LOCUS       NM_012269               2410 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens hyaluronidase 4 (HYAL4), mRNA.
ACCESSION   NM_012269
VERSION     NM_012269.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Lokeshwar,V.B., Morera,D.S., Hasanali,S.L., Yates,T.J., Hupe,M.C.,
            Knapp,J., Lokeshwar,S.D., Wang,J., Hennig,M.J.P., Baskar,R.,
            Escudero,D.O., Racine,R.R., Dhir,N., Jordan,A.R., Hoye,K., Azih,I.,
            Manoharan,M., Klaassen,Z., Kavuri,S., Lopez,L.E., Ghosh,S. and
            Lokeshwar,B.L.
  TITLE     A Novel Splice Variant of HYAL-4 Drives Malignant Transformation
            and Predicts Outcome in Patients with Bladder Cancer
  JOURNAL   Clin Cancer Res 26 (13), 3455-3467 (2020)
   PUBMED   32094233
  REMARK    GeneRIF: A Novel Splice Variant of HYAL-4 Drives Malignant
            Transformation and Predicts Outcome in Patients with Bladder
            Cancer.
REFERENCE   3  (bases 1 to 2410)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 2410)
  AUTHORS   Kaneiwa,T., Miyazaki,A., Kogawa,R., Mizumoto,S., Sugahara,K. and
            Yamada,S.
  TITLE     Identification of amino acid residues required for the substrate
            specificity of human and mouse chondroitin sulfate hydrolase
            (conventional hyaluronidase-4)
  JOURNAL   J Biol Chem 287 (50), 42119-42128 (2012)
   PUBMED   23086929
  REMARK    GeneRIF: Identification of amino acid residues required for the
            substrate specificity of human and mouse chondroitin sulfate
            hydrolase (conventional hyaluronidase-4).
REFERENCE   5  (bases 1 to 2410)
  AUTHORS   Kaneiwa,T., Mizumoto,S., Sugahara,K. and Yamada,S.
  TITLE     Identification of human hyaluronidase-4 as a novel chondroitin
            sulfate hydrolase that preferentially cleaves the galactosaminidic
            linkage in the trisulfated tetrasaccharide sequence
  JOURNAL   Glycobiology 20 (3), 300-309 (2010)
   PUBMED   19889881
  REMARK    GeneRIF: Identification of human hyaluronidase-4 as a novel
            chondroitin sulfate hydrolase that preferentially cleaves the
            galactosaminidic linkage in the trisulfated tetrasaccharide
            sequence
REFERENCE   6  (bases 1 to 2410)
  AUTHORS   Fiszer-Szafarz,B., Litynska,A. and Zou,L.
  TITLE     Human hyaluronidases: electrophoretic multiple forms in somatic
            tissues and body fluids. Evidence for conserved hyaluronidase
            potential N-glycosylation sites in different mammalian species
  JOURNAL   J Biochem Biophys Methods 45 (2), 103-116 (2000)
   PUBMED   10989127
REFERENCE   7  (bases 1 to 2410)
  AUTHORS   Csoka,A.B., Scherer,S.W. and Stern,R.
  TITLE     Expression analysis of six paralogous human hyaluronidase genes
            clustered on chromosomes 3p21 and 7q31
  JOURNAL   Genomics 60 (3), 356-361 (1999)
   PUBMED   10493834
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC006029.2, BG013757.1,
            BF085228.1, BC104788.1 and AF009010.1.
            
            On May 17, 2019 this sequence version replaced NM_012269.2.
            
            Summary: This gene encodes a protein which is similar in structure
            to hyaluronidases but lacks hyaluronidase activity. The encoded
            protein acts as a chondroitin-sulfate-specific
            endo-beta-N-acetylgalactosaminidase; that is, it exhibits
            hydrolytic activity toward chondroitin sulfate chains and degrades
            them into oligosaccharides. Proteoglycans are formed by the
            covalent linkage of chondroitin sulfate chains to protein.
            Proteoglycans are ubiquitous components of the extracellular matrix
            of connective tissues and are also found at the surface of many
            cell types where they participate in a variety of cellular
            processes such as cell proliferation, differentiation, migration,
            cell-cell recognition, extracellular matrix deposition, and tissue
            morphogenesis. The expression of this gene is highest in testes and
            placenta. [provided by RefSeq, Apr 2019].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            CDS exon combination :: AB470346.1, BC104790.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMEA1968968, SAMEA2148093 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000223026.9/ ENSP00000223026.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-503               AC006029.2         92630-93132
            504-600             BG013757.1         47-143
            601-802             BF085228.1         149-350             c
            803-1673            BC104788.1         241-1111
            1674-2407           AF009010.1         1677-2410
            2408-2410           AC006029.2         124940-124942
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q31.32"
     gene            1..2410
                     /gene="HYAL4"
                     /gene_synonym="CSHY; HYAL-4"
                     /note="hyaluronidase 4"
                     /db_xref="GeneID:23553"
                     /db_xref="HGNC:HGNC:5323"
                     /db_xref="MIM:604510"
     exon            1..517
                     /gene="HYAL4"
                     /gene_synonym="CSHY; HYAL-4"
                     /inference="alignment:Splign:2.1.0"
     exon            518..587
                     /gene="HYAL4"
                     /gene_synonym="CSHY; HYAL-4"
                     /inference="alignment:Splign:2.1.0"
     exon            588..1592
                     /gene="HYAL4"
                     /gene_synonym="CSHY; HYAL-4"
                     /inference="alignment:Splign:2.1.0"
     CDS             639..2084
                     /gene="HYAL4"
                     /gene_synonym="CSHY; HYAL-4"
                     /EC_number="3.2.1.35"
                     /note="chondroitin sulfate hydrolase; chondroitin sulfate
                     endo-beta-N-acetylgalactosaminidase;
                     hyaluronoglucosaminidase 4"
                     /codon_start=1
                     /product="hyaluronidase-4"
                     /protein_id="NP_036401.2"
                     /db_xref="CCDS:CCDS5789.1"
                     /db_xref="GeneID:23553"
                     /db_xref="HGNC:HGNC:5323"
                     /db_xref="MIM:604510"
                     /translation="MKVLSEGQLKLCVVQPVHLTSWLLIFFILKSISCLKPARLPIYQ
                     RKPFIAAWNAPTDQCLIKYNLRLNLKMFPVIGSPLAKARGQNVTIFYVNRLGYYPWYT
                     SQGVPINGGLPQNISLQVHLEKADQDINYYIPAEDFSGLAVIDWEYWRPQWARNWNSK
                     DVYRQKSRKLISDMGKNVSATDIEYLAKVTFEESAKAFMKETIKLGIKSRPKGLWGYY
                     LYPDCHNYNVYAPNYSGSCPEDEVLRNNELSWLWNSSAALYPSIGVWKSLGDSENILR
                     FSKFRVHESMRISTMTSHDYALPVFVYTRLGYRDEPLFFLSKQDLVSTIGESAALGAA
                     GIVIWGDMNLTASKANCTKVKQFVSSDLGSYIANVTRAAEVCSLHLCRNNGRCIRKMW
                     NAPSYLHLNPASYHIEASEDGEFTVKGKASDTDLAVMADTFSCHCYQGYEGADCREIK
                     TADGCSGVSPSPGSLMTLCLLLLASYRSIQL"
     exon            1593..1682
                     /gene="HYAL4"
                     /gene_synonym="CSHY; HYAL-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..2410
                     /gene="HYAL4"
                     /gene_synonym="CSHY; HYAL-4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2388..2393
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HYAL4"
                     /gene_synonym="CSHY; HYAL-4"
                     /note="hexamer: ATTAAA"
     polyA_site      2410
                     /gene="HYAL4"
                     /gene_synonym="CSHY; HYAL-4"
                     /note="major polyA site"
ORIGIN      
        1 tctttatttt atttatgcta tctatttctt ttcctttttt tttttttttt ttttttttga
       61 gatgaagtct tactctgttg cccaggctag agtgtagtgg tgtgatctcg gctcactgca
      121 accactgcct cctgggttca agtgattctc ctgacttaac ctcctgagta gctgggacta
      181 caagagcatg ccatcatgcc cagctaattt ttgtattttt agtagagatg gggtttcacc
      241 atattggcca gaatggtttt gcattcctga cctcaagtga tctgcctgcc tcagcctccc
      301 aaaatgttgg gttacagggg tgagccaccg tgccttgcta tttatgccat ctatttcact
      361 gaagattccg cctctcattt cttgagtcat tttttttaaa tttccttaaa ttggacttca
      421 catttttctg atgcctcctt gtttagctta ataactgacc ttctgaattc ttttttagga
      481 aaatcaggaa tttcttcttg gtttggagcc attgctggac atcctttgcc attcaacctc
      541 tgatttgcac aaggtgacta aaggaccagc agcaaacaaa agcaaaggtc ttctagagtg
      601 cactaaagca gaagataacg taacattttt atcttaccat gaaagtatta tctgaaggac
      661 agttaaagct ttgtgttgtt caaccagtac atctcacttc atggctcctt atatttttta
      721 ttctaaagtc tatctcttgt ctaaaacctg ctcgacttcc aatttatcaa aggaaacctt
      781 ttatagctgc ttggaatgct ccaacagatc agtgtttgat aaaatataat ttaagactaa
      841 atttgaaaat gtttcctgtg attggaagcc cactggccaa ggccaggggg caaaatgtca
      901 ctatatttta tgtcaacaga ttgggatact atccgtggta tacatcacaa ggggtcccca
      961 ttaatggagg tctcccacag aacataagtt tacaagtaca tctggaaaaa gctgaccaag
     1021 atattaatta ttacatccct gctgaagatt tcagtggact tgctgttata gattgggaat
     1081 attggcgacc acagtgggcc cggaactgga actcaaaaga tgtttacaga cagaagtcaa
     1141 gaaagcttat ttccgatatg ggaaagaatg tatcagctac cgatattgaa tatttagcca
     1201 aagtgacctt tgaagaaagt gcaaaagctt tcatgaagga aaccatcaaa ttgggaatta
     1261 agagccgacc caaaggcctt tggggttatt atttatatcc tgattgccac aattataacg
     1321 tttatgcccc aaactactct gggtcatgcc cagaagacga agtcttgagg aacaatgagc
     1381 tctcttggct ctggaacagc agtgctgctt tatatccttc tatcggtgtc tggaaatccc
     1441 ttggagacag tgaaaacatt ttgcgcttct ccaaatttcg ggtgcatgaa tccatgagga
     1501 tctccaccat gacatctcat gattatgctc tgcctgtatt tgtctacaca aggctagggt
     1561 acagagatga acctttattt ttcctttcta agcaagatct agtcagcacc ataggagaaa
     1621 gtgctgcctt gggagctgca ggcattgtta tttggggaga catgaattta actgcatcca
     1681 aggccaactg tacaaaggtg aagcagtttg tgagttctga tttagggagc tacatagcca
     1741 atgtgaccag agctgctgag gtatgcagcc ttcacctctg caggaacaat ggcaggtgca
     1801 taaggaagat gtggaacgcg cccagttacc ttcacttgaa ccctgcaagt taccacatag
     1861 aggcctctga ggacggggag tttactgtga aaggaaaagc atctgataca gacctggcag
     1921 tgatggcaga tacattttcc tgtcattgtt atcagggata tgaaggagct gattgcagag
     1981 aaataaagac ggctgatggc tgctctgggg tttccccttc tcctggttca ctaatgacac
     2041 tttgtctact gcttttagca agttatcgaa gcattcagtt gtgagataat tgagtttaaa
     2101 gggaattgtg tggcctctag cctagtcatt taaagaagga tgtaacttat aacatttttt
     2161 ttctcttatg aattctattg agagatatta taagtagaca ttatgtatgt cacttaacat
     2221 aaacagaaac attattttat ttgcctccag tctggctagg aaaccagatc tggggtaaag
     2281 tcaatgtaca cttcctcctt attggaatat ttaagttgca tttaaactaa aactagtata
     2341 atttagtctt ttcatgaatg tacatacata aaattataca taaaaatatt aaattattca
     2401 tttcaaagac
//
