LOCUS       NM_005530               4083 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens isocitrate dehydrogenase (NAD(+)) 3 catalytic subunit
            alpha (IDH3A), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_005530
VERSION     NM_005530.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Song,X., Wu,X., Zhang,Z., Cui,Z., Zheng,Y. and Sun,J.
  TITLE     Subcellular Proteome Analysis Reveals Apoptotic Vulnerability of
            T-Cell Acute Lymphoblastic Leukemia
  JOURNAL   Biomed Res Int 2022, 5504475 (2022)
   PUBMED   35463978
  REMARK    GeneRIF: Subcellular Proteome Analysis Reveals Apoptotic
            Vulnerability of T-Cell Acute Lymphoblastic Leukemia.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 4083)
  AUTHORS   Osada,Y., Saito,R., Miyata,S., Shoji,T., Shibahara,I., Kanamori,M.,
            Sonoda,Y., Kumabe,T., Watanabe,M. and Tominaga,T.
  TITLE     Association between IDH mutational status and tumor-associated
            epilepsy or venous thromboembolism in patients with grade II and
            III astrocytoma
  JOURNAL   Brain Tumor Pathol 38 (3), 218-227 (2021)
   PUBMED   34269949
  REMARK    GeneRIF: Association between IDH mutational status and
            tumor-associated epilepsy or venous thromboembolism in patients
            with grade II and III astrocytoma.
REFERENCE   4  (bases 1 to 4083)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4083)
  AUTHORS   Sun,P., Liu,Y., Ma,T. and Ding,J.
  TITLE     Structure and allosteric regulation of human NAD-dependent
            isocitrate dehydrogenase
  JOURNAL   Cell Discov 6 (1), 94 (2020)
   PUBMED   33349631
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4083)
  AUTHORS   Weiss,C., Zeng,Y., Huang,J., Sobocka,M.B. and Rushbrook,J.I.
  TITLE     Bovine NAD+-dependent isocitrate dehydrogenase: alternative
            splicing and tissue-dependent expression of subunit 1
  JOURNAL   Biochemistry 39 (7), 1807-1816 (2000)
   PUBMED   10677231
REFERENCE   7  (bases 1 to 4083)
  AUTHORS   Kim,Y.O., Koh,H.J., Kim,S.H., Jo,S.H., Huh,J.W., Jeong,K.S.,
            Lee,I.J., Song,B.J. and Huh,T.L.
  TITLE     Identification and functional characterization of a novel,
            tissue-specific NAD(+)-dependent isocitrate dehydrogenase beta
            subunit isoform
  JOURNAL   J Biol Chem 274 (52), 36866-36875 (1999)
   PUBMED   10601238
REFERENCE   8  (bases 1 to 4083)
  AUTHORS   Huh,T.L., Kim,Y.O., Oh,I.U., Song,B.J. and Inazawa,J.
  TITLE     Assignment of the human mitochondrial NAD+ -specific isocitrate
            dehydrogenase alpha subunit (IDH3A) gene to 15q25.1-->q25.2by in
            situ hybridization
  JOURNAL   Genomics 32 (2), 295-296 (1996)
   PUBMED   8833160
  REMARK    Erratum:[Genomics 1996 Jul 1;35(1):274]
REFERENCE   9  (bases 1 to 4083)
  AUTHORS   Kim,Y.O., Oh,I.U., Park,H.S., Jeng,J., Song,B.J. and Huh,T.L.
  TITLE     Characterization of a cDNA clone for human NAD(+)-specific
            isocitrate dehydrogenase alpha-subunit and structural comparison
            with its isoenzymes from different species
  JOURNAL   Biochem J 308 (Pt 1) (Pt 1), 63-68 (1995)
   PUBMED   7755589
REFERENCE   10 (bases 1 to 4083)
  AUTHORS   Cohen,P.F. and Colman,R.F.
  TITLE     Diphosphopyridine nucleotide dependent isocitrate dehydrogenase
            from pig heart. Charactgerization of the active substrate and modes
            of regulation
  JOURNAL   Biochemistry 11 (8), 1501-1508 (1972)
   PUBMED   4336621
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC090260.13.
            
            On Nov 26, 2018 this sequence version replaced NM_005530.2.
            
            Summary: Isocitrate dehydrogenases catalyze the oxidative
            decarboxylation of isocitrate to 2-oxoglutarate. These enzymes
            belong to two distinct subclasses, one of which utilizes NAD(+) as
            the electron acceptor and the other NADP(+). Five isocitrate
            dehydrogenases have been reported: three NAD(+)-dependent
            isocitrate dehydrogenases, which localize to the mitochondrial
            matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of
            which is mitochondrial and the other predominantly cytosolic.
            NAD(+)-dependent isocitrate dehydrogenases catalyze the
            allosterically regulated rate-limiting step of the tricarboxylic
            acid cycle. Each isozyme is a heterotetramer that is composed of
            two alpha subunits, one beta subunit, and one gamma subunit. The
            protein encoded by this gene is the alpha subunit of one isozyme of
            NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.12981.1,
                                           SRR1660805.227679.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000299518.7/
                                                  ENSP00000299518.2
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-69                AC090260.13        50658-50726         c
            70-132              AC090260.13        44813-44875         c
            133-216             AC090260.13        42457-42540         c
            217-331             AC090260.13        39882-39996         c
            332-519             AC090260.13        38320-38507         c
            520-653             AC090260.13        37721-37854         c
            654-756             AC090260.13        36479-36581         c
            757-821             AC090260.13        36308-36372         c
            822-906             AC090260.13        35012-35096         c
            907-1059            AC090260.13        33786-33938         c
            1060-4083           AC090260.13        28143-31166         c
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q25.1"
     gene            1..4083
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /note="isocitrate dehydrogenase (NAD(+)) 3 catalytic
                     subunit alpha"
                     /db_xref="GeneID:3419"
                     /db_xref="HGNC:HGNC:5384"
                     /db_xref="MIM:601149"
     exon            1..69
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..1143
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /EC_number="1.1.1.41"
                     /note="H-IDH alpha; isocitrate dehydrogenase [NAD] subunit
                     alpha, mitochondrial; NAD+-specific ICDH; NAD(+)-specific
                     ICDH subunit alpha; isocitrate dehydrogenase 3 (NAD+)
                     alpha; isocitric dehydrogenase subunit alpha;
                     NAD(H)-specific isocitrate dehydrogenase alpha subunit;
                     isocitrate dehydrogenase (NAD+) alpha chain; isocitrate
                     dehydrogenase 3 (NAD(+)) alpha; isocitrate dehydrogenase
                     (NAD(+)) 3 alpha"
                     /codon_start=1
                     /product="isocitrate dehydrogenase [NAD] subunit alpha,
                     mitochondrial precursor"
                     /protein_id="NP_005521.1"
                     /db_xref="CCDS:CCDS10297.1"
                     /db_xref="GeneID:3419"
                     /db_xref="HGNC:HGNC:5384"
                     /db_xref="MIM:601149"
                     /translation="MAGPAWISKVSRLLGAFHNPKQVTRGFTGGVQTVTLIPGDGIGP
                     EISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLKTP
                     IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVI
                     VDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREV
                     AESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSG
                     NIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFAT
                     IKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD"
     exon            70..132
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /inference="alignment:Splign:2.1.0"
     exon            133..216
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /inference="alignment:Splign:2.1.0"
     exon            217..331
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /inference="alignment:Splign:2.1.0"
     exon            332..519
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /inference="alignment:Splign:2.1.0"
     exon            520..653
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /inference="alignment:Splign:2.1.0"
     exon            654..756
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /inference="alignment:Splign:2.1.0"
     exon            757..821
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /inference="alignment:Splign:2.1.0"
     exon            822..906
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /inference="alignment:Splign:2.1.0"
     exon            907..1059
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..4083
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1723..1728
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /note="hexamer: AATAAA"
     polyA_site      1748
                     /gene="IDH3A"
                     /gene_synonym="RP90"
     regulatory      2660..2665
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /note="hexamer: AATAAA"
     polyA_site      2680
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /note="major polyA site"
     regulatory      4058..4063
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IDH3A"
                     /gene_synonym="RP90"
                     /note="hexamer: AATAAA"
     polyA_site      4083
                     /gene="IDH3A"
                     /gene_synonym="RP90"
ORIGIN      
        1 actgccgctg cggctgttgc tgcggagcca ggaggggaag cgatggctgg gcccgcgtgg
       61 atctctaagg tctctcggct gctgggggca ttccacaacc caaaacaggt gaccagaggt
      121 tttactggtg gtgttcagac agtaacttta attccaggag atggtattgg cccagaaatt
      181 tcagctgcag ttatgaagat ttttgatgct gccaaagcac ctattcagtg ggaggagcgg
      241 aacgtcactg ccattcaagg acctggagga aagtggatga tcccttcaga ggctaaagag
      301 tccatggata agaacaagat gggcttgaaa ggccctttga agaccccaat agcagccggt
      361 cacccatcta tgaatttact gctgcgcaaa acatttgacc tttacgcgaa tgtccgacca
      421 tgtgtctcta tcgaaggcta taaaacccct tacaccgatg taaatattgt gaccattcga
      481 gagaacacag aaggagaata cagtggaatt gagcatgtga ttgttgatgg agtcgtgcag
      541 agtatcaagc tcatcaccga gggggcgagc aagcgcattg ctgagtttgc ctttgagtat
      601 gcccggaaca accaccggag caacgtcacg gcggtgcaca aagccaacat catgcggatg
      661 tcagatgggc tttttctaca aaaatgcagg gaagttgcag aaagctgtaa agatattaaa
      721 tttaatgaga tgtaccttga tacagtatgt ttgaatatgg tacaagatcc ttcccaattt
      781 gatgttcttg ttatgccaaa tttgtatgga gacatcctta gtgacttgtg tgcaggattg
      841 atcggaggtc tcggtgtgac accaagtggc aacattggag ccaatggggt tgcaattttt
      901 gagtcggttc atgggacggc tccagacatt gcaggcaagg acatggcgaa tcccacagcc
      961 ctcctgctca gtgccgtgat gatgctgcgc cacatgggac tttttgacca tgctgcaaga
     1021 attgaggctg cgtgttttgc tacaattaag gacggaaaga gcttgacaaa agatttggga
     1081 ggcaatgcaa aatgctcaga cttcacagag gaaatctgtc gccgagtaaa agatttagat
     1141 taacacttct acaactggca tttacatcag tcactctaaa tggacaccac atgaacctct
     1201 gtttagaata cctacgtatg tatgcattgg tttgcttgtt tcttgacagt acatttttag
     1261 atctggcctt ttcttaacaa aatctgtgca aaagatgcag gtggatgtcc ctaggtctgt
     1321 tttcaaagaa ctttttccaa gtgcttgttt tatttattaa gtgtctacct ggtaaatgtt
     1381 ttttttgtaa actctgagtg gactgtatca tttgctattc taaaccattt tacacttaag
     1441 ttaaaatagt ttctcttcag ctgtaaataa caggatacag aattaacaag agaaaatgtc
     1501 taacttttta agaaaaacct tattttcttc ggtttttgaa aaacataatg gaaataaaac
     1561 aggatattga cataatagca caaaatgaca ctcttctaaa actaaatggg cacaagagaa
     1621 ttttcctggg aaagttcaca tcaaaaagag tgaatgtggt atatttctaa atgatatgga
     1681 aaatagagac agatttgtcc tttacagaaa ttactgagtg tgaataaaaa cttcagatcc
     1741 aagaaatata taatgagaga tataattttt gttaataaga caaaggtaat atattggata
     1801 caaagacaca aatgtattgt gtgttcaatt attttgttgt cttgagattt aatattcttt
     1861 ccaagagctt ttaatgaagc agagagctag tacttcattt tcactggata cattttcagc
     1921 atcatgagtt gtcacagcct ctgagcccct gatctgaagc cagaagggct gagtgtattg
     1981 taaacttatt cttgcatgtt gctgtctggg aatggaccac actacagcag gtagttctgg
     2041 gggcgatact gccgaaaggc ccgaacacat gtattttggc tgcaattgag gaacttggga
     2101 tgctattaat tttgtatttc agcaactgcc ccttctccta tcccaaagca ccaattactg
     2161 ccctctgcct cagcagtacc agtataagat gacattccaa agactggagg caactcagcc
     2221 tgagttaatt cacaaaatta tgccatgctg gggcttgagc ttgagcttgg gcttaggctt
     2281 gggctcagct tttgaccctc aggcatctcc tttcccttcc tgtcttcctc tcccttctcc
     2341 tctgctgcag catgattttc ttaatcttca gacactcact attttcatga acagttaccc
     2401 tctgtcccca caaccaaaga caactcatgg cctcctttgg cccttgtgta acattgcaaa
     2461 cctgtggctt tgcaaaatgt acccaggtca caaggggatt tttttttttt tagcaatgat
     2521 atccctgtct gggtcacttt ttaagcttgt aaccgccccc ccagacttat aatcttaaat
     2581 gtattttcct ttgtttaagc tgctgcttcc tctgtttcat tggattgtgc cagttatcag
     2641 tggctcttgg gttcaaagta ataaagaatt ccaaaactga gatgtttgct ttctctacct
     2701 gtttcctgtt cagatgcctt cggtcatttt ggctcagctt gctactgaga atggctgctt
     2761 cccgtattca gaatggaagt ggggtggtct ggtggcgaca ggctaacgta gagctggctg
     2821 cttttatgag aatgcgccac ttgaccccat cctgcacctt tgtttattcc agggcactaa
     2881 tggtcactcg catgcccgct gtgcacagtc ctgtgagtta aatgcccact actctctgca
     2941 ctggtcccac ctgctcaatt gacaaaaaaa atcaggtcct tgttgacagt tctgttaaaa
     3001 ggtttaatat tcaaattata cattaaatag aatcaaacag gcagcagcag ttttattaag
     3061 catcaagtca gttggcatgt gggtggcgga gtgcctggta atggaataat agggtcggcg
     3121 atgcccgcca agattccaga gtaagtcagg tcgctaggtg agttgtgact gattgctttt
     3181 cctgcagcgc acgtgagcag tatcagccat ctctctccta cccgctccac agcctactgc
     3241 tggctgtcct gtatgtgagc tagcagcttt ttaatctacc tggaactaag gccaaaaatt
     3301 atcagccctt tcgttctgga aggagagctg atctcatttg tcacctgaac aaaaagcaat
     3361 acttaaactg aattaaaact acccacacgt gaagcttctt ggaattgtca gctattgttg
     3421 gcgtaagacc tggtaaatgt agagccagtg ctgtgccctg acctggagat ctaacagact
     3481 tggcagaaat gcctgtgccc agactgaaga gacctggggc tcaggaagag gctcggaacg
     3541 cctgccctct attctataga aactgcaggc ataggccctg attacatttt ttgctcttgg
     3601 taaaaggagt caatgatacc tttgtacttc tccaaaactg tgagccgttt cagtttcatc
     3661 gtgggaccta aaagggaaat gagaagaaat gagtgaggcc caggctctga gaactctgag
     3721 aatgtgtgtg gtaaagactc acactcagtc ctatatataa ctcaggaatt aagccagata
     3781 atcaggaccg tcagtagcca gcctccaaga cagtgatcgc tcctggtatt catatggctt
     3841 gtgtgtagtc tcctgtgtta taccactggt tgtggccagt ggagtataga tgaagggttg
     3901 gtatgtcacc tctgagaata ggttataaaa gatcagtttc ttttatacac ataggaaagt
     3961 agtgtaagct acatctgcct tttccttgac ctgtacccag ttttgaccct cagtccattt
     4021 tctctgaaat ggatttattt gttccatctc ttgaatgaat aaattgttga agcaattaaa
     4081 aaa
//
