LOCUS       NM_001010867            7828 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens iron-sulfur cluster assembly factor IBA57 (IBA57),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001010867 XM_114152
VERSION     NM_001010867.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Bargagna,B., Staderini,T., Lang,S.H., Banci,L. and Camponeschi,F.
  TITLE     Defects in the Maturation of Mitochondrial Iron-Sulfur Proteins:
            Biophysical Investigation of the MMDS3 Causing Gly104Cys Variant of
            IBA57
  JOURNAL   Int J Mol Sci 25 (19), 10466 (2024)
   PUBMED   39408793
  REMARK    GeneRIF: Defects in the Maturation of Mitochondrial Iron-Sulfur
            Proteins: Biophysical Investigation of the MMDS3 Causing Gly104Cys
            Variant of IBA57.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 7828)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 7828)
  AUTHORS   Nasta,V., Da Vela,S., Gourdoupis,S., Ciofi-Baffoni,S., Svergun,D.I.
            and Banci,L.
  TITLE     Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the
            mitochondrial iron-sulfur cluster assembly machinery
  JOURNAL   Sci Rep 9 (1), 18986 (2019)
   PUBMED   31831856
  REMARK    GeneRIF: Structural properties of [2Fe-2S] ISCA2-IBA57: a complex
            of the mitochondrial iron-sulfur cluster assembly machinery.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7828)
  AUTHORS   Hamanaka,K., Miyatake,S., Zerem,A., Lev,D., Blumkin,L., Yokochi,K.,
            Fujita,A., Imagawa,E., Iwama,K., Nakashima,M., Mitsuhashi,S.,
            Mizuguchi,T., Takata,A., Miyake,N., Saitsu,H., van der Knaap,M.S.,
            Lerman-Sagie,T. and Matsumoto,N.
  TITLE     Expanding the phenotype of IBA57 mutations: related leukodystrophy
            can remain asymptomatic
  JOURNAL   J Hum Genet 63 (12), 1223-1229 (2018)
   PUBMED   30258207
  REMARK    GeneRIF: a patient with infantile-onset optic atrophy and
            asymptomatic white matter involvement, thus broadening the
            phenotypic spectrum of biallelic IBA57 mutations.
REFERENCE   6  (bases 1 to 7828)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   7  (bases 1 to 7828)
  AUTHORS   Sheftel,A.D., Wilbrecht,C., Stehling,O., Niggemeyer,B.,
            Elsasser,H.P., Muhlenhoff,U. and Lill,R.
  TITLE     The human mitochondrial ISCA1, ISCA2, and IBA57 proteins are
            required for [4Fe-4S] protein maturation
  JOURNAL   Mol Biol Cell 23 (7), 1157-1166 (2012)
   PUBMED   22323289
  REMARK    GeneRIF: ISCA1, ISCA2, and IBA57 were depleted by RNA interference.
            Depleted cells contained massively swollen and enlarged
            mitochondria that were virtually devoid of cristae membranes,
            demonstrating the importance of these proteins for mitochondrial
            biogenesis.
REFERENCE   8  (bases 1 to 7828)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 7828)
  AUTHORS   Nilsson,R., Schultz,I.J., Pierce,E.L., Soltis,K.A.,
            Naranuntarat,A., Ward,D.M., Baughman,J.M., Paradkar,P.N.,
            Kingsley,P.D., Culotta,V.C., Kaplan,J., Palis,J., Paw,B.H. and
            Mootha,V.K.
  TITLE     Discovery of genes essential for heme biosynthesis through
            large-scale gene expression analysis
  JOURNAL   Cell Metab 10 (2), 119-130 (2009)
   PUBMED   19656490
  REMARK    GeneRIF: Required for functional heme biosynthesis in erythroid
            cells.
REFERENCE   10 (bases 1 to 7828)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL359510.24, HY081013.1 and
            BI820993.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001010867.3.
            
            Summary: The protein encoded by this gene localizes to the
            mitochondrion and is part of the iron-sulfur cluster assembly
            pathway. The encoded protein functions late in the biosynthesis of
            mitochondrial 4Fe-4S proteins. Defects in this gene have been
            associated with autosomal recessive spastic paraplegia-74 and with
            multiple mitochondrial dysfunctions syndrome-3. Two transcript
            variants encoding different isoforms have been found for this gene.
            The smaller isoform is not likely to be localized to the
            mitochondrion since it lacks the amino-terminal transit peptide.
            [provided by RefSeq, Jul 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.402822.1,
                                           SRR1163655.527695.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000366711.4/
                                                  ENSP00000355672.3
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AL359510.24        46876-46879
            5-484               HY081013.1         1-480
            485-946             BI820993.1         221-682
            947-7828            AL359510.24        56448-63329
FEATURES             Location/Qualifiers
     source          1..7828
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q42.13"
     gene            1..7828
                     /gene="IBA57"
                     /gene_synonym="C1orf69; MMDS3; SPG74"
                     /note="iron-sulfur cluster assembly factor IBA57"
                     /db_xref="GeneID:200205"
                     /db_xref="HGNC:HGNC:27302"
                     /db_xref="MIM:615316"
     exon            1..354
                     /gene="IBA57"
                     /gene_synonym="C1orf69; MMDS3; SPG74"
                     /inference="alignment:Splign:2.1.0"
     CDS             14..1084
                     /gene="IBA57"
                     /gene_synonym="C1orf69; MMDS3; SPG74"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; iron-sulfur cluster assembly factor for biotin
                     synthase- and aconitase-like mitochondrial proteins, with
                     a mass of 57kDa; putative transferase C1orf69,
                     mitochondrial; putative transferase CAF17, mitochondrial;
                     IBA57, iron-sulfur cluster assembly homolog; IBA57
                     homolog, iron-sulfur cluster assembly"
                     /codon_start=1
                     /product="putative transferase CAF17, mitochondrial
                     isoform 1 precursor"
                     /protein_id="NP_001010867.1"
                     /db_xref="CCDS:CCDS31046.1"
                     /db_xref="GeneID:200205"
                     /db_xref="HGNC:HGNC:27302"
                     /db_xref="MIM:615316"
                     /translation="MATAALLRGATPGRGGPVWRWRLRAAPRCRLAHSSCSPGGDPTA
                     GAAWACFRLDGRTLLRVRGPDAAPFLLGLLTNELPLPSPAAAGAPPAARAGYAHFLNV
                     QGRTLYDVILYGLQEHSEVSGFLLECDSSVQGALQKHLALYRIRRKVTVEPHPELRVW
                     AVLPSSPEACGAASLQERAGAAAILIRDPRTARMGWRLLTQDEGPALVPGGRLGDLWD
                     YHQHRYLQGVPEGVRDLPPGVALPLESNLAFMNGVSFTKGCYIGQELTARTHHMGVIR
                     KRLFPVRFLDPLPTSGITPGATVLTASGQTVGKFRAGQGNVGLALLWSEKIKGPLHIR
                     ASEGAQVALAASVPDWWPTVSK"
     exon            355..692
                     /gene="IBA57"
                     /gene_synonym="C1orf69; MMDS3; SPG74"
                     /inference="alignment:Splign:2.1.0"
     exon            693..7828
                     /gene="IBA57"
                     /gene_synonym="C1orf69; MMDS3; SPG74"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1932..1937
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IBA57"
                     /gene_synonym="C1orf69; MMDS3; SPG74"
                     /note="hexamer: ATTAAA"
     polyA_site      1953
                     /gene="IBA57"
                     /gene_synonym="C1orf69; MMDS3; SPG74"
                     /note="major polyA site"
     regulatory      4921..4926
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IBA57"
                     /gene_synonym="C1orf69; MMDS3; SPG74"
                     /note="hexamer: AATAAA"
     polyA_site      4941
                     /gene="IBA57"
                     /gene_synonym="C1orf69; MMDS3; SPG74"
     regulatory      7806..7811
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IBA57"
                     /gene_synonym="C1orf69; MMDS3; SPG74"
                     /note="hexamer: AATAAA"
     polyA_site      7828
                     /gene="IBA57"
                     /gene_synonym="C1orf69; MMDS3; SPG74"
ORIGIN      
        1 actcttgtcc aagatggcga ccgcggcgct gcttcgaggc gccactccgg ggcgcggcgg
       61 cccggtctgg cgctggcggc tgcgcgcggc cccaaggtgc cgcctggccc acagctcctg
      121 cagtcctggt ggcgacccaa cggccggagc ggcctgggcc tgcttccggc tggacgggcg
      181 caccctgctg cgcgtgcgtg gccccgacgc ggcgcccttc ctgctagggc tgctgaccaa
      241 tgaactgccg cttccgagtc ctgcggccgc gggggccccg cctgctgcgc gcgcgggcta
      301 cgcccacttc ctgaacgtgc agggccggac gctctatgac gtcatcttgt acgggctcca
      361 ggaacactcg gaggtgtctg gcttccttct ggagtgtgac agctcggtgc agggcgcgct
      421 gcagaagcac ctcgcgctat acaggatccg gcggaaggtc acggtggagc cgcacccgga
      481 gctgcgagtg tgggcggtgt tgcccagttc ccctgaggcc tgcggggctg catcgctgca
      541 ggagagggca ggggctgccg ccatcctcat ccgcgacccg cgaacagcac gcatggggtg
      601 gcggctcctc acccaggatg aaggcccagc cctggtgccc gggggccggc tcggggactt
      661 gtgggattat caccagcacc gatacctgca aggcgttcct gagggggtcc gagacttgcc
      721 tcctggggtg gccctgcccc tggagtccaa cctggccttc atgaacggcg tgagcttcac
      781 caaaggctgc tacattggcc aggagctgac ggcccgcacc caccacatgg gcgtcatccg
      841 caagcgcctc ttccctgtcc ggttcttgga cccccttccc accagtggca tcacccctgg
      901 tgccacggtg ctgactgcct caggacagac tgtgggcaag ttcagggctg gccagggcaa
      961 cgtggggctg gccctgctgt ggtcagagaa gatcaagggt cctctgcaca tcagagcctc
     1021 tgagggtgcc caggtggcct tagccgcatc tgtgccagac tggtggccta cagtctccaa
     1081 gtagtccgaa gccttggctg gcgcaggctg atggggaggc tggggcctgg ggcctttggc
     1141 ctctgtccag ggtcttcccg tcccatctgt ctgctgcgcc tactgggtgg gagccctggt
     1201 ttccatctgg gaaagtcccc cttgtaggtg ccctgcctgg ccccacccat gctcaggggc
     1261 cccaggcacg tgggttgttt tctccctgga cttcctgtgg ccccagagga gcccaccgtg
     1321 tgagtgctgg gctccagatg gcgccgggtc ccaatcgtgg ctccacacag ggttgtctgg
     1381 gaggacggga cggtgtctct ggccagcact ggcagcttcc gggccttgta ccccactctg
     1441 cctggcatga gcctcatggg gggttggtcc ctgtgcctgg aagcggggtg gtgtccaggg
     1501 tctgcaggct ggggctcggg ctttgggagt tgtttcactg tgcttgcttc cagcatgtct
     1561 cagggtgggg ctatgcgtgg tctagagggg tctggggcat tggatcaggt ggcatgtggg
     1621 tgtctacaca tcgagaagca cacagggatg agccactagg tatcccatgc tggaggcttg
     1681 tggaaggctg tgcccatctc ccatgtttcc ccacctctgt tcctcctcag agccctccag
     1741 gagcgtgggg tgggggccag cgcagggctc aggagccagc agggcagagt tctgcctgtg
     1801 tgtgctgggt tttggcatag cctgtgtttg cagtgagagt gacttgctgt cagtcacggg
     1861 gcacagcctc ctgttcaccc actggcttcc cccttcccac tgaagtgtga gctctatgtt
     1921 tcagggcatc tattaaagag tgagccacag gcagcccctt gggagctctg gtttcatttg
     1981 ggggagaggg aggtgggggc ttagtttttg tgatctggct gactggaccc cgggaacagc
     2041 gctcctttga gaacgcccag acctaccaga tgaagcactt ggcatggctg caggccagca
     2101 ggggaggcct cagaggccca ggctgggcac gtgggtgccg agtctgcaaa ggacagcccc
     2161 atggctgtga gctgtcggct gcagggcgca gggcctgggg ggcaggcgtt tgggccacca
     2221 agctggacgt tagggtggcc acagagggcc aagcaggcag ggagcctgtg ccgccatcct
     2281 ggggggccac aggtgctctc tagaaatctg cagccatggc ggttttgggg tcaggggtct
     2341 aaaggcagct ctgagctgca gtgtagtttg tgatcaccca ggaggagggt gtagagcgga
     2401 tggacgggag gaagcggctg cagctggagg gaagagaggg gccgtccagc ctcacccgct
     2461 gcagtcagct ctcctatggg caaggaaaca gctaggtggc cctgacccct gcggtcctga
     2521 gagccggtgg ccttcagccc ctcttggaca ggtcatggtc tggaagcgcc cagcgcagga
     2581 tggattccag accagcatcc ctgcaacctt ttcagcccca ctggctgctg ctttaaactg
     2641 gcatggagac atttgatcag cttcccagcc agttttctct ccagctcagc caggagtggg
     2701 tgcctgtggc ctgtgtcacc aagagctggt gtctcctgca tgaggggcat gcacccctgg
     2761 aatgagggtc tgtgcctcga gctcaaagtg tcccggctct gtcatctgcc tgaagttgcc
     2821 tggcttttcc agtggggcct ccttgagtgc tgcagcatct gggagcatcc tagcctgtcc
     2881 cgtgggtgtg gctgggtgag ggctaggagg tgagccagga aggaagcact ccactccaga
     2941 gtgaggtgcc gcctgagcca tcctgtgccc cctcagtgtc ttcaggtctc tgcaggtgtc
     3001 aggatctcaa aatacttggt tcagatcaaa cacatttcgt gttcttctgt gtcctttttt
     3061 tttttttttt ttttttttga gatggagtct tgctctgttg gccaggctgg agctcagtgg
     3121 cacaatctct gctcactgca acctctgcct cccaggttcc agtgattttc ctgcctcagc
     3181 ctcctgggta gctgggacta caggcacatg ccccatgccc ggctaatttt tgtattttta
     3241 gtagagatgg ggttttacca tgttggccag gatggtctca attacaggca tgagccactg
     3301 tgcccagcct cttctgtgtc tttaaaaagg aaagtgtcag tcaacaaaag agcccctctg
     3361 tcatacaaac caaatcagtt ttcaccaaaa tgtccttagt cctctcagaa aggcctggcc
     3421 tttaggacct tggaagcagg ccctaaagcg catcggcagg gtcagagctt tgtgttcagg
     3481 aacactggga tctgctgaga gctggtgtag ccgctccaaa actgcacact gcttttcctg
     3541 tctgctttag tttgtgttgt aaaaacatag gaagttcgtg tgtgtgtgtg tgtgtgtgaa
     3601 ttcaaggcat aaggtgcagt tgtgttatgt gggtatagtg catggtggtg cagtctgggc
     3661 ttcttaccct cacacctcct gcctcccctc gttagagtcc cccacatccg ccttccactt
     3721 tctgagccca cattgcctgt tgggcagctc ctggttatga ggcaggacgt gtggtgtttg
     3781 actgtctgtt tctgagctgt tttacttaac acagtgtata tggtgcatct ttaggaaagc
     3841 atgcttgtat tttcctttca aagccagata tgggcgggtg tggtggctca tgcctgtgat
     3901 cccagcactt tgggaggccg aggcaggagg attgcttgag cccaggagtt tgagagcagc
     3961 ctgggcaaca tatcaagact ctgtctctta aaaaaaaaaa aattagccag gcatggtggt
     4021 ccacacctgt ggtcccacct attcgggagg ctgaagtgcg aggatcactt gagcccagga
     4081 ggttgaggct gcagtgagct gtgattgtgc cactgcactc cagcctgggc aacagagcaa
     4141 gaccctgtct taaaacaaag aaagaataaa aaagaagaga agttgcagtt ggtagtattt
     4201 ccagagcccg gcagacagtg tagaaactgt gagaaaccca cctccaaatc aggccttaga
     4261 tagaaaacat cctaagaagt atacgttcat tgtaaaatca ttatcatcat catcttctaa
     4321 tccccaagga gacaagctcc ctgttctgtg ttcttgtatg aggcggtcag tcaagcgcag
     4381 cccgagagga gaggcaggag cctgggagag cctctgctag acctttcctg gcacttcccc
     4441 aggaaaggca aggcagggca gatgtgcggc tggccagctg gagtgatttc catgggctcc
     4501 aagctgtagg agtggtccct ggctgccaga gtgctctggc cagatagagg tgggctctgg
     4561 actggtgagg ctgcattgcg gaggcgcgca cacccagctg ggcccttatc tgtaaggact
     4621 ggctggcctg ggaggggcag tctcccagcc agaaggtgga attttttttt ttttttgaga
     4681 taccaaaaca tcctaaggta caggaaagaa attgtcaaca cacacacaca gtaccataaa
     4741 caaggcccag cagaaaggca gacagaagca cacacaccaa tgttctgatc ttagagtaag
     4801 cagacacatc acatggaatg aggatgttta atatatttaa agaaaacaat ttatgcacag
     4861 gaagcaagag actataaaaa tgaccaagca gattttaaaa agagaacttg ttgaaataaa
     4921 aataaaatac tctaaaaaat attccatgat gtgtttcctg aagaatcggg aactgtttga
     4981 agaaatgacc caaagtgcag cacagaaaga cagagctgga aaatatgaga tgggatgaga
     5041 caagaagatg gagagtttag tatgacatcc gcaatttacg agtaattcca gaaggagggg
     5101 aaagagaata tggggtggag gcaagtattc agagaaatac ggctgagaac tttcccaaac
     5161 caacaggagc ccctgctcct taggttcagg aaccagcagc tcccaagcag catagttaaa
     5221 aagctctacc caaacagtca cgtcttggtg aaagtacaga atctcatgac aaagagatct
     5281 taaggcagaa aagaaagaga ttatctggaa agaagaaaat aagtagttga ttaaaagcaa
     5341 cagtggaagc cagaaagata gtaaataata tcttcaaagt cctgggagaa aatagtgata
     5401 gcctagaatt gtgtacccag aaaatccgtc tttcaagaat gaggggccag gcgcagtggc
     5461 tcacgtctgt attcccagca ctttgggagg ccaaggcagg tggatcacct gaggtcagga
     5521 gttcaagacc agcctgatca acatggtgaa accctgtctc tactaaaaat ataaaaatta
     5581 gctgggtgtg gtggcatatt cctgtaatcc cagctactcg ggaggctgag gcaggagaat
     5641 cacttgaacc caggaggcag aggttgcagt gagccaagat tgtgccattg cactccagcc
     5701 tgggcaatag aatgagactc tgtctcaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaggg
     5761 ctaaacgcat tttcatacca cgaaactgcc ctcactccgg agtttgctgc cagcagagcc
     5821 tagagcgtct aaggcatgtc tgggcttctg gcgagggatc ctgtggggaa gctggagatg
     5881 gaagaaggag tgaataggca ggaaggtgga tttgtgggca tgtctaaaac accacatcta
     5941 gaagcttcta cttagaatgc ctacttttgg agtttataaa aaagatgcca catgttgagt
     6001 tgggagagag gtgcccaaac ctacagtgtt ctaaagtccc cctactggtc ggaaggaggg
     6061 cacggacact gacttcagca tctgttacaa ggcttatcag agttttcaga taatctctaa
     6121 aagaatggaa atatatcttc caaactatag agggaagacc atggaattta aaagccagtc
     6181 cttcaaggag aaatgaacaa actcaaaatc gtagttggga gtgttttttt tttttttgag
     6241 acagagtctc actcttgccc aggctggagt ggagtggcat aaacacagct cactacagcc
     6301 tcaaccttct gggctcagat gattctcctg cctcagcctc ctgagtagca ggaactacag
     6361 gtgtgagtca tcacactcag ctaatttttt tttttttttt ttactttttg tagagatggg
     6421 gttctcactg cattgcccag gctggtcttg aatttctggg ctcaggtgat cttcccacct
     6481 cagcctcctg agtagctggg accacaggtg catgctaatt tttaaaattt tttgttttta
     6541 aatgctggtc ttcaactctt gtccccaagt agctgggatt ggggcatgag gcactgtgct
     6601 aacttcatgt tgaaattgaa ttgccattct aacagtgtta agagatgggg cctgtaagag
     6661 gtgattaggc tgtgagggct ccactctcca agggcggagt taatgttatg aaagggtaag
     6721 tccagcccgc ttgtgcctct cttgccctcc agtgttcaaa gcaccatctt ggaggtggaa
     6781 ttcccaagtc ggccagttcc tggatcttgg actgtccggt ctccagaacc gtgagccaat
     6841 aaacctctgt tccttgtaaa tgagtcagcc tcaggtattc tcttgtagca gcacaaatgg
     6901 aggcagacac ttccttcacc aatctcatgg gaggcccact cctgtgagct gcctctagtt
     6961 ccccaggcca cagactccat cagggctcac ctggagccgt ccctgtctca cctggaagct
     7021 ctcccactcc tctggccagc ctgggcatct ccacccaata tgagtgatgg cgactgattc
     7081 ccttacttta gcaagctggg aataaggaat gtttgcctgt tcttattcag gggtctctgc
     7141 atctccacac ttgcacaggt tgtgtgtaca tacattttcc tgtctttctg gtaagcccct
     7201 ggggtggagc tctgggccac agggcaggaa aactcagtgt tgctaggaac tgccagacat
     7261 tttcccggag tgagtgcagc atttacattc ccaccagaag tgtgccaggt tcttgtcata
     7321 ctgcgtcctc gccagcgctt ggtgctgtca gtcttcttag tttccattta gagattcagt
     7381 gtttaaattt tgaacgtctt gggtgaacac cttggtttga gaaaagtaag aaaaagggtc
     7441 tgtctggcta tgggccatgg agacccaggg cagtgctcca cgtgccaacc ctggggcagg
     7501 gagcccaagc taggactgtg actgtctggg ctgtgggcat ctgaccatct ccagtgtccc
     7561 cgtctttgat ttccctggcg atggtgctag cttagtttgt gcgcatcggc atttgtggga
     7621 gaaatgaagg attaatacat tttgttttat gtcagactct ctcagtgaga agttctcagg
     7681 gacaataata gcagggggtc ctgctgaaga aaaactggga gatggctgag ctctggtcca
     7741 tctttcattg attcattcat ttcttcattc attcaaccat gtattgaatg cttatagcca
     7801 atgaaaataa agtacacagt atatttta
//
