctgttctac atggagaaga gggaggcact ggtggtggtc acagagaacc
     1081 tccggctgtc cctgtacacg gtgcctcctg agggcaaagc agaagaagtg atgaaggtca
     1141 agctgagcgg gaaaaccggc cgccgggcag acatcgcttt gattgaaggc agccttctcg
     1201 tgatggccgt cggggaggct gccctcagat tctgggacat agaacgagga gagaattata
     1261 tactgagtcc agatgagaag tttggctttg agaaaggaga gaatatgaac tgtgtgtgtt
     1321 actgtaaagt caaaggtctt ctggccgctg gtaccgacag agggcgagta gccatgtgga
     1381 ggaaagtacc agacttcctg ggcagccccg gggcagaggg caaggacagg tgggcccttc
     1441 agacccctac cgagctccaa ggaaacatca cgcaaatcca gtggggttcc aggaagaacc
     1501 tgctggcagt gaacagcgtc atctccgtgg ccatcctcag cgagcgggcc atgtcgtcac
     1561 acttccacca gcaagtggcc gccatgcagg tctccccgag tctgctgaat gtgtgcttcc
     1621 tgtccacggg ggtcgcacac agcctgcgca ccgacatgca catcagtgga gtgtttgcca
     1681 ccaaggatgc tgtcgcagtc tggaacggaa ggcaggtggc gatcttcgag ctttctggag
     1741 ccgcgatacg gagtgcaggg accttcttgt gtgagacgcc tgtgttagca atgcatgaag
     1801 aaaacgttta cacggtggag tcaaaccgag ttcaagttcg aacctggcag gggactgtca
     1861 aacaactcct ccttttctcg gagactgagg ggaatccctg cttcttggac atctgtggga
     1921 atttcctggt tgtagggaca gacttggctc actttaaaag ctttgatctt tcccgaagag
     1981 aggccaaagc acactgtagc tgcaggagcc tggcggagct ggtccctggg gtggggggca
     2041 tcgcttctct gcggtgcagc agcagcggga gcaccatcag catcctcccc agcaaggctg
     2101 acaacagccc tgattccaaa atctgcttct acgatgttga aatggacaca gtgaccgtct
     2161 ttgacttcaa gactggacaa attgatcgga gagagacgct gtcctttaat gagcaagaga
     2221 ctaataagag ccacctcttt gtggatgagg gactgaaaaa ttatgttccc gtgaaccact
     2281 tctgggacca gagtgagccc cggctgtttg tatgcgaagc cgtgcaggag acgccgcgct
     2341 cccagcctca gtctgcaaac gggcagcccc aagatgggcg cgctggccct gcggcagatg
     2401 ttttgatcct gtccttcttc atttccgaag agcacggctt cctgcttcat gagagcttcc
     2461 cccggcctgc cacctcccac agtctcctgg ggatggaagt gccttattac tacttcacaa
     2521 gaaagcccga agaagcagac agagaagacg aggtggagcc tgggtgccac cacatccctc
     2581 agatggtgtc caggagaccc ctgcgagact ttgtggggct ggaggactgc gacaaggcca
     2641 cccgggacgc catgctccac ttcagcttct ttgtcaccat aggagacatg gacgaagcct
     2701 tcaaatccat caagctcatc aaaagtgagg ccgtctggga gaacatggcg cgcatgtgcg
     2761 tgaagaccca gcggctggac gtggccaagg tgtgcctggg gaacatgggc catgcccgcg
     2821 gggcccgagc gctgcgtgag gcggagcagg agccggagct agaggcccgc gtggccgtgc
     2881 tggccacgca gctgggcatg ctggaggacg ccgagcagct gtacaggaag tgcaagcgcc
     2941 acgacctcct gaacaagttc taccaggctg cgggccggtg gcaggaggcc ctccaggtag
     3001 ccgagcacca cgatcgcgtg cacctgcgca gcacctacca ccgctatgcc gggcacctgg
     3061 aggccagcgc cgactgcagc cgggccctca gttactacga gaagtcggac acgcaccgct
     3121 tcgaggtgcc caggatgctg tcggaggacc tgccgtccct ggagctctac gtgaataaaa
     3181 tgaaggataa gaccctgtgg cggtggtggg cgcagtacct ggagagccag ggcgagatgg
     3241 acgccgcgct gcactactac gagctggccc gggaccactt ctccctggtc cgcatccact
     3301 gcttccaggg caatgtccag aaggctgcgc aaatagccaa cgagacagga aacctggcgg
     3361 cctcctacca cctcgcccgc cagtacgaga gccaggagga ggtcgggcag gcggtgcact
     3421 tctacacccg ggcacaggcc ttcaagaatg ccatccgcct gtgcaaggag aacggcctgg
     3481 acgaccagct catgaacttg gccctgctga gctcccccga ggacatgatc gaggcggccc
     3541 gatactacga ggagaagggc gtgcagatgg acagggcggt catgctgtac cacaaggctg
     3601 gccacttctc caaggccctg gagctggcct ttgccaccca gcagtttgtg gccctacagc
     3661 tcatagcaga ggacctggat gagacgtcag accctgcgct cctggcccgc tgctccgact
     3721 tcttcatcga gcacagtcag tacgagaggg cggtagagct gctgctggct gccaggaagt
     3781 atcaggaagc cctgcagctg tgcctggggc agaacatgag catcaccgag gagatggcgg
     3841 aaaagatgac cgtggccaag gactcctcgg acctgcctga ggagtcgcgg cgggagctgc
     3901 tggagcagat agcagactgc tgcatgcgcc agggcagcta ccacctggcc accaagaagt
     3961 acacgcaggc cggcaacaag ctgaaggcca tgagggcgct gctcaaatcc ggagacacgg
     4021 agaaaatcac gttcttcgcg agcgtgtcca ggcagaagga aatctacatc atggctgcta
     4081 actacctgca gtccctggac tggcggaagg agccggagat catgaagaac atcatcggct
     4141 tctacaccaa ggggcgggcc ctggacctcc tggctggctt ttatgacgct tgtgcccagg
     4201 tggagattga tgaataccag aactacgaca aagcccacgg ggcgctgacc gaggcctaca
     4261 agtgcctggc caaggccaag gccaagagcc ccctggacca ggagaccagg ctggcgcagc
     4321 tgcagagcag gatggcactg gtgaagaggt tcatccaggc ccgcaggacg tacacagagg
     4381 accccaagga gtccatcaag cagtgtgagc tgctcctgga ggaaccagac ctggacagca
     4441 ccatccgcat cggggacgtc tatggcttcc tggtggagca ctacgtgcgg aaggaggaat
     4501 accagacggc ctacagattc ctggaggaga tgcggcggcg gcttcccttg gccaacatgt
     4561 cctactacgt gagcccgcag gccgtggacg ccgtgcaccg ggggctgggt ctcccactgc
     4621 cacgcaccgt ccccgagcag gtccgccaca acagcatgga ggacgccagg gagctggacg
     4681 aggaggtggt ggaagaggca gatgacgacc cctgaggggc ctgggcccca ggaccagcgt
     4741 gctgctgcag aaaggcatct tctggaattt ttttgtcagc tgtggcaaag ccagcatttt
     4801 tgctgggaaa aaacatgtct gtgttggaat acgcgacaga gctgggcgag aacgcagcgg
     4861 cccgggccgg cggagggtgt gacccgtctg caccttgctc tgtcccacct gcctctgggt
     4921 gcccggcagc tccactagat ttttggattc attcctttga agggagtcgg gttcaccctt
     4981 ccatcgtatt ctcccaacta cacattgtaa agcctgagaa acttctagaa cctcaggaag
     5041 ctgcagctgg agggctgggg cacctgcccc cctgctcccc acacatcata tcctccccat
     5101 actcctgcag ggcccacggc tcctgagcaa cagctgggac acccgggcct tggcggctgc
     5161 accccctgct aggctctgcc caccggccac caacactcct gtaattccaa taaagcagtt
     5221 tattttctga ga
//
