LOCUS       NM_000202               7580 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens iduronate 2-sulfatase (IDS), transcript variant 1,
            mRNA.
ACCESSION   NM_000202
VERSION     NM_000202.8
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Ramirez-Hernandez,M.A., Figuera,L.E., Rizo-de la Torre,L.C.,
            Mendoza-Ruvalcaba,M.T.M.S.C., Arnaud-Lopez,L., Garcia-Ortiz,J.E.,
            Zuniga-Gonzalez,G.M., Puebla-Perez,A.M., Gomez-Meda,B.C. and
            Gallegos-Arreola,M.P.
  TITLE     Mutational spectrum of the iduronate-2-sulfatase gene in Mexican
            patients with Hunter syndrome
  JOURNAL   Eur Rev Med Pharmacol Sci 26 (14), 5115-5127 (2022)
   PUBMED   35916809
  REMARK    GeneRIF: Mutational spectrum of the iduronate-2-sulfatase gene in
            Mexican patients with Hunter syndrome.
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Zhao,X.Y., Qiao,G.M. and Liu,F.
  TITLE     Identification and structure characterization of novel IDS variants
            causing mucopolysaccharidosis type II: A retrospective analysis of
            30 Chinese children
  JOURNAL   Clin Chim Acta 523, 386-394 (2021)
   PUBMED   34670126
  REMARK    GeneRIF: Identification and structure characterization of novel IDS
            variants causing mucopolysaccharidosis type II: A retrospective
            analysis of 30 Chinese children.
REFERENCE   3  (bases 1 to 7580)
  AUTHORS   Matsuhisa,K. and Imaizumi,K.
  TITLE     Loss of Function of Mutant IDS Due to Endoplasmic
            Reticulum-Associated Degradation: New Therapeutic Opportunities for
            Mucopolysaccharidosis Type II
  JOURNAL   Int J Mol Sci 22 (22), 12227 (2021)
   PUBMED   34830113
  REMARK    GeneRIF: Loss of Function of Mutant IDS Due to Endoplasmic
            Reticulum-Associated Degradation: New Therapeutic Opportunities for
            Mucopolysaccharidosis Type II.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7580)
  AUTHORS   Jezela-Stanek,A., Pokora,P., Mlynek,M., Smyk,M., Ziemkiewicz,K.,
            Rozdzynska-Swiatkowska,A. and Tylki-Szymanska,A.
  TITLE     Diverse clinical outcome of Hunter syndrome in patients with
            chromosomal aberration encompassing entire and partial IDS
            deletions: what is important for early diagnosis and counseling?
  JOURNAL   Clin Dysmorphol 30 (2), 76-82 (2021)
   PUBMED   33290290
  REMARK    GeneRIF: Diverse clinical outcome of Hunter syndrome in patients
            with chromosomal aberration encompassing entire and partial IDS
            deletions: what is important for early diagnosis and counseling?
REFERENCE   5  (bases 1 to 7580)
  AUTHORS   Scarpa,M. and Lampe,C.
  TITLE     Mucopolysaccharidosis Type II
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301451
REFERENCE   6  (bases 1 to 7580)
  AUTHORS   Crotty,P.L., Braun,S.E., Anderson,R.A. and Whitley,C.B.
  TITLE     Mutation R468W of the iduronate-2-sulfatase gene in mild Hunter
            syndrome (mucopolysaccharidosis type II) confirmed by in vitro
            mutagenesis and expression
  JOURNAL   Hum Mol Genet 1 (9), 755-757 (1992)
   PUBMED   1284597
REFERENCE   7  (bases 1 to 7580)
  AUTHORS   Beck,M., Steglich,C., Zabel,B., Dahl,N., Schwinger,E., Hopwood,J.J.
            and Gal,A.
  TITLE     Deletion of the Hunter gene and both DXS466 and DXS304 in a patient
            with mucopolysaccharidosis type II
  JOURNAL   Am J Med Genet 44 (1), 100-103 (1992)
   PUBMED   1355630
REFERENCE   8  (bases 1 to 7580)
  AUTHORS   Bunge,S., Steglich,C., Beck,M., Rosenkranz,W., Schwinger,E.,
            Hopwood,J.J. and Gal,A.
  TITLE     Mutation analysis of the iduronate-2-sulfatase gene in patients
            with mucopolysaccharidosis type II (Hunter syndrome)
  JOURNAL   Hum Mol Genet 1 (5), 335-339 (1992)
   PUBMED   1303211
REFERENCE   9  (bases 1 to 7580)
  AUTHORS   Sukegawa,K., Tomatsu,S., Tamai,K., Ikeda,M., Sasaki,T., Masue,M.,
            Fukuda,S., Yamada,Y. and Orii,T.
  TITLE     Intermediate form of mucopolysaccharidosis type II (Hunter
            disease): a C1327 to T substitution in the iduronate sulfatase gene
  JOURNAL   Biochem Biophys Res Commun 183 (2), 809-813 (1992)
   PUBMED   1550586
REFERENCE   10 (bases 1 to 7580)
  AUTHORS   Wilson,P.J., Morris,C.P., Anson,D.S., Occhiodoro,T., Bielicki,J.,
            Clements,P.R. and Hopwood,J.J.
  TITLE     Hunter syndrome: isolation of an iduronate-2-sulfatase cDNA clone
            and analysis of patient DNA
  JOURNAL   Proc Natl Acad Sci U S A 87 (21), 8531-8535 (1990)
   PUBMED   2122463
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA325538.1, M58342.1,
            BY996633.1, AK055600.1, DA351886.1, CN303532.1, AK057191.1,
            AC233288.1, CK004173.1 and R46390.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_000202.7.
            
            Summary: This gene encodes a member of the sulfatase family of
            proteins. The encoded preproprotein is proteolytically processed to
            generate two polypeptide chains. This enzyme is involved in the
            lysosomal degradation of heparan sulfate and dermatan sulfate.
            Mutations in this gene are associated with the X-linked lysosomal
            storage disease mucopolysaccharidosis type II, also known as Hunter
            syndrome. Alternative splicing results in multiple transcript
            variants, at least one of which encodes a preproprotein that is
            proteolytically processed. [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.154379.1,
                                           SRR1803612.252715.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000340855.11/ ENSP00000339801.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-105               DA325538.1         49-153
            106-2341            M58342.1           61-2296
            2342-2927           BY996633.1         142-727
            2928-3499           AK055600.1         367-938
            3500-4017           DA351886.1         32-549
            4018-4780           AK055600.1         1457-2219
            4781-5033           CN303532.1         410-662
            5034-5798           AK057191.1         1644-2408
            5799-7008           AC233288.1         54667-55876         c
            7009-7513           CK004173.1         46-550
            7514-7520           AC233288.1         54155-54161         c
            7521-7578           R46390.1           14-71               c
            7579-7580           AC233288.1         54095-54096         c
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq28"
     gene            1..7580
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /note="iduronate 2-sulfatase"
                     /db_xref="GeneID:3423"
                     /db_xref="HGNC:HGNC:5389"
                     /db_xref="MIM:300823"
     exon            1..272
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /inference="alignment:Splign:2.1.0"
     CDS             170..1822
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /EC_number="3.1.6.13"
                     /note="isoform a preproprotein is encoded by transcript
                     variant 1; iduronate 2-sulfatase 42 kDa chain; iduronate
                     2-sulfatase 14 kDa chain; alpha-L-iduronate sulfate
                     sulfatase; idursulfase"
                     /codon_start=1
                     /product="iduronate 2-sulfatase isoform a preproprotein"
                     /protein_id="NP_000193.1"
                     /db_xref="CCDS:CCDS14685.1"
                     /db_xref="GeneID:3423"
                     /db_xref="HGNC:HGNC:5389"
                     /db_xref="MIM:300823"
                     /translation="MPPPRTGRGLLWLGLVLSSVCVALGSETQANSTTDALNVLLIIV
                     DDLRPSLGCYGDKLVRSPNIDQLASHSLLFQNAFAQQAVCAPSRVSFLTGRRPDTTRL
                     YDFNSYWRVHAGNFSTIPQYFKENGYVTMSVGKVFHPGISSNHTDDSPYSWSFPPYHP
                     SSEKYENTKTCRGPDGELHANLLCPVDVLDVPEGTLPDKQSTEQAIQLLEKMKTSASP
                     FFLAVGYHKPHIPFRYPKEFQKLYPLENITLAPDPEVPDGLPPVAYNPWMDIRQREDV
                     QALNISVPYGPIPVDFQRKIRQSYFASVSYLDTQVGRLLSALDDLQLANSTIIAFTSD
                     HGWALGEHGEWAKYSNFDVATHVPLIFYVPGRTASLPEAGEKLFPYLDPFDSASQLME
                     PGRQSMDLVELVSLFPTLAGLAGLQVPPRCPVPSFHVELCREGKNLLKHFRFRDLEED
                     PYLPGNPRELIAYSQYPRPSDIPQWNSDKPSLKDIKIMGYSIRTIDYRYTVWVGFNPD
                     EFLANFSDIHAGELYFVDSDPLQDHNMYNDSQGGDLFQLLMP"
     exon            273..409
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /inference="alignment:Splign:2.1.0"
     exon            410..587
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /inference="alignment:Splign:2.1.0"
     exon            588..676
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /inference="alignment:Splign:2.1.0"
     exon            677..877
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /inference="alignment:Splign:2.1.0"
     exon            878..1048
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1175
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1349
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..7580
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5776..5781
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /note="hexamer: AATAAA"
     polyA_site      5799
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /note="major polyA site"
     regulatory      7558..7563
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
                     /note="hexamer: AATAAA"
     polyA_site      7580
                     /gene="IDS"
                     /gene_synonym="ID2S; MPS2; SIDS"
ORIGIN      
        1 actcgcgctg cggccagcgc ccgggcctgc gggcccgggc ggcggctgtg ttgcgcagtc
       61 ttcatgggtt cccgacgagg aggtctctgt ggctgcggcg gcggctgcta actgcgccac
      121 ctgctgcagc ctgtccccgc cgctctgaag cggccgcgtc gaagccgaaa tgccgccacc
      181 ccggaccggc cgaggccttc tctggctggg tctggttctg agctccgtct gcgtcgccct
      241 cggatccgaa acgcaggcca actcgaccac agatgctctg aacgttcttc tcatcatcgt
      301 ggatgacctg cgcccctccc tgggctgtta tggggataag ctggtgaggt ccccaaatat
      361 tgaccaactg gcatcccaca gcctcctctt ccagaatgcc tttgcgcagc aagcagtgtg
      421 cgccccgagc cgcgtttctt tcctcactgg caggagacct gacaccaccc gcctgtacga
      481 cttcaactcc tactggaggg tgcacgctgg aaacttctcc accatccccc agtacttcaa
      541 ggagaatggc tatgtgacca tgtcggtggg aaaagtcttt caccctggga tatcttctaa
      601 ccataccgat gattctccgt atagctggtc ttttccacct tatcatcctt cctctgagaa
      661 gtatgaaaac actaagacat gtcgagggcc agatggagaa ctccatgcca acctgctttg
      721 ccctgtggat gtgctggatg ttcccgaggg caccttgcct gacaaacaga gcactgagca
      781 agccatacag ttgttggaaa agatgaaaac gtcagccagt cctttcttcc tggccgttgg
      841 gtatcataag ccacacatcc ccttcagata ccccaaggaa tttcagaagt tgtatccctt
      901 ggagaacatc accctggccc ccgatcccga ggtccctgat ggcctacccc ctgtggccta
      961 caacccctgg atggacatca ggcaacggga agacgtccaa gccttaaaca tcagtgtgcc
     1021 gtatggtcca attcctgtgg actttcagcg gaaaatccgc cagagctact ttgcctctgt
     1081 gtcatatttg gatacacagg tcggccgcct cttgagtgct ttggacgatc ttcagctggc
     1141 caacagcacc atcattgcat ttacctcgga tcatgggtgg gctctaggtg aacatggaga
     1201 atgggccaaa tacagcaatt ttgatgttgc tacccatgtt cccctgatat tctatgttcc
     1261 tggaaggacg gcttcacttc cggaggcagg cgagaagctt ttcccttacc tcgacccttt
     1321 tgattccgcc tcacagttga tggagccagg caggcaatcc atggaccttg tggaacttgt
     1381 gtctcttttt cccacgctgg ctggacttgc aggactgcag gttccacctc gctgccccgt
     1441 tccttcattt cacgttgagc tgtgcagaga aggcaagaac cttctgaagc attttcgatt
     1501 ccgtgacttg gaagaggatc cgtacctccc tggtaatccc cgtgaactga ttgcctatag
     1561 ccagtatccc cggccttcag acatccctca gtggaattct gacaagccga gtttaaaaga
     1621 tataaagatc atgggctatt ccatacgcac catagactat aggtatactg tgtgggttgg
     1681 cttcaatcct gatgaatttc tagctaactt ttctgacatc catgcagggg aactgtattt
     1741 tgtggattct gacccattgc aggatcacaa tatgtataat gattcccaag gtggagatct
     1801 tttccagttg ttgatgcctt gagttttgcc aaccatggat ggcaaatgtg atgtgctccc
     1861 ttccagctgg tgagaggagg agttagagct ggtcgttttg tgattaccca taatattgga
     1921 agcagcctga gggctagtta atccaaacat gcatcaacaa tttggcctga gaatatgtaa
     1981 cagccaaacc ttttcgttta gtctttatta aaatttataa ttggtaattg gaccagtttt
     2041 ttttttaatt tccctctttt taaaacagtt acggcttatt tactgaataa atacaaagca
     2101 aacaaactca agttatgtca tacctttgga tacgaagacc atacataata accaaacata
     2161 acattataca caaagaatac tttcattatt tgtggaattt agtgcatttc aaaaagtaat
     2221 catatatcaa actaggcacc acactaagtt cctgattatt ttgtttataa tttaataata
     2281 tatcttatga gccctatata ttcaaaatat tatgttaaca tgtaatccat gtttcttttt
     2341 caaatctaaa gttaaaaaaa aatagcagaa gccagtgtct taaagtctat cttttgtttc
     2401 taagaccatg ggatttcata atctcaagat aaaatatgta tgaagtaatt aatgtagaat
     2461 ttttacacca aataataaat aatgcttaat aaactagaga tatgagatgt gtaggaaatt
     2521 tggttaaact tttttcagat actttctggc ccaaataata atttgttagc aaataatatg
     2581 acccttgaac tcaatggcca tctattaaaa gactgttgtt cacactggaa aacatttaaa
     2641 gatgtgacta tatccatggg tggattgaat cactcaaaat atattagtat ccttctttag
     2701 ggatggttgg ttacagacat gtatttattc aggaggcaga aaatattcca ttttaattgc
     2761 ttattaaaga aaacattaaa ttctaaatta ttttgaggac tgtgaagact tttcattagt
     2821 gtaatattag gtcattgtca atctcccaga atgtagttct atattctcta aatatgaaag
     2881 tatccagaaa ggccagtggt agtaaaaagc ttagtgtata taatctcaaa agggatggaa
     2941 tatttacaac tcatatttat aacatgttga atcttctcag ttatcagtag tcatcagaag
     3001 tgtcaatagc tttctaaata aatattaaat atctactgtc ctgtagtgaa ggagtaattt
     3061 ttagtaattt tctctttaca aagtctccag tgtttccagg taaatatttg tgaaacaaaa
     3121 tacagcaaac tacattgtta cttcagtgta ttgttgccaa aaatgacaag atattatatt
     3181 aaaatcagta aattttagac agattttaaa aattaattag cctacaatag aggttatatg
     3241 gtaacacggt gatcttctaa gcagttaagt gactgactgt tctggcaaca acgacttctc
     3301 cgtgactgaa gggccctgtt catttcctga tcctgaagct cgtctctctt ttgagcctcc
     3361 gcttgctttg gtcgatggtt tccctcagct ttttctttgc tgttcttcat cctcgttgtt
     3421 gctgtcatca tgttcactgt ggcttttaca atacagcctg taaattcctt atgacatagt
     3481 tcagtgcatt tggctttatt gcctgctcca cagttcttta cctttacttg gcttagagaa
     3541 actgtatctt tgttgcttca tataaccttt ccccaacccc actaagctgg acataactta
     3601 ttagtggtcc tcccgtcact ttatttgtag aaatctctct ttcacatgag caggggttct
     3661 ttcatgtggt ttagctgaca gcagaactag tgattctaga cattttgcat ggccctcatt
     3721 cagtggctca caaacatgag ggagcatcag aactacttga ggggcttgtt aaaacccagt
     3781 gcgttagaag tcggatgcgg tggctcacac ctgtaatccc agcactttgg gaggcccagg
     3841 caggcggatc acttgaggtt aggagttcaa gaccagcctg gccaacatgg tgaaaccccg
     3901 tctctactaa aaatacaaaa gttagccggg tgtggtggtg catgcctgta atcccagctt
     3961 cttgggaggc caaggcacaa gaatcgcttg aaccaggaga cggaggtttc agtgaatgaa
     4021 gatcgtgcca ttgtattcca gcctcggcaa cacagcagga ctgtgatttt ctttggagac
     4081 tcctagattt tctgtggttt tgaactgaat ttgttggatg ttggcaagtg cctcttatga
     4141 gctgtttctt tatcctgcat ttgccccaca aagacttatc tggaggtgag caaagtatgt
     4201 ttggtagtga ggtcacaaag gcaatcagcc ccttcctccc cactcccatt gccatcttct
     4261 cagtccttct ccctttcttt ccaagtagtt tacccacccc tcctctttcc tcccctgtcc
     4321 ctaaaataat ccacgtgtct tcctaaaatc tctctttgat cctgtccttt gataacaccg
     4381 tcagtgccta ctactgggtc tagacagacc tctgttgagc agtcagagtc ttccctgact
     4441 ccacaatgcc cctttccttg gctgaccagt atgactactg gtccccacct ttcccttgcc
     4501 tatccctacc tccctcctac taggttgtcc catccctctc ttcacccatt cattcatgac
     4561 catttttcac taccaagctc cccccctccc gaaggaggct gaggtttttg tgactctcta
     4621 gactctattg tgggatggaa tgaacattgc taaagaatct tgtgttcgct ttactttaaa
     4681 aaggtatttt tttcctaatt ataaaactga tgtgtcagtt acggaaaaat tagaaatgca
     4741 gcacaaatac atgaatattt taccacaaaa ttgccatata atatcttgtc ttttttgggg
     4801 gtgtgaattt tttgcattgt tctggtcata ttctttatca tgtaatttat gttctttttt
     4861 actaagtatt atgtgtggtt attatagatt ttcacaaaga tatattgctg gtaatatatt
     4921 ttattgtgta gtcttataat ttacttaacc ttctttcaat tgttagaaat ttaggctatt
     4981 tccagatttt cagtattgta aataatgctg tgatgaccaa ttttgtgaat aaaatgtttt
     5041 tatgtatttc agattattcc cttaggatag tctctcagtg ccaagttgtc aaaaacatct
     5101 ctattttgct tatcttcctg ctctcttgct gccttagggg gtagtaaact gaaacataaa
     5161 gtaaacatgc atacaaataa aaaacataaa acaaaaataa gcaacctgat ggtaataggt
     5221 gaaagtggta acctgtttta actttgaatt cttgccgggc gcggtggctc acgcctgtaa
     5281 tcccagcact ttgggaggct gaggcgggtg gatcacgagg tcaggagttc aaaaccagcc
     5341 tggccaagat ggtgaaatcc cgtctctact aaaaatacaa aaattagccg ggcgtggtgg
     5401 cgggcgcctg taatcccagc tacttgggag gctgaggcag agaattgctt gaacccagga
     5461 ggcggaggtt gcagtgagcc aagatcgcgc cactgcactc cagcctgggt gacagagcga
     5521 gactccgtct caaataaaaa acaacaaaaa acaaaaaaaa cttaaaattc tttgcttgtt
     5581 agtgaccttg atcatggttc tctttgtacg atagttgggc atctgtattt ccacttgtgt
     5641 gaatttgcct ttaaattttg gttatgggtt tcacctttta aaataatcaa acatatttat
     5701 cttttcctgt gtgataggtt tttttctgta tcttttcctg ttaaacacac agacccctcc
     5761 ccaatctgga cattgaataa atattcattt tcctttgcat tgtttggggt ttggttttat
     5821 gcttactgtg tctctccacc ccattatcag agtaatatat gctcattgca taaaatctgg
     5881 aacatgtgaa aactggagat gaaaagaagc ccagcatttg agacataccc actgctgaca
     5941 tttgtatatt tcctttcatt cttttacctg cctaaagaca tatatgcaga tatggacata
     6001 tttagcttta catacatgca gactaccaca tataatctat atcttgtttt tttgatctaa
     6061 cacaatgtta gcttggtttt ttatgtcatt gacagttctt gattaacagc tgtaatggct
     6121 gcacagccct gcttcattaa tttttttttt tggactgaaa tgtatgtacc acgttccgct
     6181 tatccattca tccaccagtg accacttggg tcatttctac cttttggcca ttgcaataat
     6241 gttgctgtca acaaggatgt acaaatatct gttcgagtcc ctgctcttaa ttgttttggg
     6301 tatacaccca acctacatta atctttaatc acttaagatg ttggggtttg tgctgggagg
     6361 taaccaagtg actctggagc agcctcaccc tagctacaga gggaaactga ctcttccctc
     6421 tctgcccaat cttttgccgc ctgtcaggtt cccaagctgt ccatacctta ctgtgtgaag
     6481 cgctttgggt ataaactccc aaatagaggc tcagcccctt ttcctttccc aagtccatcc
     6541 tgcaggttat agctcttcag aggaatctct tgaattctgg cctgggatct gggattaaag
     6601 tagaacccag ggtgtccctt ggggccttag gcatagcccg ttgtggtgga acctgccatc
     6661 agccccactg cactcagtgg tcccctggta cttcaggaca gtttctctct tctgcacatg
     6721 agaacttcaa gcagagagga ctgtcagttg ttgccttcac atcaccaatc gtttggccat
     6781 aatggccaag agccctcact gctcttgggt cttgcctttc cacatgttac tgaaagctcc
     6841 cgttggtgtc cctgtccact aactcacctg atgggacaga tgccactttc ctctgagtgt
     6901 ctgataaaac acagtctgtt gtggggagcc cacgctcaag ggttttcttt gggatcagag
     6961 tttcccggct attactccat gccctgcctg acatcactgt catgtatggt gctaatcacc
     7021 aaaggatgcc cttgtttgat tctggattct aggtgatagt ccttatcctc tgatggggga
     7081 agagtttggg tgggagagaa caaaccgaca agttggttcc agatgaggtg gctgggagcc
     7141 aaccctcagg tacttggctc tgagtaatgt gacggcccag gcaagagggc tcccactgcc
     7201 aatgaggaac aaaagctcaa ggtctagtag tgtattcagc acatgctagg aaacaagagt
     7261 tgggacgtgg acaaggagag gagcaaaggc caggggttaa ctgcttttgg tttttcctct
     7321 ataaaagtca gggactatga tttatatggg tgagaaggtt tggagtagga ctgaagctgc
     7381 catttacaaa ccccttgccc taatttattg tagttttggg tgtgtttctg aaagaacctt
     7441 ttgcaggtga tttttattca gtactcatta acttacagct tttatgaatt cacatcttct
     7501 acactattga aaaacataag tttgtattgt gttaagtgca gcatgagtta ttatatgaat
     7561 aaatcacatt aaatttgcca
//
