LOCUS       NM_012405               4795 bp    mRNA    linear   PRI 24-JUN-2024
DEFINITION  Homo sapiens isoprenylcysteine carboxyl methyltransferase (ICMT),
            mRNA.
ACCESSION   NM_012405
VERSION     NM_012405.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Borini Etichetti,C., Arel Zalazar,E., Di Benedetto,C.,
            Cocordano,N., Valente,S., Bicciato,S., Menacho-Marquez,M.,
            Larocca,M.C. and Girardini,J.
  TITLE     Isoprenylcysteine carboxyl methyltransferase (ICMT) promotes
            invadopodia formation and metastasis in cancer cells
  JOURNAL   Biochimie 222, 28-36 (2024)
   PUBMED   38301884
  REMARK    GeneRIF: Isoprenylcysteine carboxyl methyltransferase (ICMT)
            promotes invadopodia formation and metastasis in cancer cells.
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Sittewelle,M., Kappes,V., Zhou,C., Lecuyer,D. and Monsoro-Burq,A.H.
  TITLE     PFKFB4 interacts with ICMT and activates RAS/AKT
            signaling-dependent cell migration in melanoma
  JOURNAL   Life Sci Alliance 5 (12), e202201377 (2022)
   PUBMED   35914811
  REMARK    GeneRIF: PFKFB4 interacts with ICMT and activates RAS/AKT
            signaling-dependent cell migration in melanoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4795)
  AUTHORS   Li,Y., Gao,X., Yang,C., Yan,H. and Li,C.
  TITLE     CircRNA hsa_circ_0018289 exerts an oncogenic role in cervical
            cancer progression through miR-1294/ICMT axis
  JOURNAL   J Clin Lab Anal 36 (5), e24348 (2022)
   PUBMED   35312113
  REMARK    GeneRIF: CircRNA hsa_circ_0018289 exerts an oncogenic role in
            cervical cancer progression through miR-1294/ICMT axis.
REFERENCE   4  (bases 1 to 4795)
  AUTHORS   Ahearn,I.M., Court,H.R., Siddiqui,F., Abankwa,D. and Philips,M.R.
  TITLE     NRAS is unique among RAS proteins in requiring ICMT for trafficking
            to the plasma membrane
  JOURNAL   Life Sci Alliance 4 (5), e202000972 (2021)
   PUBMED   33579760
  REMARK    GeneRIF: NRAS is unique among RAS proteins in requiring ICMT for
            trafficking to the plasma membrane.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4795)
  AUTHORS   Chai,T.F., Manu,K.A., Casey,P.J. and Wang,M.
  TITLE     Isoprenylcysteine carboxylmethyltransferase is required for the
            impact of mutant KRAS on TAZ protein level and cancer cell
            self-renewal
  JOURNAL   Oncogene 39 (31), 5373-5389 (2020)
   PUBMED   32561852
  REMARK    GeneRIF: Isoprenylcysteine carboxylmethyltransferase is required
            for the impact of mutant KRAS on TAZ protein level and cancer cell
            self-renewal.
REFERENCE   6  (bases 1 to 4795)
  AUTHORS   Ahmad,M., Zhang,Y., Zhang,Y., Papharalambus,C. and Alexander,R.W.
  TITLE     Role of isoprenylcysteine carboxyl methyltransferase in tumor
            necrosis factor-alpha stimulation of expression of vascular cell
            adhesion molecule-1 in endothelial cells
  JOURNAL   Arterioscler Thromb Vasc Biol 22 (5), 759-764 (2002)
   PUBMED   12006387
  REMARK    GeneRIF: ICMTase is a critical component of the redox-sensitive
            VCAM-1-selective signaling pathway and appears to activate a
            discrete inflammatory signaling pathway, at least in part, through
            the methylation of Rac1 in endothelial cells.
REFERENCE   7  (bases 1 to 4795)
  AUTHORS   Van Dessel,G.A., De Busser,H.M. and Lagrou,A.R.
  TITLE     Prenylcysteine carboxymethyltransferase type III activity is
            decreased in retinoic acid-treated SH-SY5Y neuroblastoma cells
  JOURNAL   Int J Biochem Cell Biol 34 (5), 477-486 (2002)
   PUBMED   11906819
  REMARK    GeneRIF: modulation of pcCMT activity in retinoic acid (RA)-treated
            SH-SY5Y neuroblastoma cells
REFERENCE   8  (bases 1 to 4795)
  AUTHORS   Lin,X., Antalffy,B., Kang,D., Orr,H.T. and Zoghbi,H.Y.
  TITLE     Polyglutamine expansion down-regulates specific neuronal genes
            before pathologic changes in SCA1
  JOURNAL   Nat Neurosci 3 (2), 157-163 (2000)
   PUBMED   10649571
REFERENCE   9  (bases 1 to 4795)
  AUTHORS   Choy,E., Chiu,V.K., Silletti,J., Feoktistov,M., Morimoto,T.,
            Michaelson,D., Ivanov,I.E. and Philips,M.R.
  TITLE     Endomembrane trafficking of ras: the CAAX motif targets proteins to
            the ER and Golgi
  JOURNAL   Cell 98 (1), 69-80 (1999)
   PUBMED   10412982
REFERENCE   10 (bases 1 to 4795)
  AUTHORS   Dai,Q., Choy,E., Chiu,V., Romano,J., Slivka,S.R., Steitz,S.A.,
            Michaelis,S. and Philips,M.R.
  TITLE     Mammalian prenylcysteine carboxyl methyltransferase is in the
            endoplasmic reticulum
  JOURNAL   J Biol Chem 273 (24), 15030-15034 (1998)
   PUBMED   9614111
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CN343480.1, BC028168.1 and
            AL031847.17.
            
            On Nov 23, 2018 this sequence version replaced NM_012405.3.
            
            Summary: This gene encodes the third of three enzymes that
            posttranslationally modify isoprenylated C-terminal cysteine
            residues in certain proteins and target those proteins to the cell
            membrane. This enzyme localizes to the endoplasmic reticulum.
            Alternative splicing may result in other transcript variants, but
            the biological validity of those transcripts has not been
            determined. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.40820.1, AF064084.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467144 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000343813.10/ ENSP00000343552.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                CN343480.1         44-67
            25-2497             BC028168.1         1-2473
            2498-4795           AL031847.17        86373-88670
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.31"
     gene            1..4795
                     /gene="ICMT"
                     /gene_synonym="HSTE14; MST098; MSTP098; PCCMT; PCMT; PPMT"
                     /note="isoprenylcysteine carboxyl methyltransferase"
                     /db_xref="GeneID:23463"
                     /db_xref="HGNC:HGNC:5350"
                     /db_xref="MIM:605851"
     exon            1..248
                     /gene="ICMT"
                     /gene_synonym="HSTE14; MST098; MSTP098; PCCMT; PCMT; PPMT"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..908
                     /gene="ICMT"
                     /gene_synonym="HSTE14; MST098; MSTP098; PCCMT; PCMT; PPMT"
                     /EC_number="2.1.1.100"
                     /note="prenylcysteine carboxyl methyltransferase;
                     prenylated protein carboxyl methyltransferase"
                     /codon_start=1
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="NP_036537.1"
                     /db_xref="CCDS:CCDS61.1"
                     /db_xref="GeneID:23463"
                     /db_xref="HGNC:HGNC:5350"
                     /db_xref="MIM:605851"
                     /translation="MAGCAARAPPGSEARLSLATFLLGASVLALPLLTRAGLQGRTGL
                     ALYVAGLNALLLLLYRPPRYQIAIRACFLGFVFGCGTLLSFSQSSWSHFGWYMCSLSL
                     FHYSEYLVTAVNNPKSLSLDSFLLNHSLEYTVAALSSWLEFTLENIFWPELKQITWLS
                     VTGLLMVVFGECLRKAAMFTAGSNFNHVVQNEKSDTHTLVTSGVYAWFRHPSYVGWFY
                     WSIGTQVMLCNPICGVSYALTVWRFFRDRTEEEEISLIHFFGEEYLEYKKRVPTGLPF
                     IKGVKVDL"
     exon            249..337
                     /gene="ICMT"
                     /gene_synonym="HSTE14; MST098; MSTP098; PCCMT; PCMT; PPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            338..507
                     /gene="ICMT"
                     /gene_synonym="HSTE14; MST098; MSTP098; PCCMT; PCMT; PPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            508..725
                     /gene="ICMT"
                     /gene_synonym="HSTE14; MST098; MSTP098; PCCMT; PCMT; PPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            726..4795
                     /gene="ICMT"
                     /gene_synonym="HSTE14; MST098; MSTP098; PCCMT; PCMT; PPMT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4769..4774
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ICMT"
                     /gene_synonym="HSTE14; MST098; MSTP098; PCCMT; PCMT; PPMT"
                     /note="hexamer: AATAAA"
     polyA_site      4795
                     /gene="ICMT"
                     /gene_synonym="HSTE14; MST098; MSTP098; PCCMT; PCMT; PPMT"
                     /note="major polyA site"
ORIGIN      
        1 gtttctccgg gctacagccg gcgccgccgc ccgctagtcc gccgcccggc gccatggcgg
       61 gctgcgcggc gcgggctccg ccgggctctg aggcgcgtct cagcctcgcc accttcctgc
      121 tgggcgcctc ggtgctcgcg ctgccgctgc tcacgcgcgc cggcctgcag ggccgcaccg
      181 ggctggcgct ctacgtggcc gggctcaacg cgctgctgct gctgctctat cggccgcctc
      241 gctaccagat agccatccga gcttgtttcc tggggtttgt gttcggctgc ggcacgctgc
      301 taagttttag ccagtcttct tggagtcact ttggctggta catgtgctcc ctgtcattgt
      361 tccactattc tgaatacttg gtgacagcag tcaataatcc caaaagtctg tccttggatt
      421 cctttctcct gaatcacagc ctggagtata cagtagctgc tctttcttct tggttagagt
      481 tcacacttga aaatatcttt tggccagaac tgaagcagat tacctggctc agtgtcacag
      541 ggctgctgat ggtggtcttc ggagaatgtc tgaggaaggc ggccatgttt acagctggct
      601 ccaatttcaa ccacgtggta cagaatgaaa aatcagatac acatactctg gtgaccagtg
      661 gagtgtacgc ttggtttcgg catccttctt acgtcgggtg gttttactgg agtattggaa
      721 ctcaggtgat gctgtgtaac cccatctgcg gcgtcagcta tgccctgaca gtgtggcgat
      781 tcttccgcga tcgaacagaa gaagaagaaa tctcactaat tcactttttt ggagaggagt
      841 acctggagta taagaagagg gtgcccacgg gcctgccttt cataaagggg gtcaaggtgg
      901 acctgtgacg ggcagtggcc ccggtgacct tggggcctcc gaccctgtgc agcctgggac
      961 aaaactgttt ccggttggcc gctgccacat ggattttctt aatcgtttta tgtcattagt
     1021 cactcttctg gaatgtcact caagaccaag cggtcagaag gcctgaggac ccaaggcccc
     1081 actggagcag tctgtcctta tgccgaatca aggcggaaca tgggtgaaag acgagtaagg
     1141 ggcaaatcac agcaatattc cacagcgccc tccagagtta cctggggagg accgaggcca
     1201 cacgccactg cccccgaggc cagagtgtaa gtaaaggata accaggactc gctgggagag
     1261 atggactctg tcctcagcaa cactccacag cagaaagggg tagcaggtac cccttcttat
     1321 cagcggtaaa aatgcattta caacctttca tttaaccgaa aaacacagac cgctttaacc
     1381 tctttatttc tgtcccccac tgcatgaaca tctatacaat tttaaaaata cttcctcata
     1441 ggatgctttg gcccttcatc tatttaatca tagctacata cctatttttt ataagtagca
     1501 gtacacattc aaaggggtat tcctagctca atgcttggtg ttctagttca acttttatcc
     1561 tgcagcaagt aagcctagat aactctacac gatttggctg agtggctttg tgtgaccgtg
     1621 gccccaggcc aaggggatca tggccctggc tggctttccc gggggtctca gctcctgttg
     1681 tcagtgatag gcggctcaaa ggagcatcag tttcttttga tccaagaagt gcttactgaa
     1741 tgcctgccct gtgcgtggcc ttaaacattg agaagtgctg ctctccgttt atttgggatt
     1801 tgattctcat tttaccatag cttatattct caatttcaat gccagtctca gaactcttgt
     1861 tttctgtgtt ctgttctcaa aattacattg tccctcatgt catttcaaac tgttttccaa
     1921 agggatttga gcatatacaa ctacaaatcc aagcagattg actctcaaaa ataatcttaa
     1981 atactgcaaa tagtcccaac taagattcag tcagtatgtt tgttttgcaa gtttgggaga
     2041 gtaagttggc tttgagtcac acatcgaagc tttaagaggt gagacgctgg cttcattctg
     2101 gactagacag gaacttggcc tcagcgtgag atcctgccat gcagtgttgc ggtggcactg
     2161 aagaagtgtg aatgtgaagg cggcgtcggc gcggggccag agcaccactc tgctgcccca
     2221 ccacgcggcc tgtgaggagc cactaaacct ttccgtgcct agacctcccc atctgtggaa
     2281 tggggtcaat accacctacc tcacaggggt gttgtgagga ctgagaagaa caatgtcaaa
     2341 tgtttttaat actcagatgt gggagcgaca tcaatgaaat ctgtactgta tgaaagctac
     2401 acaaaaatgg gcagacattt ggttaattgt gccagatacc taaaatgtat gttcagaaaa
     2461 gcattttatc aactcagaaa tatgacttat ttctagattt catggcttaa tgaatttttt
     2521 cattgttata tataccaaag aggcttacgg gttcattgat tggtttgaaa accagacaga
     2581 cggccgggcg cggtggctca cgcctgtaat cccagcaatt tgggaggcca aggcgggtgg
     2641 atcatgaggt caggcgttcg agaccagcct ggccaacatg ataaaacccc gtctctacta
     2701 aaaatacaaa aaaatagctg ggcgtgctgg cgggtgcctg taatcccagc tactcaggag
     2761 gctgagacag gagaatcctt tgaacccagg aggcggaggt tgcagtgagt tgagatcggg
     2821 cctttgcact tcagtctggg caacagggcg agactccatc tcaaaaaaca acaacaacaa
     2881 caaaaaaaac cggacggact tgcccatcgg ccctcacgac acgcgtgcag tgggactcta
     2941 gccaaggcgg tggccgagcc atcattacaa tttttctgga gtaaaggatc cacggtggga
     3001 catcaactgg cacttactct gtttaggaac ttgagttgaa tcatttctaa acttgtcctt
     3061 tagaccacgc ctagggcagc aaattccact tcctagaact gcaaaccggg agaggatgta
     3121 gttagattct ggcatcctgc cccggctctt tgagggaaaa gttctttccc ctgctctaag
     3181 tagggcgggc ccagcctcca ttcccattgg gagccatgaa gttgtcttgt tgataggtct
     3241 agggcagctg ttggcagctg ctggccttgt ggatggtaag ctgactgtgt tggcatttaa
     3301 tgccatcctc catctgccag aggcccagga agcccacctt cacaccaggc ccaccagctg
     3361 gaaggagccc aggaggcagg tcttagggtt tcatggtgaa atgctgtctg acaccaggga
     3421 aaacttgacg tactctgctt tggagaggaa ttccaggggt agggatggtg ggagaacatt
     3481 cccaccctgt ttataatccc agagcagaag tcagcaatta cttagggata aaagatgaac
     3541 tccatttttt ttattttctt aagacagtct tgctctgtgg cccaggctgg aggctggagt
     3601 gcagtggcac catttcggct caccgcaacc tctgcctccc gggttcaagc gattctcctg
     3661 cctcagcctc ctgagtagct gggattacag gcttgggcca ccatgcccag ctaatttttt
     3721 tgtattttta gtagagacgg ggtttcacca tgatggccag gctggtctcg aactcctgac
     3781 ctcaagtgat ccacgcgcct cggcctccca aagtgctggg attacaggcg tgagccacca
     3841 cgcccagcca agatgaactc cttaaggaca ggatttggta agtgattgac ttctttttag
     3901 ttccatgatc ttgagattat ttttagcttt ataaatttag cagtggcagg gcccgtggag
     3961 aatcaggtta atgaggtaaa ggctttctgg gtatttgctg ccaaggccac atcaccaatt
     4021 ttctcgattt aaaaaactgt caagagattt atttttccat tgcaggtttt aaagtggaga
     4081 ttctgaagtg gaaaataggt actgtcagaa caaagctacc tggaaacagc atagagtgaa
     4141 gcctttcgtg agggcttgca ggccgctgct gagtggcagt ttacagaaga ggtcgcgggg
     4201 tgagcctctt agcaggacag aaaacaaggc agcagcgcac ctgccacccc ttcacgagct
     4261 gctccttgag cctaaaaagt aggctttatt catcccttct gttcatttac caacctgggg
     4321 gattgatacg accggggaaa atgttcctaa accaggaagc tgcgttagcc gatcaggctt
     4381 tgtaagatct cgccaacagc tagctgctta ggagtacccc cacgatacgc acagcacacc
     4441 actgtccctt cactgcactt tcttcctgcc ttaggtagtt gggcttgccc accctagttt
     4501 gcttttgtag tggtttggca aggttagaag gcctcggcct ctctgtcatg ctgggaagtg
     4561 cctactctct gggccactgc tgcagaggcc gtggcacttg tcatgggttt ggaagaccca
     4621 gccatctgca gcagaggcag cctatcccat tgcaaggaga ggaactgaac ggagtaatta
     4681 ttctactctt ctttttacat aaatgtttat ttaaatattc taaattggat tttctttcac
     4741 agatactgat tattctttcc agttcttaaa taaaactgca cttgatttca ctcaa
//
