LOCUS       NM_000869               2205 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens 5-hydroxytryptamine receptor 3A (HTR3A), transcript
            variant 2, mRNA.
ACCESSION   NM_000869
VERSION     NM_000869.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Nikolic,J., Vukojevic,K., Soljic,V., Miskovic,J., Orlovic Vlaho,M.,
            Saraga-Babic,M. and Filipovic,N.
  TITLE     Expression Patterns of Serotonin Receptors 5-HT1A, 5-HT2A, and
            5-HT3A during Human Fetal Lung Development
  JOURNAL   Int J Mol Sci 24 (3), 2965 (2023)
   PUBMED   36769290
  REMARK    GeneRIF: Expression Patterns of Serotonin Receptors 5-HT1A, 5-HT2A,
            and 5-HT3A during Human Fetal Lung Development.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Berens,S., Dong,Y., Fritz,N., Walstab,J., D'Amato,M., Zheng,T.,
            Wahl,V., Boekstegers,F., Bermejo,J.L., Martinez,C.,
            Schmitteckert,S., Clevers,E., Engel,F., Gauss,A., Herzog,W.,
            Spiller,R., Goebel-Stengel,M., Monnikes,H., Andresen,V., Thomas,F.,
            Keller,J., Pehl,C., Stein-Thoringer,C., Clarke,G., Dinan,T.G.,
            Quigley,E.M., Sayuk,G., Simren,M., Tesarz,J., Rappold,G., van
            Oudenhove,L., Schaefert,R. and Niesler,B.
  TITLE     Serotonin type 3 receptor subunit gene polymorphisms associated
            with psychosomatic symptoms in irritable bowel syndrome: A
            multicenter retrospective study
  JOURNAL   World J Gastroenterol 28 (21), 2334-2349 (2022)
   PUBMED   35800179
  REMARK    GeneRIF: Serotonin type 3 receptor subunit gene polymorphisms
            associated with psychosomatic symptoms in irritable bowel syndrome:
            A multicenter retrospective study.
REFERENCE   3  (bases 1 to 2205)
  AUTHORS   Olatunji,B.O., Cox,R.C. and Blackford,J.U.
  TITLE     Fear reacquisition and symptoms of combat-related PTSD: Specificity
            and preliminary examination of the influence of the 5-HT3A receptor
            gene
  JOURNAL   Behav Res Ther 153, 104085 (2022)
   PUBMED   35413654
  REMARK    GeneRIF: Fear reacquisition and symptoms of combat-related PTSD:
            Specificity and preliminary examination of the influence of the
            5-HT3A receptor gene.
REFERENCE   4  (bases 1 to 2205)
  AUTHORS   Ritter,K.E., Buehler,D.P., Asher,S.B., Deal,K.K., Zhao,S., Guo,Y.
            and Southard-Smith,E.M.
  TITLE     5-HT3 Signaling Alters Development of Sacral Neural Crest
            Derivatives That Innervate the Lower Urinary Tract
  JOURNAL   Int J Mol Sci 22 (13), 6838 (2021)
   PUBMED   34202161
  REMARK    GeneRIF: 5-HT3 Signaling Alters Development of Sacral Neural Crest
            Derivatives That Innervate the Lower Urinary Tract.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2205)
  AUTHORS   Basak,S., Kumar,A., Ramsey,S., Gibbs,E., Kapoor,A., Filizola,M. and
            Chakrapani,S.
  TITLE     High-resolution structures of multiple 5-HT3AR-setron complexes
            reveal a novel mechanism of competitive inhibition
  JOURNAL   Elife 9, e57870 (2020)
   PUBMED   33063666
  REMARK    GeneRIF: High-resolution structures of multiple 5-HT3AR-setron
            complexes reveal a novel mechanism of competitive inhibition.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2205)
  AUTHORS   Niesler,B., Flohr,T., Nothen,M.M., Fischer,C., Rietschel,M.,
            Franzek,E., Albus,M., Propping,P. and Rappold,G.A.
  TITLE     Association between the 5' UTR variant C178T of the serotonin
            receptor gene HTR3A and bipolar affective disorder
  JOURNAL   Pharmacogenetics 11 (6), 471-475 (2001)
   PUBMED   11505217
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 2205)
  AUTHORS   Belelli,D., Balcarek,J.M., Hope,A.G., Peters,J.A., Lambert,J.J. and
            Blackburn,T.P.
  TITLE     Cloning and functional expression of a human 5-hydroxytryptamine
            type 3AS receptor subunit
  JOURNAL   Mol Pharmacol 48 (6), 1054-1062 (1995)
   PUBMED   8848005
REFERENCE   8  (bases 1 to 2205)
  AUTHORS   Weiss,B., Mertz,A., Schrock,E., Koenen,M. and Rappold,G.
  TITLE     Assignment of a human homolog of the mouse Htr3 receptor gene to
            chromosome 11q23.1-q23.2
  JOURNAL   Genomics 29 (1), 304-305 (1995)
   PUBMED   8530095
REFERENCE   9  (bases 1 to 2205)
  AUTHORS   Miyake,A., Mochizuki,S., Takemoto,Y. and Akuzawa,S.
  TITLE     Molecular cloning of human 5-hydroxytryptamine3 receptor:
            heterogeneity in distribution and function among species
  JOURNAL   Mol Pharmacol 48 (3), 407-416 (1995)
   PUBMED   7565620
REFERENCE   10 (bases 1 to 2205)
  AUTHORS   Uetz,P., Abdelatty,F., Villarroel,A., Rappold,G., Weiss,B. and
            Koenen,M.
  TITLE     Organisation of the murine 5-HT3 receptor gene and assignment to
            human chromosome 11
  JOURNAL   FEBS Lett 339 (3), 302-306 (1994)
   PUBMED   8112471
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK304630.1, AP000908.4 and
            BC002354.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 27, 2018 this sequence version replaced NM_000869.5.
            
            Summary: The product of this gene belongs to the ligand-gated ion
            channel receptor superfamily. This gene encodes subunit A of the
            type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic
            hormone that functions as a neurotransmitter, a hormone, and a
            mitogen. This receptor causes fast, depolarizing responses in
            neurons after activation. It appears that the heteromeric
            combination of A and B subunits is necessary to provide the full
            functional features of this receptor, since either subunit alone
            results in receptors with very low conductance and response
            amplitude. Alternatively spliced transcript variants encoding
            different isoforms have been identified. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (2) lacks an in-frame segment of
            the coding region, compared to variant 1. It encodes a shorter
            isoform (b), that is missing an internal segment compared to
            isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D49394.1, BC004453.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA2150385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000504030.7/
                                                ENSP00000424189.2
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation, expression
            regulatory uORF                  :: PMID: 11505217
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1509              AK304630.1         36-1544
            1510-2038           AP000908.4         39832-40360
            2039-2205           BC002354.2         1888-2054
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.2"
     gene            1..2205
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /note="5-hydroxytryptamine receptor 3A"
                     /db_xref="GeneID:3359"
                     /db_xref="HGNC:HGNC:5297"
                     /db_xref="MIM:182139"
     exon            1..285
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      132..182
                     /regulatory_class="uORF"
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:11505217]"
                     /note="this uORF is predicted to encode a 16 aa peptide
                     (PMID:11505217)"
     CDS             219..1655
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /note="isoform b precursor is encoded by transcript
                     variant 2; serotonin-gated ion channel receptor; 5HT3
                     serotonin receptor; serotonin receptor 3A;
                     5-hydroxytryptamine receptor 3; 5-hydroxytryptamine
                     (serotonin) receptor 3A, ionotropic"
                     /codon_start=1
                     /product="5-hydroxytryptamine receptor 3A isoform b
                     precursor"
                     /protein_id="NP_000860.3"
                     /db_xref="CCDS:CCDS8365.3"
                     /db_xref="GeneID:3359"
                     /db_xref="HGNC:HGNC:5297"
                     /db_xref="MIM:182139"
                     /translation="MLLWVQQALLALLLPTLLAQGEARRSRNTTRPALLRLSDYLLTN
                     YRKGVRPVRDWRKPTTVSIDVIVYAILNVDEKNQVLTTYIWYRQYWTDEFLQWNPEDF
                     DNITKLSIPTDSIWVPDILINEFVDVGKSPNIPYVYIRHQGEVQNYKPLQVVTACSLD
                     IYNFPFDVQNCSLTFTSWLHTIQDINISLWRLPEKVKSDRSVFMNQGEWELLGVLPYF
                     REFSMESSNYYAEMKFYVVIRRRPLFYVVSLLLPSIFLMVMDIVGFYLPPNSGERVSF
                     KITLLLGYSVFLIIVSDTLPATAIGTPLIGVYFVVCMALLVISLAETIFIVRLVHKQD
                     LQQPVPAWLRHLVLERIAWLLCLREQSTSQRPPATSQATKTDDCSAMGNHCSHMGGPQ
                     DFEKSPRDRCSPPPPPREASLAVCGLLQELSSIRQFLEKRDEIREVARDWLRVGSVLD
                     KLLFHIYLLAVLAYSITLVMLWSIWQYA"
     exon            286..437
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /inference="alignment:Splign:2.1.0"
     exon            438..482
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /inference="alignment:Splign:2.1.0"
     exon            483..592
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /inference="alignment:Splign:2.1.0"
     exon            593..762
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /inference="alignment:Splign:2.1.0"
     exon            763..923
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1134
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1356
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..2205
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2182..2187
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /note="hexamer: AATAAA"
     polyA_site      2205
                     /gene="HTR3A"
                     /gene_synonym="5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3"
                     /note="major polyA site"
ORIGIN      
        1 gaaacatgat ccagctgaag gactgattgc aggaaaactt ggcagctccc caaccttggt
       61 ggcccaggga gtgtgaggct gcagcctcag aaggtgtgag cagtggccac gagaggcagg
      121 ctggctggga catgaggttg gcagagggca ggcaagctgg cccttggtgg gcctcgtcct
      181 gagcactcgg aggcactcct atgcttggaa agctcgctat gctgctgtgg gtccagcagg
      241 cgctgctcgc cttgctcctc cccacactcc tggcacaggg agaagccagg aggagccgaa
      301 acaccaccag gcccgctctg ctgaggctgt cggattacct tttgaccaac tacaggaagg
      361 gtgtgcgccc cgtgagggac tggaggaagc caaccaccgt atccattgac gtcattgtct
      421 atgccatcct caacgtggat gagaagaatc aggtgctgac cacctacatc tggtaccggc
      481 agtactggac tgatgagttt ctccagtgga accctgagga ctttgacaac atcaccaagt
      541 tgtccatccc cacggacagc atctgggtcc cggacattct catcaatgag ttcgtggatg
      601 tggggaagtc tccaaatatc ccgtacgtgt atattcggca tcaaggcgaa gttcagaact
      661 acaagcccct tcaggtggtg actgcctgta gcctcgacat ctacaacttc cccttcgatg
      721 tccagaactg ctcgctgacc ttcaccagtt ggctgcacac catccaggac atcaacatct
      781 ctttgtggcg cttgccagaa aaggtgaaat ccgacaggag tgtcttcatg aaccagggag
      841 agtgggagtt gctgggggtg ctgccctact ttcgggagtt cagcatggaa agcagtaact
      901 actatgcaga aatgaagttc tatgtggtca tccgccggcg gcccctcttc tatgtggtca
      961 gcctgctact gcccagcatc ttcctcatgg tcatggacat cgtgggcttc tacctgcccc
     1021 ccaacagtgg cgagagggtc tctttcaaga ttacactcct cctgggctac tcggtcttcc
     1081 tgatcatcgt ttctgacacg ctgccggcca ctgccatcgg cactcctctc attggtgtct
     1141 actttgtggt gtgcatggct ctgctggtga taagtttggc cgagaccatc ttcattgtgc
     1201 ggctggtgca caagcaagac ctgcagcagc ccgtgcctgc ttggctgcgt cacctggttc
     1261 tggagagaat cgcctggcta ctttgcctga gggagcagtc aacttcccag aggcccccag
     1321 ccacctccca agccaccaag actgatgact gctcagccat gggaaaccac tgcagccaca
     1381 tgggaggacc ccaggacttc gagaagagcc cgagggacag atgtagccct cccccaccac
     1441 ctcgggaggc ctcgctggcg gtgtgtgggc tgctgcagga gctgtcctcc atccggcaat
     1501 tcctggaaaa gcgggatgag atccgagagg tggcccgaga ctggctgcgc gtgggctccg
     1561 tgctggacaa gctgctattc cacatttacc tgctagcggt gctggcctac agcatcaccc
     1621 tggttatgct ctggtccatc tggcagtacg cttgagtggg tacagcccag tggaggaggg
     1681 ggtacagtcc tggttaggtg gggacagagg atttctgctt aggcccctca ggacccaggg
     1741 aatgccaggg acattttcaa gacacagaca aagtcccgtg ccctgtttcc aatgccaatt
     1801 catctcagca atcacaagcc aaggtctgaa cccttccacc aaaaactggg tgttcaaggc
     1861 ccttacaccc ttgtcccacc cccagcagct caccatggct ttaaaacatg ctgtcttaga
     1921 tcaggagaaa ctcgggcact ccctaagtcc actctagttg tggacttttc cccattgacc
     1981 ctcacctgaa taagggactt tggaattctg cttctctttc acaactttgc ttttaggttg
     2041 aaggcaaaac caactctcta ctacacaggc ctgataactc tgtacgaggc ttctctaacc
     2101 cctagtgtct tttttttctt cacctcactt gtggcagctt ccctgaacac tcatccccca
     2161 tcagatgatg ggagtgggaa gaataaaatg cagtgaaacc ctaaa
//
