LOCUS       NM_000870               3336 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens 5-hydroxytryptamine receptor 4 (HTR4), transcript
            variant b, mRNA.
ACCESSION   NM_000870
VERSION     NM_000870.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Kohler-Forsberg,K., Ozenne,B., Larsen,S.V., Poulsen,A.S.,
            Landman,E.B., Dam,V.H., Ip,C.T., Jorgensen,A., Svarer,C.,
            Knudsen,G.M., Frokjaer,V.G. and Jorgensen,M.B.
  TITLE     Concurrent anxiety in patients with major depression and cerebral
            serotonin 4 receptor binding. A NeuroPharm-1 study
  JOURNAL   Transl Psychiatry 12 (1), 273 (2022)
   PUBMED   35821015
  REMARK    GeneRIF: Concurrent anxiety in patients with major depression and
            cerebral serotonin 4 receptor binding. A NeuroPharm-1 study.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Maillet,M., Gastineau,M., Bochet,P., Asselin-Labat,M.L., Morel,E.,
            Laverriere,J.N., Lompre,A.M., Fischmeister,R. and Lezoualc'h,F.
  TITLE     Functional studies of the 5'-untranslated region of human 5-HT4
            receptor mRNA
  JOURNAL   Biochem J 387 (Pt 2), 463-471 (2005)
   PUBMED   15575821
  REMARK    GeneRIF: Our results suggest a complex regulation of the h5-HT4
            receptor gene expression involving distinct promoters and
            non-coding exons.
REFERENCE   3  (bases 1 to 3336)
  AUTHORS   Brattelid,T., Kvingedal,A.M., Krobert,K.A., Andressen,K.W.,
            Bach,T., Hystad,M.E., Kaumann,A.J. and Levy,F.O.
  TITLE     Cloning, pharmacological characterisation and tissue distribution
            of a novel 5-HT4 receptor splice variant, 5-HT4(i)
  JOURNAL   Naunyn Schmiedebergs Arch Pharmacol 369 (6), 616-628 (2004)
   PUBMED   15118808
REFERENCE   4  (bases 1 to 3336)
  AUTHORS   Hiroi,T., Hayashi-Kobayashi,N., Nagumo,S., Ino,M., Okawa,Y.,
            Aoba,A. and Matsui,H.
  TITLE     Identification and characterization of the human serotonin-4
            receptor gene promoter
  JOURNAL   Biochem Biophys Res Commun 289 (2), 337-344 (2001)
   PUBMED   11716477
  REMARK    GeneRIF: This paper presents an analysis of the SH3TC2 promoter
            after identifying a read-through transcript of the SH3TC2 and HTR4
            loci. Available data suggests HTR4 is a separate locus with its own
            promoter, and not the product of a bi-cistronic transcript.
REFERENCE   5  (bases 1 to 3336)
  AUTHORS   Bender,E., Pindon,A., van Oers,I., Zhang,Y.B., Gommeren,W.,
            Verhasselt,P., Jurzak,M., Leysen,J. and Luyten,W.
  TITLE     Structure of the human serotonin 5-HT4 receptor gene and cloning of
            a novel 5-HT4 splice variant
  JOURNAL   J Neurochem 74 (2), 478-489 (2000)
   PUBMED   10646498
REFERENCE   6  (bases 1 to 3336)
  AUTHORS   Claeysen,S., Sebben,M., Becamel,C., Bockaert,J. and Dumuis,A.
  TITLE     Novel brain-specific 5-HT4 receptor splice variants show marked
            constitutive activity: role of the C-terminal intracellular domain
  JOURNAL   Mol Pharmacol 55 (5), 910-920 (1999)
   PUBMED   10220570
REFERENCE   7  (bases 1 to 3336)
  AUTHORS   Blondel,O., Gastineau,M., Dahmoune,Y., Langlois,M. and
            Fischmeister,R.
  TITLE     Cloning, expression, and pharmacology of four human
            5-hydroxytryptamine 4 receptor isoforms produced by alternative
            splicing in the carboxyl terminus
  JOURNAL   J Neurochem 70 (6), 2252-2261 (1998)
   PUBMED   9603189
REFERENCE   8  (bases 1 to 3336)
  AUTHORS   Cichon,S., Kesper,K., Propping,P. and Nothen,M.M.
  TITLE     Assignment of the human serotonin 4 receptor gene (HTR4) to the
            long arm of chromosome 5 (5q31-q33)
  JOURNAL   Mol Membr Biol 15 (2), 75-78 (1998)
   PUBMED   9724925
REFERENCE   9  (bases 1 to 3336)
  AUTHORS   Blondel,O., Vandecasteele,G., Gastineau,M., Leclerc,S.,
            Dahmoune,Y., Langlois,M. and Fischmeister,R.
  TITLE     Molecular and functional characterization of a 5-HT4 receptor
            cloned from human atrium
  JOURNAL   FEBS Lett 412 (3), 465-474 (1997)
   PUBMED   9276448
REFERENCE   10 (bases 1 to 3336)
  AUTHORS   Ullmer,C., Schmuck,K., Kalkman,H.O. and Lubbert,H.
  TITLE     Expression of serotonin receptor mRNAs in blood vessels
  JOURNAL   FEBS Lett 370 (3), 215-221 (1995)
   PUBMED   7656980
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008627.7, DR000612.1,
            AJ131724.1 and AC091971.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 30, 2019 this sequence version replaced NM_000870.6.
            
            Summary: This gene is a member of the family of serotonin
            receptors, which are G protein coupled receptors that stimulate
            cAMP production in response to serotonin (5-hydroxytryptamine). The
            gene product is a glycosylated transmembrane protein that functions
            in both the peripheral and central nervous system to modulate the
            release of various neurotransmitters. Multiple transcript variants
            encoding proteins with distinct C-terminal sequences have been
            described. [provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (b) encodes the longest isoform
            (b).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ131724.1, SRR1803614.139874.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2152568
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377888.8/ ENSP00000367120.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-351               AC008627.7         50243-50593
            352-383             DR000612.1         9-40
            384-2188            AJ131724.1         11-1815
            2189-2189           AC091971.4         46230-46230
            2190-3331           AJ131724.1         1816-2957
            3332-3336           AC091971.4         47373-47377
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q32"
     gene            1..3336
                     /gene="HTR4"
                     /gene_synonym="5-HT4; 5-HT4R"
                     /note="5-hydroxytryptamine receptor 4"
                     /db_xref="GeneID:3360"
                     /db_xref="HGNC:HGNC:5299"
                     /db_xref="MIM:602164"
     exon            1..466
                     /gene="HTR4"
                     /gene_synonym="5-HT4; 5-HT4R"
                     /inference="alignment:Splign:2.1.0"
     exon            467..539
                     /gene="HTR4"
                     /gene_synonym="5-HT4; 5-HT4R"
                     /inference="alignment:Splign:2.1.0"
     CDS             514..1680
                     /gene="HTR4"
                     /gene_synonym="5-HT4; 5-HT4R"
                     /note="isoform b is encoded by transcript variant b;
                     cardiac 5-HT4 receptor; 5-hydroxytryptamine (serotonin)
                     receptor 4, G protein-coupled"
                     /codon_start=1
                     /product="5-hydroxytryptamine receptor 4 isoform b"
                     /protein_id="NP_000861.1"
                     /db_xref="CCDS:CCDS4291.1"
                     /db_xref="GeneID:3360"
                     /db_xref="HGNC:HGNC:5299"
                     /db_xref="MIM:602164"
                     /translation="MDKLDANVSSEEGFGSVEKVVLLTFLSTVILMAILGNLLVMVAV
                     CWDRQLRKIKTNYFIVSLAFADLLVSVLVMPFGAIELVQDIWIYGEVFCLVRTSLDVL
                     LTTASIFHLCCISLDRYYAICCQPLVYRNKMTPLRIALMLGGCWVIPTFISFLPIMQG
                     WNNIGIIDLIEKRKFNQNSNSTYCVFMVNKPYAITCSVVAFYIPFLLMVLAYYRIYVT
                     AKEHAHQIQMLQRAGASSESRPQSADQHSTHRMRTETKAAKTLCIIMGCFCLCWAPFF
                     VTNIVDPFIDYTVPGQVWTAFLWLGYINSGLNPFLYAFLNKSFRRAFLIILCCDDERY
                     RRPSILGQTVPCSTTTINGSTHVLRDAVECGGQWESQCHPPATSPLVAAQPSDT"
     exon            540..665
                     /gene="HTR4"
                     /gene_synonym="5-HT4; 5-HT4R"
                     /inference="alignment:Splign:2.1.0"
     exon            666..866
                     /gene="HTR4"
                     /gene_synonym="5-HT4; 5-HT4R"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1020
                     /gene="HTR4"
                     /gene_synonym="5-HT4; 5-HT4R"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1589
                     /gene="HTR4"
                     /gene_synonym="5-HT4; 5-HT4R"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..3336
                     /gene="HTR4"
                     /gene_synonym="5-HT4; 5-HT4R"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agatgagcag cgctccggcc agggagggcg cgtgctggcc agcccggaac gggtgagatc
       61 gctccagccc gcctctgtgc ccactcccct gtgcgccctg ccggcagagc ggcacccccg
      121 ctgtccctgg cgcccagtgg gggcggcagc gggcagggca gcggggacca ccggggctgg
      181 gggctgttga gcccgtggag tccggctcgg ttggggagaa ggacgatgcg cggcgagccc
      241 aggtgatccg gggctggggt cctgcgcccg cagcccctgg cccgcgcctc acgctcgccg
      301 gctccccagc gcgggggctg gagcccgcac ccgagggggc ggcagggagg gcgcaggcag
      361 cggctgggag cgcagcggca gcggcggcag cagaagctcg gctcagcggc tgggggtggc
      421 cgctcgaatc tgccagggca cctcgctccc tcgcctctgg cagcgggacc ctgtgggcat
      481 tgaaatccaa ctcactcatg cttatttcct gtaatggaca aacttgatgc taatgtgagt
      541 tctgaggagg gtttcgggtc agtggagaag gtggtgctgc tcacgtttct ctcgacggtt
      601 atcctgatgg ccatcttggg gaacctgctg gtgatggtgg ctgtgtgctg ggacaggcag
      661 ctcaggaaaa taaaaacaaa ttatttcatt gtatctcttg cttttgcgga tctgctggtt
      721 tcggtgctgg tgatgccctt tggtgccatt gagctggttc aagacatctg gatttatggg
      781 gaggtgtttt gtcttgttcg gacatctctg gacgtcctgc tcacaacggc atcgattttt
      841 cacctgtgct gcatttctct ggataggtat tacgccatct gctgccagcc tttggtctat
      901 aggaacaaga tgacccctct gcgcatcgca ttaatgctgg gaggctgctg ggtcatcccc
      961 acgtttattt cttttctccc tataatgcaa ggctggaata acattggcat aattgatttg
     1021 atagaaaaga ggaagttcaa ccagaactct aactctacgt actgtgtctt catggtcaac
     1081 aagccctacg ccatcacctg ctctgtggtg gccttctaca tcccatttct cctcatggtg
     1141 ctggcctatt accgcatcta tgtcacagct aaggagcatg cccatcagat ccagatgtta
     1201 caacgggcag gagcctcctc cgagagcagg cctcagtcgg cagaccagca tagcactcat
     1261 cgcatgagga cagagaccaa agcagccaag accctgtgca tcatcatggg ttgcttctgc
     1321 ctctgctggg caccattctt tgtcaccaat attgtggatc ctttcataga ctacactgtc
     1381 cctgggcagg tgtggactgc tttcctctgg ctcggctata tcaattccgg gttgaaccct
     1441 tttctctacg ccttcttgaa taagtctttt agacgtgcct tcctcatcat cctctgctgt
     1501 gatgatgagc gctaccgaag accttccatt ctgggccaga ctgtcccttg ttcaaccaca
     1561 accattaatg gatccacaca tgtactaagg gatgcagtgg agtgtggtgg ccagtgggag
     1621 agtcagtgtc acccgccagc aacttctcct ttggtggctg ctcagcccag tgacacttag
     1681 gcccctggga caatgaccca gaagacagcc atgcctccga aagagggcca ggtcctaagc
     1741 tgctgcttgt gcgcgactgc acccggtatt ctcttcacct gaggctttcc gtccgccagt
     1801 gcaggaaccc ggtgctcgct gggcttttcc tctgagattc cagcaggtgg cgctggaggg
     1861 agtcagggga cataatggcc tccttgttca ctttttattt cccaacactc cctcttccca
     1921 gattctctgc ttttgccgcg tggtctctgg tgtctctgac atgttcccgt cactcactgt
     1981 gtctgatctg tctcactcac gttctaagca cgatagcttg ctgcgtccta tctcggtcac
     2041 tgatgtctcc aaatacgcct tctttgctgt gtgggcttcc agatatcaac aaaatactcc
     2101 ctctctgttc ctgtcttagc cagaggcgtc gctcctgtct tgcttacttg cttggtcacg
     2161 tccgtcacag ccatgttcaa gctatcccct gcctcttctt cgccctgttc tgtgccagac
     2221 gctaacacac ttttctccct gtgtctgggt ccaaacgtgc atcctcttgg ttggccccct
     2281 tgtcagtccc agacgtccac ttgctcttcc ttatgtccaa tgtctgtcct cctcttcagg
     2341 acatgctcca tttattttcc atggaagaca cagggtcaga gatctgtcag aggaaaatgg
     2401 tctccacagc aactctggag ggaagctgaa gacagatagc ttcctcctgg tgtcctggcc
     2461 gctggcgtgt ctgccctgag atgctcgctc tcctgctggg ctagaacggg acgtcttcta
     2521 aaaagccttg ggcagggcta gcaacatcgt aggtgtttat taggcttcag gatctttccc
     2581 atgaacttgt gatatcattt tgaaaccctt caatctggct ttgttgctgc caaaggagat
     2641 aactgttgga agttcacact ggggaaactc attttcccct gcagctcccc caaggtgcag
     2701 gagggcagga tccagcagca ctggggatgc cgaggcaatg ctgtacttga agccagagga
     2761 cccaaattgc cgccctggct gtgctgcaaa caagctgtga gatcttgaat agtgttcttt
     2821 catctctgga ccccactttc cacatctgtg cactcaagct tggactagag gacctttaag
     2881 ttttctttct gtttctaaga caacagtatg ctgctatagc cgtctgctac ctcgagaagc
     2941 catggcctgc ctgccttttg gctttgtttc aaaggaacga ccctcctcat ttgggccttt
     3001 tagacccctg acctttccag ctttgtcctt cagattaatc tggatgtctt ttattcaaag
     3061 ctgagtcagt ggctcttgcg aatgccccat agcagaagtc ccgggaaggt aaatgccaaa
     3121 cccagagcca tgtgagattc tgactttgat cttggctgtg gtttaatagc atctcaggat
     3181 tatttatcat attctctttt tttcttattg cttataaggt gtcagttatt gtttccattg
     3241 ttgtttactt tagttctgga actgaaaccg acaggaagaa actttggaat aaggaagaga
     3301 agattgacca aaccatccaa atgccaaaaa gaaaaa
//
