LOCUS       NM_004507               3007 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens HUS1 checkpoint clamp component (HUS1), transcript
            variant 1, mRNA.
ACCESSION   NM_004507
VERSION     NM_004507.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Hara,K., Hishiki,A., Hoshino,T., Nagata,K., Iida,N., Sawada,Y.,
            Ohashi,E. and Hashimoto,H.
  TITLE     The 9-1-1 DNA clamp subunit RAD1 forms specific interactions with
            clamp loader RAD17, revealing functional implications for
            binding-protein RHINO
  JOURNAL   J Biol Chem 299 (4), 103061 (2023)
   PUBMED   36841485
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 3007)
  AUTHORS   Ren,K., Yu,Y., Wang,X., Liu,H. and Zhao,J.
  TITLE     MiR-340-3p-HUS1 axis suppresses proliferation and migration in lung
            adenocarcinoma cells
  JOURNAL   Life Sci 274, 119330 (2021)
   PUBMED   33711383
  REMARK    GeneRIF: MiR-340-3p-HUS1 axis suppresses proliferation and
            migration in lung adenocarcinoma cells.
REFERENCE   4  (bases 1 to 3007)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3007)
  AUTHORS   Hong,H., Yao,S., Zhang,Y., Ye,Y., Li,C., Hu,L., Sun,Y., Huang,H.Y.
            and Ji,H.
  TITLE     In vivo miRNA knockout screening identifies miR-190b as a novel
            tumor suppressor
  JOURNAL   PLoS Genet 16 (11), e1009168 (2020)
   PUBMED   33137086
  REMARK    GeneRIF: In vivo miRNA knockout screening identifies miR-190b as a
            novel tumor suppressor.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3007)
  AUTHORS   St Onge,R.P., Udell,C.M., Casselman,R. and Davey,S.
  TITLE     The human G2 checkpoint control protein hRAD9 is a nuclear
            phosphoprotein that forms complexes with hRAD1 and hHUS1
  JOURNAL   Mol Biol Cell 10 (6), 1985-1995 (1999)
   PUBMED   10359610
REFERENCE   7  (bases 1 to 3007)
  AUTHORS   Volkmer,E. and Karnitz,L.M.
  TITLE     Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9
            form a DNA damage-responsive protein complex
  JOURNAL   J Biol Chem 274 (2), 567-570 (1999)
   PUBMED   9872989
REFERENCE   8  (bases 1 to 3007)
  AUTHORS   Dean,F.B., Lian,L. and O'Donnell,M.
  TITLE     cDNA cloning and gene mapping of human homologs for
            Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of
            homologs from mouse, Caenorhabditis elegans, and Drosophila
            melanogaster
  JOURNAL   Genomics 54 (3), 424-436 (1998)
   PUBMED   9878245
REFERENCE   9  (bases 1 to 3007)
  AUTHORS   Dominguez,O., Ashhab,Y., Sabater,L., Belloso,E., Caro,P. and
            Pujol-Borrell,R.
  TITLE     Cloning of ARE-containing genes by AU-motif-directed display
  JOURNAL   Genomics 54 (2), 278-286 (1998)
   PUBMED   9828130
REFERENCE   10 (bases 1 to 3007)
  AUTHORS   Kostrub,C.F., Knudsen,K., Subramani,S. and Enoch,T.
  TITLE     Hus1p, a conserved fission yeast checkpoint protein, interacts with
            Rad1p and is phosphorylated in response to DNA damage
  JOURNAL   EMBO J 17 (7), 2055-2066 (1998)
   PUBMED   9524127
  REMARK    GeneRIF: Functional study of the yeast homolog.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG286955.1, BC007013.1,
            AC069282.6 and CA438762.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004507.3.
            
            Summary: The protein encoded by this gene is a component of an
            evolutionarily conserved, genotoxin-activated checkpoint complex
            that is involved in the cell cycle arrest in response to DNA
            damage. This protein forms a heterotrimeric complex with checkpoint
            proteins RAD9 and RAD1. In response to DNA damage, the trimeric
            complex interacts with another protein complex consisting of
            checkpoint protein RAD17 and four small subunits of the replication
            factor C (RFC), which loads the combined complex onto the
            chromatin. The DNA damage induced chromatin binding has been shown
            to depend on the activation of the checkpoint kinase ATM, and is
            thought to be an early checkpoint signaling event. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Feb 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes a protein of 280 aa.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.198252.1,
                                           SRR1803616.145768.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000258774.10/ ENSP00000258774.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                BG286955.1         39-110
            73-1131             BC007013.1         1-1059
            1132-2909           AC069282.6         152273-154050       c
            2910-3007           CA438762.1         17-114              c
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p12.3"
     gene            1..3007
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /note="HUS1 checkpoint clamp component"
                     /db_xref="GeneID:3364"
                     /db_xref="HGNC:HGNC:5309"
                     /db_xref="MIM:603760"
     exon            1..148
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..939
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     hus1+-like protein; checkpoint protein HUS1; HUS1
                     checkpoint homolog"
                     /codon_start=1
                     /product="checkpoint protein HUS1 isoform 1"
                     /protein_id="NP_004498.1"
                     /db_xref="CCDS:CCDS34635.1"
                     /db_xref="GeneID:3364"
                     /db_xref="HGNC:HGNC:5309"
                     /db_xref="MIM:603760"
                     /translation="MKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILC
                     DKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALK
                     IKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYL
                     PVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASEST
                     HEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFI
                     PALS"
     exon            149..276
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /inference="alignment:Splign:2.1.0"
     exon            277..453
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /inference="alignment:Splign:2.1.0"
     exon            454..561
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /inference="alignment:Splign:2.1.0"
     exon            562..636
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /inference="alignment:Splign:2.1.0"
     exon            637..736
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /inference="alignment:Splign:2.1.0"
     exon            737..856
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /inference="alignment:Splign:2.1.0"
     exon            857..3007
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1110..1115
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /note="hexamer: GATAAA"
     polyA_site      1132
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /note="major polyA site"
     regulatory      2919..2924
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /note="hexamer: AATACA"
     polyA_site      2944
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /note="major polyA site"
     regulatory      2985..2990
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /note="hexamer: AAGAAA"
     polyA_site      3007
                     /gene="HUS1"
                     /gene_synonym="hHUS1"
                     /note="major polyA site"
ORIGIN      
        1 actgttcgcg ctgccgtgtt tccgggcggg gacactcagg gcgcgacgct tttctgttac
       61 ccacagaggc ccgccgcggc tgcgccatcc gcggccatga agtttcgggc caagatcgtg
      121 gacggggcct gtctgaacca cttcacacga atcagtaaca tgatagccaa gcttgccaaa
      181 acctgcaccc tccgcatcag ccctgataag cttaacttca tcctttgtga caagctggct
      241 aatggaggag tgagcatgtg gtgtgagctg gaacaggaga acttcttcaa cgaatttcaa
      301 atggagggtg tctctgcaga aaacaatgag atttatttag agctaacatc ggaaaactta
      361 tctcgagcct tgaagactgc ccagaatgcc agggctttga aaatcaaact gactaataaa
      421 cactttccct gcctcacggt ctccgtggag ctgttatcta tgtcaagcag tagccgcatt
      481 gtgacccatg acatccccat aaaggtgatt cctaggaaat tgtggaagga cttacaagaa
      541 ccggtggtcc cagatcctga tgttagtatt tatttaccag tcttgaagac tatgaagagt
      601 gttgtggaaa aaatgaaaaa catcagcaat caccttgtta ttgaagcaaa cctagatgga
      661 gaattgaatt tgaaaataga aactgaatta gtatgtgtta caactcattt taaagatctt
      721 ggaaatcctc cattagcctc tgaaagcacc catgaggaca gaaacgtgga acacatggct
      781 gaagtgcaca tagatattag gaagctccta cagtttcttg ctggacaaca agtaaatccc
      841 acaaaggcct tatgcaatat tgtgaataac aagatggtgc attttgatct gcttcatgaa
      901 gacgtgtccc ttcagtattt catccctgcg ctgtcctagc accctgtcgc tggagttggc
      961 atgcagagac tttgtcagga tgggagaggc cgcaggtgtt gtgttctgat cactggtctg
     1021 tgccctcaca gcaccgcaca tcgacacact gtacttattt gtccctctct aacattttaa
     1081 ctaaaagttg attcaacaac acacagttgg ataaacatat cacttcatgt tgctcatgtc
     1141 tgttttgctt tgtttttaag acactgaaaa gaaaagctag aatttattta ttcagacttt
     1201 aaagaacaat ttctcattga tgttgtgaaa atcgtcatgt atttagactt ggtgtagtag
     1261 ccagaattcg taaagctgtt gcctgggagc ttggtacttt ccctccaggc agaggctcta
     1321 gctcagcacg gcctgtagcg cacagtcagt cttgcatttc agtgtgttca ccccgctgct
     1381 cctgcccctt ggagcccagt gacagaaaga acagcctctg tcaccccgcc gccactgcct
     1441 tggttactca gagcactgtg gggtgtcaca gctgcagcat ttggagtctc tctcttgctg
     1501 aggactcaag cccacctgag tccactcccc tcttgatgcc tagagagctg gcccagccaa
     1561 cacagctctt agctgggagc tccttctgcc attccaacta gtttcttcct ggggccagtt
     1621 ttgggtttag gttgtaattc cttatatttc tttcttccac agtgtatcgg atctgtcgtt
     1681 ctggaaagaa gacccttcta tttagagtag aaacaaacga aacttctaag gtatcatctg
     1741 tgttaagtga tgagaccata tttctttgat gtttctgaac atcaaagctg attcagtact
     1801 ggtagatgtg ctcattctcc ctgaaacata cccatcatat ttcctattat aattacatct
     1861 cattgtcctg tggaggtgga catgataaac attatctttt gttttcttgt tttgttttgt
     1921 ttgagacggt ctcattctgt cacccagact ggagtgcagt gccacaatca tggctcaccg
     1981 cattgacctc cttggctcaa ggcatcctcc cacctcagct tcctgactag ctgggactac
     2041 tggtgtgcac caccacaccc agctaatttt caatttttca tagagacagg gtctcactgt
     2101 gttgtccagg ctggtcttga actcctgggc tcaagccacc cgcccacctg ggcctcccaa
     2161 agtgctggga ttacaggcat cagccatcac acccatccat aaacattata ttaatgtcac
     2221 attacaaaac tgagacctaa gttgcttagg ataaaatgaa attggaagac tagctaacat
     2281 gaaaatttat attttggctt tttcatgttt tttgataaaa ccagtgtatt tgaatgattc
     2341 ttttgatgtt tagtaatggt tttttgtttg ttttttggtt tttttttttg agacggagtc
     2401 tcactctgtc gccagtctgg agtgctagtg gcgcgatctt ggcttactgc agcctccact
     2461 tcccaagttc aagcgattct cctgcctcag cctcccgagt agctgggact acaggcgcat
     2521 gccaccacgc ccggctaatt tttgtatttt tagtagagac ggagtttcac tgtgttggcc
     2581 aggatggtct cgatctcgac ctcgtgatca cccaccttgg cctctcaaag tgctgggatt
     2641 acaggcgtga gccaccacgc ctggcctatg tttaataatg ttgaaatagg atggaatatt
     2701 ttgttaaatt aacattttaa aattagaaga caccgtttta atttttaaac ccttcctcct
     2761 ctcattgtaa cgaaattaat tccagctgca gtgagaaaac ttaaaaatca tgatacaaaa
     2821 tgaaacaata tctgaaagta gttttataaa actgaaattg ctgttaaaga gaatgtgtta
     2881 gtgacttaac catttgctct atgtgatgtt tattatcaaa tacatataat tttgaagatt
     2941 ttaatgaatg gcttaagatt ttatctttgt gtagaatgtg gctaaagaaa ccttagttga
     3001 gattcaa
//
