LOCUS       NM_001256613            2139 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens 5-hydroxytryptamine receptor 3E (HTR3E), transcript
            variant 4, mRNA.
ACCESSION   NM_001256613
VERSION     NM_001256613.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Lennertz,L., Wagner,M., Grabe,H.J., Franke,P.E., Guttenthaler,V.,
            Rampacher,F., Schulze-Rauschenbach,S., Vogeley,A., Benninghoff,J.,
            Ruhrmann,S., Pukrop,R., Klosterkotter,J., Falkai,P., Maier,W. and
            Mossner,R.
  TITLE     5-HT3 receptor influences the washing phenotype and visual
            organization in obsessive-compulsive disorder supporting 5-HT3
            receptor antagonists as novel treatment option
  JOURNAL   Eur Neuropsychopharmacol 24 (1), 86-94 (2014)
   PUBMED   23928294
  REMARK    GeneRIF: the HTR3E remained significant after correction for the
            number of variants investigated.
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Ripke,S., Wray,N.R., Lewis,C.M., Hamilton,S.P., Weissman,M.M.,
            Breen,G., Byrne,E.M., Blackwood,D.H., Boomsma,D.I., Cichon,S.,
            Heath,A.C., Holsboer,F., Lucae,S., Madden,P.A., Martin,N.G.,
            McGuffin,P., Muglia,P., Noethen,M.M., Penninx,B.P., Pergadia,M.L.,
            Potash,J.B., Rietschel,M., Lin,D., Muller-Myhsok,B., Shi,J.,
            Steinberg,S., Grabe,H.J., Lichtenstein,P., Magnusson,P.,
            Perlis,R.H., Preisig,M., Smoller,J.W., Stefansson,K., Uher,R.,
            Kutalik,Z., Tansey,K.E., Teumer,A., Viktorin,A., Barnes,M.R.,
            Bettecken,T., Binder,E.B., Breuer,R., Castro,V.M., Churchill,S.E.,
            Coryell,W.H., Craddock,N., Craig,I.W., Czamara,D., De Geus,E.J.,
            Degenhardt,F., Farmer,A.E., Fava,M., Frank,J., Gainer,V.S.,
            Gallagher,P.J., Gordon,S.D., Goryachev,S., Gross,M., Guipponi,M.,
            Henders,A.K., Herms,S., Hickie,I.B., Hoefels,S., Hoogendijk,W.,
            Hottenga,J.J., Iosifescu,D.V., Ising,M., Jones,I., Jones,L.,
            Jung-Ying,T., Knowles,J.A., Kohane,I.S., Kohli,M.A., Korszun,A.,
            Landen,M., Lawson,W.B., Lewis,G., Macintyre,D., Maier,W.,
            Mattheisen,M., McGrath,P.J., McIntosh,A., McLean,A.,
            Middeldorp,C.M., Middleton,L., Montgomery,G.M., Murphy,S.N.,
            Nauck,M., Nolen,W.A., Nyholt,D.R., O'Donovan,M., Oskarsson,H.,
            Pedersen,N., Scheftner,W.A., Schulz,A., Schulze,T.G., Shyn,S.I.,
            Sigurdsson,E., Slager,S.L., Smit,J.H., Stefansson,H., Steffens,M.,
            Thorgeirsson,T., Tozzi,F., Treutlein,J., Uhr,M., van den Oord,E.J.,
            Van Grootheest,G., Volzke,H., Weilburg,J.B., Willemsen,G.,
            Zitman,F.G., Neale,B., Daly,M., Levinson,D.F. and Sullivan,P.F.
  CONSRTM   Major Depressive Disorder Working Group of the Psychiatric GWAS
            Consortium
  TITLE     A mega-analysis of genome-wide association studies for major
            depressive disorder
  JOURNAL   Mol Psychiatry 18 (4), 497-511 (2013)
   PUBMED   22472876
REFERENCE   3  (bases 1 to 2139)
  AUTHORS   Kapeller,J., Moller,D., Lasitschka,F., Autschbach,F., Hovius,R.,
            Rappold,G., Bruss,M., Gershon,M.D. and Niesler,B.
  TITLE     Serotonin receptor diversity in the human colon: Expression of
            serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E
  JOURNAL   J Comp Neurol 519 (3), 420-432 (2011)
   PUBMED   21192076
  REMARK    GeneRIF: Data show that 5-HT3C, 5-HT3D, and 5-HT3E subunits are
            coexpressed with 5-HT3A in cell bodies of myenteric neurons, and
            that 5-HT3A and 5-HT3D were expressed in submucosal plexus of the
            human large intestine.
REFERENCE   4  (bases 1 to 2139)
  AUTHORS   Hammer,C., Fasching,P.A., Loehberg,C.R., Rauh,C., Ekici,A.B.,
            Jud,S.M., Bani,M.R., Beckmann,M.W., Strick,R. and Niesler,B.
  TITLE     Polymorphism in HTR3D shows different risks for acute
            chemotherapy-induced vomiting after anthracycline chemotherapy
  JOURNAL   Pharmacogenomics 11 (7), 943-950 (2010)
   PUBMED   20602613
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   5  (bases 1 to 2139)
  AUTHORS   Nalivaiko,E. and Sgoifo,A.
  TITLE     Central 5-HT receptors in cardiovascular control during stress
  JOURNAL   Neurosci Biobehav Rev 33 (2), 95-106 (2009)
   PUBMED   18573276
  REMARK    GeneRIF: Data show that 5-HT3 receptors locat in the nucleus
            tractus silitarius contribute to stress-induced suppression of the
            baroreflex.
            Review article
REFERENCE   6  (bases 1 to 2139)
  AUTHORS   Kapeller,J., Houghton,L.A., Monnikes,H., Walstab,J., Moller,D.,
            Bonisch,H., Burwinkel,B., Autschbach,F., Funke,B., Lasitschka,F.,
            Gassler,N., Fischer,C., Whorwell,P.J., Atkinson,W., Fell,C.,
            Buchner,K.J., Schmidtmann,M., van der Voort,I., Wisser,A.S.,
            Berg,T., Rappold,G. and Niesler,B.
  TITLE     First evidence for an association of a functional variant in the
            microRNA-510 target site of the serotonin receptor-type 3E gene
            with diarrhea predominant irritable bowel syndrome
  JOURNAL   Hum Mol Genet 17 (19), 2967-2977 (2008)
   PUBMED   18614545
  REMARK    GeneRIF: MicroRNA-510 target site of the 3'UTR of HTR3A and HTR3E
            are associated with the irritable bowel syndrome with diarrhea.
            GeneRIF: The binding site for miR-510 is solely located in the 3'
            UTR of the HTR3E gene. No predicted binding site for miR-510 exists
            in the 3' UTR of HTR3A.
REFERENCE   7  (bases 1 to 2139)
  AUTHORS   Niesler,B., Walstab,J., Combrink,S., Moller,D., Kapeller,J.,
            Rietdorf,J., Bonisch,H., Gothert,M., Rappold,G. and Bruss,M.
  TITLE     Characterization of the novel human serotonin receptor subunits
            5-HT3C,5-HT3D, and 5-HT3E
  JOURNAL   Mol Pharmacol 72 (1), 8-17 (2007)
   PUBMED   17392525
REFERENCE   8  (bases 1 to 2139)
  AUTHORS   Peters,J.A., Kelley,S.P., Dunlop,J.I., Kirkness,E.F., Hales,T.G.
            and Lambert,J.J.
  TITLE     The 5-hydroxytryptamine type 3 (5-HT3) receptor reveals a novel
            determinant of single-channel conductance
  JOURNAL   Biochem Soc Trans 32 (Pt3), 547-552 (2004)
   PUBMED   15157181
  REMARK    Review article
REFERENCE   9  (bases 1 to 2139)
  AUTHORS   Karnovsky,A.M., Gotow,L.F., McKinley,D.D., Piechan,J.L.,
            Ruble,C.L., Mills,C.J., Schellin,K.A., Slightom,J.L.,
            Fitzgerald,L.R., Benjamin,C.W. and Roberds,S.L.
  TITLE     A cluster of novel serotonin receptor 3-like genes on human
            chromosome 3
  JOURNAL   Gene 319, 137-148 (2003)
   PUBMED   14597179
REFERENCE   10 (bases 1 to 2139)
  AUTHORS   Niesler,B., Frank,B., Kapeller,J. and Rappold,G.A.
  TITLE     Cloning, physical mapping and expression analysis of the human
            5-HT3 serotonin receptor-like genes HTR3C, HTR3D and HTR3E
  JOURNAL   Gene 310, 101-111 (2003)
   PUBMED   12801637
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DQ644022.1 and AC131235.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 13, 2020 this sequence version replaced NM_001256613.1.
            
            Summary: This locus encodes a 5-hydroxytryptamine (serotonin)
            receptor subunit. The encoded protein, subunit E, may play a role
            in neurotransmission in myenteric neurons. Genes encoding subunits
            C, D and E form a cluster on chromosome 3. Alternatively spliced
            transcript variants encoding distinct isoforms have been described.
            [provided by RefSeq, Feb 2012].
            
            Transcript Variant: This variant (4) has multiple differences in
            the 5' UTR and coding region, compared to variant 1. These
            differences result in translation initiation at an alternate start
            codon, compared to variant 1. The encoded isoform (d) has a
            distinct N-terminus and is shorter than isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ644022.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA2142348 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000415389.6/ ENSP00000401444.2
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-334               DQ644022.1         1-334
            335-335             AC131235.4         114409-114409       c
            336-394             DQ644022.1         336-394
            395-395             AC131235.4         114349-114349       c
            396-1209            DQ644022.1         396-1209
            1210-1210           AC131235.4         106019-106019       c
            1211-2139           DQ644022.1         1211-2139
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q27.1"
     gene            1..2139
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /note="5-hydroxytryptamine receptor 3E"
                     /db_xref="GeneID:285242"
                     /db_xref="HGNC:HGNC:24005"
                     /db_xref="MIM:610123"
     exon            1..533
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /inference="alignment:Splign:2.1.0"
     CDS             467..1837
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /note="isoform d precursor is encoded by transcript
                     variant 4; 5-hydroxytryptamine receptor 3 subunit E;
                     5-hydroxytryptamine (serotonin) receptor 3, family member
                     E; 5-hydroxytryptamine (serotonin) receptor 3E,
                     ionotropic"
                     /codon_start=1
                     /product="5-hydroxytryptamine receptor 3E isoform d
                     precursor"
                     /protein_id="NP_001243542.1"
                     /db_xref="CCDS:CCDS58868.1"
                     /db_xref="GeneID:285242"
                     /db_xref="HGNC:HGNC:24005"
                     /db_xref="MIM:610123"
                     /translation="MEGSWFHRKRFSFYLLLGFLLQGRGVTFTINCSGFGQHGADPTA
                     LNSVFNRKPFRPVTNISVPTQVNISFAMSAILDVNEQLHLLSSFLWLEMVWDNPFISW
                     NPEECEGITKMSMAAKNLWLPDIFIIELMDVDKTPKGLTAYVSNEGRIRYKKPMKVDS
                     ICNLDIFYFPFDQQNCTLTFSSFLYTVDSMLLDMEKEVWEITDASRNILQTHGEWELL
                     GLSKATAKLSRGGNLYDQIVFYVAIRRRPSLYVINLLVPSGFLVAIDALSFYLPVKSG
                     NRVPFKITLLLGYNVFLLMMSDLLPTSGTPLIGVYFALCLSLMVGSLLETIFITHLLH
                     VATTQPPPLPRWLHSLLLHCNSPGRCCPTAPQKENKGPGLTPTHLPGVKEPEVSAGQM
                     PGPAEAELTGGSEWTRAQREHEAQKQHSVELWLQFSHAMDAMLFRLYLLFMASSIITV
                     ICLWNT"
     exon            534..700
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /inference="alignment:Splign:2.1.0"
     exon            701..745
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /inference="alignment:Splign:2.1.0"
     exon            746..855
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /inference="alignment:Splign:2.1.0"
     exon            856..1025
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1186
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1391
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1607
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..2139
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2120..2125
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /note="hexamer: AATAAA"
     polyA_site      2139
                     /gene="HTR3E"
                     /gene_synonym="5-HT3-E; 5-HT3c1; 5-HT3E"
                     /note="major polyA site"
ORIGIN      
        1 gtaaaataac aaattgtgtt tatttaagcc actgggtttg tgatttgtta caacagcaat
       61 aaaaggcatg taagatagaa ggaaaccatt caacacatgt ctaataagaa aaatagagga
      121 agtgaaacat ttataaatgt ctgagaattt tccaaaattg aagagatgag tcttcagacc
      181 aagggatctc accaagatct gcaagttgat cacgtatgaa ctatctataa ccatttacgt
      241 tgcagtgaaa tacctaagac taagataaag tcttaaaagc agactgaacc ttaagcatag
      301 gcaagaacaa gacaaccaga acactctttg aaggaaggtt acaaatgtca gtggtcaacc
      361 aatgctatta gtattcaaag tcagactcta ggtgtggcct gtgctgcttt aatctgggca
      421 ttcctgggtc ccagtacatg ttcagagaag aacttagaca aagaagatgg aaggaagctg
      481 gttccacagg aaaagatttt ccttctacct ccttctcggt tttctgcttc aaggaagggg
      541 cgttactttc accatcaatt gctcagggtt tggccagcac ggggcggatc ccactgctct
      601 gaattcagtg tttaatagaa agcccttccg tccggtcacc aacatcagcg tccccaccca
      661 agtcaacatc tccttcgcga tgtctgccat cctagatgtg aatgaacagc tgcacctctt
      721 gtcatcattc ctgtggctgg aaatggtttg ggataaccca tttatcagct ggaacccaga
      781 ggaatgtgag ggcatcacga agatgagtat ggcagccaag aacctgtggc tcccagacat
      841 tttcatcatt gaactcatgg atgtggataa gaccccaaaa ggcctcacag catatgtaag
      901 taatgaaggt cgcatcaggt ataagaaacc catgaaggtg gacagtatct gtaacctgga
      961 catcttctac ttccccttcg accagcagaa ctgcacactc accttcagct cattcctcta
     1021 cacagtggac agcatgttgc tggacatgga gaaagaagtg tgggaaataa cagacgcatc
     1081 ccggaacatc cttcagaccc atggagaatg ggagctcctg ggcctcagca aggccaccgc
     1141 aaagttgtcc aggggaggca acctgtatga tcagatcgtg ttctatgtgg ccatcaggcg
     1201 caggcccagt ctctatgtca taaaccttct cgtgcccagt ggctttctgg ttgccatcga
     1261 tgccctcagc ttctacctgc cagtgaaaag tgggaatcgt gtcccattca agataacgct
     1321 cctgctgggc tacaacgtct tcctgctcat gatgagtgac ttgctcccca ccagtggcac
     1381 ccccctcatc ggtgtctact tcgccctgtg cctgtccctg atggtgggca gcctgctgga
     1441 gaccatcttc atcacccacc tgctgcacgt ggccaccacc cagcccccac ccctgcctcg
     1501 gtggctccac tccctgctgc tccactgcaa cagcccgggg agatgctgtc ccactgcgcc
     1561 ccagaaggaa aataagggcc cgggtctcac ccccacccac ctgcccggtg tgaaggagcc
     1621 agaggtatca gcagggcaga tgccgggccc tgcggaggca gagctgacag ggggctcaga
     1681 atggacaagg gcccagcggg aacacgaggc ccagaagcag cactcagtgg agctgtggtt
     1741 gcagttcagc cacgcgatgg acgccatgct cttccgcctc tacctgctct tcatggcctc
     1801 ctctatcatc accgtcatat gcctctggaa cacctaggca ggtgctcacc tgccaacttc
     1861 agtctggagc ttctcttgcc tccagggact ggccaggtct cccccctttc ctgagtacca
     1921 actatcatat ccccaaagat gactgagtct ctgctgtatt ccatgtatcc caatccggtc
     1981 ctgctgatca attccaatcc cagacatttc tccctgttcc tgcattttgt tggcttcctt
     2041 cagtcctacc atatggttct aggtccctct tacgtcatct gcatagcaga ctatacctct
     2101 tctgtccgct gacttgccca ataaataatt ctgcagaga
//
