LOCUS       NM_153692               2032 bp    mRNA    linear   PRI 09-JUL-2024
DEFINITION  Homo sapiens HtrA serine peptidase 4 (HTRA4), mRNA.
ACCESSION   NM_153692
VERSION     NM_153692.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Donohoe,M.E., Morey,R., Li,Y., Pizzo,D., Kallol,S., Cho,H.Y.,
            Soncin,F. and Parast,M.M.
  TITLE     Identification of HTRA4 as a Transcriptional Target of p63 in
            Trophoblast
  JOURNAL   Am J Pathol 194 (7), 1162-1170 (2024)
   PUBMED   38880601
  REMARK    GeneRIF: Identification of HTRA4 as a Transcriptional Target of p63
            in Trophoblast.
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Thach,B., Wang,Y., Heng,S. and Nie,G.
  TITLE     HtrA4 is required for human trophoblast stem cell differentiation
            into syncytiotrophoblast
  JOURNAL   Placenta 147, 68-77 (2024)
   PUBMED   38325051
  REMARK    GeneRIF: HtrA4 is required for human trophoblast stem cell
            differentiation into syncytiotrophoblast.
REFERENCE   3  (bases 1 to 2032)
  AUTHORS   Pei,C.Z., Seok,J., Kim,G.J., Choi,B.C. and Baek,K.H.
  TITLE     Deficiency of HtrA4 in BeWo cells downregulates angiogenesis
            through IL-6/JAK/STAT3 signaling
  JOURNAL   Biomed Pharmacother 166, 115288 (2023)
   PUBMED   37579694
  REMARK    GeneRIF: Deficiency of HtrA4 in BeWo cells downregulates
            angiogenesis through IL-6/JAK/STAT3 signaling.
REFERENCE   4  (bases 1 to 2032)
  AUTHORS   Pei,C.Z., Choi,B.C., Park,J.H., Park,H.Y., Paek,J., Lee,K.J.,
            Yun,B.S., Kim,Y.J. and Baek,K.H.
  TITLE     Cellular Functions of High-Temperature Requirement Factor A4 in
            Placenta
  JOURNAL   Cells 12 (11), 1459 (2023)
   PUBMED   37296580
  REMARK    GeneRIF: Cellular Functions of High-Temperature Requirement Factor
            A4 in Placenta.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2032)
  AUTHORS   Mansilla,M., Wang,Y., Lim,R., Palmer,K. and Nie,G.
  TITLE     HtrA4 is up-regulated during trophoblast syncytialization and BeWo
            cells fail to syncytialize without HtrA4
  JOURNAL   Sci Rep 11 (1), 14363 (2021)
   PUBMED   34257367
  REMARK    GeneRIF: HtrA4 is up-regulated during trophoblast syncytialization
            and BeWo cells fail to syncytialize without HtrA4.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2032)
  AUTHORS   Chen,Y.Y., Chuang,P.Y., Chen,C.P., Chiu,Y.H., Lo,H.F., Cheong,M.L.,
            Huang,J.Y., Kuo,P.L. and Chen,H.
  TITLE     Functional antagonism between high temperature requirement protein
            A (HtrA) family members regulates trophoblast invasion
  JOURNAL   J Biol Chem 289 (33), 22958-22968 (2014)
   PUBMED   25002585
  REMARK    GeneRIF: Conditioned media from these two cell lines after
            decidualization treatment suppress HtrA4-expressing JAR cell
            invasion in an HtrA1- or HtrA3-dependent manner.
REFERENCE   7  (bases 1 to 2032)
  AUTHORS   Inagaki,A., Nishizawa,H., Ota,S., Suzuki,M., Inuzuka,H.,
            Miyamura,H., Sekiya,T., Kurahashi,H. and Udagawa,Y.
  TITLE     Upregulation of HtrA4 in the placentas of patients with severe
            pre-eclampsia
  JOURNAL   Placenta 33 (11), 919-926 (2012)
   PUBMED   22964307
  REMARK    GeneRIF: Data suggest that increased HtrA4 (and HtrA1) in placenta
            tissues may be involved in onset of pre-eclampsia; elevated level
            of HtrA4 (but not HtrA1) in sera has potential as biomarker for
            pre-eclampsia.
REFERENCE   8  (bases 1 to 2032)
  AUTHORS   Wang,L.J., Cheong,M.L., Lee,Y.S., Lee,M.T. and Chen,H.
  TITLE     High-temperature requirement protein A4 (HtrA4) suppresses the
            fusogenic activity of syncytin-1 and promotes trophoblast invasion
  JOURNAL   Mol Cell Biol 32 (18), 3707-3717 (2012)
   PUBMED   22778138
  REMARK    GeneRIF: study reveals a novel function of GCM1 and HtrA4 in
            regulation of trophoblast invasion and that abnormal HrtA4
            expression may contribute to shallow trophoblast invasion in
            preeclampsia
REFERENCE   9  (bases 1 to 2032)
  AUTHORS   Ketteler,R., Sun,Z., Kovacs,K.F., He,W.W. and Seed,B.
  TITLE     A pathway sensor for genome-wide screens of intracellular
            proteolytic cleavage
  JOURNAL   Genome Biol 9 (4), R64 (2008)
   PUBMED   18387192
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 2032)
  AUTHORS   Clausen,T., Southan,C. and Ehrmann,M.
  TITLE     The HtrA family of proteases: implications for protein composition
            and cell fate
  JOURNAL   Mol Cell 10 (3), 443-455 (2002)
   PUBMED   12408815
  REMARK    GeneRIF: FLJ90724 or HTRA4 is a member of the HtrA family of
            proteases
            Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK075205.1, AC108863.4 and
            AY188774.1.
            
            On Nov 23, 2018 this sequence version replaced NM_153692.3.
            
            Summary: This gene encodes a member of the HtrA family of
            proteases. The encoded protein contains a putative signal peptide,
            an insulin growth factor binding domain, a Kazal protease inhibitor
            domain, a conserved trypsin domain and a PDZ domain. Based on
            studies on other related family members, this enzyme may function
            as a secreted oligomeric chaperone protease to degrade misfolded
            secretory proteins. Other human HtrA proteins have been implicated
            in arthritis, tumor suppression, unfolded stress response,
            apoptosis, and aging. [provided by RefSeq, Oct 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057765.1, AK075205.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA2142853
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000302495.5/ ENSP00000305919.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1001              AK075205.1         51-1051
            1002-1002           AC108863.4         83048-83048
            1003-1035           AC108863.4         84116-84148
            1036-1150           AC108863.4         86554-86668
            1151-1208           AC108863.4         87399-87456
            1209-1304           AC108863.4         87854-87949
            1305-1561           AC108863.4         92837-93093
            1562-2031           AY188774.1         160-629
            2032-2032           AC108863.4         93564-93564
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p11.22"
     gene            1..2032
                     /gene="HTRA4"
                     /note="HtrA serine peptidase 4"
                     /db_xref="GeneID:203100"
                     /db_xref="HGNC:HGNC:26909"
                     /db_xref="MIM:610700"
     exon            1..502
                     /gene="HTRA4"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..1467
                     /gene="HTRA4"
                     /note="probable serine protease HTRA4; high-temperature
                     requirement factor A4"
                     /codon_start=1
                     /product="serine protease HTRA4 precursor"
                     /protein_id="NP_710159.1"
                     /db_xref="CCDS:CCDS6110.1"
                     /db_xref="GeneID:203100"
                     /db_xref="HGNC:HGNC:26909"
                     /db_xref="MIM:610700"
                     /translation="MIRPQLRTAGLGRCLLPGLLLLLVPVLWAGAEKLHTQPSCPAVC
                     QPTRCPALPTCALGTTPVFDLCRCCRVCPAAEREVCGGAQGQPCAPGLQCLQPLRPGF
                     PSTCGCPTLGGAVCGSDRRTYPSMCALRAENRAARRLGKVPAVPVQWGNCGDTGTRSA
                     GPLRRNYNFIAAVVEKVAPSVVHVQLWGRLLHGSRLVPVYSGSGFIVSEDGLIITNAH
                     VVRNQQWIEVVLQNGARYEAVVKDIDLKLDLAVIKIESNAELPVLMLGRSSDLRAGEF
                     VVALGSPFSLQNTATAGIVSTKQRGGKELGMKDSDMDYVQIDATINYGNSGGPLVNLD
                     GDVIGVNSLRVTDGISFAIPSDRVRQFLAEYHEHQMKGKAFSNKKYLGLQMLSLTVPL
                     SEELKMHYPDFPDVSSGVYVCKVVEGTAAQSSGLRDHDVIVNINGKPITTTTDVVKAL
                     DSDSLSMAVLRGKDNLLLTVIPETIN"
     exon            503..602
                     /gene="HTRA4"
                     /inference="alignment:Splign:2.1.0"
     exon            603..807
                     /gene="HTRA4"
                     /inference="alignment:Splign:2.1.0"
     exon            808..1002
                     /gene="HTRA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1035
                     /gene="HTRA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1150
                     /gene="HTRA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1208
                     /gene="HTRA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1304
                     /gene="HTRA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..2032
                     /gene="HTRA4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2010..2015
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HTRA4"
                     /note="hexamer: AATAAA"
     polyA_site      2029
                     /gene="HTRA4"
                     /note="major polyA site"
ORIGIN      
        1 aaagtcactg aagagtggaa gcgaggaagg aacaggatga ttagacctca gctgcggacc
       61 gcggggctgg gacgatgcct cctgccgggg ctgctgctgc tcctggtgcc cgtcctctgg
      121 gccggggctg aaaagctaca tacccagccc tcctgccccg cggtctgcca gcccacgcgc
      181 tgccccgcgc tgcccacctg cgcgctgggg accacgccgg tgttcgacct gtgccgctgt
      241 tgccgcgtct gccccgcggc cgagcgtgaa gtctgcggcg gggcgcaggg ccaaccgtgc
      301 gccccggggc tgcagtgcct ccagccgctg cgccccgggt tccccagcac ctgcggttgc
      361 ccgacgctgg gaggggccgt gtgcggcagc gacaggcgca cctaccccag catgtgcgcg
      421 ctccgggccg aaaaccgcgc cgcgcgccgc ctgggcaagg tcccggccgt gcctgtgcag
      481 tgggggaact gcggggatac agggaccaga agcgcaggcc cgctcaggag gaattacaac
      541 ttcatcgccg cggtggtgga gaaggtggcg ccatcggtgg ttcacgtgca gctgtggggc
      601 aggttacttc acggcagcag gcttgttcct gtgtacagtg gctctgggtt catagtgtct
      661 gaggacgggc tcattattac caatgcccat gttgtcagga accagcagtg gattgaggtg
      721 gtgctccaga atggggcccg ttatgaagct gttgtcaagg atattgacct taaattggat
      781 cttgcggtga ttaagattga atcaaatgct gaacttcctg tactgatgct gggaagatca
      841 tctgaccttc gggctggaga gtttgtggtg gctttgggca gcccattttc tctgcagaac
      901 acagctactg caggaattgt cagcaccaaa cagcgagggg gcaaagaact ggggatgaag
      961 gattcagata tggactacgt ccagattgat gccacaatta attatgggaa ttctggtggt
     1021 cctctggtga acttggatgg tgatgtgatt ggcgtcaatt cattgagggt gactgatgga
     1081 atctcctttg caattccttc agatcgagtt aggcagttct tggcagaata ccatgagcac
     1141 cagatgaaag gaaaggcgtt ttcaaataag aaatatctgg gtctgcaaat gctgtccctc
     1201 actgtgcccc ttagtgaaga attgaaaatg cattatccag atttccctga tgtgagttct
     1261 ggggtttatg tatgtaaagt ggttgaagga acagctgctc aaagctctgg attgagagat
     1321 cacgatgtaa ttgtcaacat aaatgggaaa cctattacta ctacaactga tgttgttaaa
     1381 gctcttgaca gtgattccct ttccatggct gttcttcggg gaaaagataa tttgctcctg
     1441 acagtcatac ctgaaacaat caattaaata tcttgtttta aagtgggatt atctaaaaaa
     1501 aaaaaaacca gttatatcac gtggtttgta ttggagatgt gccaaacatg gcaagaagtt
     1561 tttggatctt tttcttacaa agaaaaatgg atggttatca acccaaatgc ccatcaatga
     1621 cagactggat aaagcaaatg tggtacatat acaccatgga atactatgca gccataaaaa
     1681 gcaacagtcc tctgcaggga catggatgga gctggaaacc attatcctca gcaaactaac
     1741 gcaggaacag aaaaccaaat actgcatgtt ctcacttata agtgggagct gaacaatgag
     1801 aacacatgaa catagggagg ggaacaacac acactggggc ctggcagtgg gtagggtaga
     1861 gggagggaga gcattagcaa aaatagctaa tgcatgctgg gcttaacacc caggtgatgg
     1921 gttgattgat aggtgcagca aaccatcatg gcacacattt acctatgaaa caaacctgca
     1981 catcctgcat atgtacctca gaacttaaaa ataaaaataa aaagaaaaat gg
//
