LOCUS       NM_002161               4483 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript
            variant 1, mRNA.
ACCESSION   NM_002161
VERSION     NM_002161.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Li,Y., Zhang,Y., Lu,X., Li,R., Peng,J. and Xu,Y.
  TITLE     Circular RNA IARS modulates the progression and ferroptosis of
            osteosarcoma via sponging miR-188-5p from RAB14
  JOURNAL   Neoplasma 71 (3), 279-288 (2024)
   PUBMED   38958715
  REMARK    GeneRIF: Circular RNA IARS modulates the progression and
            ferroptosis of osteosarcoma via sponging miR-188-5p from RAB14.
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 4483)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   4  (bases 1 to 4483)
  AUTHORS   Smigiel,R., Biela,M., Biernacka,A., Stembalska,A., Sasiadek,M.,
            Kosinska,J., Rydzanicz,M. and Ploski,R.
  TITLE     New evidence for association of recessive IARS gene mutations with
            hepatopathy, hypotonia, intellectual disability and growth
            retardation
  JOURNAL   Clin Genet 92 (6), 671-673 (2017)
   PUBMED   29052218
  REMARK    GeneRIF: Our report presents new evidence that recessive mutations
            inIARSgene are associated with characteristic clinical phenotype
            suchas infantile hepatopathy, growth retardation, facial
            dysmorphism,hypotonia, intellectual disability with no speech .
REFERENCE   5  (bases 1 to 4483)
  AUTHORS   Orenstein,N., Weiss,K., Oprescu,S.N., Shapira,R., Kidron,D.,
            Vanagaite-Basel,L., Antonellis,A. and Muenke,M.
  TITLE     Bi-allelic IARS mutations in a child with intra-uterine growth
            retardation, neonatal cholestasis, and mild developmental delay
  JOURNAL   Clin Genet 91 (6), 913-917 (2017)
   PUBMED   27891590
  REMARK    GeneRIF: bi-allelic mutations in cytosolic isoleucyl-tRNA
            synthetase (IARS) have been described in three individuals with
            growth delay, hepatic dysfunction, and neurodevelopmental
            disabilities. Here we report an additional subject with this
            condition identified by whole-exome sequencing. Our findings
            support the association between this disorder and neonatal
            cholestasis with distinct liver pathology
REFERENCE   6  (bases 1 to 4483)
  AUTHORS   Nichols,R.C., Raben,N., Boerkoel,C.F. and Plotz,P.H.
  TITLE     Human isoleucyl-tRNA synthetase: sequence of the cDNA, alternative
            mRNA splicing, and the characteristics of an unusually long
            C-terminal extension
  JOURNAL   Gene 155 (2), 299-304 (1995)
   PUBMED   7721108
REFERENCE   7  (bases 1 to 4483)
  AUTHORS   Lamour,V., Quevillon,S., Diriong,S., N'Guyen,V.C., Lipinski,M. and
            Mirande,M.
  TITLE     Evolution of the Glx-tRNA synthetase family: the glutaminyl enzyme
            as a case of horizontal gene transfer
  JOURNAL   Proc Natl Acad Sci U S A 91 (18), 8670-8674 (1994)
   PUBMED   8078941
REFERENCE   8  (bases 1 to 4483)
  AUTHORS   Shiba,K., Suzuki,N., Shigesada,K., Namba,Y., Schimmel,P. and
            Noda,T.
  TITLE     Human cytoplasmic isoleucyl-tRNA synthetase: selective divergence
            of the anticodon-binding domain and acquisition of a new structural
            unit
  JOURNAL   Proc Natl Acad Sci U S A 91 (16), 7435-7439 (1994)
   PUBMED   8052601
REFERENCE   9  (bases 1 to 4483)
  AUTHORS   Kaiser,E., Hu,B., Becher,S., Eberhard,D., Schray,B., Baack,M.,
            Hameister,H. and Knippers,R.
  TITLE     The human EPRS locus (formerly the QARS locus): a gene encoding a
            class I and a class II aminoacyl-tRNA synthetase
  JOURNAL   Genomics 19 (2), 280-290 (1994)
   PUBMED   8188258
REFERENCE   10 (bases 1 to 4483)
  AUTHORS   Norcum,M.T.
  TITLE     Structural analysis of the high molecular mass aminoacyl-tRNA
            synthetase complex. Effects of neutral salts and detergents
  JOURNAL   J Biol Chem 266 (23), 15398-15405 (1991)
   PUBMED   1651330
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL136097.10.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 11, 2019 this sequence version replaced NM_002161.5.
            
            Summary: Aminoacyl-tRNA synthetases catalyze the aminoacylation of
            tRNA by their cognate amino acid. Because of their central role in
            linking amino acids with nucleotide triplets contained in tRNAS,
            aminoacyl-tRNA synthetases are thought to be among the first
            proteins that appeared in evolution. Isoleucine-tRNA synthetase
            belongs to the class-I aminoacyl-tRNA synthetase family and has
            been identified as a target of autoantibodies in the autoimmune
            disease polymyositis/dermatomyositis. Alternatively spliced
            transcript variants have been found. [provided by RefSeq, Nov
            2012].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK293014.1, BX647236.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000443024.7/ ENSP00000406448.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                AL136097.10        128440-128526       c
            88-213              AL136097.10        124130-124255       c
            214-370             AL136097.10        122955-123111       c
            371-490             AL136097.10        122620-122739       c
            491-573             AL136097.10        121365-121447       c
            574-691             AL136097.10        120551-120668       c
            692-839             AL136097.10        115575-115722       c
            840-927             AL136097.10        113028-113115       c
            928-988             AL136097.10        112692-112752       c
            989-1084            AL136097.10        109255-109350       c
            1085-1207           AL136097.10        106362-106484       c
            1208-1299           AL136097.10        105814-105905       c
            1300-1398           AL136097.10        104714-104812       c
            1399-1525           AL136097.10        103003-103129       c
            1526-1599           AL136097.10        100309-100382       c
            1600-1794           AL136097.10        99758-99952         c
            1795-1881           AL136097.10        97798-97884         c
            1882-1965           AL136097.10        94980-95063         c
            1966-2110           AL136097.10        93683-93827         c
            2111-2231           AL136097.10        91509-91629         c
            2232-2323           AL136097.10        88191-88282         c
            2324-2401           AL136097.10        86637-86714         c
            2402-2523           AL136097.10        85542-85663         c
            2524-2626           AL136097.10        85016-85118         c
            2627-2710           AL136097.10        84687-84770         c
            2711-2885           AL136097.10        82206-82380         c
            2886-2998           AL136097.10        79788-79900         c
            2999-3094           AL136097.10        78045-78140         c
            3095-3271           AL136097.10        76983-77159         c
            3272-3377           AL136097.10        75685-75790         c
            3378-3503           AL136097.10        63830-63955         c
            3504-3647           AL136097.10        58175-58318         c
            3648-3800           AL136097.10        57349-57501         c
            3801-4483           AL136097.10        45036-45718         c
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q22.31"
     gene            1..4483
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /note="isoleucyl-tRNA synthetase 1"
                     /db_xref="GeneID:3376"
                     /db_xref="HGNC:HGNC:5330"
                     /db_xref="MIM:600709"
     exon            1..87
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            88..213
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..3883
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /EC_number="6.1.1.5"
                     /note="isoform 1 is encoded by transcript variant 1;
                     isoleucine tRNA ligase 1, cytoplasmic; isoleucine--tRNA
                     ligase, cytoplasmic"
                     /codon_start=1
                     /product="isoleucine--tRNA ligase, cytoplasmic isoform 1"
                     /protein_id="NP_002152.2"
                     /db_xref="CCDS:CCDS6694.1"
                     /db_xref="GeneID:3376"
                     /db_xref="HGNC:HGNC:5330"
                     /db_xref="MIM:600709"
                     /translation="MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFY
                     DGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGI
                     RGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVW
                     WVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETV
                     SLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEIL
                     ERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYR
                     VCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATT
                     FTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLK
                     DARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTI
                     PSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRG
                     WFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALR
                     LYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNE
                     NTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRR
                     RLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDT
                     LSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQD
                     PEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMT
                     SIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVL
                     LDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHT
                     EFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNIC
                     ANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNQTDLLS
                     LSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNG
                     LTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF
                     "
     exon            214..370
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            371..490
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            491..573
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            574..691
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            692..839
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            840..927
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            928..988
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1084
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1207
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1299
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1398
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1525
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1599
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1794
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            1795..1881
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..1965
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2110
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2231
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2323
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            2324..2401
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            2402..2523
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            2524..2626
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            2627..2710
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            2711..2885
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            2886..2998
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            2999..3094
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            3095..3271
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            3272..3377
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            3378..3503
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            3504..3647
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            3648..3800
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     exon            3801..4483
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4333..4338
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /note="hexamer: AACAAA"
     polyA_site      4350
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /note="major polyA site"
     regulatory      4464..4469
                     /regulatory_class="polyA_signal_sequence"
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
                     /note="hexamer: AATAAA"
     polyA_site      4483
                     /gene="IARS1"
                     /gene_synonym="GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785"
ORIGIN      
        1 gtgcgcccgg ttgcagcgtg gacgccggat gagttgcttt taggcttgct ggcccgcggg
       61 gctgtccagg cacgcgaggc ccctcagcaa caaaatgctt caacaagttc cagaaaacat
      121 aaattttcct gctgaagaag agaaaatctt ggagttttgg actgaattta attgttttca
      181 ggaatgctta aagcaatcaa aacataaacc aaaatttacc ttctatgatg gtcctccttt
      241 tgcaactgga ctgcctcact atggacatat acttgcgggt acaattaaag atatagttac
      301 aagatatgct caccagagtg ggtttcatgt tgacagaaga tttggatggg attgccatgg
      361 cttacctgtg gaatatgaaa ttgataagac actgggaatc agaggaccag aggatgtggc
      421 caaaatgggg attacagagt ataacaatca gtgccgagca attgtgatga gatattctgc
      481 tgagtggaag tctactgtta gcagacttgg ccgatggatt gactttgaca atgactataa
      541 aactctgtat ccacaattca tggaatcagt ctggtgggtc ttcaaacaac tctatgataa
      601 aggccttgtt tatagaggtg tgaaagtcat gcccttctct acggcatgta acactccact
      661 ttccaacttc gagtcacacc agaattataa ggatgttcaa gatccttcag tatttgtaac
      721 tttccctttg gaagaagatg aaactgtatc tttagttgct tggacaacca ctccctggac
      781 tctacctagt aaccttgctg tgtgtgttaa tccagaaatg caatatgtga aaattaaaga
      841 tgttgccaga ggacgattac tcattttaat ggaagccaga ttgtcagccc tctataaatt
      901 ggagagtgac tatgagatcc ttgaaagatt tcctggtgcc tatcttaaag gcaagaagta
      961 caggcccctg tttgactatt tcctgaagtg taaagagaat ggcgctttca ctgtgcttgt
     1021 tgacaactat gtgaaggaag aagaaggcac aggggttgtc caccaagctc cttacttcgg
     1081 tgctgaggac tatcgggtct gtatggactt taacattatt cggaaagact cactccctgt
     1141 ttgccctgtg gatgcttcag gctgcttcac aacggaggtg acagatttcg caggacagta
     1201 tgtgaaggat gctgacaaaa gtatcatcag gactttgaag gaacaaggcc gacttctggt
     1261 tgccaccacc ttcactcaca gctacccttt ttgctggaga tcagacactc ctctaattta
     1321 caaagcagtg cccagctggt ttgtgcgagt ggagaacatg gtggaccagc tcctaaggaa
     1381 caatgacctg tgctactggg tcccagagtt ggtacgagaa aaacgatttg gaaattggct
     1441 gaaagatgca cgtgactgga caatttccag aaacagatac tggggcaccc ccatcccact
     1501 gtgggtcagc gatgactttg aggaggtggt atgcattggg tcagtggcgg aacttgaaga
     1561 actgtcagga gcaaagatct cagatctcca cagagagagt gttgaccacc tgaccattcc
     1621 ttcacgctgt gggaagggat ccttgcaccg catctctgaa gtgtttgact gttggtttga
     1681 gagtggcagc atgccctatg ctcaggttca ttacccgttt gaaaacaaga gggagtttga
     1741 ggatgctttt cctgcagatt tcattgccga gggcatcgac caaaccagag gatggtttta
     1801 taccctgctg gtgctggcca cggccctctt tggacaaccg cctttcaaga acgtaattgt
     1861 gaatgggctt gtcctggcaa gtgatggcca aaaaatgagc aaacggaaaa agaattatcc
     1921 agatccagtt tccatcatcc agaagtatgg tgctgatgcc ctcagattat atctgattaa
     1981 ctcccctgtg gtgagagcag aaaacctccg ctttaaagaa gagggtgtgc gggacgtcct
     2041 taaggatgta ctgctcccat ggtacaatgc ctatcgcttc ttaatccaga acgttctgag
     2101 gctccagaag gaggaagaaa tagaatttct ctacaatgag aacacggtta gagaaagccc
     2161 caacattaca gaccggtgga tcctgtcctt catgcagtct ctcattggct tctttgagac
     2221 tgaaatggca gcttataggc tttatactgt ggtgcctcgc ctggtcaagt ttgtagatat
     2281 tctgaccaat tggtatgtta gaatgaaccg cagaagatta aagggtgaaa atgggatgga
     2341 ggattgtgtc atggccctag aaaccttgtt tagtgttctg ctttctcttt gcagacttat
     2401 ggctccctac acaccttttc tcactgaatt gatgtaccag aatctaaagg tgctgattga
     2461 ccctgtttct gttcaggaca aggacacact cagcattcac tacctcatgc tgccccgtgt
     2521 tcgagaagaa ttgattgaca agaaaacaga gagtgcagta tctcagatgc agtctgtgat
     2581 tgaacttgga agagtgatca gagaccgaaa aactattccc ataaagtatc ctttgaaaga
     2641 aattgtggtt atccatcaag atccagaagc tcttaaagat atcaagtctt tggagaagta
     2701 tatcattgag gaactcaatg ttcgaaaagt tacactgtct acagataaaa acaagtatgg
     2761 cattcggcta agggcagaac cagatcacat ggtcctgggg aagcgtctga agggagcctt
     2821 taaggcagtg atgacgtcca tcaagcagtt gagcagtgag gagctggagc agttccagaa
     2881 gactgggacc attgttgtgg aaggccatga attgcacgat gaagacatcc gcctcatgta
     2941 cacctttgat caggccacag gtgggactgc gcaatttgaa gcacactcag atgctcaggc
     3001 tttggtcctc ttagatgtca ctcctgacca gtcaatggta gatgaaggaa tggctcggga
     3061 agtcatcaat cgcatacaga aacttcgcaa aaagtgcaat ctggttccaa ctgatgaaat
     3121 cacagtgtac tataaagcaa agtctgaagg aacatatctg aatagtgtta ttgaaagcca
     3181 cacagagttc atatttacca ccataaaggc tcccttgaaa ccatatccag tttctccatc
     3241 ggataaagtc cttattcaag aaaaaacaca gttgaaggga tctgaactgg aaattacact
     3301 caccagagga tcttcccttc ctggtcctgc ttgtgcatat gtcaatctta acatttgtgc
     3361 aaatggcagt gaacaaggtg gagtattgct cctggaaaat ccaaaaggtg acaataggtt
     3421 ggacctttta aagctgaaga gtgttgtcac tagcattttt ggtgtgaaaa atacagagct
     3481 ggctgtcttc catgatgaaa cagaaataca aaaccaaact gacttactga gtcttagtgg
     3541 aaaaacactt tgtgtgactg caggatcggc tccctctctg atcaacagtt ctagtactct
     3601 tctttgtcag tatatcaacc tacagctcct gaatgcaaag ccacaagagt gtttaatggg
     3661 gacagtgggc actctcctgc ttgaaaaccc acttgggcag aatggactca cccaccaagg
     3721 tcttctgtat gaagcagcca aggtgtttgg ccttcggagc aggaagctaa agctgtttct
     3781 gaatgagacc caaacgcagg aaattacaga agacatcccc gtgaagactt tgaatatgaa
     3841 gactgtgtat gtttctgtgt taccaacaac agcagacttc tagcatgtac ttatcaatgt
     3901 tgttcggtca gcccttccct aattacacct atcccctaca catacatgca catagacaca
     3961 cacatgaaca cactgaagat atttccttca ggtgtgtgta aaatatgctg cttggattga
     4021 aattcaaatg ggattgatta gtcaagtaac ttgagacctc acagtaatct tcacacttaa
     4081 ccttagacac ctatgcagtc atgttgggag caggttacaa tgttacttca gcccacagtt
     4141 tatttctata cttgagttct taagtacaga agatagaagt gatttaaatg gcatagtata
     4201 tatatcattt tctggccttt taaaatttat ttgagacctc ttgatgaaat ggacatatta
     4261 tatatttctg ccacctggat tttcctggat aatttgatgg aatattttaa gtttcagtaa
     4321 atcagaacaa taaacaaact cagatataaa tgtgttgaaa gtaattcatt ctcccataga
     4381 taaatgaaac ttgaaatgtg taaactttga agaacataaa aacttcatgg tggtttctta
     4441 ttaagacagt ggactagaca tctaataaaa cttctttcca aaa
//
