LOCUS       NM_006899               1542 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens isocitrate dehydrogenase (NAD(+)) 3 non-catalytic
            subunit beta (IDH3B), transcript variant 1, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_006899
VERSION     NM_006899.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Wang,X., Liu,Q., Yu,H.T., Xie,J.Z., Zhao,J.N., Fang,Z.T., Qu,M.,
            Zhang,Y., Yang,Y. and Wang,J.Z.
  TITLE     A positive feedback inhibition of isocitrate dehydrogenase 3beta on
            paired-box gene 6 promotes Alzheimer-like pathology
  JOURNAL   Signal Transduct Target Ther 9 (1), 105 (2024)
   PUBMED   38679634
  REMARK    GeneRIF: A positive feedback inhibition of isocitrate dehydrogenase
            3beta on paired-box gene 6 promotes Alzheimer-like pathology.
            Erratum:[Signal Transduct Target Ther. 2024 Jul 15;9(1):187. doi:
            10.1038/s41392-024-01904-2.. PMID: 39009590]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 1542)
  AUTHORS   Bizjak,T., Gorenjak,M., Potocnik,U. and But,I.
  TITLE     Polymorphism on chromosome 20p13 near the IDH3B gene is associated
            with uterine prolapse
  JOURNAL   Eur J Obstet Gynecol Reprod Biol 252, 155-159 (2020)
   PUBMED   32619879
  REMARK    GeneRIF: Polymorphism on chromosome 20p13 near the IDH3B gene is
            associated with uterine prolapse.
REFERENCE   4  (bases 1 to 1542)
  AUTHORS   Sun,P., Ma,T., Zhang,T., Zhu,H., Zhang,J., Liu,Y. and Ding,J.
  TITLE     Molecular basis for the function of the alphabeta heterodimer of
            human NAD-dependent isocitrate dehydrogenase
  JOURNAL   J Biol Chem 294 (44), 16214-16227 (2019)
   PUBMED   31515270
REFERENCE   5  (bases 1 to 1542)
  AUTHORS   Wu,Q., Zhang,W., Xue,L., Wang,Y., Fu,M., Ma,L., Song,Y. and
            Zhan,Q.M.
  TITLE     APC/C-CDH1-Regulated IDH3beta Coordinates with the Cell Cycle to
            Promote Cell Proliferation
  JOURNAL   Cancer Res 79 (13), 3281-3293 (2019)
   PUBMED   31053633
  REMARK    GeneRIF: High IDH3B expression is associated with esophageal
            squamous cell carcinoma.
REFERENCE   6  (bases 1 to 1542)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   7  (bases 1 to 1542)
  AUTHORS   Weiss,C., Zeng,Y., Huang,J., Sobocka,M.B. and Rushbrook,J.I.
  TITLE     Bovine NAD+-dependent isocitrate dehydrogenase: alternative
            splicing and tissue-dependent expression of subunit 1
  JOURNAL   Biochemistry 39 (7), 1807-1816 (2000)
   PUBMED   10677231
REFERENCE   8  (bases 1 to 1542)
  AUTHORS   Kim,Y.O., Koh,H.J., Kim,S.H., Jo,S.H., Huh,J.W., Jeong,K.S.,
            Lee,I.J., Song,B.J. and Huh,T.L.
  TITLE     Identification and functional characterization of a novel,
            tissue-specific NAD(+)-dependent isocitrate dehydrogenase beta
            subunit isoform
  JOURNAL   J Biol Chem 274 (52), 36866-36875 (1999)
   PUBMED   10601238
REFERENCE   9  (bases 1 to 1542)
  AUTHORS   Kim,Y.O., Park,S.H., Kang,Y.J., Koh,H.J., Kim,S.H., Park,S.Y.,
            Sohn,U. and Huh,T.L.
  TITLE     Assignment of mitochondrial NAD(+)-specific isocitrate
            dehydrogenase beta subunit gene (IDH3B) to human chromosome band
            20p13 by in situ hybridization and radiation hybrid mapping
  JOURNAL   Cytogenet Cell Genet 86 (3-4), 240-241 (1999)
   PUBMED   10575215
REFERENCE   10 (bases 1 to 1542)
  AUTHORS   Fahim,A.T., Daiger,S.P. and Weleber,R.G.
  TITLE     Nonsyndromic Retinitis Pigmentosa Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301590
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY131986.1 and BC001960.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_006899.4.
            
            Summary: Isocitrate dehydrogenases catalyze the oxidative
            decarboxylation of isocitrate to 2-oxoglutarate. These enzymes
            belong to two distinct subclasses, one of which utilizes NAD(+) as
            the electron acceptor and the other NADP(+). Five isocitrate
            dehydrogenases have been reported: three NAD(+)-dependent
            isocitrate dehydrogenases, which localize to the mitochondrial
            matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of
            which is mitochondrial and the other predominantly cytosolic.
            NAD(+)-dependent isocitrate dehydrogenases catalyze the
            allosterically regulated rate-limiting step of the tricarboxylic
            acid cycle. Each isozyme is a heterotetramer that is composed of
            two alpha subunits, one beta subunit, and one gamma subunit. The
            protein encoded by this gene is the beta subunit of one isozyme of
            NAD(+)-dependent isocitrate dehydrogenase. Multiple alternatively
            spliced transcript variants encoding different isoforms have been
            described for this gene. [provided by RefSeq, Sep 2016].
            
            Transcript Variant: This variant (1) represents the predominate
            transcript and encodes isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189652.130447.1,
                                           SRR3476690.285142.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000380843.9/
                                                  ENSP00000370223.4
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                HY131986.1         9-27
            20-1542             BC001960.1         10-1532
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p13"
     gene            1..1542
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /note="isocitrate dehydrogenase (NAD(+)) 3 non-catalytic
                     subunit beta"
                     /db_xref="GeneID:3420"
                     /db_xref="HGNC:HGNC:5385"
                     /db_xref="MIM:604526"
     exon            1..64
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1186
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /EC_number="1.1.1.41"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; isocitrate dehydrogenase [NAD] subunit beta,
                     mitochondrial; NAD(+)-specific ICDH subunit beta;
                     isocitrate dehydrogenase (NAD(+)) 3 beta; isocitrate
                     dehydrogenase 3 (NAD+) beta; isocitric dehydrogenase
                     subunit beta; isocitrate dehydrogenase 3 (NAD(+)) beta"
                     /codon_start=1
                     /product="isocitrate dehydrogenase [NAD] subunit beta,
                     mitochondrial isoform a precursor"
                     /protein_id="NP_008830.2"
                     /db_xref="CCDS:CCDS13032.1"
                     /db_xref="GeneID:3420"
                     /db_xref="HGNC:HGNC:5385"
                     /db_xref="MIM:604526"
                     /translation="MAALSGVRWLTRALVSAGNPGAWRGLSTSAAAHAASRSQAEDVR
                     VEGSFPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLS
                     SMKENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDL
                     VIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHK
                     ANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNI
                     IDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLR
                     HLNLEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHLQTKGS"
     exon            65..145
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /inference="alignment:Splign:2.1.0"
     exon            146..244
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /inference="alignment:Splign:2.1.0"
     exon            245..365
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /inference="alignment:Splign:2.1.0"
     exon            366..426
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /inference="alignment:Splign:2.1.0"
     exon            427..559
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /inference="alignment:Splign:2.1.0"
     exon            560..693
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /inference="alignment:Splign:2.1.0"
     exon            694..796
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /inference="alignment:Splign:2.1.0"
     exon            797..943
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1038
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1099
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1542
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1524..1529
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /note="hexamer: AATAAA"
     polyA_site      1542
                     /gene="IDH3B"
                     /gene_synonym="RP46"
                     /note="major polyA site"
ORIGIN      
        1 acttcccacg cgacttcctg cgggaaacat ggcggcattg agcggagtcc gctggctgac
       61 ccgagcgctg gtctccgccg ggaaccctgg ggcatggaga ggtctgagta cctcggccgc
      121 ggcgcacgct gcatcgcgga gccaggccga ggacgtgagg gtggagggct cctttcccgt
      181 gaccatgctt ccgggagacg gtgtggggcc tgagctgatg cacgccgtca aggaggtgtt
      241 caaggctgcc gctgtcccag tggagttcca ggagcaccac ctgagtgagg tgcagaatat
      301 ggcatctgag gagaagctgg agcaggtgct gagttccatg aaggagaaca aagtggccat
      361 cattggaaag attcataccc cgatggagta taagggggag ctagcctcct atgatatgcg
      421 gctgaggcgt aagttggact tatttgccaa cgtagtccat gtgaagtcac ttcctgggta
      481 tatgactcgg cacaacaatc tagacctggt gatcattcga gagcagacag aaggggagta
      541 cagctctctg gaacatgaga gtgcaagggg tgtgattgag tgtttgaaga ttgtcacacg
      601 agccaagtct cagcggattg caaagttcgc ctttgactat gccaccaaga aggggcgggg
      661 caaggtcact gctgtccaca aggccaacat catgaaactt ggggatgggt tgttcctgca
      721 gtgctgtgag gaagttgctg aactgtaccc caaaatcaaa tttgagacaa tgatcataga
      781 caactgctgc atgcagctgg tgcagaatcc ttaccagttt gatgtgcttg tgatgcccaa
      841 tctctatggg aacattattg acaatctggc tgctggcctg gttgggggag ctggtgtggt
      901 ccctggtgag agctatagtg cagaatacgc agtctttgag acgggtgccc ggcacccatt
      961 tgcccaggca gtgggcagga atatagccaa tcccacggcc atgctgctgt cggcttccaa
     1021 catgctgcgg catcttaatc ttgagtatca ctccagcatg atcgcagatg cggtgaagaa
     1081 ggtgatcaaa gttggcaagg tgcggactcg agacatgggc ggctacagca ccacaaccga
     1141 cttcatcaag tctgtcatcg gtcacctgca gactaaaggg agctagagcc ctttatttct
     1201 tccaaccttg caaggaccac actccccata cccttcagtg cagtgtacca gggaagagac
     1261 cttgtgcctc taagcagtgg accatggtca ccttgctggg tagagcctag gttgtccttg
     1321 ggccggcttc cttaggggac agactgttgg gtggtgatgg ggattgttag gatggagccc
     1381 aggccacatg gatgatgatg attctccccc acaggttcga acctctgaca tgggtggcta
     1441 tgctacttgc catgacttca ctgaggctgt cattgctgcc ttgccccacc cataggccct
     1501 gtccataccc atgtaaggtg ttcaataaag aacatgaacc aa
//
