LOCUS       NM_032581              13178 bp    mRNA    linear   PRI 10-OCT-2024
DEFINITION  Homo sapiens hyccin PI4KA lipid kinase complex subunit 1 (HYCC1),
            transcript variant 1, mRNA.
ACCESSION   NM_032581
VERSION     NM_032581.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Wang,Y., Ai,D. and Li,S.
  TITLE     CirRNA circFAM126A Exerts Oncogenic Functions in NSCLC to
            Upregulate IRS2
  JOURNAL   Biochem Genet 60 (6), 2364-2382 (2022)
   PUBMED   35397054
  REMARK    GeneRIF: CirRNA circFAM126A Exerts Oncogenic Functions in NSCLC to
            Upregulate IRS2.
REFERENCE   2  (bases 1 to 13178)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 13178)
  AUTHORS   Sun,M., Zhao,Y., Han,M., Zhang,B., Zhang,X., Zhang,Q., Lim,N.K.,
            Wang,W.A. and Huang,F.D.
  TITLE     TTC7 and Hyccin Regulate Neuronal Abeta42 Accumulation and its
            Associated Neural Deficits in Abeta42-Expressing Drosophila
  JOURNAL   J Alzheimers Dis 65 (3), 1001-1010 (2018)
   PUBMED   30103315
  REMARK    GeneRIF: Downregulation of Drosophila TTC7 and Hyccin also reduces
            neuronal Abeta accumulation and associated synaptic and motor
            defects as well as premature death in Abeta42-expressing flies,
            while overexpression of TTC7 and Hyccin produced the opposite
            effect.
REFERENCE   4  (bases 1 to 13178)
  AUTHORS   Lees,J.A., Zhang,Y., Oh,M.S., Schauder,C.M., Yu,X., Baskin,J.M.,
            Dobbs,K., Notarangelo,L.D., De Camilli,P., Walz,T. and
            Reinisch,K.M.
  TITLE     Architecture of the human PI4KIIIalpha lipid kinase complex
  JOURNAL   Proc Natl Acad Sci U S A 114 (52), 13720-13725 (2017)
   PUBMED   29229838
REFERENCE   5  (bases 1 to 13178)
  AUTHORS   Jiang,Y. and Zhang,H.
  TITLE     Propensity score-based nonparametric test revealing genetic
            variants underlying bipolar disorder
  JOURNAL   Genet Epidemiol 35 (2), 125-132 (2011)
   PUBMED   21254220
REFERENCE   6  (bases 1 to 13178)
  AUTHORS   Ugur,S.A. and Tolun,A.
  TITLE     A deletion in DRCTNNB1A associated with hypomyelination and
            juvenile onset cataract
  JOURNAL   Eur J Hum Genet 16 (2), 261-264 (2008)
   PUBMED   17928815
  REMARK    GeneRIF: A large intragenic deletion of DRCTNNB1A does not lead to
            congenital cataract in all of the patients in an afflicted family.
REFERENCE   7  (bases 1 to 13178)
  AUTHORS   Zara,F., Biancheri,R., Bruno,C., Bordo,L., Assereto,S.,
            Gazzerro,E., Sotgia,F., Wang,X.B., Gianotti,S., Stringara,S.,
            Pedemonte,M., Uziel,G., Rossi,A., Schenone,A., Tortori-Donati,P.,
            van der Knaap,M.S., Lisanti,M.P. and Minetti,C.
  TITLE     Deficiency of hyccin, a newly identified membrane protein, causes
            hypomyelination and congenital cataract
  JOURNAL   Nat Genet 38 (10), 1111-1113 (2006)
   PUBMED   16951682
  REMARK    GeneRIF: Mutation of this gene results in defective myelination of
            both the central and peripheral nervous system.
REFERENCE   8  (bases 1 to 13178)
  AUTHORS   Kawasoe,T., Furukawa,Y., Daigo,Y., Nishiwaki,T., Ishiguro,H.,
            Fujita,M., Satoh,S., Miwa,N., Nagasawa,Y., Miyoshi,Y., Ogawa,M. and
            Nakamura,Y.
  TITLE     Isolation and characterization of a novel human gene, DRCTNNB1A,
            the expression of which is down-regulated by beta-catenin
  JOURNAL   Cancer Res 60 (13), 3354-3358 (2000)
   PUBMED   10910037
REFERENCE   9  (bases 1 to 13178)
  AUTHORS   Vanderver,A., Tonduti,D., Schiffmann,R., Schmidt,J. and van der
            Knaap,M.S.
  TITLE     Leukodystrophy Overview - RETIRED CHAPTER, FOR HISTORICAL REFERENCE
            ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24501781
REFERENCE   10 (bases 1 to 13178)
  AUTHORS   Wolf,N.I., Biancheri,R., Zara,F., Bruno,C., Gazzerro,E., Rossi,A.,
            van der Knaap,M.S. and Minetti,C.
  TITLE     Hypomyelination and Congenital Cataract
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301737
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB099987.1, BC018710.1,
            AL833296.1, AA811006.1 and AC005682.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_032581.3.
            
            Summary: The protein encoded by this gene may play a part in the
            beta-catenin/Lef signaling pathway. Expression of this gene is
            down-regulated by beta-catenin. Defects in this gene are a cause of
            hypomyelination with congenital cataract (HCC). [provided by
            RefSeq, Oct 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.137060.1,
                                           SRR1803616.164150.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000432176.7/ ENSP00000403396.2
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 DB099987.1         66-66
            2-3750              BC018710.1         1-3749
            3751-6120           AL833296.1         3936-6305
            6121-6130           AA811006.1         15-24               c
            6131-13178          AC005682.2         26038-33085
FEATURES             Location/Qualifiers
     source          1..13178
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p15.3"
     gene            1..13178
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /note="hyccin PI4KA lipid kinase complex subunit 1"
                     /db_xref="GeneID:84668"
                     /db_xref="HGNC:HGNC:24587"
                     /db_xref="MIM:610531"
     exon            1..206
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            207..285
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /inference="alignment:Splign:2.1.0"
     CDS             235..1800
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /note="isoform 1 is encoded by transcript variant 1; down
                     regulated by Ctnnb1, a; hyccin; down-regulated by CTNNB1
                     protein A; family with sequence similarity 126 member A"
                     /codon_start=1
                     /product="hyccin isoform 1"
                     /protein_id="NP_115970.2"
                     /db_xref="CCDS:CCDS5377.1"
                     /db_xref="GeneID:84668"
                     /db_xref="HGNC:HGNC:24587"
                     /db_xref="MIM:610531"
                     /translation="MFTSEKGVVEEWLSEFKTLPETSLPNYATNLKDKSSLVSSLYKV
                     IQEPQSELLEPVCHQLFEFYRSGEEQLLQFTLQFLPELIWCYLAVSASRNVHSSGCIE
                     ALLLGVYNLEIVDKQGHTKVLSFTIPSLSKPSVYHEPSSIGSMALTESALSQHGLSKV
                     VYSGPHPQREMLTAQNRFEVLTFLLLCYNAALTYMPSVSLQSLCQICSRICVCGYPRQ
                     HVRKYKGISSRIPVSSGFMVQMLTGIYFAFYNGEWDLAQKALDDIIYRAQLELYPEPL
                     LVANAIKASLPHGPMKSNKEGTRCIQVEITPTSSRISRNAVTSMSIRGHRWKRHGNTE
                     LTGQEELMEISEVDEGFYSRAASSTSQSGLSNSSHNCSNKPSIGKNHRRSGGSKTGGK
                     EKETTGESCKDHFARKQTQRAQSENLELLSLKRLTLTTSQSLPKPSSHGLAKTAATVF
                     SKSFEQVSGVTVPHNPSSAVGCGAGTDANRFSACSLQEEKLIYVSERTELPMKHQSGQ
                     QRPPSISITLSTD"
     exon            286..387
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            388..567
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            568..648
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            649..764
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            765..860
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            861..977
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1065
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1225
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..13178
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5956..5961
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /note="hexamer: AATAAA"
     polyA_site      5976
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /note="major polyA site"
     regulatory      6012..6017
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /note="hexamer: AATAAA"
     polyA_site      6037
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /note="major polyA site"
     regulatory      6111..6116
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /note="hexamer: AATAAA"
     polyA_site      6130
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /note="major polyA site"
     regulatory      12758..12763
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
                     /note="hexamer: AATAAA"
     polyA_site      12786
                     /gene="HYCC1"
                     /gene_synonym="DRCTNNB1A; FAM126A; HCC; HLD5"
ORIGIN      
        1 agagaggctg gcggcagtgg tggctctccc ggctgggttg tcagtcagtg agaggagggg
       61 gaagatggct caccgggacc cgttctgcta ggaagaaggg gagcaggtgg tgctgctact
      121 cgggaggaag cagagcagcc tccgtcgctg tcgctgagag cctcacgagt cgaggtcctg
      181 caggtggagc tccggcagcc gccctggttg aaggtttaga acagatgtgg aaaaatgttt
      241 acttcagaga aaggggttgt ggaggaatgg ttgtcagagt ttaagacatt accagaaaca
      301 tctttaccaa attatgccac aaatttgaaa gacaagagtt ctttagtttc atctctctat
      361 aaagttatcc aggagccaca aagtgagttg ctagaacctg tctgtcacca gctctttgaa
      421 ttctatcgca gtggagagga gcagttgctt caatttacgc tgcaatttct cccagaacta
      481 atttggtgct accttgcagt ctcagccagc agaaatgtgc atagcagtgg atgcattgaa
      541 gctcttcttc taggggttta caatttggaa atagttgaca aacagggaca taccaaagta
      601 ttgagtttta cgattccatc tttatccaaa ccatctgtat accatgaacc ttccagcatt
      661 gggtccatgg ctctgactga gagtgcacta tcccagcatg gtttgtcaaa agtggtatac
      721 agtggacctc atcctcaaag ggagatgctg acagcacaga atcggtttga agtgttgaca
      781 ttccttttgt tgtgttacaa tgctgcctta acttacatgc ccagtgtttc tcttcagtca
      841 ctgtgtcaaa tttgttcaag aatctgtgtt tgtggatatc ctcgacaaca tgtaagaaaa
      901 tataaaggta taagtagcag gataccagtt tcttcaggat tcatggtgca aatgttaaca
      961 gggatttatt ttgcctttta taatggagaa tgggatctag ctcaaaaagc actggatgat
     1021 attatataca gagcccagct agaattatat ccagagccat tgctggttgc taatgcaata
     1081 aaggcttcat tgcctcatgg tcccatgaaa tctaataagg aaggtacaag gtgtattcaa
     1141 gttgaaatca caccaacttc ctctcgaata tcaagaaatg cagtaaccag catgtcaata
     1201 aggggtcata ggtggaaaag acatggaaat acagaattaa caggtcaaga agaactgatg
     1261 gagatatctg aagttgacga gggattttat tccagggctg cgtctagtac cagccagtcg
     1321 ggtttatcaa acagtagtca caattgtagt aacaagccaa gtataggaaa gaaccacaga
     1381 cggtcaggag gaagcaaaac tggaggaaaa gaaaaagaaa ctacagggga atcttgcaaa
     1441 gatcactttg ctcgaaaaca aactcagaga gcccaaagtg agaatcttga gcttctctct
     1501 ttgaagagac ttactttaac aaccagccaa tccctaccta agcctagtag ccatggtttg
     1561 gctaagaccg cagcgactgt atttagtaaa tcctttgaac aagtcagtgg tgtcacagtc
     1621 ccacataacc cgtcatctgc tgttggttgt ggggctggga cagatgccaa taggttttcc
     1681 gcttgtagtc tccaagaaga aaagcttatt tacgtttcag aaagaactga acttccaatg
     1741 aagcatcaat caggtcagca gagacctcct agtattagca ttactctgtc cacagattaa
     1801 ttagtaacat atttttctcc cataacctag tgaacctgga aatacaactt tgcttcttta
     1861 tgaaagtacc ctgggtcttt catccgtatt cctgacagga gccctgatgt cttaaattct
     1921 gaaggctccc ctgactttat gaagggaaga ttgtctaggt tgcagtaagt tgaaatatgg
     1981 ttttgttatt tcttgattaa gattttttcc ccttggtttg agtctttttt ttccctcctc
     2041 ttatttgtta ttgttgccct aagattgtca gtgacaataa attttgacag gttgtctaat
     2101 caagttggtt atgtctcatg tgaggttgta aggtggtttt tcttgttttg gtttggtttg
     2161 gttttttaat agctcctatt ttgatggctg tgtgtttggc cttcagagtc aaagggagtc
     2221 tgcatgtatg ggaactgttt tatagagaat ggggcagagg cacaactcca ggaattctct
     2281 agctcctggt caggaagcca atctgaaaac aggcatgtgg aaacacaaaa atgatgtgta
     2341 tgaaaagcac ctgatgaaaa tatttgtaag cagatcatct ttatgattag aacaatttgc
     2401 tcaaagccct ggaaaatgcc tggtaaccca gtgcctagaa tattatatga ttccagggga
     2461 tggctagagg catttctgtt gttatctctg aaacattttt atatcaaaaa tcagattgct
     2521 ggtaggtgtg ttgttttgaa gtgttttatc tggatttagc atgtgaagat ccctttcaat
     2581 tattaaggta taataggctg taaagctgat ttaccctact gctggacttt ggaaatccta
     2641 gttagtttgg actcctataa ttcagagcat acgtcattgg aagacttcct ggaaatcagt
     2701 gtaaatcttg caacatagct ctcaagatac cactgtataa tatgtaattg tttgtctgca
     2761 aatgcatttt tcatctataa aatgtcatga aagaaaataa aggtagtgaa atatggacaa
     2821 aggacattta tatattaact aaagttgttt atgtttgtgt tcttaaattt aaaacacgaa
     2881 gaaaaagaaa tgtctttctt gttagtgaac aggcctgttt taaatatctt agtatgatat
     2941 caaaagaata atcaatttag ttacctccca actaaaaagt aaaaatgaaa aagacgacat
     3001 ggcagaagtt gaaacaaact tgtatgtatc tgtatgtgac ctctagatgg aaataaaagt
     3061 ttgatgtttg ctagccgtgg tccttttggc tatggttgcg gttcccctgt aaagcaaatg
     3121 tacttaagtc atcattcaga gtgagagaga gaaagcacgg ctgaggctga gtcagttgac
     3181 cgttgaggaa gacctaagga gctgtccagt tcttggctct ttttcactgt gactgcacag
     3241 cggtttcctt cttcatagtc ccttgctgta gtcaccaaga gagcatgcta gtgttttcct
     3301 cttgttcctg cttatgcaga gctcagatat atgaaaaatt tgtatgcttt gaaggctgcc
     3361 aaagcattta tactctggac atagttttaa ctgcaactgc tttggctacc agtttaaacc
     3421 gagttcttca aaaagcatga actcgttttc attcattagc cctgtttaca atgtggattt
     3481 tgacataaat gaatttgatt ttcttttagc tccagtaaca gaatggaata agatctattg
     3541 ccataaattg tagttaagaa ctgtaaggca atcgggcagt gaaacttgat attgtattaa
     3601 cttaatagga accctgtgta caatttaaaa tatttgtaaa atatcactgt gaatttacag
     3661 atgtcttttt gtcattcttt aacaatgaat ttcccctgtg catagttatt aatagtattg
     3721 gtattattaa aaactaattt ggcattttaa gattggcatt ttaagcaagt tgaggtgctt
     3781 aggaaacatt gtagaataag tttggggaaa tgttagctga cagagctgag agatcagatt
     3841 ttcatgttct atcactgtaa tggaacctca tttagacctc tctagacctt tgctagctgt
     3901 gtggtctgag gaccagtaag taggattggc attatctggt atcttgttaa aaatgcagaa
     3961 tttcatgcca ctctaggtct actaaatcag aatttgcatt cttaacaagg ttcccaagtg
     4021 atttttatga gaagaaagta tcagagtgca aacaaattac tcgtctaata tgtagaattc
     4081 ccagtggtct cacagcccta gatcaaatgt gtcagacact agatcattat aatcttcatt
     4141 ggttttgaca gctaattaat tagtttaata atattgacat atggcttctt aagtatttga
     4201 attctagatt gctaatgatc cttatttttg cagtaaaaaa gtaacacttt atatttttct
     4261 tgttaactat aaatggaatc agagctgtaa atgcctgaaa ttttaagtgt gaaaaggggt
     4321 gccacccttt tatatactgc acaacaaatt tgcatttgta tattcttttt aaaaaatctc
     4381 tgaagacttg ctcagtgaaa tggttctgat tttttgcttc aaatgatttg cactaattga
     4441 ttttctggag agcggagtta cgttaacaac aaaattcttt tatagttcct ttgatactat
     4501 ttcaaataag gattacacat tgaaagtttt tatagaaatt atgaaaattg gataatctta
     4561 actatggtaa ccattactat tatccatact actttctccc cactgaactt tcaaatttga
     4621 aaattggggt agcttttgta ggtcactttc ttataaagat gctgatggtc acagatctcc
     4681 taagtatcca gaataatatt tttatcatga gaaggattcc acacaaaatt ggggtcttgc
     4741 accccatatt tcagaggatc tgaacagaaa ttgtggcttc attgcacatt ttaaatctct
     4801 atagattctt gaaatcaccc aacctctcac atttgaaaac ccaacggcct ggagtattct
     4861 ggtggcactg gagccagcac tagctttttg gaatccaagt ggggagagaa acaaaaatag
     4921 acttttcaag ggactgtccc agactaccca gggcaaatgt cttcccttcc cttcctgaag
     4981 ccacaggaaa gcaccctgga gtcctttgta taccactatg gaaattttta aggcaagctc
     5041 tcaagacttg aagagttgga attctcggca tttaaatgat gcctgaagtt tgttttgaaa
     5101 aagcaatatt tcattgctct tttcaattgt gaagcaactg ctcagcaaat attactcatt
     5161 tttttaaaaa gttgaattaa agggaaatat atttttaaaa ccaaaataga tcaaaatgat
     5221 cccagtttta agggattttg cctattttgg aagaataaaa ctaaaatgtt aaaactgttg
     5281 aattacagag aacagatgta ctctgagaca taattttaaa caaatattta aaataaggca
     5341 ggttaacatt tgcgtttagg cacaataaat ctgtattaaa gggaagcaca tcaaggaaat
     5401 atatacatgt tgaataatgt aactgaaaaa tattttttaa aaaccactta aaaagaaaaa
     5461 taatttgatg ggtactttaa gcattgtaga tagaaattaa tgtataatag tgtcctccca
     5521 gtctttgtat gaaaaattaa aactctctag tcctttaatg agcatgaatt tatacttcta
     5581 catttgttgc ttagtaaaat tatactcagt actttgagtt attcagattt atgtttaata
     5641 ttgttaaatt cttcatcaga gagcataaat atattgtctt ctatactttg taacttgtgc
     5701 atatctaaag aaactaacaa cgaacatttt gggagatgtt tttgtgtttt tttatagtag
     5761 agttttagag tacatttgtc ttaataggaa gtttttacca taaacccaag attttatttc
     5821 acgtattgtt gctttctctt atatggaact tattgtgtac ctcttacctt tatttctaat
     5881 catttagtga tactaatctt ttcatagtaa atatatttgt ttctaattgt attcctagag
     5941 tttacattcc taaagaataa acgacgactt tggctatttt tgtgtgttcc ctaccttctt
     6001 aaggctatgg aaataaactg gagtttagtt ttttgaattt ttgtatattt gtgtaaatgt
     6061 tctccatatg tagtacttta tacaacaagt gttgcctctt gttttattga aataaaaact
     6121 gaaaaaaata ttttttccag atcagtttac tcatttaaaa aattattata aaaatttgca
     6181 gaagttttga tatgaaggaa gagaataaag gaggaagact taggtaagtt gagccattga
     6241 gagcttttgg gggtaagtga taagacaatg acctcattcc tggctttgtg ctttacatgt
     6301 catagggctg aggagtatga caggtccact cgtattcatc caaagatgaa gatgagctct
     6361 atgggccatg caccagtggc ggattatttg tcatatcaga agcacaggca aatttaggta
     6421 acatcacttt gtccttctaa ctttagaatc tttattgcca tgtgtggtga gtagtagtcc
     6481 caactactca ggaggttcac tcgagcccag gaatttgaga ccagcctggg caatataacg
     6541 agatcttatc tcttaaaaaa aaaaaaaaag caaaagaaga agaaagagtc tatatctctt
     6601 gagtacagag attatatgcc cctagtacct agataggact ttgtgcgtgg taggtatcta
     6661 gacatttatg agacaataaa tgagttaggt tgatgttagc tttggaaaag aatcaaggat
     6721 aagtcttcag tgttttcttt aacagtacaa ttttagaatt ttgtttgttg gctttaggaa
     6781 atctcagtgg cattacatga attgcattat agcatgtact tagaaaaaag aacttatggc
     6841 tgggtgcggt ggctcacacc tgtaatccca gccttttggg aggccgaggt gggtggatca
     6901 cctgaagtca ggagttcaag accagcctga ccaacatggc gaaaccccat ctctactaaa
     6961 aatacaaaaa ttagccaggc atagtggcgg gcacctgtag tcccagctac tcaggaggct
     7021 gaggcaggag aatcacttga atctgggagg cggaggctgc agtgagccga gatcatgcca
     7081 ctgtactcca gcctgggcaa cagagtgaga ctctatctca aaaaaaaaaa aaaaaaaaaa
     7141 aaaaaaaaaa aacagaacct attaacctat tagacacttg aaaaaattcc tggcttgtag
     7201 ttgattatat gtttggttaa ttccatttca tctaaggaac taagtaattt aggaaatgtg
     7261 ttcttaacta ctcttagaag ggtacttgta gagaaccaga gtggtgattt tgtaagcagt
     7321 attttgtcct atacaatatg cactcttcca gtaactccag tagtccacat agctgactca
     7381 cctcctacaa cccgactttg ttaatatcaa gataactgtt ttataattgt agagaaagat
     7441 tttttacaac cagttgtagt tattattttc tttgcattat gttattgtga tagtagtggt
     7501 agctaatatg tgtaattaaa atgatcatct tatcctcaaa atcatcctat aaagtgcata
     7561 gaatccccat tttacagaag aggaaactga agctctcaat agggtagagg acttggctaa
     7621 ggtcacctag ctgctgagtg gcaaaactca gccttgaaca caggtcttag gactctcact
     7681 ttatatagct tggtaaaaga agatactaat taaatcctac ctaatagaga tgtatttaaa
     7741 attcactcac cccatctgtc ttacaggtaa tagttttaag gtaagataaa atcaaagtgt
     7801 tctaaaagta aatcataata cattttacag aattagagat aattgtgttt tagtggtcaa
     7861 acaatgctcg ttattctgtt agtaaaagca tggtaaaatg caaattaaaa taagatttct
     7921 cttttttttt cctgtcagat tgataaagat gaaaaagatt ggttctagta ttgacattgg
     7981 tatggggaaa tcattagtca ctgagatgat tagcaggact gtagattggt acaaatgaat
     8041 tggtgggcag ttcaacagga tctatcagac tttaaagtgt atctcatacc ctctgaaaca
     8101 gccatcctac tcttctatat ttattccatt ctctgtattc tactgaaaat ttagcacaaa
     8161 tatataaaca taagcatgca aggtattcat tgcagtattg cttgtaatag caaaaacaag
     8221 ccagcaaatc taaatgtctg ttaatagagg aatagataaa ttatggtacg tacatatagt
     8281 ggaatataat gtagccatta aaaagaatgc agatctgtat gtgatgattt acattgatta
     8341 ttcagtgtat gatcccactt gcattataaa tgtgtgcagt agttaaatat gagtgtgtgt
     8401 atatgtataa tacacactta cgtttgctag tatgggggag gagagcattg tttctcaaag
     8461 tgtggtctaa agacagcctg cattaggaaa tttttttttt aaatggggat tcctgggcat
     8521 tgtgctaatt tccctgaatc agaatcactg tgagtgaagc atggaaacct gtatttctaa
     8581 cgggttcctc agttgatggc ttatacaaat gtaaaatatg gtctagtagc tgtcagcaat
     8641 gaattggata tctggtagtg gattgtagtt agaaagggca aacttcttga agagatcttg
     8701 tattttaaat gaggcctacc taaaataaag ccagttgagt aacgttatca gcatagacct
     8761 gttaatgaag gtgaattggc ctgacataga aaaatgattc taccatacag cactacacac
     8821 caactaaatg agcaagcctt ttaaaaaatc cttgttgaac ttcccaaata tcagatgacc
     8881 tgttaataaa gcacaagtaa ataataggac ttactgtggt aagagaaagt gccaccttga
     8941 tttggttgtg tgtcagaacc agaaagtaag attatttgta gggctttggg atctatgctc
     9001 aaatggttta aggtatgtct gtcaaggcag ggaactgatt gggattagca aagttgatag
     9061 ttcagaatta gtggacacag caagcagggt tcttgaagat tcctgaaaag tcaactgttg
     9121 tttgagaaac aagctgtttg cccaggtgag cagggcttca cctgtaggaa ttgtcttcaa
     9181 ccttaggaat ttcctctaca aacaacaaca acaaaaaaat agggattgtt cttgttttta
     9241 tttgctttgt tttgtttgta atcttatctt tcctgggcaa agatttcctg gaataaacaa
     9301 ctaagttatg ttgacataca tgggctacag tcttatccaa gctgtattga cactgatgga
     9361 ttcagtttgt ttttcttagc tctctctatt caaagattat aagtcaatat tatgtatata
     9421 gcaattgaca ggtcataagt acggcatgct aactgatcgt accactggag ctccaacaat
     9481 tgatgtatct ttggtgactt cttagtgttc tgctgaagat cgcaaagatt tctgtagctt
     9541 cttgacctct ataacacttg gttcacactt gttaagtgtt ctaatataat ccacatcaca
     9601 ttgtgctgtg acttatcttt ctcatatttg tctccctcac tagaccacag ccctgtggga
     9661 atataatata actaatacat aatggtgaat aaagatttgt tttactagat agaatataaa
     9721 tcgaatccag gaaatcagtg gcagcatagg agagactgtg ttgcttctct tctattcaat
     9781 agaagtcttt gaaactcctt ttattctcta atattcttca tggagatgaa aatgaaggtt
     9841 gtaaagcttt actctgatta ttttaagcat gcctgcataa aaatgttttg agaagtaaat
     9901 tatgcatcat gtttctcttt agttagacta tgaatggtgt gaagaatttt ctgggctaat
     9961 gtttgacatt tattattcac tcctgtagat ttgtggtgaa tatgaatggt atctggacta
    10021 atcagaaaac aacgtaggaa aggcctaact tcaacactga attcttgctc cttgtctcac
    10081 ttgcttcctt ttgtagaaac tacaagtata tttaactttt gtatcctaga tccactacgg
    10141 cctactgaaa gcctatcatg catttatctt taaactttta ctctcttcat ttagccaaca
    10201 tatttctact tctatgttgc ctgctgtgct gtgtaatttt ctacaaacat tttcacctcc
    10261 aaatcccttg aaatgaaacc ctctggaaga gcatcaccac tttttcttcc tctattctaa
    10321 ttcccctcta gtttcccctc tagtttaaga cttttgtttc aatatttatt ctttccttaa
    10381 gcctttaaat tcatttatat ccaccaagtt gaatttgatg agacaggaca tgtttgaaag
    10441 aaggacaaac caaacaagca agcaaaccca tgaatactcg tacaatagga cataatatac
    10501 atttcccagt tccctgaagg ggtaaaattg agactggtta tacaagcagt attgcacagc
    10561 atgctgatac tagctagaca tagcctacag tgtttcagct tcactcaaag atggttctgg
    10621 aggacagtga ggaaatgaac tttttccagc gagaataaat atgagcagta tttctagtct
    10681 gctttgcacc gaaagtgagc catcccaaga tacagatcta caccatttat acctggccag
    10741 gtggttggaa acacagacgt aataagattg gaggatgagt gacaaaaatt gggagctaaa
    10801 catattggac ttcttaaaat aggctcagat tgcaagaata attgtttcct acagacatat
    10861 taaacaaagg ccctgtggtg aaggaggtgc taaataatca gatggtcaag atgactttga
    10921 ggatatcagt aaatctcttt ggtattccag cctttgttca aacagtttat gggcaaagcg
    10981 gctatactgg aaggataggg ctacacagga acttcaacaa tacaggcctc ctttcatcaa
    11041 ggctgtcctt attactgcca ggagccgaac tggccagcag cagtgaccat cctaagatac
    11101 aacatctgag ggtgaccaga tagttagtgg cagattgatt atatcaaata ttttctttca
    11161 tggagagaca actatttgtc ctcatggaat agagccttac tctggatctg gctttaagtt
    11221 tgccttccct aagcaccatg cttgtgtgtg caataataag cttcctgttc accattatga
    11281 ttttccccaa aatattgctt ctaagaaact tattttacaa tgtaatcagt gaggtagagg
    11341 tagaattaca ctttcagtca tgggaaacta ggtaattaac atcaaaattc ccattggcaa
    11401 ctagataatc cagcaaaact cttcaaaaca tgaaaatcta ggccaggcgc agtggcttat
    11461 gcctataatc ccagcacttt gggaagccga ggtgggtgga tcacctaaag tcagaagttc
    11521 aagaccagcc tggccaacat ggtgaaagcc cgtctctact aaaaatacaa aaaaaattag
    11581 ccaggtctgg tagcacatgc ctgtaatccc agctactcag gaggctgtgg caggagaatc
    11641 gcttgaacct gagaagcgga ggttgaggtg agccgagata gcaccactgc actccagcct
    11701 gggcaacaga ggaagactct gtctctaaat aaataaacat gaaaatctag agaggcaagc
    11761 attggcacaa cttctttcct ggaggtattg gctgattcta gaagcattta aggggataag
    11821 cagtgcttct tactgctcca ggatattagg aggacaaaaa ctgaatgcca gtgcctgcca
    11881 agtcataaaa gagggcttag gaaattccca tgctttgggc tgggacccaa aaaagctcca
    11941 ccataggagt aagagtgaat tggaagtgca tcaactctcc cccatacaca gggaattcag
    12001 cacggattca aaccatccca attcctgaaa tcagattaaa aatgatcccc taagtgcttg
    12061 gtaataaaca aatcctctgt ggaggtggaa aaaaaacccc atcatcttaa gcctcaagtt
    12121 ctacaatttt tcaaatacag tcttcacaag agaaaagaaa aataaccaga cacttctata
    12181 gcagagcaac actcattgag aacaacaagc agctttgggt aaaattgctt aaaacctctg
    12241 tttgaagcca ttgaagaact ttcagctaaa caggactcta gaagacaaaa cgccagagag
    12301 aaggtgtgca gaaaagagtt aacataagag gctgagatgc tatccttagc cctgatttta
    12361 aagctgggct ttggctagtg tctgggaact tggattaccg gaatgttccc accattccct
    12421 aactgataag ggggtttact ctttgtaaac tatataaaca acatggttta tgctgaattc
    12481 ccgctttgct tctaggagtc tggaattatg atttgtgctg ggcagagggt gcctctatca
    12541 ctaacttcca atacaaactt tgggcagtaa gactctaacg agcatccctg gcagacaaca
    12601 tttcacacct gttgtcacaa ttcagtactg aagtaattaa ccacagcctg tgtgattcca
    12661 caggcaaagg attcttggaa tcttgtgtca ggtttcctct ggacttcacc ccatgtcctt
    12721 ttcccctttg ctgatttgct ttgtagtact tttctctaat aaatcatagc catgagtatg
    12781 actatatgct gagccttgtg agtgcttctg ttgaatcact gaacctgggg tgctggcgtg
    12841 gtcttgggga ctcccaatac aaaagtattt taaccagaga agtgagcaga cattctgcag
    12901 ctacttcttc cattatggaa tttgccaaat ctgggtaaaa ggcccaagaa gagagttact
    12961 gttaagaatc agagaagcaa acaagttatg gaaaccacac aggactggag aaacaaaaag
    13021 aacagggcct ttaaaccaat cgcacttggg agagcagaga tcctagtaag acaggaagtt
    13081 cagagaagtg agcctggcac tttccttgtt ttcctcttga gtatttgctg aattcttgga
    13141 ttgctcagag agggaaaaaa aaaaaaaaaa aaaaaaaa
//
