LOCUS       NM_006389               4598 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens hypoxia up-regulated 1 (HYOU1), transcript variant 1,
            mRNA.
ACCESSION   NM_006389
VERSION     NM_006389.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Wang,W., Jiang,X., Xia,F., Chen,X., Li,G., Liu,L., Xu,Q., Zhu,M.
            and Chen,C.
  TITLE     HYOU1 promotes cell proliferation, migration, and invasion via the
            PI3K/AKT/FOXO1 feedback loop in bladder cancer
  JOURNAL   Mol Biol Rep 50 (1), 453-464 (2023)
   PUBMED   36348197
  REMARK    GeneRIF: HYOU1 promotes cell proliferation, migration, and invasion
            via the PI3K/AKT/FOXO1 feedback loop in bladder cancer.
            Erratum:[Mol Biol Rep. 2023 Jun;50(6):5527. doi:
            10.1007/s11033-023-08325-4.. PMID: 37072656]
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Arab,F., Rezaei,N., Taheri,F., Kouhpeikar,H., Rayzan,E.,
            Mirbeyk,M., Zare-Abdollahi,D. and Ghadami,M.
  TITLE     The Clinical and Molecular Assessment of Iranian Families with
            Severe Congenital Neutropenia, Identification of HYOU1 and SHOC2 as
            Potential Novel Gene Defects
  JOURNAL   Iran J Allergy Asthma Immunol 21 (3), 344-354 (2022)
   PUBMED   35822684
  REMARK    GeneRIF: The Clinical and Molecular Assessment of Iranian Families
            with Severe Congenital Neutropenia, Identification of HYOU1 and
            SHOC2 as Potential Novel Gene Defects.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4598)
  AUTHORS   Liu,J. and Wang,Y.
  TITLE     Long non-coding RNA KCNQ1OT1 facilitates the progression of
            cervical cancer and tumor growth through modulating
            miR-296-5p/HYOU1 axis
  JOURNAL   Bioengineered 12 (1), 8753-8767 (2021)
   PUBMED   34704918
  REMARK    GeneRIF: Long non-coding RNA KCNQ1OT1 facilitates the progression
            of cervical cancer and tumor growth through modulating
            miR-296-5p/HYOU1 axis.
REFERENCE   4  (bases 1 to 4598)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4598)
  AUTHORS   Wang,J.M., Jiang,J.Y., Zhang,D.L., Du,X., Wu,T. and Du,Z.X.
  TITLE     HYOU1 facilitates proliferation, invasion and glycolysis of
            papillary thyroid cancer via stabilizing LDHB mRNA
  JOURNAL   J Cell Mol Med 25 (10), 4814-4825 (2021)
   PUBMED   33792181
  REMARK    GeneRIF: HYOU1 facilitates proliferation, invasion and glycolysis
            of papillary thyroid cancer via stabilizing LDHB mRNA.
REFERENCE   6  (bases 1 to 4598)
  AUTHORS   Bando,Y., Ogawa,S., Yamauchi,A., Kuwabara,K., Ozawa,K., Hori,O.,
            Yanagi,H., Tamatani,M. and Tohyama,M.
  TITLE     150-kDa oxygen-regulated protein (ORP150) functions as a novel
            molecular chaperone in MDCK cells
  JOURNAL   Am J Physiol Cell Physiol 278 (6), C1172-C1182 (2000)
   PUBMED   10837345
REFERENCE   7  (bases 1 to 4598)
  AUTHORS   Ozawa,K., Kuwabara,K., Tamatani,M., Takatsuji,K., Tsukamoto,Y.,
            Kaneda,S., Yanagi,H., Stern,D.M., Eguchi,Y., Tsujimoto,Y., Ogawa,S.
            and Tohyama,M.
  TITLE     150-kDa oxygen-regulated protein (ORP150) suppresses
            hypoxia-induced apoptotic cell death
  JOURNAL   J Biol Chem 274 (10), 6397-6404 (1999)
   PUBMED   10037731
REFERENCE   8  (bases 1 to 4598)
  AUTHORS   Tsukamoto,Y., Kuwabara,K., Hirota,S., Kawano,K., Yoshikawa,K.,
            Ozawa,K., Kobayashi,T., Yanagi,H., Stern,D.M., Tohyama,M.,
            Kitamura,Y. and Ogawa,S.
  TITLE     Expression of the 150-kd oxygen-regulated protein in human breast
            cancer
  JOURNAL   Lab Invest 78 (6), 699-706 (1998)
   PUBMED   9645760
REFERENCE   9  (bases 1 to 4598)
  AUTHORS   Ikeda,J., Kaneda,S., Kuwabara,K., Ogawa,S., Kobayashi,T.,
            Matsumoto,M., Yura,T. and Yanagi,H.
  TITLE     Cloning and expression of cDNA encoding the human 150 kDa
            oxygen-regulated protein, ORP150
  JOURNAL   Biochem Biophys Res Commun 230 (1), 94-99 (1997)
   PUBMED   9020069
REFERENCE   10 (bases 1 to 4598)
  AUTHORS   Tsukamoto,Y., Kuwabara,K., Hirota,S., Ikeda,J., Stern,D.,
            Yanagi,H., Matsumoto,M., Ogawa,S. and Kitamura,Y.
  TITLE     150-kD oxygen-regulated protein is expressed in human
            atherosclerotic plaques and allows mononuclear phagocytes to
            withstand cellular stress on exposure to hypoxia and modified low
            density lipoprotein
  JOURNAL   J Clin Invest 98 (8), 1930-1941 (1996)
   PUBMED   8878445
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY089857.1, DC364982.1,
            U65785.1, AK301514.1, AK127324.1, BC072436.1 and AA580314.1.
            
            On Nov 23, 2018 this sequence version replaced NM_006389.4.
            
            Summary: The protein encoded by this gene belongs to the heat shock
            protein 70 family. This gene uses alternative transcription start
            sites. A cis-acting segment found in the 5' UTR is involved in
            stress-dependent induction, resulting in the accumulation of this
            protein in the endoplasmic reticulum (ER) under hypoxic conditions.
            The protein encoded by this gene is thought to play an important
            role in protein folding and secretion in the ER. Since suppression
            of the protein is associated with accelerated apoptosis, it is also
            suggested to have an important cytoprotective role in
            hypoxia-induced cellular perturbation. This protein has been shown
            to be up-regulated in tumors, especially in breast tumors, and thus
            it is associated with tumor invasiveness. This gene also has an
            alternative translation initiation site, resulting in a protein
            that lacks the N-terminal signal peptide. This signal
            peptide-lacking protein, which is only 3 amino acids shorter than
            the mature protein in the ER, is thought to have a housekeeping
            function in the cytosol. In rat, this protein localizes to both the
            ER by a carboxy-terminal peptide sequence and to mitochondria by an
            amino-terminal targeting signal. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Mar 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U65785.1, SRR1660809.146826.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000617285.5/ ENSP00000480150.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                HY089857.1         43-52
            11-91               DC364982.1         11-91
            92-2427             U65785.1           1-2336
            2428-2446           AK301514.1         2028-2046
            2447-3741           U65785.1           2356-3650
            3742-4114           AK127324.1         3655-4027
            4115-4421           U65785.1           4024-4330
            4422-4425           BC072436.1         2887-2890
            4426-4597           U65785.1           4332-4503
            4598-4598           AA580314.1         24-24               c
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.3"
     gene            1..4598
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /note="hypoxia up-regulated 1"
                     /db_xref="GeneID:10525"
                     /db_xref="HGNC:HGNC:16931"
                     /db_xref="MIM:601746"
     exon            1..186
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            187..284
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     CDS             194..3193
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; oxygen regulated protein (150kD); hypoxia
                     up-regulated protein 1; 150 kDa oxygen-regulated protein;
                     170 kDa glucose-regulated protein; epididymis secretory
                     sperm binding protein; heat shock protein family H member
                     4"
                     /codon_start=1
                     /product="hypoxia up-regulated protein 1 isoform 1
                     precursor"
                     /protein_id="NP_006380.1"
                     /db_xref="CCDS:CCDS8408.1"
                     /db_xref="GeneID:10525"
                     /db_xref="HGNC:HGNC:16931"
                     /db_xref="MIM:601746"
                     /translation="MADKVRRQRPRRRVCWALVAVLLADLLALSDTLAVMSVDLGSES
                     MKVAIVKPGVPMEIVLNKESRRKTPVIVTLKENERFFGDSAASMAIKNPKATLRYFQH
                     LLGKQADNPHVALYQARFPEHELTFDPQRQTVHFQISSQLQFSPEEVLGMVLNYSRSL
                     AEDFAEQPIKDAVITVPVFFNQAERRAVLQAARMAGLKVLQLINDNTATALSYGVFRR
                     KDINTTAQNIMFYDMGSGSTVCTIVTYQMVKTKEAGMQPQLQIRGVGFDRTLGGLEME
                     LRLRERLAGLFNEQRKGQRAKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLM
                     DDVDFKAKVTRVEFEELCADLFERVPGPVQQALQSAEMSLDEIEQVILVGGATRVPRV
                     QEVLLKAVGKEELGKNINADEAAAMGAVYQAAALSKAFKVKPFVVRDAVVYPILVEFT
                     REVEEEPGIHSLKHNKRVLFSRMGPYPQRKVITFNRYSHDFNFHINYGDLGFLGPEDL
                     RVFGSQNLTTVKLKGVGDSFKKYPDYESKGIKAHFNLDESGVLSLDRVESVFETLVED
                     SAEEESTLTKLGNTISSLFGGGTTPDAKENGTDTVQEEEESPAEGSKDEPGEQVELKE
                     EAEAPVEDGSQPPPPEPKGDATPEGEKATEKENGDKSEAQKPSEKAEAGPEGVAPAPE
                     GEKKQKPARKRRMVEEIGVELVVLDLPDLPEDKLAQSVQKLQDLTLRDLEKQEREKAA
                     NSLEAFIFETQDKLYQPEYQEVSTEEQREEISGKLSAASTWLEDEGVGATTVMLKEKL
                     AELRKLCQGLFFRVEERKKWPERLSALDNLLNHSSMFLKGARLIPEMDQIFTEVEMTT
                     LEKVINETWAWKNATLAEQAKLPATEKPVLLSKDIEAKMMALDREVQYLLNKAKFTKP
                     RPRPKDKNGTRAEPPLNASASDQGEKVIPPAGQTEDAEPISEPEKVETGSEPGDTEPL
                     ELGGPGAEPEQKEQSTGQKRPLKNDEL"
     exon            285..378
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            379..457
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            458..612
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            613..689
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            690..871
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            872..987
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1180
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1315
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1398
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1531
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1719
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            1720..1858
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..1919
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..1999
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            2000..2185
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            2186..2358
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2446
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2569
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            2570..2703
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            2704..2788
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            2789..3029
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            3030..3080
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            3081..3131
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     exon            3132..4598
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4578..4583
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /note="hexamer: AATAAA"
     polyA_site      4598
                     /gene="HYOU1"
                     /gene_synonym="GRP-170; Grp170; HSP12A; IMD59; ORP-150;
                     ORP150"
                     /note="major polyA site"
ORIGIN      
        1 aatgagcgcg cgccgcgtcc ggggccggct ggtgcgcgag acgccgccga gaggttggtg
       61 gctaatgtaa cagtttgcaa accgagagga gttgtgaagg gcgcgggtgg ggggcgctgc
      121 cggcctcgtg ggtacgttcg tgccgcgtct gtcccagagc tggggccgca ggagcggagg
      181 caagaggggc actatggcag acaaagttag gaggcagagg ccgaggaggc gagtctgttg
      241 ggccttggtg gctgtgctct tggcagacct gttggcactg agtgatacac tggcagtgat
      301 gtctgtggac ctgggcagtg agtccatgaa ggtggccatt gtcaaacctg gagtgcccat
      361 ggaaattgtc ttgaataagg aatctcggag gaaaacaccg gtgatcgtga ccctgaaaga
      421 aaatgaaaga ttctttggag acagtgcagc aagcatggcg attaagaatc caaaggctac
      481 gctacgttac ttccagcacc tcctggggaa gcaggcagat aacccccatg tagctcttta
      541 ccaggcccgc ttcccggagc acgagctgac tttcgaccca cagaggcaga ctgtgcactt
      601 tcagatcagc tcgcagctgc agttctcacc tgaggaagtg ttgggcatgg ttctcaatta
      661 ttctcgttct ctagctgaag attttgcaga gcagcccatc aaggatgcag tgatcaccgt
      721 gccagtcttc ttcaaccagg ccgagcgccg agctgtgctg caggctgctc gtatggctgg
      781 cctcaaagtg ctgcagctca tcaatgacaa caccgccact gccctcagct atggtgtctt
      841 ccgccggaaa gatattaaca ccactgccca gaatatcatg ttctatgaca tgggctcagg
      901 cagcaccgta tgcaccattg tgacctacca gatggtgaag actaaggaag ctgggatgca
      961 gccacagctg cagatccggg gagtaggatt tgaccgtacc ctggggggcc tggagatgga
     1021 gctccggctt cgagaacgcc tggctgggct tttcaatgag cagcgcaagg gtcagagagc
     1081 aaaggatgtg cgggagaacc cgcgtgccat ggccaagctg ctgcgtgagg ctaatcggct
     1141 caaaaccgtc ctcagtgcca acgctgacca catggcacag attgaaggcc tgatggatga
     1201 tgtggacttc aaggcaaaag tgactcgtgt ggaatttgag gagttgtgtg cagacttgtt
     1261 tgagcgggtg cctgggcctg tacagcaggc cctccagagt gccgaaatga gtctggatga
     1321 gattgagcag gtgatcctgg tgggtggggc cactcgggtc cccagagttc aggaggtgct
     1381 gctgaaggcc gtgggcaagg aggagctggg gaagaacatc aatgcagatg aagcagccgc
     1441 catgggggca gtgtaccagg cagctgcgct cagcaaagcc tttaaagtga agccatttgt
     1501 cgtccgagat gcagtggtct accccatcct ggtggagttc acgagggagg tggaggagga
     1561 gcctgggatt cacagcctga agcacaataa acgggtactc ttctctcgga tggggcccta
     1621 ccctcaacgc aaagtcatca cctttaaccg ctacagccat gatttcaact tccacatcaa
     1681 ctacggcgac ctgggcttcc tggggcctga agatcttcgg gtatttggct cccagaatct
     1741 gaccacagtg aagctaaaag gggtgggtga cagcttcaag aagtatcctg actacgagtc
     1801 caagggcatc aaggctcact tcaacctgga tgagagtggc gtgctcagtc tagacagggt
     1861 ggagtctgta tttgagacac tggtagagga cagcgcagaa gaggaatcta ctctcaccaa
     1921 acttggcaac accatttcca gcctgtttgg aggcggtacc acaccagatg ccaaggagaa
     1981 tggtactgat actgtccagg aggaagagga gagccctgca gaggggagca aggacgagcc
     2041 tggggagcag gtggagctca aggaggaagc tgaggcccca gtggaggatg gctctcagcc
     2101 cccaccccct gaacctaagg gagatgcaac ccctgaggga gaaaaggcca cagaaaaaga
     2161 aaatggggac aagtctgagg cccagaaacc aagtgagaag gcagaggcag ggcctgaggg
     2221 cgtcgctcca gccccagagg gagagaagaa gcagaagccc gccaggaagc ggcgaatggt
     2281 agaggagatc ggggtggagc tggttgttct ggacctgcct gacttgccag aggataagct
     2341 ggctcagtcg gtgcagaaac ttcaggactt gacactccga gacctggaga agcaggaacg
     2401 ggaaaaagct gccaacagct tggaagcatt catatttgag acccaggaca agctgtacca
     2461 gcccgagtac caggaagtgt ccacagagga gcagcgtgag gagatctctg ggaagctcag
     2521 cgccgcatcc acctggctgg aggatgaggg tgttggagcc accacagtga tgttgaagga
     2581 gaagctggct gagctgagga agctgtgcca agggctgttt tttcgggtag aggagcgcaa
     2641 gaagtggccc gaacggctgt ctgccctcga taatctcctc aaccattcca gcatgttcct
     2701 caagggggcc cggctcatcc cagagatgga ccagatcttc actgaggtgg agatgacaac
     2761 gttagagaaa gtcatcaatg agacctgggc ctggaagaat gcaactctgg ccgagcaggc
     2821 taagctgccc gccacagaga agcctgtgtt gctctcaaaa gacattgaag ctaagatgat
     2881 ggccctggac cgagaggtgc agtatctgct caataaggcc aagtttacca agccccggcc
     2941 ccggcctaag gacaagaatg ggacccgggc agagccaccc ctcaatgcca gtgccagtga
     3001 ccagggggag aaggtcatcc ctccagcagg ccagactgaa gatgcagagc ccatttcaga
     3061 acctgagaaa gtagagactg gatccgagcc aggagacact gagcctttgg agttaggagg
     3121 tcctggagca gaacctgaac agaaagaaca atcgacagga cagaagcggc ctttgaagaa
     3181 cgacgaacta taacccccac ctctgttttc cccattcatc tccaccccct tcccccacca
     3241 cttctattta tttaacatcg agggttgggg gaggggttgg tcctgccctc ggctggagtt
     3301 cctttctcac ccctgtgatt tggaggtgtg gagaagggga agggagggac agctcactgg
     3361 ttccttctgc agtacctctg tggttaaaaa tggaaactgt tctcctcccc agccccactc
     3421 cctgttccct acccatatag gccctaaatt tgggaaaaat cactattaat ttctgaatcc
     3481 tttgcctgtg ggtaggaaga gaatggctgc cagtggctga tgggtcccgg tgatgggaag
     3541 ggtatcaggt tgctggggag tttccactct tctctggtga ttgttccttc cctcccttcc
     3601 tctcccacca tgcgatgagc atcctttcag gccagtgtct gcagagcctc agttaccagg
     3661 tttggtttct gagtgcctat ctgtgctctt tcctccctct gcgggcttct cttgctctga
     3721 gcctcccttc cccattccca tccagctcct ttccccctgg gtttccttgg cttcctgcag
     3781 caaattgggc agttctctgc cccttgccta aaagcctgta cctctggatt ggcggaagta
     3841 aatctggaag gattctcact cgtatttccc acccctagtg gccagaggag ggaggggcac
     3901 agtgaagaag ggagcccacc acctctccga agaggaaagc cacgtagagt ggttggcatg
     3961 gggtgccagc atcgtgcaag ctctgtcata atctgcatct tcccagcagc ctggtacccc
     4021 aggttcctgt aactccctgc ctcctcctct cttctgctgt tctgctcctc ccagacagag
     4081 cctttccctc accccctgac cccctgggct gaccaaaatg tgctttctac tgtgagtccc
     4141 tatcccaaga tcctggggaa aggagagacc atggtgtgaa tgtagagatg ccacctccct
     4201 ctctctgagg caggcctgtg gatgaaggag gagggtcagg gctggccttc ctctgtgcat
     4261 cactctgcta ggttgggggc ccccgaccca ccatacctac gcctagggag cccgtcctcc
     4321 agtattccgt ctgtagcagg agctagggct gctgcctcag ctccaagaca agaatgaacc
     4381 tggctgtgtc agtcattttg tcttttcctt tttttttttt ttttgccaca ttggcagaga
     4441 tgggacctaa gggtcccacc cctcacccca cccccacctc ttctgtatgt ttgaattctt
     4501 tcagtagctg ttgatgctgg ttggacaggt ttgagtcaaa ttgtactttg ctccattgtt
     4561 aattgagaaa ctgtttcaat aaaatattct tttctaca
//
